KORRIGAN, an arabidopsis endo-1,4-beta-glucanase, localizes to the cell plate by polarized targeting and is essential for cytokinesis

Journal article


Publication Details

Author(s): Zuo J, Niu QW, Nishizawa N, Wu Y, Kost B, Chua NH
Journal: Plant Cell
Publisher: American Society of Plant Biologists
Publication year: 2000
Volume: 12
Journal issue: 7
Pages range: 1137-1152
ISSN: 1040-4651


Abstract


The formation of the cell plate, a unique structure in dividing plant cells, is pivotal for cytokinesis, A mutation in the Arabidopsis KORRIGAN (KOR) gene causes the formation of aberrant cell plates, incomplete cell walls, and multinucleated cells, leading to severely abnormal seedling morphology. The mutant, designed kor1-2, was identified as a stronger allele than the previously identified kor1-1, which appears to be defective only in cell elongation. KOR1 encodes an endo-1,4-beta-D-glucanase with a transmembrane domain and two putative polarized targeting signals in the cytosolic tail. When expressed in tobacco BY2 cells, a KOR1-GFP (green fluorescence protein) fusion protein was localized to growing cell plates. Substitution mutations in the polarized targeting motifs of KOR1 caused the fusion proteins to localize to the plasma membrane as well. Expression of these mutant genes in kor1-2 plants complemented only the cell elongation defect but not the cytokinesis defect, indicating that polarized targeting of KOR1 to forming cell plates is essential for cytokinesis. Our results suggest that KOR1 plays a critical role during cytokinesis.



How to cite

APA:
Zuo, J., Niu, Q.-W., Nishizawa, N., Wu, Y., Kost, B., & Chua, N.-H. (2000). KORRIGAN, an arabidopsis endo-1,4-beta-glucanase, localizes to the cell plate by polarized targeting and is essential for cytokinesis. Plant Cell, 12(7), 1137-1152. https://dx.doi.org/10.1105/tpc.12.7.1137

MLA:
Zuo, Jianru, et al. "KORRIGAN, an arabidopsis endo-1,4-beta-glucanase, localizes to the cell plate by polarized targeting and is essential for cytokinesis." Plant Cell 12.7 (2000): 1137-1152.

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Last updated on 2018-05-06 at 01:10