Traction Force Microscopy in 3-Dimensional Extracellular Matrix Networks.

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autorinnen und Autoren: Cóndor M, Steinwachs J, Mark C, Garcia-Aznar JM, Fabry B
Zeitschrift: Current protocols in cell biology
Jahr der Veröffentlichung: 2017
Band: 75
Seitenbereich: 10.22.1-10.22.20
ISSN: 1934-2616


Abstract


Cell migration through a three-dimensional (3-D) matrix depends strongly on the ability of cells to generate traction forces. To overcome the steric hindrance of the matrix, cells need to generate sufficiently high traction forces but also need to distribute these forces spatially in a migration-promoting way. This unit describes a protocol to measure spatial maps of cell traction forces in 3-D biopolymer networks such as collagen, fibrin, or Matrigel. Traction forces are computed from the relationship between measured force-induced matrix deformations surrounding the cell and the known mechanical properties of the matrix. The method does not rely on knowledge of the cell surface coordinates and takes nonlinear mechanical properties of the matrix into account. © 2017 by John Wiley & Sons, Inc.



FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Fabry, Ben Prof. Dr.
Lehrstuhl für Biophysik
Mark, Christoph Dr.
Lehrstuhl für Biophysik
Steinwachs, Julian
Lehrstuhl für Biophysik


Einrichtungen weiterer Autorinnen und Autoren

Universidad de Zaragoza


Zitierweisen

APA:
Cóndor, M., Steinwachs, J., Mark, C., Garcia-Aznar, J.M., & Fabry, B. (2017). Traction Force Microscopy in 3-Dimensional Extracellular Matrix Networks. Current protocols in cell biology, 75, 10.22.1-10.22.20. https://dx.doi.org/10.1002/cpcb.24

MLA:
Cóndor, Mar, et al. "Traction Force Microscopy in 3-Dimensional Extracellular Matrix Networks." Current protocols in cell biology 75 (2017): 10.22.1-10.22.20.

BibTeX: 

Zuletzt aktualisiert 2018-10-08 um 22:57