DNA-Dye-Conjugates: Conformations and Spectra of Fluorescence Probes

Beierlein F, Paradas Palomo M, Sharapa D, Zozulia O, Mokhir A, Clark T (2016)


Publication Type: Journal article

Publication year: 2016

Journal

Book Volume: 11

Pages Range: e0160229

Journal Issue: 7

DOI: 10.1371/journal.pone.0160229

Abstract

Extensive molecular-dynamics (MD) simulations have been used to investigate DNA-dye and DNA-photosensitizer conjugates, which act as reactants in templated reactions leading to the generation of fluorescent products in the presence of specific desoxyribonucleic acid sequences (targets). Such reactions are potentially suitable for detecting target nucleic acids in live cells by fluorescence microscopy or flow cytometry. The simulations show how the attached dyes/photosensitizers influence DNA structure and reveal the relative orientations of the chromophores with respect to each other. Our results will help to optimize the reactants for the templated reactions, especially length and structure of the spacers used to link reporter dyes or photosensitizers to the oligonucleotides responsible for target recognition. Furthermore, we demonstrate that the structural ensembles obtained from the simulations can be used to calculate steady-state UV-vis absorption and emission spectra. We also show how important quantities describing the quenching of the reporter dye via fluorescence resonance energy transfer (FRET) can be calculated from the simulation data, and we compare these for different relative chromophore geometries.

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APA:

Beierlein, F., Paradas Palomo, M., Sharapa, D., Zozulia, O., Mokhir, A., & Clark, T. (2016). DNA-Dye-Conjugates: Conformations and Spectra of Fluorescence Probes. Plos One, 11(7), e0160229. https://dx.doi.org/10.1371/journal.pone.0160229

MLA:

Beierlein, Frank, et al. "DNA-Dye-Conjugates: Conformations and Spectra of Fluorescence Probes." Plos One 11.7 (2016): e0160229.

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