Molecular characterization of a calmodulin involved in the signal transduction chain of gravitaxis in Euglena gracilis

Journal article


Publication Details

Author(s): Daiker V, Lebert M, Richter P, Häder DP
Journal: Planta
Publisher: Springer Verlag (Germany)
Publication year: 2010
Volume: 231
Journal issue: 5
Pages range: 1229-1236
ISSN: 0032-0935


Abstract


The unicellular flagellate Euglena gracilis shows a negative gravitactic behavior. This is based on physiological mechanisms which in the past have been indirectly assessed. Meanwhile, it was possible to isolate genes involved in the signal transduction chain of gravitaxis. The DNA sequences of five calmodulins were found in Euglena, one of which was only known in its protein structure (CaM.1); the other four are new. The biosynthesis of the corresponding proteins of CaM.1-CaM.5 was inhibited by means of RNA interference to determine their involvement in the gravitactic signal transduction chain. RNAi of CaM.1 inhibits free swimming of the cells and pronounced cell-form aberrations. The division of cells was also hampered. After recovery from RNAi the cell showed precise negative gravitaxis again. Blockage of CaM.3 to CaM. 5 did not impair gravitaxis. In contrast, the blockage of CaM.2 has only a transient and not pronounced influence on motility and cell form, but leads to a total loss of gravitactic orientation for more than 30 days. This indicates that CaM.2 is an element in the signal transduction chain of gravitaxis in E. gracilis. The results are discussed with regard to the current working model of gravitaxis in E. gracilis.



FAU Authors / FAU Editors

Daiker, Viktor Dr.
Lehrstuhl für Zellbiologie
Häder, Donat-Peter Prof. Dr.
Naturwissenschaftliche Fakultät
Lebert, Michael PD Dr.
Lehrstuhl für Zellbiologie
Richter, Peter Dr.
Lehrstuhl für Zellbiologie


How to cite

APA:
Daiker, V., Lebert, M., Richter, P., & Häder, D.-P. (2010). Molecular characterization of a calmodulin involved in the signal transduction chain of gravitaxis in Euglena gracilis. Planta, 231(5), 1229-1236. https://dx.doi.org/10.1007/s00425-010-1126-9

MLA:
Daiker, Viktor, et al. "Molecular characterization of a calmodulin involved in the signal transduction chain of gravitaxis in Euglena gracilis." Planta 231.5 (2010): 1229-1236.

BibTeX: 

Last updated on 2018-05-06 at 05:23