Oxidative Burst-Dependent NETosis Is Implicated in the Resolution of Necrosis-Associated Sterile Inflammation

Biermann MHC, Podolska M, Knopf J, Reinwald C, Weidner D, Maueröder C, Hahn J, Kienhoefer D, Barras A, Boukherroub R, Szunerits S, Bilyy R, Hoffmann M, Zhao Y, Schett G, Herrmann M, Munoz Becerra L (2016)

Publication Type: Journal article

Publication year: 2016

Journal

Frontiers in Immunology Frontiers Research Foundation / Frontiers Media

Book Volume: 7

Pages Range: 557

DOI: 10.3389/fimmu.2016.00557

Abstract

Necrosis is associated with a profound inflammatory response. The regulation of necrosis-associated inflammation, particularly the mechanisms responsible for resolution of inflammation is incompletely characterized. Nanoparticles are known to induce plasma membrane damage and necrosis followed by sterile inflammation. We observed that injection of metabolically inert nanodiamonds resulted in paw edema in WT and Ncf1** mice. However, while inflammation quickly resolved in WT mice, it persisted over several weeks in Ncf1** mice indicating failure of resolution of inflammation. Mechanistically, NOX2-dependent reactive oxygen species (ROS) production and formation of neutrophil extracellular traps were essential for the resolution of necrosis-induced inflammation: hence, by evaluating the fate of the particles at the site of inflammation, we observed that Ncf1** mice deficient in NADPH-dependent ROS failed to generate granulation tissue therefore being unable to trap the nanodiamonds. These data suggest that NOX2-dependent NETosis is crucial for preventing the chronification of the inflammatory response to tissue necrosis by forming NETosis-dependent barriers between the necrotic and healthy surrounding tissue.

Authors with CRIS profile

Malgorzata Podolska Department of Medicine 3 – Rheumatology and Immunology Jasmin Knopf Department of Medicine 3 – Rheumatology and Immunology Christiane Reinwald Department of Medicine 3 – Rheumatology and Immunology Christian Maueröder Department of Medicine 3 – Rheumatology and Immunology Jonas Hahn Department of Medicine 3 – Rheumatology and Immunology Markus Hoffmann Department of Medicine 3 – Rheumatology and Immunology Georg Schett Lehrstuhl für Innere Medizin III Martin Herrmann Department of Medicine 3 – Rheumatology and Immunology Luis Munoz Becerra Department of Medicine 3 – Rheumatology and Immunology

Involved external institutions

Sichuan University (SCU) / 四川大学 CN China (CN) Danylo Halytsky Lviv National Medical University / Львiвський Національний Медичний Унiверситет iм. Данила Галицького UA Ukraine (UA) LILLE 1 University - Science and Technology FR France (FR)

How to cite

APA:

Biermann, M.H.C., Podolska, M., Knopf, J., Reinwald, C., Weidner, D., Maueröder, C.,... Munoz Becerra, L. (2016). Oxidative Burst-Dependent NETosis Is Implicated in the Resolution of Necrosis-Associated Sterile Inflammation. Frontiers in Immunology, 7, 557. https://dx.doi.org/10.3389/fimmu.2016.00557

MLA:

Biermann, Mona H. C., et al. "Oxidative Burst-Dependent NETosis Is Implicated in the Resolution of Necrosis-Associated Sterile Inflammation." Frontiers in Immunology 7 (2016): 557.

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