Gabel LB, Kryschi C, Rusu CF (2006)
Publication Type: Journal article, Original article
Publication year: 2006
Original Authors: Comanici R., Gabel B., Gustavsson T., Markovitsi D., Cornaggia C., Pommeret S., Rusu C., Kryschi C.
Publisher: Elsevier
Book Volume: 325
Pages Range: 509-518
DOI: 10.1016/j.chemphys.2006.01.026
Photo-excited carminic acid and carminic acid-DNA complexes in a buffer solution at pH 7 have been examined using a variety of spectroscopy techniques, that are in particular, the femtosecond resolved fluorescence upconversion and transient absorption spectroscopy. The observation of dual fluorescence emission, one peaks at 470 nm and the other at 570 nm, indicates to an excited-state (S) intramolecular proton transfer (ESIPT). A detailed analysis of the transient absorption measurements of an aqueous carminic-acid solution at pH 7 yielded four lifetimes for the excited-state (S): 8, 15, 33 and 46 ps. On the other hand, only two lifetimes, 34 and 47 ps, were observed by fluorescence upconversion spectroscopy because of the detection limitation to the long wavelength edge of the carminic-acid spectrum. The four S lifetimes were ascribed to the coexistence of respectively two tautomer (normal and tautomer) forms of carminic acid, in the non-dissociated state (CAH) and in the deprotonated state (CA). The fluorescence upconversion measurements of carminic acid-DNA complexes exhibited a prolongation of the fluorescence lifetimes. This effect was accepted as evidence for the formation of intercalation complexes between the carminic acid and the DNA. The intercalative binding of the carminic acid to DNA was confirmed by the fluorescence titration experiments resulting to a binding constant of 2 × 10 M that is typical for anthracycline-DNA complexes. © 2006 Elsevier B.V. All rights reserved.
APA:
Gabel, L.-B., Kryschi, C., & Rusu, C.F. (2006). Femtosecond spectroscopic study of carminic acid-DNA interactions. Chemical Physics, 325, 509-518. https://doi.org/10.1016/j.chemphys.2006.01.026
MLA:
Gabel, Loredana-Bianca, Carola Kryschi, and Catalin Florin Rusu. "Femtosecond spectroscopic study of carminic acid-DNA interactions." Chemical Physics 325 (2006): 509-518.
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