"Bubble-in-the-Roll" Technique Using the Endoject DMEK Injector: Influence of the Air Bubble on Endothelial Cell Loss
Akbaba Y, Weller JM, Rössler K, Armitage WJ, Schlötzer-Schrehardt U, Kruse F, Tourtas T (2017)
Publication Type: Journal article
Publication year: 2017
Journal
Book Volume: 36
Pages Range: 1576-1579
Journal Issue: 12
DOI: 10.1097/ICO.0000000000001360
Abstract
To evaluate the impact of the air bubble on endothelial cell loss using the "bubble-in-the-roll" technique during Descemet membrane endothelial keratoplasty (DMEK).Twenty DMEK grafts not suitable for transplantation were manually prepared from organ-cultured corneoscleral discs and injected into culture media using the Endoject DMEK injector (Medicel AG, Wolfhalden, Switzerland). Based on the injection method, the grafts were divided into 2 groups: In group A (n = 10), a small air bubble was placed inside the graft roll while it was in the injector. In group B (n = 10), the grafts were injected without an air bubble inside the graft roll. Main outcome measures included endothelial cell density (ECD) after graft stripping and graft injection.There were no statistically significant differences between groups A and B in donor age, storage duration, and donor ECD. ECD decreased from 1929 ± 145 cells/mm to 1796 ± 303 cells/mm after graft stripping in group A and from 1801 ± 226 cells/mm to 1709 ± 290 cells/mm in group B. ECD after graft injection further decreased to 1683 ± 291 cells/mm in group A and to 1651 ± 292 cells/mm in group B. Endothelial cell loss after graft stripping and graft injection was not statistically significant between groups A and B (P = 0.29 and P = 1, respectively).The bubble-in-the-roll technique for injection and unfolding of the graft is a safe method for graft delivery into the anterior chamber guaranteeing orientation of the graft without harming the endothelium.
Authors with CRIS profile
Yasemin Akbaba
Department of Ophthalmology
Ursula Schlötzer-Schrehardt
Medizinische Fakultät
Friedrich Kruse
Lehrstuhl für Augenheilkunde
Involved external institutions
United Kingdom (GB)How to cite
APA:
Akbaba, Y., Weller, J.M., Rössler, K., Armitage, W.J., Schlötzer-Schrehardt, U., Kruse, F., & Tourtas, T. (2017). "Bubble-in-the-Roll" Technique Using the Endoject DMEK Injector: Influence of the Air Bubble on Endothelial Cell Loss. Cornea, 36(12), 1576-1579. https://doi.org/10.1097/ICO.0000000000001360
MLA:
Akbaba, Yasemin, et al. ""Bubble-in-the-Roll" Technique Using the Endoject DMEK Injector: Influence of the Air Bubble on Endothelial Cell Loss." Cornea 36.12 (2017): 1576-1579.
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