Circulating miR-148b and miR-133a as biomarkers for breast cancer detection

Shen J, Hu Q, Schrauder M, Yan L, Wang D, Medico L, Guo Y, Yao S, Zhu Q, Liu B, Qin M, Beckmann M, Fasching P, Strick R, Johnson CS, Ambrosone CB, Zhao H, Liu S (2014)

Publication Type: Journal article

Publication year: 2014

Journal

Oncotarget Impact Journals

Book Volume: 5

Pages Range: 5284-94

Journal Issue: 14

DOI: 10.18632/oncotarget.2014

Abstract

Circulating microRNAs have drawn a great deal of attention as promising novel biomarkers for breast cancer. However, to date, the results are mixed. Here, we performed a three-stage microRNA analysis using plasma samples from breast cancer patients and healthy controls, with efforts taken to address several pitfalls in detection techniques and study design observed in previous studies. In the discovery phase with 122 Caucasian study subjects, we identified 43 microRNAs differentially expressed between breast cancer cases and healthy controls. When those microRNAs were compared with published data from other studies, we identified three microRNAs, including miR-148b, miR-133a and miR-409-3p, whose plasma levels were significantly higher in breast cancer cases than healthy controls and were also significant in previous independent studies. In the validation phase with 50 breast cancer cases and 50 healthy controls, we validated the associations with breast cancer detection for miR-148b and miR-133a (P = 1.5×10-6 and 1.3×10-10, respectively). In the in-vitro study phase, we found that both miR-148b and miR-133a were secreted from breast cancer cell lines, showing their secretory potential and possible tumor origin. Thus, our data suggest that both miR-148b and miR-133a have potential use as biomarkers for breast cancer detection.

Authors with CRIS profile

Matthias Beckmann Lehrstuhl für Geburtshilfe und Frauenheilkunde Peter Fasching Professur für Translationale Frauenheilkunde und Geburtshilfe Reiner Strick Medizinische Fakultät

Involved external institutions

Roswell Park Cancer Institute US United States (USA) (US) University of Texas MD Anderson Cancer Center US United States (USA) (US)

How to cite

APA:

Shen, J., Hu, Q., Schrauder, M., Yan, L., Wang, D., Medico, L.,... Liu, S. (2014). Circulating miR-148b and miR-133a as biomarkers for breast cancer detection. Oncotarget, 5(14), 5284-94. https://doi.org/10.18632/oncotarget.2014

MLA:

Shen, Jie, et al. "Circulating miR-148b and miR-133a as biomarkers for breast cancer detection." Oncotarget 5.14 (2014): 5284-94.

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