The G Protein-Coupled Receptor RAI3 Is an Independent Prognostic Factor for Pancreatic Cancer Survival and Regulates Proliferation via STAT3 Phosphorylation

Jahny E, Yang H, Liu B, Jahnke B, Lademann F, Knoesel T, Ruemmele P, Grützmann R, Aust DE, Pilarsky C, Denz A (2017)

Publication Type: Journal article

Publication year: 2017

Journal

PLoS ONE Public Library of Science

Book Volume: 12

Pages Range: e0170390

Journal Issue: 1

DOI: 10.1371/journal.pone.0170390

Abstract

Pancreatic Ductal Adenocarcinoma (PDAC) is one of the deadliest tumors worldwide. Understanding the function of gene expression alterations is a prerequisite for developing new strategies in diagnostic and therapy. GPRC5A (RAI3), coding for a seven transmembrane G protein-coupled receptor is known to be overexpressed in pancreatic cancer and might be an interesting candidate for therapeutic intervention. Expression levels of RAI3 were compared using a tissue microarray of 435 resected patients with pancreatic cancer as well as 209 samples from chronic pancreatitis (CP), intra-ductal papillary mucinous neoplasm (IPMN) and normal pancreatic tissue. To elucidate the function of RAI3 overexpression, siRNA based knock-down was used and transfected cells were analyzed using proliferation and migration assays. Pancreatic cancer patients showed a statistically significant overexpression of RAI3 in comparison to normal and chronic pancreatitis tissue. Especially the loss of apical RAI3 expression represents an independent prognostic parameter for overall survival of patients with pancreatic cancer. Suppression of GPRC5a results in decreased cell growth, proliferation and migration in pancreatic cancer cell lines via a STAT3 modulated pathway, independent from ERK activation.

Authors with CRIS profile

Hai Yang Professur für Chirurgische Forschung Bin Liu Professur für Chirurgische Forschung Robert Grützmann Department of Surgery Christian Pilarsky Professur für Chirurgische Forschung

Involved external institutions

Technische Universität Dresden DE Germany (DE) Ludwig-Maximilians-Universität (LMU) DE Germany (DE)

How to cite

APA:

Jahny, E., Yang, H., Liu, B., Jahnke, B., Lademann, F., Knoesel, T.,... Denz, A. (2017). The G Protein-Coupled Receptor RAI3 Is an Independent Prognostic Factor for Pancreatic Cancer Survival and Regulates Proliferation via STAT3 Phosphorylation. PLoS ONE, 12(1), e0170390. https://dx.doi.org/10.1371/journal.pone.0170390

MLA:

Jahny, Elisabeth, et al. "The G Protein-Coupled Receptor RAI3 Is an Independent Prognostic Factor for Pancreatic Cancer Survival and Regulates Proliferation via STAT3 Phosphorylation." PLoS ONE 12.1 (2017): e0170390.

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