Traction Force Microscopy in 3-Dimensional Extracellular Matrix Networks.

Journal article


Publication Details

Author(s): Cóndor M, Steinwachs J, Mark C, Garcia-Aznar JM, Fabry B
Journal: Current protocols in cell biology
Publication year: 2017
Volume: 75
Pages range: 10.22.1-10.22.20
ISSN: 1934-2616


Abstract


Cell migration through a three-dimensional (3-D) matrix depends strongly on the ability of cells to generate traction forces. To overcome the steric hindrance of the matrix, cells need to generate sufficiently high traction forces but also need to distribute these forces spatially in a migration-promoting way. This unit describes a protocol to measure spatial maps of cell traction forces in 3-D biopolymer networks such as collagen, fibrin, or Matrigel. Traction forces are computed from the relationship between measured force-induced matrix deformations surrounding the cell and the known mechanical properties of the matrix. The method does not rely on knowledge of the cell surface coordinates and takes nonlinear mechanical properties of the matrix into account. © 2017 by John Wiley & Sons, Inc.



FAU Authors / FAU Editors

Fabry, Ben Prof. Dr.
Lehrstuhl für Biophysik
Mark, Christoph Dr.
Lehrstuhl für Biophysik
Steinwachs, Julian
Lehrstuhl für Biophysik


External institutions with authors

Universidad de Zaragoza


How to cite

APA:
Cóndor, M., Steinwachs, J., Mark, C., Garcia-Aznar, J.M., & Fabry, B. (2017). Traction Force Microscopy in 3-Dimensional Extracellular Matrix Networks. Current protocols in cell biology, 75, 10.22.1-10.22.20. https://dx.doi.org/10.1002/cpcb.24

MLA:
Cóndor, Mar, et al. "Traction Force Microscopy in 3-Dimensional Extracellular Matrix Networks." Current protocols in cell biology 75 (2017): 10.22.1-10.22.20.

BibTeX: 

Last updated on 2018-10-08 at 22:57