Mucosal expression of DEC-205 targeted allergen alleviates an asthmatic phenotype in mice
Maaske A, Devos FC, Niezold T, Lapuente D, Tannapfel A, Vanoirbeek JA, Überla K, Peters M, Tenbusch M (2016)
Publication Type: Journal article
Publication year: 2016
Journal
Book Volume: 237
Pages Range: 14-22
DOI: 10.1016/j.jconrel.2016.06.043
Abstract
Considering the rising incidence of allergic asthma, the symptomatic treatments that are currently applied in most cases are less than ideal. Specific immunotherapy is currently the only treatment that is able to change the course of the disease, but suffers from a long treatment duration. A gene based immunization that elicits the targeting of allergens towards dendritic cells in a steady-state environment might have the potential to amend these difficulties. Here we used a replication deficient adenovirus to induce the mucosal expression of OVA coupled to a single-chain antibody against DEC-205. A single intranasal vaccination was sufficient to mitigate an OVA-dependent asthmatic phenotype in a murine model. Invasive airway measurements demonstrated improved lung function after Ad-Dec-OVA treatment, which was in line with a marked reduction of goblet cell hyperplasia and lung eosinophilia. Furthermore OVA-specific IgE titers and production of type 2 cytokines were significantly reduced. Together, the here presented data demonstrate the feasibility of mucosal expression of DEC-targeted allergens as a treatment of allergic asthma.
Authors with CRIS profile
Klaus Überla
Lehrstuhl für Klinische und Molekulare Virologie
Matthias Tenbusch
Professur für Genbasierte Immunisierungsverfahren
Involved external institutions
Ruhr-Universität Bochum (RUB)
Germany (DE)
Katholieke Universiteit Leuven (KUL) / Catholic University of Leuven
Belgium (BE)
Germany (DE)
Katholieke Universiteit Leuven (KUL) / Catholic University of Leuven
Belgium (BE)
How to cite
APA:
Maaske, A., Devos, F.C., Niezold, T., Lapuente, D., Tannapfel, A., Vanoirbeek, J.A.,... Tenbusch, M. (2016). Mucosal expression of DEC-205 targeted allergen alleviates an asthmatic phenotype in mice. Journal of Controlled Release, 237, 14-22. https://doi.org/10.1016/j.jconrel.2016.06.043
MLA:
Maaske, A., et al. "Mucosal expression of DEC-205 targeted allergen alleviates an asthmatic phenotype in mice." Journal of Controlled Release 237 (2016): 14-22.
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