Antibacterial 45S5 Bioglass®-based scaffolds reinforced with genipin cross-linked gelatin for bone tissue engineering

Journal article
(Original article)


Publication Details

Author(s): Li W, Wang H, Ding Y, Scheithauer EC, Goudouri OM, Grünewald A, Detsch R, Agarwal S, Boccaccini AR
Journal: Journal of Materials Chemistry B
Publication year: 2015
Volume: 3
Journal issue: 16
Pages range: 3367-3378
ISSN: 2050-750X
eISSN: 2050-7518


Abstract


45S5 Bioglass® (BG) scaffolds with high porosity (>90%) were coated with genipin cross-linked gelatin (GCG) and further incorporated with poly(p-xylyleneguanidine) hydrochloride (PPXG). The obtained GCG coated scaffolds maintained the high porosity and well interconnected pore structure. A 26-fold higher compressive strength was provided to 45S5 BG scaffolds by GCG coating, which slightly retarded but did not inhibit the in vitro bioactivity of 45S5 BG scaffolds in SBF. Moreover, the scaffolds were made antibacterial against both Gram-positive and Gram-negative bacteria by using polyguanidine, i.e. PPXG, in this study. Osteoblast-like cells (MG-63) were seeded onto PPXG and GCG coated scaffolds. PPXG was biocompatible with MG-63 cells at a low concentration (10 μg mL-1). MG-63 cells were shown to attach and spread on both uncoated and GCG coated scaffolds, and the mitochondrial activity measurement indicated that GCG coating had no negative influence on the cell proliferation behavior of MG-63 cells. The developed novel antibacterial bioactive 45S5 BG-based composite scaffolds with improved mechanical properties are promising candidates for bone tissue engineering.


FAU Authors / FAU Editors

Boccaccini, Aldo R. Prof. Dr.-Ing.
Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)
Detsch, Rainer Dr.-Ing.
Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)
Ding, Yaping
Lehrstuhl für Werkstoffwissenschaften (Polymerwerkstoffe)
Goudouri, Ourania Menti Dr.
Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)
Li, Wei
Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)


External institutions with authors

Universität Bayreuth


How to cite

APA:
Li, W., Wang, H., Ding, Y., Scheithauer, E.C., Goudouri, O.M., Grünewald, A.,... Boccaccini, A.R. (2015). Antibacterial 45S5 Bioglass®-based scaffolds reinforced with genipin cross-linked gelatin for bone tissue engineering. Journal of Materials Chemistry B, 3(16), 3367-3378. https://dx.doi.org/10.1039/c5tb00044k

MLA:
Li, Wei, et al. "Antibacterial 45S5 Bioglass®-based scaffolds reinforced with genipin cross-linked gelatin for bone tissue engineering." Journal of Materials Chemistry B 3.16 (2015): 3367-3378.

BibTeX: 

Last updated on 2019-27-06 at 10:17