Generation and characterization of mice for conditional inactivation of Zeb1

Brabletz S, Lasierra Losada M, Schmalhofer O, Mitschke J, Krebs A, Brabletz T, Stemmler M (2017)

Publication Type: Journal article

Publication year: 2017

Journal

Genesis Wiley-Blackwell

DOI: 10.1002/dvg.23024

Abstract

The multizinc finger containing transcription factor ZEB1 plays crucial roles during various aspects of mammalian development and tumorigenesis. Best studied in human tumors, ZEB1 is activating the embryo-derived program of epithelial-mesenchymal transition (EMT). The aberrant activation of EMT confers an invasive metastasizing phenotype with acquisition of stem cell properties and resistance to radio- and chemotherapy. Although ZEB1 has very important functions in tumor progression, not much is known about its role in physiological contexts and during development and homeostasis. We describe the generation of Zeb1(flox/flox) mice carrying a targeted mutation for conditional Zeb1 gene inactivation and show that homozygous Zeb1-depletion in the germline results in a phenotype similar to the conventional Zeb1 knockout.

Authors with CRIS profile

Simone Brabletz Lehrstuhl für Experimentelle Medizin II (Molekulare Tumorforschung) Maria Lasierra Losada Lehrstuhl für Experimentelle Medizin mit dem Schwerpunkt Molekulare Pathogeneseforschung Angela Krebs Lehrstuhl für Experimentelle Medizin mit dem Schwerpunkt Molekulare Pathogeneseforschung Thomas Brabletz Lehrstuhl für Experimentelle Medizin mit dem Schwerpunkt Molekulare Pathogeneseforschung Marc Stemmler Lehrstuhl für Experimentelle Medizin mit dem Schwerpunkt Molekulare Pathogeneseforschung

Involved external institutions

Albert-Ludwigs-Universität Freiburg DE Germany (DE)

How to cite

APA:

Brabletz, S., Lasierra Losada, M., Schmalhofer, O., Mitschke, J., Krebs, A., Brabletz, T., & Stemmler, M. (2017). Generation and characterization of mice for conditional inactivation of Zeb1. Genesis. https://doi.org/10.1002/dvg.23024

MLA:

Brabletz, Simone, et al. "Generation and characterization of mice for conditional inactivation of Zeb1." Genesis (2017).

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