IFN-α/IFN-λ responses to respiratory viruses in paediatric asthma

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autor(en): Bergauer A, Sopel N, Kross B, Vuorinen T, Xepapadaki P, Weiss ST, Blau A, Sharma H, Kraus C, Springel R, Rauh M, Mittler S, Graser A, Zimmermann T, Melichar VO, Kiefer A, Kowalski ML, Sobanska A, Jartti T, Lukkarinen H, Papadopoulos NG, Finotto S
Zeitschrift: European Respiratory Journal
Jahr der Veröffentlichung: 2016
ISSN: 0903-1936


Abstract


We analysed the influence of rhinovirus (RV) in nasopharyngeal fluid (NPF) on type I and III interferon (IFN) responses (e.g. IFN-? and IFN -: ?) and their signal transduction, at baseline and during disease exacerbation, in cohorts of pre-school children with and without asthma.At the time of recruitment into the Europe-wide study PreDicta, and during symptoms, NPF was collected and the local RV colonisation was analysed. Peripheral blood mononuclear cells (PBMCs) were challenged in vitro with RV or not. RNA was analysed by quantitative real-time PCR and gene arrays. Serum was analysed with ELISA for IFNs and C-reactive protein.We found that PBMCs from asthmatic children infected in vitro with the RV1b serotype upregulated MYD88, IRF1, STAT1 and STAT2 mRNA, whereas MYD88, IRF1, STAT1 and IRF9 were predominantly induced in control children. Moreover, during symptomatic visits because of disease exacerbation associated with RV detection in NPF, IFN-? production was found increased, while IFN-? secretion was already induced by RV in asthmatic children at baseline.During asthma exacerbations associated with RV, asthmatic children can induce IFN-? secretion, indicating a hyperactive immune response to repeated respiratory virus infection.



FAU-Autoren / FAU-Herausgeber

Bergauer, Annika
Professur für Molekulare Pneumologie
Graser, Anna Dr. rer. nat.
Molekular-Pneumologische Abteilung
Kiefer, Alexander
Lehrstuhl für Klinische Nuklearmedizin
Rauh, Manfred Prof. Dr.
Kinder- und Jugendklinik
Sopel, Nina Dr. rer. nat.
Molekular-Pneumologische Abteilung
Zimmermann, Theodor apl. Prof. Dr.
Medizinische Fakultät


Autor(en) der externen Einrichtung(en)
Medical University of Łódź / Uniwersytet Medyczny w Łodzi
National and Kapodistrian University of Athens
Partners HealthCare
Turku University Hospital / Turun yliopistollinen keskussairaala (TYKS)
University of Turku / Turun yliopisto


Zitierweisen

APA:
Bergauer, A., Sopel, N., Kross, B., Vuorinen, T., Xepapadaki, P., Weiss, S.T.,... Finotto, S. (2016). IFN-α/IFN-λ responses to respiratory viruses in paediatric asthma. European Respiratory Journal. https://dx.doi.org/10.1183/13993003.00969-2016

MLA:
Bergauer, Annika, et al. "IFN-α/IFN-λ responses to respiratory viruses in paediatric asthma." European Respiratory Journal (2016).

BibTeX: 

Zuletzt aktualisiert 2018-06-10 um 02:16