Oxidative Burst-Dependent NETosis Is Implicated in the Resolution of Necrosis-Associated Sterile Inflammation

Journal article


Publication Details

Author(s): Biermann MHC, Podolska M, Knopf J, Reinwald C, Weidner D, Maueröder C, Hahn J, Kienhoefer D, Barras A, Boukherroub R, Szunerits S, Bilyy R, Hoffmann M, Zhao Y, Schett G, Herrmann M, Munoz Becerra LE
Journal: Frontiers in Immunology
Publication year: 2016
Volume: 7
Pages range: 557
ISSN: 1664-3224


Abstract


Necrosis is associated with a profound inflammatory response. The regulation of necrosis-associated inflammation, particularly the mechanisms responsible for resolution of inflammation is incompletely characterized. Nanoparticles are known to induce plasma membrane damage and necrosis followed by sterile inflammation. We observed that injection of metabolically inert nanodiamonds resulted in paw edema in WT and Ncf1** mice. However, while inflammation quickly resolved in WT mice, it persisted over several weeks in Ncf1** mice indicating failure of resolution of inflammation. Mechanistically, NOX2-dependent reactive oxygen species (ROS) production and formation of neutrophil extracellular traps were essential for the resolution of necrosis-induced inflammation: hence, by evaluating the fate of the particles at the site of inflammation, we observed that Ncf1** mice deficient in NADPH-dependent ROS failed to generate granulation tissue therefore being unable to trap the nanodiamonds. These data suggest that NOX2-dependent NETosis is crucial for preventing the chronification of the inflammatory response to tissue necrosis by forming NETosis-dependent barriers between the necrotic and healthy surrounding tissue.



FAU Authors / FAU Editors

Hahn, Jonas
Medizinische Klinik 3 - Rheumatologie und Immunologie
Herrmann, Martin Prof. Dr.
Medizinische Klinik 3 - Rheumatologie und Immunologie
Medizinische Klinik 3 - Rheumatologie und Immunologie
Knopf, Jasmin
Medizinische Klinik 3 - Rheumatologie und Immunologie
Maueröder, Christian
Medizinische Klinik 3 - Rheumatologie und Immunologie
Munoz Becerra, Luis Enrique Dr.
Medizinische Klinik 3 - Rheumatologie und Immunologie
Podolska, Malgorzata
Medizinische Klinik 3 - Rheumatologie und Immunologie
Reinwald, Christiane
Medizinische Klinik 3 - Rheumatologie und Immunologie
Schett, Georg Prof. Dr. med.
Lehrstuhl für Innere Medizin III


External institutions
Danylo Halytsky Lviv National Medical University / Львiвський Національний Медичний Унiверситет iм. Данила Галицького
LILLE 1 University - Science and Technology
Sichuan University (SCU) / 四川大学


How to cite

APA:
Biermann, M.H.C., Podolska, M., Knopf, J., Reinwald, C., Weidner, D., Maueröder, C.,... Munoz Becerra, L.E. (2016). Oxidative Burst-Dependent NETosis Is Implicated in the Resolution of Necrosis-Associated Sterile Inflammation. Frontiers in Immunology, 7, 557. https://dx.doi.org/10.3389/fimmu.2016.00557

MLA:
Biermann, Mona H. C., et al. "Oxidative Burst-Dependent NETosis Is Implicated in the Resolution of Necrosis-Associated Sterile Inflammation." Frontiers in Immunology 7 (2016): 557.

BibTeX: 

Last updated on 2019-17-04 at 08:17