Structural insight into the giant Ca²?-binding adhesin SiiE: implications for the adhesion of Salmonella enterica to polarized epithelial cells

Grießl M, Schmid B, Kaßler K, Braunsmann C, Ritter R, Barlag B, Stierhof YD, Sturm KU, Danzer CA, Wagner C, Schaeffer TE, Sticht H, Hensel M, Muller Y (2013)

Publication Type: Journal article

Publication year: 2013

Journal

Structure Elsevier (Cell Press)

Publisher: Elsevier (Cell Press)

Book Volume: 21

Pages Range: 741-52

Journal Issue: 5

DOI: 10.1016/j.str.2013.02.020

Abstract

SiiE from Salmonella enterica is a giant 5,559-residue-long nonfimbrial adhesin that is secreted by a type 1 secretion system (T1SS) and initiates bacterial adhesion to polarized host cells. Structural insight has been gained into the 53 bacterial Ig-like (BIg) domains of SiiE, which account for 94% of the entire SiiE sequence. The crystal structure of a fragment comprising BIg domains 50 to 52 of SiiE reveals the BIg domain architecture and highlights two types of SiiE-specific Ca²?-binding sites. Sequence homology considerations suggest that full-length SiiE interacts with more than 100 Ca²? ions. Molecular dynamics simulations and single-molecule imaging indicate that Ca²? binding confers SiiE with a rigid 200 nm rod-like habitus that is required to reach out beyond the Salmonella lipopolysaccharide layer and to promote adhesion to host cells. The crystal structure suggests plausible routes for the establishment of the initial contact between Salmonella and host cells.

Authors with CRIS profile

Martin Grießl Lehrstuhl für Biotechnik (Proteinstruktur und -design) Benedikt Schmid Lehrstuhl für Biotechnik (Proteinstruktur und -design) Kristin Kaßler Professur für Bioinformatik Claudia Agnes Danzer Professur für Medizinische Mikrobiologie und Infektionsimmunologie Heinrich Sticht Professur für Bioinformatik Yves Muller Lehrstuhl für Biotechnik (Proteinstruktur und -design)

Involved external institutions

Eberhard Karls Universität Tübingen DE Germany (DE) Universität Osnabrück DE Germany (DE)

How to cite

APA:

Grießl, M., Schmid, B., Kaßler, K., Braunsmann, C., Ritter, R., Barlag, B.,... Muller, Y. (2013). Structural insight into the giant Ca²?-binding adhesin SiiE: implications for the adhesion of Salmonella enterica to polarized epithelial cells. Structure, 21(5), 741-52. https://dx.doi.org/10.1016/j.str.2013.02.020

MLA:

Grießl, Martin, et al. "Structural insight into the giant Ca²?-binding adhesin SiiE: implications for the adhesion of Salmonella enterica to polarized epithelial cells." Structure 21.5 (2013): 741-52.

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