Elevated vitreous body glial fibrillary acidic protein in retinal diseases

Jünemann A, Rejdak R, Huchzermeyer C, Maciejewski R, Grieb P, Kruse F, Zrenner E, Rejdak K, Petzold A (2015)

Publication Type: Journal article

Publication year: 2015

Journal

Graefes Archive For Clinical and Experimental Ophthalmology Springer Verlag (Germany)

Book Volume: 253

Pages Range: 2181-6

Journal Issue: 12

DOI: 10.1007/s00417-015-3127-7

Abstract

Increased expression of glial fibrillary acidic protein (GFAP) is a characteristic of gliotic activation (Müller cells and astrocytes) in the retina. This study assessed vitreous body GFAP levels in various forms of retinal pathology.This prospective study included 82 patients who underwent vitrectomy (46 retinal detachments (RDs), 13 macular hole (MHs), 15 epiretinal glioses (EGs), 8 organ donors). An established enzyme-linked immunosorbent assay (ELISA, SMI26) was used for quantification of GFAP.The highest concentration of vitreous body GFAP in organ donors was 20 pg/mL and it was used as the cutoff. A significant proportion of patients suffering from RD (65 %) to EG (53 %) had vitreous body GFAP levels above this cutoff when compared to organ donors (0 %, p < 0.0001, p = 0.0194, respectively, Fisher's exact test) and MH (8 %, p < 0.0001, p = 0.0157, respectively). In RD and EG, vitreous body GFAP levels were correlated with axial length (R = 0.69, R = 0.52, p < 0.05 for both).The data suggest that human vitreous body GFAP is a protein biomarker for glial activation in response to retinal pathologies. Vitreous body GFAP levels may be of interest as a surrogate outcome for experimental treatment strategies in translational studies.

Authors with CRIS profile

Anselm Jünemann Medizinische Fakultät Cord Huchzermeyer Department of Ophthalmology Friedrich Kruse Lehrstuhl für Augenheilkunde

Involved external institutions

Vrije Universiteit Amsterdam (VU) / University Amsterdam NL Netherlands (NL) Polska Akademia Nauk (PAN) / Polish Academy of Sciences PL Poland (PL) Medical University of Lublin / Uniwersytet Medyczny w Lublinie PL Poland (PL)

How to cite

APA:

Jünemann, A., Rejdak, R., Huchzermeyer, C., Maciejewski, R., Grieb, P., Kruse, F.,... Petzold, A. (2015). Elevated vitreous body glial fibrillary acidic protein in retinal diseases. Graefes Archive For Clinical and Experimental Ophthalmology, 253(12), 2181-6. https://doi.org/10.1007/s00417-015-3127-7

MLA:

Jünemann, Anselm, et al. "Elevated vitreous body glial fibrillary acidic protein in retinal diseases." Graefes Archive For Clinical and Experimental Ophthalmology 253.12 (2015): 2181-6.

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