CD83 Modulates B Cell Activation and Germinal Center Responses

Journal article


Publication Details

Author(s): Krzyzak L, Seitz C, Urbat A, Hutzler S, Ostalecki C, Glaesner J, Hiergeist A, Gessner A, Winkler T, Steinkasserer A, Nitschke L
Journal: Journal of Immunology
Publisher: AMER ASSOC IMMUNOLOGISTS
Publication year: 2016
Volume: 196
Journal issue: 9
Pages range: 3581-3594
ISSN: 0022-1767


Abstract


CD83 is a maturation marker for dendritic cells. In the B cell lineage, CD83 is expressed especially on activated B cells and on light zone B cells during the germinal center (GC) reaction. The function of CD83 during GC responses is unclear. CD83(-/-) mice have a strong reduction of CD4(+) T cells, which makes it difficult to analyze a functional role of CD83 on B cells during GC responses. Therefore, in the present study we generated a B cell-specific CD83 conditional knockout (CD83 B-cKO) model. CD83 B-cKO B cells show defective upregulation of MHC class II and CD86 expression and impaired proliferation after different stimuli. Analyses of GC responses after immunization with various Ags revealed a characteristic shift in dark zone and light zone B cell numbers, with an increase of B cells in the dark zone of CD83 B-cKO mice. This effect was not accompanied by alterations in the level of IgG immune responses or by major differences in affinity maturation. However, an enhanced IgE response was observed in CD83 B-cKO mice. Additionally, we observed a strong competitive disadvantage of CD83-cKO B cells in GC responses in mixed bone marrow chimeras. Furthermore, infection of mice with Borrelia burgdorferi revealed a defect in bacterial clearance of CD83 B-cKO mice with a shift toward a Th2 response, indicated by a strong increase in IgE titers. Taken together, our results show that CD83 is important for B cell activation and modulates GC composition and IgE Ab responses in vivo.



FAU Authors / FAU Editors

Krzyzak, Lena
Professur für Experimentelle Dermatologie
Nitschke, Lars Prof. Dr.
Professur für Genetik
Steinkasserer, Alexander Prof. Dr.
Professur für Experimentelle Dermatologie
Urbat, Anne
Professur für Genetik
Winkler, Thomas Prof. Dr.
Professur für Genetik


External institutions with authors

Universität Regensburg


How to cite

APA:
Krzyzak, L., Seitz, C., Urbat, A., Hutzler, S., Ostalecki, C., Glaesner, J.,... Nitschke, L. (2016). CD83 Modulates B Cell Activation and Germinal Center Responses. Journal of Immunology, 196(9), 3581-3594. https://dx.doi.org/10.4049/jimmunol.1502163

MLA:
Krzyzak, Lena, et al. "CD83 Modulates B Cell Activation and Germinal Center Responses." Journal of Immunology 196.9 (2016): 3581-3594.

BibTeX: 

Last updated on 2018-06-08 at 20:38