Alpha-synuclein activates BV2 microglia dependent on its aggregation state

Journal article


Publication Details

Author(s): Hoffmann A, Ettle B, Bruno A, Kulinich A, Hoffmann AC, von Wittgenstein J, Winkler J, Xiang W, Schlachetzki J
Journal: Biochemical and Biophysical Research Communications
Publisher: Elsevier
Publication year: 2016
Volume: 479
Journal issue: 4
Pages range: 881-886
ISSN: 0006-291X


Abstract


Synucleinopathies such as Parkinson's disease (PD), dementia with Lewy bodies (DLB), and multiple system atrophy (MSA) are defined by the presence of intracellular alpha-synuclein aggregates in neurons and/or oligodendrocytes. In addition, post mortem tissue analysis revealed profound changes in microglial morphology, indicating microglial activation and neuroinflammation. Thus, alpha-synuclein may directly activate microglia, leading to increased production of key pro-inflammatory cytokines like tumor necrosis factor-alpha (TNF-?) and interleukin-1beta (IL-1?), which in turn modulates the disease progression. The distinct alpha-synuclein species, which mediates the activation of microglia, is not well defined. We hypothesized that microglial activation depends on a specific aggregation state of alpha-synuclein. Here, we show that primarily human fibrillar alpha-synuclein increased the production and secretion of pro-inflammatory cytokines by microglial BV2 cells compared to monomeric and oligomeric alpha-synuclein. BV2 cells also preferentially phagocytosed fibrillar alpha-synuclein compared to alpha-synuclein monomers and oligomers. Microglial uptake of alpha-synuclein fibrils and the consequent activation were time- and concentration-dependent. Moreover, the degree of fibrillization determined the efficiency of microglial internalization. Taken together, our study highlights the specific crosstalk of distinct alpha-synuclein species with microglial cells.



FAU Authors / FAU Editors

Bruno, Ariane
Lehrstuhl für Biochemie und Molekulare Medizin
Ettle, Benjamin
Professur für Molekulare Neurologie
Hoffmann, Anna-Carin
Interdisziplinäres Zentrum für Klinische Forschung IZKF Prof.Dr.Kalden Medizinische Klinik 3
Hoffmann, Alana
Professur für Molekulare Neurologie
Schlachetzki, Johannes Dr. med.
Molekular-Neurologische Abteilung in der Neurologischen Klinik
von Wittgenstein, Julia
Professur für Molekulare Medizin mit dem Schwerpunkt Molekulare Bildgebung
Winkler, Jürgen Prof. Dr.
Molekular-Neurologische Abteilung in der Neurologischen Klinik
Xiang, Wei PD Dr.
Lehrstuhl für Biochemie und Molekulare Medizin


External institutions with authors

University of California, Riverside (UCR)


How to cite

APA:
Hoffmann, A., Ettle, B., Bruno, A., Kulinich, A., Hoffmann, A.-C., von Wittgenstein, J.,... Schlachetzki, J. (2016). Alpha-synuclein activates BV2 microglia dependent on its aggregation state. Biochemical and Biophysical Research Communications, 479(4), 881-886. https://dx.doi.org/10.1016/j.bbrc.2016.09.109

MLA:
Hoffmann, Alana, et al. "Alpha-synuclein activates BV2 microglia dependent on its aggregation state." Biochemical and Biophysical Research Communications 479.4 (2016): 881-886.

BibTeX: 

Last updated on 2018-05-10 at 02:28

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