Structural insight into the giant Ca²?-binding adhesin SiiE: implications for the adhesion of Salmonella enterica to polarized epithelial cells

Journal article


Publication Details

Author(s): Grießl M, Schmid B, Kaßler K, Braunsmann C, Ritter R, Barlag B, Stierhof YD, Sturm KU, Danzer CA, Wagner C, Schaeffer TE, Sticht H, Hensel M, Muller Y
Journal: Structure
Publisher: Elsevier (Cell Press)
Publication year: 2013
Volume: 21
Journal issue: 5
Pages range: 741-52
ISSN: 0969-2126


Abstract


SiiE from Salmonella enterica is a giant 5,559-residue-long nonfimbrial adhesin that is secreted by a type 1 secretion system (T1SS) and initiates bacterial adhesion to polarized host cells. Structural insight has been gained into the 53 bacterial Ig-like (BIg) domains of SiiE, which account for 94% of the entire SiiE sequence. The crystal structure of a fragment comprising BIg domains 50 to 52 of SiiE reveals the BIg domain architecture and highlights two types of SiiE-specific Ca²?-binding sites. Sequence homology considerations suggest that full-length SiiE interacts with more than 100 Ca²? ions. Molecular dynamics simulations and single-molecule imaging indicate that Ca²? binding confers SiiE with a rigid 200 nm rod-like habitus that is required to reach out beyond the Salmonella lipopolysaccharide layer and to promote adhesion to host cells. The crystal structure suggests plausible routes for the establishment of the initial contact between Salmonella and host cells.



FAU Authors / FAU Editors

Danzer, Claudia Agnes
Professur für Medizinische Mikrobiologie und Infektionsimmunologie
Grießl, Martin Dr.
Lehrstuhl für Biotechnik (Proteinstruktur und -design)
Kaßler, Kristin
Professur für Bioinformatik
Muller, Yves Prof. Dr.
Lehrstuhl für Biotechnik (Proteinstruktur und -design)
Schmid, Benedikt Dr.
Lehrstuhl für Biotechnik (Proteinstruktur und -design)
Sticht, Heinrich Prof. Dr.
Professur für Bioinformatik


External institutions
Eberhard Karls Universität Tübingen
Universität Osnabrück


How to cite

APA:
Grießl, M., Schmid, B., Kaßler, K., Braunsmann, C., Ritter, R., Barlag, B.,... Muller, Y. (2013). Structural insight into the giant Ca²?-binding adhesin SiiE: implications for the adhesion of Salmonella enterica to polarized epithelial cells. Structure, 21(5), 741-52. https://dx.doi.org/10.1016/j.str.2013.02.020

MLA:
Grießl, Martin, et al. "Structural insight into the giant Ca²?-binding adhesin SiiE: implications for the adhesion of Salmonella enterica to polarized epithelial cells." Structure 21.5 (2013): 741-52.

BibTeX: 

Last updated on 2018-11-08 at 00:39