Genotoxicity of Superparamagnetic Iron Oxide Nanoparticles in Granulosa Cells

Journal article


Publication Details

Author(s): Poettler M, Staicu A, Zaloga J, Unterweger H, Weigel B, Schreiber E, Hofmann S, Wiest I, Jeschke U, Alexiou C, Janko C
Journal: International Journal of Molecular Sciences
Publication year: 2015
Volume: 16
Journal issue: 11
Pages range: 26280-90
ISSN: 1422-0067


Abstract


Nanoparticles that are aimed at targeting cancer cells, but sparing healthy tissue provide an attractive platform of implementation for hyperthermia or as carriers of chemotherapeutics. According to the literature, diverse effects of nanoparticles relating to mammalian reproductive tissue are described. To address the impact of nanoparticles on cyto- and genotoxicity concerning the reproductive system, we examined the effect of superparamagnetic iron oxide nanoparticles (SPIONs) on granulosa cells, which are very important for ovarian function and female fertility. Human granulosa cells (HLG-5) were treated with SPIONs, either coated with lauric acid (SEONLA) only, or additionally with a protein corona of bovine serum albumin (BSA; SEON(LA-BSA)), or with dextran (SEON(DEX)). Both micronuclei testing and the detection of ?H2A.X revealed no genotoxic effects of SEON(LA-BSA), SEON(DEX) or SEON(LA). Thus, it was demonstrated that different coatings of SPIONs improve biocompatibility, especially in terms of genotoxicity towards cells of the reproductive system.



FAU Authors / FAU Editors

Alexiou, Christoph Prof. Dr.
Professur für Nanomedizin
Janko, Christina Dr. rer. nat.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie
Unterweger, Harald Dr.-Ing.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie
Zaloga, Jan Dr. rer. nat.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie


Additional Organisation
Exzellenz-Cluster Engineering of Advanced Materials


External institutions
Ludwig-Maximilians-Universität (LMU)


How to cite

APA:
Poettler, M., Staicu, A., Zaloga, J., Unterweger, H., Weigel, B., Schreiber, E.,... Janko, C. (2015). Genotoxicity of Superparamagnetic Iron Oxide Nanoparticles in Granulosa Cells. International Journal of Molecular Sciences, 16(11), 26280-90. https://dx.doi.org/10.3390/ijms161125960

MLA:
Poettler, Marina, et al. "Genotoxicity of Superparamagnetic Iron Oxide Nanoparticles in Granulosa Cells." International Journal of Molecular Sciences 16.11 (2015): 26280-90.

BibTeX: 

Last updated on 2018-06-10 at 02:34