% Encoding: UTF-8
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@inproceedings{faucris.242414168,
address = {HOBOKEN},
author = {Schmidt, Sarah and Schmid, Rafael and Arkudas, Andreas and Kengelbach-Weigand, Annika and Boßerhoff, Anja Katrin},
booktitle = {JOURNAL DER DEUTSCHEN DERMATOLOGISCHEN GESELLSCHAFT},
faupublication = {yes},
note = {CRIS-Team WoS Importer:2020-09-11},
pages = {28-29},
peerreviewed = {unknown},
publisher = {WILEY},
title = {{3D} {Bioprinting} of {Melanoma} {Cells} with commercially available {Bio} {Inks}},
year = {2020}
}
@article{faucris.261063324,
abstract = {Background: Obesity is frequently present in patients suffering from end-stage renal disease (ESRD). However, overweight kidney transplant candidates are a challenge for the transplant surgeon. Obese patients tend to develop a large abdominal panniculus after weight loss creating an area predisposed to wound-healing disorders. Due to concerns about graft survival and postoperative complications after kidney transplantation, obese patients are often refused in this selective patient cohort. The study aimed to analyze the effect of panniculectomies on postoperative complications and transplant candidacy in an interdisciplinary setting. Methods: A retrospective database review of 10 cases of abdominal panniculectomies performed in patients with ESRD prior to kidney transplantation was conducted. Results: The median body mass index was 35.2 kg/m(2) (range 28.5-53.0 kg/m(2)) at first transplant-assessment versus 31.0 kg/m(2) (range 28.0-34.4 kg/m(2)) at panniculectomy, and 31.6 kg/m(2) (range 30.3-32.4 kg/m(2)) at kidney transplantation. We observed no major postoperative complications following panniculectomy and minor wound-healing complications in 2 patients. All aside from 1 patient became active transplant candidates 6 weeks after panniculectomy. No posttransplant wound complications occurred in the transplanted patients. Conclusion: Abdominal panniculectomy is feasible in patients suffering ESRD with no major postoperative complications, thus converting previously ineligible patients into kidney transplant candidates. An interdisciplinary approach is advisable in this selective patient cohort.},
author = {Promny, Dominik and Hauck, Theresa and Cai, Aijia and Arkudas, Andreas and Heller, Katharina M. and Wullich, Bernd and Apel, Hendrik and Horch, Raymund E. and Ludolph, Ingo},
doi = {10.1159/000516678},
faupublication = {yes},
journal = {Urologia Internationalis},
note = {CRIS-Team WoS Importer:2021-07-02},
peerreviewed = {Yes},
title = {{Abdominal} {Panniculectomy} {Can} {Simplify} {Kidney} {Transplantation} in {Obese} {Patients}},
year = {2021}
}
@article{faucris.109888504,
abstract = {During the last decades, a range of excellent and promising strategies in Bone Tissue Engineering have been developed. However, the remaining major problem is the lack of vascularization. In this study, extrinsic and intrinsic vascularization strategies were combined for acceleration of vascularization. For optimal biomechanical stability of the defect site and simplifying future transition into clinical application, a primary stable and approved nanostructured bone substitute in clinically relevant size was used. An arteriovenous (AV) loop was microsurgically created in sheep and implanted, together with the bone substitute, in either perforated titanium chambers (intrinsic/extrinsic) for different time intervals of up to 18 weeks or isolated Teflon(®) chambers (intrinsic) for 18 weeks. Over time, magnetic resonance imaging and micro-computed tomography (CT) analyses illustrate the dense vascularization arising from the AV loop. The bone substitute was completely interspersed with newly formed tissue after 12 weeks of intrinsic/extrinsic vascularization and after 18 weeks of intrinsic/extrinsic and intrinsic vascularization. Successful matrix change from an inorganic to an organic scaffold could be demonstrated in vascularized areas with scanning electron microscopy and energy dispersive X-ray spectroscopy. Using the intrinsic vascularization method only, the degradation of the scaffold and osteoclastic activity was significantly lower after 18 weeks, compared with 12 and 18 weeks in the combined intrinsic-extrinsic model. Immunohistochemical staining revealed an increase in bone tissue formation over time, without a difference between intrinsic/extrinsic and intrinsic vascularization after 18 weeks. This study presents the combination of extrinsic and intrinsic vascularization strategies for the generation of an axially vascularized bone substitute in clinically relevant size using a large animal model. The additional extrinsic vascularization promotes tissue ingrowth and remodeling processes of the bone substitute. Extrinsic vessels contribute to faster vascularization and finally anastomose with intrinsic vasculature, allowing microvascular transplantation of the bone substitute after a shorter prevascularization time than using the intrinsic method only. It can be reasonably assumed that the usage of perforated chambers can significantly reduce the time until transplantation of bone constructs. Finally, this study paves the way for further preclinical testing for proof of the concept as a basis for early clinical applicability.},
author = {Weigand, Annika and Beier, Justus and Heß, Andreas and Gerber, Thomas and Arkudas, Andreas and Horch, Raymund E. and Boos, Anja},
doi = {10.1089/ten.TEA.2014.0568},
faupublication = {yes},
journal = {Tissue Engineering: Parts A, B, and C},
note = {EVALuna2:15582},
pages = {1680-94},
peerreviewed = {unknown},
title = {{Acceleration} of vascularized bone tissue-engineered constructs in a large animal model combining intrinsic and extrinsic vascularization},
volume = {21},
year = {2015}
}
@article{faucris.119410104,
abstract = {Signal transducer and activator of transcription 3 (STAT3) is phosphorylated by various kinases, several of which have been implicated in aberrant fibroblast activation in fibrotic diseases including systemic sclerosis (SSc). Here we show that profibrotic signals converge on STAT3 and that STAT3 may be an important molecular checkpoint for tissue fibrosis. STAT3 signaling is hyperactivated in SSc in a TGF?-dependent manner. Expression profiling and functional studies in vitro and in vivo demonstrate that STAT3 activation is mediated by the combined action of JAK, SRC, c-ABL, and JNK kinases. STAT3-deficient fibroblasts are less sensitive to the pro-fibrotic effects of TGF?. Fibroblast-specific knockout of STAT3, or its pharmacological inhibition, ameliorate skin fibrosis in experimental mouse models. STAT3 thus integrates several profibrotic signals and might be a core mediator of fibrosis. Considering that several STAT3 inhibitors are currently tested in clinical trials, STAT3 might be a candidate for molecular targeted therapies of SS},
author = {Chakraborty, Debomita and Sumova, Barbora and Mallano, Tatjana and Chen, Chih-Wei and Distler, Alfiya and Bergmann, Christina and Ludolph, Ingo and Horch, Raymund E. and Gelse, Kolja and Ramming, Andreas and Distler, Oliver and Schett, Georg and Senolt, Ladislav and Distler, Jörg},
doi = {10.1038/s41467-017-01236-6},
faupublication = {yes},
journal = {Nature Communications},
note = {EVALuna2:13455},
pages = {1130},
peerreviewed = {Yes},
title = {{Activation} of {STAT3} integrates common profibrotic pathways to promote fibroblast activation and tissue fibrosis},
volume = {8},
year = {2017}
}
@article{faucris.252106726,
abstract = {Intravital microscopy (IVM) study approach offers several advantages over in vitro, ex vivo, and 3D models. IVM provides real-time imaging of cellular events, which provides us a comprehensive picture of dynamic processes. Rapid improvement in microscopy techniques has permitted deep tissue imaging at a higher resolution. Advances in fluorescence tagging methods enable tracking of specific cell types. Moreover, IVM can serve as an important tool to study different stages of tissue regeneration processes. Furthermore, the compatibility of different tissue engineered constructs can be analyzed. IVM is also a promising approach to investigate host reactions on implanted biomaterials. IVM can provide instant feedback for improvising tissue engineering strategies. In this review, we aim to provide an overview of the requirements and applications of different IVM approaches. First, we will discuss the history of IVM development, and then we will provide an overview of available optical modalities including the pros and cons. Later, we will summarize different fluorescence labeling methods. In the final section, we will discuss well-established chronic and acute IVM models for different organs.},
author = {Vaghela, Ravikumar and Arkudas, Andreas and Horch, Raymund E. and Hessenauer, Maximilian},
doi = {10.3389/fbioe.2021.627462},
faupublication = {yes},
journal = {Frontiers in Bioengineering and Biotechnology},
keywords = {biomaterial; fluorescence; in vivo; intravital microscopy; leukocyte recruitment; tissue engineering},
note = {CRIS-Team Scopus Importer:2021-03-19},
peerreviewed = {Yes},
title = {{Actually} {Seeing} {What} {Is} {Going} on – {Intravital} {Microscopy} in {Tissue} {Engineering}},
volume = {9},
year = {2021}
}
@article{faucris.114034184,
abstract = {Mesenchymal stem cells can be isolated from a variety of different sources, each having their own peculiar merits and drawbacks. Although a number of studies have been conducted comparing these stem cells for their osteo-differentiation ability, these are mostly done in culture plastics. We have selected stem cells from either adipose tissue (ADSCs) or bone marrow (BMSCs) and studied their differentiation ability in highly porous three-dimensional (3D) 45S5 Bioglass®-based scaffolds. Equal numbers of cells were seeded onto 5 × 5 × 4 mm(3) scaffolds and cultured in vitro, with or without osteo-induction medium. After 2 and 4 weeks, the cell-scaffold constructs were analysed for cell number, cell spreading, viability, alkaline phosphatase activity and osteogenic gene expression. The scaffolds with ADSCs displayed osteo-differentiation even without osteo-induction medium; however, with osteo-induction medium osteogenic differentiation was further increased. In contrast, the scaffolds with BMSCs showed no osteo-differentiation without osteo-induction medium; after application of osteo-induction medium, osteo-differentiation was confirmed, although lower than in scaffolds with ADSCs. In general, stem cells in 3D bioactive glass scaffolds differentiated better than cells in culture plastics with respect to their ALP content and osteogenic gene expression. In summary, 45S5 Bioglass-based scaffolds seeded with ADSCs are well-suited for possible bone tissue-engineering applications. Induction of osteogenic differentiation appears unnecessary prior to implantation in this specific setting. Copyright © 2013 John Wiley & Sons, Ltd.},
author = {Rath, Subha N. and Nooeaid, Patcharakamon and Arkudas, Andreas and Beier, Justus and Strobel, Leonie and Brandl, Andreas and Roether, Judith and Horch, Raymund E. and Boccaccini, Aldo R. and Kneser, Ulrich},
doi = {10.1002/term.1849},
faupublication = {yes},
journal = {Journal of Tissue Engineering and Regenerative Medicine},
note = {UnivIS-Import:2015-03-09:Pub.2014.tech.IW.LM.adipos},
pages = {E497-E509},
peerreviewed = {unknown},
title = {{Adipose}- and bone marrow-derived mesenchymal stem cells display different osteogenic differentiation patterns in {3D} bioactive glass-based scaffolds},
volume = {10},
year = {2013}
}
@article{faucris.233864628,
abstract = {Lipotransfer has been applied in breast augmentation surgery for several years and the resident adipose-derived stem cells (ASCs) play an important role in enhancing fat graft survival. However, the interaction between ASCs and mammary epithelium is not fully understood. Many studies have shown that ASCs have a tumor-supportive effect in breast cancer. To the best of our knowledge, this is the first study on the effect of mammary epithelial cells on the human ASCs in 3D culture. ASCs were cultivated on matrigel in the conditioned medium (CM) prepared from a human breast epithelial cell line (HBL-100). The ASCs formed KRT18-positive acini-like structures after stimulation with breast epithelial cells. The expression of epithelial genes (CDH1 and KRT18) was up-regulated while the expression of mesenchymal specific genes (CDH2 and VIM) was down-regulated as determined by qRT-PCR. The stemness marker (CD29) and angiogenic factors (CD31 and VEGF) were also down-regulated as examined by immunofluorescence. In addition, the CM obtained from HBL-100 enhanced the migration and inhibited the adipogenic differentiation of ASCs. These results demonstrate that ASCs have the ability to transform into epithelial-like cells when cultured with mammary epithelial cells. Given these observations, we infer that ASCs have a positive effect on lipotransfer, not only due to their ability to secrete growth factors, but also due to their direct participation in the formation of new breast tissue.
},
author = {Tong, Jing and Mou, Shan and Xiong, Lingyun and Wang, Zhenxing and Wang, Rongrong and Weigand, Annika and Yuan, Quan and Horch, Raymund E. and Sun, Jiaming and Yang, Jie},
doi = {10.1371/journal.pone.0204077},
faupublication = {yes},
journal = {PLoS ONE},
note = {EVALuna2:211468},
peerreviewed = {Yes},
title = {{Adipose}-derived mesenchymal stem cells formed acinar-like structure when stimulated with breast epithelial cells in three-dimensional culture},
volume = {13},
year = {2018}
}
@article{faucris.209641747,
abstract = {BACKGROUND: Breast cancer is the most common malignancy in women affecting one out of eight females throughout their lives. Autotaxin (ATX) is upregulated in breast cancer which results in increased lysophosphatidic acid (LPA) formation within the tumor. This study's aim was to identify the role of different mammary cell populations within the ATX-LPA axis.
METHODS: Epithelial-cell-adhesion-molecule-positive (EpCAM) and -negative cells from breast tumors, adipose-derived stem cells (ADSCs) of tumor-adjacent and tumor-distant mammary fat were isolated and compared to healthy ADSCs, mammary epithelial cells (HMECs), and mesenchymal cells (MES) of healthy mammary tissue (n = 4 each) and further to well-established breast (cancer) cell lines.
RESULTS: mRNA expression analyses revealed that ADSCs and MES largely expressed LPA receptor 1 (LPAR1) while epithelial cells mainly expressed LPAR6. LPA 18:1 activated all the cell populations and cell lines by rise in cytosolic free calcium concentrations. MES and ADSCs expressed ATX whereas epithelial cells did not. ADSCs revealed the highest expression in ATX with a significant decline after adipogenic differentiation in healthy ADSCs, whereas ATX expression increased in ADSCs from tumor patients. Breast (cancer) cell lines did not express ATX. Transmigration of MES was stimulated by LPA whereas an inhibitory effect was observed in epithelial cells with no differences between tumors and healthy cells. Triple-negative breast cancer (TNBC) cell lines were also stimulated and the transmigration partly inhibited using the LPA receptor antagonist Ki16425.
CONCLUSIONS: We here show that each mammary cell population plays a different role in the ATX-LPA axis with ADSCs and adipocytes being the main source of ATX in tumor patients in our experimental setting. Inhibitors of this axis may therefore present a valuable target for pharmacological therapies.},
author = {Schmid, Rafael and Wolf, Katharina and Robering, Jan Willem and Strauss, Selina and Strissel, Pamela and Strick, Reiner and Rübner, Matthias and Fasching, Peter and Horch, Raymund E. and Kremer, Andreas and Boos, Anja and Weigand, Annika},
doi = {10.1186/s12885-018-5166-z},
faupublication = {yes},
journal = {BMC Cancer},
note = {EVALuna2:35125},
peerreviewed = {Yes},
title = {{ADSCs} and adipocytes are the main producers in the autotaxin-lysophosphatidic acid axis of breast cancer and healthy mammary tissue in vitro},
volume = {18},
year = {2018}
}
@article{faucris.121875424,
abstract = {This preliminary ex vivo study aimed to clarify the pathophysiologic mechanisms of fat tissue depletion by subcutaneous drug application. Therefore, the lipolytic effects of phosphatidylcholine plus deoxycholate (Lipostabil) (L) and of deoxycholate (DC) alone were compared with those of sodium chloride (NaCl) and hydrogen peroxide (H2O2) as control agents. The study enrolled 10 patients receiving abdominoplasty. The treatment periods for each sample and solution were 1, 3, 5, and 7 h. The samples were analyzed morphologically using hematoxylin-eosin (H&E) staining and also immunohistochemically using Caspase 3 and tumor necrosis factor (TNF)-alpha. Morphologic changes were seen best after 5 h of application time. Except for NaCl, all the samples in the H&E staining showed marked damage of adipocyte cell membranes, with the greatest disruption of normal cell architecture after hydrogen peroxide (H2O2) application. Immunohistochemistry using TNF-alpha showed positive results for the deoxycholate and Lipostabil samples and highly positive results for the H2O2 sample. Data from this study indicate that Lipostabil and deoxycholate induce pathways of cell necrosis involving TNF-alpha. These short-term experiments indicate that Lipostabil affects fat tissue in the way of a chemical-toxic destruction rather than via a physiologically induced, programmed cell death.},
author = {Huebner, Nina-Fee and Horch, Raymund E. and Polykandriotis, Elias and Rau, Tilman and Dragu, Adrian},
doi = {10.1007/s00266-014-0371-x},
faupublication = {yes},
journal = {Aesthetic Plastic Surgery},
note = {EVALuna2:6587},
pages = {976-84},
peerreviewed = {Yes},
title = {{A} histopathologic and immunohistochemical study on liquification of human adipose tissue ex vivo},
volume = {38},
year = {2014}
}
@article{faucris.119746924,
abstract = {We analysed the outcomes of a series of 100 consecutive patients with anorectal cancer with neoadjuvant radiochemotherapy and abdominoperineal exstirpation or total pelvic exenteration, who received a transpelvic vertical rectus abdominis myocutaneous (VRAM) flap for pelvic, vaginal and/or perineal reconstruction and compare a cohort to patients without VRAM flaps.Within a 10-year period (2003-2013) in our institution 924 patients with rectal cancer stage y0 to y IV were surgically treated. Data of those 100 consecutive patients who received a transpelvic VRAM flap were collected and compared to patients without flaps.In 100 consecutive patients with transpelvic VRAM flaps, major donor site complications occurred in 6 %, VRAM-specific perineal wound complications were observed in 11 % of the patients and overall 30-day mortality was 2 %.The VRAM flap is a reliable and safe method for pelvic reconstruction in patients with advanced disease requiring pelvic exenteration and irradiation, with a relatively low rate of donor and recipient site complications. In this first study, to compare a large number of patients with VRAM flap reconstruction to patients without pelvic VRAM flap reconstruction, a clear advantage of simultaneous pelvic reconstruction is demonstrated.},
author = {Horch, Raymund E. and Hohenberger, Werner and Eweida, A. and Kneser, U. and Weber, K. and Arkudas, Andreas and Merkel, Susanne and Göhl, Jonas and Beier, Justus},
doi = {10.1007/s00384-014-1868-0},
faupublication = {yes},
journal = {International Journal of Colorectal Disease},
note = {EVALuna2:15167},
pages = {813-23},
peerreviewed = {Yes},
title = {{A} hundred patients with vertical rectus abdominis myocutaneous ({VRAM}) flap for pelvic reconstruction after total pelvic exenteration},
volume = {29},
year = {2014}
}
@article{faucris.281712595,
abstract = {The treatment of large-scale skin wounds remains a therapeutic challenge. In most cases there is not enough autologous material available for full coverage. Cultured epithelial autografts are efficient in restoring the lost epidermal cover; however, they have some disadvantages, such as difficult application and protracted cell cultivation periods. Transplanting a sprayed keratinocyte suspension in fibrin sealant as biological carrier is an option to overcome those disadvantages. Here, we studied different seeding techniques regarding their applicability and advantages on cell survival, attachment, and outgrowth in vitro and thereby improve the cell transfer to the wound bed. Human primary keratinocytes were suspended in a fibrin sealant. WST-8 assay was used to evaluate the vitality for 7 days. Furthermore, the cells were labeled with CellTracker (TM) CM-Di-I and stained with a life/dead staining. Cell morphology, shape, and distribution were microscopically analyzed. There was a significant increase in vitality while cultivating the cells in fibrin. Sprayed cells were considerably more homogenously distributed. Sprayed cells reached the confluent state earlier than dripped cells. There was no difference in the vitality and morphology in both groups over the observation period. These findings indicate that the sprayed keratinocytes are superior to the application of the cells as droplets. The sprayed application may offer a promising therapeutic option in the treatment of large chronic wounds.},
author = {Sörgel, Celena and Schmid, Rafael and Kengelbach-Weigand, Annika and Promny, Theresa and Horch, Raymund E.},
doi = {10.3390/jcm11175032},
faupublication = {yes},
journal = {Journal of Clinical Medicine},
note = {CRIS-Team WoS Importer:2022-09-16},
peerreviewed = {Yes},
title = {{Air}-{Pressure}-{Supported} {Application} of {Cultured} {Human} {Keratinocytes} in a {Fibrin} {Sealant} {Suspension} as a {Potential} {Clinical} {Tool} for {Large}-{Scale} {Wounds}},
volume = {11},
year = {2022}
}
@article{faucris.266783067,
abstract = {This study presents a novel surgical technique for the reconstruction of highly challenging large lower back defects. In this case, a 72-year-old man initially diagnosed with renal cell carcinoma received nephrectomy followed by the dissection of an iliac crest metastasis and repeated high-dose irradiation therapy. Several years later, an osteocutaneous fistula at the right caudal posterior trunk made the reconstruction of the lower back defect necessary. High-dose irradiation of the lower back and poor vascular conditions at the pelvic region disqualified the patient for previously published local or free flap options. The initial strategy of an arteriovenous loop anastomosed to the femoral vessels and a free latissimus dorsi flap transfer had to be withdrawn due to repeated intraoperative loop thrombosis. For that reason, the entire latissimus dorsi muscle was used as a myocutaneous propeller flap receiving its blood supply solely through a single dorsal intercostal artery perforator. The flap survived completely and no fistulous formation occurred postoperatively. The time to complete wound healing was 4 months. This new technique is considered a valuable addition for the reconstruction of challenging posterior caudal trunk defects.},
author = {Geierlehner, Alexander and Ludolph, Ingo and Arkudas, Andreas and Horch, Raymund E.},
doi = {10.1097/GOX.0000000000003881},
faupublication = {yes},
journal = {Plastic and Reconstructive Surgery Global Open},
note = {CRIS-Team WoS Importer:2021-12-03},
peerreviewed = {Yes},
title = {{A} {Myocutaneous} {Latissimus} {Dorsi} {Propeller} {Flap} {Based} on a {Single} {Dorsal} {Intercostal} {Perforator}},
volume = {9},
year = {2021}
}
@inproceedings{faucris.113902404,
author = {Schubert, Dirk W. and Horch, Raymund E. and Kaschta, Joachim},
booktitle = {13th Bayreuth Polymer Symposium},
date = {2013-09-15/2013-09-17},
faupublication = {yes},
peerreviewed = {unknown},
title = {{Analysis} of gel from silicone breast implants made by {Poly} {Implant} {Prothese} and other manufactures},
venue = {Bayreuth},
year = {2013}
}
@inproceedings{faucris.111665884,
author = {Daenicke, Jonas and Schubert, Dirk W. and Gedde, Ulf and Hedenqvist, Mikael and Linde, Erik and Sigl, Thomas and Horch, Raymund E.},
booktitle = {Book of Abstracts},
date = {2016-05-09/2016-05-13},
faupublication = {yes},
pages = {211-212},
peerreviewed = {unknown},
title = {{Analysis} {Of} {The} {Diffusion} {Properties} {Of} {Cyclic} {Siloxanes} {Through} {Silicone} {Elastomers} - {Influence} {Of} {Crosslinking} {And} {Penetrant} {Size}},
venue = {Posen},
year = {2016}
}
@article{faucris.271000968,
abstract = {Background: The complex interplay of single wrist bones acting in combination with their ligamentous connections is still not fully understood. In this regard various theories exist, divisible in columnar and ring/row theories. The object of this study was to examine the mobility of the individual carpal bones as well as the ulna and metacarpals relative to each other in wrists of cadaveric hands using CT scans. Methods: The regular wrist mobility of a total of 21 cadaveric hands was examined by CT imaging in neutral position, radial/ulnar abduction as well as wrist flexion and extension. The data were evaluated as 3D models by using a standardized global coordinate system and object coordinate systems. Rotation and translation of each carpal bone as well as radius/ulna and all metacarpal bones were evaluated. Results: The principal motion took place in the carpus between the radius and the proximal carpal row followed by the midcarpal joint and the carpometacarpal joints and not mainly between the individual bones of a row. The scaphoid moves out of its row aggregate mainly during flexion and adapts to the motion of the distal carpal row. The trapezium and first metacarpal bones play a specific role detached from the remaining bones. Conclusions: With this study, a better understanding of the motion of the individual bones of the carpus, the metacarpals and the radius/ulna is shown. The study supports the row theory, where most motion takes place between the individual rows and not between the carpal bones, leaving the scaphoid and the first ray in a special role between the rows.},
author = {Gill, Dominik and Lyer, Stefan and Alexiou, Christoph and Fried, Frederik and Buder, Thomas and Neuhuber, Winfried and Jacxsens, Matthijs and Horch, Raymund E. and Arkudas, Andreas},
doi = {10.1016/j.aanat.2022.151909},
faupublication = {yes},
journal = {Annals of Anatomy-Anatomischer Anzeiger},
keywords = {3-dimensional Computed Tomography; Biomechanical Theory; Wrist},
note = {CRIS-Team Scopus Importer:2022-03-18},
peerreviewed = {Yes},
title = {{Anatomical} study of all carpal and adjoining bones of the wrist using {3D} {CT} reconstruction –{Finding} the ultimate biomechanical theory},
volume = {242},
year = {2022}
}
@article{faucris.313203622,
abstract = {Background: Compression of the ulnar nerve at the elbow within the cubital tunnel is related to the anatomical structures and is generally believed to be caused by Osborne's ligament (also known as the cubital retinaculum). However, in rare cases an anatomical variation of the developmental peculiarity of a remaining anconeus epitrochlearis muscle may be responsible for the disease. Methods: We present a series of five cases in which an anconeus epitrochlearis muscle was found as the cause of illness. Results: All patients presented with typical symptoms of numbness and tingling in the hand and ulnar fingers, and recurring pain as well as weakness of the ulnar innervated muscles. With neurophysiologically confirmed diminished nerve conduction velocity and unsuccessful conservative treatment, surgical decompression revealed an anconeus epitrochlearis muscle as the reason of compression. Full symptom relief was achieved immediately after the procedure in all cases. Conclusions: This article strives to call attention to this entity when diagnosing ulnar nerve compression. Myectomy and medial epicondylectomy is the preferred treatment option in such cases.},
author = {Grüner, Jasmin and Paulsen, Friedrich and Barth, Anna and Horch, Raymund E.},
doi = {10.1016/j.aanat.2023.152152},
faupublication = {yes},
journal = {Annals of Anatomy-Anatomischer Anzeiger},
keywords = {Cubital tunnel syndrome; Musculus anconeus epitrochlearis; Musculus epitrochleoanconeus; Sulcus ulnaris},
note = {CRIS-Team Scopus Importer:2023-10-27},
peerreviewed = {Yes},
title = {{Anconeus} epitrochlearis muscle (epitrochlearisanconeus muscle; {Musculus} epitrochleoanconeus) with cubital tunnel syndrome – a rare but relevant clinical entity},
volume = {250},
year = {2023}
}
@article{faucris.270582348,
abstract = {Two-dimensional (2D) cancer models have been the standard for drug
development over the past few years, but they frequently do not resemble
in vivo properties adequately. 3D models are superior in many aspects
and are, therefore, more similar to human pathophysiology. Over the past
years, the emerging field of biofabrication has made significant
advances, resulting in even more sophisticated 3D models. With this
study, a hydrogel is created for biofabrication that is suitable for
mimicking the tumor microenvironment in vitro and is further tested as a
new vascularized melanoma model in vivo. The alginate/hyaluronic
acid/gelatin bioink shows good shape-fidelity, high cell survival rates,
and enables successful cultivation of melanoma cells and
adipose-derived stem cells as well as cell differentiation in vitro. In
vivo, in the arteriovenous loop model, it proves to be a unique method
to study melanoma progression, tumor vascularization, and ultimately and
reliably metastases in an isolated and controlled environment. These
results show that this 3D model is very application-oriented for
molecular research and therapy developmen},
author = {Schmid, Rafael and Schmidt, Sonja and Detsch, Rainer and Horder, Hannes and Blunk, Torsten and Schrüfer, Stefan and Schubert, Dirk W. and Fischer, Lena and Thievessen, Ingo and Heltmann-Meyer, Stefanie and Steiner, Dominik and Schneidereit, Dominik and Friedrich, Oliver and Grüneboom, Anika and Amouei, Hanna and Wajant, Harald and Horch, Raymund E. and Boßerhoff, Anja Katrin and Arkudas, Andreas and Kengelbach-Weigand, Annika},
doi = {10.1002/adfm.202107993},
faupublication = {yes},
journal = {Advanced Functional Materials},
peerreviewed = {Yes},
title = {{A} new printable alginate / hyaluronic acid / gelatin hydrogel suitable for biofabrication of in vitro and in vivo metastatic melanoma models},
url = {https://onlinelibrary.wiley.com/doi/full/10.1002/adfm.202107993},
year = {2021}
}
@article{faucris.265349206,
abstract = {Two-dimensional (2D) cancer models have been the standard for drug development over the past few years, but they frequently do not resemble in vivo properties adequately. 3D models are superior in many aspects and are, therefore, more similar to human pathophysiology. Over the past years, the emerging field of biofabrication has made significant advances, resulting in even more sophisticated 3D models. With this study, a hydrogel is created for biofabrication that is suitable for mimicking the tumor microenvironment in vitro and is further tested as a new vascularized melanoma model in vivo. The alginate/hyaluronic acid/gelatin bioink shows good shape-fidelity, high cell survival rates, and enables successful cultivation of melanoma cells and adipose-derived stem cells as well as cell differentiation in vitro. In vivo, in the arteriovenous loop model, it proves to be a unique method to study melanoma progression, tumor vascularization, and ultimately and reliably metastases in an isolated and controlled environment. These results show that this 3D model is very application-oriented for molecular research and therapy development.},
author = {Schmid, Rafael and Schmidt, Sonja and Detsch, Rainer and Horder, Hannes and Blunk, Torsten and Schrüfer, Stefan and Schubert, Dirk W. and Fischer, Lena and Thievessen, Ingo and Heltmann-Meyer, Stefanie and Steiner, Dominik and Schneidereit, Dominik and Friedrich, Oliver and Grüneboom, Anika and Amouei, Hanna and Wajant, Harald and Horch, Raymund E. and Boßerhoff, Anja Katrin and Arkudas, Andreas and Kengelbach-Weigand, Annika},
doi = {10.1002/adfm.202107993},
faupublication = {yes},
journal = {Advanced Functional Materials},
keywords = {bioinks; metastasis; stem cells; tissue engineering; tumors},
note = {CRIS-Team Scopus Importer:2021-10-22},
peerreviewed = {Yes},
title = {{A} {New} {Printable} {Alginate}/{Hyaluronic} {Acid}/{Gelatin} {Hydrogel} {Suitable} for {Biofabrication} of {In} {Vitro} and {In} {Vivo} {Metastatic} {Melanoma} {Models}},
year = {2021}
}
@article{faucris.270640008,
author = {Schmid, Rafael and Schmidt, Sonja and Detsch, Rainer and Horder, Hannes and Blunk, Torsten and Schrüfer, Stefan and Schubert, Dirk W. and Fischer, Lena and Thievessen, Ingo and Heltmann-Meyer, Stefanie and Steiner, Dominik and Schneidereit, Dominik and Friedrich, Oliver and Grüneboom, Anika and Amouei, Hanna and Wajant, Harald and Horch, Raymund E. and Boßerhoff, Anja Katrin and Arkudas, Andreas and Kengelbach-Weigand, Annika},
doi = {10.1002/adfm.202107993},
faupublication = {yes},
journal = {Advanced Functional Materials},
month = {Jan},
peerreviewed = {Yes},
title = {{A} new printable alginate / hyaluronic acid / gelatin hydrogel suitable for biofabrication of in vitro and in vivo metastatic melanoma models},
volume = {Volume 32},
year = {2022}
}
@article{faucris.277808529,
abstract = {Animal models are important tools to investigate the pathogenesis and develop treatment strategies for breast cancer in humans. In this study, we developed a new three-dimensional in vivo arteriovenous loop model of human breast cancer with the aid of biodegradable materials, including fibrin, alginate, and polycaprolactone. We examined the in vivo effects of various matrices on the growth of breast cancer cells by imaging and immunohistochemistry evaluation. Our findings clearly demonstrate that vascularized breast cancer microtissues could be engineered and recapitulate the in vivo situation and tumor-stromal interaction within an isolated environment in an in vivo organism. Alginate–fibrin hybrid matrices were considered as a highly powerful material for breast tumor engineering based on its stability and biocompatibility. We propose that the novel tumor model may not only serve as an invaluable platform for analyzing and understanding the molecular mechanisms and pattern of oncologic diseases, but also be tailored for individual therapy via transplantation of breast cancer patient-derived tumors.},
author = {An, Ran and Strissel, Pamela and Al-Abboodi, Majida and Robering, Jan Willem and Reakasame, Supachai and Eckstein, Markus and Peddi, Ajay and Hauck, Theresa and Bäuerle, Tobias and Boccaccini, Aldo R. and Youssef, Almoatazbellah and Sun, Jiaming and Strick, Reiner and Horch, Raymund E. and Boos, Anja and Kengelbach-Weigand, Annika},
doi = {10.3390/bioengineering9070280},
faupublication = {yes},
journal = {Bioengineering},
keywords = {animal model; arteriovenous loop; breast cancer},
note = {CRIS-Team Scopus Importer:2022-07-15},
peerreviewed = {Yes},
title = {{An} {Innovative} {Arteriovenous} ({AV}) {Loop} {Breast} {Cancer} {Model} {Tailored} for {Cancer} {Research}},
volume = {9},
year = {2022}
}
@article{faucris.280973959,
abstract = {The application of lipotransfer after breast-conserving therapy (BCT) and irradiation in breast cancer patients is an already widespread procedure for reconstructing volume deficits of the diseased breast. Nevertheless, the safety of lipotransfer has still not been clarified yet due to contradictory data. The goal of this in vitro study was to further elucidate the potential effects of lipotransfer on the irradiated remaining breast tissue. The mammary epithelial cell line MCF-10A was co-cultured with the fibroblast cell line MRC-5 and irradiated with 2 and 5 Gy. Afterwards, cells were treated with conditioned medium (CM) from adipose-derived stem cells (ADSC), and the effects on the cellular functions of MCF-10A cells and on gene expression at the mRNA level in MCF-10A and MRC-5 cells were analyzed. Treatment with ADSC CM stimulated transmigration and invasion and decreased the surviving fraction of MCF-10A cells. Further, the expression of cytokines, extracellular, and mesenchymal markers was enhanced in mamary epithelial cells. Only an effect of ADSC CM on irradiated fibroblasts could be observed. The present data suggest epithelial–mesenchymal transition-like changes in the epithelial mammary breast cell line. Thus, the benefits of lipotransfer after BCT should be critically weighed against its possible risks for the affected patients.
◦ to 53◦. A number of complications, in all steps of the treatment, were noted in a total of 36.4% of patients, including the development of fractures (16.4%), followed by vessel injury, pin infections, and complex regional pain syndrome (5%). Conclusions: Additional skeletal distraction improves the range of motion of severely contracted joints in Dupuytren’s disease. Nevertheless, careful patient selection is necessary, due to the moderate rate of complications.},
author = {Müller-Seubert, Wibke and Cai, Aijia and Arkudas, Andreas and Ludolph, Ingo and Fritz, Niklas and Horch, Raymund E.},
doi = {10.3390/jpm12030378},
faupublication = {yes},
journal = {Journal of Personalized Medicine},
keywords = {Distracted driving; Dupuytren contracture; Joint dislocation},
note = {CRIS-Team Scopus Importer:2022-03-25},
peerreviewed = {Yes},
title = {{A} {Personalized} {Approach} to {Treat} {Advanced} {Stage} {Severely} {Contracted} {Joints} in {Dupuytren}’s {Disease} with a {Unique} {Skeletal} {Distraction} {Device}—{Utilizing} {Modern} {Imaging} {Tools} to {Enhance} {Safety} for the {Patient}},
volume = {12},
year = {2022}
}
@article{faucris.121177144,
abstract = {Being able to segment the esophagus without user interaction from 3-D CT data is of high value to radiologists during oncological examinations of the mediastinum. The segmentation can serve as a guideline and prevent confusion with pathological tissue. However, limited contrast to surrounding structures and versatile shape and appearance make segmentation a challenging problem. This paper presents a multistep method. First, a detector that is trained to learn a discriminative model of the appearance is combined with an explicit model of the distribution of respiratory and esophageal air. In the next step, prior shape knowledge is incorporated using a Markov chain model. We follow a detect and connect approach to obtain the maximum a posteriori estimate of the approximate esophagus shape from hypothesis about the esophagus contour in axial image slices. Finally, the surface of this approximation is nonrigidly deformed to better fit the boundary of the organ. The method is compared to an alternative approach that uses a particle filter instead of a Markov chain to infer the approximate esophagus shape, to the performance of a human observer and also to state of the art methods, which are all semiautomatic. Cross-validation on 144 CT scans showed that the Markov chain based approach clearly outperforms the particle filter. It segments the esophagus with a mean error of 1.80 mm in less than 16 s on a standard PC. This is only 1 mm above the interobserver variability and can compete with the results of previously published semiautomatic methods. © 2011 IEEE.},
author = {Feulner, Johannes and Zhou, S. Kevin and Hammon, Matthias and Seifert, Sascha and Huber, Martin and Comaniciu, Dorin and Hornegger, Joachim and Cavallaro, Alexander Josef},
doi = {10.1109/TMI.2011.2112372},
faupublication = {yes},
journal = {IEEE Transactions on Medical Imaging},
pages = {1252-1264},
peerreviewed = {Yes},
title = {{A} probabilistic model for automatic segmentation of the esophagus in 3-{D} {CT} scans},
volume = {30},
year = {2011}
}
@article{faucris.230285274,
abstract = {Bacterial
contamination is hypothesized to be one reason for the development of
capsular contracture after alloplastic breast reconstruction using
silicone breast implants. The role of fungal colonization or infection
in this context as well as the question if microorganisms can penetrate
the shell of silicone breast implants remains an unresolved question to
date. Therefore, the aim of this study was to assess whether fungal
spores are able to penetrate the shell of silicone implants.
In an experimental in vitro setup with different arrangements of growth compartments, silicone chambers were placed in culture dishes filled with Aspergillus minimal medium or liquid culture medium. Inoculation was performed with conidia of Aspergillus fumigatus and incubated for seven days. On a daily basis, plates were inspected for conidial germination and hyphal growth.
In none of the different experimental settings nutrients or hyphae of Aspergillus fumigatus were able to penetrate the silicone material.ConclusionsFungal
spores and hyphae do not permeate through an intact silicone shell used
in breast implants; thus, the silicone material serves as an
impenetrable barrie},
author = {Horch, Raymund E. and Krappmann, Sven and Duemig, Michaela and Schmitz, Marweh and Kengelbach-Weigand, Annika and Schubert, Dirk W. and Kremser, Thomas and Bogdan, Christian and Ludolph, Ingo},
doi = {10.1097/SAP.0000000000002135},
faupublication = {yes},
journal = {Annals of Plastic Surgery},
keywords = {Aspergillus fumigatus;penetration;silicone implants;breast},
pages = {306-309},
peerreviewed = {Yes},
title = {{Aspergillus} fumigatus {Spores} {Are} {Not} {Able} to {Penetrate} {Silicone} {Breast} {Implant} {Shells}},
url = {https://www.ncbi.nlm.nih.gov/pubmed/31800548},
volume = {85},
year = {2019}
}
@article{faucris.107924344,
abstract = {Preventing ischemia-related cell damage is a priority when preserving tissue for transplantation. Perfusion protocols have been established for a variety of applications and proven to be superior to procedures used in clinical routine. Extracorporeal perfusion of muscle tissue though cumbersome is highly desirable since it is highly susceptible to ischemia-related damage. To show the efficacy of different perfusion protocols external field stimulation can be used to immediately visualize improvement or deterioration of the tissue during active and running perfusion protocols. This method has been used to show the superiority of extracorporeal perfusion using porcine rectus abdominis muscles perfused with heparinized saline solution. Perfused muscles showed statistically significant higher ability to exert force compared to nonperfused ones. These findings can be confirmed using Annexin V as marker for cell damage, perfusion of muscle tissue limits damage significantly compared to nonperfused tissue. The combination of extracorporeal perfusion and external field stimulation may improve organ conservation research.},
author = {Taeger, Christian and Friedrich, Oliver and Dragu, Adrian and Weigand, Annika and Hobe, Frieder and Drechsler, Caroline and Geppert, Carol-Immanuel and Arkudas, Andreas and Münch, Frank and Buchholz, Rainer and Pollmann, Charlotte and Schramm, Axel and Birkholz, Torsten and Horch, Raymund E. and Präbst, Konstantin and Buchholz, Rainer},
doi = {10.1038/srep11956},
faupublication = {yes},
journal = {Scientific Reports},
pages = {11956},
peerreviewed = {Yes},
title = {{Assessing} viability of extracorporeal preserved muscle transplants using external field stimulation: a novel tool to improve methods prolonging bridge-to-transplantation time.},
volume = {5},
year = {2015}
}
@article{faucris.219406593,
abstract = {Post-mastectomy autologous reconstruction with abdominal tissue has evolved over the past 4 decades and is a common reconstructive modality today. To gain more insight into this evolution, we performed an analysis of the 100 most commonly cited articles focusing on autologous breast reconstruction with transverse rectus abdominis musculocutaneous (TRAM) or deep inferior epigastric perforator (DIEP) flaps. A review of the ISI Web of Knowledge database was performed. Only peer-reviewed articles in English were included for analysis. Articles were ranked by their total citations as well as citation density (citations divided by years since publication). The 100 most cited articles were analyzed by their bibliographic parameters. The 100 most cited articles were published in 12 journals. The highest ranked plastic surgery journal published almost 2/3 of the articles. All articles were published within 23 years and marked the "rising age" of autologous breast reconstruction with TRAM and DIEP flaps. The focus of clinical research changed over this time period and ranged from innovations in surgical technique to analysis of clinical outcomes, comparative analyses with other reconstructive modalities, timing of reconstruction, and preoperative diagnostic workup, as well as cost-effectiveness analyses. This literature review illustrates the dramatic change that has occurred subsequent to introduction of abdominal flaps for breast reconstruction. While the use of abdominal flaps has become widely accepted for breast reconstruction, many questions remain unanswered, thus highlighting the need for ongoing clinical investigation.},
author = {Suckau, Jan and Arkudas, Andreas and Beier, Justus and Momeni, Arash and Horch, Raymund E. and Cai, Aijia},
doi = {10.12659/MSM.914665},
faupublication = {yes},
journal = {Medical Science Monitor},
note = {CRIS-Team Scopus Importer:2019-06-04},
pages = {3520-3536},
peerreviewed = {Yes},
title = {{Autologous} {Breast} {Reconstruction} with {Transverse} {Rectus} {Abdominis} {Musculocutaneous} ({TRAM}) or {Deep} {Inferior} {Epigastric} {Perforator} ({DIEP}) {Flaps}: {An} {Analysis} of the 100 {Most} {Cited} {Articles}},
volume = {25},
year = {2019}
}
@article{faucris.122078264,
abstract = {New therapeutic strategies are required for critical size bone defects, because the gold standard of transplanting autologous bone from an unharmed area of the body often leads to several severe side effects and disadvantages for the patient. For years, tissue engineering approaches have been seeking a stable, axially vascularized transplantable bone replacement suitable for transplantation into the recipient bed with pre-existing insufficient conditions. For this reason, the arteriovenous loop model was developed and various bone substitutes have been vascularized. However, it has not been possible thus far to engineer a primary stable and axially vascularized transplantable bone substitute. For that purpose, a primary stable silica-embedded nanohydroxyapatite (HA) bone substitute in combination with blood, bone marrow, expanded, or directly retransplanted mesenchymal stem cells, recombinant human bone morphogenetic protein 2 (rhBMP-2), and different carrier materials (fibrin, cell culture medium, autologous serum) was tested subcutaneously for 4 or 12 weeks in the sheep model. Autologous serum lead to an early matrix change during degradation of the bone substitute and formation of new bone tissue. The best results were achieved in the group combining mesenchymal stem cells expanded with 60 ?g/mL rhBMP-2 in autologous serum. Better ingrowth of fibrovascular tissue could be detected in the autologous serum group compared with the control (fibrin). Osteoclastic activity indicating an active bone remodeling process was observed after 4 weeks, particularly in the group with autologous serum and after 12 weeks in every experimental group. This study clearly demonstrates the positive effects of autologous serum in combination with mesenchymal stem cells and rhBMP-2 on bone formation in a primary stable silica-embedded nano-HA bone grafting material in the sheep model. In further experiments, the results will be transferred to the sheep arteriovenous loop model in order to engineer an axially vascularized primary stable bone replacement in clinically relevant size for free transplantation.},
author = {Boos, Anja and Weigand, Annika and Deschler, Gloria and Gerber, Thomas and Arkudas, Andreas and Kneser, Ulrich and Horch, Raymund E. and Beier, Justus},
doi = {10.2147/IJN.S66867},
faupublication = {yes},
journal = {International Journal of Nanomedicine},
note = {EVALuna2:15577},
pages = {5317-39},
peerreviewed = {Yes},
title = {{Autologous} serum improves bone formation in a primary stable silica-embedded nanohydroxyapatite bone substitute in combination with mesenchymal stem cells and {rhBMP}-2 in the sheep model},
volume = {9},
year = {2014}
}
@article{faucris.122510784,
abstract = {The standardized characterization of angiogenesis is crucial in the field of tissue engineering as sufficient blood supply is the limiting factor of mass transfer. However, reliable algorithms that provide a straight forward and observer-independent assessment of new vessel formation are still lacking. We propose an automatic observer-independent quantitative method (including downloadable source code) to analyze vascularization using two-dimensional microscopic images of histological cross-sections and advanced postprocessing, based on a 'positive- and negative-experts' model and a (corrected) nearest neighbour classification, in a vascularized tissue engineering model.An established angioinductive rat arteriovenous loop model was used to compare the new automatic analysis with a common 2D method and a ?CT algorithm. Angiogenesis was observed at three different time points (5, 10 and 15 days).In line with previous results, formation of functional new vessels that arose from the venous graft was evident within the three-dimensional construct and a significant (p < 0.05) increase in vessel count and area was observed over time. The proposed automatic analysis obtained precise values for vessel count and vessel area that were similar to the manually gained data. The algorithm further provided vectorized parameterization of the newly formed vessels for advanced statistical analysis. Compared to the ?CT-based three-dimensional analyses, the presented two-dimensional algorithm was superior in terms of small vessel detection as well as cost and time efficiency.The quantitative evaluation method, using microscopic images of stained histological sections, 'positive- and negative-experts'-based vessel segmentation, and nearest neighbour classification, provides a user-independent and precise but also time- and cost-effective tool for the analysis of vascularized constructs. Our algorithm, which is freely available to the public, outperforms previous approaches especially in terms of unambiguous vessel classification and statistical analyses.},
author = {Weis, Christian and Covi, J. M. and Hilgert, Johannes and Leibig, N. and Arkudas, Andreas and Horch, Raymund E. and Kneser, U. and Schmidt, V. J.},
doi = {10.1111/jmi.12252},
faupublication = {yes},
journal = {Journal of Microscopy},
note = {EVALuna2:15586},
pages = {185-96},
peerreviewed = {Yes},
title = {{Automatic} quantification of angiogenesis in {2D} sections: a precise and timesaving approach},
volume = {259},
year = {2015}
}
@article{faucris.117030804,
abstract = {In tissue engineering cell cultures play a crucial role besides the matrix materials for the end of substituting lost tissue functions. The cell itself is situated at the cross-roads leading to different orders of scale, from molecule to organism and different levels of function, from biochemistry to macrophysiology. Extensive in vitro investigations have dissected a vast amount of cellular phenomena and the role of a number of bioactive substances has been elucidated in the past. Further, recombinant DNA technologies allow modulation of the expression profiles of virtually all kinds of cells. However, issues of vascularization in vivo limit transferability of these observations and restrict upscaling into clinical applications. Novel in vivo models of vascularization have evolved inspired from reconstructive microsurgical concepts and they encompass axial neovascularization by means of vascular induction. This work represents a brief description of latest developments and potential applications of neovascularization and angiogenesis in tissue engineering.
},
author = {Polykandriotis, Elias and Arkudas, Andreas and Horch, Raymund E. and Stürzl, Michael and Kneser, Ulrich},
doi = {10.1111/j.1582-4934.2007.00012.x},
faupublication = {yes},
journal = {Journal of cellular and molecular medicine},
note = {UnivIS-Import:2015-04-14:Pub.2007.med.packli.pchw2.autono},
pages = {6-20},
peerreviewed = {unknown},
title = {{Autonomously} vascularized cellular constructs in tissue engeneering: opening a new perspective for biomedical science},
volume = {11 (1)},
year = {2007}
}
@article{faucris.238462305,
abstract = {In tissue engineering cell cultures play a crucial role besides the matrix materials for the end of substituting lost tissue functions. The cell itself is situated at the cross-roads leading to different orders of scale, from molecule to organism and different levels of function, from biochemistry to macrophysiology. Extensive in vitro investigations have dissected a vast amount of cellular phenomena and the role of a number of bioactive substances has been elucidated in the past. Further, recombinant DNA technologies allow modulation of the expression profiles of virtually all kinds of cells. However, issues of vascularization in vivo limit transferability of these observations and restrict upscaling into clinical applications. Novel in vivo models of vascularization have evolved inspired from reconstructive microsurgical concepts and they encompass axial neovascularization by means of vascular induction. This work represents a brief description of latest developments and potential applications of neovascularization and angiogenesis in tissue engineering.},
author = {Polykandriotis, Elias and Arkudas, Andreas and Horch, Raymund E. and Stürzl, Michael and Kneser, Ulrich},
doi = {10.1111/j.1582-4934.2007.00012.x},
faupublication = {yes},
journal = {Journal of Cellular and Molecular Medicine},
pages = {6-20},
peerreviewed = {Yes},
title = {{Autonomously} vascularized cellular constructs in tissue engineering: opening a new perspective for biomedical science.},
volume = {11},
year = {2007}
}
@article{faucris.120895544,
abstract = {Prevention of perioperative and postoperative complications resulting from surgical oncology in the pelvic region remains a major interdisciplinary challenge. With modern interdisciplinary concepts joining forces of various surgical specialties, tumor resection can be sufficiently carried out with wide margins and the patients benefit from reduced morbidity even in complex situations. As an example chronic fistulation and secretion from the presacral cavity and sinus may result as potential sequelae from intra-abdominal and intrapelvic tumor resection, especially when neoadjuvant multimodal therapies have been applied. This can be prevented by simultaneous transplantation of for example transpelvic vertical rectus abdominis myocutaneous (VRAM) flap transfer, while extensive perineal skin and soft tissue defects may also be simultaneously reconstructed. In cases of malignant soft tissue tumors in the pelvic region a staged surgical procedure can be performed with a period of time between tumor resection and reconstruction. Thus, a histological R0 status can be secured prior to plastic reconstruction surgery in order to increase oncological safety. In cases of postresectional exposition of e. g. pelvic or femoral vessels or intrapelvic and intra-abdominal organs simultaneous flap procedure is mandatory.The reconstructive armamentarium of the plastic surgeon should contain not only pedicled but also free microsurgical flaps so that no compromise in terms of the extent of the oncological resection has to be accepted. At the same time perioperative and postoperative complications may be avoided and the patient quality of life can be preserved even in more complex cases.},
author = {Beier, Justus and Croner, R. S. and Lang, Werner and Arkudas, Andreas and Schmitz, Marweh and Göhl, Jonas and Hohenberger, Werner and Horch, Raymund E.},
doi = {10.1007/s00104-014-2835-6},
faupublication = {yes},
journal = {Chirurg},
note = {EVALuna2:15195},
pages = {242-50},
peerreviewed = {Yes},
title = {{Avoidance} of complications in oncological surgery of the pelvic region : {Combined} oncosurgical and plastic reconstruction measures},
volume = {86},
year = {2015}
}
@article{faucris.238462574,
abstract = {Generation of axially vascularized bioartificial bone might be performed using matrix neovascularization in connection with osteoblast injection. We sought to evaluate whether prevascularization of porous hard matrices using an arteriovenous (AV) loop promotes survival of transplanted osteoblasts. A processed bovine cancellous bone matrix was inserted into the AV loop. Six weeks later, 5 x 10(6) carboxyfluorescein diacetate-stained osteoblasts were injected into the matrix (group A, n = 34). Osteoblast-seeded matrices without prevascularization were implanted subcutaneously as controls (group B, n = 32). Specimens were subjected to histologic, morphometric, and molecular-biological analysis after 1, 4, 8, and 16 weeks. Upon cell injection, matrices were completely vascularized. An intense foreign body reaction was observed in matrices from both groups. Group A was significantly superior to group B in terms of osteoblast survival at any time point. Expression of bone-specific genes was detected in the AV loop group but not in the subcutaneous control. Bone formation was only detectable in 1 long-term animal of group A. This study demonstrates for the first time that axial prevascularization increases the survival of implanted osteoblasts in porous matrices. Matrices with optimized biocompatibility might eventually facilitate generation of axially vascularized bone tissue after injection of osteogenic cells in the AV loop model.},
author = {Arkudas, Andreas and Beier, Justus P. and Heidner, Kristina and Tjiawi, Jimmy and Polykandriotis, Elias and Srour, Safwan and Stürzl, Michael and Horch, Raymund E. and Kneser, Ulrich},
doi = {10.1089/ten.2006.0387},
faupublication = {yes},
journal = {Tissue Engineering -Larchmont},
pages = {1549-60},
peerreviewed = {Yes},
title = {{Axial} prevascularization of porous matrices using an arteriovenous loop promotes survival and differentiation of transplanted autologous osteoblasts.},
volume = {13},
year = {2007}
}
@article{faucris.266500595,
abstract = {Adjuvant radiotherapy in breast cancer patients might enhance complications after autologous breast reconstruction, including thrombosis of the internal mammary artery (IMA) precluding its use as recipient vessel. This case report shows a salvage procedure for thrombosis of the IMA during bilateral autologous breast reconstruction in a 51-year-old patient who had undergone repeated irradiation of the chest wall after Hodgkin's disease and recurrent breast carcinoma of the right side. After mastectomy of the right breast and prophylactic mastectomy of the left breast, the patient desired breast reconstruction with autologous tissue. During simultaneous bilateral breast reconstruction using two deep inferior epigastric perforator (DIEP) flaps anastomosed to the left and right inframammary vessels, arterial anastomosis to the right IMA was not feasible due to arterial thrombosis. A salvage procedure using an interpositional cephalic vein graft as a crossover bypass and a subcutaneous presternal tunnel was used. The inferior epigastric artery of the right DIEP flap was anastomosed to the venous bypass, which was anastomosed to the contralateral IMA. Both flaps healed without any complications during the postoperative course and follow up of 6 months. The presented case shows the feasibility of a cross-over venous bypass procedure during bilateral breast reconstruction as salvage maneuver for the arterial anastomosis after repeated radiation of the unilateral breast.},
author = {Cai, Aijia and Geierlehner, Alexander and Arkudas, Andreas and Horch, Raymund E.},
doi = {10.1002/micr.30839},
faupublication = {yes},
journal = {Microsurgery},
note = {CRIS-Team WoS Importer:2021-11-26},
peerreviewed = {Yes},
title = {{Bilateral} free flap breast reconstruction using venous cross-over bypass to contralateral internal mammary artery for salvaging thrombosed arterial anastomosis in unilateral repeated irradiation of the breast: {A} case report},
year = {2021}
}
@article{faucris.237939951,
abstract = {Lipoedema is a progressive disease, which predominantly affects women. It is characterised by circumferential growth, with increase in fat tissue of the extremities, and can lead to oedema. In contrast, the lymphoedema is defined by a specific lymphatic drainage disorder and can lead to fibrosis of the surrounding connective tissue. While lipoedema is diagnosed through clinical symptoms and diagnostic imaging can usually only be used to rule out comorbidities, lymphatic drainage disorder can be visualised using imaging methods. Ultrasound is a basis diagnostic tool to show retained interstitial fluid in lip-and lymphoedema. Lymphoscintigraphy is considered to be the gold standard to demonstrate a lymphatic drainage disorder, which can be combined with computed tomography. Indocyanine green(ICG) lymphography is a dynamic imaging tool, which is of increasing significance due to its possible intraoperative use. Magnetic resonance imaging (MRI) lymphography has the ability to visualise a lymphoedema and a lymphatic drainage disorder in three dimensions and is therefore considered to be a meaningful supplement. Therapy of lip-and lymphoedema should always be based on a combination of conservative and surgical strategies. While liposuction and other resection procedures can lead to symptom relief in lipedoema or advanced lymphoedema, newer reconstructive pro cedures such as lymph node transplantation or lymphovenous anastomoses are modern techniques to treat lymphoedema. A long lasting volume reduction can be achieved by creating new lymphatic pathways leading to improved quality of life and reduced symptoms.},
author = {Zetzmann, Katharina and Ludolph, Ingo and Horch, Raymund E. and Boos, Anja},
doi = {10.1055/a-1097-9887},
faupublication = {yes},
journal = {Phlebologie},
keywords = {liposuction},
note = {CRIS-Team Scopus Importer:2020-05-02},
pages = {72-78},
peerreviewed = {Yes},
title = {{Bildgebende} {Diagnostik} zur {Therapieplanung} bei {Lip}- und {Lymphödem}},
volume = {49},
year = {2020}
}
@article{faucris.120553664,
abstract = {Bioactive glass (BG) scaffolds are being investigated for bone tissue engineering applications because of their osteoconductive and angiogenic nature. However, to increase the in vivo performance of the scaffold, including enhancing the angiogenetic growth into the scaffolds, some researchers use different modifications of the scaffold including addition of inorganic ionic components to the basic BG composition. In this study, we investigated the in vitro biocompatibility and bioactivity of Cu-doped BG derived scaffolds in either BMSC (bone-marrow derived mesenchymal stem cells)-only culture or co-culture of BMSC and human dermal microvascular endothelial cells (HDMEC). In BMSC-only culture, cells were seeded either directly on the scaffolds (3D or direct culture) or were exposed to ionic dissolution products of the BG scaffolds, kept in permeable cell culture inserts (2D or indirect culture). Though we did not observe any direct osteoinduction of BMSCs by alkaline phosphatase (ALP) assay or by PCR, there was increased vascular endothelial growth factor (VEGF) expression, observed by PCR and ELISA assays. Additionally, the scaffolds showed no toxicity to BMSCs and there were healthy live cells found throughout the scaffold. To analyze further the reasons behind the increased VEGF expression and to exploit the benefits of the finding, we used the indirect method with HDMECs in culture plastic and Cu-doped BG scaffolds with or without BMSCs in cell culture inserts. There was clear observation of increased endothelial markers by both FACS analysis and acetylated LDL (acLDL) uptake assay. Only in presence of Cu-doped BG scaffolds with BMSCs, a high VEGF secretion was demonstrated by ELISA; and typical tubular structures were observed in culture plastics. We conclude that Cu-doped BG scaffolds release Cu, which in turn act on BMSCs to secrete VEGF. This result is of significance for the application of BG scaffolds in bone tissue engineering approaches.},
author = {Rath, Subha N. and Brandl, Andreas and Hiller, Daniel and Hoppe, Alexander and Gbureck, Uwe and Horch, Raymund E. and Boccaccini, Aldo R. and Kneser, Ulrich},
doi = {10.1371/journal.pone.0113319},
faupublication = {yes},
journal = {PLoS ONE},
peerreviewed = {Yes},
title = {{Bioactive} copper-doped glass scaffolds can stimulate endothelial cells in co-culture in combination with mesenchymal stem cells},
volume = {9},
year = {2014}
}
@article{faucris.210308498,
abstract = {The advent of Tissue Engineering (TE) in the early 1990ies was fostered by the increasing need for functional tissue and organ replacement. Classical TE was based on the combination of carrier matrices, cells and growth factors to reconstitute lost or damaged tissue and organs. Despite considerable results in vitro and in experimental settings the lack of early vascularization has hampered its translation into daily clinical practice so far. A new field of research, called "biofabrication" utilizing latest 3D printing technologies aims at hierarchically and spatially incorporating different cells, biomaterials and molecules into a matrix to alleviate a directed maturation of artificial tissue.\nA literature research of the relevant publications regarding biofabrication and bioprinting was performed using the PubMed data base. Relevant papers were selected and evaluated with secondary analysis of specific citations on the bioprinting techniques.\n180 relevant papers containing the key words were identified and evaluated. Basic principles into the developing field of bioprinting technology could be discerned. Key elements comprise the high-throughput assembly of cells and the fabrication of complex and functional hierarchically organized tissue constructs. Five relevant technological principles for bioprinting were identified, such as stereolithography, extrusion-based printing, laser-assisted printing, inkjet-based printing and nano-bioprinting. The different technical methods of 3D printing were found to be associated with various positive but also negative effects on cells and proteins during the printing process. Research efforts in this field obviously aim towards the development of optimizing the so called bioinks and the printing technologies.\nThis review details the evolution of the classical methods of TE in Regenerative Medicine into the evolving field of biofabrication by bioprinting. The advantages of 3D bioprinting over traditional tissue engineering techniques are based on the assembling of cells, biomaterials and biomolecules in a spatially controlled manner to reproduce native tissue macro-, micro- and nanoarchitectures, that can be utilized not only to potentially produce functional replacement tissues or organs but also to serve as new models for basic research. Mimicking the stromal microenvironment of tumor cells to study the process of tumor formation and progression, metastasis, angiogenesis and modulation of the associated processes is one of these applications under research. To this end a close collaboration of specialists from the fields of engineering, biomaterial science, cell biology and reconstructive microsurgery will be necessary to develop future strategies that can overcome current limitations of tissue generation.\nBACKGROUND\nMATERIALS AND METHODS\nRESULTS\nCONCLUSION},
author = {Horch, Raymund E. and Weigand, Annika and Wajant, Harald and Groll, Juergen and Boccaccini, Aldo R. and Arkudas, Andreas},
doi = {10.1055/s-0043-124674},
faupublication = {yes},
journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie},
pages = {93-100},
peerreviewed = {Yes},
title = {{Biofabrication}: new approaches for tissue regeneration},
volume = {50},
year = {2018}
}
@article{faucris.123759944,
abstract = {With the advent of the era of tissue engineering (TE), experimental settings have been developed that allow for a defined environment with optimised cell growth conditions and/or the production of specific substitutes. These isolated systems have been termed "bioreactors". By translating the principles of bioreactors into an in vivo context, advances in biomaterial sciences and cell biology have been merged into an integrative research concept. Even today, in the age of regenerative Medicine (RegMed) the transfer of experimental in vitro findings into a clinical in vivo approach still remains a vast challenge. In order to fulfil these specific requirements bioreactors had to be defined anew. Latest advances in areas like reconstructive medicine (the arteriovenous loop as a means of organogenesis) or modern wound management (topical negative pressure therapy as a perfusion bioreactor) give new impulses towards the translation of RegMed concepts into the clinical routine.},
author = {Polykandriotis, E. and Schmidt, V. J. and Kneser, U. and Jianming, S. and Boccaccini, Aldo R. and Horch, Raymund E.},
doi = {10.1055/s-0032-1321838},
faupublication = {yes},
journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie},
keywords = {angiogenesis;tissue engineering;growth factors;experimental transplantation;bioreactors;negative pressure therapy},
pages = {198-203},
peerreviewed = {Yes},
title = {{Bioreactors} in {Regenerative} {Medicine} - {From} a {Technical} {Device} to a {Reconstructive} {Alternative}?},
volume = {44},
year = {2012}
}
@article{faucris.225557628,
abstract = {Background: The aim of the study was to compare physical activity (PA) in patients who had undergone massive weight loss (MWL), before and after body contouring procedures. Methods: All patients undergoing body contouring surgery after MWL between 2007 and 2017 with a minimum follow-up of 6 months after the last procedure were included in this retrospective study. Excluded were those with a body mass index > 35 kg/m2 and those with comorbidities leading to impaired PA. Quality of life (QOL) was assessed using the Moorehead-Ardelt QOL Questionnaire II. Evaluation of PA was obtained with the International Physical Activity Questionnaire (IPAQ) short form and the Freiburg PA Questionnaire. Functional impairment during exercise was analyzed using a self-designed functional impairment score (FIS). Results: In the 45 patients completing the survey (37 female, 8 male), an improvement in QOL (p < 0.001) and PA scored by the IPAQ (p = 0.017) was found. The Freiburg PA Questionnaire showed no difference in PA before and after body contouring surgery (p = 0,274). Furthermore, scores of the FIS indicated a decrease of functional impairment during physical activity after body contouring surgery (p < 0.001). Conclusion: Body contouring improves QOL and PA in patients after massive weight loss. The results of our study confirm the important role of plastic surgery in the treatment and maintenance of health of patients with former obesity.},
author = {Maringa, Linda and Hauck, Theresa and Boos, Anja and Schmitz, Marweh and Arkudas, Andreas and Horch, Raymund E. and Ludolph, Ingo and Cai, Aijia},
doi = {10.1007/s11695-019-04145-3},
faupublication = {yes},
journal = {Obesity Surgery},
keywords = {Body contouring surgery; Massive weight loss; Physical activity; Quality of life},
note = {CRIS-Team Scopus Importer:2019-09-03},
peerreviewed = {Yes},
title = {{Body} {Contouring} {Surgery} {Improves} {Physical} {Activity} in {Patients} {After} {Massive} {Weight} {Loss}—a {Retrospective} {Study}},
year = {2019}
}
@article{faucris.259304946,
abstract = {Background: Early failure of osteosyntheses is common even with use of locking plates. In patients with comminuted fractures and epiphyseal osseous defects, we performed a series of osteosyntheses by locking plate in combination with an allograft bone augmentation. Because of encouraging short-term results in the literature, we assumed that the method could be a potential alternative to a reverse shoulder prosthesis.},
author = {Polykandriotis, Elias and Zschiegner, Annelie and Horch, Raymund E. and Schürmann, Matthias},
doi = {10.12659/MSM.928982},
faupublication = {yes},
journal = {Medical Science Monitor},
note = {CRIS-Team WoS Importer:2021-05-28},
peerreviewed = {Yes},
title = {{Bone} {Allograft} and {Locking} {Plate} for {Severe} {Proximal} {Humeral} {Fractures}: {Early} and {Late} {Outcomes}},
volume = {27},
year = {2021}
}
@article{faucris.249350333,
abstract = {Bone fractal signature analysis (FSA-also termed bone texture analysis) is a tool that assesses structural changes that may relate to clinical outcomes and functions. Our aim was to compare bone texture analysis of the distal radius in patients and volunteers using radiography and 3T and 7T magnetic resonance imaging (MRI)-a patient group (n = 25) and a volunteer group (n = 25) were included. Participants in the patient group had a history of chronic wrist pain with suspected or confirmed osteoarthritis and/or ligament instability. All participants had 3T and 7T MRI including T1-weighted turbo spin echo (TSE) sequences. The 7T MRI examination included an additional high-resolution (HR) T1 TSE sequence. Radiographs of the wrist were acquired for the patient group. When comparing patients and volunteers (unadjusted for gender and age), we found a statistically significant difference of horizontal and vertical fractal dimensions (FDs) using 7T T1 TSE-HR images in low-resolution mode (horizontal: p = 0.04, vertical: p = 0.01). When comparing radiography to the different MRI sequences, we found a statistically significant difference for low- and high-resolution horizontal FDs between radiography and 3T T1 TSE and 7T T1 TSE-HR. Vertical FDs were significantly different only between radiographs and 3T T1 TSE in the high-resolution mode; FSA measures obtained from 3T and 7T MRI are highly dependent on the sequence and reconstruction resolution used, and thus are not easily comparable between MRI systems and applied sequences.},
author = {Jarraya, Mohamed and Heiß, Rafael and Duryea, Jeffrey and Nagel, Armin Michael and Lynch, John A. and Guermazi, Ali and Weber, Marc-Andre and Arkudas, Andreas and Horch, Raymund E. and Uder, Michael and Roemer, Frank},
doi = {10.3390/diagnostics11010110},
faupublication = {yes},
journal = {Diagnostics},
month = {Jan},
note = {CRIS-Team WoS Importer:2021-02-12},
peerreviewed = {Yes},
title = {{Bone} {Structure} {Analysis} of the {Radius} {Using} {Ultrahigh} {Field} ({7T}) {MRI}: {Relevance} of {Technical} {Parameters} and {Comparison} with {3T} {MRI} and {Radiography}},
volume = {11},
year = {2021}
}
@article{faucris.205082069,
abstract = {For decades, researchers have been developing a range of promising strategies in bone tissue engineering with the aim of producing a significant clinical benefit over existing therapies. However, a major problem concerns the traditional use of xenogeneic substances for the expansion of cells, which complicates direct clinical transfer. The study's aim was to establish a totally autologous sheep model as a basis for further preclinical studies and future clinical application. Ovine mesenchymal stromal cells (MSC) were cultivated in different concentrations (0%, 2%, 5%, 10%, and 25%) of either autologous serum (AS) or fetal calf serum (FCS). With an increase of serum concentration, enhanced metabolic activity and proliferation could be observed. There were minor differences between MSC cultivated in AS or FCS, comparing gene and protein expression of osteogenic and stem cell markers, morphology, and osteogenic differentiation. MSC implanted subcutaneously in the sheep model, together with a nanostructured bone substitute, either in stable block or moldable putty form, induced similar vascularization and remodeling of the bone substitute irrespective of cultivation of MSC in AS or FCS and osteogenic differentiation. The bone substitute in block form together with MSC proved particularly advantageous in the induction of ectopic bone formation compared to the cell-free control and putty form. It could be demonstrated that AS is suitable for replacement of FCS for cultivation of ovine MSC for bone tissue engineering purposes. Substantial progress has been made in the development of a strictly xenogeneic-free preclinical animal model to bring future clinical application of bone tissue engineering strategies within reach.},
author = {Weigand, Annika and Beier, Justus and Schmid, Rafael and Knorr, Thomas and Kilian, David and Goetzl, Rebekka and Gerber, Thomas and Horch, Raymund E. and Boos, Anja},
doi = {10.1089/ten.TEA.2016.0176},
faupublication = {yes},
journal = {Tissue Engineering: Parts A, B, and C},
note = {EVALuna2:32847},
pages = {208-222},
peerreviewed = {Yes},
title = {{Bone} {Tissue} {Engineering} {Under} {Xenogeneic}-{Free} {Conditions} in a {Large} {Animal} {Model} as a {Basis} for {Early} {Clinical} {Applicability}},
volume = {23},
year = {2017}
}
@article{faucris.238596174,
abstract = {The microvascular endothelial network is essential for bone formation and regeneration. In this context, endothelial cells not only support vascularization but also influence bone physiology via cell contact-dependent mechanisms. In order to improve vascularization and osteogenesis in tissue engineering applications, several strategies have been developed. One promising approach is the coapplication of endothelial and adipose derived stem cells (ADSCs). In this study, we aimed at investigating the best ratio of human umbilical vein endothelial cells (HUVECs) and osteogenic differentiated ADSCs with regard to proliferation, apoptosis, osteogenesis and angiogenesis. For this purpose, cocultures of ADSCs and HUVECs with ratios of 25%:75%, 50%:50% and 75%:25% were performed. We were able to prove that cocultivation supports proliferation whereas apoptosis was unidirectional decreased in cocultured HUVECs mediated by a p-BAD-dependent mechanism. Moreover, coculturing ADSCs and HUVECs stimulated matrix mineralization and the activity of alkaline phosphatase (ALP). Increased gene expression of the proangiogenic markers eNOS, Flt, Ang2 and MMP3 as well as sprouting phenomena in matrigel assays proved the angiogenic potential of the coculture. In summary, coculturing ADSCs and HUVECs stimulates proliferation, cell survival, osteogenesis and angiogenesis particularly in the 50%:50% coculture.},
author = {Mutschall, Hilkea and Winkler, Sophie and Weisbach, Volker Günter and Arkudas, Andreas and Horch, Raymund E. and Steiner, Dominik},
doi = {10.1111/jcmm.15374},
faupublication = {yes},
journal = {Journal of Cellular and Molecular Medicine},
keywords = {ADSC; angiogenesis; bone tissue engineering; HUVEC; osteogenesis},
note = {CRIS-Team Scopus Importer:2020-05-22},
peerreviewed = {Yes},
title = {{Bone} tissue engineering using adipose-derived stem cells and endothelial cells: {Effects} of the cell ratio},
year = {2020}
}
@article{faucris.234227940,
abstract = {The most common donor-site for autologous breast reconstruction is the abdomen. Over the past several decades technical advances have resulted in the development of flaps that have been associated with a progressive decrease in abdominal wall morbidity. However, controversy exists related to the differences between muscle-sparing (MS)-TRAM and deep inferior epigastric perforator (DIEP) flaps. Hence, the question which approach should be considered standard of care remains unanswered. To address this question the current literature and published evidence was critically reviewed and discussed by an expert panel at the 39th Annual Meeting of the German-speaking Society for Micro surgery of the Peripheral Nerves and Vessels (DAM). Based on this discussion a consensus statement was developed that incorporates contemporary data regarding postoperative complication rate, donor site morbidity, as well as expert opinion regarding technical details in autologous breast reconstruction with free TRAM and DIEP flaps.},
author = {Eisenhardt, Steffen U. and Momeni, Arash and Von Fritschen, Uwe and Horch, Raymund E. and Starks, G. Bjoern and Bannasch, Holger and Harder, Yves and Heitmann, Christoph and Kremer, Thomas and Rieger, Ulrich M. and Kneser, Ulrich},
doi = {10.1055/a-0631-9025},
faupublication = {yes},
journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie},
note = {EVALuna2:211474},
pages = {248-255},
peerreviewed = {Yes},
title = {{Breast} reconstruction with the free {TRAM} or {DIEP} flap – {What} is the current standard? {Consensus} {Statement} of the {German} {Speaking} {Working} {Group} for {Microsurgery} of the {Peripheral} {Nerves} and {Vessels}},
volume = {50},
year = {2018}
}
@article{faucris.246364191,
abstract = {The fluoride ions of the industrially largely irreplaceable, locally corrosive hydrofluoric acid (HF) can scavenge cations in biological tissues, which explains their high toxic potential, and also leads to local acidification through proton release. The influence of three complexing agents, calcium (Ca2+) gluconate (as 2.5% Ca2+gel and individually (2.84%) or commercially (10%) formulated Ca2+solution), magnesium (Mg2+) gluconate (2.84%) solution and aluminium (Al3+) solution (Hexafluorine®, pure and diluted) on the absorption of fluoride following HF exposure (1–3 min, 100 μl, 30%/0.64 cm2) through human skin was investigated in an ex-vivo diffusion cell model. Fluoride absorption was assessed over 6–24 h and analysed with a fluoride electrode. Decreasing the contamination time reduced the fluoride absorption distinctly which was further reduced by the application of fluoride-binding decontamination agents (Ca2+, Mg2+, Al3+) or water alone without being significantly different. Ca2+ appeared slightly more effective than Mg2+ in reducing fluoride absorption. Moreover, the addition of pH adjusting buffer promoted the decontamination efficacy. Fluoride-binding agents can facilitate the decontamination of dermal HF exposure. However, prompt decontamination appeared to be the key to successful limitation of fluoride absorption and pushes the choice of decontamination agent almost into the background.},
author = {Mini Vijayan, Suvarna and Göen, Thomas and Dennerlein, Kathrin and Horch, Raymund E. and Ludolph, Ingo and Drexler, Hans and Kilo, Sonja},
doi = {10.1016/j.tiv.2020.105055},
faupublication = {yes},
journal = {Toxicology in Vitro},
keywords = {Aluminium; Calcium gluconate; Decontamination; Fluoride absorption; Human skin; Hydrofluoric acid},
note = {CRIS-Team Scopus Importer:2020-12-04},
peerreviewed = {Yes},
title = {{Calcium}, magnesium and aluminium ions as decontaminating agents against dermal fluoride absorption following hydrofluoric acid exposure},
volume = {71},
year = {2021}
}
@article{faucris.113685484,
abstract = {Tissue engineering (TE) has evoked new hopes for the cure of organ failure and tissue loss by creating functional substitutes in the laboratory. Besides various innovations in the context of Regenerative Medicine (RM), TE also provided new technology platforms to study mechanisms of angiogenesis and tumour cell growth as well as potentially tumour cell spreading in cancer research. Recent advances in stem cell technology - including embryonic and adult stem cells and induced pluripotent stem cells - clearly show the need to better understand all relevant mechanisms to control cell growth when such techniques will be administered to patients. Such TE-Cancer research models allow us to investigate the interactions that occur when replicating physiological and pathological conditions during the initial phases of replication, morphogenesis, differentiation and growth under variable given conditions. Tissue microenvironment has been extensively studied in many areas of TE and it plays a crucial role in cell signalling and regulation of normal and malignant cell functions. This article is intended to give an overview on some of the most recent developments and possible applications of TE and RM methods with regard to the improvement of cancer research with TE platforms. The synthesis of TE with innovative methods of molecular biology and stem-cell technology may help investigate and potentially modulate principal phenomena of tumour growth and spreading, as well as tumour-related angiogenesis. In the future, these models have the potential to investigate the optimal materials, culture conditions and material structure to propagate tumour growth. © 2013 The Authors.},
author = {Horch, Raymund E. and Boos, Anja and Yuan, Quan and Bleiziffer, Oliver and Detsch, Rainer and Boccaccini, Aldo R. and Alexiou, Christoph and Sun, Jiaming and Beier, Justus and Arkudas, Andreas},
doi = {10.1111/jcmm.12130},
faupublication = {yes},
journal = {Journal of Cellular and Molecular Medicine},
keywords = {Angiogenesis; AV loop; Cancer research; Cell transplantation; in vitro tumour models; Metastasis; Telocytes; Tissue engineering; Tumour cells; Tumour growth; Tumourgenesis},
note = {UnivIS-Import:2015-03-09:Pub.2013.tech.IW.LM.cancer},
pages = {1197-1206},
peerreviewed = {Yes},
title = {{Cancer} research by means of tissue engineering - is there a rationale?},
year = {2013}
}
@article{faucris.215882757,
abstract = {In this study, we evaluated a new aspect of negative pressure wound therapy (NPWT) as an analytical tool for pharmacokinetic studies. Twenty-one patients with soft tissue defects scheduled to receive NPWT were included in this study. Concomitant to NPWT, all patients received intravenous moxifloxacin (MX). At different time intervals, blood plasma levels of MX were sampled and compared with synchronous concentrations of MX in the exudate obtained from the NPWT drainage system. Serial measurements were performed upon initiation of the therapy as well as in the steady state (after 5 days). At steady state, wound tissue was obtained intraoperatively. High-performance liquid-chromatography (HPLC) was used for analysis. At 1 hour post-administration, the exudate/plasma levels (mg/L) were 1.92/3.07; at 12 hours, 0.80/1.14; at 24 hours, 0.26/0.43; and at 120 hours (steady state), 0.42/0.47. There was a correlation between exudate and plasma levels reaching approximately 0.75. Until now, methods for pharmacokinetic studies concerning interstitial fluid are difficult to apply in the clinical context. The presented method showed limitations, but we believe that, after methodological improvements, measurements of substances in the interstitial fluid by means of NPWT are feasible.},
author = {Polykandriotis, Elias and Horch, Raymund E. and Jost, Matthias and Arkudas, Andreas and Kees, Frieder and Schmitz, Marweh},
doi = {10.1111/iwj.13063},
faupublication = {yes},
journal = {International Wound Journal},
note = {CRIS-Team WoS Importer:2019-04-12},
pages = {503-510},
peerreviewed = {Yes},
title = {{Can} systemically administered antibiotics be detected in wound tissues and surfaces under negative pressure wound therapy?},
volume = {16},
year = {2019}
}
@article{faucris.290417532,
abstract = {To evaluate the feasibility of an iPad-based documented patient briefing for Magnetic Resonance Imaging (MRI) examinations. A standard briefing sheet and questionnaire for a MRI scan was converted from paper form into an iPad application. Twenty patients, who had been referred for an MRI scan, were briefed about the examination in paper form as well as via the iPad application before performing the MRI scan. Time each patient needed for the briefing and the number of questions that came up were documented. Patients' acceptance of the electronic briefing was assessed using a questionnaire. The mean processing time was 2.36 min (range 0.58 to 09.35 min., standard deviation ±2.05 min) for the paper-based briefing and 4.15 min (range 1.56 to 13.48 min, SD ± 2.36 min) for the app-based briefing. Concerning technical aspects, patients asked two questions during the app-based briefing; no questions arose during the paper-based briefing. Six patients preferred electronic briefing and four patients, the paper-based form. No patient preferred the electronic form with additional multimedial information. Eight participants did not mind which briefing version was used; two participants did not express their preference at all. Our experiences showed that electronic briefing using an iPad is feasible and has the potential to become a user-friendly alternative to the conventional paper-based approach. Owing to the broad range of the results, a follow-up study will seek to determine the influencing factors on processing time and other potential questions. © 2012 Society for Imaging Informatics in Medicine.},
author = {Schlechtweg, Philipp and Hammon, Matthias and Heberlein, Christian and Giese, David Andreas and Uder, Michael and Schwab, Siegfried},
doi = {10.1007/s10278-012-9560-9},
faupublication = {yes},
journal = {Journal of Digital Imaging},
keywords = {App; Apple; Electronic patient consent; Feasibility; iPad; Tablet PC},
note = {CRIS-Team Scopus Importer:2023-03-08},
pages = {383-392},
peerreviewed = {Yes},
title = {{Can} the documented patient briefing be carried out with an {iPad} {App}?},
volume = {26},
year = {2013}
}
@article{faucris.205082301,
abstract = {BACKGROUND: Coronary bypass surgery using the internal mammary artery (IMA) is among the most commonly performed procedures in treatment of advanced coronary vessel disease. Further, bilateral harvesting of the IMA is associated with increased rates of sternal wound infections.
OBJECTIVE: This study aimed to explore changes in sternal perfusion, following left internal mammary artery (LIMA) harvesting.
METHODS: 60 patients were divided equally into intervention- and control cohorts and underwent assessment of oxygen saturation (sO2) and relative blood flow (RBF) by laser doppler flowmetry remission spectroscopy preoperatively, 24 h and 72 h postoperatively.
RESULTS: 24 h postoperatively a significant decrease of sO2 and RBF could be detected in LIMA harvest patients. After 72 h this difference disappeared. The side comparison within the groups 24 h postoperatively revealed a reduction of sO2 on the LIMA side within both groups. Regarding the 24 h RBF in side comparison, no significant differences could be detected within the groups. The 72 h side comparison showed an increased RBF for the non-LIMA side within the control group.
CONCLUSIONS: The use of the LIMA may lead to significant decreases in local tissue oxygen saturation and RBF. This reduction is mostly distinct within the first 3 days postoperatively and may influence sternal wound infections.
},
author = {Taeger, Christian and Klein, Konstantin and Horch, Raymund E. and Weyand, Michael and Beier, Justus},
doi = {10.3233/CH-170245},
faupublication = {yes},
journal = {Clinical Hemorheology and Microcirculation},
note = {EVALuna2:32852},
pages = {35-43},
peerreviewed = {Yes},
title = {{Changes} in sternal perfusion following internal mammary artery bypass surgery},
volume = {67},
year = {2017}
}
@article{faucris.224745662,
abstract = {BACKGROUND: Negative pressure wound therapy applied over closed incisions (ciNPT) has been shown to influence wound healing. Therefore, the aim of this study was to examine the short-term effects of ciNPT on skin perfusion patterns in postbariatric wounds.},
author = {Renno, Isabell and Boos, Anja and Horch, Raymund E. and Ludolph, Ingo},
doi = {10.3233/CH-180450},
faupublication = {yes},
journal = {Clinical Hemorheology and Microcirculation},
month = {Jan},
note = {CRIS-Team WoS Importer:2019-08-20},
pages = {139-150},
peerreviewed = {Yes},
title = {{Changes} of perfusion patterns of surgical wounds under application of closed incision negative pressure wound therapy in postbariatric patients},
volume = {72},
year = {2019}
}
@article{faucris.120496684,
abstract = {Vascularisation of scaffolds is now recognised as a crucial requirement for the success of tissue engineering strategies. This review summarises the state-of-the-art in the techniques available for the in vivo assessment of vascularisation of scaffolds with focus on growth factor delivering scaffolds, microfabrication technologies and in vivo characterisation methods based on the arteriovenous loop model to create three dimensionally vascularised tissue replacements.},
author = {Arkudas, Andreas and Beier, Justus and Boccaccini, Aldo R. and Horch, Raymund E.},
doi = {10.1179/1743284714Y.0000000611},
faupublication = {yes},
journal = {Materials Science and Technology},
keywords = {Tissue engineering;AV loop;Cancer research;Regenerative medicine;Reviews},
month = {Jan},
pages = {180-187},
peerreviewed = {Yes},
title = {{Characterisation} of vascularisation of scaffolds for tissue engineering},
volume = {31},
year = {2015}
}
@article{faucris.309908518,
abstract = {Introduction: Neoadjuvant chemotherapy and radiotherapy for the management of soft tissue sarcomas (STS) are still preferably delivered sequentially, with or without concurrent hyperthermia. Concurrent delivery of chemo-, radio- and thermotherapy may produce synergistic effects and reduce chemotherapy-free intervals. The few available studies suggest that concurrent chemoradiation (CRT) has a greater local effect. Data on the efficacy and toxicity of adding hyperthermia to CRT (CRTH) are sparse. Materials and methods: A cohort of 101 patients with STS of the extremities and trunk who received CRT (n = 33) or CRTH (n = 68) before resection of macroscopic tumor (CRT: n = 19, CRTH: n = 49) or re-resection following a non-oncological resection, so called ‘whoops procedure’, (CRT: n = 14, CRTH: n = 19) were included in this retrospective study. CRT consisted of two cycles of doxorubicine (50 mg/m2 on d2) plus ifosfamide (1500 mg/m2 on d1-5, q28) plus radiation doses of up to 60 Gy. Hyperthermia was delivered in two sessions per week. Results: All patients received the minimum dose of 50 Gy. Median doses of ifosfamide and doxorubicin were comparable between CRT (75%/95%) and CRTH (78%/97%). The median number of hyperthermia sessions was seven. There were no differences in acute toxicities. Major wound complications occurred in 15% (CRT) vs. 25% (CRTH) (p = 0.19). In patients with macroscopic disease, the addition of hyperthermia resulted in a tendency toward improved remission: regression ≥90% occurred in 21/48 (CRTH) vs. 4/18 (CRT) patients (p = 0.197). With a median postoperative follow-up of 72 months, 6-year local control and overall survival rates for CRTH vs. CRT alone were 85 vs. 78% (p = 0.938) and 79 vs. 71% (p = 0.215). Conclusions: Both CRT and CRTH are well tolerated with an expected rate of wound complications. The results suggest that adding hyperthermia may improve tumor response.},
author = {Willner, Alexander and Agaimy, Abbas and Fechner, Katja and Ott, Oliver and Denz, Axel and Weissmann, Thomas and Meidenbauer, Norbert and Höfler, Daniel and Gaipl, Udo and Frey, Benjamin and Schmidt, Manfred and Haller, Florian and Horch, Raymund E. and Hartmann, Arndt and Grützmann, Robert and Fietkau, Rainer and Semrau, Sabine},
doi = {10.1080/02656736.2023.2248424},
faupublication = {yes},
journal = {International Journal of Hyperthermia},
keywords = {chemoradiotherapy; chemotherapy; hyperthermia; neoadjuvant; radiotherapy; Sarcoma; soft-tissue sarcoma; toxicity},
note = {CRIS-Team Scopus Importer:2023-09-01},
peerreviewed = {Yes},
title = {{Chemoradiotherapy} plus hyperthermia ({CRTH}) versus chemoradiotherapy ({CRT}) alone in neoadjuvant treatment of soft tissue sarcoma: tumor response, treatment toxicity and disease control},
volume = {40},
year = {2023}
}
@article{faucris.297263003,
abstract = {Background Ultrahigh-field-strength MRI at 7 T may permit superior visualization of noninflammatory wrist pathologic conditions, particularly due to its high signal-to-noise ratio compared with the clinical standard of 3 T, but direct comparison studies are lacking. Purpose To compare the subjective image quality of 3-T and 7-T ultrahigh-field-strength wrist MRI through semiquantitative scoring of multiple joint tissues in a multireader study. Materials and Methods In this prospective study, healthy controls and participants with chronic wrist pain underwent 3-T and 7-T MRI (coronal T1-weighted turbo spin-echo [TSE], coronal fat-suppressed proton-density [PD]-weighted TSE, transversal T2-weighted TSE) on the same day, from July 2018 to June 2019. Images were scored by seven musculoskeletal radiologists. The overall image quality, presence of artifacts, homogeneity of fat suppression, and visualization of cartilage, the triangular fibrocartilage complex (TFCC), and scapholunate and lunotriquetral ligaments were semiquantitatively assessed. Pairwise differences between 3 T and 7 T were assessed using the Wilcoxon signed-rank test. Interreader reliability was determined using the Fleiss kappa. Results In total, 25 healthy controls (mean age, 25 years ± 4 [SD]; 13 women) and 25 participants with chronic wrist pain (mean age, 39 years ± 16; 14 men) were included. Overall image quality (P = .002) and less presence of artifacts at PD-weighted fat-suppressed MRI were superior at 7 T. T1- and T2-weighted MRI were superior at 3 T (both P < .001), as was fat suppression (P < .001). Visualization of cartilage was superior at 7 T (P < .001), while visualization of the TFCC (P < .001) and scapholunate (P = .048) and lunotriquetral (P = .04) ligaments was superior at 3 T. Interreader reliability showed slight to substantial agreement for the detected pathologic conditions (κ = 0.20-0.64). Conclusion A 7-T MRI of the wrist had potential advantages over 3-T MRI, particularly in cartilage assessment. However, superiority was not shown for all parameters; for example, visualization of the triangular fibrocartilage complex and wrist ligaments was superior at 3 T. © RSNA, 2023 Supplemental material is available for this article.},
author = {Heiß, Rafael and Weber, Marc-Andre and Balbach, Eva and Schmitt, Rainer and Rehnitz, Christoph and Laqmani, Azien and Sternberg, Andreas and Ellermann, Jutta J. and Nagel, Armin Michael and Ladd, Mark E. and Englbrecht, Matthias and Arkudas, Andreas and Horch, Raymund E. and Guermazi, Ali and Uder, Michael and Roemer, Frank},
doi = {10.1148/radiol.220753},
faupublication = {yes},
journal = {Radiology},
note = {CRIS-Team Scopus Importer:2023-04-21},
pages = {e220753-},
peerreviewed = {Yes},
title = {{Clinical} {Application} of {Ultrahigh}-{Field}-{Strength} {Wrist} {MRI}: {A} {Multireader} 3-{T} and 7-{T} {Comparison} {Study}},
volume = {307},
year = {2023}
}
@article{faucris.106771764,
abstract = {Integrating bioartificial tissues into the host vasculature is a prerequisite for tissue engineering applications. Endothelial progenitor cells (EPCs) display a high angiogenic potential and a low donor-site morbidity, making them ideal for tissue engineering applications. In our study we used a murine EPC cell line (T17b) and rat mesenchymal stem cells (MSCs) for cocultivation experiments. MSCs were cocultured with increasing T17b EPC amounts. Furthermore, MSCs in monoculture were treated with conditioned medium (CM) from T17b EPCs and T17b EPCs were treated with CM from MSCs. Proliferation and apoptosis were quantified with a bromodeoxyuridine ELISA and a DNA fragmentation ELISA, respectively. Osteogenic differentiation was detected with an alkaline phosphatase assay and bone morphogenetic protein-2 ELISA. The production of proangiogenic molecules was measured with a matrix metalloproteinase-3 and vascular endothelial growth factor ELISA as well as nitric oxide assay. We could show that T17b EPCs stimulated MSC proliferation but not vice versa. On the other hand, MSCs promoted the cell survival of EPCs. The growth-inducing and antiapoptotic effects were dependent on heterotypic cell contacts and paracrine mediators. Moreover, proangiogenic growth factors were found in the coculture. Collectively, our results indicate that the coapplication of MSCs and T17b EPCs provides new perspectives for tissue engineering applications.},
author = {Steiner, Dominik and Köhn, Katrin and Beier, Justus and Stürzl, Michael and Horch, Raymund E. and Arkudas, Andreas},
doi = {10.1159/000478654},
faupublication = {yes},
journal = {Cells Tissues Organs},
note = {EVALuna2:15306},
pages = {218-227},
peerreviewed = {Yes},
title = {{Cocultivation} of {Mesenchymal} {Stem} {Cells} and {Endothelial} {Progenitor} {Cells} {Reveals} {Antiapoptotic} and {Proangiogenic} {Effects}},
volume = {204},
year = {2017}
}
@article{faucris.121252604,
abstract = {BACKGROUND: In this study, the authors present a modification of the arteriovenous loop model that combines extrinsic and intrinsic vascularization modes to enhance vascularization of bioartificial matrices. METHODS: An arteriovenous loop was created in the medial thighs of 24 rats. The loop was placed in a newly developed titanium chamber, which was fabricated with an electron beam melting facility, and was embedded in a hydroxyapatite/β- tricalcium phosphate/fibrin matrix. At the explantation time points (2, 4, 6, and 8 weeks), constructs were perfused by differently colored dyes to determine the amount of tissue vascularized by either the intrinsic or the extrinsic vascular pathway. Specimens were investigated by means of micro-computed tomography and histologic and morphometric analysis. RESULTS: Although there was an equal number of blood vessels originating from the center and the periphery, 83 percent of all vessels displayed a connection to the arteriovenous loop already at 2 weeks. There was a continuous increase of the relative proportion of vessels connected to the arteriovenous loop over time detectable. At 8 weeks, communications between the newly formed vessels and the arteriovenous loop were visible in 97 percent of all vessels. CONCLUSIONS: This study demonstrates for the first time the enhancement of angiogenesis in an axially vascularized tissue by an additional extrinsic vascular pathway. By 2 weeks, both pathways showed connections, allowing transplantation of the entire construct using the arteriovenous loop pedicle. This approach will allow for reduction of the time interval between arteriovenous loop implantation and transplantation into the defect site and limitation of operative interventions. Copyright © 2011 by the American Society of Plastic Surgeons.},
author = {Arkudas, Andreas and Galyna, Pryymachuk and Beier, Justus and Weigel, Linda and Körner, Carolin and Singer, Robert and Bleiziffer, Oliver and Polykandriotis, Elias and Horch, Raymund E. and Kneser, Ulrich},
doi = {10.1097/PRS.0b013e3182361f97},
faupublication = {yes},
journal = {Plastic and Reconstructive Surgery},
pages = {55e-65e},
peerreviewed = {Yes},
title = {{Combination} of extrinsic and intrinsic pathways significantly accelerates axial vascularization of bioartificial tissues},
volume = {129},
year = {2012}
}
@article{faucris.110999944,
abstract = {Various local flaps have been described for patients suffering from sternal osteomyelitis and are regarded as a standard procedure for defect reconstruction. In contrast, the use of microsurgical free flaps has been reported anecdotally only. We present our experiences with the combination of an arteriovenous loop and a free flap transfer in defects larger than half of the sternum.Between January 2012 and October 2014, eight patients with a mean age of 68 years who suffered from extensive deep sternal osteomyelitis were operated in a consecutive series. After radical surgical debridement, the defect size comprised all three thirds of the sternum. Defect reconstruction was achieved by means of free flap transplantation using the vastus lateralis myocutaneous flap, rectus abdominis, and bipedicled latissimus dorsi/parascapular flap. As local recipient vessels were depleted in all patients, the pedicle of the flap was anastomosed to a high-flow and short-length subclavian arteriovenous loop as recipient vessel in all cases.The immediate postoperative course in all patients was uneventful despite one axonal injury to the femoral nerve at the donor site. There was no partial or total flap loss. During a mean follow-up of 9 months, one patient suffered from a local infection at the donor site. In all patients, a successful defect reconstruction could be achieved.Depletion of local recipient vessels as an obstacle for free flap reconstruction can be overcome by creating an arteriovenous loop. Even extensive defects are adequate for defect reconstruction using a single or, in extreme cases, bipedicled free flap. © 2015 Wiley Periodicals, Inc. Microsurgery 36:121-127, 2016.},
author = {Taeger, Christian and Horch, Raymund E. and Arkudas, Andreas and Schmitz, Marweh and Stübinger, Axel and Lang, Werner and Meyer, Alexander and Seitz, Tatjana and Weyand, Michael and Beier, Justus},
doi = {10.1002/micr.22405},
faupublication = {yes},
journal = {Microsurgery},
note = {EVALuna2:15222},
pages = {121-7},
peerreviewed = {Yes},
title = {{Combined} free flaps with arteriovenous loops for reconstruction of extensive thoracic defects after sternal osteomyelitis},
volume = {36},
year = {2016}
}
@article{faucris.268293462,
abstract = {Sufficient wound closure of large soft tissue defects remains a challenge for reconstructive surgeons. We aimed to investigate whether combined perforator propeller flaps (PPFs) are suitable to expand reconstructive options. Patients undergoing PPF reconstruction surgery between 2008 and 2021 were screened and evaluated retrospectively. Of 86 identified patients, 69 patients received one perforator propeller flap, while 17 patients underwent combined PPF reconstruction with multiple flaps. We chose major complications as our primary outcome and defined those as complications that required additional surgery. Postoperatively, 27 patients (31.4%) suffered major complications. The propeller flap size, the type of intervention as well as the operation time were not associated with a higher risk of major complications. A defect size larger than 100 cm2, however, was identified as a significant risk factor for major complications among single PPFs but not among combined PPFs (OR: 2.82, 95% CI: 1.01−8.36; p = 0.05 vs. OR: 0.30, 95% CI: 0.02−3.37; p = 0.32). In conclusion, combined PPFs proved to be a reliable technique and should be preferred over single PPFs in the reconstruction of large soft tissue defects at the trunk and proximal lower extremity.},
author = {Bigdeli, Amir K. and Didzun, Oliver and Thomas, Benjamin and Harhaus, Leila and Gazyakan, Emre and Horch, Raymund E. and Kneser, Ulrich},
doi = {10.3390/jpm12010041},
faupublication = {yes},
journal = {Journal of Personalized Medicine},
keywords = {Combined perforator propeller flap; Microsurgery; Perforator flap; Perforator propeller flap; Propeller flap; Soft tissue reconstruction},
month = {Jan},
note = {CRIS-Team Scopus Importer:2022-01-21},
peerreviewed = {Yes},
title = {{Combined} versus {Single} {Perforator} {Propeller} {Flaps} for {Reconstruction} of {Large} {Soft} {Tissue} {Defects}: {A} {Retrospective} {Clinical} {Study}},
volume = {12},
year = {2022}
}
@article{faucris.217016489,
author = {Ludolph, Ingo and Horch, Raymund E.},
doi = {10.1016/j.bjps.2018.11.025},
faupublication = {yes},
journal = {Journal of Plastic Reconstructive and Aesthetic Surgery},
note = {CRIS-Team WoS Importer:2019-05-07},
pages = {778-780},
peerreviewed = {unknown},
title = {{Commentary} on {JPRAS}-{D}-18-00185: {The} use of {Laser} {Speckle} {Contrast} {Imaging} to predict flap necrosis: {An} experimental study in a porcine flap model article},
volume = {72},
year = {2019}
}
@article{faucris.233864397,
author = {Horch, Raymund E.},
doi = {10.1016/j.bjps.2018.02.008},
faupublication = {yes},
journal = {Journal of Plastic Reconstructive and Aesthetic Surgery},
note = {EVALuna2:211478},
pages = {710-711},
peerreviewed = {No},
title = {{Commentary} on the paper: "{Efficacy} of a novel strategy for poststernotomy deep sternal infection after thoracic aorta replacement using a prosthetic graft"},
volume = {71},
year = {2018}
}
@article{faucris.234229423,
author = {Lehnhardt, Marcus and Kneser, Ulrich and Giunta, Riccardo and Horch, Raymund E.},
doi = {10.1055/s-0043-125112},
faupublication = {yes},
journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie},
note = {EVALuna2:154072},
pages = {140-141},
peerreviewed = {No},
title = {{Commentary} on the {Paper}: {The} {Humboldtian} model in {Plastic} and {Reconstructive} {Surgery} - a student survey on teaching and venia legendi},
volume = {50},
year = {2018}
}
@article{faucris.111894244,
author = {Horch, Raymund E.},
doi = {10.1016/j.bjps.2013.12.055},
faupublication = {yes},
journal = {Journal of Plastic Reconstructive and Aesthetic Surgery},
note = {EVALuna2:15561},
pages = {548-9},
peerreviewed = {No},
title = {{Commentary} to '{An} algorithmic approach to post traumatic nail deformities based on an anatomical classification'},
volume = {67},
year = {2014}
}
@article{faucris.123409484,
author = {Weigand, Annika and Horch, Raymund E. and Beier, Justus and Arkudas, Andreas and Boos, Anja},
doi = {10.1111/iwj.12556},
faupublication = {yes},
journal = {International Wound Journal},
note = {EVALuna2:15604},
pages = {1080-2},
peerreviewed = {No},
title = {{Comment} on '{Basic} concepts regarding fractures healing and the current options and future directions in managing bone fractures'},
volume = {13},
year = {2016}
}
@article{faucris.109890264,
author = {Weigand, Annika and Beier, Justus and Arkudas, Andreas and Horch, Raymund E. and Boos, Anja},
doi = {10.1155/2015/685412},
faupublication = {yes},
journal = {BioMed Research International},
note = {EVALuna2:15590},
pages = {685412},
peerreviewed = {unknown},
title = {{Comment} on "{Microsurgical} {Techniques} {Used} to {Construct} the {Vascularized} and {Neurotized} {Tissue} {Engineered} {Bone}"},
volume = {2015},
year = {2015}
}
@article{faucris.234228495,
abstract = {The presented study provides an overview about fingertip injuries and the use of artificial dermis for wound coverage. In specialized centers fingertip injuries are extremely common, independent of the patient ' s age and sex. In the real world probably a considerable number of unreported cases are not referred to a hand surgeon, because such injuries are often misjudged as a minor trauma. Due to this fact, an easy to learn and widely available surgical treatment would be useful. As a result of inadequate treatment beside cosmetic aspects true functional sequelae may occur negatively influencing patients quality of life up to temporary disability or even invalidity. We appreciate the authors conclusion that the complete treatment should be performed by a specialist whenever possible.},
author = {Ludolph, Ingo and Horch, Raymund E.},
doi = {10.1055/a-0580-0155},
faupublication = {yes},
journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie},
note = {EVALuna2:211475},
pages = {276-276},
peerreviewed = {No},
title = {{Comment} on: {One}-stage wound healing of fingertip injury induced by treatment of artificial dermis},
volume = {50},
year = {2018}
}
@article{faucris.204501387,
author = {Horch, Raymund E.},
doi = {10.1055/s-0043-117606},
faupublication = {yes},
journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie},
note = {EVALuna2:32838},
pages = {245-246},
peerreviewed = {Yes},
title = {{Comment} on the {Article}: {Arbitration} processes after mammary augmentation plasties - analysis of procedures with medical malpractice and litigation at the {North} {German} {Arbitration} {Board}},
volume = {49},
year = {2017}
}
@article{faucris.107392384,
author = {Weigand, Annika and Boos, Anja and Beier, Justus and Horch, Raymund E.},
doi = {10.1089/ten.TEA.2013.0658},
faupublication = {yes},
journal = {Tissue Engineering: Parts A, B, and C},
note = {EVALuna2:15563},
pages = {1778-9},
peerreviewed = {Yes},
title = {{Comments} on: "{In} vivo bone regeneration using tubular perfusion system bioreactor cultured nanofibrous scaffolds" vascularization--one challenge of tissue engineering},
volume = {20},
year = {2014}
}
@article{faucris.212286068,
abstract = {INTRODUCTION: Different declotting techniques are available for acutely thrombosed arteriovenous hemodialysis fistulae (AVF). The aim of this single-center study was to compare immediate and follow-up results between surgical repair and a modified interventional lyse-and-wait technique characterized by prolonged local reaction times of recombinant tissue-plasminogen activator (rtPA).
MATERIALS AND METHODS: We retrospectively analyzed medical records of 110 consecutively treated patients between the years 2003 and 2013. All patients were on hemodialysis (HD) and suffered from an acutely thrombosed AVF. The treatment standards changed in the course of time, wherefore we compared 2 groups: the open repair (OR) (N = 50) and the endovascular repair (ER) group (N = 60).
FINDINGS: We found no significant differences in success rates (OR 92%/ER 96.7%; P = 0.28), immediate failures (IFs) (OR 8%/ER 10%; P = 0.71), and temporary catheter placements (TCPs) (OR 10%/ER 6.7%; P = 0.52). Furthermore, there were no differences in cumulative primary patency rates (P = 0.42) and secondary patency rates (P = 0.97).The infection rate was significantly increased after OR (8%) compared to ER (0%); P = 0.026. The hospital stay in days was shorter after ER (5.2 ±2.8) vs. OR (9.0 ± 3.5); P < 0.001.
CONCLUSION: The modified lyse-and-wait technique with prolonged local reaction times is a successful alternative to surgical repair for acutely thrombosed AVF. Clear benefits are less infections and significant shorter hospital stays after ER. However, OR remains the preferred treatment for aneurysms and anastomotic stenoses.},
author = {Regus, Susanne and Almási-Sperling, Veronika and Rother, Ulrich and Meyer, Alexander and Schmid, Axel and Uder, Michael and Lang, Werner},
doi = {10.1111/hdi.12660},
faupublication = {yes},
journal = {Hemodialysis International},
note = {EVALuna2:36234},
pages = {445-453},
peerreviewed = {Yes},
title = {{Comparison} between open and pharmacomechanical repair of acutely thrombosed arteriovenous hemodialysis fistulae within a decade},
volume = {22},
year = {2018}
}
@article{faucris.241985972,
abstract = {Bioprinting offers the opportunity to fabricate precise 3D tumor models to study tumor pathophysiology and progression. However, the choice of the bioink used is important. In this study, cell behavior was studied in three mechanically and biologically different hydrogels (alginate, alginate dialdehyde crosslinked with gelatin (ADA–GEL), and thiol-modified hyaluronan (HA-SH crosslinked with PEGDA)) with cells from breast cancer (MDA-MB-231 and MCF-7) and melanoma (Mel Im and MV3), by analyzing survival, growth, and the amount of metabolically active, living cells via WST-8 labeling. Material characteristics were analyzed by dynamic mechanical analysis. Cell lines revealed significantly increased cell numbers in low-percentage alginate and HA-SH from day 1 to 14, while only Mel Im also revealed an increase in ADA–GEL. MCF-7 showed a preference for 1% alginate. Melanoma cells tended to proliferate better in ADA– GEL and HA-SH than mammary carcinoma cells. In 1% alginate, breast cancer cells showed equally good proliferation compared to melanoma cell lines. A smaller area was colonized in high-percentage alginate-based hydrogels. Moreover, 3% alginate was the stiffest material, and 2.5% ADA–GEL was the softest material. The other hydrogels were in the same range in between. Therefore, cellular responses were not only stiffness-dependent. With 1% alginate and HA-SH, we identified matrices that enable proliferation of all tested tumor cell lines while maintaining expected tumor heterogeneity. By adapting hydrogels, differences could be accentuated. This opens up the possibility of understanding and analyzing tumor heterogeneity by biofabrication.},
author = {Schmid, Rafael and Schmidt, Sonja and Hazur, Jonas and Detsch, Rainer and Maurer, Evelyn and Boccaccini, Aldo R. and Hauptstein, Julia and Teßmar, Jörg and Blunk, Torsten and Schrüfer, Stefan and Schubert, Dirk W. and Horch, Raymund E. and Boßerhoff, Anja Katrin and Arkudas, Andreas and Kengelbach-Weigand, Annika},
doi = {10.3390/cancers12082320},
faupublication = {yes},
journal = {Cancers},
keywords = {Biofabrication; Breast cancer; Hydrogel; Melanoma; Tumor heterogeneity},
note = {CRIS-Team Scopus Importer:2020-08-28},
pages = {1-21},
peerreviewed = {Yes},
title = {{Comparison} of hydrogels for the development of well-defined 3d cancer models of breast cancer and melanoma},
volume = {12},
year = {2020}
}
@article{faucris.308930180,
abstract = {Biomaterials with characteristics similar to extracellular matrix and with suitable bioprinting properties are essential for vascular tissue engineering. In search for suitable biomaterials, this study investigated the three hydrogels alginate/hyaluronic acid/gelatin (Alg/HA/Gel), pre-crosslinked alginate di-aldehyde with gelatin (ADA-GEL), and gelatin methacryloyl (GelMA) with respect to their mechanical properties and to the survival, migration, and proliferation of human umbilical vein endothelial cells (HUVECs). In addition, the behavior of HUVECs was compared with their behavior in Matrigel. For this purpose, HUVECs were mixed with the inks both as single cells and as cell spheroids and printed using extrusion-based bioprinting. Good printability with shape fidelity was determined for all inks. The rheological measurements demonstrated the gelling consistency of the inks and shear-thinning behavior. Different Young’s moduli of the hydrogels were determined. However, all measured values where within the range defined in the literature, leading to migration and sprouting, as well as reconciling migration with adhesion. Cell survival and proliferation in ADA-GEL and GelMA hydrogels were demonstrated for 14 days. In the Alg/HA/Gel bioink, cell death occurred within 7 days for single cells. Sprouting and migration of the HUVEC spheroids were observed in ADA-GEL and GelMA. Similar behavior of the spheroids was seen in Matrigel. In contrast, the spheroids in the Alg/HA/Gel ink died over the time studied. It has been shown that Alg/HA/Gel does not provide a good environment for long-term survival of HUVECs. In conclusion, ADA-GEL and GelMA are promising inks for vascular tissue engineering.},
author = {Schulik, Jana and Salehi, Sahar and Boccaccini, Aldo R. and Schrüfer, Stefan and Schubert, Dirk W. and Arkudas, Andreas and Kengelbach-Weigand, Annika and Horch, Raymund E. and Schmid, Rafael},
doi = {10.3390/bioengineering10070751},
faupublication = {yes},
journal = {Bioengineering},
keywords = {biofabrication; bioprinting; endothelial cells; hydrogels; tissue engineering; vascularization},
note = {CRIS-Team Scopus Importer:2023-08-11},
peerreviewed = {Yes},
title = {{Comparison} of the {Behavior} of {3D}-{Printed} {Endothelial} {Cells} in {Different} {Bioinks}},
volume = {10},
year = {2023}
}
@article{faucris.111439944,
abstract = {To compare the Ramirez technique for the operative closure of large open myelomeningocele defects with conventional closure techniques in newborns. We hypothesized that the immediate surgical treatment with the Ramirez technique is superior to prior used operative techniques.From 2003 to 2010, 23 children (8 female, 15 male) underwent closure of large open myelomeningocele defects using the Ramirez technique (group A), while from 1993 to 2002, 23 children (6 female, 17 male) underwent conventional closure techniques (group B). All children were included in the retrospective analysis with a mean follow-up period of 3.4 years.Perioperative variables were similar in both groups (P = ns). There were no hospital deaths in both groups. The operation time was significantly higher in group A (228.7 ± 76.8 versus 157.8 ± 70.3 min, P = 0.003). Mean length of hospital stay was significantly lower in group A (30.7 ± 16.4 days versus 52.0 ± 38.5; P = 0.02). Postoperative complication rate was significantly lower in group A (P = 0.01). Beyond postoperative day 10, liquor fluid leakage was significantly lower in group A (P = 0.05). During follow-up, there were no complications in group A. In group B, 2 children developed liquor fistulas.The Ramirez technique allows efficient and safe closure of large open myelomeningocele defects and reduces incidence of postoperative liquor fistulae. The increased operation time and surgical efforts seem to be justified. Treatment of large myelomeningocele defects requires an interdisciplinary team including paediatrician-neonatologists, neurosurgeons and plastic surgeons.},
author = {Kneser, Ulrich and Bigdeli, Amir K. and Himmler, Joerg P. and Eyüpoglu, Ilker Yasin and Ganslandt, Oliver Thomas and Hirsch, Almut and Schmidt, Volker J. and Beier, Justus and Horch, Raymund E.},
doi = {10.1016/j.bjps.2015.08.002},
faupublication = {yes},
journal = {Journal of Plastic Reconstructive and Aesthetic Surgery},
note = {EVALuna2:15600},
pages = {1675-82},
peerreviewed = {Yes},
title = {{Comparison} of the {Ramirez} technique for the closure of large open myelomeningocele defects with alternative methods},
volume = {68},
year = {2015}
}
@article{faucris.284512332,
abstract = {Irrespective of numerous technical developments, lymphadenectomy remains a necessary component of surgical tumor therapy. Depending on the extent and anatomical localization, complications associated with the lymph vessels such as lymphoceles, lymphatic fistulas or secondary lymphedema can occur with varying frequency, despite a meticulous dissection technique. Chronic lymph fistulas or lymphoceles often require interventional or surgical procedures. Pedicled or free microsurgical flaps are often required in the case of coexisting wound healing disorders or skin soft tissue defects, especially in an irradiated area. For secondary lymphedema a number of conservative and surgical treatment methods have been established. Adequate guideline-based conservative treatment is the method of first choice. If this does not lead to the desired result, microsurgical reconstructive, deviating or resecting procedures are available.},
author = {Ludolph, Ingo and Arkudas, Andreas and Müller-Seubert, Wibke and Cai, Aijia and Horch, Raymund E.},
doi = {10.1007/s00104-022-01736-2},
faupublication = {yes},
journal = {Die Chirurgie},
note = {CRIS-Team WoS Importer:2022-11-04},
peerreviewed = {Yes},
title = {{Complications} and their management following axillary, inguinal and iliac lymph node dissection},
year = {2022}
}
@article{faucris.107108364,
abstract = {
The mechanical behavior of gel-filled silicone breast
implants has been examined as well as the mechanical properties of the implant
shell itself. For the first
time the implants were
tested under compression in two different ways. Subsequently the implant shell
material was analyzed by tensile test on dumbbell-shaped specimens. The results
are presented in contour plots which provide a detailed view of the measured
values of the samples. Finally a statistical failure analysis was carried out by
means of a Weibull fit to provide further data on the mechanical behavio},
author = {Schubert, Dirk W. and von Hanstein, Harald and Daenicke, Jonas and Werner, Siegfried and Horch, Raymund E.},
doi = {10.1002/pi.5516},
faupublication = {yes},
journal = {Polymer International},
keywords = {Compression test; cyclic loading; mechanical properties; silicone breast implant; tensile test},
pages = {380-385},
peerreviewed = {Yes},
title = {{Compressive} and cyclic loading of silicone breast implants and their effect on shape resilience and reliability of the shell material},
volume = {67},
year = {2018}
}
@article{faucris.113854444,
abstract = {To evaluate the performance of computer-aided evaluation software for a comprehensive workup of patients prior to transcatheter aortic valve implantation (TAVI) using low-contrast agent and low radiation dose third-generation dual-source CT angiography.We evaluated 30 consecutive patients scheduled for TAVI. All patients underwent ECG-triggered high-pitch dual-source CT angiography of the aortic root and aorta with a standardized contrast agent volume (30 ml Imeron350, flow rate 4 ml/s) and low-dose (100 kv/350 mAs) protocol. An expert (10 years of experience) manually evaluated aortic root and iliac access dimensions (distance between coronary ostia and aortic annulus, minimal/maximal diameters and area-derived diameter of the aortic annulus) and best CT-predicted fluoroscopic projection angle as the reference standard. Utilizing computer-aided software (syngo.via), the same pre-TAVI workup was performed and compared to the reference standard.Mean CTDI[Formula: see text] was 3.46 mGy and mean DLP 217.6 ± 12.1 mGy cm, corresponding to a mean effective dose of 3.7 ± 0.2 mSv. Computer-aided evaluation was successful in all but one patient. Compared to the reference standard, Bland-Altman analysis indicated very good agreement for the distances between aortic annulus and coronary ostia (RCA: mean difference 0.8 mm; 95 % CI 0.4-1.2 mm; LM: mean difference 0.9 mm; 95 % CI 0.5-1.3 mm); however, we demonstrated a systematic overestimation of annulus- derived diameter using the software (mean difference 44.4 mm[Formula: see text]; 95 % CI 30.4-58.3 mm[Formula: see text]). Based on respective annulus dimensions, the recommended prosthesis size (Edwards SAPIEN 3) matched in 26 out of the 29 patients (90 %). CT-derived fluoroscopic projection angles showed an excellent agreement for both methods. Out of 58 iliac arteries, 15 (25 %) arteries could not be segmented by the software. Preprocessing time of the software was 71 ± 11 s (range 51-96 s), and reading time with the software was 118 ± 31 s (range 68-201 s).In the workup of pre-TAVI CT angiography, computer-aided evaluation of low-contrast, low-dose examinations is feasible with good agreement and quick reading time. However, a systematic overestimation of the aortic annulus area is observed.},
author = {Dankerl, Peter and Hammon, Matthias and Seuß, Hannes and Tröbs, Monique and Schuhbaeck, Annika and Hell, Michaela M. and Achenbach, Stephan and Uder, Michael and Marwan, Mohamed and Cavallaro, Alexander Josef},
doi = {10.1007/s11548-016-1470-8},
faupublication = {yes},
journal = {International Journal of Computer Assisted Radiology and Surgery},
note = {EVALuna2:14602},
peerreviewed = {Yes},
title = {{Computer}-aided evaluation of low-dose and low-contrast agent third-generation dual-source {CT} angiography prior to transcatheter aortic valve implantation ({TAVI})},
year = {2016}
}
@article{faucris.117872524,
abstract = {Interdisciplinary obesity centers are medically required for the successful treatment of obese patients. Due to the dramatic increase in the number of obese patients needing treatment, it is important to develop interdisciplinary therapy approaches based on clinically and scientifically based knowledge with high levels of evidence. The recent S3 guideline "Surgery of the obese" represents such an evidence-based interdisciplinary approach. In this concept, postbariatric plastic surgery has also been integrated. This article presents an evidence-based therapy algorithm that was developed.},
author = {Dragu, A. and Horch, Raymund E.},
doi = {10.1007/s00104-013-2628-3},
faupublication = {yes},
journal = {Chirurg},
note = {EVALuna2:15557},
pages = {37-41},
peerreviewed = {Yes},
title = {{Concept} of reconstructive body shaping in obesity. {Evidence}-based therapy algorithm},
volume = {85},
year = {2014}
}
@article{faucris.204503541,
abstract = {On occasion of the Munich Plastic Symposium in Munich the board of the Deutsche Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen (DGPRÄC) together with a group of experts who were also involved in the preparation of the recently published S2K guideline "Autologous Fat Grafting", prepared a consensus statement from a plastic-surgical point of view so to evaluate current spects and taking into account the current legal framework: 1. Autologous Fat Grafting is a long established treatment in plastic surgery and does not differ from other tissue grafts. 2. Mechanical processing of autologous fat does not provide any substantial change tot he tissue. 3. If other treatment methods to enrich progenitor cells of autolous fat i. e. by an enzymatic process have evidence that autologous adipose tissue or cells were substantially changed, classification as a drug could come in question under current german law (application of AMG/ATMP).
},
author = {Giunta, R. E. and Horch, Raymund E. and Prantl, L. and Baur, E. M. and Herold, C. and Kamolz, Lars-Peter and Lehnhardt, M. and Noah, E. M. and Rennekampff, O. and Richter, D. and Schaefer, D. J. and Ueberreiter, K.},
doi = {10.1055/s-0042-121893},
faupublication = {yes},
journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie},
note = {EVALuna2:32823},
pages = {337-339},
peerreviewed = {Yes},
title = {{Consensus} of the {Deutsche} {Gesellschaft} der {Plastischen}, {Rekonstruktiven} und {Ästhetischen} {Chirurgen} ({DGPRÄC}) on {Autologous} {Fat} {Grafting}},
volume = {48},
year = {2016}
}
@article{faucris.275215441,
author = {Horch, Raymund E. and Braumann, Chris and Dissemond, Joachim and Lehner, Burkhard and Hirche, Christoph and Woeste, Guido and Wozniak, Gernold and Wetzel-Roth, Walter and Willy, Christian},
doi = {10.1055/a-0881-4595},
faupublication = {yes},
journal = {Zentralblatt für Chirurgie},
note = {EVALuna2:219447},
pages = {152-152},
peerreviewed = {Yes},
title = {[{Correction}: {Use} of {Negative} {Pressure} {Wound} {Therapy} with {Instillation} and {Dwell} {Time} for {Wound} {Treatment} - {Results} of an {Expert} {Consensus} {Conference}].},
volume = {144},
year = {2019}
}
@article{faucris.289684340,
abstract = {Incorrect Author Name In the published article, the authors namewere written asMulicaMarkus, Horch Raymund, Arkudas Andreas, Cai Aijia, Müller-SeubertWibke, Hauck Theresa and Ludolph Ingo The correct name order is (first name and surname). Markus Mulica, Raymund E. Horch, Andreas Arkudas, Aijia Cai, Wibke Müller- Seubert, Theresa Hauck, Ingo Ludolph Error in Figure Legend In the published article, there was an error in the legend for Figure 5 as published.},
author = {Mulica, Markus and Horch, Raymund E. and Arkudas, Andreas and Cai, Aijia and Müller-Seubert, Wibke and Hauck, Theresa and Ludolph, Ingo},
doi = {10.3389/fsurg.2022.1101481},
faupublication = {yes},
journal = {Frontiers in Surgery},
keywords = {free vascularized bone graft; indocyanine green angiography; medial femoral condyle bone graft; scaphoid non-union; union rate},
month = {Jan},
note = {CRIS-Team Scopus Importer:2023-02-24},
peerreviewed = {Yes},
title = {{Corrigendum}: {Does} indocyanine green fluorescence angiography impact the intraoperative choice of procedure in free vascularized medial femoral condyle grafting for scaphoid non-unions?({Front}. {Surg}., (2022), 9, (962450), 10.3389/fsurg.2022.962450)},
volume = {9},
year = {2023}
}
@article{faucris.124118764,
abstract = {The aim of this prospective study was to assess the correlation of flap perfusion analysis based on laser-assisted Indocyanine Green (ICG) angiography with combined laser Doppler spectrophotometry in autologous breast reconstruction using free DIEP/ms-TRAM flaps. Between February 2014 and July 2015, 35 free DIEP/ms-TRAM flaps were included in this study. Besides the clinical evaluation of flaps, intraoperative perfusion dynamics were assessed by means of laser-assisted ICG angiography and post-capillary oxygen saturation and relative haemoglobin content (rHb) using combined laser Doppler spectrophotometry. Correlation of the aforementioned parameters was analysed, as well as the impact on flap design and postoperative complications. Flap survival rate was 100%. There were no partial flap losses. In three cases, flap design was based on the angiography, contrary to clinical evaluation and spectrophotometry. The final decision on the inclusion of flap areas was based on the angiographic perfusion pattern. Angiography and spectrophotometry showed a correlation in most of the cases regarding tissue perfusion, post-capillary oxygen saturation and relative haemoglobin content. Laser-assisted ICG angiography is a useful tool for intraoperative evaluation of flap perfusion in autologous breast reconstruction with DIEP/ms-TRAM flaps, especially in decision making in cases where flap perfusion is not clearly assessable by clinical signs and exact determination of well-perfused flap margins is difficult to obtain. It provides an objective real-time analysis of flap perfusion, with high sensitivity for the detection of poorly perfused flap areas. Concerning the topographical mapping of well-perfused flap areas, laser-assisted angiography is superior to combined laser Doppler spectrophotometry.},
author = {Ludolph, Ingo and Arkudas, Andreas and Schmitz, Marweh and Boos, Anja and Taeger, Christian and Rother, Ulrich and Horch, Raymund E. and Beier, Justus},
doi = {10.1016/j.bjps.2016.07.014},
faupublication = {yes},
journal = {Journal of Plastic Reconstructive and Aesthetic Surgery},
note = {EVALuna2:15280},
pages = {1382-8},
peerreviewed = {Yes},
title = {{Cracking} the perfusion code?: {Laser}-assisted {Indocyanine} {Green} angiography and combined laser {Doppler} spectrophotometry for intraoperative evaluation of tissue perfusion in autologous breast reconstruction with {DIEP} or ms-{TRAM} flaps},
volume = {69},
year = {2016}
}
@article{faucris.204503309,
abstract = {Introduction:
Autologous fat transfer has recently become an increasingly popular surgical procedure and comprises harvesting, processing and transplantation of adipose tissue, as well as professional follow-up care. This method, as a surgical procedure, can be utilised for trauma-, disease- or age-related soft tissue volume deficits and soft tissue augmentation. As usage is increasing, but the variables of fat harvest, specific indications and fashion of fat transfer are poorly defined, there is a great demand for development of a guideline in the field of reconstructive and aesthetic surgery.Methods:All relevant points were discussed within the scope of a consensus conference including a nominal group process of all societies involved in the procedure and ratified with a strong consensus (>95%). Literature from the standard medical databases over the last 10 years was retrieved, studied and specific guidelines were concluded.Results:Consensus was achieved among all professionals involved on the following points: 1. definition 2. indication/contraindication, 3. preoperative measures 4. donor sites 5. techniques of processing 6. transplantation 7. follow-up care 8. storage 9. efficacy 10. documentation 11. evaluation of patient safety.Conclusion:Definite indications and professional expertise are paramount for autologous fat tissue transfer. Successful transfers are based on the use of correct methods as well as specific instruments and materials. Autologous adipose tissue transplantation is considered to be a safe procedure in reconstructive and aesthetic surgery, due to the low rate of postoperative complications and sequelae.},
author = {Prantl, L. and Rennekampff, H. O. and Giunta, R. E. and Harder, Y. and Von Heimburg, D. and Heine, N. and Herold, C. and Kneser, U. and Lampert, F. and Machens, H. G. and Mirastschijski, Ursula and Mueller, D. and Pallua, N. and Schantz, T. and Schnborn, A. and Ueberreiter, K. and Witzel, Ch. and Bull, G. and Rezek, D. and Sattler, G. and Vogt, P. M. and Horch, Raymund E.},
doi = {10.1055/s-0042-117635},
faupublication = {yes},
journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie},
note = {EVALuna2:32822},
pages = {330-336},
peerreviewed = {Yes},
title = {{Current} {Perceptions} of {Lipofilling} on the {Basis} of the {New} {Guideline} on "{Autologous} {Fat} {Grafting}"},
volume = {48},
year = {2016}
}
@inproceedings{faucris.106676724,
author = {Schubert, Dirk W. and Lämmlein, Michael and Horch, Raymund E.},
booktitle = {Book of Abstracts},
date = {2016-05-09/2016-05-13},
faupublication = {yes},
pages = {159-160},
peerreviewed = {unknown},
title = {{Cyclic} {Loading} {Of} {Silicone} {Breast} {Implatns} {Influence} {On} {The} {Failure} {Statistics} - {A} {Model} {Study}},
venue = {Posen},
year = {2016}
}
@article{faucris.106740524,
abstract = {Introduction Patients with low rectal cancer or anal cancer undergoing abdominoperineal excision (APE) benefit from extended surgery and the subsequent avoidance of surgical "waisting" at the level of the puborectalis muscle. The method of cylindrical APE was introduced by T. Holm and led to a reduction of intraoperative perforations and involvement of circumferential resection margins, and subsequently reduced the risk of local recurrence. The use of myocutaneous flaps reduces perineal wound complications, which occur in up to 60% of patients with primary closure of perineal defects, especially following neoadjuvant radiochemotherapy. Flaps obliterate pelvic dead space, recruit well-vascularised tissue into irradiated regions, facilitate wound closure and allow for vaginal and perineal reconstructions. This video shows the technique of extended cylindrical APE with partial vulvar and vaginal resection and subsequent reconstruction of the posterior vaginal wall and the pelvic floor defect by a vertical rectus abdominis myocutaneous (VRAM) flap. Indication Locally advanced anal cancer with infiltration and fistula to the posterior vaginal wall without metastatic spread following neoadjuvant radiochemotherapy. Procedure Extended cylindric APE with partial vulvar and vaginal resection, construction of a descending colostomy with parastomal intraperitoneal onlay mesh augmentation, pelvic reconstruction with a VRAM flap and inlay mesh augmentation of the anterior rectus sheath. Conclusion From the oncological point of view, extralevator APE is superior to standard surgery. The use of myocutaneous flaps improves postoperative wound healing and quality of life.},
author = {Krautz, Christian and Weber, Klaus and Croner, Roland and Denz, Axel and Maak, Matthias and Horch, Raymund E. and Grützmann, Robert},
doi = {10.1055/s-0043-117172},
faupublication = {yes},
journal = {Zentralblatt für Chirurgie},
note = {EVALuna2:15291},
pages = {543-547},
peerreviewed = {Yes},
title = {{Cylindric} {Abdominoperineal} {Rectum} {Exstirpation} with {Partial} {Vulvar} and {Vaginal} {Resection} as well as {Perineal} and {Vaginal} {Defect} {Reconstruction} by a {Vertical} {Rectus} {Abdominis} {Myocutaneous} ({VRAM}) {Flap}},
volume = {142},
year = {2017}
}
@article{faucris.259313149,
abstract = {Zusammenfassung Der tiefe sternale Wundinfekt (TSWI) ist eine seltene Komplikation nach Sternotomie, die mit hoher Morbiditat und Mortalitat behaftet ist und Krankenhausaufenthalt sowie die Behandlungskosten signifikant ansteigen lasst. Eine mikrobiologische Besonderheit ist die Ausbildung von Biofilmen auf Implantatmaterial und/oder Knochensequestern. Die Diagnose wird anhand der klinischen Symptomatik der lokalen Entzundungsreaktion gestellt und durch systemische Infektionszeichen gestutzt. Eine fruhzeitige und gemeinsame Konsensfindung, welche die oft interdisziplinare Behandlungsstrategie festlegt, ist dabei essenziell. Die Behandlung besteht i.d.R.aus mehreren chirurgischen Wunddebridements mit zwischenzeitlicher Wundkonditionierung, bis ein ausreichend sauberer Wundstatus erreicht ist. Zur Infektsanierung und fur den Wundverschluss stehen je nach Gro ss e und Lokalisation des Gewebedefektes eine Vielzahl verschiedener gestielter und freier Lappenplastiken zur Auswahl.},
author = {Harder, Yves and Krapf, Johanna and Rieck, Bernd and Schaefer, Dirk J. and Djedovic, Gabriel and Kutschka, Ingo and Graf, Karolin and Kneser, Ulrich and Horch, Raymund E. and Lehnhardt, Marcus and Pierer, Gerhard},
doi = {10.1055/a-1425-5987},
faupublication = {yes},
journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie},
note = {CRIS-Team WoS Importer:2021-05-28},
peerreviewed = {Yes},
title = {{Deep} sternal wound infection after sternotomy {Report} of the consensus workshop of the {German}-{Speaking} {Society} for {Microsurgery} of {Peripheral} {Nerves} and {Vessels} ({DAM})},
year = {2021}
}
@article{faucris.107268744,
author = {Horch, Raymund E. and Schultz, Gregory and Schubert, Dirk W.},
faupublication = {yes},
journal = {Plastische Chirurgie},
pages = {233 - 240},
peerreviewed = {Yes},
title = {{Der} bakterielle {Biofilm} auf {Brustprothesen} - eine aktuelle {Übersicht}},
volume = {12},
year = {2012}
}
@article{faucris.220853060,
abstract = {Introduction: Dermatofibrosarcoma protuberans (DFSP) is a rare, low-grade malignant mesenchymal tumor of the soft tissue, characterized by slow infiltrative growth and common local recurrence, with rare distant metastases. Patients and methods: We present a retrospective study of nineteen patients who were diagnosed with DFSP and operated at our institution in > 10-year period. We examined the clinicopathological parameters with special emphasis on the margin status regarding the clinical outcome and the follow-up. Results: A total of eight cases underwent re-excision at our institution following primary excision or incisional biopsy performed at a different institution. Seven cases received excision after incisional biopsy at our institution. Four patients developed recurrent disease following primary excision with histological R0 margins at other institutions and received re-excision at our institution. All excisions at our institution resulted in R0 margins with no recurrence recorded at last follow-up (6 to 175; mean 84 months). The mean margin for those who received resection at our institution was 1.67 cm. Negative margins upon primary excision were achieved using a mean margin width of 2.04 cm. Most common tumor localization was the trunk (10 cases). Conclusion: Awareness of this rare entity is important for a prompt diagnosis and a proper management of the disease. The greatest clinical challenge in the management of DFSP is achieving local control. Complete excision of the tumor with surgical margin widths of at least 2 cm is recommended.},
author = {Wiesmüller, Felix and Agaimy, Abbas and Perrakis, Aristotelis and Arkudas, Andreas and Horch, Raymund E. and Grützmann, Robert and Vassos, Nikolaos},
doi = {10.1186/s12957-019-1627-3},
faupublication = {yes},
journal = {World Journal of Surgical Oncology},
keywords = {Cutaneous lesions; Dermatofibrosarcoma protuberans; DFSP; Mesenchymal tumor; Wallace rule of 9s},
note = {CRIS-Team Scopus Importer:2019-06-18},
peerreviewed = {Yes},
title = {{Dermatofibrosarcoma} protuberans: {Surgical} management of a challenging mesenchymal tumor},
volume = {17},
year = {2019}
}
@article{faucris.229367896,
abstract = {Reconstructive microsurgery using free and pedicled flaps has become a reliable method with a high success rate. Preoperative perforator mapping and intraoperative assessment of perfusion might further reduce flap-associated morbidity.There are various techniques for perforator mapping and perfusion measurement, but no guidelines regarding their use. Therefore, an expert panel at the 40th Annual Meeting of the German-Speaking Working Group for Microsurgery of the Peripheral Nerves and Vessels (DAM) discussed and critically reviewed the current literature. The consensus statement represents the expert opinion based on the available literature and provides recommendations regarding the use of preoperative perforator mapping and intraoperative perfusion measurement.},
author = {Schmauss, D. and Schmauss, D. and Beier, J. P. and Eisenhardt, S. U. and Horch, Raymund E. and Momeni, A. and Rab, M. and Rieck, B. and Rieger, U. and Schaefer, D. J. and Schmidt, V. J. and Kneser, U.},
doi = {10.1055/a-0987-0118},
faupublication = {yes},
journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie},
note = {CRIS-Team Scopus Importer:2019-11-19},
pages = {410-417},
peerreviewed = {Yes},
title = {{Der} sichere {Lappen} – {Präoperatives} {Gefäß}-{Mapping} und intraoperative {Perfusionsmessung} zur {Reduktion} der lappenbedingten {Morbidität}},
volume = {51},
year = {2019}
}
@article{faucris.110518144,
abstract = {Volumetric analysis of the kidney parenchyma provides additional information for the detection and monitoring of various renal diseases. Therefore the purposes of the study were to develop and evaluate a semi-automated segmentation tool and a modified ellipsoid formula for volumetric analysis of the kidney in non-contrast T2-weighted magnetic resonance (MR)-images. Three readers performed semi-automated segmentation of the total kidney volume (TKV) in axial, non-contrast-enhanced T2-weighted MR-images of 24 healthy volunteers (48 kidneys) twice. A semi-automated threshold-based segmentation tool was developed to segment the kidney parenchyma. Furthermore, the three readers measured renal dimensions (length, width, depth) and applied different formulas to calculate the TKV. Manual segmentation served as a reference volume. Volumes of the different methods were compared and time required was recorded. There was no significant difference between the semi-automatically and manually segmented TKV (p = 0.31). The difference in mean volumes was 0.3 ml (95% confidence interval (CI), -10.1 to 10.7 ml). Semi-automated segmentation was significantly faster than manual segmentation, with a mean difference = 188 s (220 vs. 408 s); p < 0.05. Volumes did not differ significantly comparing the results of different readers. Calculation of TKV with a modified ellipsoid formula (ellipsoid volume × 0.85) did not differ significantly from the reference volume; however, the mean error was three times higher (difference of mean volumes -0.1 ml; CI -31.1 to 30.9 ml; p = 0.95). Applying the modified ellipsoid formula was the fastest way to get an estimation of the renal volume (41 s). Semi-automated segmentation and volumetric analysis of the kidney in native T2-weighted MR data delivers accurate and reproducible results and was significantly faster than manual segmentation. Applying a modified ellipsoid formula quickly provides an accurate kidney volume.},
author = {Seuß, Hannes and Janka, Rolf Matthias and Pruemmer, Marcus and Hammon, Rebecca and Theis, Ragnar and Sandmair, Martin and Amann, Kerstin Ute and Bäuerle, Tobias and Uder, Michael and Hammon, Matthias and Cavallaro, Alexander Josef},
doi = {10.1007/s10278-016-9936-3},
faupublication = {yes},
journal = {Journal of Digital Imaging},
note = {EVALuna2:14622},
pages = {244-254},
peerreviewed = {Yes},
title = {{Development} and {Evaluation} of a {Semi}-automated {Segmentation} {Tool} and a {Modified} {Ellipsoid} {Formula} for {Volumetric} {Analysis} of the {Kidney} in {Non}-contrast {T2}-{Weighted} {MR} {Images}},
volume = {30},
year = {2017}
}
@article{faucris.204506008,
abstract = {Background
Breast cancer is the world's most common cancer among women. Autologous lipotransfer is increasingly used for breast reconstruction following surgical removal of the tumour. In cell-assisted lipotransfer, the transplant is enriched with stem cells from adipose tissue (ADSC). Despite positive clinical results, there are some concerns regarding oncological safety due to transplanted stem cells. To date there are only a few breast cancer studies using primary cells from the same patient to enable further investigation into the complexity of cell-cell interactions in breast cancer in an experimental setting.Materials and methodsWe performed literature research on the topic of autologous lipotransfer. 5 different cell types (epithelial, mesenchymal cells, ADSC, endothelial cells, endothelial progenitor cells) were isolated from mammary (carcinoma) tissue or blood and were subsequently characterised for gene and protein expression as well as functional properties. The arteriovenous (AV) loop model in the rat was evaluated as a possible in vivo model for breast cancer pathogenesis and angiogenesis in this study.ResultsThe literature provided evidence for an in-vitro interaction between ADSC and cells of the mammary (carcinoma) tissue. In some clinical studies, certain subgroups of patients appeared to be exposed to an increased risk of tumour recurrence after lipotransfer, but in most studies no correlation between lipotransfer and tumour recurrence was found. Different cell populations, which differed significantly in terms of surface markers, gene expression and functional properties, were isolated from tissue of the same patient. Axial vascularised tissue was successfully generated in the AV loop model.ConclusionIn this study we were able to isolate different cell populations from the same patient, which reflect the heterogeneity of the tumour tissue. This enables a precise analysis of cell-cell interactions and their effects on tumour angiogenesis and pathogenesis in breast cancer. In combination with the AV loop model, this offers new possibilities to generate vascularised mammary carcinoma tissue as well as healthy mammary gland tissue in vivo as an optimal model for the clinical setting.},
author = {Weigand, Annika and Tasbihi, Kereshmeh and Strissel, Pamela and Strick, Reiner and Horch, Raymund E. and Boos, Anja},
doi = {10.1055/s-0042-123706},
faupublication = {yes},
journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie},
note = {EVALuna2:32854},
pages = {111-122},
peerreviewed = {Yes},
title = {{Development} of an {Innovative} {Cell} {Isolation} {Method} for the {Investigation} of {Breast} {Cancer} {Pathogenesis} and {Angiogenesis} for {Experimental} {In} {Vitro} {And} {In} {Vivo} {Assays}},
volume = {49},
year = {2017}
}
@article{faucris.242199247,
abstract = {BACKGROUND: To manage the expected COVID-19 patient load major restrictions in in- and outpatient treatment had to be made. Depending on local conditions and order supply differences SARS-CoV-2 restrictions had a massive impact on medical care. To show the impact of plastic surgery on emergency surgery during SARS-CoV-2 pandemic, the amount of surgical emergencies in a single center plastic surgery division were evaluated. METHOD: The number of plastic surgery cases in a university hospital was evaluated during 16.03.2020 to 27.04.2020 and compared with previous years. RESULTS: Due to cancelling of elective surgery the number of cases dropped to 57,3 % of the caseload of previous years. There was no change in ratio of emergency (2020: 56,4 %; 2017-2019: 54,9 %) and urgent (2020: 44,6 %; 2017-2019: 45 %) surgery. No changes in regard to the etiology of trauma cause nor insurance status (occupational insurance/health insurance) were noted. CONCLUSION: Based on the data of this evaluation there is a clear relevance of Plastic Surgery in the setting of general medical care. Even during the pandemic crises a sufficient plastic surgery service is mandatory in a tertiary referral center.},
author = {Bernuth, Silvia and Horch, Raymund E. and Vater, Adrian and Fuchs, Konrad and Jakubietz, Michael G. and Schmidt, Karsten and Meffert, Rainer H. and Jakubietz, Rafael G.},
doi = {10.1055/a-1220-6920},
faupublication = {yes},
journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie},
note = {CRIS-Team Scopus Importer:2020-09-04},
pages = {272-279},
peerreviewed = {Yes},
title = {{Die} {Auswirkungen} der {SARS}-{CoV}-2-{Einschränkungen} auf die {Patientenversorgung} im {Fachbereich} für {Plastische}, {Rekonstruktive} und {Ästhetische} {Chirurgie}},
volume = {52},
year = {2020}
}
@inproceedings{faucris.113251204,
author = {Schubert, Dirk W. and Horch, Raymund E. and Kaschta, Joachim},
booktitle = {Book of Abstracts},
date = {2013-06-16/2013-06-21},
faupublication = {yes},
peerreviewed = {unknown},
title = {{Differentiating} gel compositions of silicone breast implants from {Poly} {Implant} {Prothèse} and other {Manufactures}},
venue = {Pisa},
year = {2013}
}
@article{faucris.231299645,
abstract = {Objective: Expression of dipeptidylpeptidase 4 (DPP-4) identifies a dermal fibroblast lineage involved in scarring during wound healing. The role of DDP-4 in tissue fibrosis is, however, unknown. The aim of the present study was to evaluate DPP-4 as a potential target for the treatment of fibrosis in patients with systemic sclerosis (SSc). Methods: Expression of DPP-4 in skin biopsy samples and dermal fibroblasts was analyzed by real-time polymerase chain reaction, immunofluorescence, and Western blot analyses. The activity of DPP-4 was modulated by overexpression, knockdown, and pharmacologic inhibition of DPP4 using sitagliptin and vildagliptin. The effects of DPP4 inhibition were analyzed in human dermal fibroblasts and in different mouse models of SSc (each n = 6). Results: The expression of DPP-4 and the number of DPP-4–positive fibroblasts were increased in the fibrotic skin of SSc patients, in a transforming growth factor β (TGFβ)–dependent manner. DPP-4–positive fibroblasts expressed higher levels of myofibroblast markers and collagen (each P < 0.001 versus healthy controls). Overexpression of DPP4 promoted fibroblast activation, whereas pharmacologic inhibition or genetic inactivation of DPP4 reduced the proliferation, migration, and expression of contractile proteins and release of collagen (each P < 0.001 versus control mice) by interfering with TGFβ-induced ERK signaling. DPP4-knockout mice were less sensitive to bleomycin-induced dermal and pulmonary fibrosis (P < 0.0001 versus wild-type controls). Treatment with DPP4 inhibitors promoted regression of fibrosis in mice that had received bleomycin challenge and mice with chronic graft-versus-host disease, and ameliorated fibrosis in TSK1 mice (each P < 0.001 versus untreated controls). These antifibrotic effects were associated with a reduction in inflammation. Conclusion: DPP-4 characterizes a population of activated fibroblasts and shows that DPP-4 regulates TGFβ-induced fibroblast activation in the fibrotic skin of SSc patients. Inhibition of DPP4 exerts potent antifibrotic effects when administered in well-tolerated doses. As DPP4 inhibitors are already in clinical use for diabetes, these results may have direct translational implications for the treatment of fibrosis in patients with SSc.},
author = {Soare, Alina and Györfi, Andrea-Hermina and Matei, Alexandru-Emil and Dees, Clara and Rauber, Simon and Wohlfahrt, Thomas and Chen, Chih Wei and Ludolph, Ingo and Horch, Raymund E. and Bäuerle, Tobias and von Hörsten, Stephan and Mihai, Carina and Distler, Oliver and Schett, Georg and Distler, Jörg and Ramming, Andreas},
doi = {10.1002/art.41058},
faupublication = {yes},
journal = {Arthritis and Rheumatology},
month = {Jan},
note = {CRIS-Team Scopus Importer:2020-01-10},
pages = {137-149},
peerreviewed = {Yes},
title = {{Dipeptidylpeptidase} 4 as a {Marker} of {Activated} {Fibroblasts} and a {Potential} {Target} for the {Treatment} of {Fibrosis} in {Systemic} {Sclerosis}},
volume = {72},
year = {2020}
}
@article{faucris.227596858,
author = {Kengelbach-Weigand, Annika and Horch, Raymund E.},
doi = {10.1097/PRS.0000000000006047},
faupublication = {yes},
journal = {Plastic and Reconstructive Surgery},
note = {CRIS-Team Scopus Importer:2019-10-08},
pages = {881-882},
peerreviewed = {Yes},
title = {{Discussion}: {Extracellular} {Vesicles} from {Human} {Adipose}-{Derived} {Stem} {Cells} for the {Improvement} of {Angiogenesis} and {Fat}-{Grafting} {Application}},
volume = {144},
year = {2019}
}
@article{faucris.204504005,
abstract = {Background
Fractures or fracture dislocations of the proximal interphalangeal joint often occur during sports or accidents. Dislocations of the PIP-joint are the most common ligamentary injuries of the hand. As this kind of injury is so frequent, hand surgeons and other physicians should be aware of the correct treatment.Objectives This paper summarises the most common injury patterns and the correct treatment of PIP-joint dislocations.Materials and Methods This paper reviews the current literature and describes the standardised treatment of PIP-joint dislocations.Results What is most important is that reposition is anatomically correct, and this should be controlled by X-ray examination. Depending on the instability and possible combination with other injuries (e. g. injury to the palmar plate), early functional physiotherapy of the joint or a short immobilisation period is indicated.Conclusions Early functional treatment of the injured PIP-joint, initially using buddy taping, is important to restore PIP-joint movement and function. Depending on the injury, joint immobilisation using a K-wire may be indicated. Detailed informed consent is necessary to explain to the patient the severity of the injury and possible complications, such as chronic functional disorders or development of arthrosis.},
author = {Müller-Seubert, Wibke and Bührer, Gregor and Horch, Raymund E.},
doi = {10.1055/s-0043-115191},
faupublication = {yes},
journal = {Sportverletzung-Sportschaden},
note = {EVALuna2:32840},
pages = {154-159},
peerreviewed = {Yes},
title = {{Dislocation} of the {PIP}-{Joint} - {Treatment} of a common (ball)sports injury},
volume = {31},
year = {2017}
}
@article{faucris.123215884,
author = {Horch, Raymund E. and Lang, Werner and Meyer, Alexander and Schmitz, Marweh},
doi = {10.1111/iwj.12282},
faupublication = {yes},
journal = {International Wound Journal},
note = {EVALuna2:15173},
peerreviewed = {Yes},
title = {{Distal} pedal bypasses combined with free microsurgical flaps in chronic limb ischaemia for problematic wounds},
year = {2014}
}
@article{faucris.282412692,
abstract = {Background: Free vascularized medial femoral condyle (MFC) bone grafts can lead to increased vascularity of the proximal pole and restore scaphoid architecture in scaphoid nonunions. The intraoperative perfusion assessment of the bone graft is challenging because the conventional clinical examination is difficult. Indocyanine green (ICG) angiography has previously been shown to provide a real-time intraoperative evaluation of soft tissue perfusion in reconstructive surgery. The present study investigated the utility of ICG angiography in patients treated with a free medial femoral condyle graft for scaphoid nonunions. Methods: We performed a retrospective analysis of patients with scaphoid nonunions, in which ICG angiography was used intraoperatively for perfusion assessment. The medical records, radiographs, intraoperative imaging, and operative reports of all patients were reviewed. Intraoperative ICG dye was administered intravenously, and laser angiography was performed to assess bone perfusion. The scaphoid union was examined using postoperative CT scans. Results: Two patients had documented osteonecrosis of the proximal pole at the time of surgery. Four patients received a nonvascularized prior bone graft procedure, and a prior spongiosa graft procedure was performed in one patient. The mean time from injury to the MFC bone graft surgery was 52.7 months, and the mean time from prior failed surgery was 10.4 months. Perfusion of the vascular pedicle of the MFC and the periosteum could be detected in all patients. In two patients, even perfusion of the cancellous bone could be demonstrated by ICG angiography. Following transplantation of the bone graft, patency of the vascular anastomosis and perfusion of the periost were confirmed by ICG angiography in the assessed cases. No additional surgery regarding a salvage procedure for a scaphoid nonunion advanced collapse was necessary for the further course. Conclusion: ICG-angiography has shown to be a promising tool in the treatment of scaphoid nonunion with medial femoral condyle bone grafts. It enables intraoperative decision making by assessment of the microvascular blood supply of the periosteum and the vascular pedicle of the MFC bone graft. Further studies need to evaluate the impact on union rates in a long-term follow-up.},
author = {Mulica, Markus and Horch, Raymund E. and Andreas, Arkudas and Cai, Aijia and Müller-Seubert, Wibke and Hauck, Theresa and Ingo, Ludolph},
doi = {10.3389/fsurg.2022.962450},
faupublication = {yes},
journal = {Frontiers in Surgery},
keywords = {free vascularized bone graft; indocyanine green angiography; medial femoral condyle bone graft; scaphoid nonunion; union rate},
note = {CRIS-Team Scopus Importer:2022-09-30},
peerreviewed = {Yes},
title = {{Does} indocyanine green fluorescence angiography impact the intraoperative choice of procedure in free vascularized medial femoral condyle grafting for scaphoid nonunions?},
volume = {9},
year = {2022}
}
@article{faucris.243609890,
abstract = {Background: Management of donor site closure after harvesting a vertical rectus abdominis myocutaneous (VRAM) flap is discussed heterogeneously in the literature. We aim to analyze the postoperative complications of the donor site depending on the closure technique. Methods: During a 12-year period (2003–2015), 192 patients in our department received transpelvic VRAM flap reconstruction. Prospectively collected data were analyzed retrospectively. Results: 182 patients received a VRAM flap reconstruction for malignant, 10 patients for benign disease. The median age of patients was 62 years. 117 patients (61%) received a reconstruction of donor site by Vypro® mesh, 46 patients (24%) by Vicryl® mesh, 23 patients (12%) by direct closure and 6 patients (3%) by combination of different meshes. 32 patients (17%) developed in total 34 postoperative complications at the donor site. 22 complications (11%) were treated conservatively, 12 (6%) surgically. 17 patients (9%) developed incisional hernia during follow-up, with highest incidence in the Vicryl® group (n = 8; 17%) and lowest in the Vypro® group (n = 7; 6%). Postoperative parastomal hernias were found in 30 patients (16%) including three patients with simultaneous hernia around an urostomy and a colostomy. The highest incidence of parastomal hernia was found in patients receiving primary closure of the donor site (n = 6; 26%), the lowest incidence in the Vypro® group (n = 16; 14%). Conclusion: The use of Vypro® mesh for donor site closure appears to be associated with a low postoperative incidence of complications and can therefore be recommended as a preferred technique.},
author = {Schellerer, Vera and Bartholomé, Lenka and Langheinrich, Melanie and Grützmann, Robert and Horch, Raymund E. and Merkel, Susanne and Weber, Klaus},
doi = {10.1007/s00268-020-05788-5},
faupublication = {yes},
journal = {World Journal of Surgery},
note = {CRIS-Team Scopus Importer:2020-10-09},
peerreviewed = {Yes},
title = {{Donor} {Site} {Morbidity} of {Patients} {Receiving} {Vertical} {Rectus} {Abdominis} {Myocutaneous} {Flap} for {Perineal}, {Vaginal} or {Inguinal} {Reconstruction}},
year = {2020}
}
@article{faucris.112647964,
abstract = {Sufficient blood supply is a crucial factor determining postoperative allograft function in kidney transplantation. Therefore, besides the surgeon's individual impression a method for evaluating the quality of the organ's microperfusion is required. Laser fluorescence angiography with indocyanine-green (ICG) is an emerging tool for this purpose. However, no reproducible quantification of ICG fluorescence has been performed in transplantation so far.This retrospective two-center study was designed to evaluate the dosing of ICG for intraoperative laser fluorescence angiography in kidney transplantation. The Spy Elite(®) system (NOVADAQ, Canada) was employed for quantitative assessment of allograft microperfusion. ICG was administered systemically 5 min after reperfusion applying doses between 0.25 and 0.01 mg ICG per kg body weight. Quantitative assessment was performed with the implemented SPY-Q Software.57 kidney recipients were included in two centers. The generated curves showing ICG ingress and egress rates were not evaluable due to oversensing when doses exceeded 0.02 mg per kg body weight.Fluorescence angiography with ICG is an emerging tool for the intraoperative quality control and evaluation of microperfusion in kidney transplantation. A dose of 0.02 mg ICG per kg body weight is recommended in order to ensure the quantitative assessment with SPY-Q. This article is protected by copyright. All rights reserved.},
author = {Rother, Ulrich and Gerken, Andreas L. H. and Karampinis, Ioannis and Klumpp, Madeline and Regus, Susanne and Meyer, Alexander and Apel, Hendrik and Kraemer, Bernhard K. and Hilgers, Karl Friedrich and Lang, Werner and Nowak, Kai},
doi = {10.1111/micc.12392},
faupublication = {yes},
journal = {Microcirculation},
note = {EVALuna2:3964},
peerreviewed = {Yes},
title = {{Dosing} of indocyanine-green for intraoperative laser fluorescence angiography in kidney transplantation},
year = {2017}
}
@article{faucris.123238764,
author = {Beier, Justus and Bogdan, Christian and Schmitz, Markus and Schubert, Dirk W. and Schultz, Gregory and Horch, Raymund E.},
faupublication = {yes},
journal = {Chirurgische Allgemeine - Zeitung für Klinik und Praxis},
pages = {467-470},
peerreviewed = {Yes},
title = {{Durch} {Biofilm} bakteriell besiedelte {Implantate} bedingen {Wundheilungsstörungen}},
volume = {14},
year = {2013}
}
@article{faucris.291583889,
author = {Horch, Raymund E. and Kesting, Marco Rainer and Kersting, S. and Fichtner-Feigl, S. and Arkudas, Andreas},
doi = {10.3389/fonc.2023.1146719},
faupublication = {yes},
journal = {Frontiers in Oncology},
keywords = {cancer surgery; interdisciplinary surgical approaches; outcome; quality of life; surgical tumor reconstruction},
note = {CRIS-Team Scopus Importer:2023-03-10},
peerreviewed = {Yes},
title = {{Editorial}: {Interdisciplinary} surgical strategies for complex tumor defects in modern oncology},
volume = {13},
year = {2023}
}
@article{faucris.281713094,
abstract = {The effects of topical negative pressure therapy (TNP) have been a subject of research for many years. In this study, we investigated new imaging devices to detect clinical changes that TNP causes on healthy tissue and identified differences in microcirculation created by different pressure levels. We used near-infrared spectroscopy (NIS), thermography, and a vein illuminator to measure the differences in oxygen saturation, tissue temperature, and vein pattern. A control group (-125 mmHg) and three comparison groups with only TNP dressing (Group 1), -25 mmHg (Group 2), and -175 mmHg (Group 3) were established. Thirty minutes of TNP on intact skin was followed by 30 min of resting. A total of 24 participants were measured by all imaging devices at predetermined time points. Oxygen saturation and skin temperature increased by 8.07% and 1.67 degrees C for the control group, 4.00% and 1.65 degrees C for Group 2, and 8.45% and 1.68 degrees C for Group 3. Group 1 showed a slight increase in oxygen saturation and a 2.7 degrees C increase in skin temperature. Over the 30 min following removal of TNP, oxygen saturation and temperature decreased gradually for all groups. The vein illuminator did not show significant differences in the venous pattern or flow. Our study showed that higher negative pressure values resulted in higher oxygen saturation and higher tissue temperature.},
author = {Aslan-Horch, Emine Ceylan and Horch, Raymund E. and Arkudas, Andreas and Müller-Seubert, Wibke and Ludolph, Ingo},
doi = {10.3390/jcm11175133},
faupublication = {yes},
journal = {Journal of Clinical Medicine},
note = {CRIS-Team WoS Importer:2022-09-16},
peerreviewed = {Yes},
title = {{Effects} of {Different} {Pressure} {Levels} in {Topical} {Negative} {Pressure} {Application}-{Analysis} of {Perfusion} {Parameters} in a {Clinical} {Skin} {Model} {Using} {Multimodal} {Imaging} {Techniques}},
volume = {11},
year = {2022}
}
@article{faucris.227891222,
abstract = {BACKGROUND: Topical negative pressure wound therapy (TNPWT) is one of the most frequently used techniques in wound treatment. But some of the underlying mechanisms still remain unclear. One possible explanation is an improved microcirculation by TNPWT.},
author = {Muenchow, S. and Horch, Raymund E. and Dragu, Adrian},
doi = {10.3233/CH-180536},
faupublication = {yes},
journal = {Clinical Hemorheology and Microcirculation},
month = {Jan},
note = {CRIS-Team WoS Importer:2019-10-15},
pages = {365-374},
peerreviewed = {Yes},
title = {{Effects} of topical negative pressure therapy on perfusion and microcirculation of human skin},
volume = {72},
year = {2019}
}
@inproceedings{faucris.118117824,
author = {Schubert, Dirk W. and Kaschta, Joachim and Horch, Raymund E. and Walter, Bastian and Daenicke, Jonas and Wichert, Franziska},
booktitle = {Würzbuger Tage 2014},
date = {2014-04-21/2014-04-22},
faupublication = {yes},
peerreviewed = {unknown},
title = {{Eine} {Geschichte} des {Versagens} - der {PIP} {Skandal}},
venue = {Würzburg},
year = {2014}
}
@inproceedings{faucris.121928224,
author = {Schubert, Dirk W. and Kaschta, Joachim and Horch, Raymund E. and Walter, Bastian and Daenicke, Jonas and Wichert, Franziska},
booktitle = {Würzburger Tage 2014},
date = {2014-03-20/2014-03-21},
faupublication = {yes},
pages = {185-192},
peerreviewed = {unknown},
title = {{Eine} {Geschichte} des {Versagens} - der {PIP} {Skandal}},
venue = {Würzburg},
year = {2014}
}
@article{faucris.238258305,
abstract = {Background: Popliteal artery aneurysms (PAAs) are the most common peripheral aneurysms. Although rare and often asymptomatic, there is a significant risk of thrombosis, embolism, and limb loss. The aim of this study was to evaluate the eligibility for endovascular repair of patients treated for symptomatic and asymptomatic PAAs in accordance with the instructions for use (IFU). Methods: All patients treated for PAA with open surgical repair between the years 2010 and 2017 were analyzed if suitable for endovascular treatment. Preoperative imaging was reviewed for applicability with an interventional radiologist and 2 vascular surgeons. Evaluation was performed in accordance with the following criteria adopted from the IFU of the Gore ® Viabahn stent graft: at least a single-vessel tibial runoff, proximal and distal landing zone more than 2 cm, no large difference in vessel diameter proximal and distal to the aneurysm, no overstenting of significant collaterals necessary, and no inadequate kinking of the artery. The patients were classified in 3 categories: the patient was eligible, endovascular treatment was feasible, and endovascular treatment was not appropriate. Results: 51 patients with 61 symptomatic and asymptomatic PAAs were identified. Forty-five cases were asymptomatic, 11 cases showed clinical symptoms such as claudication, and in 5 cases, the patients presented with acute ischemia. Twenty-four patients were eligible for endovascular intervention, 14 cases were feasible, and in 23 cases, it was not appropriate in accordance with the IFU. Conclusions: In this study, more than one-third of the patients with PAA were not eligible for endovascular treatment in accordance with the IFU and another 23 % showed substantial reasons against endovascular treatment. These data suggest that endovascular repair remains a treatment option for selected patients only. Cross-sectional imaging is mandatory for procedure selection.},
author = {Hellwig, Konstantin and Hoffmann, Lisa and Rother, Ulrich and Meyer, Alexander and Lang, Werner and Schmid, Axel},
doi = {10.1016/j.avsg.2020.03.006},
faupublication = {yes},
journal = {Annals of Vascular Surgery},
note = {CRIS-Team Scopus Importer:2020-05-12},
peerreviewed = {Yes},
title = {{Eligibility} of {Endovascular} {Repair} for {Popliteal} {Artery} {Aneurysms} {According} the {Instructions} for {Use}},
year = {2020}
}
@article{faucris.109624504,
abstract = {Although being a safe and standardised procedure, free-flap reconstruction can be harmful if unpredictable situations occur intraoperatively. The case presented reveals a situation in which an unscheduled interdisciplinary approach allowed to complete our reconstructive aim. An extensive defect at the thigh was planned for reconstruction by means of a free rectus abdominis flap. As the distant part of the flap showed a compromised perfusion during operation and had to be partially discarded, our colleagues from the vascular surgery department created an arterio-venous loop for anastomosis. This allowed a more distant positioning of the flap and ensured a complete defect reconstruction.},
author = {Taeger, Christian and Arkudas, Andreas and Beier, Justus and Horch, Raymund E.},
doi = {10.1111/iwj.12278},
faupublication = {yes},
journal = {International Wound Journal},
note = {EVALuna2:15569},
pages = {598-600},
peerreviewed = {Yes},
title = {{Emergency} arterio-venous loop for free-flap defect reconstruction of the lower thigh with a post-irradiated and heavily infected wound},
volume = {12},
year = {2015}
}
@article{faucris.210313387,
abstract = {DiI-labeled MSCs/mL ADA-GEL. Four weeks postoperatively, tissue formation and vascularization were investigated by histology and microcomputed tomography. We were able to prove vascularization originating from the AV loop in both groups with statistically significant more vessels in group B containing MSCs. Moreover, encapsulated MSCs promoted biodegradation of the ADA-GEL microcapsules. In the present study, we were able to demonstrate for the first time, the successful vascularization of ADA-GEL microcapsules by means of the AV loop. Furthermore, ADA-GEL displayed a good biocompatibility and encapsulation of MSCs into ADA-GEL microcapsule-enhanced vascularization as well as biodegradation.},
author = {Steiner, Dominik and Lingens, Lara and Fischer, Laura and Köhn, Katrin and Detsch, Rainer and Boccaccini, Aldo R. and Fey, Tobias and Greil, Peter and Weis, Christian and Beier, Justus and Horch, Raymund E. and Arkudas, Andreas},
doi = {10.1089/ten.TEA.2017.0496},
faupublication = {yes},
journal = {Tissue Engineering: Parts A, B, and C},
pages = {1320-1331},
peerreviewed = {unknown},
title = {{Encapsulation} of {Mesenchymal} {Stem} {Cells} {Improves} {Vascularization} of {Alginate}-{Based} {Scaffolds}.},
volume = {24},
year = {2018}
}
@article{faucris.210313656,
abstract = {Alginate dialdehyde (ADA), gelatin, and nano-scaled bioactive glass (nBG) particles are being currently investigated for their potential use as three-dimensional scaffolding materials for bone tissue engineering. ADA and gelatin provide a three-dimensional scaffold with properties supporting cell adhesion and proliferation. Combined with nanocristalline BG, this composition closely mimics the mineral phase of bone. In the present study, rat bone marrow derived mesenchymal stem cells (MSCs), commonly used as an osteogenic cell source, were evaluated after encapsulation into ADA-gelatin hydrogel with and without nBG. High cell survival was found in vitro for up to 28 days with or without addition of nBG assessed by calcein staining, proving the cell-friendly encapsulation process. After subcutaneous implantation into rats, survival was assessed by DAPI/TUNEL fluorescence staining. Hematoxylin-eosin staining and immunohistochemical staining for the macrophage marker ED1 (CD68) and the endothelial cell marker lectin were used to evaluate immune reaction and vascularization. After in vivo implantation, high cell survival was found after 1 week, with a notable decrease after 4 weeks. Immune reaction was very mild, proving the biocompatibility of the material. Angiogenesis in implanted constructs was significantly improved by cell encapsulation, compared to cell-free beads, as the implanted MSCs were able to attract endothelial cells. Constructs with nBG showed higher numbers of vital MSCs and lectin positive endothelial cells, thus showing a higher degree of angiogenesis, although this difference was not significant. These results support the use of ADA/gelatin/nBG as a scaffold and of MSCs as a source of osteogenic cells for bone tissue engineering. Future studies should however improve long term cell survival and focus on differentiation potential of encapsulated cells in vivo.},
author = {Rottensteiner-Brandl, Ulrike and Detsch, Rainer and Sarker, Bapi and Lingens, Lara and Köhn, Katrin and Kneser, Ulrich and Boßerhoff, Anja Katrin and Horch, Raymund E. and Boccaccini, Aldo R. and Arkudas, Andreas},
doi = {10.3390/ma11101880},
faupublication = {yes},
journal = {Materials},
peerreviewed = {Yes},
title = {{Encapsulation} of {Rat} {Bone} {Marrow} {Derived} {Mesenchymal} {Stem} {Cells} in {Alginate} {Dialdehyde}/{Gelatin} {Microbeads} with and without {Nanoscaled} {Bioactive} {Glass} for {In} {Vivo} {Bone} {Tissue} {Engineering}.},
volume = {11},
year = {2018}
}
@article{faucris.221415052,
abstract = {Carpal tunnel syndrome is a very common condition in hand surgery. The gold standard in therapy is the surgical release of the flexor retinaculum. Endoscopic carpal tunnel release provides superior convalescence and patient safety. In this video paper, we demonstrate endoscopic carpal tunnel release via a monoportal approach and report our experience in treating over 800 cases.},
author = {Hessenauer, Maximilian Emanuel Thomas and Horch, Raymund E.},
doi = {10.1055/a-0832-2560},
faupublication = {yes},
journal = {Zentralblatt für Chirurgie},
keywords = {carpal tunnel; endoscopic; monoportal},
note = {CRIS-Team Scopus Importer:2019-06-28},
pages = {219-221},
peerreviewed = {Yes},
title = {{Endoscopic} {Carpal} {Tunnel} {Release} {Via} a {Monoportal} {Approach} {Die} endoskopische {Karpaltunnelspaltung} im monoportalen {Verfahren}},
volume = {144},
year = {2019}
}
@article{faucris.229227494,
abstract = {Vascularization of bioartificial matrices is crucial for successful tissue engineering. Endothelial progenitor cells (EPC) have shown vascularization potential in ischemic conditions and may also support blood vessel formation in tissue-engineered matrices. The aim of our study was to investigate the impact of a well-characterized murine embryonal EPC line (T17b-EPC) on vascularization and fibrovascular granulation tissue formation after suspension in a fibrine matrix followed by subcutaneous implantation in a separation chamber in rats. EPC were fluorescently labelled in vitro prior to implantation. After 3, 7 or 14 days, animals were killed followed by explantation and histological analysis of the constructs. Before the end of the experiment, Bandeirea Simplicifolia lectin was intravenously injected to mark the vascular ingrowth into the implanted constructs. The transplanted cells were histologically detected at all time-points and located almost exclusively within the fibrin matrix at day 3 but the number of cells in the clot continuously decreased over day 7 to day 14. Conversely, cells were detected within the newly formed granulation tissue in increasing numbers from day 3 over day 7 to day 14. Transplanted cells were also found in the intermuscular septa. Cell viability was confirmed by use of an EPC clone expressing β-galactosidase. Fluorescence microscopy demonstrated integration of the transplanted cells in newly formed blood vessels within the fibrovascular granulation tissue adjacent to the fibrin clot. Presence of cells in the fibrin clot lead to thicker granulation tissue and an increased blood vessel diameter compared to cell-free controls. Organ standard controls showed presence of the transplanted cells in spleens at day 14 after transplantation. In summary, EPC exhibited biological activity after subcutaneous implantation in a fibrin matrix by migration from the fibrin clot into the granulation tissue and along intermuscular septae, undergoing differentiation into mature endothelial cells and integration into newly formed blood vessels and altering fibrovascular granulation tissue development. EPC may hold promise to modulate blood vessel formation in bioartificial matrices. © 2011 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.},
author = {Bleiziffer, Oliver and Hammon, Matthias and Naschberger, Elisabeth and Lipnik, Karoline and Arkudas, Andreas and Rath, Subha and Pryymachuk, Galyna and Beier, Justus and Stürzl, Michael and Horch, Raymund E. and Kneser, Ulrich},
doi = {10.1111/j.1582-4934.2010.01247.x},
faupublication = {yes},
journal = {Journal of Cellular and Molecular Medicine},
keywords = {Angiogenesis; Endothelial progenitor cells; Fibrin matrix; Tissue engineering},
note = {Created from Fastlane, Scopus look-up},
pages = {2452-2461},
peerreviewed = {Yes},
title = {{Endothelial} progenitor cells are integrated in newly formed capillaries and alter adjacent fibrovascular tissue after subcutaneous implantation in a fibrin matrix},
volume = {15},
year = {2011}
}
@article{faucris.215443456,
abstract = {The mechanical behaviour of gel‐filled silicone breast implants is
examined under compressive load. First the implants are tested by
applying various loads in a repetitive fashion until shell rupture
occurs. Subsequently the results are presented allowing an assessment of
the correlation between the number of loading cycles and the maximum
compressive load. Finally it is possible to estimate the endurance of
the silicone breast implants. Using a power law, a correlation between
endurance and maximum load is revealed},
author = {von Hanstein, Harald and Horch, Raymund E. and Schubert, Dirk W.},
doi = {10.1002/pi.5760},
faupublication = {yes},
journal = {Polymer International},
keywords = {Silicone breast implant; mechanical behavior; compression test; mechanical loading; endurance},
note = {CRIS-Team WoS Importer:2019-04-04},
pages = {648-650},
peerreviewed = {Yes},
title = {{Endurance} of gel-filled silicone breast implants as a function of maximum load in cyclic compression tests},
volume = {68},
year = {2019}
}
@article{faucris.238463113,
abstract = {This study demonstrates for the first time successful vascularization of solid porous matrices by means of an AV loop. Injection of osteogenic cells into axially prevascularized matrices may eventually create functional bioartificial bone tissues for reconstruction of large defects.\nVascularization remains an obstacle to engineering of larger volume bone tissues. Our aim was to induce axial vascularization in a processed bovine cancellous bone (PBCB) matrix using an arteriovenous (AV) loop (artery, vein graft, and vein).\nCustom-made PBCB discs (9 x 5 mm) were implanted into rats. In group A (n = 19), the matrices were inserted into microsurgically constructed AV loops between the femoral vessels using a vein graft from the contralateral side. In group B (n = 19), there was no vascular carrier. The matrices were encased in isolation chambers. After 2, 4, and 8 weeks, the animals were perfused with India ink via the abdominal aorta. Matrices were explanted and subjected to histological and morphometric analysis. Results were compared with intravital dynamic micro & magnetic resonance imaging and scanning electron microscopy images of vascular corrosion replicas.\nIn group A, significant vascularization of the matrix had occurred by the 8th week. At this time, vascular remodeling with organization into vessels of different sizes was evident. Blood vessels originated from all 3 zones of the AV loop. Group A was significantly superior to group B in terms of vascular density and vascularization kinetics.\nDISCUSSION\nINTRODUCTION\nMETHODS\nRESULTS},
author = {Kneser, Ulrich and Polykandriotis, Elias and Ohnolz, Jan and Heidner, Kristina and Grabinger, Lucia and Euler, Simon and Amann, Kerstin Ute and Heß, Andreas and Brune, Kay and Greil, Peter and Stürzl, Michael and Horch, Raymund E.},
doi = {10.1089/ten.2006.12.1721},
faupublication = {yes},
journal = {Tissue Engineering -Larchmont},
pages = {1721-31},
peerreviewed = {Yes},
title = {{Engineering} of vascularized transplantable bone tissues: induction of axial vascularization in an osteoconductive matrix using an arteriovenous loop.},
volume = {12},
year = {2006}
}
@inproceedings{faucris.264587740,
address = {LONDON},
author = {Györfi, Andrea-Hermina and Matei, Alexandru-Emil and Fuchs, Maximilian and Rigau, Aleix and Hong, Xuezhi and Honglin, Z. and Luber, M. and Bergmann, Christina and Dees, Clara and Ludolph, Ingo and Horch, Raymund E. and Distler, O. and Schett, Georg and Kunz, Meik and Distler, Jörg},
booktitle = {ANNALS OF THE RHEUMATIC DISEASES},
doi = {10.1136/annrheumdis-2021-eular.1428},
faupublication = {yes},
note = {CRIS-Team WoS Importer:2021-10-01},
pages = {391-391},
peerreviewed = {unknown},
publisher = {BMJ PUBLISHING GROUP},
title = {{ENGRAILED} 1 {COORDINATES} {CYTOSKELETAL} {ORGANIZATION} {TO} {PROMOTE} {MYOFIBROBLAST} {DIFFERENTIATION} {AND} {FIBROTIC} {TISSUE} {REMODELING}},
year = {2021}
}
@article{faucris.262162544,
abstract = {Transforming growth factor-β (TGFβ) is a key mediator of fibroblast activation in fibrotic diseases, including systemic sclerosis. Here we show that Engrailed 1 (EN1) is reexpressed in multiple fibroblast subpopulations in the skin of SSc patients. We characterize EN1 as a molecular amplifier of TGFβ signaling in myofibroblast differentiation: TGFβ induces EN1 expression in a SMAD3-dependent manner, and in turn, EN1 mediates the profibrotic effects of TGFβ. RNA sequencing demonstrates that EN1 induces a profibrotic gene expression profile functionally related to cytoskeleton organization and ROCK activation. EN1 regulates gene expression by modulating the activity of SP1 and other SP transcription factors, as confirmed by ChIP-seq experiments for EN1 and SP1. Functional experiments confirm the coordinating role of EN1 on ROCK activity and the reorganization of cytoskeleton during myofibroblast differentiation, in both standard fibroblast culture systems and in vitro skin models. Consistently, mice with fibroblast-specific knockout of En1 demonstrate impaired fibroblast-to-myofibroblast transition and are partially protected from experimental skin fibrosis.},
author = {Györfi, Andrea-Hermina and Matei, Alexandru-Emil and Fuchs, Maximilian and Liang, Chunguang and Rigau, Aleix and Hong, Xuezhi and Zhu, Honglin and Luber, Markus and Bergmann, Christina and Dees, Clara and Ludolph, Ingo and Horch, Raymund E. and Distler, Oliver and Wang, Jiucun and Bengsch, Bertram and Schett, Georg and Kunz, Meik and Distler, Jörg},
doi = {10.1084/jem.20201916},
faupublication = {yes},
journal = {Journal of Experimental Medicine},
note = {CRIS-Team Scopus Importer:2021-07-30},
peerreviewed = {Yes},
title = {{Engrailed} 1 coordinates cytoskeletal reorganization to induce myofibroblast differentiation},
volume = {218},
year = {2021}
}
@article{faucris.262432398,
abstract = {Due to its low immunogenic potential and the possibility to fine-tune their properties, materials made of recombinant engineered spider silks are promising candidates for tissue engineering applications. However, vascularization of silk-based scaffolds is one critical step for the generation of bioartificial tissues and consequently for clinical application. To circumvent insufficient vascularization, the surgically induced angiogenesis by means of arteriovenous loops (AVL) represents a highly effective methodology. Here, previously established hydrogels consisting of nano-fibrillary recombinant eADF4(C16) were transferred into Teflon isolation chambers and vascularized in the rat AVL model over 4 weeks. To improve vascularization, also RGD-tagged eADF4(C16) hydrogels were implanted in the AVL model over 2 and 4 weeks. Thereafter, the specimen were explanted and analyzed using histology and microcomputed tomography. We were able to confirm biocompatibility and tissue formation over time. Functionalizing eADF4(C16) with RGD-motifs improved hydrogel stability and enhanced vascularization even outperforming other hydrogels, such as fibrin. This study demonstrates that the scaffold ultrastructure as well as biofunctionalization with RGD-motifs are powerful tools to optimize silk-based biomaterials for tissue engineering applications.},
author = {Steiner, Dominik and Winkler, Sophie and Heltmann-Meyer, Stefanie and Trossmann, Vanessa T. and Fey, Tobias and Scheibel, Thomas and Horch, Raymund E. and Arkudas, Andreas},
doi = {10.1088/1758-5090/ac0d9b},
faupublication = {yes},
journal = {Biofabrication},
note = {CRIS-Team WoS Importer:2021-08-06},
peerreviewed = {Yes},
title = {{Enhanced} vascularization and de novo tissue formation in hydrogels made of engineered {RGD}-tagged spider silk proteins in the arteriovenous loop model},
volume = {13},
year = {2021}
}
@article{faucris.107271384,
abstract = {Reconstruction of large and complex bone segments is a challenging problem facing maxillofacial surgery. The majority of current regenerative approaches rely on extrinsic vascularization, which is deficient after cancer ablation and irradiation. The aim of the study was to investigate the efficacy of intrinsic axial vascularization of synthetic bone scaffolds in the management of critical-size mandibular defects.Scaffold-guided mandibular regeneration in two groups of adult male goats was compared. Only the scaffolds of the second group were axially vascularized via in situ embedding of an arteriovenous loop through microsurgical anastomosis of facial vessels. After 6 months of follow up, both groups were compared through radiological, biomechanical, histological and histomorphometric analysis.The axially vascularized constructs have showed significantly more central vascularization (p = 0.021) and markedly enhanced central bone formation (p = 0.08). The biomechanical characteristics were enhanced, but the difference between both groups was not statistically significant (p = 0.98).Axially vascularized synthetic mandibular grafts show better vascularization at their central regions, permitting more efficient bone regeneration.The encouraging results of the proposed technique could be of benefit in optimizing the reconstruction of large critical-size bone defects.},
author = {Eweida, Ahmad Mahmoud and Nabawi, Ayman Sameh and Abouarab, Mohamed and Kayed, Mohamed and Elhammady, Habashi and Etaby, Ashraf and Khalil, Mohamed Rafik and Shawky, Michael Samir and Kneser, Ulrich and Horch, Raymund E. and Nagy, Naglaa and Marei, Mona Kamal},
doi = {10.1007/s00784-013-1143-8},
faupublication = {yes},
journal = {Clinical Oral Investigations},
note = {EVALuna2:15576},
pages = {1671-8},
peerreviewed = {Yes},
title = {{Enhancing} mandibular bone regeneration and perfusion via axial vascularization of scaffolds},
volume = {18},
year = {2014}
}
@article{faucris.222409722,
abstract = {Intraoperative assessing and postoperative monitoring of the viability of free flaps is of high relevance in reconstructive microsurgery. Today different methods for the evaluation of tissue perfusion are known. Indocyanine Green angiography is an emerging technique among plastic surgeons with a broad scope of applications especially in microsurgical free flap transfer. We demonstrate the value and clinical application of this technique based on representative selected cases where Indocyanine Green angiography was used in microsurgical free flap transfers from different anatomic donor sites during the operation. Hereby perforator selection, flap tailoring, changes of blood flow and patency of anastomoses was judged and decision making was based on the angiographic findings. This method has proven to be valid, reproducible and easy to use. The application is not limited to the evaluation of skin perfusion, but is also applicable to muscle tissue or chimeric or composite flaps. Reliable judgement is especially given for the extent of arterially perfused tissue following complete flap dissection. Moreover, this real-time angiography revealed a high sensitivity for the detection of poorly perfused flap areas, thus supporting the conventional clinical judgement and reducing complications. In summary Indocyanine Green angiography has the potential to reduce flap related complications and to contribute to enhancing and extending the possibilities of free flap surgery.},
author = {Ludolph, Ingo and Horch, Raymund E. and Arkudas, Andreas and Schmitz, Marweh},
doi = {10.3389/fsurg.2019.00039},
faupublication = {yes},
journal = {Frontiers in Surgery},
note = {CRIS-Team WoS Importer:2019-07-16},
peerreviewed = {Yes},
title = {{Enhancing} {Safety} in {Reconstructive} {Microsurgery} {Using} {Intraoperative} {Indocyanine} {Green} {Angiography}},
volume = {6},
year = {2019}
}
@article{faucris.237478617,
author = {Fritschen, Uwe von and Grill, Barbara and Wagner, Juri and Schuster, Horst and Sukhova, Inesa and Giunta, Riccardo E. and Heitmann, Christoph and Andree, Christoph and Horch, Raymund E. and Kneser, Ulrich and Germann, Günter},
doi = {10.1055/a-1089-2836},
faupublication = {yes},
journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie},
note = {CRIS-Team Scopus Importer:2020-04-17},
pages = {E1-},
peerreviewed = {Yes},
title = {{Erratum}: {Qualitätssicherung} in der {Brustrekonstruktion} – {Etablierung} eines prospektiven nationalen {Onlineregisters} für mikrochirurgische {Brustrekonstruktionen}},
volume = {52},
year = {2020}
}
@article{faucris.216842961,
author = {Horch, Raymund E. and Braumann, Chris and Dissemond, Joachim and Lehner, Burkhard and Hirche, Christoph and Woeste, Guido and Wozniak, Gernold and Wetzel-Roth, Walter and Willy, Christian},
doi = {10.1055/a-0881-4595},
faupublication = {yes},
journal = {Zentralblatt für Chirurgie},
note = {CRIS-Team Scopus Importer:2019-05-02},
pages = {152-},
peerreviewed = {Yes},
title = {{Erratum}: {Use} of negative pressure wound therapy with instillation and dwell time for wound {Treatment} - {Results} of an expert consensus conference [{Erratum}: {Einsatz} der {Vakuuminstillationstherapie} für die {Wundbehandlung} - {Ergebnis} einer {Expertenkonsensuskonferenz}]},
volume = {144},
year = {2019}
}
@article{faucris.108610744,
abstract = {Very few microsurgical courses have been offered for medical students in Germany to date. To raise early interest in this technique, which is essential for plastic and reconstructive surgery, and to guide eligible medical students to choose plastic surgery as their specialist field, the Department of Plastic and Hand Surgery, supported by the Faculty of Medicine of the Friedrich-Alexander-University of Erlangen-Nuremberg, implemented a microsurgical course for students in 2011. This study describes the implementation of that course and evaluates its impact on the subsequent choice of the participants' specialist fields.Since the summer of 2011, the microsurgery course for medical students has taken place regularly 3 times per term. It is free of charge for participants and is guided by senior physicians of the Department of Plastic and Hand Surgery together with student tutors from the Faculty of Medicine. The arterial end-to-end anastomosis in the fresh chicken leg is used as a training model. Based on a questionnaire survey the participants were evaluated and statistically analysed regarding their course satisfaction, self-assessment of their own eligibility before and after the course, the anticipated future choice of their medical specialist field and how their choice was influenced by this course.After the successful implementation of the microsurgical course in 2011, a significant number of students were interested in microsurgery. According to the questionnaire, the level of enthusiasm was high among all participants. The self-assessment of microsurgical skills improved significantly after the course compared with the pre-course assessment. In 82% of the participants, the course had a strong positive influence on the future choice of their specialist field.The regular implementation of a microsurgical course for students in the form described here is practicable and possible without undue personnel and cost of materials. The ongoing interest among students in such an offer is enormous and the satisfaction of the participants is very high. This might be a way to recruit future plastic surgeons by raising early enthusiasm for microsurgery. These future plastic surgeons, in turn, would be given the chance to experience a very fascinating aspect of plastic surgery, which might help them to decide on their specialisation within that field at a later point in their career.},
author = {Beier, Justus and Horch, Raymund E. and Boos, Anja and Taeger, Christian and Breuer, Georg and Arkudas, Andreas},
doi = {10.1055/s-0041-108196},
faupublication = {yes},
journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie},
note = {EVALuna2:15603},
pages = {400-7},
peerreviewed = {Yes},
title = {{Establishment} and {Evaluation} of a {Microsurgery} {Course} for {Medical} {Students}},
volume = {47},
year = {2015}
}
@article{faucris.237478115,
abstract = {BACKGROUND: The formation of professional networks and cooperations - in addition to any qualified good education - seems fundamental for a successful career. In a number of disciplines, various symposia or conferences exist. In the field of microsurgery, however, a specific, guided and designated opportunity for junior scientists to network with one another has been missing so far. METHODS: In 2017, a science academy was initiated for the first time by the German-speaking Association for Nerves and Vessels (DAM) with the goal of bringing together and networking microsurgically researching young physicians and scientists. This was intended to happen on a small scale once a year in order to develop synergies for joint research projects. For this purpose, motivated junior researchers were individually selected by their mentors and sent to the academy by the boards of research institutions that are organized in the DAM. After getting to know each other in a relaxed atmosphere, the participants were given the opportunity to present their respective research project within the framework of thematic blocks and moderated by experienced mentors. Each presentation was followed by a round table discussion and small group work, in which knowledge and methods were exchanged and points of contact for possible later cooperation were identified. RESULTS: In the past 3 years, the DAM Science Academy proved to be an optimal format to initiate and promote networks of young researchers comprising microsurgically interested physicians and scientists. There were many lively and in-depth discussions, which were mainly due to the open working atmosphere and the obligation to confidentiality. Most of the synergies were shown i. a. in the field of angiogenesis, bioreactor, carcinoma-ADSC interactions, stem cells, AV loop model, ischemia/reperfusion, and nerve regeneration. The participants consistently gave a very positive feedback in the final evaluation with the wish to continue this academy. CONCLUSION: The DAM Science Academy can be considered a highly suitable complemental platform to the existing networking opportunities among microsurgical researchers. Experience so far suggests that this will hopefully result in long-term cooperations and a permanent transfer of knowledge among the participants.},
author = {Horch, Raymund E. and Kengelbach-Weigand, Annika and Pierer, G. and Kneser, U. and Schaefer, D. and Boos, Anja and Arkudas, Andreas and Schmidt, V. J.},
doi = {10.1055/a-1076-0933},
faupublication = {yes},
journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie},
note = {CRIS-Team Scopus Importer:2020-04-17},
pages = {140-146},
peerreviewed = {Yes},
title = {{Etablierung} und {Evaluation} einer multinationalen {Wissenschaftsakademie}: {Ein} neues {Modell} zur {Stärkung} des wissenschaftlichen {Nachwuchses} in der mikrochirurgischen {Forschung} – {Ein} {Konsensus} {Papier} der {DAM}},
volume = {52},
year = {2020}
}
@article{faucris.111533004,
abstract = {In this study, the angiogenetic effect of sintered 45S5 Bioglass (R) was quantitatively assessed for the first time in the arteriovenous loop (AVL) model. An AVL was created by interposition of a venous graft from the contralateral side between the femoral artery and vein in the medial thigh of eight rats. The loop was placed in a Teflon isolation chamber and was embedded in a sintered 45S5 Bioglass (R) granula matrix filled with fibrin gel. Specimens were investigated 3 weeks postoperatively by means of microcomputed tomography, histological, and morphometrical techniques. All animals tolerated the operations well. At 3 weeks, both microcomputed tomography and histology demonstrated a dense network of newly formed vessels originating from the AVL. All constructs were filled with cell-rich, highly vascularized connective tissue around the vascular axis. Analysis of vessel diameter revealed constant small vessel diameters, indicating immature new vessel sprouts. This study shows for the first time axial vascularization of a sintered 45S5 Bioglass (R) granula matrix. After 3 weeks, the newly generated vascular network already interfused most parts of the scaffolds and showed signs of immaturity. The intrinsic type of vascularization allows transplantation of the entire construct using the AVL pedicle.},
author = {Arkudas, Andreas and Balzer, Amelie and Bührer, Gregor and Arnold, Isabel and Hoppe, Alexander and Detsch, Rainer and Newby, Phillipa and Fey, Tobias and Greil, Peter and Horch, Raymund E. and Boccaccini, Aldo R. and Kneser, Ulrich},
doi = {10.1089/ten.tec.2012.0572},
faupublication = {yes},
journal = {Tissue Engineering - Part C: Methods},
note = {UnivIS-Import:2015-03-09:Pub.2013.tech.IW.LM.evalua},
pages = {479-486},
peerreviewed = {Yes},
title = {{Evaluation} of angiogenesis of bioactive glass in the arteriovenous loop model},
volume = {19},
year = {2013}
}
@article{faucris.123428624,
abstract = {This study aimed to evaluate the diagnostic performance of using a reformatted single-in-plane image reformation of the rib cage for the detection of rib fractures in computed tomography (CT) examinations, employing different levels of radiological experience.We retrospectively evaluated 10 consecutive patients with and 10 patients without rib fractures, whose CT scans were reformatted to a single-in-plane image reformation of the rib cage. Eight readers (two radiologists, two residents in radiology, and four interns) independently evaluated the images for the presence of rib fractures using a reformatted single-in-plane image and a multi-planar image reformation. The time limit was 30 seconds for each read. A consensus of two radiologist readings was considered as the reference standard. Diagnostic performance (sensitivity, specificity, positive predictive value [PPV], and negative predictive value [NPV]) was assessed and evaluated per rib and per location (anterior, lateral, posterior). To determine the time limit, we prospectively analyzed the average time it took radiologists to assess the rib cage, in a bone window setting, in 50 routine CT examinations. McNemar test was used to compare the diagnostic performances.Single image reformation was successful in all 20 patients. The sensitivity, specificity, PPV, and NPV for the detection of rib fractures using the conventional multi-planar read were 77.5%, 99.2%, 89.9%, and 98.0% for radiologists; 46.3%, 99.7%, 92.5%, and 95.3% for residents; and 29.4%, 99.4%, 82.5%, and 93.9% for interns, respectively. Sensitivity, PPV, and NPV increased across all three groups of experience, using the reformatted single-in-plane image of the rib cage (radiologists: 85.0%, 98.6%, and 98.7%; residents: 80.0%, 92.8%, and 98.2%; interns: 66.9%, 89.9%, and 97.1%), whereas specificity did not change significantly (99.9%, 99.4%, and 99.3%). The diagnostic performance of the interns and residents was significantly better when evaluating the single-in-plane image reformations (P < .01). The diagnostic performance of the radiologists was better when evaluating the single-in-plane image reformations; however, there was no significant difference (statistical power: 0.32).The diagnostic performance for the detection of rib fractures, using CT images that have been reformatted to a single-in-plane image, improves for readers from different educational levels when the evaluation time is restricted to 30 seconds or less.},
author = {Dankerl, Peter and Seuß, Hannes and Ellmann, Stephan and Uder, Michael and Hammon, Matthias and Cavallaro, Alexander Josef},
doi = {10.1016/j.acra.2016.09.022},
faupublication = {yes},
journal = {Academic Radiology},
note = {EVALuna2:14593},
peerreviewed = {Yes},
title = {{Evaluation} of {Rib} {Fractures} on a {Single}-in-plane {Image} {Reformation} of the {Rib} {Cage} in {CT} {Examinations}},
year = {2016}
}
@inproceedings{faucris.113916264,
author = {Daenicke, Jonas and Schubert, Dirk W. and Gedde, Ulf and Hedenqvist, Mikael and Linde, Erik and Kaschta, Joachim and Horch, Raymund E.},
booktitle = {Nordic Polymer Days},
date = {2015-06-01/2015-06-03},
faupublication = {yes},
peerreviewed = {unknown},
title = {{Evaluation} of the diffusion properties of cyclic siloxanes through the silicone breast implant shell},
venue = {Kopenhagen},
year = {2015}
}
@inproceedings{faucris.113918464,
author = {Daenicke, Jonas and Schubert, Dirk W. and Gedde, Ulf and Hedenqvist, Mikael and Linde, Erik and Kaschta, Joachim and Horch, Raymund E.},
booktitle = {14th Bayreuth Polymer Symposium},
date = {2015-09-20/2015-09-22},
faupublication = {yes},
peerreviewed = {unknown},
title = {{Evaluation} of the diffusion properties of cyclic siloxanes through the silicone breast implant shell},
venue = {Bayreuth},
year = {2015}
}
@article{faucris.216149254,
abstract = {Driven by the continuing discussion on safety and quality of silicone
breast implants, they have turned into focus of this study with respect
to the diffusivity of low molar mass components from the silicone gel
filling into the silicone breast implant shell. Therefore, the
diffusivity of silicone oils into silicone elastomers were analysed by
means of the crosslink density and the penetrant size. The study was
focused on the diffusion of the cyclic siloxanes
Octamethylcyclotetrasiloxane (D4), Decamethylcyclopentasiloxane (D5) and
Dodecamethyl-cyclohexasiloxane (D6) due to their potential occurrence
in silicone breast implants. The analysis of the diffusion behavior was
carried out with silicone breast implant shells taken from explants and
tailor-made silicone elastomer samples varying in crosslink density.
Therefore, sorption experiments were performed. The subsequent
evaluation of the sorption data yield to the corresponding diffusion
properties. Based on the diffusion coefficient related to the crosslink
density a model was developed to describe the material behavio},
author = {Daenicke, Jonas and Schubert, Dirk W. and Hedenqvist, Mikael and Linde, Erik and Sigl, Thomas and Horch, Raymund E.},
doi = {10.1063/1.5084878},
faupublication = {yes},
journal = {AIP Conference Proceedings},
keywords = {polymer, materials analysis},
month = {Jan},
note = {CRIS-Team WoS Importer:2019-05-03},
peerreviewed = {unknown},
title = {{Evaluation} of the influence of crosslink density and penetrant size on the diffusion properties of silicone oils into silicone elastomers},
url = {https://aip.scitation.org/doi/abs/10.1063/1.5084878},
volume = {2055},
year = {2019}
}
@article{faucris.281717120,
abstract = {Background: The exact influence of tourniquet ischemia on a treated extremity remains unclear. Methods: Twenty patients received an operation on one hand under tourniquet ischemia. Twenty healthy volunteers received 10 min of tourniquet ischemia on one of their arms. Measurements of tissue oxygen saturation using near-infrared reflectance-based imaging and skin temperature of the dorsum of the hand were performed at five different timepoints (t0 was performed just before the application of the tourniquet ischemia, t1 directly after the application of the tourniquet ischemia, t2 before the release of the ischemia, t3 directly after the release of the ischemia, and t4 on the following day). Results: In both groups, tissue oxygen saturation dropped after the application of the tourniquet ischemia compared to t0 and increased after the release of the tourniquet ischemia. In the patient group, tissue oxygen saturation at t4 was higher compared to t0; in contrast, the level of tissue oxygen saturation in the participant group dropped slightly at t4 compared to t0. The measured skin temperature in the patient group showed an increase during the observation period, while it continuously decreased in the group of healthy participants. Conclusions: Short-term ischemia did not appear to permanently restrict perfusion in this study design. The non-invasive imaging modalities used were easy to handle and allowed repetitive measurement.},
author = {Müller-Seubert, Wibke and Herold, Helen and Graf, Stephanie and Ludolph, Ingo and Horch, Raymund E.},
doi = {10.3390/jcm11175240},
faupublication = {yes},
journal = {Journal of Clinical Medicine},
note = {CRIS-Team WoS Importer:2022-09-16},
peerreviewed = {Yes},
title = {{Evaluation} of the {Influence} of {Short} {Tourniquet} {Ischemia} on {Tissue} {Oxygen} {Saturation} and {Skin} {Temperature} {Using} {Two} {Portable} {Imaging} {Modalities}},
volume = {11},
year = {2022}
}
@article{faucris.204502082,
abstract = {Commentary to the paper "What went wrong? Conciliatory proceedings of a German mediation center after breast reductions", Allert S., Flechtner C., Vogt P. M. et al. Handchir Mikrochir Plast Chir, DOI 10.1055/s-0042-103586.
},
author = {Horch, Raymund E.},
doi = {10.1055/s-0042-103587},
faupublication = {yes},
journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie},
note = {EVALuna2:32828},
pages = {108-10},
peerreviewed = {Yes},
title = {{Examination} of {Medical} {Malpractice} {Allegations} by {Arbitration} {Boards}},
volume = {48},
year = {2016}
}
@article{faucris.252993849,
abstract = {Background: Treating advanced finger joint contractures from Dupuytren disease remains a challenge. We evaluated the effectiveness of a skeletal distraction device versus alternative treatment options. Material/Methods: We analyzed the surgical treatment of contracted finger joints in stage III and stage IV Dupuytren’s disease over a 10-year period. Data were obtained from inpatient and outpatient medical records, including postoperative clinical examinations and extended Disabilities of the Arm, Shoulder, and Hand (DASH) questionnaire scores. Complications of infection, postoperative pain, and wound healing disorders were recorded. Results: A total of 79 patients (83 hands) were assigned to 2 treatment groups. Patients in group 1 underwent an initial open transection of the main fibrous cord, Z-plasty, distraction with the Erlangen external distraction device, and fasciectomy. The distraction period was 13 to 81 days (mean 31 days). Group 2 underwent a conventional single-stage fasciectomy and arthrolysis. DASH scores and subjective patient satisfaction were lower in group 1 (20.7/33%) than in group 2 (10.3/50%). However, the staged approach of group 1 to treat proximal interphalangeal joint contractures in the long term (improvement >40%) was more effective than the approach of group 2 (>33%). Distraction device pin infections occurred in 20% of hands. Postoperative pain and complex regional pain syndrome type I occurred in 25% of hands in group 1 and 3% in group 2. Conclusions: A screw thread driven external fixation device is useful in end-stage Dupuytren’s finger joint contractures. It is indicated when joint contractures are advanced and simple arthrolysis is insufficient.},
author = {Horch, Raymund E. and Schmitz, Marweh and Kreuzer, Maria and Arkudas, Andreas and Ludolph, Ingo and Müller-Seubert, Wibke},
doi = {10.12659/MSM.929814},
faupublication = {yes},
journal = {Medical Science Monitor},
keywords = {Dupuytren contracture; External fixators; Joint capsule release},
note = {CRIS-Team Scopus Importer:2021-03-26},
peerreviewed = {Yes},
title = {{External} screw-threaded traction device helps optimize finger joint mobility in severe stage {III} and {IV} dupuytren disease},
volume = {27},
year = {2021}
}
@article{faucris.118142024,
abstract = {In free flap surgery, a clinically established concept still has to be found for the reduction of ischemia-related cell damage in the case of prolonged ischemia. Although promising results using extracorporeal free flap perfusion in the laboratory have been published in the past, until now this concept has not yet paved its way into clinical routine. This might be due to the complexity of perfusion systems and a lack of standardized tools. Here, we want to present the results of the first extracorporeal free flap perfusion in a clinical setting using a simple approach without the application of a complex perfusion machinery.},
author = {Taeger, Christian and Präbst, Konstantin and Beier, Justus and Meyer, A. and Horch, Raymund E.},
doi = {10.1097/GOX.0000000000000672},
faupublication = {yes},
journal = {Plastic and Reconstructive Surgery Global Open},
note = {EVALuna2:15616},
pages = {e682},
peerreviewed = {Yes},
title = {{Extracorporeal} {Free} {Flap} {Perfusion} in {Case} of {Prolonged} {Ischemia} {Time}},
volume = {4},
year = {2016}
}
@article{faucris.221612632,
author = {Taeger, Christian D. and Lamby, Philipp and Dolderer, Jürgen and Philipp, Alois and Kehrer, Andreas and Horch, Raymund E. and Präbst, Konstantin and Prantl, Lukas},
doi = {10.1097/SLA.0000000000003226},
faupublication = {yes},
journal = {Annals of Surgery},
note = {CRIS-Team Scopus Importer:2019-07-02},
pages = {e5-e6},
peerreviewed = {Yes},
title = {{Extracorporeal} {Perfusion} for {Salvage} of {Major} {Amputates}},
volume = {270},
year = {2019}
}
@article{faucris.106686184,
abstract = {BACKGROUND
Extracorporeal perfusion (EP) is moving into focus of research in reconstructive and transplantation medicine for the preservation of amputates and free tissue transplants. The idea behind EP is the reduction of ischemia-related cell damage between separation from blood circulation and reanastomosis of the transplant. Most experimental approaches are based on a complex system that moves the perfusate in a circular course.
OBJECTIVE AND METHODS
In this study, we aimed to evaluate if a simple perfusion by an infusion bag filled with an electrolyte solution can provide acceptable results in terms of flow stability, oxygen supply and viability conservation for EP of a muscle transplant. The results are compared to muscles perfused with a pump system as well as muscles stored under ischemic conditions with a one-time intravasal flushing with Jonosteril.
RESULTS
With this simple method a sufficient oxygen supply could be achieved and functionality could be maintained between 3.35 times and 4.60 times longer compared to the control group. Annexin V positive nuclei, indicating apoptosis, increased by 9.7% in the perfused group compared to 24.4% in the control group.
CONCLUSIONS
Overall, by decreasing the complexity of the system, EP by one-way infusion can become more feasible in clinical situation},
author = {Taeger, Christian Dirk and Friedrich, Oliver and Horch, Raymund E. and Drechsler, Caroline and Weigand, Annika and Hopf, Fabio and Geppert, Carol-Immanuel and Münch, Frank and Birkholz, Torsten and Wenzel, Carina and Geis, Sebastian and Prantl, Lukas and Buchholz, Rainer and Präbst, Konstantin},
doi = {10.3233/CH-170298},
faupublication = {yes},
journal = {Clinical hemorheology and microcirculation},
peerreviewed = {Yes},
title = {{Extracorporeal} perfusion - reduced to a one-way infusion.},
year = {2017}
}
@inproceedings{faucris.123001604,
author = {Schubert, Dirk W. and Horch, Raymund E. and Kaschta, Joachim and Daenicke, Jonas and Walter, Bastian},
booktitle = {13th Bayreuth Polymer Symposium},
date = {2013-09-15/2013-09-17},
faupublication = {yes},
peerreviewed = {unknown},
title = {{Failure} of {Silicone} {Breast} {Implants} – {New} {Insights} by {Mapping} the {Mechanical} {Properties} of {Implant} {Shells}},
venue = {Bayreuth},
year = {2013}
}
@inproceedings{faucris.122413984,
author = {Schubert, Dirk W. and Kaschta, Joachim and Horch, Raymund E. and Walter, Bastian and Daenicke, Jonas},
booktitle = {Book of Abstracts},
date = {2013-06-16/2013-06-21},
faupublication = {yes},
peerreviewed = {unknown},
title = {{Failure} of {Silicone} {Breast} {Implants} – {New} {Insights} by {Mapping} the {Mechanical} {Properties} of {Implant} {Shells}},
venue = {Pisa},
year = {2013}
}
@inproceedings{faucris.113895144,
author = {Schubert, Dirk W. and Horch, Raymund E. and Kaschta, Joachim and Daenicke, Jonas and Walter, Bastian},
booktitle = {Nordic Polymer Days},
date = {2013-05-29/2013-05-31},
faupublication = {yes},
peerreviewed = {unknown},
title = {{Failure} of {Silicone} {Breast} {Implants} – {Novel} {Insights} by {Mapping} the {Mechanical} {Properties} of {Implant} {Shells}},
venue = {Helsinki},
year = {2013}
}
@article{faucris.238463382,
abstract = {The modulation of angiogenic processes in matrices is of great interest in tissue engineering. We assessed the angiogenic effects of fibrin-immobilized VEGF and bFGF in an arteriovenous loop (AVL) model in 22 AVLs created between the femoral artery and vein in rats. The loops were placed in isolation chambers and were embedded in 500 microL fibrin gel (FG) (group A) or in 500 microL FG loaded with 0.1 ng/microL VEGF and 0.1 ng/microL bFGF (group B). After two and four weeks specimens were explanted and investigated using histological, morphometrical, and ultramorphological [scanning electron microscope (SEM) of vascular corrosion replicas] techniques. In both groups, the AVL induced formation of densely vascularized connective tissue with differentiated and functional vessels inside the fibrin matrix. VEGF and bFGF induced significantly higher absolute and relative vascular density and a faster resorption of the fibrin matrix. SEM analysis in both groups revealed characteristics of an immature vascular bed, with a higher vascular density in group B. VEGF and bFGF efficiently stimulated sprouting of blood vessels in the AVL model. The implantation of vascular carriers into given growth factor-loaded matrix volumes may eventually allow efficient generation of axially vascularized, tissue-engineered composites.},
author = {Arkudas, Andreas and Tjiawi, Jimmy and Bleiziffer, Oliver and Grabinger, Lucia and Polykandriotis, Elias and Beier, Justus P. and Stürzl, Michael and Horch, Raymund E. and Kneser, Ulrich},
doi = {10.2119/2007-00057.Arkudas},
faupublication = {yes},
journal = {Molecular Medicine},
keywords = {Vascular Endothelial Growth Factor (VEGF); Fibrin Matrix; Arteriovenous Loop (AVL); Vascular Carrier; High Vascular Density},
pages = {480-7},
peerreviewed = {Yes},
title = {{Fibrin} gel-immobilized {VEGF} and {bFGF} efficiently stimulate angiogenesis in the {AV} loop model.},
volume = {13},
year = {2007}
}
@article{faucris.212707553,
abstract = {BackgroundThere is a consensus that after a flexor tendon repair an aggressive rehabilitation protocol with early active motion can improve functional outcome, provided that the combination of material and suturing technique can meet the higher biomechanic demands. Bearing this in mind we evaluated a polytetrafluoroethylene (PTFE) suture (SERAMON((R)), Serag-Wiessner) as a possible material for flexor tendon repair.Materials and methods40 flexor tendons were harvested from fresh cadaveric upper extremities. 3-0 and 5-0 strands were used both in the polypropylene (PPL) as well as in the PTFE group. In the first phase of the study, we evaluated knotting properties and mechanical characteristics of the suture materials themselves. In the second phase, a 2-strand Kirchmayr-Kessler suture technique was applied for a core suture of a flexor tendon (n=16). In the third phase, we performed a tendon repair including an epitendinous running suture with 5-0 PPL or 5-0 PTFE material (n=22). One way ANOVA tests were performed.ResultsThe linear loading strength of single strand knotted PPL 3-0 was 19.870.59N. The linear loading strength of knotted PTFE 3-0 was 32.47 +/- 1.67N. For PPL 3-0 maximum linear strength was achieved with five knots, for PTFE 3-0 with eight knots. When a Kirchmayr-Kessler core-only repair was performed, then in the PPL group the loading strength of the repaired tendon was 30.74 +/- 9.77N. In the PTFE group the loading strength was 23.74 +/- 5.6N (p=0.10). However, all repairs in the PTFE group failed due to cheese wiring. When a Kirchmayr-Kessler core and epitendinous repair technique was used, then in the PPL group the loading strength of the repaired tendon was 49.90 +/- 16.05N. In the PTFE group the loading strength was 73.41 +/- 19.81N (p=0.006).Conclusion p id=Par4 PTFE demonstrates superior strength properties in comparison to PPL for flexor tendon repairs. However, standard 2 strand techniques have proved inadequate to bear the higher biomechanic demands.},
author = {Polykandriotis, Elias and Besrour, Foued and Arkudas, Andreas and Ruppe, Florian and Zetzmann, Katharina and Bräuer, Lars and Horch, Raymund E.},
doi = {10.1007/s00402-018-03105-3},
faupublication = {yes},
journal = {Archives of Orthopaedic and Trauma Surgery},
note = {CRIS-Team WoS Importer:2019-03-08},
pages = {429-434},
peerreviewed = {Yes},
title = {{Flexor} tendon repair with a polytetrafluoroethylene ({PTFE}) suture material},
volume = {139},
year = {2019}
}
@article{faucris.122159444,
abstract = {Exposing a vein to altered hemodynamics by creating an arteriovenous (AV) shunt evokes considerable vessel formation that may be of therapeutic potential. However, it is unclear whether the introduction of oscillatory flow and/or flow increase is decisive. To distinguish between these mechanical stimuli we grafted a femoral vein into the arterial flow pathway of the contralateral limb in rats creating an arterioarterial (AA) loop (n = 7). Alternatively, we connected the femoral artery and vein using the vein graft, whereby we created an AV-loop (n = 27). Vessel loops were embedded in a fibrin filled chamber and blood flow was measured by means of flow probes immediately after surgery (day 0) and 15 days after loop creation. On day 15, animals were sacrificed and angiogenesis was evaluated using ?CT and histological analysis. Mean flow increased from 0.5 to 2.4 mL/min and was elevated throughout the cardiac cycle at day 0 in AV-loops whereas, as expected, it remained unchanged in AA-loops. Flow in AV-loops decreased with time, and was at day 15 not different from untreated femoral vessels or AA-loop grafts. Pulsatile flow oscillations were similar in AV-and AA-loops at day 0. The flow amplitude amounted to ~1.3 mL/min which was comparable to values in untreated arteries. Flow amplitude remained constant in AA-loops, whereas it decreased in AV-loops (day 15: 0.4 mL/min). A large number of newly formed vessels were present in AV-loops at day 15 arising from the grafted vein. In marked contrast, angiogenesis originating from the grafted vein was absent in AA-loops. We conclude that exposure to substantially increased flow is required to initiate angiogenesis in grafted veins, whereas selective enhancement of pulsatile flow is unable to do so. This suggests that indeed flow and most likely wall shear stress is decisive to initiate formation of vessels in this hemodynamically driven angiogenesis model.},
author = {Schmidt, Volker J. and Hilgert, Johannes and Covi, Jennifer M. and Leibig, Nico and Wietbrock, Johanna O. and Arkudas, Andreas and Polykandriotis, Elias and De Wit, Cor and Horch, Raymund E. and Kneser, Ulrich},
doi = {10.1371/journal.pone.0117407},
faupublication = {yes},
journal = {PLoS ONE},
note = {EVALuna2:15583},
pages = {e0117407},
peerreviewed = {Yes},
title = {{Flow} increase is decisive to initiate angiogenesis in veins exposed to altered hemodynamics},
volume = {10},
year = {2015}
}
@article{faucris.204723248,
abstract = {BACKGROUND The arteriovenous (AV) loop model enables axial vascularization to gain a functional microcirculatory system in tissue engineering constructs in vivo. These constructs might replace surgical flaps for the treatment of complex wounds in the future. Today, free flaps are often exposed to high-dose radiation after defect coverage, according to guideline-oriented treatment plans. Vascular response of AV loop-based constructs has not been evaluated after radiation, although it is of particular importance. It is further unclear whether the interposed venous AV loop graft is crucial for the induction of angiogenesis. MATERIAL AND METHODS We exposed the grafted vein to a single radiation dose of 2 Gy prior to loop construction to alter intrinsic and angio-inductive properties specifically within the graft. Vessel loops were embedded in a fibrin-filled chamber for 15 days and radiation-induced effects on flow-mediated vascularization were assessed by micro-CT and two-dimensional histological analysis. RESULTS Vessel amount was significantly impaired when an irradiated vein graft was used for AV loop construction. However, vessel growth and differentiation were still present. In contrast to vessel density, which was homogeneously diminished in constructs containing irradiated veins, vessel diameter was primarily decreased in the more peripheral regions. CONCLUSIONS Vascular luminal sprouts were significantly diminished in irradiated venous grafts, suggesting that the interposing vein constitutes a vital part of the AV loop model and is essential to initiate flow-mediate angiogenesis. These results add to the current understanding of AV loop-based neovascularization and suggest clinical implications for patients requiring combined AV loop-based tissue transfer and adjuvant radiotherapy.},
author = {Schmidt, Volker J. and Covi, Jennifer M. and Koepple, Christoph and Hilgert, Johannes and Polykandriotis, Elias and Bigdeli, Amir K. and Distel, Luitpold and Horch, Raymund E. and Kneser, Ulrich},
faupublication = {yes},
journal = {Medical Science Monitor},
note = {EVALuna2:32850},
pages = {834-842},
peerreviewed = {Yes},
title = {{Flow} {Induced} {Microvascular} {Network} {Formation} of {Therapeutic} {Relevant} {Arteriovenous} ({AV}) {Loop}-{Based} {Constructs} in {Response} to {Ionizing} {Radiation}},
volume = {23},
year = {2017}
}
@article{faucris.234050816,
abstract = {INTRODUCTION: Grip strength and load distribution of the hand are important parameters for evaluating hand function. The purpose of this study was to analyze and compare grip force and load distribution of dominant and nondominant hands in right-handed healthy subjects.
METHODS: Gripping measurements were performed on 40 healthy right-handed subjects using a cylindrical gripping device. Two different cylinders with circumferences of 150 mm (small cylinder) and 200 mm (large cylinder), respectively, were used for the measurements. Subjects were assigned to either the small or the large cylinder with respect to their hand size. Maximum and mean force applied during three intervals of gripping as well as the percent contribution of each digit, thenar, and hypothenar in relation to the total load applied were acquired. Values of dominant and nondominant hands were compared.
RESULTS: Percent contribution of mean grip strength differed for the thumb (p = 0.007), ring finger (p < 0.001), little finger (p = 0.047), and palm (p < 0.001). Comparing the dominant and nondominant side, the dominant hand showed a lower contribution of the thumb, ring finger, and little finger, but a higher contribution of the palm. When analyzing maximum grip, percent contribution of the small fingers was equal between dominant and nondominant side (p = 0.1). Differences between dominant and nondominant thumb, ring finger, and palm persisted (p = 0.007, p = 0.001, p = 0.005, respectively). No differences could be shown for the index finger, middle finger, thenar, and hypothenar when analyzing both mean and maximum force.
DISCUSSION AND CONCLUSION: Percent contribution of the thumb and the fingers to total grip strength differed between dominant and nondominant hands with a change in distribution when assessing maximum grip force. In right-handed subjects, thumb and ring finger have important roles during gripping.},
author = {Pingel, Isabel and Lorz, Daniel and Beier, Justus and Horch, Raymund E. and Arkudas, Andreas and Cai, Aijia},
doi = {10.1007/s00402-018-2997-7},
faupublication = {yes},
journal = {Archives of Orthopaedic and Trauma Surgery},
note = {EVALuna2:211471},
pages = {1323-1331},
peerreviewed = {Yes},
title = {{Force} distribution of a cylindrical grip differs between dominant and nondominant hand in healthy subjects},
volume = {138},
year = {2018}
}
@article{faucris.210632997,
abstract = {Although free flap reconstruction has already gained widespread acceptance in pediatric patients, little is known about the outcome of free tissue transfer in head and neck reconstruction in pediatric patients. We present a case of a 6-month-old boy with a large volume deficit in the right temporal fossa after resection of a teratoma. This led to a large volume deficit with widely undermined skin margins. Therefore, we provided volume augmentation by microsurgical free latissimus dorsi myocutaneous flap transplantation. Intraoperative use of laser-assisted indocyanine green angiography indicated excellent flap perfusion. Postoperative magnetic resonance imaging showed adequate flap perfusion with no signs of flap necrosis. To our best knowledge, this case presents the youngest patient who underwent free flap transplantation in the head and neck region. Our case demonstrates that microvascular surgery can play an important role in particular cases in pediatric oncology, even in very young patients.},
author = {Fried, Frederik W. and Beier, Justus and Bohr, Christopher and Iro, Heinrich and Horch, Raymund E. and Arkudas, Andreas},
doi = {10.1097/SAP.0000000000001629},
faupublication = {yes},
journal = {Annals of Plastic Surgery},
note = {EVALuna2:35384},
pages = {62-63},
peerreviewed = {Yes},
title = {{Free} {Latissimus} {Dorsi} {Myocutaneous} {Flap} in a 6-{Month}-{Old} {Child} for {Reconstruction} of a {Temporal} {Fossa} {Defect} {After} {Teratoma} {Resection}},
volume = {82},
year = {2019}
}
@article{faucris.264586744,
abstract = {Healing of large bone defects remains a challenge in reconstructive surgery, especially with impaired healing potential due to severe trauma, infection or irradiation. In vivo studies are often performed in healthy animals, which might not accurately reflect the situation in clinical cases. In the present study, we successfully combined a critical-sized femoral defect model with an ionizing radiation protocol in rats. To support bone healing, tissue-engineered constructs were transferred into the defect after ectopic preossification and prevascularization. The combination of SiHA, MSCs and BMP-2 resulted in the significant ectopic formation of bone tissue, which can easily be transferred by means of our custom-made titanium chamber. Implanted osteogenic MSCs survived in vivo for a total of 18 weeks. The use of SiHA alone did not lead to bone formation after ectopic implantation. Analysis of gene expression showed early osteoblast differentiation and a hypoxic and inflammatory environment in implanted constructs. Irradiation led to impaired bone healing, decreased vascularization and lower short-term survival of implanted cells. We conclude that our model is highly valuable for the investigation of bone healing and tissue engineering in pre-damaged tissue and that healing of bone defects can be substantially supported by combining SiHA, MSCs and BMP-2.},
author = {Rottensteiner-Brandl, Ulrike and Bertram, Ulf and Lingens, Lara and Köhn, Katrin and Distel, Luitpold and Fey, Tobias and Körner, Carolin and Horch, Raymund E. and Arkudas, Andreas},
doi = {10.3390/cells10092256},
faupublication = {yes},
journal = {Cells},
keywords = {bone tissue engineering; critical size defect; irradiation; mesenchymal stem cells; hydroxyapatite},
note = {CRIS-Team WoS Importer:2021-10-01},
peerreviewed = {Yes},
title = {{Free} {Transplantation} of a {Tissue} {Engineered} {Bone} {Graft} into an {Irradiated}, {Critical}-{Size} {Femoral} {Defect} in {Rats}},
volume = {10},
year = {2021}
}
@article{faucris.290417029,
abstract = {Aim: This prospective clinical study aimed to evaluate whether it would be possible to reduce the rate of re-excisions using CMOS technology, a specimen radiography system (SRS) or digital breast tomosynthesis (DBT) compared to a conventional full field digital mammography (FFDM) system. Material and Method: Between 12/2012 and 2/2013 50 patients were diagnosed with invasive breast cancer (BI-RADS™ 5). After histological verification, all patients underwent breast-conserving therapy with intraoperative imaging using 4 different systems and differing magnifications: 1. Inspiration™ (Siemens, Erlangen, Germany), amorphous selenium, tungsten source, focus 0.1 mm, resolution 85 m pixel pitch, 8 lp/mm; 2. BioVision™ (Bioptics, Tucson, AZ, USA), CMOS technology, photodiode array, flat panel, tungsten source, focus 0.05, resolution 50 m pixel pitch, 12 lp/mm; 3. the Trident™ specimen radiography system (SRS) (Hologic, Bedford, MA, USA), amorphous selenium, tungsten source, focus 0.05, resolution 70 m pixel pitch, 7.1 lp/mm; 4. tomosynthesis (Siemens, Erlangen, Germany), amorphous selenium, tungsten source, focus 0.1 mm, resolution 85 m pixel pitch, 8 lp/mm, angular range 50 degrees, 25 projections, scan time > 20 s, geometry: uniform scanning, reconstruction: filtered back projection. The 600 radiographs were prospectively shown to 3 radiologists. Results: Of the 50 patients with histologically proven breast cancer (BI-RADS™ 6), 39 patients required no further surgical therapy (re-excision) after breast-conserving surgery. A retrospective analysis (n = 11) showed a significant (p < 0.05) increase of sensitivity with the BioVision™, the Trident™ and tomosynthesis compared to the Inspiration™ at a magnification of 1.0: 2.0 or 1.0: 1.0 (tomosynthesis) (2.6, 3.3 or 3.6 %), i.e. re-excision would not have been necessary in 2, 3 or 4 patients, respectively, compared to findings obtained with a standard magnification of 1.0: 1.0. Conclusion: The sensitivity of the BioVision™, the Trident™ and tomosynthesis was significantly (p < 0.05) higher and the rate of re-excisions was reduced compared to FFDM using a conventional detector at a magnification of 2.0 but without zooming. © Georg Thieme Verlag KG Stuttgart New York.},
author = {Schulz-Wendtland, Rüdiger and Dilbat, G. and Bani, Mayada and Fasching, Peter and Heusinger, K. and Lux, Michael P. and Löhberg, Christian and Brehm, Barbara and Hammon, Matthias and Saake, Marc and Dankerl, Peter and Jud, Sebastian and Rauh, Claudia and Bayer, Christian M. and Beckmann, Matthias and Uder, Michael and Meier-Meitinger, Martina},
doi = {10.1055/s-0032-1328600},
faupublication = {yes},
journal = {Geburtshilfe und Frauenheilkunde},
keywords = {Breast; breast cancer; breast malignancy; mammography},
note = {CRIS-Team Scopus Importer:2023-03-08},
pages = {422-427},
peerreviewed = {Yes},
title = {{Full} field digital mammography ({FFDM}) versus cmos technology, specimen radiography system ({SRS}) and tomosynthesis ({DBT}) - {Which} system can optimise surgical therapy?},
volume = {73},
year = {2013}
}
@article{faucris.264072464,
abstract = {In situ tissue engineering is an emerging field aiming at the generation of ready-to-use three-dimensional tissues. One solution to supply a proper vascularization of larger tissues to provide oxygen and nutrients is the arteriovenous loop (AVL) model. However, for this model, suitable scaffold materials are needed that are biocompatible/non-immunogenic, slowly degradable, and allow vascularization. Here, we investigate the suitability of the known gelatin methacryloyl (GelMA)-based hydrogel for in-situ tissue engineering utilizing the AVL model. Rat AVLs are embedded by two layers of GelMA hydrogel in an inert PTFE chamber and implanted in the groin. Constructs were explanted after 2 or 4 weeks and analyzed. For this purpose, gross morphological, histological, and multiphoton microscopic analysis were performed. Immune response was analyzed based on anti-CD68 and anti-CD163 staining of immune cells. The occurrence of matrix degradation was assayed by anti-MMP3 staining. Vascularization was analyzed by anti-alpha-smooth muscle actin staining, multiphoton microscopy, as well as expression analysis of 53 angiogenesis-related proteins utilizing a proteome profiler angiogenesis array kit. Here we show that GelMA hydrogels are stable for at least 4 weeks in the rat AVL model. Furthermore, our data indicate that GelMA hydrogels are biocompatible. Finally, we provide evidence that GelMA hydrogels in the AVL model allow connective tissue formation, as well as vascularization, introducing multiphoton microscopy as a new methodology to visualize neovessel formation originating from the AVL. GelMA is a suitable material for in situ and in vivo TE in the AVL model.},
author = {Heltmann-Meyer, Stefanie and Steiner, Dominik and Mueller, Claudia and Schneidereit, Dominik and Friedrich, Oliver and Salehi, Sahar and Engel, Felix and Arkudas, Andreas and Horch, Raymund E.},
doi = {10.1088/1748-605X/ac1e9d},
faupublication = {yes},
journal = {Biomedical Materials},
note = {CRIS-Team WoS Importer:2021-09-17},
peerreviewed = {Yes},
title = {{Gelatin} methacryloyl is a slow degrading material allowing vascularization and long-term use in vivo},
volume = {16},
year = {2021}
}
@article{faucris.238459074,
abstract = {The aim of this study was to analyse various gene expression profiles of muscle tissue during normoxia, ischaemia and after reperfusion in human muscle free flaps, to gain an understanding of the occurring regulatory, inflammatory and apoptotic processes on a cellular and molecular basis. Eleven Caucasian patients with soft tissue defects needing coverage with microsurgical free muscle flaps were included in this study. In all patients, the muscle samples were taken from free myocutaneous flaps. The first sample was taken before induction of ischaemia in normoxia (I), another one after ischaemia (II), and the last one was taken after reperfusion (III). The samples were analysed using DNA-microarray, real-time-quantitative-PCR and immunohistochemistry. DNA-microarray analysis detected multiple, differentially regulated genes when comparing the different groups (I-III) with statistical significance. Comparing ischaemia (II) versus normoxia (I) educed 13 genes and comparing reperfusion (III) versus ischaemia (II) educed 19 genes. The comparison of reperfusion (III) versus normoxia (I) yielded 100 differentially regulated genes. Real-time-quantitative-PCR confirmed the results of the DNA-microarrays for a subset of four genes (CASP8, IL8, PLAUR and S100A8). This study shows that ischaemia and reperfusion induces alterations on the gene expression level in human muscle free flaps. Data may suggest that the four genes CASP8, IL8, PLAUR and S100A8 are of great importance in this context. We could not confirm the DNA-microarry and real-time-quantitative-PCR results on the protein level. Finally, these findings correspond with the surgeon's clinical experience that the accepted times of ischaemia, generally up to 90 min., are not sufficient to induce pathophysiological processes, which can ultimately lead to flap loss. When inflammatory and apoptotic proteins are expressed at high levels, flap damage might occur and flap loss is likely. The sole expression on mRNA level might explain why flap loss is unlikely.},
author = {Dragu, Adrian and Schnuerer, Stefan and Surmann-Schmitt, Cordula and von der Mark, Klaus and Stürzl, Michael and Unglaub, Frank and Wolf, Maya B. and Leffler, Mareike and Beier, Justus and Kneser, Ulrich and Horch, Raymund E.},
doi = {10.1111/j.1582-4934.2010.01061.x},
faupublication = {yes},
journal = {Journal of Cellular and Molecular Medicine},
pages = {983-93},
peerreviewed = {Yes},
title = {{Gene} expression analysis of ischaemia and reperfusion in human microsurgical free muscle tissue transfer.},
volume = {15},
year = {2011}
}
@article{faucris.240326082,
author = {Promny, Dominik and Horch, Raymund E. and Agaimy, Abbas and Hauck, Theresa},
doi = {10.1055/a-1160-5886},
faupublication = {yes},
journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie},
note = {CRIS-Team WoS Importer:2020-07-10},
pages = {207-209},
peerreviewed = {unknown},
title = {{Glomustumor} am {Finger} – eine seltene, aber schmerzhafte handchirurgische {Entität}},
volume = {52},
year = {2020}
}
@article{faucris.229225249,
abstract = {Background: Guanylate binding protein-1 (GBP-1) is a large GTPase which is actively secreted by endothelial cells. It is a marker and intracellular inhibitor of endothelial cell proliferation, migration, and invasion. We previously demonstrated that stable expression of GBP-1 in murine endothelial progenitor cells (EPC) induces their premature differentiation and decreases their migration capacity in vitro and in vivo. The goal of the present study was to assess the antiangiogenic capacity of EPC expressing GBP-1 (GBP-1-EPC) and their impact on blood vessel formation in an axially vascularized 3-D bioartificial construct in vivo.Results: Functional in vitro testing demonstrated a significant increase in VEGF secretion by GBP-1-EPC after induction of cell differentiation. Undifferentiated GBP-1-EPC, however, did not secrete increased levels of VEGF compared to undifferentiated control EPC expressing an empty vector (EV-EPC). In our In vivo experiments, we generated axially vascularized tissue-engineered 3-D constructs. The new vascular network arises from an arterio-venous loop (AVL) embedded in a fibrin matrix inside a separation chamber. Total surface area of the construct as calculated from cross sections was larger after transplantation of GBP-1-EPC compared to control EV-EPC. This indicated reduced formation of fibrovascular tissue and less resorption of fibrin matrix compared to constructs containing EV-EPC. Most notably, the ratio of blood vessel surface area over total construct surface area in construct cross sections was significantly reduced in the presence of GBP-1-EPC. This indicates a significant reduction of blood vessel density and thereby inhibition of blood vessel formation from the AVL constructs caused by GBP-1. In addition, GBP-1 expressed from EPC significantly reduced cell apoptosis compared to GBP-1-negative controls.Conclusion: Transgenic EPC expressing the proinflammatory antiangiogenic GTPase GBP-1 can reduce blood vessel density and inhibit apoptosis in a developing bioartificial vascular network and may become a new powerful tool to manipulate angiogenetic processes in tissue engineering and other pathological conditions such as tumour angiogenesis. © 2012 Bleiziffer et al.; licensee BioMed Central Ltd.},
author = {Bleiziffer, Oliver and Hammon, Matthias and Arkudas, Andreas and Taeger, Christian and Beier, Justus and Amann, Kerstin Ute and Naschberger, Elisabeth and Stürzl, Michael and Horch, Raymund E. and Kneser, Ulrich},
doi = {10.1186/1472-6750-12-94},
faupublication = {yes},
journal = {BMC Biotechnology},
keywords = {Angiogenesis; Endothelial progenitor cells; Guanylate-binding protein 1; In vivo tissue engineering},
note = {Created from Fastlane, Scopus look-up},
peerreviewed = {Yes},
title = {{Guanylate}-binding protein 1 expression from embryonal endothelial progenitor cells reduces blood vessel density and cellular apoptosis in an axially vascularised tissue-engineered construct},
volume = {12},
year = {2012}
}
@article{faucris.238463921,
abstract = {Reversely transfected cell microarrays (RTCM) have been introduced as a method for parallel high throughput analysis of gene functions in mammalian cells. Hundreds to thousands of different recombinant DNA or RNA molecules can be transfected into different cell clusters at the same time on a single glass slide with this method. This allows either the simultaneous overexpression or--by using the recently developed RNA interference (RNAi) techniques--knockdown of a huge number of target genes. A growing number of sophisticated detection systems have been established to determine quantitatively the effects of the transfected molecules on the cell phenotype. Several different cell types have been successfully used for this procedure. This review summarizes the presently available knowledge on this technique and provides a laboratory protocol.},
author = {Stürzl, Michael and Konrad, Andreas and Sander, Gaby and Wies, Effi and Neipel, Frank and Naschberger, Elisabeth and Reipschlaeger, Simone and Gonin-Laurent, Nathalie and Horch, Raymund E. and Kneser, Ulrich and Hohenberger, Werner and Erfle, Holger and Thurau, Mathias},
doi = {10.2174/138620708783744499},
faupublication = {yes},
journal = {Combinatorial Chemistry & High Throughput Screening},
pages = {159-72},
peerreviewed = {Yes},
title = {{High} throughput screening of gene functions in mammalian cells using reversely transfected cell arrays: review and protocol.},
volume = {11},
year = {2008}
}
@article{faucris.233241238,
abstract = {Lymphedema is a chronic progressive disease ultimately resulting in severe, disfiguring swelling and permanent changes of the affected tissues. Presently, there is no causal treatment approach of lymphedema. Therefore, most therapies are purely symptomatic. However, the recent use of stem cell-based therapies has offered new prospects for alternative treatment options. The present study was performed to investigate the effects of human adipose-derived stem cells (ADSCs) on human dermal lymphatic endothelial cells (HDLECs) in terms of basic in vitro lymphangiogenic assays (WST-8 assay, scratch assay, transmigration assay, sprouting assay, tube formation assay). The influence of ADSC-conditioned medium (ADSC-CM) on HDLECs was compared to recombinant VEGF-C, bFGF and HGF. Further ADSC-CM was characterized by protein microarray and enzyme-linked immunosorbent assay (ELISA). Although key-lymphangiogenic growth factors - like VEGF-C - could only be detected in low concentrations within the conditioned medium (CM), HDLECs were potently stimulated to proliferate, migrate and to form tube like structures by ADSC-CM. Despite concentrations more than hundredfold higher than those found in the conditioned medium, stimulation with recombinant VEGF-C, bFGF and HGF was still weaker compared to ADSC-CM. These results highlight the effectiveness of growth factors secreted by ADSC to stimulate HDLEC, potentially providing a promising new therapeutic approach for the treatment of lymphedema.},
author = {Ahmadzadeh, Nima and Robering, Jan Willem and Kengelbach-Weigand, Annika and Al-Abboodi, Majida and Beier, Justus and Horch, Raymund E. and Boos, Anja},
doi = {10.1016/j.yexcr.2020.111816},
faupublication = {yes},
journal = {Experimental Cell Research},
keywords = {Adipose-derived stem cells (ADSCs); Cell therapy; Human dermal lymphatic endothelial cells (HDLECs); Lymphangiogenesis; Lymphedema; Secretome},
note = {CRIS-Team Scopus Importer:2020-02-04},
peerreviewed = {Yes},
title = {{Human} adipose-derived stem cells support lymphangiogenesis in vitro by secretion of lymphangiogenic factors},
year = {2020}
}
@article{faucris.244325710,
abstract = {Introduction:For the regeneration of large volume tissue defects, the interaction between angiogenesis and osteogenesis is a crucial prerequisite. The surgically induced angiogenesis by means of an arteriovenous loop (AVL), is a powerful methodology to enhance vascularization of osteogenic matrices. Moreover, the AVL increases oxygen and nutrition supply, thereby supporting cell survival as well as tissue formation. Adipose-derived stem cells (ADSCs) are interesting cell sources because of their simple isolation, expansion, and their osteogenic potential. This study targets to investigate the coimplantation of human ADSCs after osteogenic differentiation and human umbilical vein endothelial cells (HUVECs), embedded in a vascularized osteogenic matrix of hydroxyapatite (HAp) ceramic for bone tissue engineering. Materials and Methods:An osteogenic matrix consisting of HAp granules and fibrin has been vascularized by means of an AVL. Trials in experimental groups of four settings were performed. Control experiments without any cells (A) and three cell-loaded groups using HUVECs (B), ADSCs (C), as well as the combination of ADSCs and HUVECs (D) were performed. The scaffolds were implanted in a porous titanium chamber, fixed subcutaneously in the hind leg of immunodeficient Rowett Nude rats and explanted after 6 weeks. Results:In all groups, the osteogenic matrix was strongly vascularized. Moreover, remodeling processes and bone formation in the cell-containing groups with more bone in the coimplantation group were proved successful. Conclusion:Vascularization and bone formation of osteogenic matrices consisting of ADSCs and HUVECs in the rat AVL model could be demonstrated successfully for the first time. Hence, the coimplantation of differentiated ADSCs with HUVECs may therefore be considered as a promising approach for bone tissue engineering. Impact statement Due to their low donor-site morbidity, osteogenic potential and their good cell expansion, human adipose-derived stem cell is an interesting cell source. The goal of this study is to transfer thein vitroresults of increasing osteogenic potential upon cocultivation with human umbilical vein endothelial cell, to the rat arteriovenous loop model within the next step. Without using growth factors, the biomaterials, fibrin and HAp, which have already been successfully applied in clinical practice, are supposed to support bone formation by the implanted cells. The results demonstrated that vascularized bone tissue was formed in the cell-containing groups after 6 weeks.},
author = {Winkler, Sophie and Mutschall, Hilkea and Biggemann, Jonas and Fey, Tobias and Greil, Peter and Körner, Carolin and Weisbach, Volker Günter and Meyer-Lindenberg, Andrea and Arkudas, Andreas and Horch, Raymund E. and Steiner, Dominik},
doi = {10.1089/ten.tea.2020.0087},
faupublication = {yes},
journal = {Tissue Engineering - Part A},
note = {CRIS-Team WoS Importer:2020-10-23},
peerreviewed = {Yes},
title = {{Human} {Umbilical} {Vein} {Endothelial} {Cell} {Support} {Bone} {Formation} of {Adipose}-{Derived} {Stem} {Cell}-{Loaded} and {3D}-{Printed} {Osteogenic} {Matrices} in the {Arteriovenous} {Loop} {Model}},
year = {2020}
}
@article{faucris.122632884,
abstract = {In the field of free flap transfer in reconstructive surgery, the trans- or replanted tissue always undergoes cell damage during ischemia to a more or less strong extent. In previous studies we already showed that conserving muscle transplants by means of extracorporeal perfusion over a period of 6 hours by using a crystalloid solution for perfusion. However, we observed significant edema formation. In this study we aimed at reducing the edema formation by using an iso-oncotic colloid as perfusion solution. This way we want to evaluate a possible new application of hydroxyl-ethyl starch in an extracorporeal setup to exploit potential benefits of the colloid.Examined parameters include the muscles' functionality with external field stimulation, histological examination and edema formation. Perfused muscles showed a statistically significant higher ability to exert force compared to nonperfused ones. These findings can be confirmed using Annexin V as marker for cell damage, perfusion of muscle tissue limits damage significantly compared to nonperfused tissue. Substituting the electrolyte perfusion solution with a colloidal one shows the tendency to reduce the edema formation but without statistical significance.},
author = {Taeger, Christian and Friedrich, Oliver and Drechsler, Caroline and Weigand, Annika and Hobe, Frieder and Geppert, Carol-Immanuel and Münch, Frank and Birkholz, Torsten and Buchholz, Rainer and Horch, Raymund E. and Präbst, Konstantin and Buchholz, Rainer},
doi = {10.3233/CH-162049},
faupublication = {yes},
journal = {Clinical hemorheology and microcirculation},
peerreviewed = {Yes},
title = {{Hydroxyethyl} starch solution for extracorporeal tissue perfusion},
year = {2016}
}
@article{faucris.269959974,
abstract = {Ionizing radiation has become an integral part of modern cancer therapy regimens. Various side effects, such as radiation dermatitis, affect patients in acute and chronic forms and decrease therapy compliance significantly. In this study, primary keratinocytes were irradiated in a 2-dimensional (2D) culture as well as on a 3-dimensional (3D) collagen-elastin matrix with doses of 2 and 5 Gy. The effect of different concentrations of IGF-I, KGF, platelet lysate (PL), high and low molecular weight hyaluronic acid (H-HA, L-HA), and adipose-derived stem cell (ADSC) conditioned medium was analyzed in respect to cell viability (WST-8), wound closure (migration), and the gene expression (quantitative real-time PCR) of 2D cultures. The 3D culture was evaluated by WST-8. A mixture of H-HA and L-HA, as well as IGF-I, could significantly stimulate the keratinocyte viability and migration which were severely reduced by irradiation. The MKI67and IL6 gene expression of irradiated keratinocytes was significantly higher after H-HA/L-HA treatment. The stimulating effects of H-HA/L-HA and IGF-I were able to be confirmed in 3D culture. A positive influence on cell viability, migration, and gene expression was achieved after the treatment with H-L-HA and IGF-I. These results open the possibility of a novel therapeutic method for both the prevention and the treatment of radiation dermatitis.},
author = {Sörgel, Celena and Schmid, Rafael and Stadelmann, Nina and Weisbach, Volker Günter and Distel, Luitpold and Horch, Raymund E. and Kengelbach-Weigand, Annika},
doi = {10.3390/cancers14030588},
faupublication = {yes},
journal = {Cancers},
keywords = {Dermatitis; Hyaluronic acid; IGF-I; Irradiation; Keratinocytes; Wound healing},
note = {CRIS-Team Scopus Importer:2022-02-25},
peerreviewed = {Yes},
title = {{IGF}-{I} and {Hyaluronic} {Acid} {Mitigate} the {Negative} {Effect} of {Irradiation} on {Human} {Skin} {Keratinocytes}},
volume = {14},
year = {2022}
}
@article{faucris.246973839,
author = {Boos, Anja M. and Ludolph, Ingo and Horch, Raymund E.},
doi = {10.1055/a-1217-0985},
faupublication = {yes},
journal = {Phlebologie},
note = {CRIS-Team WoS Importer:2020-12-18},
peerreviewed = {unknown},
title = {{Imaging} {Diagnostics} for {Therapy} {Planning} for {Lipodema} and {Lymphodema} {Reply}},
year = {2020}
}
@article{faucris.234228959,
abstract = {Lipoedema is a progressive disease, which predominantly affects women. It is characterised by circumferential growth, with increase in fat tissue of the extremities, and can lead to oedema. In contrast, the lymphoedema is defined by a specific lymphatic drainage disorder and can lead to fibrosis of the surrounding connective tissue. While lipoedema is diagnosed through clinical symptoms and diagnostic imaging can usually only be used to rule out comorbidities, lymphatic drainage disorder can be visualised using imaging methods. Ultrasound is a basis diagnostic tool to show retained interstitial fluid in lip- and lymphoedema. Lymphoscintigraphy is considered to be the gold standard to demonstrate a lymphatic drainage disorder, which can be combined with computed tomography. Indocyanine green(ICG) lymphography is a dynamic imaging tool, which is of increasing significance due to its possible intraoperative use. Magnetic resonance imaging (MRI) lymphography has the ability to visualise a lymphoedema and a lymphatic drainage disorder in three dimensions and is therefore considered to be a meaningful supplement. Therapy of lip- and lymphoedema should always be based on a combination of conservative and surgical strategies. While liposuction and other resection procedures can lead to symptom relief in lipedoema or advanced lymphoedema, newer reconstructive procedures such as lymph node transplantation or lymphovenous anastomoses are modern techniques to treat lymphoedema. A long lasting volume reduction can be achieved by creating new lymphatic pathways leading to improved quality of life and reduced symptoms.},
author = {Zetzmann, Katharina and Ludolph, Ingo and Horch, Raymund E. and Boos, Anja},
doi = {10.1055/a-0739-7911},
faupublication = {yes},
journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie},
note = {EVALuna2:211463},
pages = {386-392},
peerreviewed = {Yes},
title = {{Imaging} for treatment planning in lipo-and lymphedema},
volume = {50},
year = {2018}
}
@article{faucris.123084104,
abstract = {In recent years, a controversial discussion about the clinical relevance of the angiosome concept during tibial angioplasty has developed. Therefore, we conducted a prospective study to evaluate the angiosome concept on the level of microcirculation during tibial vascular interventions.Thirty patients with isolated tibial angioplasty were examined prospectively. Macrocirculation was evaluated by measurement of the ankle-brachial index (ABI). For the assessment of microcirculation, a combined method of laser Doppler flowmetry and tissue spectrometry (O2C; LEA Medizintechnik GmbH, Giessen, Germany) was applied. Microcirculatory parameters were measured continuously during the procedures. Measuring points were located over different angiosomes of the index foot; a control probe was placed on the contralateral leg.Cumulated microcirculation parameters (sO2, flow) as well as the ABI showed a significant improvement postinterventionally (ABI, P < .001; sO2, P < .001; flow, P < .001). Assessment of the separate angiosomes of the index leg and the comparison of the directly revascularized (DR) and indirectly revascularized (IR) angiosomes showed no significant difference concerning the microperfusion postinterventionally (DR - IR: sO2, P = .399; flow, P = .909) as well as during angioplasty. Even a further subdivision of the collective into patients with diabetes (sO2, P = .445; flow, P =.758) and renal insufficiency (sO2, P = .246; flow, P = .691) could not demonstrate a superiority of the direct revascularization at the level of microcirculation in these patients (comparison DR - IR).There is a significant overall improvement in tissue perfusion of the foot immediately after tibial angioplasty. The effect shown in this study, however, was found to be global and was not restricted to certain borders, such as defined by angiosomes.},
author = {Rother, Ulrich and Krenz, Katrin and Lang, Werner and Horch, Raymund E. and Schmid, Axel and Heinz, Marco and Meyer, Alexander and Regus, Susanne},
doi = {10.1016/j.jvs.2016.08.099},
faupublication = {yes},
journal = {Journal of Vascular Surgery},
note = {EVALuna2:14616},
pages = {422-430},
peerreviewed = {Yes},
title = {{Immediate} changes of angiosome perfusion during tibial angioplasty},
volume = {65},
year = {2017}
}
@article{faucris.271004978,
abstract = {The microvascular endothelial network plays an important role in osteogenesis, bone regeneration and bone tissue engineering. Endothelial progenitor cells (EPCs) display a high angiogenic and vasculogenic potential. The endothelialization of scaffolds with endothelial progenitor cells supports vascularization and tissue formation. In addition, EPCs enhance the osteogenic differentiation and bone formation of mesenchymal stem cells (MSCs). This study aimed to investigate the impact of EPCs on vascularization and bone formation of a hydroxyapatite (HA) and beta-tricalcium phosphate (ß-TCP)–fibrin scaffold. Three groups were designed: a scaffold-only group (A), a scaffold and EPC group (B), and a scaffold and EPC/MSC group (C). The HA/ß–TCP–fibrin scaffolds were placed in a porous titanium chamber permitting extrinsic vascularization from the surrounding tissue. Additionally, intrinsic vascularization was achieved by means of an arteriovenous loop (AV loop). After 12 weeks, the specimens were explanted and investigated by histology and CT. We were able to prove a strong scaffold vascularization in all groups. No differences regarding the vessel number and density were detected between the groups. Moreover, we were able to prove bone formation in the coimplantation group. Taken together, the AV loop is a powerful tool for vascularization which is independent from scaffold cellularization with endothelial progenitor cells’ prior implantation.},
author = {Steiner, Dominik and Reinhardt, Lea and Fischer, Laura and Popp, Vanessa and Körner, Carolin and Geppert, Carol-Immanuel and Bäuerle, Tobias and Horch, Raymund E. and Arkudas, Andreas},
doi = {10.3390/cells11060926},
faupublication = {yes},
journal = {Cells},
keywords = {AV loop; Bone tissue engineering; Endothelial progenitor cell; Mesenchymal stem cell; Vascularization},
note = {CRIS-Team Scopus Importer:2022-03-18},
peerreviewed = {Yes},
title = {{Impact} of {Endothelial} {Progenitor} {Cells} in the {Vascularization} of {Osteogenic} {Scaffolds}},
volume = {11},
year = {2022}
}
@article{faucris.242391057,
abstract = {Skin temperature plays a certain role in the dermal absorption of substances, but the extent and mechanisms of skin temperatures-induced modulation in ranges caused by physiological thermoregulation or environmental conditions are largely unknown. The influence of dermal temperature on the absorption of the model lipophilic compound (anisole) and the model hydrophilic compounds (1,4-dioxane, ethanol) through human skin was investigated at three dermal temperatures (25, 32 and 39 °C) in an ex-vivo diffusion cell model. The substances were applied to the skin and transdermal penetration was monitored. All substances showed temperature dependent variations in their penetration behavior (3 h: 25–39 °C: 202–275% increase in cumulative, transdermally penetrated amounts). The relative differences in absorption in relation to temperature were greatest within 45 min after exposure (25–39 °C: 347–653% rise in cumulated penetration), although absolute amounts absorbed were small (45 min vs. 3 h: 4.5–14.5%). Regardless of blood circulation, skin temperature significantly influences the amount and kinetics of dermal absorption. Substance-dependent, temperature-related changes of the lipid layer order or the porous pathway may facilitate penetration. The early-stage modulation of transdermal penetration indicates transappendageal absorption, which may be relevant for short-term exposures. For both, toxicological evaluation and perfusion cell studies, it is important to consider the thermal influence on absorption or to perform the latter at a standardized temperature (32±1 °C).},
author = {Kilo, Sonja and Wick, J. and Mini Vijayan, Suvarna and Göen, Thomas and Horch, Raymund E. and Ludolph, Ingo and Drexler, Hans},
doi = {10.1016/j.tiv.2020.104954},
faupublication = {yes},
journal = {Toxicology in Vitro},
keywords = {Dermal absorption; Diffusion cell; Influence of temperature; Transdermal penetration},
note = {CRIS-Team Scopus Importer:2020-09-11},
peerreviewed = {Yes},
title = {{Impact} of physiologically relevant temperatures on dermal absorption of active substances - an ex-vivo study in human skin},
volume = {68},
year = {2020}
}
@article{faucris.276687224,
abstract = {Background: Deep inferior epigastric perforator and muscle sparing transverse rectus abdominis muscle flaps are commonly used flaps for autologous breast reconstruction. CT-angiography allows to analyse the perforator course preoperatively. Our aim was to compare the different aspects of perforator anatomy in the most detailed study. Methods: CT-angiographies of 300 female patients with autologous breast reconstruction of 10 years were analysed regarding the anatomy of the deep inferior epigastric artery and every perforator. Results: Overall, 2260 perforators were included. We identified correlations regarding the DIEA branching point and number of perforators and their intramuscular course. The largest perforator emerged more often from the medial branch of the DIEA than the smaller perforators (70% (416/595) vs. 54% (878/1634), p < 0.001) and more often had a direct connection to the SIEV (large 67% (401/595) vs. small 39% (634/1634), p < 0.01). Medial row perforators were larger than the laterals (lateral 1.44 mm ± 0.43 (n = 941) vs. medial 1.58 mm ± 0.52 (n = 1304) (p < 0.001)). The larger and more medial the perforator, the more likely it was connected to the SIEV: perforators with direct connection to the SIEV had a diameter of 1.65 mm ± 0.53 (n = 1050), perforators with indirect connection had a diameter of 1.43 ± 0.43 (n = 1028), perforators without connection had a diameter of 1.31 mm ± 0.37 (n = 169) (p < 0.001). Medial perforators were more often directly connected to the SIEV than lateral perforators (medial 56% (723/1302) vs. lateral 35% (327/941), p < 0.001). A lateral perforator more often had a short intramuscular course than medial perforators (69% (554/800) vs. 45% (474/1055), p < 0.001), which was also more often observed in the case of a small perforator and a caudal exit of the rectus sheath. Conclusion: The largest perforator emerges more often from the medial branch of the DIEA and frequently has a direct connection to the SIEV, making medial row perforators ideal for DIEP flap transplantation.},
author = {Frank, Katharina and Stroebel, Armin and Ludolph, Ingo and Hauck, Theresa and May, Matthias and Beier, Justus and Horch, Raymund E. and Arkudas, Andreas},
doi = {10.3390/jpm12050701},
faupublication = {yes},
journal = {Journal of Personalized Medicine},
keywords = {autologous breast reconstruction; CTA; DIEP flap; MS-TRAM flap; perforator},
note = {CRIS-Team Scopus Importer:2022-06-10},
peerreviewed = {Yes},
title = {{Improving} the {Safety} of {DIEP} {Flap} {Transplantation}: {Detailed} {Perforator} {Anatomy} {Study} {Using} {Preoperative} {CTA}†},
volume = {12},
year = {2022}
}
@article{faucris.277311801,
abstract = {Wound closure is a key element of any procedure, especially aesthetic and reconstructive plastic surgery. Therefore, over the last decades, several devices have been developed in order to assist surgeons in achieving better results while saving valuable time. In this work, we give a concise review of the literature and present a biomechanical study of different suturing materials under mechanical load mimicking handling in the operating theatre. Nine different suture products, all of the same USP size (4-0), were subjected to a standardized crushing load by means of a needle holder. All materials were subjected to 0, 1, 3 and 5 crushing load cycles, respectively. The linear tensile strength was measured by means of a universal testing device. Attenuation of tensile strength was evaluated between materials and between crush cycles. In the pooled analysis, the linear tensile strength of the suture materials deteriorated significantly with every cycle (p < 0.0001). The suture materials displayed different initial tensile strengths (in descending order: polyglecaprone, polyglactin, polydioxanone, polyamid, polypropylene). In comparison, materials performed variably in terms of resistance to crush loading. The findings were statistically significant. The reconstructive surgeon has to be flexible and tailor wound closure techniques and materials to the individual patient, procedure and tissue demands; therefore, profound knowledge of the physical properties of the suture strands used is of paramount importance. The crushing load on suture materials during surgery can be detrimental for initial and long-term wound repair strength. As well as the standard wound closure methods (sutures, staples and adhesive strips), there are promising novel devices.
Implantation of breast prosthesis is still one of the most frequently performed breast reconstructing or contouring procedures.
Infectious complications and capsular contracture are inherent problems that may have different causes which are not clearly defined yet in terms of pathophysiology. Recent findings showed bacterial contamination as a major cause of implant failure. Since this has direct implications for the surgical management we report on biofilm development on alloplastic breast prostheses, characteristics and effects after implantation of medical devices in general. This article gives a review of the current literature and discusses possible issues to solve the problem of infection after implantation of breast prosthesis.
In conclusion the reinsertion of single-use devices should not be recommended and should be strictly avoided when a device related infection has occured. According to current knowledge contaminated implants should be removed, the infection then be cured and if necessary, a new prosthesis may be implanted after a regeneration period. Alternatively a change in therapy towards autologous tissue reconstruction should be considered if previous attempts with alloplastic prostheses have failed and if radiation therapy has worsened the local tissue situation in the recipient area.
}, author = {Horch, Raymund E. and Schultz, Gregory and Schubert, Dirk W. and Schmitz, Marweh}, doi = {10.3205/gpras000014}, faupublication = {yes}, journal = {GMS German Plastic, Reconstructive and Aesthetic Surgery - Burn and Hand Surgery}, peerreviewed = {Yes}, title = {{Infectious} complications in implant based breast surgery and implications for plastic surgeons}, year = {2013} } @inproceedings{faucris.121400224, author = {Daenicke, Jonas and Schubert, Dirk W. and Gedde, Ulf and Hedenqvist, Mikael and Linde, Erik and Sigl, Thomas and Horch, Raymund E.}, booktitle = {Programme and Book of Abstracts}, date = {2016-06-19/2016-06-23}, faupublication = {yes}, pages = {121}, peerreviewed = {unknown}, title = {{Influence} of crosslinking and penetrant size on the diffusion properties of cyclic siloxanes through silicone elastomers}, venue = {Stockholm}, year = {2016} } @article{faucris.111066824, abstract = {INTRODUCTION Aim of the present study was the establishment of an efficient and reproducible model for irradiation of rat femora as a model for impaired osteogenesis and angiogenesis. MATERIALS AND METHODS Four different irradiation protocols were compared: single irradiation of the left femur with 20 Gy and explantation after 4 or 8 weeks (group A, B); three irradiation fractions at 3-4 days intervals with 10 Gy and explantation after 4 or 8 weeks (group C, D). The contralateral, unirradiated femur served as control. Evaluation included histology, microcomputertomography (μCT) and real time PCR. RESULTS Histology showed a pronounced increase of vacuoles in bone marrow after irradiation, especially after 4 weeks (group A and C), demonstrating bone marrow edema and fatty degeneration. Irradiation provoked a decrease of total cell numbers in cortical bone and of Hif1α positive cells in bone marrow. The expression of several markers (osteocalcin, RUNX2, TGFβ1, TNFα, VEGF-A, Hif1α) was decreased in group A after irradiation. This might suggest a decreased metabolism after irradiation. A significant decrease in small-sized vessels was seen in μCT evaluation in group A and D. CONCLUSIONS Single irradiation with 20 Gy had the most severe and reproducible impact on osteogenesis and angiogenesis after 4 weeks while being well tolerated by all animals, thus making it an excellent model for evaluation of bone healing and vascularization in irradiated tissu}, author = {Rottensteiner-Brandl, Ulrike and Distel, Luitpold and Stumpf, Martin and Fey, Tobias and Köhn, Katrin and Bertram, Ulf and Lingens, Lara and Greil, Peter and Horch, Raymund E. and Arkudas, Andreas}, doi = {10.1089/ten.TEC.2017.0170}, faupublication = {yes}, journal = {Tissue Engineering - Part C: Methods}, keywords = {irradiation model; bone tissue engineering; angiogenesis; osteogenesis}, pages = {583-591}, peerreviewed = {Yes}, title = {{Influence} of different irradiation protocols on vascularization and bone formation parameters in rat femora.}, volume = {23}, year = {2017} } @inproceedings{faucris.243335859, address = {HEIDELBERG}, author = {Sörgel, Celena and Fietkau, Rainer and Distel, Luitpold}, booktitle = {STRAHLENTHERAPIE UND ONKOLOGIE}, faupublication = {yes}, note = {CRIS-Team WoS Importer:2020-10-02}, pages = {S151-S151}, peerreviewed = {unknown}, publisher = {SPRINGER HEIDELBERG}, title = {{Influence} of neoadjuvant {Radiation} {Therapy} on the {Quality} of {Life} in {Patients} with advanced {Rectal} {Cancer} over a {Period} of 5 {Years}}, venue = {Weisbaden}, year = {2020} } @article{faucris.273197522, abstract = {Previous studies provide high evidence that autotaxin (ATX)-lysophosphatidic acid (LPA) signaling through LPA receptors (LPAR) plays an important role in breast cancer initiation, progression, and invasion. However, its specific role in different breast cancer cell lines remains to be fully elucidated to offer improvements in targeted therapies. Within this study, we analyzed in vitro the effect of LPA 18:1 and the LPAR1, LPAR3 (and LPAR2) inhibitor Ki16425 on cellular functions of different human breast cancer cell lines (MDA-MB-231, MDA-MB-468, MCF-7, BT-474, SKBR-3) and the human breast epithelial cell line MCF-10A, as well as Interleukin 8 (IL-8), Interleukin 6 (IL-6) and tumor necrosis factor (TNF)-alpha cytokine secretion after LPA-incubation. ATX-LPA signaling showed a dose-dependent stimulatory effect especially on cellular functions of triple-negative and luminal A breast cancer cell lines. Ki16425 inhibited the LPA-induced stimulation of triple-negative breast cancer and luminal A cell lines in variable intensity depending on the functional assay, indicating the interplay of different LPAR in those assays. IL-8, IL-6 and TNF-alpha secretion was induced by LPA in MDA-MB-468 cells. This study provides further evidence about the role of the ATX-LPA axis in different breast cancer cell lines and might contribute to identify subtypes suitable for a future targeted therapy of the ATX-LPA axis.}, author = {Hauck, Theresa and Kadam, Sheetal and Heinz, Katharina and Garcia Peraza, Maria and Schmid, Rafael and Kremer, Andreas and Wolf, Katharina and Bauer, Alina and Horch, Raymund E. and Arkudas, Andreas and Kengelbach-Weigand, Annika}, doi = {10.1038/s41598-022-09565-3}, faupublication = {yes}, journal = {Scientific Reports}, keywords = {Cancer; Medical research; Molecular medicine}, note = {CRIS-Team Scopus Importer:2022-04-15}, peerreviewed = {Yes}, title = {{Influence} of the autotaxin-lysophosphatidic acid axis on cellular function and cytokine expression in different breast cancer cell lines}, volume = {12}, year = {2022} } @article{faucris.122850684, author = {Horch, Raymund E.}, doi = {10.1111/jcmm.12686}, faupublication = {yes}, journal = {Journal of Cellular and Molecular Medicine}, note = {EVALuna2:15587}, pages = {2047-8}, peerreviewed = {No}, title = {{In} {Memoriam}: {Professor} {Laurentiu} {M}. {Popescu} (1944-2015)}, volume = {19}, year = {2015} } @article{faucris.238885928, abstract = {Relapsing or far advanced rectal and anal cancers remain difficult to treat and require interdisciplinary approaches. Due to modern standard protocols all patients receive irradiation and neoadjuvant chemotherapy—and in case of a relapse a second irradiation—rendering the surgical site prone to surgical site infections and oftentimes long lasting sinus and septic complications after exenteration in the pelvis. Despite an improved overall survival rate in these patients the downside of radical tumor surgery in the pelvis is a major loss of quality of life, especially in women when parts of the vagina need to be resected. Derived from our experince with over 300 patients receiving pelvic and perineal reconstruciton with a transpelvic vertical rectus abdominis myocutaneous (tpVRAM) flap we studied the impact of this surgical technique on the outcomes of female patients with or without vaginal reconstruction following pelvic exenteration. We found out that the tpVRAM flap is reliably perfused and helps to reduce long term wound healing desasters in the irradiated perineal/vaginal/gluteal region.}, author = {Horch, Raymund E. and Ludolph, Ingo and Weber, Klaus and Grützmann, Robert and Arkudas, Andreas and Cai, Aijia}, doi = {10.3389/fonc.2020.00719}, faupublication = {yes}, journal = {Frontiers in Oncology}, keywords = {anal cancer; rectal cancer; transpelvic vertical rectus abdominis myocutaneous flap; vaginal reconstruction; VRAM}, note = {CRIS-Team Scopus Importer:2020-06-02}, peerreviewed = {Yes}, title = {{Interdisciplinary} {Surgical} {Approaches} in {Vaginal} and {Perineal} {Reconstruction} of {Advanced} {Rectal} and {Anal} {Female} {Cancer} {Patients}}, volume = {10}, year = {2020} } @article{faucris.291587931, abstract = {Soft-tissue sarcomas (STS) are rare, but potentially life-threatening malignancies. STS can occur anywhere in the human body with the limbs being the most common site. Referral to a specialized sarcoma center is crucial to guarantee prompt and appropriate treatment. STS treatment strategies should be discussed in an interdisciplinary tumor board to involve expertise from all available resources, including an experienced reconstructive surgeon for an optimal outcome. In many cases, extensive resection is needed to achieve R0 resection, resulting in large defects after surgery. Hence, an evaluation of whether plastic reconstruction might be required is mandatory to avoid complications due to insufficient primary wound closure. In this retrospective observational study, we present data of patients with extremity STS treated at the Sarcoma Center, University Hospital Erlangen, in 2021. We found that complications were more frequent in patients who received secondary flap reconstruction after insufficient primary wound closure compared to patients who received primary flap reconstruction. Additionally, we propose an algorithm for an interdisciplinary surgical therapy of soft-tissue sarcomas regarding resection and reconstruction and present two problematic cases to emphasize the complexity of surgical sarcoma therapy.}, author = {Osterloh, Justus and Ludolph, Ingo and Grützmann, Robert and Meyer, Alexander and Lang, Werner and Horch, Raymund E. and Fechner, Katja and Arkudas, Andreas}, doi = {10.3390/jpm13020262}, faupublication = {yes}, journal = {Journal of Personalized Medicine}, keywords = {extremity soft-tissue sarcomas; reconstructive surgery; surgical resection}, note = {CRIS-Team Scopus Importer:2023-03-10}, peerreviewed = {Yes}, title = {{Interdisciplinary} {Surgical} {Therapy} of {Extremity} {Soft}-{Tissue} {Sarcomas}: {A} {Personalized} {Resection} and {Reconstruction} {Algorithm}}, volume = {13}, year = {2023} } @article{faucris.123733984, author = {Boos, Anja and Beckmann, Matthias and Horch, Raymund E. and Beier, Justus}, doi = {10.1055/s-0034-1368425}, faupublication = {yes}, journal = {Geburtshilfe und Frauenheilkunde}, note = {EVALuna2:15567}, pages = {574-578}, peerreviewed = {Yes}, title = {{Interdisciplinary} {Treatment} for {Cutaneous} {Abdominal} {Wall} {Metastasis} from {Cervical} {Cancer} with {Resection} and {Reconstruction} of the {Abdominal} {Wall} {Using} {Free} {Latissimus} {Dorsi} {Muscle} {Flap}: {A} {Case} {Report}}, volume = {74}, year = {2014} } @article{faucris.237178574, abstract = {Background: Breast cancer is the most common malignancy in women. The interdisciplinary treatment is based on the histological tumor type, the TNM classification, and the patient's wishes. Following tumor resection and (neo-) adjuvant therapy strategies, breast reconstruction represents the final step in the individual interdisciplinary treatment plan. Although manifold flaps have been described, abdominal free flaps, such as the deep inferior epigastric artery perforator (DIEP) or the muscle-sparing transverse rectus abdominis myocutaneous (ms-TRAM) flap, are the current gold standard for autologous breast reconstruction. This retrospective study focuses on the safety of autologous breast reconstruction upon mastectomy using abdominal free flaps. Methods: From April 2012 until December 2018, 193 women received 217 abdominal free flaps for autologous breast reconstruction at the University Hospital of Erlangen. For perforator mapping, we performed computed tomography angiography (CTA). Venous anastomosis was standardized using a ring pin coupler system, and flap perfusion was assessed with fluorescence angiography. A retrospective analysis was performed based on medical records, the surgery report, and follow-up of outpatient course. Results: In most cases, autologous breast reconstruction was performed as a secondary reconstructive procedure after mastectomy and radiotherapy. In total, 132 ms1-TRAM, 23 ms2-TRAM, and 62 DIEP flaps were performed with 21 major complications (10%) during hospital stay including five free flap losses (2.3%). In all cases of free flap loss, we found an arterial thrombosis as the main cause. In 24 patients a bilateral breast reconstruction was performed without free flap loss. The majority of free flaps (96.7%) did not need additional supercharging or turbocharging to improve venous outflow. Median venous coupler size was 2.5 mm (range, 1.5–3.5 mm). Conclusion: Using CTA, intraoperative fluorescence angiography, titanized hernia meshes for rectus sheath reconstruction, and venous coupler systems, autologous breast reconstruction with DIEP or ms-TRAM free flaps is a safe and standardized procedure in high-volume microsurgery centers.}, author = {Steiner, Dominik and Horch, Raymund E. and Ludolph, Ingo and Schmitz, Marweh and Beier, Justus and Arkudas, Andreas}, doi = {10.3389/fonc.2020.00177}, faupublication = {yes}, journal = {Frontiers in Oncology}, keywords = {breast reconstruction; CTA; DIEP; interdisciplinary; ms-TRAM; venous coupler}, note = {CRIS-Team Scopus Importer:2020-04-10}, peerreviewed = {Yes}, title = {{Interdisciplinary} {Treatment} of {Breast} {Cancer} {After} {Mastectomy} {With} {Autologous} {Breast} {Reconstruction} {Using} {Abdominal} {Free} {Flaps} in a {University} {Teaching} {Hospital}—{A} {Standardized} and {Safe} {Procedure}}, volume = {10}, year = {2020} } @article{faucris.108963624, author = {Schmitz, Marweh and Sirbu, Horia and Horch, Raymund E.}, doi = {10.1007/s00104-015-0002-3}, faupublication = {yes}, journal = {Chirurg}, note = {EVALuna2:15589}, pages = {889-91}, peerreviewed = {Yes}, title = {{Interdisciplinary} treatment of extensive chest wall defects due to irradiation}, volume = {86}, year = {2015} } @article{faucris.255845121, abstract = {Background Plastic and Reconstructive surgery is able to treat patients from head to toe, whereby it can preserve, reform or reconstruct anatomically functional or aesthetic unions. This kind of expertise is appreciated by other medical specialties and is part of high performance medicine in most university clinics. However the standing of academic Plastic Surgery is particularly difficult. In this study we highlight the academic and clinical interdisciplinarity of German academic Plastic Surgery. Methods By creating a questionnaire we evaluated educational assignments of academic Plastic Surgery and its interdisciplinary surgical performance at German university hospitals. Results In 12 of 36 university hospitals there is no kind of Plastic Surgery established. There are 11 university clinics, 5 departments, 4 sections and 4 consultants with Plastic Surgery expertise. 13 university centers took part of investigation. All of them are integrated in the educational curriculum with high discrepancy (2-12 hours per semester). Discrepancy seems not to be affect by clinic independence or team size. 10 of 13 university center wants to augment educational efforts. In 2015 participating 13 university hospitals treated 1476 interdisciplinary patients. In 1165 cases there was no invoicing of services. Conclusion Plastic Surgery is an integral part of high performance medicine, e. g. in traumatological, oncologic or infectious cases. The complex reconstructive procedures are completing successful therapy of other specialties. Missing of invoice these services limits the development of academic Plastic Surgery. In 12 of 36 university hospitals exists no kind of Plastic Surgery. Moreover Plastic Surgery is no integral part in university education order defined by the medical approbation order (ÄApprO). However integration of Plastic Surgery with its interdisciplinary responsibility in the common university curriculum will be a forward looking appreciation of Plastic Surgery.}, author = {Kemper, Robert and Horch, Raymund E. and Giunta, Riccardo E. and Prantl, Lukas}, doi = {10.1055/a-0795-3583}, faupublication = {yes}, journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie}, keywords = {Academic Plastic Surgery; Diagnosis related groups; educational curriculum; German diagnosis-related groups; interdisciplinarity; University education}, note = {CRIS-Team Scopus Importer:2021-04-20}, pages = {144-150}, peerreviewed = {Yes}, title = {{Interdisziplinarität} in der akademischen {Plastischen} {Chirurgie} – belanglos oder zukunftsorientiert?}, volume = {51}, year = {2019} } @article{faucris.238438271, author = {Dragu, Adrian and Linke, Rainer and Kuwert, Torsten and Unglaub, Frank and Kneser, Ulrich and Stürzl, Michael and Horch, Raymund E. and Bach, Alexander D.}, doi = {10.1097/RLU.0b013e3181c3614d}, faupublication = {yes}, journal = {Clinical Nuclear Medicine}, month = {Jan}, pages = {36-7}, peerreviewed = {Yes}, title = {{Interesting} image. {Tc}-99m sestamibi {SPECT}/{CT} as a new tool for monitoring perfusion and viability of buried perforator based free flaps in breast reconstruction after breast cancer.}, volume = {35}, year = {2010} } @article{faucris.269956087, abstract = {Background: Assessment of tissue perfusion after irradiation of random pattern flaps still remains a challenge. Methods: Twenty-five rats received harvesting of bilateral random pattern fasciocutaneous flaps. Group 1 served as nonirradiated control group. The right flaps of the groups 2–5 were irradiated with 20 Gy postoperatively (group 2), 3 × 12 Gy postoperatively (group 3), 20 Gy preoperatively (group 4) and 3 × 12 Gy preoperatively (group 5). Imaging with infrared thermography, indocyanine green angiography and near-infrared reflectance-based imaging were performed to detect necrotic areas of the flaps. Results: Analysis of the percentage of the necrotic area of the irradiated flaps showed a statistically significant increase from day 1 to 14 only in group 5 (p < 0.05). Indocyanine green angiography showed no differences (p > 0.05) of the percentage of the nonperfused area between all days in group 1 and 3, but a decrease in group 2 in both the left and the right flaps. Infrared thermography and near-infrared reflectance-based imaging did not show evaluable differences. Conclusion: Indocyanine green angiography is more precise in prediction of necrotic areas in random pattern skin flaps when compared to hyperspectral imaging, thermography or clinical impression. Preoperative fractional irradiation with a lower individual dose but a higher total dose has a more negative impact on flap perfusion compared to higher single stage irradiation.}, author = {Müller-Seubert, Wibke and Ostermaier, Patrick and Horch, Raymund E. and Distel, Luitpold and Frey, Benjamin and Cai, Aijia and Arkudas, Andreas}, doi = {10.3390/jpm12020237}, faupublication = {yes}, journal = {Journal of Personalized Medicine}, keywords = {Imaging; Irradiation; Malperfusion}, note = {CRIS-Team Scopus Importer:2022-02-25}, peerreviewed = {Yes}, title = {{Intra}-and {Early} {Postoperative} {Evaluation} of {Malperfused} {Areas} in an {Irradiated} {Random} {Pattern} {Skin} {Flap} {Model} {Using} {Indocyanine} {Green} {Angiography} and {Near}-{Infrared} {Reflectance}-{Based} {Imaging} and {Infrared} {Thermography}}, volume = {12}, year = {2022} } @article{faucris.212706360, abstract = {Background: The introduction of the opportunity to transplant a viable uterus into women for fulfilling their desire to have a child has awakened high expectations worldwide. Materials and Methods: A sheep model was used to evaluate tools for optimizing measurement of blood flow in uterine transplantation. Intraoperatively, blood flow was measured using unidirectional Doppler and indocyanine green (ICG) fluorescence imaging. Postoperatively, an implantable Doppler probe served as a tool for clinical monitoring the patency of anastomosed vessels. Results: ICG imaging showed complete vascularization of the uterus before and in short-term evaluation after surgery. The implantable Doppler probe proved to be highly suitable for assessing patency of vessels in a non-invasive way. Results of histology, and real-time polymerase chain reaction demonstrated viability of the transplanted uterus. Conclusion: Different methods to monitor vasculature patency have proven to be advantageous in supporting both surgeons and researchers in ensuring successful implementation of uterine transplantation.}, author = {Kengelbach-Weigand, Annika and Lotz, Laura and Schmid, Rafael and Lang, Werner and Beckmann, Matthias and Hoffmann, Inge and Horch, Raymund E. and Renner, Stefan and Dittrich, Ralf and Boos, Anja and Hildebrandt, Thomas}, doi = {10.21873/invivo.11478}, faupublication = {yes}, journal = {In Vivo}, note = {CRIS-Team WoS Importer:2019-03-08}, pages = {325-336}, peerreviewed = {Yes}, title = {{Intra}- and {Postoperative} {Blood} {Flow} {Monitoring} in a {Sheep} {Model} of {Uterus} {Transplantation}}, volume = {33}, year = {2019} } @article{faucris.315837203, abstract = {Background: Arteriovenous (AV) loops help to overcome absent or poor-quality recipient vessels in highly complex microvascular free flap reconstruction cases. There are no studies on blood flow and perfusion patterns. The purpose of this study was to evaluate and compare intraoperative hemodynamic characteristics of AV loops followed by free tissue transfer for thoracic wall and lower extremity reconstruction. Methods: this prospective clinical study combined Transit-Time Flowmetry and microvascular Indocyanine Green Angiography for the assessment of blood flow volume, arterial vascular resistance and intrinsic transit time at the time of AV loop construction and on the day of free flap transfer. Results: A total of 11 patients underwent AV loop creation, of whom five required chest wall reconstruction and six required reconstruction of the lower extremities. In seven of these cases, the latissimus dorsi flap and in four cases the vertical rectus abdominis myocutaneous (VRAM) flap was used as a free flap. At the time of loop construction, the blood flow volume of AV loops was 466 ± 180 mL/min, which increased to 698 ± 464 mL/min on the day of free tissue transfer (p > 0.1). After free flap anastomosis, the blood flow volume significantly decreased to 18.5 ± 8.3 mL/min (p < 0.001). There was no significant difference in blood flow volume or arterial vascular resistance between latissimus dorsi and VRAM flaps, nor between thoracic wall and lower extremity reconstruction. However, a significant correlation between the flap weight and the blood flow volume, as well as to the arterial vascular resistance, was found (p < 0.05). Conclusion: This is the first study to perform intraoperative blood flow and hemodynamic measurements of AV loops followed by free tissue transfer. Our results show hemodynamic differences and contribute to deeper understanding of the properties of AV loops for free flap reconstruction.}, author = {Geierlehner, Alexander and Horch, Raymund E. and Ludolph, Ingo and Lang, Werner and Rother, Ulrich and Meyer, Alexander and Arkudas, Andreas}, doi = {10.3390/jcm12237477}, faupublication = {yes}, journal = {Journal of Clinical Medicine}, keywords = {ICG fluoroscopy; interdisciplinary plastic surgery; microsurgery; plastic surgery; reconstructive surgery; surgical innovation}, note = {CRIS-Team Scopus Importer:2023-12-22}, peerreviewed = {Yes}, title = {{Intraoperative} {Blood} {Flow} {Analysis} of {Free} {Flaps} with {Arteriovenous} {Loops} for {Autologous} {Microsurgical} {Reconstruction}}, volume = {12}, year = {2023} } @article{faucris.111568644, abstract = {In this report, we present a case of the use of a conjoined fabricated free anterolateral thigh (ALT)/tensor fascia latae (TFL) perforator flap for reconstruction of the lower extremity with intraoperative flap design using intraoperative indocyanine green (ICG) monitoring. The flap was used for reconstruction of a 16 cm × 28 cm sized defect of the lower leg in a 24-year-old man. The defect was caused by a third degree open fracture to the tibia. Upon dissection of the ALT perforators, ICG monitoring showed that both dominant ALT perforators did not yield a sufficient perforasome (~16 cm × 17 cm) for the larger flap needed. An adjacent TFL perforator also supplied a large perforasome (~15 cm × 11 cm), so a conjoined fabricated flap was harvested and transplanted to cover an extensive lower leg defect. The artery of the TFL perforator pedicle was being in-flap anastomosed to a side branch of the ALT pedicle. Postoperative course was uneventful and there were no complications. Length of follow-up was 6 months, aesthetic and functional outcome was good. The patient was very satisfied with the aesthetic outcome. Both legs were fully mobile after intensive physiotherapy for the reconstructed leg. The leg where the flap had been harvested showed full strength in knee joint flexion. This case could show that identification of the supplying vessels may be possible by ICG monitoring. © 2015 Wiley Periodicals, Inc. Microsurgery 36:684-688, 2016.}, author = {Bührer, Gregor and Taeger, Christian and Ludolph, Ingo and Horch, Raymund E. and Beier, Justus}, doi = {10.1002/micr.22424}, faupublication = {yes}, journal = {Microsurgery}, note = {EVALuna2:15607}, pages = {684-688}, peerreviewed = {Yes}, title = {{Intraoperative} flap design using {ICG} monitoring of a conjoined fabricated anterolateral thigh/tensor fasciae latae perforator flap in a case of extensive soft tissue reconstruction at the lower extremity}, volume = {36}, year = {2016} } @article{faucris.217808954, abstract = {The surgically induced angiogenesis by means of arteriovenous (AV) loops represents a powerful method to significantly enhance vascularization of biomaterials. Regarding tissue engineering applications, spider silk is a promising biomaterial with a good biocompatibility and slow biodegradation. This study aims at investigating vascularization as well as de novo tissue formation of fibrous matrices made of electro-spun (ES) or wet-spun (WS) engineered ADF4(C16) spider silks in the rat AV loop model. Either ES or WS spider silk fibrous matrices were filled into Teflon chambers. Intrinsic vascularization was induced by means of an AV loop. After 4 weeks of vascularization, de novo tissue formation and biocompatibility were analyzed. Regardless of their significantly differing fiber diameters, both ES and WS eADF4(C16) fiber matrices displayed a good biocompatibility and initiated de novo tissue formation as well as vessel formation. Both matrices demonstrated partial vascularization originating from the AV loop, with more vessels in spider silk matrices with lower fiber diameters. We were able to demonstrate intrinsic vascularization of spider silk fibrous matrices by means of the AV loop. Moreover, our study indicates that the adjustment of the fiber diameter of engineered spider silks enables new possibilities to optimize vascularization.}, author = {Steiner, Dominik and Lang, Gregor and Fischer, Laura and Winkler, Sophie and Fey, Tobias and Greil, Peter and Scheibel, Thomas and Horch, Raymund E. and Arkudas, Andreas}, doi = {10.1089/ten.tea.2018.0360}, faupublication = {yes}, journal = {Tissue Engineering: Parts A, B, and C}, note = {CRIS-Team WoS Importer:2019-05-17}, peerreviewed = {unknown}, title = {{Intrinsic} {Vascularization} of {Recombinant} {eADF4}({C16}) {Spider} {Silk} {Matrices} in the {Arteriovenous} {Loop} {Model}}, year = {2019} } @article{faucris.210308767, abstract = {The application of tissue engineered constructs is an approach in regenerative therapies to support regeneration of damaged muscular tissue. Therefore the development of highly aligned electrospun scaffolds based on polycaprolactone (PCL) and collagen enables a versatile development towards tailor made applications. However, the application of natural polymers like collagen brings the risk of batch-to-batch inconsistencies, which influence the reproducibility of the electrospinning process. Aligned PCL-Collagen nanofibers were fabricated via electrospinning using benign solvents. The spinnability of different collagen batches and polymer concentrations in diluted acetic acid as solvent was investigated. Furthermore spinning parameters and fiber morphology were investigated in order to determine the most stable spinning conditions and analyze the batch-to-batch variations. Finally the effect of the solution temperature and the time of pure collagen in solution were investigated, to complete the analysis of the influences on the spinning behavior.}, author = {Dippold, Dirk and Hardt, Moritz and Boccaccini, Aldo R. and Horch, Raymund E. and Beier, Justus P. and Schubert, Dirk W. and Cai, Aijia}, doi = {10.1016/j.msec.2018.10.057}, faupublication = {yes}, journal = {Materials Science and Engineering C}, pages = {217-225}, peerreviewed = {Yes}, title = {{Investigation} of the batch-to-batch inconsistencies of {Collagen} in {PCL}-{Collagen} nanofibers.}, volume = {95}, year = {2019} } @article{faucris.119796424, abstract = {In addition to good mechanical properties needed for three-dimensional tissue engineering, the combination of alginate dialdehyde, gelatin and nano-scaled bioactive glass (45S5) is supposed to combine excellent cellular adhesion, proliferation and differentiation properties, good biocompatibility and predictable degradation rates. The goal of this study was to evaluate the in vitro and in vivo biocompatibility as a first step on the way to its use as a scaffold in bone tissue engineering. In vitro evaluation showed good cell adherence and proliferation of bone marrow derived mesenchymal stem cells seeded on covalently crosslinked alginate dialdehyde-gelatin (ADA-GEL) hydrogel films with and without 0.1% nano-Bioglass® (nBG). Lactate dehydrogenase (LDH)- and mitochondrial activity significantly increased in both ADA-GEL and ADA-GEL-nBG groups compared to alginate. However, addition of 0.1% nBG seemed to have slight cytotoxic effect compared to ADA-GEL. In vivo implantation did not produce a significant inflammatory reaction, and ongoing degradation could be seen after four weeks. Ongoing vascularization was detected after four weeks. The good biocompatibility encourages future studies using ADA-GEL and nBG for bone tissue engineering application. © 2014 by the authors.}, author = {Rottensteiner, Ulrike and Sarker, Bapi and Heusinger, Dominik and Dafinova, Diana and Rath, Subha N. and Beier, Justus and Kneser, Ulrich and Horch, Raymund E. and Detsch, Rainer and Boccaccini, Aldo R. and Arkudas, Andreas}, doi = {10.3390/ma7031957}, faupublication = {yes}, journal = {Materials}, keywords = {Alginate dialdehyde; Biocompatibility; Gelatin; Mesenchymal stem cells; Nano-Bioglass®; Tissue engineering}, pages = {1957-1974}, peerreviewed = {Yes}, title = {{In} vitro and in vivo biocompatibility of alginate dialdehyde/gelatin hydrogels with and without nanoscaled bioactive glass for bone tissue engineering applications}, volume = {7}, year = {2014} } @article{faucris.108944484, abstract = {Abstract Cobalt ions are known to stimulate angiogenesis via inducing hypoxic conditions and hence are interesting agents to be used in conjunction with bioactive glasses (BGs) in bone tissue engineering approaches. In this work we investigated in vitro cell biocompatibility of Co releasing 1393 BG composition (in wt.%: 53SiO
This experiment is comprised of four steps: harvesting of the flexor tendons from fresh cadaveric hands, transection of the tendons in a standardized manner, tendon repair by four different techniques, mounting, and measurement of the tendon repairs on a standard linear dynamometer. The UHMWPE and PTFE showed comparable mechanical properties and were significantly superior to PPL in terms of linear traction strength. Repairs with four- and six-strand techniques proved stronger than two-strand techniques. Handling and knotting of PTFE are a challenge due to very low surface friction but fastening of the four- or six-strand repair is comparatively easy to achieve. Surgeons routinely use PTFE suture material in cardiovascular surgery and breast surgery. The PTFE strands are suitable for use in tendon surgery, providing a robust tendon repair so that early active motion regimens for rehabilitation can be applie}, author = {Polykandriotis, Elias and Himmler, Marcus and Mansouri, Shirin and Ruppe, Florian and Grüner, Jasmin and Bräuer, Lars and Schubert, Dirk W. and Horch, Raymund E.}, doi = {10.3791/64115-v}, faupublication = {yes}, keywords = {Polytetrafluoroethylene; PTFE; Suture Material; Tendon Surgery; Mechanical Properties; Flexor Tendon Repair; Linear Tension Strength; Cadaveric Upper Limb; Incision; A1 Pulley; Flexor Digitorum Profundus; Tendon Retractor; Traction}, peerreviewed = {unknown}, title = {{JoVE} {Video} {Dataset}}, year = {2022} } @article{faucris.226665004, abstract = {BACKGROUND Skin replacement by means of cultured epithelial keratinocytes is a well-accepted method. However, several clinical drawbacks of sheet autografts (CEA - cultured epithelial autografts) have stimulated various efforts to optimize cell culture and cell delivery. Recent developments include use of cell monolayers instead of a fully differentiated epithelium, as well as use of various biomaterials to grow and transport the cultured cells. To optimize the transfer of human keratinocytes directly to the recipient wound bed, we used an "upside-down" technique, delivering cultured cells directly to the wound with the carrier material on top. MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone. RESULTS In the upside-down group, 14 days after grafting, a multi-layered, differentiating epidermis was found, whereas the wounds in the upside-up group and in the control group were not completely closed up to day 21. Persistence of human keratinocytes was shown in the upside-down group only, from day 7 until day 35 after grafting. CONCLUSIONS This study confirms that upside-down grafting of subconfluent monolayers of serum-free cultured human keratinocytes on esterified hyaluronic acid membranes is a suitable means to transfer actively proliferative keratinocytes, and reduces wound contraction. Compared to standard grafting protocols of cultured epithelium, such as CEA sheet grafts, it is easier to apply, does not need enzymatic detachment of cells from the culture dish, and limits the number of production steps required.}, author = {Horch, Raymund E. and Wagner, Gilbert and Bannasch, Holger and Kengelbach-Weigand, Annika and Arkudas, Andreas and Schmitz, Marweh}, doi = {10.12659/MSM.915649}, faupublication = {yes}, journal = {Medical Science Monitor}, note = {CRIS-Team Scopus Importer:2019-09-17}, pages = {6702-6710}, peerreviewed = {Yes}, title = {{Keratinocyte} {Monolayers} on {Hyaluronic} {Acid} {Membranes} as "{Upside}-{Down}" {Grafts} {Reconstitute} {Full}-{Thickness} {Wounds}}, volume = {25}, year = {2019} } @article{faucris.246688022, author = {Horch, Raymund E.}, doi = {10.1055/a-0897-4061}, faupublication = {yes}, journal = {Zentralblatt für Chirurgie}, note = {CRIS-Team Scopus Importer:2020-12-11}, pages = {519-520}, peerreviewed = {No}, title = {{Kommentar} zur {Arbeit}: {Myostatin} als potenzieller {Marker} für das {Kompartmentsyndrom} bei elektrischen {Verletzungen}}, volume = {145}, year = {2020} } @article{faucris.264059780, abstract = {Background: There is still no consensus regarding the ideal zoning in abdominal-based autologous breast reconstruction using free DIEP or ms-TRAM flaps. In particular, the perfusion pattern of the flap according to the number of perforators used and their location remains controversial. In this study, the perfusion of free DIEP and ms-TRAM flaps is assessed intraoperatively and analyzed with regard to different perfusion patterns. Methods: A retrospective analysis of 100 free flaps for breast reconstruction was performed. Following complete flap harvest, we used indocyanine green angiography for perfusion analysis. By applying two different contour levels, DIEP flaps with lateral or medial perforators and ms-TRAM flaps were assessed for their respective perfusion patterns. Results: No statistically significant differences were found in the size of the perfusion area between the different flap types when applying the contour level of 20% (p >0.05). For the contour level of 30%, however, statistically significant differences were found between DIEP flaps with medially or laterally located perforators (p = 0.038). Laterally or medially located perforators in DIEP flaps showed no significant differences in their ability to cross the midline (contour level 20%, p = 0.068; contour level 30%, p = 0.058). Conclusion: Considering the variability of the perfusion of the abdominal wall and the high sensitivity of indocyanine green angiography for their detection, the abdominal zonings play a minor role. By using intraoperative indocyanine green angiography, a precise and patient-specific free flap surgery for autologous breast reconstruction is possible independent of perforator location.}, author = {Ludolph, Ingo and Bettray, Dominik and Beier, Justus and Horch, Raymund E. and Arkudas, Andreas}, doi = {10.1016/j.bjps.2021.08.002}, faupublication = {yes}, journal = {Journal of Plastic Reconstructive and Aesthetic Surgery}, keywords = {Breast reconstruction; DIEP; ICG; Indocyanine green; Perfusion; Zone}, note = {CRIS-Team Scopus Importer:2021-09-17}, peerreviewed = {Yes}, title = {{Leaving} the perfusion zones? {Individualized} flap design in 100 free {DIEP} and ms-{TRAM} flaps for autologous breast reconstruction using indocyanine green angiography}, year = {2021} } @article{faucris.255673010, abstract = {Background: The muscle-sparing latissimus dorsi flap poses an alternative to the conventional latissimus dorsi flap when a defect requires a smaller flap volume with a long vascular pedicle. The aim of the study was to analyze the functional outcome following muscle-sparing versus conventional harvest of a latissimus dorsi flap. Material and Methods: Patients who received a muscle-sparing latissimus dorsi muscle flap transplantation (group 1) and patients with a complete latissimus dorsi muscle flap harvest (group 2) for defect reconstruction at the University Hospital of Erlangen between 2007 and 2016 were examined. The evaluation included a physical examination with the measurement of strength and range of motion of the shoulder of both the donor side as well as the nonoperated side. Furthermore, the DASH score was evaluated. Additionally, patients in group 1 received a neurophysiological examination. Results: The DASH score did not show statistically significant differences between both groups. Both strength (p = 0.031) and range of motion (p<0.05) of the shoulder of the donor side were statistically significantly lower than the nonoperated side in group 2, while no difference was found in group 1. The neurophysiological examination in group 1 showed a reduced nerve conduction velocity of the donor side as compared to the nonoperated side in most patients (60% and n = 3). Conclusion: Harvesting the muscle-sparing latissimus dorsi flap leads to less functional impairments of the shoulder than harvesting the complete latissimus dorsi flap.}, author = {Müller-Seubert, Wibke and Scheibl, Karsten and Bührer, Gregor and Möbius, Cornelia and Ludolph, Ingo and Horch, Raymund E. and Arkudas, Andreas}, doi = {10.1016/j.bjps.2021.02.007}, faupublication = {yes}, journal = {Journal of Plastic Reconstructive and Aesthetic Surgery}, keywords = {Defect reconstruction; Functional impairment; Latissimus dorsi flap; Muscle sparing latissimus dorsi flap}, note = {CRIS-Team Scopus Importer:2021-04-19}, peerreviewed = {Yes}, title = {{Less} is more – retrospective comparison of shoulder strength and range of motion between conventional and muscle-sparing harvesting technique of a latissimus dorsi flap}, year = {2021} } @article{faucris.240047111, abstract = {The purpose of this study was to analyse the outcome of our established triple treatment strategy in therapy-resistant deep-thickness chronic lower leg ulcers. This limb salvage approach consists of ultra-radical surgical debridement, negative-pressure wound therapy (NPWT) with or without instillation, and split-thickness skin grafting. Between March 2003 and December 2019, a total of 16 patients and 24 severe cases of lower leg ulcers were eligible for inclusion in this highly selective population. A total of seven patients received immunosuppressive medication. Complete wound closure was achieved in 25% and almost 90% of included lower leg ulcer cases after 3 and 24 months of our triple treatment strategy, respectively. The overall limb salvage rate was 100%. Bacterial colonisation of these wounds was significantly reduced after multiple surgical debridements and NPWT. Fasciotomy and radical removal of devitalised tissue such as deep fascia, tendons, and muscles combined with NPWT showed promising results in terms of the overall graft take rate. This treatment strategy was considered as last resort for limb salvage in such a critically ill and immunocompromised patient population. Surgeons should be aware of its efficacy and consider the triple treatment strategy especially if no other limb salvage option remains.}, author = {Geierlehner, Alexander and Horch, Raymund E. and Müller-Seubert, Wibke and Arkudas, Andreas and Ludolph, Ingo}, doi = {10.1111/iwj.13428}, faupublication = {yes}, journal = {International Wound Journal}, keywords = {chronic leg ulcer; negative-pressure wound therapy; skin transplantation; ultra-radical debridement}, note = {CRIS-Team Scopus Importer:2020-07-03}, peerreviewed = {Yes}, title = {{Limb} salvage procedure in immunocompromised patients with therapy-resistant leg ulcers-{The} value of ultra-radical debridement and instillation negative-pressure wound therapy}, year = {2020} } @article{faucris.256886811, abstract = {Atrophy of tongue after free-flap reconstruction often leads to functional problems like dysphagia and articulation disorders. Lipotransfer might resolve this issue. We describe a case of repeated lipotransfer into an atrophied reconstructed tongue. A 66-year-old patient with a squamous cell carcinoma of the tongue underwent hemiglossectomy and reconstruction of the tongue by a free radial forearm flap. After atrophy of the reconstructed tissue, repeated sessions of lipotransfer were undertaken to augment the atrophic tissue. Three sessions of lipotransfer resulted in satisfactory augmentation of the atrophied reconstructed tongue, leading to improvement of dysphagia and articulation. To our knowledge, this is the first report of lipotransfer used to augment a flap inside the oral cavity. We propose this tool as a potential aid to improve typical tongue reconstruction sequelae.}, author = {Cai, Aijia and Iro, Heinrich and Horch, Raymund E.}, doi = {10.1055/a-1098-8665}, faupublication = {yes}, journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie}, keywords = {Lipotransfer; Neozunge; Schluckstörung; Sprechstörung; Zungenrekonstruktion}, note = {CRIS-Team Scopus Importer:2021-04-30}, pages = {201-204}, peerreviewed = {Yes}, title = {{Lipotransfer} führt zu {Verbesserung} von {Schluck}- und {Artikulationsstörungen} nach mikrochirurgischer {Zungenrekonstruktion} – ein {Fallbericht}}, volume = {53}, year = {2021} } @article{faucris.221130691, abstract = {Acellular dermal matrices have recently increasingly been used in alloplastic breast reconstruction with silicone breast implants. Among these matrices, acellular porcine dermis (APD) is frequently applied, but long-term data on tissue integration and capsular fibrosis formation are still missing. Silicone prostheses with (group A) and without (group B) APD as an implant-covering shell were implanted in male Lewis rats. At 3, 12, and 52 weeks after implantation, the constructs were explanted. Molecular biological and immunohistochemical analyses were performed afterwards. On comparing the collagenous layer and the newly formed myofibroblast-rich layer around the implants of both groups, it became apparent that in group A, these layers were thinner, followed by a lower expression of TGFβ1 after 12 and 52 weeks. Further, in this group, at the endpoint of 52 weeks, a lower amount of CD68-positive cells in the collagenous and myofibroblast-rich layers were observed and the expression of TNFα was reduced, while the number of Ki67-positive cells was significantly higher with time. Furthermore, MMP1 expression in group A was lower than that in group B, and the calculated ratio of MMP1:TIMP1 expression was higher. The long-term results clearly show a reduction in inflammatory and fibrotic tissue reaction when APD is used to cover silicone prostheses. These experimental data will be of considerable importance for implant-based breast surgery, as they indicate a potential benefit in the reduction of capsular fibrosis formation of an interposition of APD between the recipient and the silicone implant.}, author = {Ludolph, I. and Grüner, Johanna and Kengelbach-Weigand, Annika and Fießler, Cornelia and Horch, Raymund E. and Schmitz, Marweh}, doi = {10.1016/j.bjps.2019.04.015}, faupublication = {yes}, journal = {Journal of Plastic Reconstructive and Aesthetic Surgery}, keywords = {Acellular porcine; Breast reconstruction; Capsular contracture; Capsular fibrosis; Dermal matrix}, note = {CRIS-Team Scopus Importer:2019-06-21}, peerreviewed = {Yes}, title = {{Long}-term studies on the integration of acellular porcine dermis as an implant shell and the effect on capsular fibrosis around silicone implants in a rat model}, year = {2019} } @article{faucris.244850776, abstract = {Purpose: Symptomatic macromastia causes physical and psychological problems that can lead to restrictions in the patients’ social and working lives and a reduced quality of life. Associated medical treatments also have a considerable impact on health-care costs. Several studies have assessed these costs, but the total disease costs of macromastia have never been evaluated on the basis of real-world data. Methods: The data for 76 patients who underwent reduction mammoplasty between 2008 and 2016 were collected using a two-part questionnaire (preoperative and postoperative), as well as the patient files. Topics surveyed, besides demographic data, included physician visits, medical imaging, medical procedures, medical treatments, rehabilitation and convalescent measures, drug intake, medical aids, exercise activity, and sick leave days before surgery, to calculate the costs per year of conservative treatment of symptomatic macromastia. Results: The mean time from start of symptoms to surgery was 11.82 years. The data for this group of patients with symptomatic macromastia show that costs per patient amount to €1677.55 per year. These costs include medical consultation, radiological imaging, medical treatments and procedures, physical therapy and rehabilitation, medication, special brassieres, exercise classes costs for sick leave due to problems with macromastia, and travel expenses. Conclusions: These results show that considerable health-care costs arise due to macromastia with conservative treatment. Overall, macromastia costs €1677.55 per patient/year. In particular, lost productivity due to sick days and the costs of physiotherapy are factors driving the high costs.}, author = {Jud, Sebastian and Brendle-Behnisch, Anne and Hack, Carolin and Preuss, Caroline and Arkudas, Andreas and Horch, Raymund E. and Beckmann, Matthias and Lux, Michael P.}, doi = {10.1007/s00404-020-05841-7}, faupublication = {yes}, journal = {Archives of Gynecology and Obstetrics}, keywords = {Breast reduction mammoplasty; Conservative treatment; Disease costs; Gigantomastia; Health care; Macromastia}, note = {CRIS-Team Scopus Importer:2020-11-06}, peerreviewed = {Yes}, title = {{Macromastia}: an economic burden? {A} disease cost analysis based on real-world data in {Germany}}, year = {2020} } @article{faucris.120171744, abstract = {The importance of Mg in the human body, its key role in bone tissue development, in addition to its application to improve and modify physical, thermal, and mechanical properties of bioactive silicate glasses, make Mg a very interesting element as a component of bioactive glasses for medical applications. Although Mg is a typical element in the composition of numerous developed bioactive glasses, the analysis of the literature reveals that further research is required to gain comprehensive understanding about the effects of MgO on bioactive glass structure and properties. This article presents a comprehensive overview of the field of Mg-containing bioactive glasses discussing available compositions and summarizing existing knowledge on effects of MgO on glass properties. The biomedical applications of several developed glasses are discussed highlighting the distinct effects of Mg in relevant areas, such as bioactivity and cell response, and focusing on the most common applications for these glasses, such as bone cements, bioactive coatings, and scaffolds for bone tissue engineering. The effect of Mg on bone development is discussed and avenues for future research in the field are proposed, emphasizing the need to investigate unstudied properties of (already developed) Mg-containing glass compositions, such as angiogenesis-stimulating action and the effect on osteoclast functions, to develop new Mg-containing bioactive glasses as well as to make products of different shape designs such as nanoparticles, fibers, and complex porous structures. © 2012 The American Ceramic Society and Wiley Periodicals, Inc.}, author = {Diba, Mani and Tapia, Felipe and Boccaccini, Aldo R. and Strobel, Leonie}, doi = {10.1111/j.2041-1294.2012.00095.x}, faupublication = {yes}, journal = {International Journal of Applied Glass Science}, note = {UnivIS-Import:2015-03-09:Pub.2012.tech.IW.LM.magnes}, pages = {221-253}, peerreviewed = {Yes}, title = {{Magnesium}-{Containing} {Bioactive} {Glasses} for {Biomedical} {Applications}}, volume = {3}, year = {2012} } @article{faucris.237246041, abstract = {BACKGROUND: During the past decades, the use of vascular closure devices (VCDs) has been established due to the increasing number of interventions. Of particular concern is the perceived risk of VCDs for development of implant-induced vascular major complications. Therefore, the aim of this analysis was to report the variety of vascular access side complications after the use of VCDs, and to analyze the referred risk factors. METHODS: All cases of vascular complications associated with VCD use requiring surgical repair from 2010 to 2016 were retrospectively analyzed in a single center trial. Systemic and local complications, risk factors as pre-existing diseases and their influence on the surgical outcome were evaluated. RESULTS: A total of 46 individuals were included in this study (male/female: 16/30). The total number of interventions in the involved departments of the study center during the same period was 9754 Therefore the complication rate after VCD implantation was 0.47%. The detected complications ranged from symptomatic claudication (N.=24) to acute leg ischemia (N.=19) and major bleeding (N.=3). Surgical reconstruction was performed by direct suture (N.=4), transverse arteriotomy with thrombectomy (N.=2), endarterectomy with patchplasty (N.=35), inguinal graft interposition or bypass (N.=3) and primary major amputation (N.=2). In regard to the risk factors, the study has concluded that atherosclerosis, female gender and diabetes mellitus are correlated with major vascular complications (bleeding and limb ischemia). However, in cases with therapeutic anticoagulation the rate of major bleeding was significantly elevated (P=0.028). CONCLUSIONS: Reported complication rates associated with femoral VCD implantation are low. However, in some cases VCD implantation may account for severe complications including limb losses. Therefore, an adequate patient selection is necessary to detect those cases at higher risk for complications.}, author = {Chatzigeorgiadis, Pavlos and Hellwig, Konstantin and Almási-Sperling, Veronika and Meyer, Alexander and Lang, Werner and Rother, Ulrich}, doi = {10.23736/S0392-9590.19.04253-6}, faupublication = {yes}, journal = {International Angiology}, note = {CRIS-Team Scopus Importer:2020-04-14}, pages = {139-144}, peerreviewed = {Yes}, title = {{Major} vascular complications after transfemoral arterial closure system implantation: a single-center study}, volume = {39}, year = {2020} } @article{faucris.247768609, abstract = {BACKGROUND: A promising and useful development of negative-pressure wound therapy (NPWT) is the addition of instillation and dwell time of topical wound solutions (NPWTi-d). Uses of NPWTi-d include acute and traumatic wounds, whereby wound closure may be facilitated via wound cleansing and promotion of granulation tissue formation. This systematic review summarizes publications on NPWTi-d in the treatment of acute and traumatic wounds. METHODS: A systematic review was performed analyzing articles from major clinical databases. Only clinical studies ≥10 patients reporting on the application of NPWTi-d in acute and traumatic wounds were included. RESULTS: One hundred ninety-two articles were retrieved, of which 10 articles met inclusion criteria. Of those, 2 were lesser-quality randomized controlled trials, comparative studies or prospective cohorts, 2 were retrospective studies, and 6 retrospective cohort studies. In total, included publications reported 109 patients with acute and traumatic wounds treated with NPWTi-d. Data from these studies indicated the potential for reduction in bacterial bioburden through wound cleansing and promotion of granulation tissue formation, thereby facilitating wound closure, reduced length of therapy and hospital time. However, for most publications, different wound causes and subsequently no isolated results for acute and traumatic wounds were reported. CONCLUSION: NPWTi-d has promise to be effective in facilitating wound closure and reducing the time for wound closure. The present systematic review demonstrates a relatively low level of evidence available to objectively support this effect. To underline these positive results, large prospective, randomized controlled trials are necessary to manifest the role of NPWTi-d in the daily clinical routine for this wound category.}, author = {Diehm, Yannick F. and Fischer, Sebastian and Wirth, Garrett A. and Haug, Vanessa and Orgill, Dennis P. and Momeni, Arash and Horch, Raymund E. and Lehner, Burkhard and Kneser, Ulrich and Hirche, Christoph}, doi = {10.1097/PRS.0000000000007610}, faupublication = {yes}, journal = {Plastic and Reconstructive Surgery}, month = {Jan}, note = {CRIS-Team Scopus Importer:2021-01-15}, pages = {43S-53S}, peerreviewed = {Yes}, title = {{Management} of {Acute} and {Traumatic} {Wounds} {With} {Negative}-{Pressure} {Wound} {Therapy} {With} {Instillation} and {Dwell} {Time}}, volume = {147}, year = {2021} } @article{faucris.109443444, abstract = {We report the case of an 86-year-old man with severe wound infection originating from a chronic crural ulcer of the lower limb, which under negative pressure wound therapy led to excessive tissue necrosis and perforation of the anterior tibial artery. A swab taken 10 and 7 days preoperatively was positive for Helcococcus kunzii. H. kunzii has been described as a potentially pathogenic organism. The questions whether the negative pressure wound therapy itself caused the bleeding or the negative pressure wound therapy, which generates an anaerobic atmosphere, has triggered the growth and invasion of the facultative anaerobic bacterium H. kunzii and owing to the infection the artery perforated or whether the bacteria has no influence at all remain currently unanswered. After surgical debridement the signs of infection were completely eliminated, and a free musculocutaneous flap led to rapid healing of the wound. Following which H. kunzii was no longer detectable.}, author = {Stanger, Katrin M. and Albert, Frauke and Kneser, Ulrich and Bogdan, Christian and Horch, Raymund E.}, doi = {10.1111/iwj.12133}, faupublication = {yes}, journal = {International Wound Journal}, note = {EVALuna2:7649}, pages = {443-6}, peerreviewed = {Yes}, title = {{Management} of chronic osteomyelitis of the tibia with life-threatening complications under negative pressure wound therapy and isolation of {Helcococcus} kunzii}, volume = {12}, year = {2015} } @article{faucris.204383534, abstract = {The introduction of neoadjuvant radiotherapy has allowed limb-preserving surgical treatment in patients with extremity soft tissue sarcoma, and the overall prognosis of this approach is similar to that of limb amputation. The benefits of this treatment, however, are often accompanied with a higher risk of major complications and blood vessel damage because of radiation-induced inflammation and necrosis of the vessel wall. In particular, it is associated with the rupture of large vessels like the femoral artery and more severe complications of wounds located in the proximal lower extremity. We present a series of four patients with soft tissue sarcoma of the thigh undergoing tumour removal and reconstruction of the defect after neoadjuvant radiotherapy. The post-operative outcome depended on the total dose of radiation used as well as the closing technique of the resulting wound. Major wound complications occurred when the irradiated skin was closed directly over the resection cavity or when local skin flaps were used. In one case, severe radiation-induced life-threatening multiple bleeding events occurred, which led to multiple flap failures. Even if the skin cover is not a problem, we propose a combined interdisciplinary approach involving immediate plastic surgical transfer of healthy, well-vascularised tissue into a defect resulting from oncological resection of sarcoma of the lower extremity following neoadjuvant radiotherapy in order to avoid secondary wound breakdown and severe bleeding complications.}, author = {Cai, Aijia and Boos, Anja and Arkudas, Andreas and Horch, Raymund E.}, doi = {10.1111/iwj.12681}, faupublication = {yes}, journal = {International Wound Journal}, note = {EVALuna2:32842}, pages = {708-715}, peerreviewed = {Yes}, title = {{Management} of extremely hard-to-heal extremity wounds with severe life-threatening complications}, volume = {14}, year = {2017} } @inproceedings{faucris.120199244, author = {Daenicke, Jonas and Horch, Raymund E. and Schubert, Dirk W.}, booktitle = {Abstracts}, date = {2012-09-12/2012-09-14}, faupublication = {yes}, peerreviewed = {unknown}, title = {{Mapping} of the {Mechanical} {Properties} of {Silicone} {Breast} {Implants}}, venue = {Halle}, year = {2012} } @article{faucris.124006784, author = {Neeb, Karl-Hermann and Hilgert, Johannes}, doi = {10.1007/BF02573517}, faupublication = {no}, journal = {Semigroup Forum}, pages = {205--222}, peerreviewed = {Yes}, title = {{Maximality} of compression semigroups}, volume = {50}, year = {1995} } @article{faucris.111070344, abstract = {Merkel cell carcinoma is a rare tumor, which is associated with poor prognosis. It has been shown that radical local excision and radiotherapy are essentiell for curative therapy. To avoid compromise in tumor treatment, plastic surgery is often needed. Furthermore, using complex reconstruction methods, like free flap transplantation, has proven to be effective to avoid long and complicated clinical courses.Between 2003 and 2016 15 patients with Merkel cell carcinoma were treated in our department. In 2 cases additional resection had to take place before reconstruction was performed. In most cases, a safety margin of 2 cm was chosen. Complete excision could be achieved in 13 patients. We used different methods of reconstruction such as primary suturing, skin transplantations, local, pedicled or free flaps.In all patients defects could be reconstructed with good results, which proved to be stable even under postoperative radiation therapy.Given the modern techniques of plastic surgery the extent of local excision should not be should be chosen as radical as possible and necessary. It has been proven that interdisciplinary treatment of patients with Merkel cell carcinoma increases the chances for curative therapy while providing safe methods for reconstruction despite radiotherapy, extensive tumors or difficult localization.}, author = {Bührer, Gregor and Horch, Raymund E. and Schlabrakowski, Anne and Arkudas, Andreas}, doi = {10.1055/s-0042-104656}, faupublication = {yes}, journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie}, note = {EVALuna2:6765}, pages = {95-100}, peerreviewed = {Yes}, title = {{Merkel} {Cell} {Carcinoma} - {A} {Rare} {Entity} with {Specific} {Reconstructive} {Challenges}}, volume = {48}, year = {2016} } @article{faucris.111835284, author = {Witt, Ramona and Weigand, Annika and Boos, Anja and Dippold, Dirk and Schubert, Dirk W. and Boccaccini, Aldo R. and Arkudas, Andreas and Horch, Raymund E. and Beier, Justus}, doi = {10.1186/s12860-017-0131-2}, faupublication = {yes}, journal = {Bmc Cell Biology}, keywords = {HGF; IGF-1; Mesenchymal stem cells; Myogenic differentiation; PCL-collagen nanofibers; Skeletal muscle tissue engineering}, peerreviewed = {Yes}, title = {{Mesenchymal} stem cells and myoblast differentiation under {HGF} and {IGF}-1 stimulation for {3D} skeletal muscle tissue engineering}, volume = {18}, year = {2017} } @article{faucris.215837969, author = {Witt, Ramona and Weigand, Annika and Boos, Anja and Dippold, Dirk and Boccaccini, Aldo R. and Schubert, Dirk W. and Hardt, Moritz and Lange, Claudia and Arkudas, Andreas and Horch, Raymund E. and Beier, Justus and Cai, Aijia}, doi = {10.1186/s12860-017-0131-2}, faupublication = {yes}, journal = {Bmc Cell Biology}, keywords = {Mesenchymal stem cells; Myogenic differentiation; HGF; IGF-1; PCL-collagen nanofibers; Skeletal muscle tissue engineering}, peerreviewed = {Yes}, title = {{Mesenchymal} stem cells and myoblast differentiation under {HGF} and {IGF}-1 stimulation for {3D} skeletal muscle tissue engineering}, volume = {18}, year = {2017} } @article{faucris.234121312, abstract = {Lymphatic metastasis is one of the main prognostic factors concerning long-term survival of cancer patients. In this regard, the molecular mechanisms of lymphangiogenesis are still rarely explored. Also, the interactions between stem cells and lymphatic endothelial cells (LEC) in humans have not been well examined. Therefore, the main objective of this study was to assess the interactions between mesenchymal stem cells (MSC) and LEC using in vitro angiogenesis assays. Juvenile LEC were stimulated with VEGF-C, bFGF, MSC-conditioned medium (MSC-CM) or by co-culture with MSC. LEC proliferation was assessed using a MTT assay. Migration of the cells was determined with a wound healing assay and a transmigration assay. To measure the formation of lymphatic sprouts, LEC spheroids were embedded in collagen or fibrin gels. The LEC's capacity to form capillary-like structures was assessed by a tube formation assay on Matrigel® . The proliferation, migration and tube formation of LEC could be significantly enhanced by MSC-CM and by co-culture with MSC. The effect of stimulation with MSC-CM was stronger compared to stimulation with the growth factors VEGF-C and bFGF in proliferation and transmigration assays. Sprouting was stimulated by VEGF-C, bFGF and by MSC-CM. With this study, we demonstrate the potent stimulating effect of the MSC secretome on proliferation, migration and tube formation of LEC. This indicates an important role of MSC in lymphangiogenesis in pathological as well as physiological processes.}, author = {Robering, Jan Willem and Weigand, Annika and Pfuhlmann, Romy and Horch, Raymund E. and Beier, Justus and Boos, Anja}, doi = {10.1111/jcmm.13590}, faupublication = {yes}, journal = {Journal of Cellular and Molecular Medicine}, note = {EVALuna2:211472}, pages = {3740-3750}, peerreviewed = {Yes}, title = {{Mesenchymal} stem cells promote lymphangiogenic properties of lymphatic endothelial cells}, volume = {22}, year = {2018} } @article{faucris.111177264, abstract = {Intact microperfusion of the foot in the critical ischemic limb remains a crucial condition for ulcer healing. Aim of this clinical study was to evaluate the immediate effect of tibial bypass surgery on the microcirculation by usage of fluorescence angiography.Prospective analysis of 33 patients presenting with critical limb ischemia (Rutherford IV-VI) undergoing tibial bypass surgery was performed. Macroperfusion was investigated by measurement of ankle-brachial index. For assessment of microperfusion, intraoperative fluorescence angiography (SPY Elite, NOVADAQ) was undertaken during general anesthesia immediately before and after operation. For each investigation, a fluorescence dye (Indocyanine green) with an amount of 0.1 mg/kg was administered intravenously. Follow-up investigations were performed to assess the clinical outcome.The mean follow-up time was 8.28 (±4.46) months. The parameters Ingress (IN) and Ingress rate (InR) of the fluorescence dye representing the microcirculation showed a significant improvement to preoperative values and correlated with the ankle-brachial index significantly. Furthermore, a significant difference between the individual improvement of the microcirculation parameters (preoperative-postoperative) between patients with postoperative clinical improvement (wound healing, absence of rest pain) and those without could be demonstrated (InR: P = 0.039, IN: P = 0.036).The parameters IN and InR significantly reproduced the postoperative improvement of the microcirculation. An increased ankle-brachial index significantly correlated with increased microcirculation parameters. A better clinical outcome was found in those patients who had a higher relative improvement of microcirculation postoperatively.}, author = {Rother, Ulrich and Lang, Werner and Horch, Raymund E. and Ludolph, Ingo and Meyer, Alexander and Regus, Susanne}, doi = {10.1016/j.avsg.2016.07.084}, faupublication = {yes}, journal = {Annals of Vascular Surgery}, note = {EVALuna2:15278}, pages = {190-197}, peerreviewed = {Yes}, title = {{Microcirculation} {Evaluated} by {Intraoperative} {Fluorescence} {Angiography} after {Tibial} {Bypass} {Surgery}}, volume = {40}, year = {2017} } @article{faucris.117428784, abstract = {Reconstructive microsurgery is an essential part of plastic surgery. To live up to the high technical demands of today's sophisticated techniques, a structured microsurgical training is required. However, such curricula are rare in Germany. We therefore evaluated the concepts and requests of trainers as well as trainees regarding an optimal microsurgical training. We found that the demands of both sides to be fairly similar. How-ever, there were factors potentially hindering the implementation of such curricula, foremost the increasing economic pressure. Based on our findings, representatives of microsurgical trainers and trainees, together with national societies might be able to establish a national curriculum for microsurgical training. The clinical implementation of such a structured train-ing will require significant personal resources. However, this expenditure seems to be justified by the increasing complexity of techniques, the rising demand of patients and the limited time for -surgical training.}, author = {Kolbenschlag, J. and Gehl, B. and Daigeler, A. and Kremer, T. and Hirche, C. and Vogt, P. M. and Horch, Raymund E. and Lehnhardt, M. and Kneser, U.}, doi = {10.1055/s-0034-1381996}, faupublication = {yes}, journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie}, note = {EVALuna2:15575}, pages = {234-41}, peerreviewed = {Yes}, title = {{Microsurgical} training in {Germany} - results of a survey among trainers and trainees}, volume = {46}, year = {2014} } @article{faucris.273228781, abstract = {Decellularized whole muscle constructs represent an ideal scaffold for muscle tissue engineering means as they retain the network and proteins of the extracellular matrix of skeletal muscle tissue. The presence of a vascular pedicle enables a more efficient perfusion-based decellularization protocol and allows for subsequent recellularization and transplantation of the muscle construct in vivo. The goal of this study was to create a baseline for transplantation of decellularized whole muscle constructs by establishing an animal model for investigating a complete native muscle isolated on its pedicle in terms of vascularization and functionality. The left medial gastrocnemius muscles of 5 male Lewis rats were prepared and raised from their beds for in situ muscle stimulation. The stimulation protocol included twitches, tetanic stimulation, fatigue testing, and stretching of the muscles. Peak force, maximum rate of contraction and relaxation, time to maximum contraction and relaxation, and maximum contraction and relaxation rate were determined. Afterwards, muscles were explanted and transplanted heterotopically in syngeneic rats in an isolation chamber by microvascular anastomosis. After 2 weeks, transplanted gastrocnemius muscles were exposed and stimulated again followed by intravascular perfusion with a contrast agent for mu CT analysis. Muscle constructs were then paraffin embedded for immunohistological staining. Peak twitch and tetanic force values all decreased significantly after muscle transplantation while fatigue index and passive stretch properties did not differ between the two groups. Vascular analysis revealed retained perfused vessels most of which were in a smaller radius range of up to 20 mu m and 45 mu m. In this study, a novel rat model of heterotopic microvascular muscle transplantation in an isolation chamber was established. With the assessment of in situ muscle contraction properties as well as vessel distribution after 2 weeks of transplantation, this model serves as a base for future studies including the transplantation of perfusion-decellularized muscle constructs.}, author = {Cai, Aijia and Zheng, Zengming and Müller-Seubert, Wibke and Biggemann, Jonas and Fey, Tobias and Beier, Justus P. and Horch, Raymund E. and Friess, Benjamin and Arkudas, Andreas}, doi = {10.3390/jpm12030442}, faupublication = {yes}, journal = {Journal of Personalized Medicine}, keywords = {muscle transplantation; rat gastrocnemius; in situ stimulation; muscle contraction; perfusion-decellularization}, note = {CRIS-Team WoS Importer:2022-04-15}, peerreviewed = {Yes}, title = {{Microsurgical} {Transplantation} of {Pedicled} {Muscles} in an {Isolation} {Chamber}-{A} {Novel} {Approach} to {Engineering} {Muscle} {Constructs} via {Perfusion}-{Decellularization}}, volume = {12}, year = {2022} } @article{faucris.274945997, abstract = {The arteriovenous (AV) loop model is a key technique to solve one of the major problems of tissue engineering-providing adequate vascular support for a tissue construct of significant size. However, the molecular and cellular mechanisms of vascularization and factors influencing the generation of new tissue in the AV loop are still poorly understood. We previously established a novel intravital microscopy approach to study these events. In this study, we implanted our observation chamber filled with two types of hydrogels such as fibrin and methacrylate gelatin (GelMA) and performed intravital microscopy (IVM) on days 7, 14, and 21. Initial microvessel formation was observed in GelMA on day 14, while the vessel network showed clear indicators of network rearrangement and maturation on day 21. No visible microvessels were observed in fibrin. The chambers were explanted on day 21. Histological examination revealed higher numbers of microvessels in GelMA compared to fibrin, while the AV loop was thrombosed in all fibrin constructs, possibly due to matrix degradation. GelMA proved to be an ideal matrix for IVM studies in the AV loop model due to its slow degradation and transparency. This IVM model can be employed as a novel tool for live and thus faster comprehension of crucial events in the tissue regeneration process, which can improve tissue engineering application.}, author = {Vaghela, Ravikumar and Arkudas, Andreas and Gage, Daniel and Körner, Carolin and Von Hoersten, Stephan and Salehi, Sahar and Horch, Raymund E. and Hessenauer, Maximilian}, doi = {10.1002/jbm.a.37395}, faupublication = {yes}, journal = {Journal of Biomedical Materials Research Part A}, note = {CRIS-Team WoS Importer:2022-05-13}, peerreviewed = {Yes}, title = {{Microvascular} development in the rat arteriovenous loop model in vivo-{A} step by step intravital microscopy analysis}, year = {2022} } @article{faucris.120790604, abstract = {To investigate whether abdominopelvic hemorrhage shown on computed tomography (CT) images can be diagnosed with the same accuracy on a tablet computer as on a dedicated reading display. One hundred patients with a clinical suspicion of abdominopelvic hemorrhage that underwent biphasic CT imaging were retrospectively read by two readers on a dedicated reading display (reference standard) and on a tablet computer (iPad Air). Reading was performed in a dedicated reading room with ambient light conditions. Image evaluation included signs of an active hemorrhage (extravasation of contrast media) and different signs indicating a condition after abdominopelvic hemorrhage (hematoma, intestinal clots, vessel stump, free abdominopelvic fluid with a mean Hounsfield unit value >20, and asymmetric muscle volume indicating intramuscular hemorrhage). Sensitivity, specificity, and positive and negative predictive values (PPV/NPV) were calculated for the tablet-based reading. Active abdominopelvic hemorrhage (n = 72) was diagnosed with the tablet computer with a sensitivity of 0.96, a specificity of 0.93, a PPV of 0.97, and an NPV of 0.90. The results for the detection of the signs indicating a condition after abdominopelvic hemorrhage range from 0.83 to 1.00 in the case of sensitivity, from 0.95 to 1.00 in the case of specificity, from 0.94 to 1.00 in the case of the PPV, and from 0.96 to 1.00 in the case of the NPV. Abdominopelvic hemorrhage shown on CT images can be diagnosed on a tablet computer with a high diagnostic accuracy allowing mobile on-call diagnoses. This may be helpful because an early and reliable diagnosis at any time is crucial for an adequate treatment strategy.}, author = {Schlechtweg, Philipp and Kammerer, Ferdinand J. and Seuß, Hannes and Uder, Michael and Hammon, Matthias}, doi = {10.1007/s10278-015-9829-x}, faupublication = {yes}, journal = {Journal of Digital Imaging}, note = {EVALuna2:14361}, peerreviewed = {Yes}, title = {{Mobile} {Image} {Interpretation}: {Diagnostic} {Performance} of {CT} {Exams} {Displayed} on a {Tablet} {Computer} in {Detecting} {Abdominopelvic} {Hemorrhage}}, year = {2015} } @article{faucris.290414526, abstract = {This study aims to automatically detect and segment the pancreas in portal venous phase contrast-enhanced computed tomography (CT) images. The institutional review board of the University of Erlangen-Nuremberg approved this study and waived the need for informed consent. Discriminative learning is used to build a pancreas tissue classifier incorporating spatial relationships between the pancreas and surrounding organs and vessels. Furthermore, discrete cosine and wavelet transforms are used to build texture features to describe local tissue appearance. Classification is used to guide a constrained statistical shape model to fit the data. The algorithm to detect and segment the pancreas was evaluated on 40 consecutive CT data that were acquired in the portal venous contrast agent phase. Manual segmentation of the pancreas was carried out by experienced radiologists and served as reference standard. Threefold cross validation was performed. The algorithm-based detection and segmentation yielded an average surface distance of 1.7 mm and an average overlap of 61.2 % compared with the reference standard. The overall runtime of the system was 20.4 min. The presented novel approach enables automatic pancreas segmentation in portal venous phase contrast-enhanced CT images which are included in almost every clinical routine abdominal CT examination. Reliable pancreatic segmentation is crucial for computer-aided detection systems and an organ-specific decision support. © 2013 The Author(s).}, author = {Hammon, Matthias and Cavallaro, Alexander Josef and Erdt, Marius and Dankerl, Peter and Kirschner, Matthias and Drechsler, Klaus and Wesarg, Stefan and Uder, Michael and Janka, Rolf Matthias}, doi = {10.1007/s10278-013-9586-7}, faupublication = {yes}, journal = {Journal of Digital Imaging}, keywords = {Computed tomography; Computer-assisted detection; Machine learning; Pancreas; Segmentation}, note = {CRIS-Team Scopus Importer:2023-03-08}, pages = {1082-1090}, peerreviewed = {Yes}, title = {{Model}-based pancreas segmentation in portal venous phase contrast-enhanced {CT} images}, volume = {26}, year = {2013} } @article{faucris.262967971, abstract = {Alginate hydrogels have been used as a biomaterial for 3D culturing for several years. Here, gene expression patterns in melanoma cells cultivated in 3D alginate are compared to 2D cultures. It is well-known that 2D cell culture is not resembling the complex in vivo situation well. However, the use of very intricate 3D models does not allow performing high-throughput screening and analysis is highly complex. 3D cell culture strategies in hydrogels will better mimic the in vivo situation while they maintain feasibility for large-scale analysis. As alginate is an easy-to-use material and due to its favorable properties, it is commonly applied as a bioink component in the growing field of cell encapsulation and biofabrication. Yet, only a little information about the transcriptome in 3D cultures in hydrogels like alginate is available. In this study, changes in the transcriptome based on RNA-Seq data by cultivating melanoma cells in 3D alginate are analyzed and reveal marked changes compared to cells cultured on usual 2D tissue culture plastic. Deregulated genes represent valuable cues to signaling pathways and molecules affected by the culture method. Using this as a model system for tumor cell plasticity and heterogeneity, EGR1 is determined to play an important role in melanoma progression.}, author = {Kappelmann-Fenzl, M. and Schmidt, Sonja and Fischer, Stefan and Schmid, Rafael and Lämmerhirt, Lisa and Fischer, Lena and Schrüfer, Stefan and Thievessen, Ingo and Schubert, Dirk W. and Matthies, Alexander Oliver and Detsch, Rainer and Boccaccini, Aldo R. and Arkudas, Andreas and Kengelbach-Weigand, Annika and Boßerhoff, Anja Katrin}, doi = {10.3390/cancers13164111}, faupublication = {yes}, journal = {Cancers}, keywords = {3D cultivation; Biomaterials; EGR1; Melanoma; Whole transcriptome}, note = {CRIS-Team Scopus Importer:2021-08-20}, pages = {72-72}, peerreviewed = {Yes}, title = {{Molecular} changes induced in melanoma by cell culturing in {3D} alginate hydrogels}, volume = {13}, year = {2021} } @inproceedings{faucris.280359828, address = {NEW ROCHELLE}, author = {Robering, Jan W. and Reakasame, Supachai and Gao, Jie and Beier, Justus P. and Boccaccini, Aldo R. and Horch, Raymund E. and Boos, Anja M.}, booktitle = {TISSUE ENGINEERING PART A}, faupublication = {yes}, note = {CRIS-Team WoS Importer:2022-08-12}, pages = {S341-S341}, peerreviewed = {unknown}, publisher = {MARY ANN LIEBERT, INC}, title = {{MOULDING} {THE} {FUTURE} {OF} {3D} {PRINTING}: {SACRIFICIAL} {MOULDS} {FOR} {THE} {FABRICATION} {OF} {HYDROGEL} {CONSTRUCTS}}, year = {2022} } @article{faucris.117822144, abstract = {Collagen I is the major fibrous extracellular component of bone responsible for its ultimate tensile strength. In tissue engineering, one of the most important challenges for tissue formation is to get cells interconnected via a strong and functional extracellular matrix (ECM), mimicking as closely as possible the natural ECM geometry. Still missing in tissue engineering are: (a) a versatile, high-resolution and non-invasive approach to evaluate and quantify different aspects of ECM development within engineered biomimetic scaffolds online; and (b) deeper insights into the mechanism whereby cellular matrix production is enhanced in 3D cell-scaffold composites, putatively via enhanced focal adhesion linkage, over the 2D setting. In this study, we developed sensitive morphometric detection methods for collagen I-producing and bone-forming mesenchymal stem cells (MSCs), based on multiphoton second harmonic generation (SHG) microscopy, and used those techniques to compare collagen I production capabilities in 2D- and 3D-arranged cells. We found that stimulating cells with 1% serum in the presence of ascorbic acid is superior to other medium conditions tested, including classical osteogenic medium. In contrast to conventional 2D culture, having MSCs packed closely in a 3D environment presumably stimulates cells to produce strong and complex collagen I networks with defined network structures (visible in SHG images) and improves collagen production. Copyright © 2015 John Wiley & Sons, Lt}, author = {Vielreicher, Martin and Gellner, Monika and Rottensteiner, Ulrike and Horch, Raymund E. and Arkudas, Andreas and Friedrich, Oliver}, doi = {10.1002/term.2107}, faupublication = {yes}, journal = {Journal of Tissue Engineering and Regenerative Medicine}, pages = {2104-2115}, peerreviewed = {Yes}, title = {{Multiphoton} microscopy analysis of extracellular collagen {I} network formation by mesenchymal stem cells.}, volume = {11}, year = {2015} } @article{faucris.272197092, abstract = {Objective: Decellularizing solid organs is a promising top-down process to produce acellular bio-scaffolds for 'de novo' regrowth or application as tissue 'patches' that compensate, e.g., large volumetric muscle loss in reconstructive surgery. Therefore, generating standardized acellular muscle scaffolds marks a pressing area of need. Although animal muscle decellularization protocols were established, those are mostly manually performed and lack defined bioreactor environments and metrologies to assess decellularization quality in real-time. To close this gap, we engineered an automated bioreactor system to provide chemical decellularization solutions to immersed whole rat gastrocnemius medialis muscle through perfusion of the main feeding arteries. Results: Perfusion control is adjustable according to decellularization quality feedback. This was assessed both from (i) ex situ assessment of sarcomeres/nuclei through multiphoton fluorescence and label-free Second Harmonic Generation microscopy and DNA quantification, along with (ii) in situ assessment of the samples passive mechanical elasticity. Conclusion: We find DNA and sarcomere-free constructs after 72 h of 0.1% SDS perfusion-decellularization. Furthermore, passive elasticity can be implemented as additional online decellularization quality measure, noting a threefold elasticity decrease in acellular constructs. Significance: Our MyoBio represents a novel and useful automated bioreactor environment for standardized and controlled generation of acellular whole muscle scaffolds as a valuable source for regenerative medicine.}, author = {Ritter, Paul and Cai, Aijia and Reischl, Barbara and Fiedler, Maren and Prölß, Gerhard and Frie, Benjamin and Kretzschmar, Elke and Michael, Mena and Hartmann, Kristin and Lesko, Christian and Salti, Hatiham and Arkudas, Andreas and Horch, Raymund E. and Paulsen, Friedrich and Friedrich, Oliver and Haug, Michael}, doi = {10.1109/TBME.2022.3142317}, faupublication = {yes}, journal = {IEEE transactions on bio-medical engineering}, keywords = {Biological systems; Biomechatronics; bioreactors; Biotechnology; decellularization; Inductors; Muscles; Plastics; skeletal muscle; Surgery; Tendons; tissue engineering}, month = {Jan}, note = {CRIS-Team Scopus Importer:2022-04-01}, peerreviewed = {unknown}, title = {{MyoBio}: {An} automated bioreactor system technology for standardized perfusion-decellularization of whole skeletal muscle}, year = {2022} } @article{faucris.111030744, author = {Horch, Raymund E. and Hohenberger, Werner and Weber, Klaus and Arkudas, Andreas and Beier, Justus}, doi = {10.1007/s00384-016-2543-4}, faupublication = {yes}, journal = {International Journal of Colorectal Disease}, note = {EVALuna2:15233}, pages = {1525-7}, peerreviewed = {Yes}, title = {{Myocutaneous} transpelvic flaps do improve quality of life and help to reduce wound healing complications in patients receiving abdominoperineal resection in the real world}, volume = {31}, year = {2016} } @article{faucris.302871024, abstract = {BACKGROUND: For the purpose of skeletal muscle engineering, primary myoblasts (Mb) and adipogenic mesenchymal stem cells (ADSC) can be co-cultured and myogenically differentiated. Electrospun composite nanofiber scaffolds represent suitable matrices for tissue engineering of skeletal muscle, combining both biocompatibility and stability Although growth differentiation factor 11 (GDF11) has been proposed as a rejuvenating circulating factor, restoring skeletal muscle function in aging mice, some studies have also described a harming effect of GDF11. Therefore, the aim of the study was to analyze the effect of GDF11 on co-cultures of Mb and ADSC on poly-ε-caprolactone (PCL)-collagen I-polyethylene oxide (PEO)-nanofibers. RESULTS: Human Mb were co-cultured with ADSC two-dimensionally (2D) as monolayers or three-dimensionally (3D) on aligned PCL-collagen I-PEO-nanofibers. Differentiation media were either serum-free with or without GDF11, or serum containing as in a conventional differentiation medium. Cell viability was higher after conventional myogenic differentiation compared to serum-free and serum-free + GDF11 differentiation as was creatine kinase activity. Immunofluorescence staining showed myosine heavy chain expression in all groups after 28 days of differentiation without any clear evidence of more or less pronounced expression in either group. Gene expression of myosine heavy chain (MYH2) increased after serum-free + GDF11 stimulation compared to serum-free stimulation alone. CONCLUSIONS: This is the first study analyzing the effect of GDF11 on myogenic differentiation of Mb and ADSC co-cultures under serum-free conditions. The results of this study show that PCL-collagen I-PEO-nanofibers represent a suitable matrix for 3D myogenic differentiation of Mb and ADSC. In this context, GDF11 seems to promote myogenic differentiation of Mb and ADSC co-cultures compared to serum-free differentiation without any evidence of a harming effect.}, author = {Cai, Aijia and Schneider, Paul and Zheng, Zengming and Beier, Justus P. and Himmler, Marcus and Schubert, Dirk W. and Weisbach, Volker Günter and Horch, Raymund E. and Arkudas, Andreas}, doi = {10.1186/s12860-023-00478-1}, faupublication = {yes}, journal = {BMC Molecular and Cell Biology}, keywords = {ADSC; Collagen; Electrospun composite nanofibers; GDF11; Myoblasts; Myogenic differentiation; Myostatin; Nanoscaffolds; poly-ε-caprolacton (PCL); Polyethylene oxide (PEO)}, note = {CRIS-Team Scopus Importer:2023-05-26}, pages = {18-}, peerreviewed = {Yes}, title = {{Myogenic} differentiation of human myoblasts and {Mesenchymal} stromal cells under {GDF11} on {NPoly}-ɛ-caprolactone-collagen {I}-{Polyethylene}-nanofibers}, volume = {24}, year = {2023} } @article{faucris.217720587, abstract = {The creation of functional skeletal muscle via tissue engineering holds great promise without sacrificing healthy donor tissue. Different cell types have been investigated regarding their myogenic differentiation potential under the influence of various media supplemented with growth factors. Yet, most cell cultures include the use of animal sera, which raises safety concerns and might lead to variances in results. Electrospun nanoscaffolds represent suitable matrices for tissue engineering of skeletal muscle, combining both biocompatibility and stability.
We therefore aimed to develop a
serum-free myogenic differentiation medium for the co-culture of
primary myoblasts (Mb) and mesenchymal stromal cells derived from the
bone marrow (BMSC) and adipose tissue (ADSC) on electrospun
poly-ε-caprolacton (PCL)-collagen I-nanofiber},
author = {Arkudas, Andreas and Weigand, Annika and Beier, Justus and Horch, Raymund E. and Hardt, Moritz and Schneider, Paul and Schmid, Rafael and Lange, Claudia and Dippold, Dirk and Schubert, Dirk W. and Boos, Anja and Cai, Aijia},
doi = {10.1186/s12896-018-0482-6},
faupublication = {yes},
journal = {BMC Biotechnology},
keywords = {Nanoscaffolds; Myogenic differentiation; Myoblasts; Mesenchymal stromal cells; Serum-free media; BMSC; Electrospun PCL-collagen I-nanofibers; ADSC},
peerreviewed = {Yes},
title = {{Myogenic} differentiation of primary myoblasts and mesenchymal stromal cells under serum-free conditions on {PCL}-collagen {I}-nanoscaffolds},
url = {https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12896-018-0482-6},
volume = {18},
year = {2018}
}
@article{faucris.210009031,
abstract = {Background: The creation of functional skeletal muscle via tissue engineering holds great promise without sacrificing healthy donor tissue. Different cell types have been investigated regarding their myogenic differentiation potential under the influence of various media supplemented with growth factors. Yet, most cell cultures include the use of animal sera, which raises safety concerns and might lead to variances in results. Electrospun nanoscaffolds represent suitable matrices for tissue engineering of skeletal muscle, combining both biocompatibility and stability. We therefore aimed to develop a serum-free myogenic differentiation medium for the co-culture of primary myoblasts (Mb) and mesenchymal stromal cells derived from the bone marrow (BMSC) and adipose tissue (ADSC) on electrospun poly-ε-caprolacton (PCL)-collagen I-nanofibers. Results: Rat Mb were co-cultured with rat BMSC (BMSC/Mb) or ADSC (ADSC/Mb) two-dimensionally (2D) as monolayers or three-dimensionally (3D) on aligned PCL-collagen I-nanofibers. Differentiation media contained either AIM V, AIM V and Ultroser® G, DMEM/Ham’s F12 and Ultroser® G, or donor horse serum (DHS) as a conventional differentiation medium. In 2D co-culture groups, highest upregulation of myogenic markers could be induced by serum-free medium containing DMEM/Ham’s F12 and Ultroser® G (group 3) after 7 days. Alpha actinin skeletal muscle 2 (ACTN2) was upregulated 3.3-fold for ADSC/Mb and 1.7-fold for BMSC/Mb after myogenic induction by group 3 serum-free medium when compared to stimulation with DHS. Myogenin (MYOG) was upregulated 5.2-fold in ADSC/ Mb and 2.1-fold in BMSC/Mb. On PCL-collagen I-nanoscaffolds, ADSC showed a higher cell viability compared to BMSC in co-culture with Mb. Myosin heavy chain 2, ACTN2, and MYOG as late myogenic markers, showed higher gene expression after long term stimulation with DHS compared to serum-free stimulation, especially in BMSC/Mb cocultures. Immunocytochemical staining with myosin heavy chain verified the presence of a contractile apparatus under both serum free and standard differentiation conditions. Conclusions: In this study, we were able to myogenically differentiate mesenchymal stromal cells with myoblasts on PCL-collagen I-nanoscaffolds in a serum-free medium. Our results show that this setting can be used for skeletal muscle tissue engineering, applicable to future clinical applications since no xenogenous substances were used.
This review summarizes the knowledge on rupture (in vivo as well as under laboratory conditions) of gel-filled silicone breast implants, which has been an open question since the first implantation in 1962. We present recent progress which has been made by a mapping of the mechanical properties of the shell material together with a statistical failure analysis by means of a Weibull fit. Reference implants and intact as well as ruptured explants are tested after several years in vivo using tensile tests. Up to 110 dumbbell-shaped specimens per implant are measured allowing a detailed mapping of the mechanical properties of the silicone shell. Therefore, it is possible to illustrate the results of the tensile tests by mapping the measured values of the implants in the form of a contour plot revealing novel insights. The contour plots clearly display that the mechanical behaviour (e.g. stress at break) varies significantly over the shell. Additionally, a map is proposed which clearly shows separated clusters for different manufacturers and product categories. © 2013 Society of Chemical Industry},
author = {Schubert, Dirk W. and Kaschta, Joachim and Horch, Raymund E. and Walter, Bastian and Daenicke, Jonas},
doi = {10.1002/pi.4619},
faupublication = {yes},
journal = {Polymer International},
note = {UnivIS-Import:2015-03-09:Pub.2013.tech.FT.FT-TM.onthef},
pages = {172-178},
peerreviewed = {Yes},
title = {{On} the failure of silicone breast implants: {New} insights by mapping the mechanical properties of implant shells},
volume = {63},
year = {2014}
}
@article{faucris.218416174,
abstract = {Background Body contouring surgery after massive weight loss was shown to ameliorate the patient's quality of life and to enhance physical and psychological well-being. However, numerous patients are still obese when presenting for body contouring surgery, not able to lose additional weight for various reasons. Data regarding general feasibility, outcome, and postoperative complications in obese patients is rare. The aim of this study was to investigate the outcome in body contouring procedures in obese patients.},
author = {Hauck, Theresa and Schmitz, Marweh and Horch, Raymund E. and Arkudas, Andreas and Boos, Anja and Ludolph, Ingo and Cai, Aijia},
doi = {10.1007/s11695-018-03697-0},
faupublication = {yes},
journal = {Obesity Surgery},
note = {CRIS-Team WoS Importer:2019-05-24},
pages = {1563-1570},
peerreviewed = {Yes},
title = {{Operating} on the {Edge}? {Body} {Contouring} {Procedures} in {Patients} with {Body} {Mass} {Index} {Greater} 35},
volume = {29},
year = {2019}
}
@article{faucris.111007864,
abstract = {Management of dialysis patients with critical limb ischemia (CLI) still represents a challenge to vascular medicine, whereas the effects of the pedal arch quality in these patients with predominant affection of the infrapopliteal vessels have rarely been evaluated. Therefore, our aim was to analyze the outcomes of infrapopliteal angioplasty in the setting of chronic renal failure (end-stage renal disease) and evaluate the influence of the pedal arch involvement on clinical success.Prospective follow-up of 32 ERSD patients on hemodialysis (mean age, 72 years) with CLI and consecutive infrapopliteal angioplasty over a 5-year period 2010-2014 was performed. Mean follow-up was 10 months (range, 0-51 months). Statistical end points were defined for amputation-free survival, overall survival, and wound healing. Each patient's pedal arch was classified in 4 categories according to patency on completion angiography and the influence of the pedal arch quality on end points was assessed.A total of 44 vessels in 32 ischemic legs were treated. Technical success was achieved in 96% of patients, no major complications were observed. A 30-day mortality rate amounted 6% with no procedure-related deaths. The 1-year amputation-free survival rate was 56% and 34% at 2 years. Two major amputations were required. Subsequent revascularization procedures were necessary in 11 patients (10 redo angioplasty, 1 pedal bypass graft). The pedal arch was classified as category I in 1 patient (3%), category IIa in 12 (38%), IIb in 3 (9%), and III in 16 patients (50%). No statistical significant differences in terms of survival or wound-healing rate were observed between those groups, and the pedal arch quality had no impact on predefined end points.End-stage renal disease patients represent a subgroup with poor prognosis of limb salvage in CLI. Amputation-free survival remains poor and based on these data, an endovascular therapy is feasible and safe in these highly multimorbid patients. The quality of the pedal arch was not found to have any impact on wound healing or survival in the present study.},
author = {Meyer, Alexander and Schinz, Katharina and Lang, Werner and Schmid, Andrea and Regus, Susanne and Rother, Ulrich},
doi = {10.1016/j.avsg.2016.01.039},
faupublication = {yes},
journal = {Annals of Vascular Surgery},
note = {EVALuna2:14613},
pages = {121-9},
peerreviewed = {Yes},
title = {{Outcomes} and {Influence} of the {Pedal} {Arch} in {Below}-the-{Knee} {Angioplasty} in {Patients} with {End}-{Stage} {Renal} {Disease} and {Critical} {Limb} {Ischemia}},
volume = {35},
year = {2016}
}
@article{faucris.253003812,
abstract = {While autologous breast reconstruction has gained momentum over recent years, there is limited data on the structure and quality of care of microsurgical breast reconstruction in Germany. Using the breast reconstruction database established by the German Society of Plastic, Reconstructive and Aesthetic Surgeons (DGPRaC), the presented study investigated the overall outcomes of deep inferior epigastric perforator (DIEP) flap reconstructions in Germany. Data of 3926 patients and 4577 DIEP flaps performed by 22 centers were included in this study. Demographics, patient characteristics, perioperative details and postoperative outcomes were accounted for. Centers performing < o 40 (low-volume (LV)) vs. >= o 40 (high-volume (HV)) annual DIEP flaps were analyzed separately. Overall, total and partial flap loss rates were as low as 2.0% and 1.1% respectively, and emergent vascular revision surgery was performed in 4.3% of cases. Revision surgery due to wound complications was conducted in 8.3% of all cases. Mean operative time and length of hospital stay was significantly shorter in the HV group (LV: 385.82 min vs. HV: 287.14 min; LV: 9.04 (18.87) days vs. HV: 8.21 (5.04) days; both p < 0.05). The outcome and complication rates deduced from the national registry underline the high standard of microsurgical breast reconstruction on a national level in Germany.},
author = {Heidekrueger, Paul I. and Moellhoff, Nicholas and Horch, Raymund E. and Lohmeyer, Joern A. and Marx, Mario and Heitmann, Christoph and Fansa, Hisham and Geenen, Matthias and Gabka, Christian J. and Handstein, Steffen and Prantl, Lukas and Von Fritschen, Uwe},
doi = {10.3390/jcm10051016},
faupublication = {yes},
journal = {Journal of Clinical Medicine},
note = {CRIS-Team WoS Importer:2021-03-26},
peerreviewed = {Yes},
title = {{Overall} {Complication} {Rates} of {DIEP} {Flap} {Breast} {Reconstructions} in {Germany}-{A} {Multi}-{Center} {Analysis} {Based} on the {DGPRaC} {Prospective} {National} {Online} {Registry} for {Microsurgical} {Breast} {Reconstructions}},
volume = {10},
year = {2021}
}
@article{faucris.204501618,
abstract = {Injury to the central slip of the extensor tendon may occur with open and also with closed injuries, such as volar dislocation of the proximal interphalangeal (PIP) joint. For adequate treatment, it is necessary to identify all injured structures. Without appropriate primary management, the patient is likely to develop a subacute to chronic posttraumatic boutonnière deformity. A fixed boutonnière deformity requires recovery of joint mobility. Once joint mobility is achieved, secondary surgical reconstruction of the central slip can be performed with a tendon transfer or a tendon transplant.},
author = {Hauck, Theresa and Müller-Seubert, Wibke and Horch, Raymund E.},
doi = {10.1007/s00113-017-0404-4},
faupublication = {yes},
journal = {Unfallchirurg},
note = {EVALuna2:32836},
pages = {873-884},
peerreviewed = {Yes},
title = {{Palmar} dislocation of the proximal interphalangeal joint and traumatic boutonnière deformity},
volume = {120},
year = {2017}
}
@article{faucris.270411788,
abstract = {Aufgrund der Fähigkeit, die komplexe Anatomie der Hand und des Handgelenks detailliert abzubilden, ist die Magnetresonanztomografie (MRT) ein ideales bildgebendes Verfahren für die Analyse vieler unterschiedlicher Traumata und Pathologien. Die MRT wird häufig nach initialer Röntgen- und Ultraschalldiagnostik ergänzend durchgeführt. Zuletzt erfolgten pro Jahr ca. 10 Millionen MRT-Untersuchungen in Deutschland [1]. Zu den häufigsten Indikationen für die Durchführung einer MRT-Untersuchung der Hand zählen: Beurteilung der intrinsischen Bänder des Handgelenks und des triangulären fibrokartilaginären Komplexes (TFCC), Charakterisierung von Raumforderungen (z. B. Ganglien oder Weichteiltumore), Detektion von Beuge- und Strecksehnenverletzungen und Kapsel-/Bandläsionen an den Fingern sowie Nachweis okkulter Knochenverletzungen [2], [3], [4].
Damit die Anatomie der einzelnen Strukturen der Hand bestmöglich abgebildet wird und Bewegungsartefakte möglichst gering gehalten werden können, erfordert dies eine Optimierung der Platzierung der Hand in der gewählten Spule sowie der Bildgebungsparameter (u. a. Schichtdicke, Sichtfeld und Repetitionszeit/Echozeit). Regulär werden Patienten in Bauchlage mit über dem Kopf ausgestrecktem Arm in der „Superman“-Position gelagert, sodass die Hand für eine bestmögliche, homogene Bildqualität im Isozentrum des Magnetfelds positioniert wird. Dies ist jedoch für manche Patienten unangenehm, und einige Patienten sind nicht im Stande, die MRT-Untersuchung in dieser Lagerung zu vollenden [5].
Im Folgenden berichten wir über einen außergewöhnlichen Fall, bei dem es im Rahmen einer MRT-Untersuchung der rechten Hand zu einer relevanten Veränderung der Perfusionsverhältnisse an der linken Hand gekommen war, bei vorausgegangener „Superman“-Lagerung während der MRT-Untersuchun}, author = {Promny, Dominik and Grüner, Jasmin and Hauck, Theresa and Horch, Raymund E.}, doi = {10.1055/a-1738-9906}, faupublication = {yes}, journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie}, note = {CRIS-Team WoS Importer:2022-03-04}, peerreviewed = {unknown}, title = {{Paradoxical} {Perfusion} {Reaction} of the contralateral {Hand} after {Hand} {MRI}}, year = {2022} } @article{faucris.229368886, abstract = {Background and objectives: The purpose of this study is to analyze major complication rates and different aspects of health-related quality of life (HRQoL) in extremity soft tissue sarcoma (STS) patients treated with or without radio (chemo) therapy and surgery. Methods: We performed a retrospective analysis of all patients who underwent Extremity STS excision from 2004 to 2014 (182 patients included). Patients' data were collected from patients' records. HRQoL was assessed by using EORTC QLQ-C30. Results: A total of 182 patients underwent sarcoma resection. After neoadjuvant radiochemotherapy (RCT), the major-complication rate amounted to 28% (vs. 7%, no radiotherapy, p < 0.001). Major-complication rates after adjuvant radiotherapy (RT) occurred in 8% (vs. 7%, no radiotherapy, p = 0.265). Comparison QoL scores between treating with neoadjuvant RCT or without RT revealed significant worse scores with neoadjuvant RCT. Further stratification of disease control of these patients showed significant reduced scores in the group of disease-free patients with neoadjuvant RCT compared to irradiated disease-free patients. Discussion: To date, there have only been a few investigations of QoL in STS. Retrospective study on quality of life have limitations, like a lack of baseline evaluation of QoL. Patient candidated to radiation therapy could have had worse QoL baseline due to more advanced disease. Disease status of the patients who answered the questionnaires could have been an influence of QoL and we could show reduced scores in the group of disease-free patients with neoadjuvant RCT, but not for the patients with recurrence or metastasis, so it is very hard to discriminate whether radiation therapy could really have an impact or not. Conclusion: This study might assist in further improving the understanding of QoL in STS patients and may animate for prospective studies examining the oncological therapies impact on HRQoL.}, author = {Götzl, Rebekka and Sterzinger, Sebastian and Semrau, Sabine and Vassos, Nikolaos and Hohenberger, Werner and Grützmann, Robert and Agaimy, Abbas and Arkudas, Andreas and Horch, Raymund E. and Beier, Justus}, doi = {10.1186/s12955-019-1236-4}, faupublication = {yes}, journal = {Health and Quality of Life Outcomes}, keywords = {Health related quality of life; QLQ-C30; Sarcoma major complication rates; Sarcoma radiotherapy; Soft tissue sarcoma}, note = {CRIS-Team Scopus Importer:2019-11-19}, peerreviewed = {Yes}, title = {{Patient}'s quality of life after surgery and radiotherapy for extremity soft tissue sarcoma-a retrospective single-center study over ten years}, volume = {17}, year = {2019} } @article{faucris.120033584, abstract = {
Introduction: Axial vascularization represents a mandatory requirement for clinically applied larger scale vascularized bone grafts. The aim of this study was to combine the arteriovenous (AV) loop model in the rat with a critically sized femoral bone defect and to successfully transplant axially vascularized bone constructs into the defect.
Materials and Methods: In Groups A and C, an AV loop together with a clinically approved hydroxyapatite and beta-tricalcium phosphate (HA/β-TCP) matrix, mesenchymal stem cells, and recombinant human bone morphogenetic protein 2 were implanted into a newly designed porous titanium chamber with an integrated osteosynthesis plate in the thighs of rats, whereas in Groups B and D, the same matrix composition without AV loop and, in Group E, only the HA/β-TCP matrix were implanted. After 6 weeks, the constructs were transplanted into a 10 mm femoral defect created in the same leg, in Groups A and C, under preservation of the AV loop pedicle. Group F served as a control group with an empty chamber. Ten days (Groups A and B) and 12 weeks (Groups C–F) after transplantation, the femora together with the constructs were explanted and investigated using computed tomography (CT), micro-CT, X-ray, histology, and real-time polymerase chain reaction (RT-PCR).
Results: Ten days after transplantation, Group A showed a maintained vascular supply leading to increased vascularization, cell survival in the scaffold center, and bone generation compared to Group B. After 12 weeks, there was no difference detectable among all groups regarding total vessel number, although Group C, using the AV loop, still showed increased vascularization of the construct center compared to Groups D and E. In Group C, there was still enhanced bone generation detectable compared to the other groups and increased bony fusion rate at the proximal femoral stump.
Conclusions: This study shows the combination of the AV loop model in the rat with a critically sized femoral defect. By maintenance of the vascular supply, the constructs initially showed increased vascularization, leading to increased bone formation and bony fusion in the long term.
}, author = {Arkudas, Andreas and Lipp, Amelie and Bührer, Gregor and Arnold, Isabel and Davinova, Diana and Brandl, Andreas and Beier, Justus and Körner, Carolin and Lyer, Stefan and Alexiou, Christoph and Kneser, Ulrich and Horch, Raymund E.}, doi = {10.1089/ten.tea.2017.0110}, faupublication = {yes}, journal = {Tissue Engineering: Parts A, B, and C}, keywords = {AV loop;femur defect model;critically sized bone defect;bone tissue engineering}, peerreviewed = {Yes}, title = {{Pedicled} {Transplantation} of {Axially} {Vascularized} {Bone} {Constructs} in a {Critical} {Size} {Femoral} {Defect}}, year = {2017} } @article{faucris.122920424, abstract = {The aim of this article is to improve the treatment of patients with complete skin loss of the penile shaft after Fournier's gangrene or hidradenitis suppurativa using modern biomatrices and topical negative pressure therapy. From January 2010 to December 2011, three patients with Fournier's gangrene or hidradenitis suppurativa were treated. After initial radical debridements, topical negative pressure therapy was applied for wound stabilisation. After that dermal templates (acellular dermal matrix) were used to achieve early healing and topical negative pressure-dressing in a special setting or a special foam compression bandage was used together with a urinary catheter. After integration of the dermal template, a split-thickness skin graft is used for coverage and again secured with a circular total negative pressure-dressing. In all cases, the split-thickness skin grafts healed very well applying this therapy concept. The patients were very satisfied with the functional as well as with the aesthetic outcome. We suggest a new method of staged reconstruction to successfully preserve the functionality of the penis after complete loss of the skin and soft tissue of the penile shaft using modern biomatrices and topical negative pressure therapy.}, author = {Ludolph, Ingo and Titel, Torsten and Beier, Justus and Dragu, Adrian and Schmitz, Marweh and Wullich, Bernd and Horch, Raymund E.}, doi = {10.1111/iwj.12235}, faupublication = {yes}, journal = {International Wound Journal}, note = {EVALuna2:15559}, pages = {77-81}, peerreviewed = {Yes}, title = {{Penile} reconstruction with dermal template and vacuum therapy in severe skin and soft tissue defects caused by {Fournier}'s gangrene and hidradenitis suppurativa}, volume = {13}, year = {2016} } @article{faucris.204501850, abstract = {Perioperative management of microsurgery is not well standardised. Due to a lack of evidence, different regimes are established in different microsurgical centres. However, trends towards less aggressive perioperative interventions can be identified in recent years, since treatment algorithms without systematic evidence are being progressively abandoned. The available evidence on perioperative issues, such as temperature control, fluid resuscitation, blood transfusions, application of vasodilators or - pressors, as well as anticoagulants, were discussed during the consensus conference on perioperative management at the annual meeting of the German Speaking Society for Microsurgery of Peripheral Nerves and Vessels. Common basic standards were identified and a consensus was reached that is described in the following manuscript. }, author = {Kremer, T. and Bauer, M. and Zahn, P. and Wallner, C. and Fuchs, P. and Horch, Raymund E. and Schaefer, D. J. and Bader, R. D. and Lehnhardt, M. and Reichert, B. and Pierer, G. and Hirche, C. and Kneser, U.}, doi = {10.1055/s-0042-108806}, faupublication = {yes}, journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie}, note = {EVALuna2:32827}, pages = {205-11}, peerreviewed = {Yes}, title = {{Perioperative} {Management} in {Microsurgery} - {Consensus} {Statement} of the {German} {Speaking} {Society} for {Microsurgery} of {Peripheral} {Nerves} and {Vessels}}, volume = {48}, year = {2016} } @article{faucris.263243511, abstract = {Tissue engineering principles allow the generation of functional tissues for biomedical applications. Reconstruction of large-scale bone defects with tissue-engineered bone has still not entered the clinical routine. In the present study, a bone substitute in combination with mesenchymal stem cells (MSC) and endothelial progenitor cells (EPC) with or without growth factors BMP-2 and VEGF-A was prevascularized by an arteriovenous (AV) loop and transplanted into a critical-size tibia defect in the sheep model. With 3D imaging and immunohistochemistry, we could show that this approach is a feasible and simple alternative to the current clinical therapeutic option. This study serves as proof of concept for using large-scale transplantable, vascularized, and customizable bone, generated in a living organism for the reconstruction of load-bearing bone defects, individually tailored to the patient’s needs. With this approach in personalized medicine for the reconstruction of critical-size bone defects, regeneration of parts of the human body will become possible in the near future.}, author = {Kengelbach-Weigand, Annika and Thielen, Carolina and Bäuerle, Tobias and Götzl, Rebekka and Gerber, Thomas and Körner, Carolin and Beier, Justus and Horch, Raymund E. and Boos, Anja}, doi = {10.1038/s41536-021-00158-8}, faupublication = {yes}, journal = {npj Regenerative Medicine}, note = {CRIS-Team Scopus Importer:2021-08-27}, peerreviewed = {Yes}, title = {{Personalized} medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue}, volume = {6}, year = {2021} } @article{faucris.265764141, abstract = {Non-healing extensive wounds in the perineal region can lead to severe soft tissue infections and disastrous complications, which are not manageable with conservative measures. Specifically in recurrent or advanced pelvic malignancies, irradiation often leads to extensive scarring and wound breakdown, resulting in significant soft tissue defects during surgical tumor excision. Among several surgical options to reconstruct the perineum, the transpelvic vertical rectus abdominis myocutaneous (VRAM) flap has proven to be one of the most reliable methods. Specific modifications of this flap allow an individualized procedure depending on the patient’s needs. We modified this technique to include the urethral orifice into the skin paddle of VRAM flaps in three patients as a novel option to circumvent urinary diversion and maintain an acceptable quality of life.}, author = {Horch, Raymund E. and Ludolph, Ingo and Arkudas, Andreas and Cai, Aijia}, doi = {10.3390/jpm11111076}, faupublication = {yes}, journal = {Journal of Personalized Medicine}, keywords = {Neourethra; Perineal reconstruction; Urethral reconstruction; VRAM flap}, note = {CRIS-Team Scopus Importer:2021-11-05}, peerreviewed = {Yes}, title = {{Personalized} reconstruction of genital defects in complicated wounds with vertical rectus abdominis myocutaneous flaps including urethral neo-orifice}, volume = {11}, year = {2021} } @article{faucris.314634138, abstract = {Human skin is particularly vulnerable to external damaging influences such as irradiation, extreme temperatures, chemical trauma, and certain systemic diseases, which reduce the skin's capacity for regeneration and restoration and can possibly lead to large-scale skin defects. To restore skin continuity in severe cases, surgical interventions such as the transplantation of autologous tissue are needed. Nevertheless, the coverage of larger skin defects caused by severe third-grade burns or extensive irradiation therapy is limited due to the depletion of uninjured autologous tissue. In such cases, many of the patient's epidermal cells can become available using biofabricated skin grafts, thereby restoring the skin's vital functions. Given the limited availability of autologous skin grafts for restoring integrity in large-scale defects, using bioprinted constructs as skin graft substitutes could offer an encouraging therapeutic alternative to conventional therapies for large-scale wounds, such as the transplantation of autologous tissue. Using layer-by-layer aggregation or volumetric bioprinting, inkjet bioprinting, laser-assisted bioprinting, or extrusion-based bioprinting, skin cells are deposited in a desired pattern. The resulting constructs may be used as skin graft substitutes to accelerate wound healing and reconstitute the physiological functions of the skin. In this review, we aimed to elucidate the current state of bioprinting within the context of skin tissue engineering and introduce and discuss different bioprinting techniques, possible approaches and materials, commonly used cell types, and strategies for graft vascularization for the production of bioprinted constructs for use as skin graft substitutes.}, author = {Sörgel, Celena and Cai, Aijia and Schmid, Rafael and Horch, Raymund E.}, doi = {10.3390/biomedicines11102678}, faupublication = {yes}, journal = {Biomedicines}, note = {EVALuna2:542726}, peerreviewed = {Yes}, title = {{Perspectives} on the {Current} {State} of {Bioprinted} {Skin} {Substitutes} for {Wound} {Healing}.}, volume = {11}, year = {2023} } @article{faucris.242394299, abstract = {Objectives Coactivators are a heterogeneous family of transcriptional regulators that are essential for modulation of transcriptional outcomes and fine-tune numerous cellular processes. The aim of the present study was to evaluate the role of the coactivator peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α) in the pathogenesis of systemic sclerosis (SSc). Methods Expression of PGC-1α was analysed by real-time PCR, western blot and immunofluorescence. Modulation of autophagy was analysed by reporter studies by expression of autophagy-related genes. The effects of PGC-1α knockdown on collagen production and myofibroblast differentiation were analysed in cultured human fibroblasts and in two mouse models with fibroblast-specific knockout of PGC-1α. Results The expression of PGC-1α was induced in dermal fibroblasts of patients with SSc and experimental murine fibrosis. Transforming growth factor beta (TGFβ), hypoxia and epigenetic mechanisms regulate the expression of PGC-1α in fibroblasts. Knockdown of PGC-1α prevented the activation of autophagy by TGFβ and this translated into reduced fibroblast-to-myofibroblast differentiation and collagen release. Knockout of PGC-1α in fibroblasts prevented skin fibrosis induced by bleomycin and by overexpression of a constitutively active TGFβ receptor type I. Moreover, pharmacological inhibition of PGC-1α by SR18292 induced regression of pre-established, bleomycin-induced skin fibrosis. Conclusion PGC-1α is upregulated in SSc and promotes autophagy to foster TGFβ-induced fibroblast activation. Targeting of PGC-1α prevents aberrant autophagy, inhibits fibroblast activation and tissue fibrosis and may over therapeutic potential.}, author = {Zhang, Yun and Shen, Lichong and Zhu, Honglin and Dreissigacker, Katja and Distler, Diana and Zhou, Xiang and Györfi, Andrea-Hermina and Bergmann, Christina and Meng, Xianyi and Dees, Clara and Trinh-Minh, Thuong and Ludolph, Ingo and Horch, Raymund E. and Ramming, Andreas and Schett, Georg and Distler, Jörg}, doi = {10.1136/annrheumdis-2020-216963}, faupublication = {yes}, journal = {Annals of the Rheumatic Diseases}, keywords = {fibroblasts; systemic sclerosis; treatment}, note = {CRIS-Team Scopus Importer:2020-09-11}, pages = {1227-1233}, peerreviewed = {Yes}, title = {{PGC}-1α regulates autophagy to promote fibroblast activation and tissue fibrosis}, volume = {79}, year = {2020} } @article{faucris.107410864, abstract = {The Arterovenous Loop (AV Loop) model is a vascularization model in tissue engineering research, which is capable of generating a three dimensional in vivo unit with cells as well as the supporting vessels within an isolation chmaber. In our previous studies the AV loop in the isolation chamber was discovered to undergo hypoxia, characterized by Hypoxia Inducible Factor (HIF) up-regulation. The vascularization followed the increase of HIF-? temporally, while it was spatially positively correlated with the HIF-? level, as well. This study aims to prove that HIF-1a up-regulation is the stimulus for vascularization in the AV loop model.The AV loop model in rats was created by interposing a femoral vein graft into the distal ends of the contralateral femoral artery and vein, and the loop was embeded in fibrin matrix and fixed in isolation chamber. PHD (prolyl hydroxylases) inhibitor DMOG (Dimethyloxallyl Glycine) was applied systemically in the rats in 40 mg/KG at day 0 and day 3 (DMOG-1), or in 15 mg/KG at day 8, day10 and day12 (DMOG-2). Two weeks later the specimens were explanted and underwent morphological and molecular evaluations.Compared to the control group, in the DMOG-2 group the HIF-1? positive rate was siginicantly raised as shown in immunohistochemistry staining, accompanied with a smaller cross section area and greater vessel density, and a HIF-1? accumulation in the kidney. The mRNA of HIF-1? and its angiogenic target gene all increased in different extends. Ki67 IHC demostrate more positive cells. There were no significant change in the DMOG-1 group.By applying DMOG systemically, HIF-1? was up-regulated at the protein level and at the mRNA level, acompanied with angiogenic target gene up-regulateion, and the vascularization was promoted correspondingly. DMOG given at lower dosage constantly after one week tends to have better effect than the group given at larger dosage in the early stage in this model, and promotes cell proliferation, as evidenced by Ki67 IHC. Thus, this study proves that HIF-1a up-regulation is the stimulus for vascularization in the AV loop model and that the process of the vessel outgrowth can be controlled in the AV Loop model utilizing this mechanism.}, author = {Yuan, Quan and Bleiziffer, Oliver and Boos, Anja and Sun, Jiaming and Brandl, Anna and Beier, Justus and Arkudas, Andreas and Schmitz, Marweh and Kneser, Ulrich and Horch, Raymund E.}, doi = {10.1186/s12896-014-0112-x}, faupublication = {yes}, journal = {BMC Biotechnology}, note = {EVALuna2:15608}, pages = {112}, peerreviewed = {Yes}, title = {{PHDs} inhibitor {DMOG} promotes the vascularization process in the {AV} loop by {HIF}-1a up-regulation and the preliminary discussion on its kinetics in rat}, volume = {14}, year = {2014} } @article{faucris.119489964, abstract = {Phosphaturic mesenchymal tumor (PMT) is a rare neoplasm of uncertain histogenesis that has been linked to tumor-induced osteomalacia (TIO) since 1959. The neoplastic cells produce increased amount of FGF23 which results in TIO via uncontrolled renal loss of phosphate (phosphaturia), and consequently diminished bone mineralization. To date, ~300 cases have been reported. Although there is increasing evidence that PMT can be diagnosed by reproducible histopathologic features, firm diagnosis has been often restricted to cases associated with TIO and, hence, diagnosis of "nonphosphaturic variants" remained challenging. Recently, FGFR1/FN1 gene fusions were detected in roughly half of cases. We herein reviewed the clinicopathologic features of 22 PMTs (15 cases not published before), stained them with an extended immunohistochemical marker panel and examined them by fluorescence in situ hybridization for FGFR1 gene fusions. Patients were 12 males and 9 females (one of unknown sex) aged 33 to 83 years (median: 52 y). Lesions affected the soft tissues (n=11), bones (n=6), sinonasal tract (n=4), and unspecified site (n=1). Most lesions originated in the extremities (9 in the lower and 4 in the upper extremities). Acral sites were involved in 10 patients (6 foot/heel, 3 fingers/hands, and 1 in unspecified digit). Phosphaturia and TIO were recorded in 10/11 and 9/14 patients with detailed clinical data, respectively. Limited follow-up (5 mo to 14 y; median: 16 mo) was available for 14 patients. Local recurrence was noted in one patient and metastasis in another patient. Histologically, 11 tumors were purely of conventional mixed connective tissue type, 3 were chondromyxoid fibroma-like, 2 were hemangio-/glomangiopericytoma-like with giant cells, and 1 case each angiomyolipoma-like and reparative giant cell granuloma-like. Four tumors contained admixture of patterns (predominantly cellular with variable conventional component). Immunohistochemistry showed consistent expression of CD56 (11/11; 100%), ERG (19/21; 90%), SATB2 (19/21; 90%), and somatostatin receptor 2A (15/19; 79%), while other markers tested negative: DOG1 (0/17), beta-catenin (0/14), S100 protein (0/14), and STAT6 (0/7). FGFR1 fluorescence in situ hybridization was positive in 8/17 (47%) evaluable cases. These results add to the phenotypic delineation of PMT reporting for the first time consistent expression of SATB2 and excluding any phenotypic overlap with solitary fibrous tumor or sinonasal glomangiopericytoma. The unifying immunophenotype of the neoplastic cells irrespective of the histologic pattern suggests a specific disease entity with diverse morphotypes/variants rather than different neoplasms unified by TIO.}, author = {Agaimy, Abbas and Michal, Michael and Chiosea, Simion and Petersson, Fredrik and Hadravsky, Ladislav and Kristiansen, Glenn and Horch, Raymund E. and Schmolders, Jan and Hartmann, Arndt and Haller, Florian and Michal, Michal}, doi = {10.1097/PAS.0000000000000890}, faupublication = {yes}, journal = {American Journal of Surgical Pathology}, note = {EVALuna2:6834}, pages = {1371-1380}, peerreviewed = {Yes}, title = {{Phosphaturic} {Mesenchymal} {Tumors}: {Clinicopathologic}, {Immunohistochemical} and {Molecular} {Analysis} of 22 {Cases} {Expanding} their {Morphologic} and {Immunophenotypic} {Spectrum}}, volume = {41}, year = {2017} } @article{faucris.298207389, abstract = {Lipomatosis of peripheral nerves (LPN, also known as fibrolipomatous or lipofibromatous hamartoma of peripheral nerves) is a very rare, benign, intraneural, tumorous lesion that predominantly involves the median nerve but may rarely affect any peripheral nerve. Recently, PIK3CA mutations have been reported in macrodactyly, a rare condition related to LPN, and in other localized lipomatous overgrowth syndromes. In this retrospective study, we report 6 cases of FPN involving the median nerve (4 of them identified among 570 patients with carpal tunnel syndrome who underwent surgical decompression at our center from 2012 to 2022 and two seen in consultation by one of the authors). All cases were diagnosed via biopsy or resection supplemented by MRI. Patients were 4 males and 2 females aged 23 to 60 years (mean 38 years). One patient with bilateral lesions had in addition extensive angiomatosis of the paravertebral region. Histological examination showed an abnormal amount of mature fatty tissue containing disordered fibrous bands, entrapping normal-looking nerve fibers with prominent perineurial and endoneurial fibrosis. Genetic analysis using snapshot assay constructed to detect hotspots mutations in PIK3CA revealed similar PIK3CA mutations (p.H1047R; c.3140A>G) in 5/6 cases (83.3%). Our study represents a further contribution to the literature on LPN and highlights the diagnostic value of PIK3CA mutation testing as surrogate tool in equivocal cases and in those lesions without associated macrodactyly, especially as the biopsy findings of this lesion are essentially nonspecific.}, author = {Osterloh, Justus and Agaimy, Abbas and Fried, Frederick and Stöhr, Robert and Janka, Rolf Matthias and Arkudas, Andreas and Horch, Raymund E.}, doi = {10.1007/s00428-023-03540-7}, faupublication = {yes}, journal = {Virchows Archiv}, keywords = {Everolimus; Lipomatosis; mTOR; Neuropathy; Peripheral nerves; Sequencing}, note = {CRIS-Team Scopus Importer:2023-04-28}, peerreviewed = {Yes}, title = {{PIK3CA} mutation testing as a valuable molecular surrogate for lipomatosis of the median nerve: clinicopathological and molecular analysis of six cases}, year = {2023} } @article{faucris.211800443, abstract = {OBJECTIVES: The "angiosome" concept as a model for decision making in revascularisation of patients with critical limb ischaemia (CLI) has been subject to lively discussion in recent years. The aim of this prospective pilot study was to use intra-operative fluorescence angiography to provide further data on the angiosome concept on the level of microcirculation after tibial bypass surgery. DESIGN, MATERIALS, AND METHODS: This was a prospective analysis of 40 patients presenting with CLI Rutherford stage IV to VI before and after tibial bypass surgery. The macrocirculation was measured by the ankle brachial index. Skin microcirculation was assessed by intra-operative fluorescence angiography. The alteration of microcirculation was compared in direct and indirect revascularised angiosomes. Clinical follow-up investigations were performed and the wound healing rate was compared between the different revascularisation methods. RESULTS: Cumulated microcirculation parameters showed a significant improvement after surgery (ingress, ingress rate p<.001). Likewise, general microcirculatory improvement was observed in each foot angiosome after revascularisation, regardless of the tibial artery revascularised. Furthermore, a comparison of the direct (DR) and the indirect revascularised (IR) angiosomes did not show a significant difference concerning the improvement of microcirculation (difference DR-IR, ingress: 1.69, 95% CI 71.73-75.11; ingress rate: 0.08, 95% CI -12.91 to 13.07). The wound healing rate was similar in both groups, although the time to wound healing was faster by on average 2.5 months in the DR group (p=.083). CONCLUSION: Microcirculatory improvement was seen over the whole foot after tibial bypass. Therefore, fluorescence angiography is a promising tool to evaluate the angiosome concept in future larger studies. Clinicaltrials.gov: NCT03012750.}, author = {Rother, Ulrich and Lang, Werner and Horch, Raymund E. and Ludolph, Ingo and Meyer, Alexander and Gefeller, Olaf and Regus, Susanne}, doi = {10.1016/j.ejvs.2017.11.024}, faupublication = {yes}, journal = {European Journal of Vascular and Endovascular Surgery}, note = {EVALuna2:35687}, pages = {215-221}, peerreviewed = {Yes}, title = {{Pilot} {Assessment} of the {Angiosome} {Concept} by {Intra}-operative {Fluorescence} {Angiography} {After} {Tibial} {Bypass} {Surgery}}, volume = {55}, year = {2018} } @article{faucris.212496314, abstract = {Introduction: In this pilot study, we used indocyanine green fluorescence angiography during hemodialysis access surgery. The aim was to evaluate its relevance as a diagnostic tool to visualize changes in hand microperfusion. Patients and methods: In this prospective single-center study, 47 adult patients (33 male, 14 female) with renal disease (24 preemptive, 23 endstage) were enrolled. Surgical creation of an arteriovenous fistula was performed (22 forearm, 25 upper arm). Microperfusion of the ipsilateral hand and fingers was evaluated intraoperatively using indocyanine green fluorescence angiography. We compared the cumulated microperfusion parameters ingress (In) and ingress rate (InR) before and after opening of the anastomosis. To compare the dimension of microcirculatory decline, we calculated the ratios of the parameters (RatioIn and RatioInR) after to those before anastomosis opening. Results: The cumulated microperfusion parameters In and InR showed a significant decrease after completion of anastomosis and declamping. This effect has been seen in all patients for the hand and for each finger consecutively. During follow-up (mean 4.6, range 3-11 months), 5 patients (10.6%) complained about hemodialysis access-induced distal ischemia. The ratio of intraoperative microperfusion in those five hemodialysis access-induced ischemia patients was significantly lower compared to asymptomatic patients (RatioIn 0.23 vs 0.58, p = 0.001, and RatioInR 0.25 vs 0.62, p = 0.003). Conclusion: Intraoperative fluorescence angiography could visualize the deterioration of ipsilateral hand microperfusion after surgical creation of an arteriovenous fistula. It seems to be a promising tool to detect patients at risk for hemodialysis access-induced distal ischemia early in the peri- or even intraoperative stage.}, author = {Regus, Susanne and Klingler, Felix and Lang, Werner and Meyer, Alexander and Almási-Sperling, Veronika and May, Matthias and Wüst, Wolfgang and Rother, Ulrich}, doi = {10.1177/1129729818791989}, faupublication = {yes}, journal = {Journal of Vascular Access}, note = {CRIS-Team WoS Importer:2019-03-06}, pages = {175-183}, peerreviewed = {Yes}, title = {{Pilot} study using intraoperative fluorescence angiography during arteriovenous hemodialysis access surgery}, volume = {20}, year = {2019} } @article{faucris.109433104, abstract = {Defects of the perineum may result from ablative procedures of different malignancies. The evolution of more radical excisional surgery techniques resulted in an increase in large defects of the perineum. The perineogenital region per se has many different functions for urination, bowel evacuation, sexuality, and reproduction. Up-to-date individual and interdisciplinary surgical treatment concepts are necessary to provide optimum oncological as well as quality of life outcome. Not only the reconstructive method but also the timing of the reconstruction is crucial. In cases of postresectional exposition of e.g., pelvic or femoral vessels or intrapelvic and intra-abdominal organs, simultaneous flap procedure is mandatory. In particular, the reconstructive armamentarium of the plastic surgeon should include not only pedicled flaps but also free microsurgical flaps so that no compromise in terms of the extent of the oncological resection has to be accepted. For intra-abdominally and/or pelvic tumors of the rectum, the anus, or the female reproductive system, which were resected through an abdominally and a sacrally surgical access, simultaneous vertical rectus abdominis myocutaneous (VRAM) flap reconstruction is recommendable. In terms of soft tissue sarcoma of the pelvic/caudal abdomen/proximal thigh region, two-stage reconstructions are possible. This review focuses on the treatment of perineum, genitals, and pelvic floor defects after resection of malignant tumors, giving a distinct overview of the different types of defects faced in this region and describing a number of reconstructive techniques, especially VRAM flap and pedicled flaps like antero-lateral thigh flap or free flaps. Finally, this review outlines some considerations concerning timing of the different operative steps.}, author = {Brodbeck, Rebekka and Horch, Raymund E. and Arkudas, Andreas and Beier, Justus}, doi = {10.3389/fonc.2015.00212}, faupublication = {yes}, journal = {Frontiers in Oncology}, note = {EVALuna2:15599}, pages = {212}, peerreviewed = {Yes}, title = {{Plastic} and {Reconstructive} {Surgery} in the {Treatment} of {Oncological} {Perineal} and {Genital} {Defects}}, volume = {5}, year = {2015} } @article{faucris.223989301, abstract = {Due to the increasing clinical application of adipose-derived stem cells (ADSC), e.g. lipotransfer for breast reconstruction, this study aimed to gain novel insights regarding ADSC influence on breast tissue remodeling and determine patient-dependent factors affecting lipotransfer as well as begin to address its oncological risks. The ADSC secretome was analyzed from five normal breast reduction patients and contained elevated levels of growth factors, cytokines and proteins mediating invasion. ADSC/ADSC secretomes were tested for their influence on the function of primary mammary epithelial cells, and tumor epithelial cells using cell culture assays. ADSC/ADSC secretomes significantly stimulated proliferation, transmigration and 3D-invasion of primary normal and tumor epithelial cells. IL-6 significantly induced an EMT and invasion. The ADSC secretome significantly upregulated normal epithelial cell gene expression including MMPs and ECM receptors. Our study supports that ADSC and its secretome promote favorable conditions for normal breast tissue remodeling by changing the microenvironment. and may also be important regarding residual breast cancer cells following surgery.}, author = {Kengelbach-Weigand, Annika and Tasbihi, Kereshmeh and Strissel, Pamela and Schmid, Rafael and Marques, Jasmin Monteiro and Beier, Justus and Beckmann, Matthias and Strick, Reiner and Horch, Raymund E. and Boos, Anja}, doi = {10.1038/s41598-019-47224-2}, faupublication = {yes}, journal = {Scientific Reports}, note = {CRIS-Team Scopus Importer:2019-08-06}, peerreviewed = {Yes}, title = {{Plasticity} of patient-matched normal mammary epithelial cells is dependent on autologous adipose-derived stem cells}, volume = {9}, year = {2019} } @article{faucris.234227477, abstract = {During the last two decades, understanding and experience of microsurgical reconstruction have increased. This is linked to improved instruments and technical devices, as well as to the increased number of available donor sites for microvascular flap transfer. If the indication is appropriate, it is now possible to perform personalised, safe and successful microsurgical reconstructions, almost independently of the patients' age. Despite these constant developments, there is little specific literature on microsurgery related to the specific problems of the elderly patient. During two consensus workshops organised by the DAM (German Speaking Working Group for Microsurgery of the Peripheral Nerves and Vessels), the latest findings and experience with microsurgical reconstruction in the elderly patient were presented by a group of experts and current recommendations were formulated. On the basis of these consensus workshops and the current literature, this article discusses important issues in this area. }, author = {Ludolph, Ingo and Lehnhardt, Marcus and Arkudas, Andreas and Kneser, Ulrich and Pierer, Gerhard and Harder, Yves and Horch, Raymund E.}, doi = {10.1055/s-0043-115730}, faupublication = {yes}, journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie}, note = {EVALuna2:211479}, pages = {118-125}, peerreviewed = {Yes}, title = {{Plastic} reconstructive microsurgery in the elderly patient - {Consensus} statement of the {German} {Speaking} {Working} {Group} for {Microsurgery} of the {Peripheral} {Nerves} and {Vessels}}, volume = {50}, year = {2018} } @article{faucris.245470688, abstract = {Due to the increasing further development of oncological surgical procedures, in particular extralevatory rectal extirpation in combination with neoadjuvant radiation of the pelvis, the risk of local recurrence of rectal cancer (LRRC) could be reduced to 5–10%. Curative treatment of LRRC necessitates a complete resection with microscopically tumor-free borders (R0); however, the oncologically meaningful radical surgery frequently leads to substantial structural and functional pelvic floor and perineal defects. The resulting mostly irradiated wound cavity after abdominoperineal extirpation (APE) or pelvic exenteration has a high risk of wound healing disorders after primary closure. For primary and also recurrent rectal and anal cancer it was shown that interdisciplinary one-stage reconstructive plastic surgery of the pelvic floor and the perineum and also the vagina, e.g. with a transpelvic vertical rectus abdominis myocutaneous (VRAM) flap, can improve the quality of life and reduce hospitalization times. Even in the long-term course, protracted secondary wound healing disorders can be minimized or avoided using this method.}, author = {Horch, Raymund E. and Arkudas, Andreas and Ludolph, Ingo and Cai, Aijia and Mulica, Markus}, doi = {10.1007/s00053-020-00498-x}, faupublication = {yes}, journal = {Coloproctology}, keywords = {Interdisciplinary surgery; Pelvic exenteration; Pelvic floor reconstruction; Rectal cancer; VRAM-flap}, note = {CRIS-Team Scopus Importer:2020-11-20}, peerreviewed = {No}, title = {{Plastisch}-chirurgische {Rekonstruktion} bei ausgedehnten {Beckenbodendefekten} nach onkologischer {Chirurgie}}, year = {2020} } @article{faucris.243610887, abstract = {The treatment of leg ulcers requires a comprehensive assessment of the patient and the underlying pathogenesis and is subject of multimodal concepts. After unsuccessful conservative treatments, the surgical wound debridement is the treatment of choice. This is complemented by local vacuum therapy or in case of chronic infected wounds by applying an instillation therapy. After adequate wound conditioning and need for plastic surgical reconstruction, minimally invasive procedures such as skin transplantation in superficial defects, up to microsurgical tissue transplantation in severe losses of substance are available. If a perfusion disorder of the affected limb is presented, a revascularisation by bypasses or AV-loops is required. With new methods in wound healing, the success rate and therefore also the quality of life of patients with chronic wounds may be improved.}, author = {Boos, Anja and Hillenbrand, M. and Beier, Justus and Arkudas, Andreas and Meyer, Alexander and Lang, Werner and Horch, Raymund E.}, faupublication = {yes}, journal = {Internistische Praxis}, keywords = {Av-loop; Bypass; Free flap; Leg ulcers; Surgical therapy}, note = {CRIS-Team Scopus Importer:2020-10-09}, pages = {641-649}, peerreviewed = {No}, title = {{Plastisch}-chirurgische {Rekonstruktionsverfahren} des {Ulcus} {Cruris}}, volume = {62}, year = {2020} } @article{faucris.204373256, abstract = {OBJECTIVES: The enzyme poly(ADP-ribose) polymerase-1 (PARP-1) transfers negatively charged ADP-ribose units to target proteins. This modification can have pronounced regulatory effects on target proteins. Recent studies showed that PARP-1 can poly(ADP-ribosyl)ate (PARylate) Smad proteins. However, the role of PARP-1 in the pathogenesis of systemic sclerosis (SSc) has not been investigated. METHODS: The expression of PARP-1 was determined by quantitative PCR and immunohistochemistry. DNA methylation was analysed by methylated DNA immunoprecipitation assays. Transforming growth factor-β (TGFβ) signalling was assessed using reporter assays, chromatin immunoprecipitation assays and target gene analysis. The effect of PARP-1 inactivation was investigated in bleomycin-induced and topoisomerase-induced fibrosis as well as in tight-skin-1 (Tsk-1) mice. RESULTS: The expression of PARP-1 was decreased in patients with SSc, particularly in fibroblasts. The promoter of PARP-1 was hypermethylated in SSc fibroblasts and in TGFβ-stimulated normal fibroblasts. Inhibition of DNA methyltransferases (DNMTs) reduced the promoter methylation and reactivated the expression of PARP-1. Inactivation of PARP-1 promoted accumulation of phosphorylated Smad3, enhanced Smad-dependent transcription and upregulated the expression of TGFβ/Smad target genes. Inhibition of PARP-1 enhanced the effect of TGFβ on collagen release and myofibroblast differentiation in vitro and exacerbated experimental fibrosis in vivo. PARP-1 deficiency induced a more severe fibrotic response to bleomycin with increased dermal thickening, hydroxyproline content and myofibroblast counts. Inhibition of PARylation also exacerbated fibrosis in Tsk-1 mice and in mice with topoisomerase-induced fibrosis. CONCLUSION: PARP-1 negatively regulates canonical TGFβ signalling in experimental skin fibrosis. The downregulation of PARP-1 in SSc fibroblasts may thus directly contribute to hyperactive TGFβ signalling and to persistent fibroblast activation in SSc.}, author = {Zhang, Yun and Pötter, Sebastian and Chen, Chih-Wei and Liang, Ruifang and Gelse, Kolja and Ludolph, Ingo and Horch, Raymund E. and Distler, Oliver and Schett, Georg and Distler, Jörg and Dees, Clara}, doi = {10.1136/annrheumdis-2017-212265}, faupublication = {yes}, journal = {Annals of the Rheumatic Diseases}, note = {EVALuna2:34150}, pages = {744-751}, peerreviewed = {Yes}, title = {{Poly}({ADP}-ribose) polymerase-1 regulates fibroblast activation in systemic sclerosis}, volume = {77}, year = {2018} } @article{faucris.285403613, abstract = {With the evolution of suture materials, there has been a change in paradigms in primary and secondary tendon repair. Improved mechanical properties allow more aggressive rehabilitation and earlier recovery. However, for the repair to hold against higher mechanical demands, more advanced suturing and knotting techniques must be assessed in combination with those materials. In this protocol, the use of polytetrafluoroethylene (PTFE) as a suture material in combination with different repair techniques was investigated. In the first part of the protocol, both linear tension strength and elongation of knotted against not-knotted strands of three different materials used in flexor tendon repair were evaluated. The three different materials are polypropylene (PPL), ultra-high molecular weight polyethylene with a braided jacket of polyester (UHMWPE), and polytetrafluoroethylene (PTFE). In the next part (ex vivo experiments with cadaveric flexor tendons), the behavior of PTFE using different suture techniques was assessed and compared with PPL and UHMWPE. This experiment is comprised of four steps: harvesting of the flexor tendons from fresh cadaveric hands, transection of the tendons in a standardized manner, tendon repair by four different techniques, mounting, and measurement of the tendon repairs on a standard linear dynamometer. The UHMWPE and PTFE showed comparable mechanical properties and were significantly superior to PPL in terms of linear traction strength. Repairs with four- and six-strand techniques proved stronger than two-strand techniques. Handling and knotting of PTFE are a challenge due to very low surface friction but fastening of the four- or six-strand repair is comparatively easy to achieve. Surgeons routinely use PTFE suture material in cardiovascular surgery and breast surgery. The PTFE strands are suitable for use in tendon surgery, providing a robust tendon repair so that early active motion regimens for rehabilitation can be applied.}, author = {Polykandriotis, Elias and Himmler, Marcus and Mansouri, Shirin and Ruppe, Florian and Grüner, Jasmin and Bräuer, Lars and Schubert, Dirk W. and Horch, Raymund E.}, doi = {10.3791/64115}, faupublication = {yes}, journal = {Journal of Visualized Experiments}, note = {CRIS-Team Scopus Importer:2022-11-18}, peerreviewed = {Yes}, title = {{Polytetrafluoroethylene} ({PTFE}) as a {Suture} {Material} in {Tendon} {Surgery}}, year = {2022} } @article{faucris.257707168, abstract = {Background In this study, we evaluate the value of novel suture material based on monofilamentous-extruded polyfluoroethylene (PTFE) compared to polypropylene (PPL) and Fiberwire (FW). Materials and methods 60 flexor tendons were harvested from fresh cadaveric upper extremities. 4-0 sutures strands were used in the PPL, FW and PTFE group. Knotting properties and mechanical characteristics of the suture materials were evaluated. A 4-strand locked cruciate (Adelaide) or a 6-strand (M-Tang) suture technique was applied as core sutures for a tendon repair. Two-way ANOVA tests were performed with the Bonferroni correction. Results Stable knotting was achieved with 5 throws with the PPL material, 7 throws for FW and 9 throws for PTFE. In the PPL group, linear tensile strength was 45.92 +/- 12.53 N, in the FW group 80.11 +/- 18.34 N and in the PTFE group 76.16 +/- 29.10 N. FW and PTFE are significantly stronger than PPL but show no significant difference among each other. Similar results were obtained in the subgroup comparisons for different repair techniques. The Adelaide and the M-Tang knotting technique showed no significant difference. Conclusion Fiberwire showed superior handling and knotting properties in comparison to PTFE. However, PTFE allows easier approximation of the stumps. In both, M-Tang and Adelaide repairs, PTFE was equal to FW in terms of repair strength. Both PTFE and FW provide for a robust tendon repair so that early active motion regimens for rehabilitation can be applied.}, author = {Polykandriotis, Elias and Ruppe, Florian and Niederkorn, Miriam and Polykandriotis, Ektor and Bräuer, Lars and Horch, Raymund E. and Arkudas, Andreas and Grüner, Johanna}, doi = {10.1007/s00402-021-03899-9}, faupublication = {yes}, journal = {Archives of Orthopaedic and Trauma Surgery}, note = {CRIS-Team WoS Importer:2021-05-07}, peerreviewed = {Yes}, title = {{Polytetrafluoroethylene} ({PTFE}) suture vs fiberwire and polypropylene in flexor tendon repair}, year = {2021} } @article{faucris.210313922, abstract = {Endothelial progenitor cells (EPCs) contribute to neovascularization in tumors. However, the relationship of EPCs and tumor-induced angiogenesis still remains to be clarified. The present study aimed at investigating the influence of 4 different tumor types on angiogenic properties of EPCs in an in vitro and in vivo rat model. It could be demonstrated that in vitro proliferation, migration, and angiogenic abilities and genetic modifications of EPCs are controlled in a tumor-type-dependent manner. The proangiogenic effect of mammary carcinoma, osteosarcoma, and rhabdomyosarcoma cells was more pronounced compared to colon carcinoma cells. Coinjection of encapsulated tumor cells, especially mammary carcinoma cells, and EPCs in a rat model confirmed a contributing effect of EPCs in tumor vascularization. Cytokines secreted by tumors such as monocyte chemoattractant protein 1, macrophage inflammatory protein 2, and TNF-related apoptosis-inducing ligand play a pivotal role in the tumor cell-EPC interaction, leading to enhanced migration and angiogenesis. With the present study, we were able to decipher possible underlying mechanisms by which EPCs are stimulated by tumor cells and contribute to tumor vascularization. The present study will contribute to a better understanding of tumor-induced vascularization, thus facilitating the development of therapeutic strategies targeting tumor-EPC interactions.-An, R., Schmid, R., Klausing, A., Robering, J. W., Weber, M., Bäuerle, T., Detsch, R., Boccaccini, A. R., Horch, R. E., Boos, A. M., Weigand, A. Proangiogenic effects of tumor cells on endothelial progenitor cells vary with tumor type in an in vitro and in vivo rat model.}, author = {An, Ran and Schmid, Rafael and Klausing, Anne and Robering, Jan Willem and Weber, Manuel and Bäuerle, Tobias and Detsch, Rainer and Boccaccini, Aldo R. and Horch, Raymund E. and Boos, Anja and Weigand, Annika}, doi = {10.1096/fj.201800135RR}, faupublication = {yes}, journal = {The FASEB Journal}, pages = {5587-5601}, peerreviewed = {Yes}, title = {{Proangiogenic} effects of tumor cells on endothelial progenitor cells vary with tumor type in an in vitro and in vivo rat model.}, volume = {32}, year = {2018} } @inproceedings{faucris.113904164, author = {Horch, Raymund E. and Arkudas, Andreas and Schmitz, Marweh and Beier, Justus and Schultz, Gregory and Schubert, Dirk W.}, booktitle = {14th BIONANO-MATERIALS}, faupublication = {yes}, peerreviewed = {unknown}, title = {{Problematik} der biofilm-{Besiedlung} von {Biomaterialien} bei der {Translation} in den klinischen {Einsatz} am {Beispiel} von {Brustimplantaten}}, venue = {Erlangen}, year = {2013} } @article{faucris.285804893, abstract = {Distinct sensory properties of unmyelinated axons in the isolated rat sciatic nerve have previously been revealed by measuring stimulated neuropeptide (CGRP) release in response to noxious stimuli. Axonal sensitization to heat by inflammatory mediators has been demonstrated and shown to depend on the heat- and proton-activated ion channel TRPV1. Recently, we have demonstrated in vitro that heat stimulation of nociceptive axons generates ectopic action potential discharge which resembles the heat response of the corresponding cutaneous nerve endings. It remained however, to be established whether adequate axonal stimulation could also generate projected sensations in a conscious human subject. In a singular human trial, the superficial radial nerve (SR) was exposed and stimulated mechanically as well as with noxious cold (3 °C). These stimuli were unable to induce any conscious local or projected sensations. However, controlled radiant heat applied to the nerve resulted in intense slowly adapting burning pain sensations projected into the center of the SR innervation area. No local sensation was reported. Thus, presumably activated nervi nevorum in the sheath of a healthy nerve do not cause conscious sensations, while axons of passage in mid-nerve exhibit a sensory transduction capacity for noxious heat though not for mechanical and cold stimulation. Axonal heat transduction may therefore become a source of ectopic discharge and neuropathic pain when heat threshold drops to body temperature as is the case with peripheral nerve endings in inflamed skin. © 2008 European Federation of Chapters of the International Association for the Study of Pain.}, author = {Hoffmann, Tal and Sauer, Susanne and Horch, Raymund E. and Reeh, Peter}, doi = {10.1016/j.ejpain.2008.09.014}, faupublication = {yes}, journal = {European Journal of Pain}, keywords = {Nervi nervorum; Neuropathy; Nociception; Sensory transduction}, note = {CRIS-Team Scopus Importer:2022-11-28}, pages = {35-37}, peerreviewed = {Yes}, title = {{Projected} pain from noxious heat stimulation of an exposed peripheral nerve - {A} case report}, volume = {13}, year = {2009} } @inproceedings{faucris.266101669, abstract = {Nowadays, many surgical procedures require preoperative planning, mostly relying on data from 3D imaging techniques like computed tomography or magnetic resonance imaging. However, preoperative assessment of this data is carried out on the PC (using classical CT/MR viewing software) and not on the patient's body itself. Therefore, surgeons need to transfer both their overall understanding of the patient's individual anatomy and also specific markers and labels for important points from the PC to the patient only with the help of imaginative power or approximative measurement. In order to close the gap between preoperative planning on the PC and surgery on the patient, we propose a system to directly project preoperative knowledge to the body surface by projection mapping. As a result, we are able to display both assigned labels and a volumetric and view-dependent view of the 3D data in-situ. Furthermore, we offer a method to interactively navigate through the data and add 3D markers directly in the projected volumetric view. We demonstrate the benefits of our approach using DIEP flap breast reconstruction as an example. By means of a small pilot study, we show that our method outperforms standard surgical planning in accuracy and can easily be understood and utilized even by persons without any medical knowledge.}, author = {Martschinke, Jana and Klein, Vanessa and Kurth, Philipp and Engel, Klaus and Ludolph, Ingo and Hauck, Theresa and Horch, Raymund E. and Stamminger, Marc}, booktitle = {Eurographics Workshop on Visual Computing for Biology and Medicine}, doi = {10.2312/vcbm.20211354}, editor = {Oeltze-Jafra, Steffen and Smit, Noeska N. and Sommer, Björn and Nieselt, Kay and Schultz, Thomas}, faupublication = {yes}, isbn = {978-3-03868-140-3}, peerreviewed = {Yes}, publisher = {Eurographics}, title = {{Projection} {Mapping} for {In}-{Situ} {Surgery} {Planning} by the {Example} of {DIEP} {Flap} {Breast} {Reconstruction}}, url = {https://www.lgdv.tf.fau.de/?p=2193}, year = {2021} } @article{faucris.310442356, abstract = {Background: Carpal tunnel syndrome, A1 annular pulley stenosis and Dupuytren’s contracture are among the most common conditions of the hand. In this study, we investigated the impact of surgical procedure on hand grip strength and high-resolution spatial load distribution in individuals suffering from those diseases over a follow-up period of one year. Materials and methods: In this prospective study, data of 9 patients with carpal tunnel syndrome, 12 patients with A1 annular pulley stenosis and 7 patients with Dupuytren’s contracture were evaluated. Only patients with unilateral disease were included providing the contralateral hand as an intra-individual control. Grip strength was measured with cylindrical instruments in two different sizes with respect to the hand size of the patients. Maximum and average values of grip strength as well as spatial load distribution in each finger, thenar, hypothenar and palm were analyzed. Data of the affected patients were collected preoperatively and 6 weeks, 6 months and 1 year postoperatively. Grip strength and spatial load distribution were compared preoperatively to postoperatively. In addition, DASH score, Levine score, 2-point discrimination and degree of flexion contracture were assessed. Results: The patients with A1 annular pulley stenosis showed a significant increase in grip strength 6 months and one year postoperatively. Patients with carpal tunnel syndrome and Dupuytren’s contracture showed no significant difference in grip strength over the course of time. An increase in the percentual grip strength of the thenar in patients with carpal tunnel disease and within the affected finger in A1 annular pulley stenosis was observed over the course of time. The DASH score was significantly lower in all patient cohorts one year postoperatively. Conclusion: Surgical procedure in carpal tunnel syndrome, A1 annular ligament stenosis and Dupuytren’s contracture improves the functionality of the hand in everyday life. Some areas of the hand seem to compensate other weaker areas in grip strength.}, author = {Grüner, Jasmin and Cai, Aijia and Pingel, Isabel and Horch, Raymund E. and Beier, Justus and Arkudas, Andreas}, doi = {10.1007/s00402-023-04984-x}, faupublication = {yes}, journal = {Archives of Orthopaedic and Trauma Surgery}, keywords = {A1 annular pulley stenosis; Carpal tunnel syndrome; Cylinder grip; Dupuytren’s contracture; Grip strength; Manugraphy}, note = {CRIS-Team Scopus Importer:2023-09-15}, pages = {6477-6485}, peerreviewed = {Yes}, title = {{Prospective} analysis of grip strength and load distribution after surgical treatment of common diseases of the hand with novel’s manugraphy® system}, volume = {143}, year = {2023} } @article{faucris.237478868, abstract = {BACKGROUND: The loss of a breast, and thus of the female body shape, is often extremely traumatising for women affected. Although free flap grafts have become the gold standard in reconstructive breast surgery, it has not been possible to date to unequivocally document their superiority over conventional alternative techniques. To date, there are no data on the care situation in Germany. Neither the number of reconstructions nor the proportion of the individual techniques is known. A prospective online registry has been set up in order to systematically collect and transparently present the structure and quality of care. The long-term goals are to record the existing quality of care, improve deficits and to certify centres with the appropriate expertise to establish a high level of care nationwide. METHODS: For this purpose, the German Society of Plastic, Reconstructive and Aesthetic Surgeons (DGPRÄC) has set up an internet-based database in which treatment data are recorded anonymously. As part of a certification process, auditors checked structure quality, compliance with specifications, and the stringency of the data entered in comparison with the hospital's internal documentation. If the evaluation is positive, the DGPRÄC quality seal is awarded. RESULTS: Since its introduction in 2012, the database has provided validated information. Initially, only a few departments were prepared to present their results transparently. However, the number has grown steadily in recent years. As a result, the quality recording assumed a pioneering function nationwide and can now be used as a reliable source of information by patients and doctors. The DGPRÄC website features a map of the centres, which are colour-coded according to their respective qualifications. It is thus possible to find a qualified plastic surgeon with comprehensive expertise in acceptable proximity to every oncological breast centre. CONCLUSION: The database provides an overview of the quality of the various surgical techniques and thus the possibility of serious self monitoring and scientific analysis. The systematic collection of data has contributed to strengthening the position of plastic surgery in the field of reconstructive breast surgery. However, the financial and personnel resources required to establish the database were clearly underestimated.}, author = {Fritschen, Uwe von and Grill, Barbara and Wagner, Juri and Schuster, Horst and Sukhova, Inesa and Giunta, Riccardo E. and Heitmann, Christoph and Andree, Christoph and Horch, Raymund E. and Kneser, Ulrich and Germann, Günter}, doi = {10.1055/a-1075-2525}, faupublication = {yes}, journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie}, note = {CRIS-Team Scopus Importer:2020-04-17}, pages = {58-66}, peerreviewed = {Yes}, title = {{Qualitätssicherung} in der {Brustrekonstruktion} – {Etablierung} eines prospektiven nationalen {Onlineregisters} für mikrochirurgische {Brustrekonstruktionen}}, volume = {52}, year = {2020} } @article{faucris.210675588, abstract = {OBJECTIVE: Indocyanine green fluorescence angiography enables a quantitative real-time perfusion assessment in kidney transplantation. The results of intraoperative microperfusion of the kidney allograft were compared to the renal chronicity score in pre-transplantation kidney biopsy specimens. The intrarenal resistance index was calculated by duplex sonography as a method of reference. METHODS: Seventy-seven patients with end stage renal disease undergoing kidney transplantation were prospectively included in two centers. Correlation analysis of chronic changes in kidney biopsy specimens and the ingress of ICG fluorescence signal were investigated. RESULTS: The results yielded a significantly negative correlation for the renal chronicity (r= -0.294, p=0.017) as well as the intestinal fibrosis and tubular atrophy score (r=-0.328, p=0.007). There was a significant inverse relationship between the ingress and the mean RI values of the upper pole of the kidney allograft. CONCLUSION: In summary, fluorescence angiography reflects preexisting morphological changes of the renal cortex. ICG angiography may serve as an alternative method for the assessment of microperfusion of the kidney allograft. This article is protected by copyright. All rights reserved.}, author = {Rother, Ulrich and Amann, Kerstin Ute and Adler, Werner and Nawroth, Nina and Karampinis, Ioannis and Keese, Michael and Manap, Serhat and Regus, Susanne and Meyer, Alexander and Porubsky, Stefan and Hilgers, Karl Friedrich and Krämer, Bernhard K. and Lang, Werner and Nowak, Kai and Gerken, Andreas L.H.}, doi = {10.1111/micc.12529}, faupublication = {yes}, journal = {Microcirculation}, note = {EVALuna2:35427}, peerreviewed = {Yes}, title = {{Quantitative} assessment of microperfusion by indocyanine green angiography in kidney transplantation resembles chronic morphological changes in kidney specimens}, year = {2019} } @article{faucris.122733644, abstract = {(23)Na magnetic resonance imaging ((23)Na-MRI) is able to measure Na(+) in vivo in humans and allows quantification of tissue sodium distribution. We now tested the utility of (23)Na-MRI technique in detecting and assessing sports-related acute muscular injury.We assessed tissue Na(+) of both lower legs with a 3T MRI scanner using a customized (23)Na knee coil. The affected left calf muscle in an injured volleyball player showed a hyperintense Na(+) signal. Follow-up measurements revealed persistently increased muscle Na(+) content despite complete clinical recovery.Our findings suggest that (23)Na-MRI could have utility in detecting subtle muscular injury and might indicate when complete healing has occurred. Furthermore, (23)Na-MRI suggests the presence of substantial injury-related muscle electrolyte shifts that warrant more detailed investigation.}, author = {Dahlmann, Anke and Kopp, Christoph and Linz, Peter and Seuß, Hannes and Eckardt, Kai-Uwe and Luft, Friedrich C. and Titze, Jens and Uder, Michael and Hammon, Matthias and Cavallaro, Alexander Josef}, doi = {10.1186/s40064-016-2193-6}, faupublication = {yes}, journal = {SpringerPlus}, note = {EVALuna2:3865}, pages = {661}, peerreviewed = {Yes}, title = {{Quantitative} assessment of muscle injury by (23){Na} magnetic resonance imaging}, volume = {5}, year = {2016} } @article{faucris.204501155, abstract = {Plastic surgery is extremely diverse and offers various fields of work. To provide optimal support for plastic surgeons in training, it is therefore paramount to know their mid- and long-term goals. To that end, we conducted a web-based survey among the 462 associated members of the German Association of Plastic, Reconstructive and Aesthetic Surgeons (DGPRÄC). Besides asking questions about the current status, we specifically inquired about the pursued setting and main area of work. 84 completed questionnaires were evaluated. Most respondents saw their future in a private practice setting. Reconstructive surgery and aesthetic surgery were by far the most sought-after fields, while only a few of the associated members pursued a career in hand surgery, and none of them wished to work in burn care. At the same time, they felt that aesthetic surgery was the field where training was most insufficient. Therefore, measures need to be taken to improve the training in aesthetic surgery. Also, it is paramount to increase the attractiveness of both the fields of hand surgery and burn care to assure highly qualified care in the future.}, author = {Kolbenschlag, Jonas and Paprottka, Felix and Wagner, Juri and Goertz, Ole and Liebau, Jutta and Daigeler, Adrien and Horch, Raymund E. and Horch, Raymund E. and Lehnhardt, Marcus}, doi = {10.1055/s-0043-108430}, faupublication = {yes}, journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie}, note = {EVALuna2:32839}, pages = {267-272}, peerreviewed = {Yes}, title = {{Quo} vadis {DGPRÄC}: associated members' goals for the future}, volume = {49}, year = {2017} } @article{faucris.255615861, abstract = {INTRODUCTION: In reconstructive surgery, the latissimus dorsi (LD) muscle is known as a workhorse flap and is commonly used as a pedicled or free flap. Postoperative complications of a reconstructive procedure with an LD flap include seroma and hematoma formation at an early stage after LD transfer. Late-onset hematoma at the donor site are considered to be extremely rare postoperative complications; late, expanding pseudotumor-like hematoma can occur months or years after surgery. Shearing forces and poor coagulation are thought to be the primary reasons for these postoperative complications. CASE REPORTS: This report presents 2 cases of pseudotumors 12 and 29 years after LD transfer. Magnetic resonance imaging was performed prior to complete surgical excision. After surgical removal, patients received negative-pressure wound therapy with instillation and dwell time (NPWTi-d) for several days. After vacuum-assisted wound bed preparation, wound closure was performed with secondary sutures. CONCLUSIONS: The case report presented 2 incidences of rare late-onset pseudotumors many years after the initial LD reconstruction. To the authors' knowledge, this late-onset occurrence (ie, after 3 decades) has not been reported in the literature to date. If solidification of the late hematoma makes aspiration impossible, surgical intervention is required. Negative pressure wound therapy with instillation and dwell time potentially minimizes the wound size and reduces shear forces at the back donor-site.}, author = {Stumpfe, Maximilian C. and Horch, Raymund E. and Geierlehner, Alexander and Ludolph, Ingo}, faupublication = {yes}, journal = {Wounds: A Compendium of Clinical Research and Practice}, note = {CRIS-Team Scopus Importer:2021-04-19}, pages = {E101-E105}, peerreviewed = {Yes}, title = {{Rare} {Pseudotumor}-like {Hematoma} at the {Latissimus} {Dorsi} {Muscle} {Flap} {Donor} {Site}: {A} {Treatment} {Strategy} {Utilizing} {Negative} {Pressure} {Wound} {Therapy} {With} {Instillation} and {Dwell} {Time}}, volume = {32}, year = {2020} } @article{faucris.282426972, abstract = {Targeted therapies using biopharmaceuticals are of growing clinical importance in disease treatment. Currently, there are several limitations of protein-based therapeutics (biologicals), including suboptimal biodistribution, lack of stability, and systemic side effects. A promising approach to overcoming these limitations could be a therapeutic cell-loaded 3D construct consisting of a suitable matrix component that harbors producer cells continuously secreting the biological of interest. Here, the recombinant spider silk proteins eADF4(C16), eADF4(C16)-RGD, and eADF4(C16)-RGE have been processed together with HEK293 producer cells stably secreting the highly traceable reporter biological TNFR2-Fc-GpL, a fusion protein consisting of the extracellular domain of TNFR2, the Fc domain of human IgG1, and the luciferase of Gaussia princeps as a reporter domain. eADF4(C16) and eADF4(C16)-RGD hydrogels provide structural and mechanical support, promote HEK293 cell growth, and allow fusion protein production by the latter. Bioink-captured HEK293 producer cells continuously release functional TNFR2-Fc-GpL over 14 days. Thus, the combination of biocompatible, printable spider silk bioinks with drug-producing cells is promising for generating implantable 3D constructs for continuous targeted therapy.}, author = {Trossmann, Vanessa T. and Heltmann-Meyer, Stefanie and Amouei, Hanna and Wajant, Harald and Horch, Raymund E. and Steiner, Dominik and Scheibel, Thomas}, doi = {10.1021/acs.biomac.2c00971}, faupublication = {yes}, journal = {Biomacromolecules}, note = {CRIS-Team Scopus Importer:2022-09-30}, peerreviewed = {Yes}, title = {{Recombinant} {Spider} {Silk} {Bioinks} for {Continuous} {Protein} {Release} by {Encapsulated} {Producer} {Cells}}, year = {2022} } @article{faucris.234121776, abstract = {BACKGROUND: Reconstruction of cranial composite defects, including all layers of the scalp and the neurocranium, poses an interdisciplinary challenge. Especially after multiple previous operations and/or radiation therapy, sufficient reconstruction is often only possible using microsurgical free flap transplantation. The aim of this study was to analyze the therapy of interdisciplinary cases with composite defects including the scalp and neurocranium. METHODS: From 2009 to 2017, 23 patients with 18 free flaps and 10 pedicled/local flaps were analyzed. First choices for free flaps were muscle flaps followed by fasciocutaneous flaps. RESULTS: Except for four patients, a stable coverage could be reached in the first operation. Three of these patients received a local scalp rotation flap in the first operation and needed an additional free flap because the local flap was no longer sufficient for coverage after wound healing deficiency or tumor relapse. The superficial temporal artery or external carotid artery served as recipient vessels. In special cases, venous grafts or an arteriovenous loop (AV loop) were used as extensions for the recipient vessels. CONCLUSIONS: In summary, an interdisciplinary approach with radical debridement of infected or necrotic tissue and the reconstruction of the dura mater are essential to reach a stable, long-lasting reconstructive result. Based on our experience, free flaps seem to be the first choice for patients after multiple previous operations and/or radiation therapy.}, author = {Steiner, Dominik and Horch, Raymund E. and Eyüpoglu, Ilker Yasin and Buchfelder, Michael and Arkudas, Andreas and Schmitz, Marweh and Ludolph, Ingo and Beier, Justus and Boos, Anja}, doi = {10.1186/s12957-018-1517-0}, faupublication = {yes}, journal = {World Journal of Surgical Oncology}, note = {EVALuna2:211467}, peerreviewed = {Yes}, title = {{Reconstruction} of composite defects of the scalp and neurocranium-a treatment algorithm from local flaps to combined {AV} loop free flap reconstruction}, volume = {16}, year = {2018} } @article{faucris.266497886, abstract = {In addition to the progressive development of surgical oncological techniques for malignant tumors of the rectum, anal canal and vulva, reconstructive procedures after oncological interventions in the perianal region represent a cornerstone in the postoperative quality of life of patients. Modern treatment modalities for rectal cancer with neoadjuvant chemoradiotherapy increase the survival rate and simultaneously reduce the risk of local recurrence to 5-10%, especially by cylindrical extralevatory extirpation of the rectum. The price for increased surgical radicality and improved oncological safety is the acceptance of larger tissue defects. Simple suture closure of perineal wounds often does not primarily heal, resulting in wound dehiscence, surgical site infections and formation of chronic fistulas and sinuses. The interdisciplinary one-stage or two-stage reconstruction of the perianal region with well-vascularized tissue has proven to be a reliable procedure to prevent or control such complications.}, author = {Horch, Raymund E. and Ludolph, Ingo and Arkudas, Andreas}, doi = {10.1007/s00053-021-00575-9}, faupublication = {yes}, journal = {Coloproctology}, note = {CRIS-Team WoS Importer:2021-11-26}, peerreviewed = {No}, title = {{Reconstruction} of oncological defects of the perianal region}, year = {2021} } @article{faucris.258795835, abstract = {In addition to the progressive development of surgical oncological techniques for malignant tumors of the rectum, anal canal and vulva, reconstructive procedures after oncological interventions in the perianal region represent a cornerstone in the postoperative quality of life of patients. Modern treatment modalities for rectal cancer with neoadjuvant chemoradiotherapy increase the survival rate and simultaneously reduce the risk of local recurrence to 5–10%, especially by cylindrical extralevatory extirpation of the rectum. The price for increased surgical radicality and improved oncological safety is the acceptance of larger tissue defects. Simple suture closure of perineal wounds often does not primarily heal, resulting in wound dehiscence, surgical site infections and formation of chronic fistulas and sinuses. The interdisciplinary one-stage or two-stage reconstruction of the perianal region with well-vascularized tissue has proven to be a reliable procedure to prevent or control such complications.}, author = {Horch, Raymund E. and Ludolph, Ingo and Arkudas, Andreas}, doi = {10.1007/s00104-021-01394-w}, faupublication = {yes}, journal = {Chirurg}, keywords = {Abdominoperineal resection; Pelvic floor exenteration; Pelvic floor reconstruction; Perineal hernia; Wound infection}, note = {CRIS-Team Scopus Importer:2021-05-21}, peerreviewed = {Yes}, title = {{Reconstruction} of oncological defects of the perianal region {Rekonstruktion} onkologischer {Defekte} der {Perianalregion}}, year = {2021} } @article{faucris.123151864, abstract = {As microsurgical reconstruction is now being increasingly performed on patients with critical peripheral perfusion and/or arterial occlusive disease in numerous centres, there is a need for standardisation of interdisciplinary diagnostic approach and treatment regimens in such critically ill patients. In a consensus workshop on reconstruction of the vascular compromised lower extremity during the 35th Annual Meeting of the German working group microsurgery of the peripheral nerves and vessels (DAM) in 2013 in Deidesheim, DAM members together with vascular surgeons and interventional radiologists attempted to establish interdisciplinarily routine pathways for diagnosis and therapy and to consolidate key recommendations for treatment.By reviewing the literature and considering the results of the expert meetings, options and limits of therapy were illustrated and recommendations for successful cooperative treatment formulated.By means of interdisciplinary cooperation, limbs can be salvaged and the quality of live as well as survival of patients with severe peripheral vascular disease improved. Different techniques including angioplasty, bypass surgery and microsurgical free flaps can be applied and individualised concepts allow extremity salvage even in patients with severely compromised limbs. Revascularisation provides the possibility of free flap transfer while the risk for the patients is moderate.The poor general condition of the patient requires a sufficient interdisciplinary preoperative planning. By means of interdisciplinary cooperation, the limbs can be salvaged. This not only improves the quality of life but also increases the survival time of patients with occlusive vascular disease. Different concepts for this group of patients have been developed. Surgical treatment with a distal bypass or recanalisation and free flap not only allow for the coverage of large defects, but also represent a haemodynamic advantage by increased blood flow in the bypass. This is attributed to the additional vascular bed that is transplanted with the free flap. Limb salvage means relevant improvement, however, the initially less demanding procedure of amputation must always be considered.}, author = {Daigeler, A. and Kneser, U. and Fansa, H. and Riester, T. and Uder, Michael and Horch, Raymund E.}, doi = {10.1055/s-0034-1385851}, faupublication = {yes}, journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie}, note = {EVALuna2:14357}, pages = {248-55}, peerreviewed = {Yes}, title = {{Reconstruction} of the vascular compromised lower extremity - report of the consensus workshop at the 35. {Meeting} of the {DAM} ({Deutschsprachige} {Gemeinschaft} für {Mikrochirurgie} der peripheren {Nerven} und {Gefäße}) 2013 in {Deidesheim}}, volume = {46}, year = {2014} } @article{faucris.107031584, author = {Horch, Raymund E. and Boccaccini, Aldo R. and Arkudas, Andreas}, doi = {10.4172/2157-7552.1000e121}, faupublication = {yes}, journal = {Journal of Tissue Science & Engineering}, peerreviewed = {Yes}, title = {{Regenerative} {Medicine} and {Tissue} {Engineering}: {How} {Far} {Are} {We}?}, volume = {4}, year = {2013} } @article{faucris.204503773, abstract = {In addition to the impact factor, research funding also plays a central role in evaluating the academic performance and quality of a researcher, a clinic or a surgical specialty. The scope and quality of research in Plastic Surgery are usually very little known, so that even large funding institutions do not get a full view of research funding in our specialty. Therefore, sometimes traditional structures are not adapted to new needs by the developing younger surgical fields. In peer review sometimes peers are not chosen from the same surgical specialty, but from a different surgical fields being peers in large field of surgery. By this a bias can easily be generated which would not be advantageous for subspecialties such Plastic Surgery. The goal of this paper is to establish an overview in the form of a registry of the German Society of Plastic Reconstructive and Aesthetic Surgeons (DGPRÄC) in order to make the joint academic achievements more visible in the future. At the same time, a research funding report is to be published for the years 2015 and 2016. }, author = {Giunta, R. E. and Horch, Raymund E. and Prantl, L. and Fuchs, P. C. and Germann, G. and Infanger, M. and Jakubietz, R. and Kneser, U. and Langer, S. and Lehnhardt, M. and Machens, H. G. and Mailaender, P. and Pallua, N. and Reichert, B. and Schaefer, D. J. and Schaller, H. -E. and Stark, G. B. and Steinau, H. -U. and Vogt, P. M.}, doi = {10.1055/s-0042-121417}, faupublication = {yes}, journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie}, note = {EVALuna2:32824}, pages = {370-373}, peerreviewed = {Yes}, title = {{Registry} {Research} {Funding} of the {German} {Society} of {Plastic}, {Reconstructive} and {Aesthetic} {Surgeons} ({DGPRÄC}) and {Research} {Funding} {Report} 2015/2016}, volume = {48}, year = {2016} } @article{faucris.234229191, abstract = {This report serves to publicize the research of academic institutions for Plastic Surgery within our society DGPRÄC in 2017/2018 and sequels the funding report of 2015/2016. Applications to public, non-public, or industrial funding organizations were evaluated. At the same time, this paper analyses the number of approved DFG applications in Plastic, Thoracic and Vascular Surgery in the GEPRIS system. Contrary to these specialties, Plastic Surgery is not classified as an independent speciality in the subject structure of the DFG review board which results in a lack of transparency concerning Plastic Surgery research work.Our previously established online database (https://docs.google.com/forms/d/1OaSnHyKTysawiI1ie7kfUxDf7nJP{\_}RiTUJTsnb7Mq{\_}E/edit) for reporting requested/ approved and rejected research applications to public, non-public and industrial funding organizations was continued and evaluated together with applications found in the DFG's public database GEPRIS.Compared to the previous year's report, the number of approved applications from public research organizations (DFG, BMBF, BMWi, EU) was increased from 23 to 27. We identified 19 approved DFG applications from Plastic Surgery, as compared to 9 and 8 applications by Thoracic and Vascular Surgery, respectively.Taken together, this data emphasizes that our research is at least equal to that of other newly established surgical specialties within the framework of the DFG. Accordingly, we hope to provide further arguments for an adaptation of the DFG review boards subject structure to include Plastic Surgery as an independent specialty as it is for Vascular Surgery and Thoracic Surgery.}, author = {Giunta, R. E. and Moellhoff, N. and Horch, Raymund E. and Prantl, L. and Beier, J. P. and Daigeler, A. and Dragu, Adrian and Eisenhardt, S. U. and Fuchs, P. C. and Germann, G. and Hirsch, T. and Infanger, M. and Jakubietz, R. and Kneser, U. and Langer, S. and Lehnhardt, M. and Machens, H. G. and Mailander, P. and Reichert, B. and Radtke, Christine and Schaefer, D. J. and Siemers, F. and Stark, G. B. and Steinau, H. U. and Vogt, P. M.}, doi = {10.1055/a-0770-3485}, faupublication = {yes}, journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie}, note = {EVALuna2:50995}, pages = {414-421}, peerreviewed = {Yes}, title = {{Registry} {Research} {Funding} of the {German} {Society} of {Plastic}, {Reconstructive} and {Aesthetic} {Surgeons} ({DGPRÄC}) and {Research} {Funding} {Report} 2017/2018}, volume = {50}, year = {2018} } @article{faucris.243618683, abstract = {Zusammenfassung}, author = {Moellhoff, N. and Prantl, L. and Behr, B. and Beier, J. P. and Daigeler, A. and Dragu, A. and Eisenhardt, S. U. and Fuchs, P. C. and Germann, G. and Hirsch, T. and Horch, Raymund E. and Infanger, M. and Jakubietz, R. and Kneser, U. and Langer, S. and Lehnhardt, M. and Machens, H. G. and Mailaender, P. and Reichert, B. and Radtke, C. and Schaefer, D. J. and Siemers, F. and Stark, G. B. and Steinau, H. U. and Vogt, P. M. and Giunta, R. E.}, doi = {10.1055/a-1267-0388}, faupublication = {yes}, journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie}, note = {CRIS-Team WoS Importer:2020-10-09}, peerreviewed = {Yes}, title = {{Registry} {Research} {Funding} of the {German} {Society} of {Plastic}, {Reconstructive} and {Aesthetic} {Surgeons} ({DGPRaC}) and {Research} {Funding} {Report} 2019/2020}, year = {2020} } @article{faucris.293828610, abstract = {Background Research is an integral part of academic medicine. In plastic surgery, it sets the course for innovations in the specialty. The purpose of this study is to present the research performance of plastic surgeons in Germany for the period 2021/2022 and to compare it with previous periods.Materials and Methods The directors of plastic surgical academic institutions reported all requested/approved and rejected research applications to public, non-public and industrial funding organizations. Data was gathered within an established online database. In addition, the DFG & PRIME;s public database GEPRIS was screened for plastic surgical research grants. Data was also collected regarding research infrastructure and organization at the participating centers.Results 105 applications were reported to 54 different funding agencies from 20 plastic surgery centers. 37 funding applications were submitted to the major public funding agencies DFG, BMBF, BMWi, BMG, BMVg, G-BA and EU. Of these, 59,5% (22/37) were DFG, 13,5% (5/37) each BMBF and EU, 5,4% (2/37) BMWi, and 2,7% (1/37) each BMG, BMVg, and G-BA applications. The average funding volume of these proposals was 401,515 euros. Approved DFG proposals were most frequently assigned to the review board 205-27 Orthopedics, Trauma Surgery, Reconstructive Surgery (n=10/16, 62,5%). Over time, the research registry shows an increase in the number of proposals in general and those granted. 70,0% (14/20) of participating sites had their own experimental research laboratory, while only 40,0% (8/20) had their own clinical trial center.Conclusion The 2021/2022 Research Funding Report once again highlights the impressive research accomplishments of the plastic surgery community.}, author = {Moellhoff, N. and Prantl, L. and Behr, B. and Beier, J. P. and Daigeler, A. and Dragu, A. and Eisenhardt, S. U. and Fuchs, P. C. and Germann, G. and Hirsch, T. and Horch, Raymund E. and Infanger, M. and Jakubietz, R. and Kneser, U. and Langer, S. and Lehnhardt, M. and Machens, H. G. and Mailaender, P. and Reichert, B. and Radtke, C. and Schaefer, D. J. and Siemers, F. and Vogt, P. M. and Menke, H. and Giunta, R. E.}, doi = {10.1055/a-2007-4475}, faupublication = {yes}, journal = {Handchirurgie, Mikrochirurgie, Plastische Chirurgie}, note = {CRIS-Team WoS Importer:2023-03-24}, peerreviewed = {Yes}, title = {{Registry} {Research} {Funding} of the {German} {Society} of {Plastic}, {Reconstructive} and {Aesthetic} {Surgery} ({DGPRaC}) and {Research} {Funding} {Report} 2021/2022}, year = {2023} } @article{faucris.207347210, author = {Ludolph, I. and Horch, Raymund E. and Beier, Justus}, doi = {10.1016/j.bjps.2016.12.020}, faupublication = {yes}, journal = {Journal of Plastic Reconstructive and Aesthetic Surgery}, note = {EVALuna2:32845}, pages = {550-551}, peerreviewed = {No}, title = {{Reply} to letter regarding submission entitled: {Cracking} the perfusion code? - {Laser}-assisted indocyanine green angiography and combined laser {Doppler} spectrophotometry for intraoperative evaluation of tissue perfusion in autologous breast reconstruction with {DIEP} or ms-{TRAM} flaps}, volume = {70}, year = {2017} } @article{faucris.120883004, author = {Saalabian, Ali A. and Horch, Raymund E. and Bermel, Christina and Arkudas, Andreas and Kneser, Ulrich}, doi = {10.1097/PRS.0000000000000790}, faupublication = {yes}, journal = {Plastic and Reconstructive Surgery}, note = {EVALuna2:15584}, pages = {232e}, peerreviewed = {Yes}, title = {{Reply}: vascularization of the dorsal base of the second metacarpal bone: implications for a reverse second metacarpal dorsal artery flap}, volume = {135}, year = {2015} } @article{faucris.111650924, abstract = {Magnetic resonance imaging with arterial spin labeling (ASL) is a noninvasive approach to measure organ perfusion. The purpose of this study was to evaluate the reproducibility of ASL kidney perfusion measurements with semiautomatic segmentation, which allows separate quantification of cortical and medullary perfusion.The right kidneys of 14 healthy volunteers were examined 6 times on 2 occasions (3 times at each occasion). There was a 10-minute pause between each examination and a 14-day interval between the 2 occasions. Cortical, medullary, and whole kidney parenchymal perfusion was determined with customized semiautomatic segmentation software. Coefficient of variances (CVs) and intraclass correlations (ICCs) were calculated.Mean whole, cortical, and medullary kidney perfusion was 307.26 ± 25.65, 337.10 ± 34.83, and 279.61 ± 26.73 mL/min/100 g, respectively. On session 1, mean perfusion for the whole kidney, cortex, and medulla was 307.08 ± 26.91, 336.79 ± 36.54, and 279.60 ± 27.81 mL/min/100 g, respectively, and on session 2, 307.45 ± 24.65, 337.41 ± 33.48, and 279.61 ± 25.94 mL/min/100 g, respectively (P > 0.05; R = 0.60/0.59/0.54). For whole, cortical, and medullary kidney perfusion, the total ICC/CV were 0.97/3.43 ± 0.86%, 0.97/4.19 ± 1.33%, and 0.96/4.12 ± 1.36%, respectively. Measurements did not differ significantly and showed a very good correlation (P > 0.05; R = 0.75/0.76/0.65).ASL kidney measurements combined with operator-independent semiautomatic segmentation revealed high correlation and low variance of cortical, medullary, and whole kidney perfusion.}, author = {Hammon, Matthias and Janka, Rolf Matthias and Siegl, Christian and Seuß, Hannes and Grosso, Roberto and Martirosian, Petros and Schmieder, Roland and Uder, Michael and Kistner, Iris}, doi = {10.1097/MD.0000000000003083}, faupublication = {yes}, journal = {Medicine}, note = {EVALuna2:3854}, pages = {e3083}, peerreviewed = {Yes}, title = {{Reproducibility} of {Kidney} {Perfusion} {Measurements} {With} {Arterial} {Spin} {Labeling} at 1.5 {Tesla} {MRI} {Combined} {With} {Semiautomatic} {Segmentation} for {Differential} {Cortical} and {Medullary} {Assessment}}, volume = {95}, year = {2016} } @inproceedings{faucris.113912744, author = {Daenicke, Jonas and Schubert, Dirk W. and Horch, Raymund E. and Walter, Bastian}, booktitle = {Polychar 22nd}, date = {2014-04-07/2014-04-11}, faupublication = {yes}, peerreviewed = {unknown}, title = {{Resilience} of silicone breast implants – new insights by mapping the mechanical properties of implant}, venue = {Stellenbosch}, year = {2014} } @inproceedings{faucris.123002704, author = {Schubert, Dirk W. and Horch, Raymund E. and Daenicke, Jonas and Walter, Bastian}, booktitle = {14th BIO NANO-MATERIALS}, faupublication = {yes}, peerreviewed = {unknown}, title = {{Resilience} of {Silicone} {Breast} {Implants} – {New} {Insights} by {Mapping} the {Mechanical} {Properties} of {Implant} {Shells}}, venue = {Erlangen}, year = {2013} } @article{faucris.233197404, abstract = {15 micro-textured silicone breast explants are investigated in cyclic compression tests regarding their mechanical behavior. Initially a maximum load of 2.5 kN was applied to the explants in a repetitive way until failure happened. Finally the results are shown on the one hand as a correlation between the number of endured loading cycles and the implantation time. On the other hand the correlation to the explants’ mass was analyzed and described by using a power la}, author = {von Hanstein, Harald and Horch, Raymund E. and Schubert, Dirk W.}, doi = {10.1016/j.polymertesting.2020.106377}, faupublication = {yes}, journal = {Polymer Testing}, keywords = {Silicone breast explant; cyclic load; implantation time; explant mass; compresssion test; mechanical behavior}, peerreviewed = {Yes}, title = {{Resistance} of silicone breast explants under cyclic compressive load as a function of implantation time and explant mass}, volume = {84}, year = {2020} } @article{faucris.264066988, author = {Müller-Seubert, Wibke and Roth, Sascha and Hauck, Theresa and Arkudas, Andreas and Horch, Raymund E. and Ludolph, Ingo}, doi = {10.1111/iwj.13683}, faupublication = {yes}, journal = {International Wound Journal}, note = {CRIS-Team Scopus Importer:2021-09-17}, peerreviewed = {Yes}, title = {{Response} to the letter to the editor ‘{Novel} imaging methods reveal positive impact of topical negative pressure application on tissue perfusion in an in-vivo skin model’}, year = {2021} } @article{faucris.111190904, abstract = {Combined vascular reconstruction and free flap transfer has been established in centers as a feasible therapeutic option in cases with critical limb ischemia (CLI) and large tissue defects otherwise destined for major amputation. However, the number of patients treated with this combined approach is limited, and data regarding long-term follow-up and functional outcome are scarce. We therefore report our 10-year experience in free flap transplantation after vascular reconstruction as a last attempt for limb salvage, with special emphasis of complication rate, limb salvage, and postoperative mobility.CLI patients undergoing combined vascular reconstruction and consequent free flap transfer from 2003 to 2013 were retrospectively observed. Of 80 cases in total, patients with traumatic and oncologic indications were excluded; 33 (mean age, 66 years; range, 51-82 years) of these cases were performed for limb salvage and were included in this study. Long-term follow-up was possible in 32 of 33 patients (mean, 58 months; range, 2-126 months).Thirty-three patients were analyzed. We performed arterial revascularization with 9 arteriovenous loops, 23 bypass grafts (10 popliteal-pedal, 9 femoral-crural, and 4 femoral-popliteal), and 1 venous interposition graft. For defect coverage, tissue transfer was comprised of six different flap entities (10 latissimus dorsi, 2 gracilis, 1 anterior lateral thigh, 7 rectus abdominis, 11 radialis, and 2 greater omentum flaps). Complications occurred in 16 of 33 patients (49%). Early complications included eight acute occlusions of arterial reconstructions; major bleedings were seen in eight patients as well. There were two flap losses and one major amputation in the early postoperative period. No in-hospital deaths were observed. Late results revealed a limb salvage rate of 87% after 1 year and 83% after 5 years. Amputation-free survival was 87% after 1 year and 75% after 5 years. Overall survival was 100% and 87% after 1 year and 5 years, respectively. Follow-up showed 42% of patients with no limitations in ambulation, 54% with maintained preoperative ambulatory status, and one bedridden patient.The combined approach for limb salvage in CLI patients is associated with excellent results in limb salvage and functional outcome in patients who would otherwise be candidates for major amputation, despite an initially elevated complication rate. The option of combined revascularization with free tissue transfer should be evaluated in all mobile patients with CLI, large tissue defects, and exposed tendon or bone structures before major amputation. However, further studies are required to support these results.}, author = {Meyer, Alexander and Goller, Katja and Horch, Raymund E. and Beier, Justus and Taeger, Christian and Arkudas, Andreas and Lang, Werner}, doi = {10.1016/j.jvs.2014.12.005}, faupublication = {yes}, journal = {Journal of Vascular Surgery}, note = {EVALuna2:15203}, pages = {1239-48}, peerreviewed = {Yes}, title = {{Results} of combined vascular reconstruction and free flap transfer for limb salvage in patients with critical limb ischemia}, volume = {61}, year = {2015} } @article{faucris.110735944, abstract = {Free flap transplantation to vascular reconstructions as arteriovenous (AV) loops has been established in centers as a feasible therapeutic option for defect reconstruction in the absence of proper recipient vessels, caused by oncologic resections, radiation, or trauma. We report our 10-year experience in free flap transplantation after vascular reconstruction with special emphasis on complication rate and postoperative mobility.Forty-seven patients (mean age: 60 years, range: 19-86) were included. Defect etiology was posttraumatic in 19 patients; 14 defects were due to oncological resections and seven sternal osteomyelitis; three patients presented with radiation ulcers, two with aseptic femoral head necrosis, and one with defects caused by acne inversa and hip joint prosthesis infection. Long-term follow-up was 45 months (range: 0-126). We performed arterial revascularization with 36 AV loops, eight bypass grafts, and three venous interposition grafts. Subsequent tissue transfer comprised 24 latissimus dorsi, two vastus lateralis, one gracilis, one anterior lateral thigh (ALT), 16 rectus abdominis, one radialis forearm, and two osteocutaneous vascularized fibula flaps.Complications occurred in 25/47 patients (53%). Early complications included five acute occlusions of arterial reconstructions and six major bleedings. There were six flap losses and three major amputations. Two in-hospital deaths were observed (4%). Overall survival accounted for 89.0 and 74.7% after 1 and 5 years, respectively.The 5-year survival rate in long-term follow-up is favorable, despite an initially elevated complication rate. Successful defect coverage can be achieved by this method in a high percentage of patients.}, author = {Meyer, Alexander and Horch, Raymund E. and Schöngart, Elisabeth and Beier, Justus and Taeger, Christian and Arkudas, Andreas and Lang, Werner}, doi = {10.1016/j.bjps.2015.11.025}, faupublication = {yes}, journal = {Journal of Plastic Reconstructive and Aesthetic Surgery}, note = {EVALuna2:15223}, pages = {545-53}, peerreviewed = {Yes}, title = {{Results} of combined vascular reconstruction by means of {AV} loops and free flap transfer in patients with soft tissue defects}, volume = {69}, year = {2016} } @article{faucris.245145459, abstract = {Introduction: Soft tissue reconstruction of the hand and distal upper extremity is challenging to preserve the function of the hand as good as possible. Therefore, a thin flap has been shown to be useful. In this retrospective study, we aimed to show the use of the free temporoparietal fascial flap in soft tissue reconstruction of the hand and distal upper extremity. Methods: We analysed the outcome of free temporoparietal fascial flaps that were used between the years 2007and 2016 at our institution. Major and minor complications, defect location and donor site morbidity were the main fields of interest. Results: 14 patients received a free temporoparietal fascial flap for soft tissue reconstruction of the distal upper extremity. Minor complications were noted in three patients and major complications in two patients. Total flap necrosis occurred in one patient. Conclusion: The free temporoparietal fascial flap is a useful tool in reconstructive surgery of the hand and the distal upper extremity with a low donor site morbidity and moderate rates of major and minor complications.}, author = {Müller-Seubert, Wibke and Horch, Raymund E. and Schmidt, Vanessa and Ludolph, Ingo and Schmitz, Marweh and Arkudas, Andreas}, doi = {10.1007/s00402-020-03635-9}, faupublication = {yes}, journal = {Archives of Orthopaedic and Trauma Surgery}, keywords = {Hand; Reconstruction; Temporoparietal fascial flap; Upper extremity}, note = {CRIS-Team Scopus Importer:2020-11-13}, peerreviewed = {Yes}, title = {{Retrospective} analysis of free temporoparietal fascial flap for defect reconstruction of the hand and the distal upper extremity}, year = {2020} } @article{faucris.119957244, abstract = {Defect reconstruction of the trunk can be performed using microsurgical free flap transplantation. In cases of missing or inappropriate recipient vessels, microsurgical defect reconstruction of the trunk can be achieved by combining free flaps with arteriovenous loops. Here we present our 5-year experience of trunk reconstruction using AV loops and free flaps in a retrospective evaluation. We analyzed 32 cases of trunk reconstruction using a combined approach of free flap transplantation and arteriovenous loops between 2011 and 2016 regarding postoperative complications and perioperative course. Twenty-one patients suffered from sternal defects, 4 from presacral defects, 3 patients presented with lateral chest wall defects, 2 patients suffered from lumbosacral defects, 1 patient had a gluteal defect and 1 patient a defect at the clavicle. In all cases, free flap transplantation and arteriovenous loop creation were performed in a two-stage procedure. There were 8 thromboses of the arteriovenous loops with 4 flap failures. Only 1 flap loss was located in the sternal region, whereas one presacral flap and both lumbosacral flaps were lost. Reconstruction of large soft tissue defects of the trunk by a combined approach with an arteriovenous loop creation and consecutive free flap transplantation represents a reliable procedure in ventral and posterior cranial localization, whereas in the caudal posterior region, AV loop thrombosis can occur. Therefore, to minimize flap loss, a two-stage procedure should be performed.}, author = {Arkudas, Andreas and Horch, Raymund E. and Regus, Susanne and Meyer, Alexander and Lang, Werner and Schmitz, Marweh and Boos, Anja and Ludolph, Ingo and Beier, Justus}, doi = {10.1016/j.bjps.2017.08.025}, faupublication = {yes}, journal = {Journal of Plastic Reconstructive and Aesthetic Surgery}, note = {EVALuna2:15321}, peerreviewed = {Yes}, title = {{Retrospective} cohort study of combined approach for trunk reconstruction using arteriovenous loops and free flaps}, year = {2017} } @article{faucris.269716384, abstract = {Cancer models mimicking the tumor microenvironment are necessary to successfully develop and predict responses of oncological drugs. In this study, electrospun polycaprolactone (PCL) matrices are tested for the development of an in vitro breast cancer model. The effects of fiber thickness and plasma-treatment of the matrices on in vitro growth of breast cancer-associated cells, namely breast cancer cells MDA-MB-231, primary adipose-derived stem cells (ADSCs) and primary endothelial progenitor cells (EPCs) are evaluated. Surface treatment of the matrices by air-plasma leads to increased oxygen/carbon ratio and hydrophilicity. WST-8 analysis reveals that the proliferation of all three cell types increased exponentially over 12 d on all matrices. MDA-MB-231 and ADSC show a higher proliferation on nanofibers due to enhanced cellular adhesion compared to microfibers. In contrast, EPCs show a significantly higher proliferation on microfibers than on nanofibers at day 12 which can further be significantly improved by air-plasma treatment. Cross-sectioning analysis shows that cells grow on the surface of nanofibers, while microfibers have considerable cellular infiltration. These findings suggest that the electrospun PCL matrices are a suitable tool for the development of breast cancer models containing several cell types, which can ultimately lead to designing novel tumor therapies.