% Encoding: UTF-8 @COMMENT{BibTeX export based on data in FAU CRIS: https://cris.fau.de/} @COMMENT{For any questions please write to cris-support@fau.de} @article{faucris.249626924, abstract = {Biofabrication can be a tool to three-dimensionally (3D) print muscle cells embedded inside hydrogel biomaterials, ultimately aiming to mimic the complexity of the native muscle tissue and to createin-vitromuscle analogues for advanced repair therapies and drug testing. However, to 3D print muscle analogues of high cell alignment and synchronous contraction, the effect of biofabrication process parameters on myoblast growth has to be understood. A suitable biomaterial matrix is required to provide 3D printability as well as matrix degradation to create space for cell proliferation, matrix remodelling capacity, and cell differentiation. We demonstrate that by the proper selection of nozzle size and extrusion pressure, the shear stress during extrusion-bioprinting of mouse myoblast cells (C2C12) can achieve cell orientation when using oxidized alginate-gelatin (ADA-GEL) hydrogel bionk. The cells grow in the direction of printing, migrate to the hydrogel surface over time, and differentiate into ordered myotube segments in areas of high cell density. Together, our results show that ADA-GEL hydrogel can be a simple and cost-efficient biodegradable bioink that allows the successful 3D bioprinting and cultivation of C2C12 cellsin-vitroto study muscle engineering.}, author = {Distler, Thomas and Solisito, Aditya and Schneidereit, Dominik and Friedrich, Oliver and Detsch, Rainer and Boccaccini, Aldo R.}, doi = {10.1088/1758-5090/ab98e4}, faupublication = {yes}, journal = {Biofabrication}, keywords = {myoblasts;muscle tissue engineering;3D printing;oxidized alginate;gelatin;hydrogel}, peerreviewed = {Yes}, title = {{3D} printed oxidized alginate-gelatin bioink provides guidance for {C2C12} muscle precursor cell orientation and differentiation via shear stress during bioprinting}, volume = {12}, year = {2020} } @article{faucris.270582348, abstract = {Two-dimensional (2D) cancer models have been the standard for drug development over the past few years, but they frequently do not resemble in vivo properties adequately. 3D models are superior in many aspects and are, therefore, more similar to human pathophysiology. Over the past years, the emerging field of biofabrication has made significant advances, resulting in even more sophisticated 3D models. With this study, a hydrogel is created for biofabrication that is suitable for mimicking the tumor microenvironment in vitro and is further tested as a new vascularized melanoma model in vivo. The alginate/hyaluronic acid/gelatin bioink shows good shape-fidelity, high cell survival rates, and enables successful cultivation of melanoma cells and adipose-derived stem cells as well as cell differentiation in vitro. In vivo, in the arteriovenous loop model, it proves to be a unique method to study melanoma progression, tumor vascularization, and ultimately and reliably metastases in an isolated and controlled environment. These results show that this 3D model is very application-oriented for molecular research and therapy developmen}, author = {Schmid, Rafael and Schmidt, Sonja and Detsch, Rainer and Horder, Hannes and Blunk, Torsten and Schrüfer, Stefan and Schubert, Dirk W. and Fischer, Lena and Thievessen, Ingo and Heltmann-Meyer, Stefanie and Steiner, Dominik and Schneidereit, Dominik and Friedrich, Oliver and Grüneboom, Anika and Amouei, Hanna and Wajant, Harald and Horch, Raymund E. and Boßerhoff, Anja Katrin and Arkudas, Andreas and Kengelbach-Weigand, Annika}, doi = {10.1002/adfm.202107993}, faupublication = {yes}, journal = {Advanced Functional Materials}, peerreviewed = {Yes}, title = {{A} new printable alginate / hyaluronic acid / gelatin hydrogel suitable for biofabrication of in vitro and in vivo metastatic melanoma models}, url = {https://onlinelibrary.wiley.com/doi/full/10.1002/adfm.202107993}, year = {2021} } @article{faucris.270640008, author = {Schmid, Rafael and Schmidt, Sonja and Detsch, Rainer and Horder, Hannes and Blunk, Torsten and Schrüfer, Stefan and Schubert, Dirk W. and Fischer, Lena and Thievessen, Ingo and Heltmann-Meyer, Stefanie and Steiner, Dominik and Schneidereit, Dominik and Friedrich, Oliver and Grüneboom, Anika and Amouei, Hanna and Wajant, Harald and Horch, Raymund E. and Boßerhoff, Anja Katrin and Arkudas, Andreas and Kengelbach-Weigand, Annika}, doi = {10.1002/adfm.202107993}, faupublication = {yes}, journal = {Advanced Functional Materials}, month = {Jan}, peerreviewed = {Yes}, title = {{A} new printable alginate / hyaluronic acid / gelatin hydrogel suitable for biofabrication of in vitro and in vivo metastatic melanoma models}, volume = {Volume 32}, year = {2022} } @article{faucris.258190286, abstract = {Biofabrication, including printing technologies, has emerged as a powerful approach to the design of disease models, such as in cancer research. In breast cancer, adipose tissue has been acknowledged as an important part of the tumor microenvironment favoring tumor progression. Therefore, in this study, a 3D-printed breast cancer model for facilitating investigations into cancer cell-adipocyte interaction was developed. First, we focused on the printability of human adipose-derived stromal cell (ASC) spheroids in an extrusion-based bioprinting setup and the adipogenic differentiation within printed spheroids into adipose microtissues. The printing process was optimized in terms of spheroid viability and homogeneous spheroid distribution in a hyaluronic acid-based bioink. Adipogenic differentiation after printing was demonstrated by lipid accumulation, expression of adipogenic marker genes, and an adipogenic ECM profile. Subsequently, a breast cancer cell (MDA-MB-231) compartment was printed onto the adipose tissue constructs. After nine days of co-culture, we observed a cancer cell-induced reduction of the lipid content and a remodeling of the ECM within the adipose tissues, with increased fibronectin, collagen I and collagen VI expression. Together, our data demonstrate that 3D-printed breast cancer-adipose tissue models can recapitulate important aspects of the complex cell-cell and cell-matrix interplay within the tumor-stroma microenvironment.}, author = {Horder, Hannes and Lasheras, Mar Guaza and Grummel, Nadine and Nadernezhad, Ali and Herbig, Johannes and Erguen, Sueleyman and Tessmar, Joerg and Groll, Juergen and Fabry, Ben and Bauer-Kreisel, Petra and Blunk, Torsten}, doi = {10.3390/cells10040803}, faupublication = {yes}, journal = {Cells}, note = {CRIS-Team WoS Importer:2021-05-14}, peerreviewed = {Yes}, title = {{Bioprinting} and {Differentiation} of {Adipose}-{Derived} {Stromal} {Cell} {Spheroids} for a {3D} {Breast} {Cancer}-{Adipose} {Tissue} {Model}}, volume = {10}, year = {2021} } @article{faucris.276685712, abstract = {The development of hydrogels suitable for biofabrication is essential to enable advanced approaches for tissue engineering and regenerative medicine. Applications in both hard and soft tissues require tailor-made bioinks to guide cellular behavior in a 3D matrix. In this study we aimed to enhance the stability and adjust the degradation behavior of alginate dialdehyde-gelatine (ADA-GEL) hydrogels using an in-situ crosslinking mechanism additionally to external crosslinking. To test this approach, we added 0.1 and 0.5% (w/v) bioactive inorganic fillers (BIF) based on sub-micrometric calcium-silicate particles to ADA-GEL hydrogels. Such BIF release bivalent Ca2+ ions which can internally crosslink alginate chains and tune the degradation of the hydrogel over 28 days of incubation. It was found that pure ADA-GEL dissolved quickly after the first day while the composite hydrogels remained stable exhibiting reduced degradation (20–50% of the initial weight). 3D (bio)printing of composite bioinks revealed improved printing accuracy, 3D shape fidelity as well as cell spreading throughout the entire matrix during 14 days of evaluation. Chemically, BIF did not seem to have an influence on ADA-GEL Schiff's base bonds and also the mechanical properties of the composite hydrogels were comparable to those of pure ADA-GEL with Young's moduli of about 4 kPa. Comprehensive rheology measurements were carried out to determine printing parameters and the influence of BIF on the bioprinting process. We conclude that the novel hydrogel composition based on ADA-GEL system incorporating calcium-silicate BIF exhibits tunable behavior in vitro up to at least 28 days of incubation leading to improved stability and time-dependent cell behavior in 3D bioprinted constructs.}, author = {Heid, Susanne and Becker, Kevin and Byun, Jiwon and Biermann, Isabell and Neščáková, Zuzana and Zhu, Hui and Groll, Jürgen and Boccaccini, Aldo R.}, doi = {10.1016/j.bprint.2022.e00200}, faupublication = {yes}, journal = {Bioprinting}, keywords = {Alginate dialdehyde; Bioactive glass; Bioprinting; Composite bioink; Fibroblasts; Gelatin; Soft tissue engineering}, note = {CRIS-Team Scopus Importer:2022-06-10}, peerreviewed = {Yes}, title = {{Bioprinting} with bioactive alginate dialdehyde-gelatin ({ADA}-{GEL}) composite bioinks: {Time}-dependent in-situ crosslinking via addition of calcium-silicate particles tunes in vitro stability of {3D} bioprinted constructs}, volume = {26}, year = {2022} } @article{faucris.216187886, abstract = {Cell migration through the extracellular matrix is governed by the interplay between cell-generated propulsion forces, adhesion forces, and resisting forces arising from the steric hindrance of the matrix. Steric hindrance in turn depends on matrix porosity, matrix deformability, cell size, and cell deformability. In this study, we investigate how cells respond to changes in steric hindrance that arise from altered cell mechanical properties. Specifically, we measure traction forces, cell morphology, and invasiveness of MDA-MB 231 breast cancer cells in three-dimensional collagen gels. To modulate cell mechanical properties, we either decrease nuclear deformability by twofold overexpression of the nuclear protein lamin A or we introduce into the cells stiff polystyrene beads with a diameter larger than the average matrix pore size. Despite this increase of steric hindrance, we find that cell invasion is only marginally inhibited, as measured by the fraction of motile cells and the mean invasion depth. To compensate for increased steric hindrance, cells employ two alternative strategies. Cells with higher nuclear stiffness increase their force polarity, whereas cells with large beads increase their net contractility. Under both conditions, the collagen matrix surrounding the cells stiffens dramatically and carries increased strain energy, suggesting that increased force polarity and increased net contractility are functionally equivalent strategies for overcoming an increased steric hindrance.}, author = {Condor, Mar and Mark, Christoph and Gerum, Richard and Grummel, Nadine and Bauer, Andreas and Garcia-Aznar, Jose M. and Fabry, Ben}, doi = {10.1016/j.bpj.2019.02.029}, faupublication = {yes}, journal = {Biophysical Journal}, note = {CRIS-Team WoS Importer:2019-04-18}, pages = {1305-1312}, peerreviewed = {Yes}, title = {{Breast} {Cancer} {Cells} {Adapt} {Contractile} {Forces} to {Overcome} {Steric} {Hindrance}}, volume = {116}, year = {2019} } @inproceedings{faucris.235774922, address = {CAMBRIDGE}, author = {Böhringer, David and Mark, Christoph and Grummel, Nadine and Strissel, Pamela and Strick, Reiner and Grundy, Thomas J. and O'Neill, Geraldine M. and Fabry, Ben}, booktitle = {BIOPHYSICAL JOURNAL}, date = {2020-02-15/2020-02-19}, doi = {10.1016/j.bpj.2019.11.3275}, faupublication = {yes}, note = {CRIS-Team WoS Importer:2020-03-13}, pages = {606A-606A}, peerreviewed = {unknown}, publisher = {CELL PRESS}, title = {{Collective} {Synchronization} of {Contractile} {Forces} in {Tumor} {Spheroids}}, venue = {San Diego, CA}, year = {2020} } @article{faucris.241985972, abstract = {Bioprinting offers the opportunity to fabricate precise 3D tumor models to study tumor pathophysiology and progression. However, the choice of the bioink used is important. In this study, cell behavior was studied in three mechanically and biologically different hydrogels (alginate, alginate dialdehyde crosslinked with gelatin (ADA–GEL), and thiol-modified hyaluronan (HA-SH crosslinked with PEGDA)) with cells from breast cancer (MDA-MB-231 and MCF-7) and melanoma (Mel Im and MV3), by analyzing survival, growth, and the amount of metabolically active, living cells via WST-8 labeling. Material characteristics were analyzed by dynamic mechanical analysis. Cell lines revealed significantly increased cell numbers in low-percentage alginate and HA-SH from day 1 to 14, while only Mel Im also revealed an increase in ADA–GEL. MCF-7 showed a preference for 1% alginate. Melanoma cells tended to proliferate better in ADA– GEL and HA-SH than mammary carcinoma cells. In 1% alginate, breast cancer cells showed equally good proliferation compared to melanoma cell lines. A smaller area was colonized in high-percentage alginate-based hydrogels. Moreover, 3% alginate was the stiffest material, and 2.5% ADA–GEL was the softest material. The other hydrogels were in the same range in between. Therefore, cellular responses were not only stiffness-dependent. With 1% alginate and HA-SH, we identified matrices that enable proliferation of all tested tumor cell lines while maintaining expected tumor heterogeneity. By adapting hydrogels, differences could be accentuated. This opens up the possibility of understanding and analyzing tumor heterogeneity by biofabrication.}, author = {Schmid, Rafael and Schmidt, Sonja and Hazur, Jonas and Detsch, Rainer and Maurer, Evelyn and Boccaccini, Aldo R. and Hauptstein, Julia and Teßmar, Jörg and Blunk, Torsten and Schrüfer, Stefan and Schubert, Dirk W. and Horch, Raymund E. and Boßerhoff, Anja Katrin and Arkudas, Andreas and Kengelbach-Weigand, Annika}, doi = {10.3390/cancers12082320}, faupublication = {yes}, journal = {Cancers}, keywords = {Biofabrication; Breast cancer; Hydrogel; Melanoma; Tumor heterogeneity}, note = {CRIS-Team Scopus Importer:2020-08-28}, pages = {1-21}, peerreviewed = {Yes}, title = {{Comparison} of hydrogels for the development of well-defined 3d cancer models of breast cancer and melanoma}, volume = {12}, year = {2020} } @article{faucris.249628620, abstract = {Biomimetically designed medical-grade polycaprolactone (mPCL) dressings are 3D-printed with pore architecture and anisotropic mechanical characteristics that favor skin wound healing with reduced scarring. Melt electrowritten mPCL dressings are seeded with human gingival tissue multipotent mesenchymal stem/stromal cells and cryopreserved using a clinically approved method. The regenerative potential of fresh or frozen cell-seeded mPCL dressing is compared in a splinted full-thickness excisional wound in a rat model over six weeks. The application of 3D-printed mPCL dressings decreased wound contracture and significantly improved skin regeneration through granulation and re-epithelialization compared to control groups. Combining 3D-printed biomimetic wound dressings and precursor cell delivery enhances physiological wound closure with reduced scar tissue formation.}, author = {Shafiee, Abbas and Cavalcanti, Amanda and Saidy, Navid T and Schneidereit, Dominik and Friedrich, Oliver and Ravichandran, Akhilandeshwari and De-Juan-Pardo, Elena M and Hutmacher, Dietmar W}, doi = {10.1016/j.biomaterials.2020.120558}, faupublication = {yes}, journal = {Biomaterials}, month = {Jan}, pages = {120558}, peerreviewed = {Yes}, title = {{Convergence} of {3D} printed biomimetic wound dressings and adult stem cell therapy.}, volume = {268}, year = {2021} } @article{faucris.244294049, abstract = {Natural killer (NK) cells are important effector cells in the immune response to cancer. Clinical trials on adoptively transferred NK cells in patients with solid tumors, however, have thus far been unsuccessful. As NK cells need to pass stringent safety evaluation tests before clinical use, the cells are cryopreserved to bridge the necessary evaluation time. Standard degranulation and chromium release cytotoxicity assays confirm the ability of cryopreserved NK cells to kill target cells. Here, we report that tumor cells embedded in a 3-dimensional collagen gel, however, are killed by cryopreserved NK cells at a 5.6-fold lower rate compared to fresh NK cells. This difference is mainly caused by a 6-fold decrease in the fraction of motile NK cells after cryopreservation. These findings may explain the persistent failure of NK cell therapy in patients with solid tumors and highlight the crucial role of a 3-D environment for testing NK cell function.}, author = {Mark, Christoph and Czerwinski, Tina and Roessner, Susanne and Mainka, Astrid and Hörsch, Franziska and Heublein, Lucas and Winterl, Alexander and Sanokowski, Sebastian and Richter, Sebastian and Bauer, Nina and Angelini, Thomas E. and Schuler, Gerold and Fabry, Ben and Bosch-Voskens, Caroline}, doi = {10.1038/s41467-020-19094-0}, faupublication = {yes}, journal = {Nature Communications}, note = {CRIS-Team Scopus Importer:2020-10-23}, peerreviewed = {Yes}, title = {{Cryopreservation} impairs 3-{D} migration and cytotoxicity of natural killer cells}, volume = {11}, year = {2020} } @inproceedings{faucris.267073411, address = {OXFORD}, author = {Hesselbarth, Ramona and Esser, Tilman and Roshanbinfar, Kaveh and Schrüfer, Stefan and Schubert, Dirk W. and Engel, Felix}, booktitle = {EUROPEAN HEART JOURNAL}, doi = {10.1093/eurheartj/ehab724.3234}, faupublication = {yes}, note = {CRIS-Team WoS Importer:2021-12-10}, pages = {3234-3234}, peerreviewed = {unknown}, publisher = {OXFORD UNIV PRESS}, title = {{Enhancement} of engineered cardiac tissues by promotion of {hiPSC}-cardiomyocyte proliferation}, year = {2021} } @article{faucris.311100881, abstract = {Aerophilic surfaces immersed underwater trap films of air known as plastrons. Plastrons have typically been considered impractical for underwater engineering applications due to their metastable performance. Here, we describe aerophilic titanium alloy (Ti) surfaces with extended plastron lifetimes that are conserved for months underwater. Long-term stability is achieved by the formation of highly rough hierarchically structured surfaces via electrochemical anodization combined with a low-surface-energy coating produced by a fluorinated surfactant. Aerophilic Ti surfaces drastically reduce blood adhesion and, when submerged in water, prevent adhesion of bacteria and marine organisms such as barnacles and mussels. Overall, we demonstrate a general strategy to achieve the long-term stability of plastrons on aerophilic surfaces for previously unattainable underwater applications.}, author = {Tesler, Alexander and Kolle, Stefan and Prado, Lucia and Thievessen, Ingo and Böhringer, David and Backholm, Matilda and Karunakaran, Bhuvaneshwari and Nurmi, Heikki A. and Latikka, Mika and Fischer, Lena and Stafslien, Shane and Cenev, Zoran M. and Timonen, Jaakko V.I. and Bruns, Mark and Mazare, Anca Valentina and Lohbauer, Ulrich and Virtanen, Sannakaisa and Fabry, Ben and Schmuki, Patrik and Ras, Robin H.A. and Aizenberg, Joanna and Goldmann, Wolfgang}, doi = {10.1038/s41563-023-01670-6}, faupublication = {yes}, journal = {Nature Materials}, note = {CRIS-Team Scopus Importer:2023-09-29}, peerreviewed = {unknown}, title = {{Long}-term stability of aerophilic metallic surfaces underwater}, year = {2023} } @article{faucris.251853905, abstract = {
3D-printing technologies, such as biofabrication, capitalize on the homogeneous distribution and growth of cells inside biomaterial hydrogels, ultimately aiming to allow for cell differentiation, matrix remodeling, and functional tissue analogues. However, commonly, only the mechanical properties of the bioinks or matrix materials are assessed, while the detailed influence of cells on the resulting mechanical properties of hydro- gels remains insufficiently understood. Here, we investigate the properties of hydrogels containing cells and spherical PAAm microgel beads through multi-modal complex mechanical analyses in the small- and large- strain regimes. We evaluate the individual contributions of different filler concentrations and a non-fibrous oxi- dized alginate-gelatin hydrogel matrix on the overall mechanical behavior in compression, tension, and shear. Through material modeling, we quantify parameters that describe the highly nonlinear mechanical response of soft composite materials. Our results show that the stiffness significantly drops for cell- and bead concen- trations exceeding four million per milliliter hydrogel. In addition, hydrogels with high cell concentrations (≥6 mio ml−1) show more pronounced material nonlinearity for larger strains and faster stress relaxation. Our findings highlight cell concentration as a crucial parameter influencing the final hydrogel mechanics, with implications for microgel bead drug carrier-laden hydrogels, biofabrication, and tissue engineering.