Optimal translational termination requires C4 lysyl hydroxylation of eRF1

Feng T, Yamamoto A, Wilkins SE, Sokolova E, Yates LA, Muenzel M, Singh P, Hopkinson RJ, Fischer R, Cockman ME, Shelley J, Trudgian DC, Schoedel J, Mccullagh JSO, Ge W, Kessler BM, Gilbert RJ, Frolova LY, Alkalaeva E, Ratcliffe PJ, Schofield CJ, Coleman ML (2014)

Publication Type: Journal article

Publication year: 2014

Journal

Molecular Cell Elsevier (Cell Press)

Book Volume: 53

Pages Range: 645-54

Journal Issue: 4

DOI: 10.1016/j.molcel.2013.12.028

Abstract

Efficient stop codon recognition and peptidyl-tRNA hydrolysis are essential in order to terminate translational elongation and maintain protein sequence fidelity. Eukaryotic translational termination is mediated by a release factor complex that includes eukaryotic release factor 1 (eRF1) and eRF3. The N terminus of eRF1 contains highly conserved sequence motifs that couple stop codon recognition at the ribosomal A site to peptidyl-tRNA hydrolysis. We reveal that Jumonji domain-containing 4 (Jmjd4), a 2-oxoglutarate- and Fe(II)-dependent oxygenase, catalyzes carbon 4 (C4) lysyl hydroxylation of eRF1. This posttranslational modification takes place at an invariant lysine within the eRF1 NIKS motif and is required for optimal translational termination efficiency. These findings further highlight the role of 2-oxoglutarate/Fe(II) oxygenases in fundamental cellular processes and provide additional evidence that ensuring fidelity of protein translation is a major role of hydroxylation.

Involved external institutions

University of Oxford GB United Kingdom (GB) Russian Academy of Sciences / Росси́йская акаде́мия нау́к (RAS) RU Russian Federation (RU) University of Texas Southwestern Medical Center (UT Southwestern) US United States (USA) (US)

How to cite

APA:

Feng, T., Yamamoto, A., Wilkins, S.E., Sokolova, E., Yates, L.A., Muenzel, M.,... Coleman, M.L. (2014). Optimal translational termination requires C4 lysyl hydroxylation of eRF1. Molecular Cell, 53(4), 645-54. https://dx.doi.org/10.1016/j.molcel.2013.12.028

MLA:

Feng, Tianshu, et al. "Optimal translational termination requires C4 lysyl hydroxylation of eRF1." Molecular Cell 53.4 (2014): 645-54.

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