Shell matters: Magnetic targeting of SPIONs and in vitro effects on endothelial and monocytic cell function

Journal article


Publication Details

Author(s): Matuszak J, Doerfler P, Zaloga J, Unterweger H, Lyer S, Dietel B, Alexiou C, Cicha I
Journal: Clinical Hemorheology and Microcirculation
Publication year: 2015
Volume: 61
Journal issue: 2
Pages range: 259-77
ISSN: 1386-0291


Abstract


Superparamagnetic iron oxide nanoparticles (SPIONs) are versatile and easily functionalized agents with high potential for diagnostic and therapeutic intravascular applications. In this study, we analyzed the responses of endothelial (ECs) and monocytic cells to three different types of SPIONs, in order to assess the influence of physico-chemical properties on the biological reactions to SPIONs. The following formulations were used: (1) Lauric acid-coated and BSA-stabilized SPION-1,(2) Lauric acid/BSA-coated SPION-2 and (3) dextran-coated SPION-3. SPION-1 were strongly internalized by ECs and reduced their viability in static conditions. Additionally, they had a dose-dependent inhibitory effect on monocytic cell chemotaxis to MCP-1, but did not affect monocytic cell recruitment by ECs. SPION-2 uptake was less pronounced, both in ECs and monocytic cells, and these particles were better tolerated by the vascular cells. Not being internalized by endothelial or monocytic cells, SPION-3 did not induce relevant effects on cell viability, motility or endothelial-monocytic cell interactions.Taken together, localized accumulation of circulating SPION under physiologic-like flow conditions and their cellular uptake depends on the physicochemical characteristics. Our findings suggest that SPION-2 are suitable for magnetic targeting of atherosclerotic plaques. Due to their excellent biocompatibility and low internalization, SPION-3 may represent a suitable imaging agent for intravascular applications.



FAU Authors / FAU Editors

Alexiou, Christoph Prof. Dr.
Professur für Nanomedizin
Dietel, Barbara
Graduiertenzentrum der FAU
Lyer, Stefan Dr. rer. nat.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie
Matuszak, Jasmin
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie
Unterweger, Harald Dr.-Ing.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie
Zaloga, Jan Dr. rer. nat.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie


How to cite

APA:
Matuszak, J., Doerfler, P., Zaloga, J., Unterweger, H., Lyer, S., Dietel, B.,... Cicha, I. (2015). Shell matters: Magnetic targeting of SPIONs and in vitro effects on endothelial and monocytic cell function. Clinical Hemorheology and Microcirculation, 61(2), 259-77. https://dx.doi.org/10.3233/CH-151998

MLA:
Matuszak, Jasmin, et al. "Shell matters: Magnetic targeting of SPIONs and in vitro effects on endothelial and monocytic cell function." Clinical Hemorheology and Microcirculation 61.2 (2015): 259-77.

BibTeX: 

Last updated on 2019-18-07 at 07:36