IFN-α/IFN-λ responses to respiratory viruses in paediatric asthma

Bergauer A, Sopel N, Kross B, Vuorinen T, Xepapadaki P, Weiss ST, Blau A, Sharma H, Kraus C, Springel R, Rauh M, Mittler S, Graser A, Zimmermann T, Melichar VO, Kiefer A, Kowalski ML, Sobanska A, Jartti T, Lukkarinen H, Papadopoulos NG, Finotto S (2016)

Publication Type: Journal article

Publication year: 2016

Journal

European Respiratory Journal European Respiratory Society

DOI: 10.1183/13993003.00969-2016

Abstract

We analysed the influence of rhinovirus (RV) in nasopharyngeal fluid (NPF) on type I and III interferon (IFN) responses (e.g. IFN-? and IFN -: ?) and their signal transduction, at baseline and during disease exacerbation, in cohorts of pre-school children with and without asthma.At the time of recruitment into the Europe-wide study PreDicta, and during symptoms, NPF was collected and the local RV colonisation was analysed. Peripheral blood mononuclear cells (PBMCs) were challenged in vitro with RV or not. RNA was analysed by quantitative real-time PCR and gene arrays. Serum was analysed with ELISA for IFNs and C-reactive protein.We found that PBMCs from asthmatic children infected in vitro with the RV1b serotype upregulated MYD88, IRF1, STAT1 and STAT2 mRNA, whereas MYD88, IRF1, STAT1 and IRF9 were predominantly induced in control children. Moreover, during symptomatic visits because of disease exacerbation associated with RV detection in NPF, IFN-? production was found increased, while IFN-? secretion was already induced by RV in asthmatic children at baseline.During asthma exacerbations associated with RV, asthmatic children can induce IFN-? secretion, indicating a hyperactive immune response to repeated respiratory virus infection.

Authors with CRIS profile

Annika Bergauer Professur für Molekulare Pneumologie Nina Sopel Department of Molecular Pneumology Manfred Rauh Department of Paediatrics and Adolescent Medicine Anna Graser Department of Molecular Pneumology Theodor Zimmermann Medizinische Fakultät Alexander Kiefer Lehrstuhl für Klinische Nuklearmedizin Susetta Neurath-Finotto Department of Molecular Pneumology

Involved external institutions

Partners HealthCare US United States (USA) (US) Turku University Hospital / Turun yliopistollinen keskussairaala (TYKS) FI Finland (FI) University of Turku / Turun yliopisto FI Finland (FI) National and Kapodistrian University of Athens GR Greece (GR) Medical University of Łódź / Uniwersytet Medyczny w Łodzi PL Poland (PL)

How to cite

APA:

Bergauer, A., Sopel, N., Kross, B., Vuorinen, T., Xepapadaki, P., Weiss, S.T.,... Finotto, S. (2016). IFN-α/IFN-λ responses to respiratory viruses in paediatric asthma. European Respiratory Journal. https://dx.doi.org/10.1183/13993003.00969-2016

MLA:

Bergauer, Annika, et al. "IFN-α/IFN-λ responses to respiratory viruses in paediatric asthma." European Respiratory Journal (2016).

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