UVA irradiation of riboflavin generates oxygen-dependent hydroxyl radicals
Sel S, Nass N, Poetzsch S, Trau S, Simm A, Kalinski T, Duncker GIW, Kruse F, Auffarth GU, Broemme HJ (2014)
Publication Type: Journal article
Publication year: 2014
Journal
Publisher: Maney Publishing
Book Volume: 19
Pages Range: 72-9
Journal Issue: 2
DOI: 10.1179/1351000213Y.0000000076
Abstract
The aim of this study was to verify the formation of hydroxyl radicals (·OH) after ultraviolet A (UVA) irradiation of riboflavin (RF) by spin trapping with 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), and electron spin resonance spectroscopy.We found that ·OH were generated via hydrogen peroxide (H?O?) formation during UVA irradiation of RF. The ·OH radicals were trapped with DMPO yielding 2-hydroxy-5,5-dimethyl-1-pyrroline-N-oxide (·DMPO-OH). The formed radical adduct (·DMPO-OH) accumulated in the RF solution. Argon equilibration of the RF solution completely blocked the formation of the ·DMPO-OH adduct whereas subsequent aeration restored radical adduct generation. The presence of catalase inhibited ·DMPO-OH generation whereas BSA had no influence on ·DMPO-OH formation. Stopping UVA irradiation led to decay of radical adducts. UVA irradiation of H?O? in the presence of DMPO but without RF also induced the formation of ·DMPO-OH adduct. When adding DMPO to an already irradiated RF solution significantly less ·DMPO-OH was formed during further irradiation. Ultraviolet-visible spectroscopy and high-performance liquid chromatography analysis of RF indicated that RF decayed during UVA irradiation.The formation of ·OH during UVA irradiation of RF may be part of the oxygen-dependent mechanism involved in the cross-linking therapy of collagen in corneal stroma.
Authors with CRIS profile
Involved external institutions
Ruprecht-Karls-Universität Heidelberg
Germany (DE)
Martin-Luther-Universität Halle-Wittenberg (MLU)
Germany (DE)
Otto-von-Guericke-Universität Magdeburg
Germany (DE)
Germany (DE)
Martin-Luther-Universität Halle-Wittenberg (MLU)
Germany (DE)
Otto-von-Guericke-Universität Magdeburg
Germany (DE)
How to cite
APA:
Sel, S., Nass, N., Poetzsch, S., Trau, S., Simm, A., Kalinski, T.,... Broemme, H.-J. (2014). UVA irradiation of riboflavin generates oxygen-dependent hydroxyl radicals. Redox Report, 19(2), 72-9. https://dx.doi.org/10.1179/1351000213Y.0000000076
MLA:
Sel, Saadettin, et al. "UVA irradiation of riboflavin generates oxygen-dependent hydroxyl radicals." Redox Report 19.2 (2014): 72-9.
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