Measuring fast stochastic displacements of bio-membranes with dynamic optical displacement spectroscopy

Beitrag in einer Fachzeitschrift
(Originalarbeit)


Details zur Publikation

Autor(en): Monzel C, Schmidt D, Kleusch C, Kirchenbuechler D, Seifert U, Smith AS, Sengupta K, Merkel R
Zeitschrift: Nature Communications
Verlag: NATURE PUBLISHING GROUP
Jahr der Veröffentlichung: 2015
Band: 6
ISSN: 2041-1723
Sprache: Englisch


Abstract


Stochastic displacements or fluctuations of biological membranes are increasingly recognized as an important aspect of many physiological processes, but hitherto their precise quantification in living cells was limited due to a lack of tools to accurately record them. Here we introduce a novel technique-dynamic optical displacement spectroscopy (DODS), to measure stochastic displacements of membranes with unprecedented combined spatio-temporal resolution of 20 nm and 10 mu s. The technique was validated by measuring bending fluctuations of model membranes. DODS was then used to explore the fluctuations in human red blood cells, which showed an ATP-induced enhancement of non-Gaussian behaviour. Plasma membrane fluctuations of human macrophages were quantified to this accuracy for the first time. Stimulation with a cytokine enhanced non-Gaussian contributions to these fluctuations. Simplicity of implementation, and high accuracy make DODS a promising tool for comprehensive understanding of stochastic membrane processes.



FAU-Autoren / FAU-Herausgeber

Schmidt, Daniel
Professur für Theoretische Physik
Smith, Ana-Suncana Prof. Dr.
Professur für Theoretische Physik


Zusätzliche Organisationseinheit(en)
Exzellenz-Cluster Engineering of Advanced Materials


Autor(en) der externen Einrichtung(en)
Aix-Marseille University / Aix-Marseille Université
Forschungszentrum Jülich GmbH (FZJ)
Universität Stuttgart


Zitierweisen

APA:
Monzel, C., Schmidt, D., Kleusch, C., Kirchenbuechler, D., Seifert, U., Smith, A.-S.,... Merkel, R. (2015). Measuring fast stochastic displacements of bio-membranes with dynamic optical displacement spectroscopy. Nature Communications, 6. https://dx.doi.org/10.1038/ncomms9162

MLA:
Monzel, Cornelia, et al. "Measuring fast stochastic displacements of bio-membranes with dynamic optical displacement spectroscopy." Nature Communications 6 (2015).

BibTeX: 

Zuletzt aktualisiert 2018-06-08 um 21:55