Tracking micro-optical resonances for identifying and sensing novel procaspase-3 protein marker released from cell cultures in response to toxins

Journal article


Publication Details

Author(s): Chen YJ, Xiang W, Klucken J, Vollmer F, Klucken J
Journal: Nanotechnology
Publisher: Institute of Physics: Hybrid Open Access
Publication year: 2016
Volume: 27
Journal issue: 16
Pages range: 164001
ISSN: 0957-4484


Abstract


The response of cells to toxins is commonly investigated by detecting intracellular markers for cell death, such as caspase proteins. This requires the introduction of labels by the permeabilization or complete lysis of cells. Here we introduce a non-invasive tool for monitoring a caspase protein in the extracellular medium. The tool is based on highly sensitive optical micro-devices, referred to as whispering-gallery mode biosensors (WGMBs). WGMBs are functionalized with antibodies for the specific and label-free detection of procaspase-3 released from human embryonic kidney HEK293 and neuroglioma H4 cells after introducing staurosporine and rotenone toxins, respectively. Additional tests show that the extracellular accumulation of procaspase-3 is concomitant with a decrease in cell viability. The hitherto unknown release of procaspase-3 from cells in response to toxins and its accumulation in the medium is further investigated by Western blot, showing that the extracellular detection of procaspase-3 is interrelated with cytotoxicity of alpha-synuclein protein (aSyn) overexpressed in H4 cells. These studies provide evidence for procaspase-3 as a novel extracellular biomarker for cell death, with applications in cytotoxicity tests. Such WGMBs could be applied to further identify as-yet unknown extracellular biomarkers using established antibodies against intracellular antigens.



FAU Authors / FAU Editors

Klucken, Jochen Prof. Dr.
Medizinische Fakultät
Klucken, Jochen Prof. Dr.
Molekular-Neurologische Abteilung in der Neurologischen Klinik
Xiang, Wei PD Dr.
Lehrstuhl für Biochemie und Molekulare Medizin


External institutions with authors

Max-Planck-Institut für die Physik des Lichts (MPL) / Max Planck Institute for the Science of Light


How to cite

APA:
Chen, Y.-J., Xiang, W., Klucken, J., Vollmer, F., & Klucken, J. (2016). Tracking micro-optical resonances for identifying and sensing novel procaspase-3 protein marker released from cell cultures in response to toxins. Nanotechnology, 27(16), 164001. https://dx.doi.org/10.1088/0957-4484/27/16/164001

MLA:
Chen, Ying-Jen, et al. "Tracking micro-optical resonances for identifying and sensing novel procaspase-3 protein marker released from cell cultures in response to toxins." Nanotechnology 27.16 (2016): 164001.

BibTeX: 

Last updated on 2018-05-10 at 02:28