Prospero-related homeobox 1 gene (Prox1) is regulated by canonical Wnt signaling and has a stage-specific role in adult hippocampal neurogenesis

Karalay O, Doberauer K, Vadodaria KC, Knobloch M, Berti L, Miquelajauregui A, Schwark M, Jagasia R, Taketo MM, Tarabykin V, Lie DC, Jessberger S (2011)

Publication Type: Journal article

Publication year: 2011

Journal

Proceedings of the National Academy of Sciences of the United States of America National Academy of Sciences

Publisher: National Academy of Sciences

Book Volume: 108

Pages Range: 5807-12

Journal Issue: 14

DOI: 10.1073/pnas.1013456108

Abstract

Neural stem cells (NSCs) generate new granule cells throughout life in the mammalian hippocampus. Canonical Wnt signaling regulates the differentiation of NSCs towards the neuronal lineage. Here we identified the prospero-related homeodomain transcription factor Prox1 as a target of ?-catenin-TCF/LEF signaling in vitro and in vivo. Prox1 overexpression enhanced neuronal differentiation whereas shRNA-mediated knockdown of Prox1 impaired the generation of neurons in vitro and within the hippocampal niche. In contrast, Prox1 was not required for survival of adult-generated granule cells after they had matured, suggesting a role for Prox1 in initial granule cell differentiation but not in the maintenance of mature granule cells. The data presented here characterize a molecular pathway from Wnt signaling to a transcriptional target leading to granule cell differentiation within the adult brain and identify a stage-specific function for Prox1 in the process of adult neurogenesis.

Authors with CRIS profile

Dieter Chichung Lie Professur für Molekulare Medizin mit dem Schwerpunkt Molekulare Bildgebung

Involved external institutions

Eidgenössische Technische Hochschule Zürich (ETHZ) / Swiss Federal Institute of Technology in Zurich CH Switzerland (CH) Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich DE Germany (DE) Max-Planck-Institut für Experimentelle Medizin DE Germany (DE) Kyoto University / 京都大学 Kyōto daigaku JP Japan (JP)

How to cite

APA:

Karalay, O., Doberauer, K., Vadodaria, K.C., Knobloch, M., Berti, L., Miquelajauregui, A.,... Jessberger, S. (2011). Prospero-related homeobox 1 gene (Prox1) is regulated by canonical Wnt signaling and has a stage-specific role in adult hippocampal neurogenesis. Proceedings of the National Academy of Sciences of the United States of America, 108(14), 5807-12. https://doi.org/10.1073/pnas.1013456108

MLA:

Karalay, Oezlem, et al. "Prospero-related homeobox 1 gene (Prox1) is regulated by canonical Wnt signaling and has a stage-specific role in adult hippocampal neurogenesis." Proceedings of the National Academy of Sciences of the United States of America 108.14 (2011): 5807-12.

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