Identification of Sola l 4 as Bet v 1 homologous pathogenesis related-10 allergen in tomato fruits

Wangorsch A, Jamin A, Foetisch K, Malczyk A, Reuter A, Vierecke S, Schuelke S, Bartel D, Mahler V, Lidholm J, Vieths S, Scheurer S (2015)

Publication Type: Journal article

Publication year: 2015

Journal

Molecular Nutrition & Food Research Wiley-VCH Verlag

Book Volume: 59

Pages Range: 582-92

Journal Issue: 3

DOI: 10.1002/mnfr.201300620

Abstract

The aim was to investigate the potential contribution of a major birch pollen Bet v 1-homologous allergen to birch pollen-associated tomato fruit allergy.Two isoforms of a Bet v 1-homologous protein (designated Sola l 4) from tomato fruit were identified by cDNA-cloning and produced as recombinant proteins. Allergen-specific IgE levels to tomato, birch pollen, Bet v 1, and Sola l 4 were determined in birch pollen allergic patients with allergy or tolerance to tomato. Sola l 4 was recognized in 76% of birch/tomato allergic patients, while tomato- and Bet v 1-specific IgE was detectable in 64% and 81% of sera. Almost all patients sensitized to Bet v 1 reacted with Sola l 4. Both Sola l 4 isoforms displayed allergenic potency and IgE-cross-reactivity with Bet v 1 as investigated by competitive ELISA and in vitro mediator release assay. Nevertheless, the reactivity pattern of patients' sera was diverse.Sola l 4, a novel pathogenesis related-10 protein, qualifies as major allergen in tomato fruits. Data suggest Sola l 4 as class II allergen. IgE-testing using Sola l 4 showed low clinical specificity, but high sensitivity in tomato allergic patients and will further improve component-resolved allergy diagnosis.

Authors with CRIS profile

Vera Mahler Medizinische Fakultät

Additional Organisation(s)

Department of Dermatology

Involved external institutions

Paul Ehrlich Institute / Paul-Ehrlich-Institut – Bundesinstitut für Impfstoffe und biomedizinische Arzneimittel (PEI) DE Germany (DE) Thermo Fisher Scientific Inc. US United States (USA) (US)

How to cite

APA:

Wangorsch, A., Jamin, A., Foetisch, K., Malczyk, A., Reuter, A., Vierecke, S.,... Scheurer, S. (2015). Identification of Sola l 4 as Bet v 1 homologous pathogenesis related-10 allergen in tomato fruits. Molecular Nutrition & Food Research, 59(3), 582-92. https://dx.doi.org/10.1002/mnfr.201300620

MLA:

Wangorsch, Andrea, et al. "Identification of Sola l 4 as Bet v 1 homologous pathogenesis related-10 allergen in tomato fruits." Molecular Nutrition & Food Research 59.3 (2015): 582-92.

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