Inferring spatial organization of bonds within adhesion clusters by exploiting fluctuations of soft interfaces

Journal article
(Original article)


Publication Details

Author(s): Smith AS, Fenz SF, Sengupta K
Journal: EPL - Europhysics Letters
Publisher: EPL ASSOCIATION, EUROPEAN PHYSICAL SOCIETY
Publication year: 2010
Volume: 89
Journal issue: 2
ISSN: 0295-5075
Language: English


Abstract


Detecting the organization of bonds within adhesion domains connecting two interacting membranes is, at present, extremely challenging. Herein we present a technique, based on Reflection Interference Contrast Microscopy, which uses spontaneous thermal fluctuations of a soft interface as a tool to identify the organization of specific ligand-receptor bonds. The key is a time-resolved analysis of micro-interferometric data that systematically quantifies fluctuations and enables the detection of their suppression due to the formation of bonds, which, in turn, allows the identification of the bond organization without the use of fluorescent labelling. The identification of a new type of bond organization characterized by sparsely distributed bonds, as well as detection of pinning centres of nanometric size is presented. Copyright (C) EPLA, 2010



FAU Authors / FAU Editors

Smith, Ana-Suncana Prof. Dr.
Physics Underlying Life Science


Additional Organisation
Exzellenz-Cluster Engineering of Advanced Materials


External institutions with authors

Aix-Marseille University / Aix-Marseille Université
Forschungszentrum Jülich GmbH (FZJ)


Research Fields

A3 Multiscale Modeling and Simulation
Exzellenz-Cluster Engineering of Advanced Materials


How to cite

APA:
Smith, A.-S., Fenz, S.F., & Sengupta, K. (2010). Inferring spatial organization of bonds within adhesion clusters by exploiting fluctuations of soft interfaces. EPL - Europhysics Letters, 89(2). https://dx.doi.org/10.1209/0295-5075/89/28003

MLA:
Smith, Ana-Suncana, S. F. Fenz, and Kheya Sengupta. "Inferring spatial organization of bonds within adhesion clusters by exploiting fluctuations of soft interfaces." EPL - Europhysics Letters 89.2 (2010).

BibTeX: 

Last updated on 2018-06-08 at 21:32