Generation of composites for bone tissue-engineering applications consisting of gellan gum hydrogels mineralized with calcium and magnesium phosphate phases by enzymatic means

Douglas TEL, Krawczyk G, Pamula E, Declercq HA, Schaubroeck D, Bucko MM, Balcaen L, Van Der Voort P, Bliznuk V, van den Vreken NMF, Dash M, Detsch R, Boccaccini AR, Vanhaecke F, Cornelissen M, Dubruel P (2014)

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2014

Journal

Journal of Tissue Engineering and Regenerative Medicine Wiley-Blackwell

Publisher: Wiley-Blackwell

Book Volume: 10

Pages Range: 938-954

Journal Issue: 11

DOI: 10.1002/term.1875

Abstract

Mineralization of hydrogels, desirable for bone regeneration applications, may be achieved enzymatically by incorporation of alkaline phosphatase (ALP). ALP-loaded gellan gum (GG) hydrogels were mineralized by incubation in mineralization media containing calcium and/or magnesium glycerophosphate (CaGP, MgGP). Mineralization media with CaGP:MgGP concentrations 0.1:0, 0.075:0.025, 0.05:0.05, 0.025:0.075 and 0:0.1 (all values mol/dm, denoted A, B, C, D and E, respectively) were compared. Mineral formation was confirmed by IR and Raman, SEM, ICP-OES, XRD, TEM, SAED, TGA and increases in the the mass fraction of the hydrogel not consisting of water. Ca was incorporated into mineral to a greater extent than Mg in samples mineralized in media A-D. Mg content and amorphicity of mineral formed increased in the order A<b<c<d. mineral="" formed="" in="" media="" a="" and="" b="" was="" calcium-deficient="" hydroxyapatite="" (cdha).="" medium="" c="" combination="" of="" cdha="" an="" amorphous="" phase.="" d="" e="" crystalline="" mgp.="" young's="" moduli="" storage="" decreased="" dependence="" mineralization="" the="" order="">B>C>D, but were significantly higher for samples mineralized in medium E. The attachment and vitality of osteoblastic MC3T3-E1 cells were higher on samples mineralized in media B-E (containing Mg) than in those mineralized in medium A (not containing Mg). All samples underwent degradation and supported the adhesion of RAW 264.7 monocytic cells, and samples mineralized in media A and B supported osteoclast-like cell formation. © 2014 John Wiley & Sons, Ltd.

Authors with CRIS profile

Rainer Detsch Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

Involved external institutions

Universiteit Gent (UGent) / Ghent University BE Belgium (BE) University of Science and Technology (AGH) / Akademia Górniczo-Hutnicza PL Poland (PL)

How to cite

APA:

Douglas, T.E.L., Krawczyk, G., Pamula, E., Declercq, H.A., Schaubroeck, D., Bucko, M.M.,... Dubruel, P. (2014). Generation of composites for bone tissue-engineering applications consisting of gellan gum hydrogels mineralized with calcium and magnesium phosphate phases by enzymatic means. Journal of Tissue Engineering and Regenerative Medicine, 10(11), 938-954. https://dx.doi.org/10.1002/term.1875

MLA:

Douglas, Timothy E L, et al. "Generation of composites for bone tissue-engineering applications consisting of gellan gum hydrogels mineralized with calcium and magnesium phosphate phases by enzymatic means." Journal of Tissue Engineering and Regenerative Medicine 10.11 (2014): 938-954.

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