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@article{faucris.264852043,
abstract = {ABSTRACT: The current understanding on the clinical efficacy of Rho-associated protein kinase (ROCK) inhibitor for treating Fuchs endothelial corneal dystrophy is summarized to clarify whether the "off-label" ROCK-inhibitor eye-drop application are appropriate. ROCK-inhibitor eye drops may eventually be deemed a cutting-edge therapy for Fuchs endothelial corneal dystrophy patients with acute corneal endothelial defect.},
author = {Kinoshita, Shigeru and Colby, Kathryn A. and Kruse, Friedrich},
doi = {10.1097/ICO.0000000000002642},
faupublication = {yes},
journal = {Cornea},
note = {CRIS-Team Scopus Importer:2021-10-08},
pages = {1225-1228},
peerreviewed = {Yes},
title = {{A} {Close} {Look} at the {Clinical} {Efficacy} of {Rho}-{Associated} {Protein} {Kinase} {Inhibitor} {Eye} {Drops} for {Fuchs} {Endothelial} {Corneal} {Dystrophy}},
volume = {40},
year = {2021}
}
@article{faucris.119417804,
abstract = {Fuchs endothelial corneal dystrophy (FECD) is a slowly progressive bilateral disease of corneal endothelium in which accumulation of extracellular matrix (ECM) and loss of corneal endothelial cells (CECs) are phenotypic features. The corneal endothelium maintains corneal transparency by regulating water hydration; consequently, corneal endothelial dysfunction causes serious vision loss. The only therapy for corneal haziness due to corneal endothelial diseases, including FECD, is corneal transplantation using donor corneas, and no pharmaceutical treatment is available. We provide evidence that the expression levels of transforming growth factor-? (TGF-?) isoforms and TGF-? receptors are high in the corneal endothelium of patients with FECD. A cell model based on patients with FECD shows that TGF-? signaling induced a chronic overload of ECM proteins to the endoplasmic reticulum (ER), thereby enhancing the formation of unfolded protein and triggering the intrinsic apoptotic pathway through the unfolded protein response (UPR). We propose that inhibition of TGF-? signaling may represent a novel therapeutic target that suppresses cell loss as well as the accumulation of ECM in FECD.},
author = {Okumura, Naoki and Hashimoto, Keisuke and Kitahara, Miu and Okuda, Hirokazu and Ueda, Emi and Watanabe, Kyoko and Nakahara, Makiko and Sato, Takahiko and Kinoshita, Shigeru and Tourtas, Theofilos and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich and Koizumi, Noriko},
doi = {10.1038/s41598-017-06924-3},
faupublication = {yes},
journal = {Scientific Reports},
note = {EVALuna2:21350},
pages = {6801},
peerreviewed = {Yes},
title = {{Activation} of {TGF}-β signaling induces cell death via the unfolded protein response in {Fuchs} endothelial corneal dystrophy},
volume = {7},
year = {2017}
}
@article{faucris.107257964,
author = {Stafiej, Piotr and Küng, Florian and Thieme, Daniel and Czugala, Marta and Kruse, Friedrich and Schubert, Dirk W. and Fuchsluger, Thomas},
doi = {10.1016/j.msec.2016.10.058},
faupublication = {yes},
journal = {Advances in Materials Science and Engineering},
peerreviewed = {Yes},
title = {{Adhesion} and metabolic activity of human corneal cells on {PCL} based nanofiber matrices},
year = {2017}
}
@article{faucris.119554644,
abstract = {In this work, polycaprolactone (PCL) was used as a basic polymer for electrospinning of random and aligned nanofiber matrices. Our aim was to develop a biocompatible substrate for ophthalmological application to improve wound closure in defects of the cornea as replacement for human amniotic membrane. We investigated whether blending the hydrophobic PCL with poly (glycerol sebacate) (PGS) or chitosan (CHI) improves the biocompatibility of the matrices for cell expansion. Human corneal epithelial cells (HCEp) and human corneal keratocytes (HCK) were used for in vitro biocompatibility studies. After optimization of the electrospinning parameters for all blends, scanning electron microscopy (SEM), attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), and water contact angle were used to characterize the different matrices. Fluorescence staining of the F-actin cytoskeleton of the cells was performed to analyze the adherence of the cells to the different matrices. Metabolic activity of the cells was measured by cell counting kit-8 (CCK-8) for 20days to compare the biocompatibility of the materials. Our results show the feasibility of producing uniform nanofiber matrices with and without orientation for the used blends. All materials support adherence and proliferation of human corneal cell lines with oriented growth on aligned matrices. Although hydrophobicity of the materials was lowered by blending PCL, no increase in biocompatibility or proliferation, as was expected, could be measured. All tested matrices supported the expansion of human corneal cells, confirming their potential as substrates for biomedical applications.},
author = {Stafiej, Piotr and Küng, Florian and Thieme, Daniel and Czugala, Marta and Kruse, Friedrich and Schubert, Dirk W. and Fuchsluger, Thomas},
doi = {10.1016/j.msec.2016.10.058},
faupublication = {yes},
journal = {Materials Science and Engineering C},
pages = {764-770},
peerreviewed = {unknown},
title = {{Adhesion} and metabolic activity of human corneal cells on {PCL} based nanofiber matrices.},
volume = {71},
year = {2017}
}
@inproceedings{faucris.242416652,
address = {ROCKVILLE},
author = {Hohberger, Bettina and Woern, Max and Lämmer, Robert and Herrmann, Martin and Mardin, Christian and Kruse, Friedrich and Kunze, Rudolf and Wallukat, Gerd},
booktitle = {INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE},
date = {2020-05-01/2020-05-07},
doi = {10.3389/fimmu.2021.550236},
faupublication = {yes},
note = {CRIS-Team WoS Importer:2020-09-11},
peerreviewed = {unknown},
publisher = {ASSOC RESEARCH VISION OPHTHALMOLOGY INC},
title = {{Agonistic} beta 2-adrenergic receptor autoantibodies characterize the aqueous humor of patients with primary and secondary open-angle glaucoma},
venue = {ONLINE},
year = {2020}
}
@article{faucris.110846604,
abstract = {To perform a fellow eye comparison of outcomes and complications when using air or sulfur hexafluoride (SF6) gas as a tamponade in Descemet membrane endothelial keratoplasty (DMEK).One hundred thirty-six eyes of 68 consecutive patients who underwent uneventful DMEK in both eyes for Fuchs endothelial corneal dystrophy were included in this retrospective study. Inclusion criteria were air tamponade (80% of the anterior chamber volume) in the first eye and 20% SF6 gas tamponade (80% of the anterior chamber volume) in the second eye; and same donor tissue culture condition in both eyes. All eyes received laser iridotomy on the day before DMEK. Main outcome measures included preoperative and postoperative best-corrected visual acuity, endothelial cell density, corneal volume, rebubbling rate, and rate of postoperative pupillary block caused by the air/gas bubble.Thirteen of 68 eyes (19.1%) with an air tamponade needed rebubbling compared with 4 of 68 eyes (5.9%) with an SF6 gas tamponade (P = 0.036). Postoperative pupillary block necessitating partial release of air/gas occurred in 1 eye (1.5%) with an air tamponade and 3 eyes (4.4%) with an SF6 gas tamponade (P = 0.301). There were no significant differences in preoperative and postoperative best-corrected visual acuity, endothelial cell density, and corneal volume within 3-month follow-up.Our results confirm the previously reported better graft adhesion when using an SF6 gas tamponade in DMEK without increased endothelial cell toxicity. The rate of pupillary block in eyes with an SF6 gas tamponade was comparable to that with an air tamponade. As a consequence, we recommend using SF6 gas as the tamponade in DMEK.},
author = {von Marchtaler, Philipp and Weller, Julia M. and Kruse, Friedrich and Tourtas, Theofilos},
doi = {10.1097/ICO.0000000000001413},
faupublication = {yes},
journal = {Cornea},
note = {EVALuna2:21352},
peerreviewed = {Yes},
title = {{Air} {Versus} {Sulfur} {Hexafluoride} {Gas} {Tamponade} in {Descemet} {Membrane} {Endothelial} {Keratoplasty}: {A} {Fellow} {Eye} {Comparison}},
year = {2017}
}
@inproceedings{faucris.208385257,
author = {Berner, Daniel and Zenkel, Matthias and Pasutto, Francesca and Schoedel, Johannes and Reis, André and Kruse, Friedrich and Schlötzer-Schrehardt, Ursula},
faupublication = {yes},
note = {EVALuna2:34538},
peerreviewed = {Yes},
title = {{Alternative} splicing and nonsense-mediated {mRNA} decay contribute to regulation of {LOXL1} expression in response to cellular stress in pseudoexfoliation},
volume = {58},
year = {2017}
}
@article{faucris.119790484,
abstract = {To evaluate the efficacy and safety of combined feeder vessel coagulation and topical antiangiogenic therapy using bevacizumab in the treatment of mature corneal blood vessels.Sixteen eyes of 16 patients with mature corneal neovascularization (NV) due to different underlying corneal diseases underwent fine-needle feeder vessel coagulation by diathermy and were treated postoperatively for up to 4 weeks with topical bevacizumab eye drops (containing 5 mg/mL bevacizumab) 5 times a day. Nine patients received an additional subconjunctival bevacizumab injection at the time of cautery.The mean duration of follow-up was 276 ± 147.3 days (range, 29-464 days). Regression of the feeder vessel was observed in 14 eyes. The vascularized area was reduced significantly (P < 0.05). Combined subconjunctival and eye drop antivascular endothelial growth factor treatment was significantly more effective in reducing the vascularized area compared with antivascular endothelial growth factor eye drop therapy alone (P < 0.05). Five patients (5 eyes) needed a second treatment. Thirteen patients (13 eyes) receiving topical bevacizumab treatment combined with feeder vessel coagulation showed stable visual acuity. Two patients had improved visual acuity. One patient had enlarged area of lipid keratopathy despite successful treatment of corneal NV and thus decreased visual acuity. Overall, there was a nonsignificant improvement of best-corrected visual acuity (P > 0.05).In this pilot study, fine-needle feeder vessel coagulation combined with topical bevacizumab application for treatment of mature corneal NV seemed to be a well-tolerated new treatment option to regress corneal NV. This may not only improve corneal transparency but also "preconditions" such a cornea for future keratoplasty.},
author = {Koenig, Yanyan and Bock, Felix and Kruse, Friedrich and Stock, Klaus-Peter and Cursiefen, Claus},
doi = {10.1097/ICO.0b013e31823f8f7a},
faupublication = {yes},
journal = {Cornea},
note = {EVALuna2:21110},
pages = {887-92},
peerreviewed = {Yes},
title = {{Angioregressive} pretreatment of mature corneal blood vessels before keratoplasty: fine-needle vessel coagulation combined with anti-{VEGFs}},
volume = {31},
year = {2012}
}
@article{faucris.119790924,
abstract = {Collagen cross-linking using UV-A irradiation combined with the photosensitizer riboflavin is a new technique for treating progressive keratoconus. The purposes of this study were to examine whether primary human corneal keratocytes (HCKs) are capable of expressing and secreting fibronectin and tissue transglutaminase (tTgase), an enzyme cross-linking extracellular matrix protein, and to examine whether fibronectin and tTgase are increased after the treatment of HCK cells with UV-A irradiation combined with riboflavin (RFUV-A), thus providing another possible physiological mechanism of the cross-linking pathway.Cell cultures established from HCKs were treated with 0.025% riboflavin solution and UV-A (370 nm) irradiance 3 mW/cm2 for 30 minutes. Induction of fibronectin and tTgase was investigated by immunohistochemistry, real-time polymerase chain reaction, and Western blot analysis. Cell viability was quantified by a microscopic live-dead assay. External tTgase activity was measured by the ability to form polymerized fibronectin and the incorporation of biotinylated cadaverine into fibronectin.Treatment of cultured HCK cells with RFUV-A increased the fibronectin and tTgase messenger RNA and protein levels. This effect was not observed in cells treated with riboflavin or UV-A radiation alone. Incorporation of biotinylated cadaverine was significantly increased when HCK cells were treated with RFUV-A.The enzymes tTgase and fibronectin are expressed by RFUV-A treatment in cultured HCK cells. This mechanism provides more information about the physiology of corneal cross-linking.},
author = {Kopsachilis, Nikolaos and Tsaousis, Konstantinos T. and Tsinopoulos, Ioannis T. and Kruse, Friedrich and Welge-Luessen, Ulrich},
doi = {10.1097/ICO.0b013e31828a760d},
faupublication = {yes},
journal = {Cornea},
note = {EVALuna2:21144},
pages = {1034-9},
peerreviewed = {Yes},
title = {{A} novel mechanism of {UV}-{A} and riboflavin-mediated corneal cross-linking through induction of tissue transglutaminases},
volume = {32},
year = {2013}
}
@article{faucris.123096424,
abstract = {Sutures are a common way to attach scaffolds in patients. For tubular cardiac scaffolds, the 'suture retention strength' is commonly used to evaluate the resistance of a scaffold against the pull-out of a suture. In order to make this quantity accessible for ophthalmological scaffolds the test procedure has been modified in a novel way. Polycaprolactone (PCL) films of different thicknesses and an amniotic membrane (AM) were used for the experiments. Circular samples with a radius of 7mm were taken and a suture was passed through each sample and tied to a loop. The sample was clamped in a tensile tester and a bolt was passed through the loop. The suture was then pulled with a constant deformation rate until pull-out occurred. The suture retention strength, the deformation at the suture retention strength, and the deformation at rupture were determined for each sample. The presented modified suture retention test allows to measure the relevant parameters of samples on the scale of ophthalmological scaffolds in a reproducible way. A comparison between the first data on PCL and AM has been made.},
author = {Küng, Florian and Schubert, Dirk W. and Stafiej, Piotr and Kruse, Friedrich and Fuchsluger, Thomas},
doi = {10.1016/j.msec.2016.07.052},
faupublication = {yes},
journal = {Materials Science and Engineering C},
pages = {941-6},
peerreviewed = {unknown},
title = {{A} novel suture retention test for scaffold strength characterization in ophthalmology.},
volume = {69},
year = {2016}
}
@article{faucris.120082644,
abstract = {To analyze the influence of the size of the air bubble subsequent to Descemet membrane endothelial keratoplasty (DMEK) surgery on the rate of graft detachment and need for rebubbling, the incidence of pupillary block, and the observed endothelial cell loss.This is a single-center, retrospective, consecutive case series of 74 cases undergoing DMEK and fulfilling the inclusion criteria concerning the size of the air bubble at the end of surgery. Based on the medical records, patients were divided into 2 groups (n = 37, respectively). The first group had an air bubble with a volume of approximately 50% and the second group of approximately 80% of the anterior chamber (AC) volume, respectively. Patients who did not comply with instructions to remain in the supine position until complete resorption of AC air or cases in which difficulties in graft preparation (eg, radial breaks) occurred were excluded from data analysis. The central corneal thickness and endothelial cell density were measured 6 months after surgery.Ten of 37 patients (27.0%) in the 50% air bubble group and 3 of 37 patients (8.1%) in the 80% air bubble group needed 1 rebubbling procedure (P = 0.032). There was no difference between the groups after 6 months regarding endothelial cell density and central corneal thickness. No pupillary block was observed.Larger air bubbles of 80% anterior chamber volume decrease the risk of graft detachment after DMEK with no detrimental effect on the outcome and risk for pupillary block.},
author = {Cirkovic, Aleksandar and Beck, Christina and Weller, Julia M. and Kruse, Friedrich and Tourtas, Theofilos},
doi = {10.1097/ICO.0000000000000753},
faupublication = {yes},
journal = {Cornea},
note = {EVALuna2:21286},
pages = {482-5},
peerreviewed = {Yes},
title = {{Anterior} {Chamber} {Air} {Bubble} to {Achieve} {Graft} {Attachment} {After} {DMEK}: {Is} {Bigger} {Always} {Better}?},
volume = {35},
year = {2016}
}
@inproceedings{faucris.228451051,
address = {ROCKVILLE},
author = {Zenkel, Matthias and Berner, Daniel and Hoja, Ursula and Pasutto, Francesca and Kruse, Friedrich and Schlötzer-Schrehardt, Ursula},
booktitle = {INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE},
date = {2019-04-28/2019-05-02},
faupublication = {yes},
note = {CRIS-Team WoS Importer:2019-10-29},
peerreviewed = {unknown},
publisher = {ASSOC RESEARCH VISION OPHTHALMOLOGY INC},
title = {{A} potential role for impaired retinoic acid signaling in the pathophysiology of pseudoexfoliation syndrome/glaucoma},
venue = {Vancouver, CANADA},
year = {2019}
}
@article{faucris.251039397,
abstract = {Importance: Exfoliation syndrome is a systemic disorder characterized by progressive accumulation of abnormal fibrillar protein aggregates manifesting clinically in the anterior chamber of the eye. This disorder is the most commonly known cause of glaucoma and a major cause of irreversible blindness. Objective: To determine if exfoliation syndrome is associated with rare, protein-changing variants predicted to impair protein function. Design, Setting, and Participants: A 2-stage, case-control, whole-exome sequencing association study with a discovery cohort and 2 independently ascertained validation cohorts. Study participants from 14 countries were enrolled between February 1999 and December 2019. The date of last clinical follow-up was December 2019. Affected individuals had exfoliation material on anterior segment structures of at least 1 eye as visualized by slit lamp examination. Unaffected individuals had no signs of exfoliation syndrome. Exposures: Rare, coding-sequence genetic variants predicted to be damaging by bioinformatic algorithms trained to recognize alterations that impair protein function. Main Outcomes and Measures: The primary outcome was the presence of exfoliation syndrome. Exome-wide significance for detected variants was defined as P < 2.5 × 10-6. The secondary outcomes included biochemical enzymatic assays and gene expression analyses. Results: The discovery cohort included 4028 participants with exfoliation syndrome (median age, 78 years [interquartile range, 73-83 years]; 2377 [59.0%] women) and 5638 participants without exfoliation syndrome (median age, 72 years [interquartile range, 65-78 years]; 3159 [56.0%] women). In the discovery cohort, persons with exfoliation syndrome, compared with those without exfoliation syndrome, were significantly more likely to carry damaging CYP39A1 variants (1.3% vs 0.30%, respectively; odds ratio, 3.55 [95% CI, 2.07-6.10]; P = 6.1 × 10-7). This outcome was validated in 2 independent cohorts. The first validation cohort included 2337 individuals with exfoliation syndrome (median age, 74 years; 1132 women; n = 1934 with demographic data) and 2813 individuals without exfoliation syndrome (median age, 72 years; 1287 women; n = 2421 with demographic data). The second validation cohort included 1663 individuals with exfoliation syndrome (median age, 75 years; 587 women; n = 1064 with demographic data) and 3962 individuals without exfoliation syndrome (median age, 74 years; 951 women; n = 1555 with demographic data). Of the individuals from both validation cohorts, 5.2% with exfoliation syndrome carried CYP39A1 damaging alleles vs 3.1% without exfoliation syndrome (odds ratio, 1.82 [95% CI, 1.47-2.26]; P <.001). Biochemical assays classified 34 of 42 damaging CYP39A1 alleles as functionally deficient (median reduction in enzymatic activity compared with wild-type CYP39A1, 94.4% [interquartile range, 78.7%-98.2%] for the 34 deficient variants). CYP39A1 transcript expression was 47% lower (95% CI, 30%-64% lower; P <.001) in ciliary body tissues from individuals with exfoliation syndrome compared with individuals without exfoliation syndrome. Conclusions and Relevance: In this whole-exome sequencing case-control study, presence of exfoliation syndrome was significantly associated with carriage of functionally deficient CYP39A1 sequence variants. Further research is needed to understand the clinical implications of these findings.},
author = {Li, Zheng and Wang, Zhenxun and Lee, Mei Chin and Zenkel, Matthias and Peh, Esther and Ozaki, Mineo and Topouzis, Fotis and Nakano, Satoko and Chan, Anita and Chen, Shuwen and Williams, Susan E.I. and Orr, Andrew and Nakano, Masakazu and Kobakhidze, Nino and Zarnowski, Tomasz and Popa-Cherecheanu, Alina and Mizoguchi, Takanori and Manabe, Shin Ichi and Hayashi, Ken and Kazama, Shigeyasu and Inoue, Kenji and Mori, Yosai and Miyata, Kazunori and Sugiyama, Kazuhisa and Higashide, Tomomi and Chihara, Etsuo and Ideta, Ryuichi and Ishiko, Satoshi and Yoshida, Akitoshi and Tokumo, Kana and Kiuchi, Yoshiaki and Ohashi, Tsutomu and Sakurai, Toshiya and Sugimoto, Takako and Chuman, Hideki and Aihara, Makoto and Inatani, Masaru and Mori, Kazuhiko and Ikeda, Yoko and Ueno, Morio and Gaston, Daniel and Rafuse, Paul and Shuba, Lesya and Saunders, Joseph and Nicolela, Marcelo and Chichua, George and Tabagari, Sergo and Founti, Panayiota and Sim, Kar Seng and Meah, Wee Yang and Soo, Hui Meng and Chen, Xiao Yin and Chatzikyriakidou, Anthi and Keskini, Christina and Pappas, Theofanis and Anastasopoulos, Eleftherios and Lambropoulos, Alexandros and Panagiotou, Evangelia S. and Mikropoulos, Dimitrios G. and Kosior-Jarecka, Ewa and Cheong, Augustine and Li, Yuanhan and Lukasik, Urszula and Nongpiur, Monisha E. and Husain, Rahat and Perera, Shamira A. and Álvarez, Lydia and García, Montserrat and González-Iglesias, Héctor and Cueto, Andrés F.V. and Cueto, Luis F.V. and Martinón-Torres, Federico and Salas, Antonio and Oguz, Çilingir and Tamcelik, Nevbahar and Atalay, Eray and Batu, Bilge and Irkec, Murat and Aktas, Dilek and Kasim, Burcu and Astakhov, Yury S. and Astakhov, Sergei Y. and Akopov, Eugeny L. and Gießl, Andreas and Mardin, Christian Y. and Hellerbrand, Claus and Cooke Bailey, Jessica N. and Igo, Robert P. and Haines, Jonathan L. and Edward, Deepak P. and Heegaard, Steffen and Davila, Sonia and Tan, Patrick and Kang, Jae H. and Pasquale, Louis R. and Kruse, Friedrich and Reis, André and Carmichael, Trevor R. and Hauser, Michael and Ramsay, Michele and Mossböck, Georg and Yildirim, Nilgun and Tashiro, Kei and Konstas, Anastasios G.P. and Coca-Prados, Miguel and Foo, Jia Nee and Kinoshita, Shigeru and Sotozono, Chie and Kubota, Toshiaki and Dubina, Michael and Ritch, Robert and Wiggs, Janey L. and Pasutto, Francesca and Schlötzer-Schrehardt, Ursula and Ho, Ying Swan and Aung, Tin and Tam, Wai Leong and Khor, Chiea Chuen},
doi = {10.1001/jama.2021.0507},
faupublication = {yes},
journal = {Journal of the American Medical Association},
note = {CRIS-Team Scopus Importer:2021-03-05},
pages = {753-764},
peerreviewed = {Yes},
title = {{Association} of {Rare} {CYP39A1} {Variants} with {Exfoliation} {Syndrome} {Involving} the {Anterior} {Chamber} of the {Eye}},
volume = {325},
year = {2021}
}
@inproceedings{faucris.208827215,
author = {Komori, Yuya and Okumura, Naoki and Hayashi, Ryosuke and Nakano, Masakazu and Tashiro, Kei and Yoshii, Kengo and Aleff, Ross and Butz, Malinda and Highsmith, Edward W. and Wieben, Eric and Fautsch, Michael P. and Baratz, Keith H. and Tourtas, Theofilos and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich and Koizumi, Noriko},
faupublication = {yes},
note = {EVALuna2:34743},
peerreviewed = {Yes},
title = {{Association} of rs613872 and trinucleotide repeat expansion in the {TCF4} gene in {Fuchs} endothelial corneal dystrophy in {Germany}},
volume = {59},
year = {2018}
}
@article{faucris.221251037,
abstract = {PURPOSE: To investigate single nucleotide polymorphisms (SNPs) and trinucleotide repeat (TNR) expansion in the transcription factor 4 (TCF4) gene in a large cohort of German patients with Fuchs endothelial corneal dystrophy (FECD). METHODS: Genomic DNA was obtained from 398 patients with FECD and from 58 non-FECD controls. Thirty-seven previously reported SNPs were evaluated by genotyping. The 398 FECD samples were analyzed for TNR expansions by short tandem repeat assays and Southern blotting. The possible associations between the TNR length and clinical parameters (age, sex, visual acuity, and central corneal thickness) were analyzed in 132 patients. RESULTS: The SNPs in COL8A2, TCF8, LOXHD1, and AGBL1 showed no heterogeneity in 36 cases, although SLCA411 showed 3 nonsense mutations. SNPs were detected for TCF4 (rs613872, rs2123392, rs17089887, rs1452787, and rs1348047), but only rs613872 showed a significant association with FECD (P = 9.93 × 10). Overall, 315/398 (79%) patients harbored TNR lengths >50, whereas no non-FECD controls harbored TNR lengths >50. The TCF4 SNP rs613872 genotype was TT: 39 (67%), TG: 18 (31%), and GG: 1 (2%) in non-FECD controls; TT: 39 (47%), TG: 38 (46%), and GG: 6 (7%) in FECD cases harboring TNR <50; and TT: 23 (8%), TG: 224 (79%), and GG: 38 (13%) in FECD cases harboring TNR >50 (P = 2.93 × 10). No significant association was detected between the TNR length and clinical parameters. CONCLUSIONS: Our large German cohort demonstrated a significant association between the risk allele G in rs613872 and FECD, irrespective of TNR expansion, although this risk allele was more frequent in FECD cases with TNR expansion than without.},
author = {Okumura, Naoki and Hayashi, Ryousuke and Nakano, Masakazu and Tashiro, Kei and Yoshii, Kengo and Aleff, Ross and Butz, Malinda and Highsmith, Edward W. and Wieben, Eric D. and Fautsch, Michael P. and Baratz, Keith H. and Komori, Yuya and Ueda, Emi and Nakahara, Makiko and Weller, Julia and Tourtas, Theofilos and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich and Koizumi, Noriko},
doi = {10.1097/ICO.0000000000001952},
faupublication = {yes},
journal = {Cornea},
note = {CRIS-Team Scopus Importer:2019-06-25},
pages = {799-805},
peerreviewed = {Yes},
title = {{Association} of rs613872 and {Trinucleotide} {Repeat} {Expansion} in the {TCF4} {Gene} of {German} {Patients} {With} {Fuchs} {Endothelial} {Corneal} {Dystrophy}},
volume = {38},
year = {2019}
}
@article{faucris.108127184,
abstract = {Lamellar techniques for selective replacement of diseased corneal structures have recently been improved. Descemet membrane endothelial keratoplasty (DMEK) allows the sole replacement of the endothelium--Descemet membrane layer (EDM). However, wide-spread use of DMEK is currently limited because of problems with donor preparation namely the tearing of the Descemet membrane and the difficulty to unfold the EDM graft in the anterior chamber (AC).A standardized DMEK procedure that allows safe preparation of EDM, atraumatic introduction of EDM into the AC, reliable orientation of EDM during surgery, and stepwise unfolding within the AC is described in 80 patients. Visual acuity and corneal endothelial cell density were assessed.A stepwise approach using a novel bimanual underwater technique to harvest EDM from donor corneal buttons allows reproducible generation of grafts without tearing the Descemet membrane. Injection of the EDM roll into the AC is achieved by use of a standard injector cartridge, whereas the depth of AC is maintained by an irrigation handpiece. Marks at the margin of EDM allow orientation. Finally, unfolding EDM in the AC is achieved by sequential use of water jets and air bubbles. In the early phase of the learning curve, 4 patients were regrafted because of graft failure. Endothelial cell density decreased from 2600 6 252 to 1526 6 341 cells per square millimeter 1 month after DMEK.A novel technique for graft preparation and EDM injection results in improved safety with a high rate of successful DMEK},
author = {Kruse, Friedrich and Laaser, Kathrin and Cursiefen, Claus and Heindl, Ludwig M. and Schlötzer-Schrehardt, Ursula and Riss, Stephan and Bachmann, Bjoern O.},
doi = {10.1097/ICO.0b013e3182000e2e},
faupublication = {yes},
journal = {Cornea},
note = {EVALuna2:21013},
pages = {580-7},
peerreviewed = {Yes},
title = {{A} stepwise approach to donor preparation and insertion increases safety and outcome of {Descemet} membrane endothelial keratoplasty},
volume = {30},
year = {2011}
}
@article{faucris.124119204,
abstract = {The chemokine receptor CCR7 is essential for migration of mature dendritic cells (DCs) to the regional lymph nodes, and it has been shown that blocking of CCR7 improves graft survival after high-risk corneal transplantation in vascularized recipient corneas. However, it is so far unknown whether blocking of CCR7 reduces migration of DCs from the avascular cornea to the draining lymph nodes and whether this leads to improved graft survival also in the low-risk setting of corneal transplantation, which accounts for the majority of perforating transplantations performed. Therefore, in this study, pellets containing Freund's adjuvant and bovine serum albumin (BSA) conjugated to Alexa488 fluorescent dye were implanted into the corneal stroma of BALB/c mice to analyze antigen uptake by corneal DCs and their migration to the regional lymph nodes. After pellet implantation, mice were either treated by local administration of a CCR7 blocking fusion protein that consisted of CCL19 fused to the Fc part of human IgG1 or a control-IgG. In vivo fluorescence microscopy showed uptake of Alexa488-conjugated BSA by corneal DCs within 8 h. Furthermore, analysis of single cell suspensions of draining lymph nodes prepared after 48 h revealed that 2.1 ± 0.3% of CD11c(+) cells were also Alexa488(+). Importantly, DC migration was significantly reduced after topical administration of CCL19-IgG (1.2 ± 0.2%; p < 0.05). To test the effect of CCR7 blockade on graft rejection after allogeneic low-risk keratoplasty, corneal transplantations were performed using C57BL/6-mice as donors and BALB/c-mice as recipients. Treatment mice received two intraperitoneal loading doses of CCL19-IgG prior to transplantation, followed by local treatment with CCL19-IgG containing eye drops for the first two weeks after transplantation. Control mice received same amounts of control-IgG. Kaplan-Meier survival analysis showed that in the CCL19-IgG treated group, 76% of the grafts survived through the end of the 8 week observation period, whereas 38% of the grafts survived in the control group (p < 0.05). Taken together, our study shows that blockade of CCR7 reduces the migration of mature corneal DCs to the draining lymph nodes and leads to improved graft survival in low-risk corneal transplantation.},
author = {Hos, D. and Dörrie, Jan and Schaft, Niels and Bock, F. and Notara, M. and Kruse, Friedrich and Krautwald, S. and Cursiefen, C. and Bachmann, B. O.},
doi = {10.1016/j.exer.2015.12.004},
faupublication = {yes},
journal = {Experimental Eye Research},
note = {EVALuna2:19600},
pages = {1-6},
peerreviewed = {Yes},
title = {{Blockade} of {CCR7} leads to decreased dendritic cell migration to draining lymph nodes and promotes graft survival in low-risk corneal transplantation},
volume = {146},
year = {2016}
}
@article{faucris.106700044,
abstract = {To evaluate the impact of the air bubble on endothelial cell loss using the "bubble-in-the-roll" technique during Descemet membrane endothelial keratoplasty (DMEK).Twenty DMEK grafts not suitable for transplantation were manually prepared from organ-cultured corneoscleral discs and injected into culture media using the Endoject DMEK injector (Medicel AG, Wolfhalden, Switzerland). Based on the injection method, the grafts were divided into 2 groups: In group A (n = 10), a small air bubble was placed inside the graft roll while it was in the injector. In group B (n = 10), the grafts were injected without an air bubble inside the graft roll. Main outcome measures included endothelial cell density (ECD) after graft stripping and graft injection.There were no statistically significant differences between groups A and B in donor age, storage duration, and donor ECD. ECD decreased from 1929 ± 145 cells/mm to 1796 ± 303 cells/mm after graft stripping in group A and from 1801 ± 226 cells/mm to 1709 ± 290 cells/mm in group B. ECD after graft injection further decreased to 1683 ± 291 cells/mm in group A and to 1651 ± 292 cells/mm in group B. Endothelial cell loss after graft stripping and graft injection was not statistically significant between groups A and B (P = 0.29 and P = 1, respectively).The bubble-in-the-roll technique for injection and unfolding of the graft is a safe method for graft delivery into the anterior chamber guaranteeing orientation of the graft without harming the endothelium.},
author = {Akbaba, Yasemin and Weller, Julia M. and Rössler, Kathrin and Armitage, W. John and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich and Tourtas, Theofilos},
doi = {10.1097/ICO.0000000000001360},
faupublication = {yes},
journal = {Cornea},
note = {EVALuna2:21346},
pages = {1576-1579},
peerreviewed = {Yes},
title = {"{Bubble}-in-the-{Roll}" {Technique} {Using} the {Endoject} {DMEK} {Injector}: {Influence} of the {Air} {Bubble} on {Endothelial} {Cell} {Loss}},
volume = {36},
year = {2017}
}
@inproceedings{faucris.208826986,
author = {Augustin, Victor A. and Weller, Julia and Kruse, Friedrich and Tourtas, Theofilos},
faupublication = {yes},
note = {EVALuna2:34740},
peerreviewed = {Yes},
title = {{Can} we optimize the refractive target in triple {Descemet} membrane endothelial keratoplasty in the fellow eye after urgery in the first eye?},
volume = {59},
year = {2018}
}
@article{faucris.215693759,
abstract = {Purpose: To analyze and correlate corneal parameters with refractive shift after Descemet membrane endothelial keratoplasty combined with cataract surgery (triple Descemet membrane endothelial keratoplasty). Methods: This single-center retrospective observational case series included 152 eyes of 152 consecutive patients undergoing triple Descemet membrane endothelial keratoplasty in the first eye for Fuchs endothelial corneal dystrophy. Patients were examined preoperatively, as well as at 3, 6, and 12 months after surgery. The main outcome measures were: refractive shift (predicted refractive outcome based on intraocular lens calculation compared to actual postoperative refractive outcome), central corneal thickness, corneal volume, anterior and posterior corneal curvature, and corneal densitometry. These parameters were analyzed and correlated with the refractive shift after surgery. Results: After 3 months from surgery, a mean refractive shift of +1.12 +/- 1.10 D was observed and remained stable until the last follow-up at 12 months (+1.24 +/- 1.07 D). Correlation analysis showed a weak but significant positive correlation between refractive shift and preoperative posterior curvature (rho = 0.314; p = 0.002) or preoperative posterior densitometry (rho = 0.227; p = 0.008). No correlation was found between refractive shift and preoperative central corneal thickness, corneal volume, anterior curvature, or anterior/mid-cornea densitometry. Conclusion: Changes of the posterior cornea may have an influence on the refractive shift. Patients with flatter posterior corneal curvature or higher posterior corneal density seem to exhibit a higher hyperopic shift. The weak correlations indicate a poor predictive value of any preoperative parameter used in our study.},
author = {Augustin, Victor A. and Weller, Julia M. and Kruse, Friedrich and Tourtas, Theofilos},
doi = {10.1177/1120672118785282},
faupublication = {yes},
journal = {European Journal of Ophthalmology},
note = {CRIS-Team WoS Importer:2019-04-09},
pages = {165-170},
peerreviewed = {Yes},
title = {{Can} we predict the refractive outcome after triple {Descemet} membrane endothelial keratoplasty?},
volume = {29},
year = {2019}
}
@article{faucris.267252621,
abstract = {Clinical features of Coronavirus disease 2019 (COVID-19) are caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Acute infection management is a substantial healthcare issue, and the development of long-Covid syndrome (LCS) is extremely challenging for patients and physicians. It is associated with a variety of characteristics as impaired capillary microcirculation, chronic fatigue syndrome (CFS), proinflammatory cytokines, and functional autoantibodies targeting G-protein-coupled receptors (GPCR-AAbs). Here, we present a case report of successful healing of LCS with BC 007 (Berlin Cures, Berlin, Germany), a DNA aptamer drug with a high affinity to GPCR-AAbs that neutralizes these AAbs. A patient with a documented history of glaucoma, recovered from mild COVID-19, but still suffered from CFS, loss of taste, and impaired capillary microcirculation in the macula and peripapillary region. He was positively tested for various targeting GPCR-AAbs. Within 48 h after a single BC 007 treatment, GPCR-AAbs were functionally inactivated and remained inactive during the observation period of 4 weeks. This observation was accompanied by constant improvement of the fatigue symptoms of the patient, taste, and retinal capillary microcirculation. Therefore, the removal of GPCR-AAb might ameliorate the characteristics of the LCD, such as capillary impairment, loss of taste, and CFS.},
author = {Hohberger, Bettina and Harrer, Thomas and Mardin, Christian and Kruse, Friedrich and Hoffmanns, Jakob and Rogge, Lennart and Heltmann, Felix and Moritz, Michael and Szewczykowski, Charlotte and Schottenhamml, Julia and Kräter, Martin and Bergua, Antonio and Zenkel, Matthias and Gießl, Andreas and Schlötzer-Schrehardt, Ursula and Lämmer, Robert and Herrmann, Martin and Haberland, Annekathrin and Göttel, Peter and Müller, Johannes and Wallukat, Gerd},
doi = {10.3389/fmed.2021.754667},
faupublication = {yes},
journal = {Frontiers in Medicine},
keywords = {BC 007; chronic fatigue syndrome; COVID-19; functional GPCR autoantibodies; glaucoma; long-COVID syndrome; OCT angiography},
note = {CRIS-Team Scopus Importer:2021-12-17},
peerreviewed = {Yes},
title = {{Case} {Report}: {Neutralization} of {Autoantibodies} {Targeting} {G}-{Protein}-{Coupled} {Receptors} {Improves} {Capillary} {Impairment} and {Fatigue} {Symptoms} {After} {COVID}-19 {Infection}},
volume = {8},
year = {2021}
}
@article{faucris.114208864,
abstract = {Interactions between stem cells and their microenvironment are critical for regulation and maintenance of stem cell function. To elucidate the molecular interactions within the human limbal epithelial stem/progenitor cell (LEPC) niche, which is essential for maintaining corneal transparency and vision, we performed a comprehensive expression analysis of cell adhesion molecules (CAMs) using custom-made quantitative real-time polymerase chain reaction (qRT-PCR) arrays and laser capture-microdissected LEPC clusters, comprising LEPCs, melanocytes, mesenchymal cells, and transmigrating immune cells. We show that LEPCs are anchored to their supporting basement membrane by the laminin receptors ?3?1 and ?6?4 integrin and the dystroglycan complex, while intercellular contacts between LEPCs and melanocytes are mediated by N-, P-, and E-cadherin together with L1-CAM, a member of the immunoglobulin superfamily (Ig)CAMs. In addition to the LEPC-associated heparan sulfate proteoglycans syndecan-2, glypican-3, and glypican-4, the IgCAM members ICAM-1 and VCAM-1 were found to be variably expressed on LEPCs and associated niche cells and to be dynamically regulated in response to chemokines such as interferon-? to enhance interactions with immune cells. Moreover, junctional adhesion molecule JAM-C accumulating in the subepithelial limbal matrix, appeared to be involved in recruitment of immune cells, while mesenchymal stromal cells appeared to use the nephronectin receptor integrin ?8 for approaching the limbal basement membrane. In summary, we identified a novel combination of cell surface receptors that may regulate both stable and dynamic cell-matrix and cell-cell interactions within the limbal niche. The findings provide a solid foundation for further functional studies and for advancement of our current therapeutic strategies for ocular surface reconstruction. Stem Cells 2016;34:203-219.},
author = {Polisetti, Naresh and Zenkel, Matthias and Menzel-Severing, Johannes and Kruse, Friedrich and Schlötzer-Schrehardt, Ursula},
doi = {10.1002/stem.2191},
faupublication = {yes},
journal = {Stem Cells},
note = {EVALuna2:21232},
pages = {203-19},
peerreviewed = {Yes},
title = {{Cell} {Adhesion} {Molecules} and {Stem} {Cell}-{Niche}-{Interactions} in the {Limbal} {Stem} {Cell} {Niche}},
volume = {34},
year = {2016}
}
@article{faucris.297267479,
abstract = {Background/aims: Ectasia of the cornea can occur decades after penetrating keratoplasty (PK), especially in keratoconus eyes. The purpose of this study was to characterise ectasia after PK by morphological findings in anterior segment optical coherence tomography (AS-OCT). Methods: In this retrospective, single-centre case series, 50 eyes of 32 patients with a history of PK at an average of 25±10 years earlier were included. The eyes were classified either as ectatic (n=35) or as non-ectatic (n=15). The main parameters included central corneal thickness (CCT), lowest corneal thickness at the interface (LCTI), anterior chamber depth, graft-host interface angle at the thinnest point and host cornea-iris angle. Furthermore, steep and flat keratometry readings obtained by AS-OCT (CASIA-2, Tomey) and Scheimpflug tomography (Pentacam, Oculus) were assessed. OCT findings were correlated with clinical grading of ectasia. Results: There was a highly significant difference in LCTI, graft-host interface angle and anterior chamber depth (in pseudophakic eyes) between the groups. The ratio calculated by the quotient of LCTI divided by CCT was significantly lower in ectatic than non-ectatic eyes (p<0.001). In eyes with an LCTI/CCT ratio of ≤0.7, the OR for the occurrence of a clinical detectable ectasia was 2.4 (CI 1.5 to 3.7). Steep keratometry values were significantly higher in ectatic eyes. Conclusion: AS-OCT is a helpful tool to recognise and quantify ectasia in post-PK eyes objectively.},
author = {Weller, Julia and Hübner, Lisa and Kruse, Friedrich and Tourtas, Theofilos},
doi = {10.1136/bjo-2022-322859},
faupublication = {yes},
journal = {British Journal of Ophthalmology},
keywords = {Cornea; Imaging; Treatment Surgery},
note = {CRIS-Team Scopus Importer:2023-04-21},
peerreviewed = {Yes},
title = {{Characterisation} of ectasia after penetrating keratoplasty in keratoconus eyes using anterior segment optical coherence tomography},
year = {2023}
}
@article{faucris.119764084,
abstract = {To define the cleavage plane between Descemet's membrane (DM) and posterior corneal stroma in Descemet's membrane endothelial keratoplasty (DMEK) concerning its ultrastructural and immunohistochemical characteristics.Observational, consecutive case series.Fifteen corneoscleral buttons from donors 71.5±4.3 years of age stored in Optisol-GS and used for DMEK surgery in 15 consecutive patients.Endothelial cell-DM complexes (EDMs) and corresponding corneoscleral rims were investigated by transmission electron microscopy and immunohistochemistry using a panel of antibodies against adhesive matrix proteins.Ultrastructural and immunohistochemical characteristics of interface and cleavage plane between DM and posterior stroma.Connection between DM and corneal stroma was mediated predominantly by amorphous material of the interfacial matrix and projecting stromal collagen fibers. After DM stripping, the cleavage plane was located consistently between interfacial matrix and posterior stromal collagen lamellae, providing a largely smooth anterior EDM surface exposing the interfacial zone. Interindividual variations in amount and composition of the interfacial matrix resulted in variable degrees of EDM surface irregularities and variable staining patterns for adhesive matrix proteins such as fibronectin, vitronectin, amyloid P, osteonectin/secreted protein acidic and rich in cysteine (SPARC), fibulin-1, fibulin-2, fibulin-3, fibrillin-1, and keratoepithelin.The findings provide evidence for the existence of a physiologic cleavage plane between the interfacial matrix, the anteriormost adhesive zone of DM, and the corneal stroma, suggesting a relatively weak attachment that can be disconnected by mechanical forces. Interindividual variations in structure and composition of the interfacial matrix may provide an explanation for the variable attachment of EDM grafts to the recipients' corneal stroma and thus may affect the postoperative clinical outcome.The author(s) have no proprietary or commercial interest in any materials discussed in this article.},
author = {Schlötzer-Schrehardt, Ursula and Bachmann, Bjoern O. and Laaser, Kathrin and Cursiefen, Claus and Kruse, Friedrich},
doi = {10.1016/j.ophtha.2011.03.025},
faupublication = {yes},
journal = {Ophthalmology},
note = {EVALuna2:21042},
pages = {1950-7},
peerreviewed = {Yes},
title = {{Characterization} of the cleavage plane in {DESCemet}'s membrane endothelial keratoplasty},
volume = {118},
year = {2011}
}
@article{faucris.109598764,
abstract = {To evaluate the role of preexisting corneal pathology on the outcome of Descemet membrane endothelial keratoplasty (DMEK), and also to evaluate the long-term outcome of repeat DMEK for graft failure after primary DMEK.Eighteen patients undergoing repeat DMEK after failed DMEK were enrolled; 9 of 18 patients had successful primary DMEK on the fellow eye. Evaluations included preoperative anterior chamber depth, intraoperative degree of difficulty, transmission electron microscopy images (n = 14), best-corrected visual acuity (BCVA), endothelial cell density, central corneal thickness, corneal volume, and patient satisfaction.Surgeries that led to graft failure had a higher intraoperative degree of difficulty compared with successful surgeries (P = 0.002). Eight of 14 failed grafts showed ultrastructural abnormalities, that is, inclusions or deposits of abnormal fibrillar material in Descemet membrane, indicating endothelial dysfunction before transplantation. BCVA on day 10 after surgery was worse in eyes with graft failure compared with successful DMEK (P = 0.008). Median BCVA (logarithm of the minimum angle of resolution) improved from 0.5 before DMEK and 1.9 before repeat DMEK to 0.3 at 1-year follow-up (P = 0.011). One year after repeat DMEK, endothelial cell density (cells/mm) of donor corneas decreased from 2501 ± 264 to 1373 ± 270 (P < 0.001), central corneal thickness (µm) decreased from 807 ± 160 to 576 ± 178 (P = 0.002), and corneal volume (mm) decreased from 84.1 ± 13.0 to 64.4 ± 12.5 (P = 0.002). Patient satisfaction showed no difference between primary and repeat DMEK.A preexisting subclinical corneal endothelial dysfunction may contribute to primary DMEK failure. Repeat DMEK can be performed safely with good long-term outcome.},
author = {Cirkovic, Aleksandar and Schlötzer-Schrehardt, Ursula and Weller, Julia M. and Kruse, Friedrich and Tourtas, Theofilos},
doi = {10.1097/ICO.0000000000000295},
faupublication = {yes},
journal = {Cornea},
note = {EVALuna2:21222},
pages = {11-7},
peerreviewed = {Yes},
title = {{Clinical} and {Ultrastructural} {Characteristics} of {Graft} {Failure} in {DMEK}: 1-{Year} {Results} {After} {Repeat} {DMEK}},
volume = {34},
year = {2015}
}
@article{faucris.276327880,
author = {Bonnet, Clémence and Tseng, Chi Hong and Kruse, Friedrich and Deng, Sophie X.},
doi = {10.1016/j.ajo.2022.01.027},
faupublication = {yes},
journal = {American Journal of Ophthalmology},
note = {CRIS-Team Scopus Importer:2022-06-03},
pages = {202-203},
peerreviewed = {Yes},
title = {{Comment} on: {Long}-{Term} {Outcome} {After} {Superficial} {Keratectomy} of the {Abnormal} {Epithelium} for {Partial} {Limbal} {Stem} {Cell} {Deficiency}},
volume = {238},
year = {2022}
}
@article{faucris.119641324,
abstract = {: Human amniotic membrane has been widely used as substrate for ex vivo expansion and transplantation of limbal epithelial cells. To further clarify its suitability as a surrogate niche for limbal stem cells and progenitor cells, we analyzed the composition of the amniotic epithelial basement membrane, with special focus on the expression of limbus-specific matrix components.: Cryosections of corneoscleral specimens obtained from 10 human donor eyes and of 6 amniotic membrane specimens obtained at cesarean section were stained by indirect immunofluorescence using a broad panel of antibodies against basement membrane components.: Both amniotic and limbal epithelial basement membranes showed positive immunoreactivity for collagen type IV ?1, ?2, ?5, and ?6 chains; collagens type VII, XV, XVI, XVII, and XVIII; laminin ?3, ?1, ?2, ?3, ?1, and ?2 chains; laminin-111 and laminin-332; nidogen-1 and nidogen-2; fibronectin; fibulin-2; fibrillin-2; perlecan; and agrin. Both types of basement membrane were negative for collagen type IV ?3 and ?4 chains, collagen type V, and laminin ?4 chain. Limbal basement membrane components, which were not detected in amniotic membrane, included laminin ?1, ?2, ?5, and ?3 chains; BM40/SPARC; tenascin-C; matrilin-2; endostatin; and collagen type XVIII.: Despite extensive similarities in basement membrane composition between amniotic and corneolimbal epithelia, the lack of limbus-specific environmental factors argues against the potential of denuded amniotic membrane as a surrogate niche for limbal stem cells but supports its suitability as a substrate to promote the formation of a well-differentiated stratified corneal epithelial equivalent for tissue engineering strategies.},
author = {Dietrich-Ntoukas, Tina and Hofmann-Rummelt, Carmen and Kruse, Friedrich and Schlötzer-Schrehardt, Ursula},
doi = {10.1097/ICO.0b013e3182254b78},
faupublication = {yes},
journal = {Cornea},
note = {EVALuna2:21080},
pages = {564-9},
peerreviewed = {Yes},
title = {{Comparative} analysis of the basement membrane composition of the human limbus epithelium and amniotic membrane epithelium},
volume = {31},
year = {2012}
}
@article{faucris.111147344,
abstract = {To compare the diagnostic value of Bruch's membrane opening minimum rim width (BMO-MRW) and retinal nerve fiber layer thickness (RNFLT) in patients with ocular hypertension, preperimetric, and perimetric glaucoma.One hundred eighty-one eyes consisting of 40 healthy controls, 41 ocular hypertensive subjects, 50 subjects with preperimetric glaucoma and 50 with perimetric glaucoma were included. One randomly selected eye was included. All patients underwent slit-lamp examination, funduscopy, achromatic perimetry, and 24-hour IOP profile. Bruch's membrane opening-MRW and RNFLT (3 peripapillary circle scans, 12°/14°/16°) data were obtained using spectral domain optical coherence tomography. Areas under the receiver operating characteristics curves (AUROC) as well as sensitivity at fixed specificity were computed globally and for six vertical split sectors. Venn diagrams were plotted to identify patients that were diagnosed by one of the two parameters only.For RNFLT the smallest circle diameter showed highest diagnostic accuracy and was used for comparison with BMO-MRW. Distinguishing perimetric glaucoma, RNFLT and BMO-MRW showed comparable AUROCs in global (AUROC, 95% confidence interval: 0.954, 0.911-0.996 and 0.929, 0.872-0.986) and sectoral (0.929, 0.877-0.981 and 0.946, 0.904-0.996) analysis. For preperimetric glaucoma BMO-MRW and RNFLT also demonstrated comparable performance in global (0.839, 0.757-0.921 and 0.821, 0.731-0.912) and sectoral (0.860, 0.782-0.938 and 0.835, 0.750-0.920) analysis. When identifying ocular hypertensive eyes AUROCs were lower for global RNFLT and BMO-MRW (0.493, 0.365-0.621 and 0.562, 0.433-0.691). A combined parameter showed an AUROC of 0.959, 0.921 to 0.996 for perimetric and 0.849, 0.770 to 0.929 for preperimetric glaucoma.Bruch's membrane opening-MRW and RNFLT are comparably useful parameters for discrimination of preperimetric and perimetric glaucomatous eyes and show potential to assist each other in glaucoma diagnosis. (www.ClinicalTrials.gov number, NTC00494923; Erlangen Glaucoma Registry.).},
author = {Gmeiner, Jonas M. D. and Schrems, Wolfgang and Mardin, Christian Y. and Lämmer, Robert and Kruse, Friedrich and Schrems-Hösl, Laura-Maria},
doi = {10.1167/iovs.15-18906},
faupublication = {yes},
journal = {Investigative Ophthalmology & Visual Science},
note = {EVALuna2:21294},
pages = {OCT575-84},
peerreviewed = {Yes},
title = {{Comparison} of {Bruch}'s {Membrane} {Opening} {Minimum} {Rim} {Width} and {Peripapillary} {Retinal} {Nerve} {Fiber} {Layer} {Thickness} in {Early} {Glaucoma} {Assessment}},
volume = {57},
year = {2016}
}
@article{faucris.111080684,
abstract = {To compare Moorfields regression analysis (MRA), Glaucoma probability score (GPS), and different discriminant functions to predict future visual field conversion of patients with ocular hypertension and early glaucoma.The study included 120 eyes of patients with ocular hypertension and 110 eyes of patients with early glaucoma from the Erlangen glaucoma registry. Annually, all patients underwent standard automated perimetry, 24-hour intraocular pressure profile, optic disc photography, and HRT (Heidelberg Retina Tomograph I-III; Heidelberg Engineering) measurements. The cohort was divided into 2 groups based on the development of repeatable glaucomatous visual fields. Positive predictive values and negative predictive values were compared for MRA, GPS, and the classification of Bathija, Iester, Mardin, and Mikelberg at baseline. Kaplan-Meier Survival curves and Logrank tests were used to evaluate equality of survival distributions for different test results.Median follow-up was 9.04 years. 26 eyes (11.3%) demonstrated glaucomatous visual field loss in the follow-up. MRA temporal-superior and temporal-inferior outside normal limits were predictive of future visual field loss with positive predictive values of 33.3% and 28.6%. Normal GPS Temporal Sector demonstrated a negative predictive value of 96.4% and normal results in discriminant functions between 94.7% and 95.5%.Confocal scanning laser tomography is a useful imaging modality to predict future visual field conversion. Development of visual field defects in 10 years is highly unlikely, if GPS classification and/or classification of discriminant analysis at baseline are normal. MRA temporal-superior and temporal-inferior outside normal limits are associated with future VF conversion (ClinicalTrials.gov number, NTC00494923).},
author = {Schrems-Hösl, Laura-Maria and Schrems, Wolfgang and Lämmer, Robert and Horn, Folkert and Jünemann, Anselm and Kruse, Friedrich and Mardin, Christian Y.},
doi = {10.1097/IJG.0000000000000171},
faupublication = {yes},
journal = {Journal of Glaucoma},
note = {EVALuna2:21172},
pages = {371-6},
peerreviewed = {Yes},
title = {{Confocal} {Laser} {Scanning} {Tomography} to {Predict} {Visual} {Field} {Conversion} in {Patients} {With} {Ocular} {Hypertension} and {Early} {Glaucoma}},
volume = {25},
year = {2016}
}
@inproceedings{faucris.223405363,
author = {Fuchsluger, Thomas and Thieme, Daniel and Kruse, Friedrich and Mahajan, Siddharth and Schubert, Dirk W.},
booktitle = {The Association for Research in Vision and Ophthalmology (ARVO)},
date = {2018-04-28/2018-05-03},
faupublication = {yes},
keywords = {corneal, ani-Bak},
peerreviewed = {Yes},
title = {{Corneal} endothelium can be protected against cell by anti-{Bax} {siRNA}},
venue = {Honolulo, Hawaii},
year = {2018}
}
@inproceedings{faucris.208826528,
author = {Fuchsluger, Thomas and Thieme, Daniel and Kruse, Friedrich and Mahajan, Siddharth},
faupublication = {yes},
note = {EVALuna2:34735},
peerreviewed = {Yes},
title = {{Corneal} endothelium can be protected against cell death by anti-{Bax} and anti-{Bak} {siRNA}},
volume = {59},
year = {2018}
}
@article{faucris.119641764,
abstract = {We compared corneal higher-order aberrations (HOAs) in eyes after Descemet's membrane endothelial keratoplasty (DMEK), Descemet's stripping automated endothelial keratoplasty (DSAEK), and penetrating keratoplasty (PK), and in a control group that had not undergone surgery.Retrospective analysis of clinical data.Thirty eyes of 30 patients who had undergone standard DMEK, 20 eyes of 20 patients after DSAEK, 20 eyes of 20 patients after PK, and 20 eyes of 20 controls were analyzed.In addition to standard postoperative examinations, each participant was analyzed with the Pentacam high-resolution rotating Scheimpflug imaging system (Pentacam HR, Oculus, Wetzlar, Germany). Data were compared between groups.Visual acuity and HOAs.The mean follow-up was 6.5±1.2 months after DMEK, 22.6±11.8 months after DSAEK, and 103.1±74.2 months after PK. There were no statistically significant differences for the anterior 4.0-mm zones between the DMEK group and the controls or between the DMEK and DSAEK groups. The DMEK procedure compared with PK showed statistically significant differences in all terms for the 4.0-mm zones. All combined Zernike terms for mean posterior aberrations of the central 4.0-mm zones showed statistically significant higher aberrations for DMEK compared with controls. The DMEK procedure compared with DSAEK showed statistically significant lower mean values for all combined Zernike terms, except for coma and coma-like terms in the central 4.0-mm zones of the posterior corneal surface. Compared with PK, DMEK showed statistically significant lower mean values for all combined Zernike terms for the central 4.0-mm zones of the posterior corneal surface, except for spherical aberration (SA) and SA-like terms. Best spectacle-corrected visual acuity (BSCVA) after DMEK was statistically significantly better than after DSAEK (P=0.001) and PK (P=0.005). There was no statistically significant difference when BSCVA was compared with controls (P=0.998).Both DSAEK and PK exhibit increased posterior corneal HOAs even years after surgery. Patients receiving DMEK display only slight changes in posterior corneal HOAs.Proprietary or commercial disclosure may be found after the references.},
author = {Rudolph, Michael and Laaser, Kathrin and Bachmann, Bjoern O. and Cursiefen, Claus and Epstein, Daniel and Kruse, Friedrich},
doi = {10.1016/j.ophtha.2011.08.034},
faupublication = {yes},
journal = {Ophthalmology},
note = {EVALuna2:21073},
pages = {528-35},
peerreviewed = {Yes},
title = {{Corneal} {Higher}-{Order} {Aberrations} after {Descemet}'s {Membrane} {Endothelial} {Keratoplasty}},
volume = {119},
year = {2012}
}
@article{faucris.109749904,
abstract = {To assess the suitability of a new 345 nm ultraviolet (UV) femtosecond laser for refractive surgery.Department of Ophthalmology, University of Erlangen-Nürnberg, Erlangen, Germany.Experimental study.Twenty-five porcine corneas were used for stromal flap or lamellar bed creation (stromal depth, 150 ?m) and 15 rabbit corneas for lamellar bed creation near the endothelium. Ultraviolet femtosecond laser cutting-line morphology, gas formation, and keratocyte death rate were evaluated using light and electron microscopy and compared with a standard infrared (IR) femtosecond laser. Endothelial cell survival was examined after application of a laser cut near the endothelium.Flaps created by the UV laser were lifted easily. Gas formation was reduced 4.2-fold compared with the IR laser (P = .001). The keratocyte death rate near the interface was almost doubled; however, the death zone was confined to a region within 38 ?m ± 10 (SD) along the cutting line. Histologically and ultrastructurally, a distinct and continuous cutting line was not found after UV femtosecond laser application if flap lifting was omitted and standard energy parameters were used. Instead, a regular pattern of vertical striations, presumably representing self-focusing induced regions of optical tissue breakdown, were identified. Lamellar bed creation with standard energy parameters 50 ?m from the endothelium rendered the endothelial cells intact and viable.The new 345 nm femtosecond laser is a candidate for pending in vivo trials and future high-precision flap creation, intrastromal lenticule extraction, and ultrathin Descemet-stripping endothelial keratoplasty.Mr. Klenke and Ms. Skerl were paid employees of Wavelight GmbH when the study was performed. Dr. Seiler is a scientific consultant to Wavelight GmbH. No other author has a financial or proprietary interest in any material or method mentioned.},
author = {Hammer, Christian M. and Petsch, Corinna and Klenke, Joerg and Skerl, Katrin and Paulsen, Friedrich and Kruse, Friedrich and Seiler, Theo and Menzel-Severing, Johannes},
doi = {10.1016/j.jcrs.2014.11.046},
faupublication = {yes},
journal = {Journal of Cataract and Refractive Surgery},
note = {EVALuna2:21240},
pages = {1279-88},
peerreviewed = {Yes},
title = {{Corneal} tissue interactions of a new 345 nm ultraviolet femtosecond laser},
volume = {41},
year = {2015}
}
@article{faucris.119786524,
abstract = {In recent years many three-dimensional cornea models have been developed. However, they show poor collagen stability in the stroma. Transglutaminases (Tgases) are calcium-dependent proteins which play an important role in cross-linking of the corneal stroma. The purpose of this study was to find out whether it is possible to induce in vitro cross-linking of the stroma in an artificial hemicornea model with the help of Tgases.For the construction of the hemicornea, human SV40 adenovector corneal epithelial cells (HCE) and human SV40 adenovector corneal keratocytes (HCK) were cultivated. Confluent HCK cells were treated for 24 h with transforming growth factor beta (TGFb) 1, 2 and 3 at different concentrations as well as with other growth factors and the treated cells were compared to untreated cultivated cells. The quantification of the expression of the Tgases by HCKs was examined with the use of real time PCR, Western blot imaging and immunochemistry.All concentrations of TGFbs used resulted in a significant increase of Tgase-mRNA, Tgase protein level and Tgase activity. The Tgases remained unaffected after treatment with other growth factors in comparison to untreated control cells. Treatment of the hemicornea with TGFb2 showed a very strong contraction and haze in comparison to the untreated hemicornea.It has been shown for the first time that TGFb induces a strong expression of Tgases in HCK cells. This effect caused an undesired contraction and haze of the human hemicornea model. Further research is necessary in order to find out whether the induction of Tgases in the HCK cells can be regulated without losing stability of the constructed hemicornea.},
author = {Kopsachilis, N. and Tsinopoulos, I. and Tsaousis, Konstantinos and Meiller, R. and Dimitrakos, S. A. and Kruse, Friedrich and Luessen, U. W.},
doi = {10.1007/s00347-012-2538-7},
faupublication = {yes},
journal = {Ophthalmologe},
note = {EVALuna2:21093},
pages = {583-90},
peerreviewed = {Yes},
title = {{Cross}-linking in an artificial human cornea via induction of tissue transglutaminases},
volume = {109},
year = {2012}
}
@inproceedings{faucris.265058308,
address = {ROCKVILLE},
author = {Narimoto, Kaito and Okumura, Naoki and Yamada, Shohei and Okamura, Kengo and Izumi, Ayaka and Tourtas, Theofilos and Kruse, Friedrich and Koizumi, Noriko},
booktitle = {INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE},
faupublication = {yes},
note = {CRIS-Team WoS Importer:2021-10-15},
peerreviewed = {unknown},
publisher = {ASSOC RESEARCH VISION OPHTHALMOLOGY INC},
title = {{Deep} neural network by transfer learning for the analysis of guttae in the patients with {Fuchs} endothelial corneal dystrophy},
year = {2021}
}
@article{faucris.247294968,
abstract = {Over the past decades, the number of patients with dry eye disease (DED) has increased dramatically. The incidence of DED is higher in Asia than in Europe and North America, suggesting the involvement of cultural or racial factors in DED etiology. Although many definitions of DED have been used, discrepancies exist between the various definitions of dry eye disease (DED) used across the globe. This article presents a clinical consensus on the definition of DED, as formulated in four meetings with global DED experts. The proposed new definition is as follows: “Dry eye is a multifactorial disease characterized by a persistently unstable and/or deficient tear film (TF) causing discomfort and/or visual impairment, accompanied by variable degrees of ocular surface epitheliopathy, inflammation and neurosensory abnormalities.” The key criteria for the diagnosis of DED are unstable TF, inflammation, ocular discomfort and visual impairment. This definition also recommends the assessment of ocular surface epitheliopathy and neurosensory abnormalities in each patient with suspected DED. It is easily applicable in clinical practice and should help practitioners diagnose DED consistently. This consensus definition of DED should also help to guide research and clinical trials that, to date, have been hampered by the lack of an established surrogate endpoint.},
author = {Tsubota, Kazuo and Pflugfelder, Stephen C. and Liu, Zuguo and Baudouin, Christophe and Kim, Hyo Myung and Messmer, Elisabeth M. and Kruse, Friedrich and Liang, Lingyi and Carreno-Galeano, Jimena Tatiana and Rolando, Maurizio and Yokoi, Norihiko and Kinoshita, Shigeru and Dana, Reza},
doi = {10.3390/ijms21239271},
faupublication = {yes},
journal = {International Journal of Molecular Sciences},
keywords = {Corneal epitheliopathy; Definition; Dry eye; Dry eye signs; Dry eye symptoms; Inflammation; Neuropathic pain; Tear film breakup},
note = {CRIS-Team Scopus Importer:2020-12-25},
pages = {1-24},
peerreviewed = {Yes},
title = {{Defining} dry eye from a clinical perspective},
volume = {21},
year = {2020}
}
@inproceedings{faucris.228448322,
address = {ROCKVILLE},
author = {Augustin, Victor A. and Weller, Julia M. and Kruse, Friedrich and Tourtas, Theofilos},
booktitle = {INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE},
date = {2019-04-28/2019-05-02},
faupublication = {yes},
note = {CRIS-Team WoS Importer:2019-10-29},
peerreviewed = {unknown},
publisher = {ASSOC RESEARCH VISION OPHTHALMOLOGY INC},
title = {{Delayed} corneal clearance after uneventful {Descemet} {Membrane} endothelial keratoplasty},
venue = {Vancouver, CANADA},
year = {2019}
}
@article{faucris.268131569,
abstract = {Purpose This study aimed to evaluate the clinical outcomes up to 10 years after Descemet membrane endothelial keratoplasty (DMEK). Methods In this retrospective, consecutive, single-center case series the medical files of eyes which have received DMEK between 2009 and 2012 for the treatment of endothelial dysfunction was evaluated regarding follow-up time and clinical outcomes. Annual examinations of best-corrected visual acuity (BCVA), endothelial cell density (ECD), central corneal thickness (CCT) of 66 eyes which fulfilled the criterion of a minimum of 8 years follow-up were analyzed. Results BCVA improved from 0.55 +/- 0.37 logMAR (n = 54) to 0.15 +/- 0.11 (n = 47) in eyes without ocular comorbidities one year after DMEK (p < 0.001), and remained stable up to 10 years after DMEK. Mean ECD decreased to 744 +/- 207 cells/mm(2) (n = 39) after 9 years, and to 729 +/- 167 cells/mm(2) (n = 21) after 10 years, respectively. CCT decreased from 650 +/- 67 mu m before DMEK to 525 +/- 40 mu m (n = 56) after 1 year, increasing slowly to 563 +/- 40 mu m (n = 39) after 9 years, and to 570 +/- 42 mu m (n = 21) after 10 years, respectively. Graft failure occurred in 4 of 66 eyes after year 8. These 4 eyes required repeat DMEK after 101-127 months. Conclusion This study shows the long-term outcomes in a small subset of DMEK grafts. Visual acuity remained stable in spite of slowly increasing corneal thickness and diminishing endothelial cell density during the 10-year period after DMEK.},
author = {Weller, Julia and Kruse, Friedrich and Tourtas, Theofilos},
doi = {10.1007/s10792-021-02176-3},
faupublication = {yes},
journal = {International Ophthalmology},
month = {Jan},
note = {CRIS-Team WoS Importer:2022-01-14},
peerreviewed = {Yes},
title = {{Descemet} membrane endothelial keratoplasty: analysis of clinical outcomes of patients with 8-10 years follow-up},
year = {2022}
}
@article{faucris.122831984,
abstract = {To investigate the outcome of Descemet membrane endothelial keratoplasty (DMEK) in patients with graft failure after Descemet stripping automated endothelial keratoplasty (DSAEK).Retrospective cohort study.setting: Institutional.Fifteen eyes of 15 patients that underwent DMEK for graft failure with corneal decompensation following DSAEK were analyzed; 15 eyes with primary DMEK for Fuchs corneal dystrophy were included as control group.Best-corrected visual acuity (BCVA), endothelial cell density (ECD), central corneal thickness (CCT), and rebubbling rate.DMEK surgery was successful in all cases of both groups. Mean BCVA (logMAR) before DMEK was 1.27 ± 0.34 in the DMEK after DSAEK group and 1.0 ± 0.40 in the Primary DMEK group. After DMEK, mean BCVA increased significantly to 0.23 ± 0.21 (P = .012, DMEK after DSAEK group) and 0.29 ± 0.23 (P = .042, Primary DMEK group) after 3 months. There were no significant differences in mean BCVA between both groups at each visit. The rebubbling rate was 13% in the DMEK after DSAEK group and 40% in the Primary DMEK group (P = .1). Mean CCT decreased significantly in both groups 1 month after DMEK (P < .05). Mean ECD and change of ECD did not differ significantly between both groups at each visit (P > .05).The results after DMEK as a procedure to treat graft failure after DSAEK were as good as in patients that underwent DMEK as primary intervention to treat advanced Fuchs dystrophy. This indicates that the optical quality can be reestablished by DMEK in patients with failed DSAEK.},
author = {Weller, Julia M. and Tourtas, Theofilos and Kruse, Friedrich and Schlötzer-Schrehardt, Ursula and Fuchsluger, Thomas and Bachmann, Bjoern O.},
doi = {10.1016/j.ajo.2015.03.010},
faupublication = {yes},
journal = {American Journal of Ophthalmology},
note = {EVALuna2:21229},
pages = {1050-1057.e2},
peerreviewed = {Yes},
title = {{Descemet} membrane endothelial keratoplasty as treatment for graft failure after descemet stripping automated endothelial keratoplasty},
volume = {159},
year = {2015}
}
@article{faucris.106704444,
abstract = {To evaluate the functional and morphologic outcome of Descemet membrane endothelial keratoplasty (DMEK) combined with phacoemulsification and intraocular lens implantation in patients suffering from endothelial dysfunction and cataract.Retrospective, single-center, consecutive case series.Triple-DMEK (DMEK with simultaneous cataract surgery) was performed in 61 consecutive eyes of 56 patients using corneal donor tissue pre-stored in either short-term culture (Optisol-GS) at 4 C or organ culture (Dulbecco's modified Eagle's medium, CorneaMax medium) at 34 C. Main outcome measures included the number of air injections necessary for graft attachment as well as best-corrected visual acuity (BCVA [logMAR]), central corneal thickness (CCT), endothelial cell density (ECD), refractive spherical equivalent, refractive cylinder, and topographic cylinder at 1, 3, and 6 months postoperatively.BCVA increased from 0.6 ± 0.23 logMAR preoperatively (n = 54) to 0.19 ± 0.22 logMAR at 6 months (n = 27) after surgery (P <= .05). ECD of donor corneas decreased from 2573 ± 235 cells/mm(2) (n = 61) to 1550 ± 326 cells/mm(2) (n = 29) after 6 months (P <= .05). CCT decreased from 651 ± 69 ?m (n = 54) preoperatively to 521 ± 65 ?m (n = 27) after 6 months (P <= .05). Refractive spherical equivalent was -0.3 ± 2.8 D (n = 27) preoperatively and 0.9 ± 1.5 D 6 months (n = 27) after surgery. A total of 54.5% of eyes were within 1 D of emmetropia (n = 12) and 77.3% were within 2 D of emmetropia (n = 17) 6 months (n = 22) after surgery. Refractive cylinder was -0.9 ± 1.0 D preoperatively (n = 49) and -1.5 ± 1.0 D 6 months (n = 23) after surgery. The change in refractive cylinder within the first month was statistically significant (P <= .05; Wilcoxon test). Topographic cylinder was 2.1 ± 1.7 D preoperatively (n = 58) and 1.7 ± 1.1 D 6 months (n = 28) after surgery. Between 3 and 6 months a significant change in topographic cylinder towards lower values was measured (P <<= 0.05; Wilcoxon test). Optimized spherical results were achieved by selecting intraocular lenses based on a hyperopic shift of -0.75 D.DMEK combined with cataract surgery (triple procedure) can routinely be performed in cases of endothelial dystrophy and cataract. The addition of cataract surgery to DMEK had no adverse effect on endothelial function or graft adhesion and did not increase the likelihood of postoperative complications.},
author = {Laaser, Kathrin and Bachmann, Bjoern O. and Horn, Folkert and Cursiefen, Claus and Kruse, Friedrich},
doi = {10.1016/j.ajo.2012.01.020},
faupublication = {yes},
journal = {American Journal of Ophthalmology},
note = {EVALuna2:21089},
pages = {47-55.e2},
peerreviewed = {Yes},
title = {{Descemet} membrane endothelial keratoplasty combined with phacoemulsification and intraocular lens implantation: advanced triple procedure},
volume = {154},
year = {2012}
}
@article{faucris.106569364,
abstract = {To evaluate visual outcome and endothelial cell survival after Descemet membrane endothelial keratoplasty (DMEK) in comparison with Descemet stripping automated endothelial keratoplasty (DSAEK).Single-center, retrospective, consecutive case series.Thirty-eight eyes of 38 consecutive patients undergoing DMEK, who completed a 6-month follow-up, were compared with 35 eyes of 35 consecutive patients undergoing DSAEK for Fuchs endothelial dystrophy or pseudophakic bullous keratopathy. Main outcome measures included best-corrected visual acuity (in logarithm of the minimal angle of resolution [logMAR] units) and endothelial cell density within a 6-month follow-up.Best-corrected visual acuity increased from 0.70 ± 0.48 logMAR and 0.75 ± 0.32 logMAR before surgery to 0.21 ± 0.14 logMAR and 0.48 ± 0.19 logMAR 3 months after DMEK and DSAEK (P < .001), respectively, and to 0.17 ± 0.12 logMAR and 0.36 ± 0.15 logMAR 6 months after DMEK and DSAEK (P < .001), respectively. Endothelial cell density decreased from 2575 ± 260 cells/mm(2) and 2502 ± 220 cells/mm(2) before surgery to 1498 ± 244 cells/mm(2) and 1778 ± 420 cells/mm(2) 3 months after DMEK and DSAEK (P < .001), respectively, and to 1520 ± 299 cells/mm(2) and 1532 ± 495 cells/mm(2) 6 months after DMEK and DSAEK (P = .483), respectively. Central corneal thickness decreased from 652 ± 92 ?m before surgery to 517 ± 45 ?m 6 months after DMEK, and from 698 ± 137 ?m before surgery to 618 ± 66 ?m 6 months after DSAEK.DMEK provided faster and more complete visual rehabilitation when compared with DSAEK. However, there were no significant differences concerning endothelial cell survival within a 6-month follow-up.},
author = {Tourtas, Theofilos and Laaser, Kathrin and Bachmann, Bjoern O. and Cursiefen, Claus and Kruse, Friedrich},
doi = {10.1016/j.ajo.2011.12.012},
faupublication = {yes},
journal = {American Journal of Ophthalmology},
note = {EVALuna2:21063},
pages = {1082-90.e2},
peerreviewed = {Yes},
title = {{Descemet} membrane endothelial keratoplasty versus descemet stripping automated endothelial keratoplasty},
volume = {153},
year = {2012}
}
@article{faucris.106662204,
abstract = {To study the potential use of human donor anterior lens capsule as a Descemet's membrane substrate.Anterior lens capsules were recovered from the lenses of 30 cornea donors. Human corneal endothelial cells were recovered from the remaining corneal sclera rims of 15 donor corneas used for penetrating keratoplasty. Samples were sorted into three groups. Group 1 consisted of 10 samples in which the endothelial cells were allowed to grow on anterior lens capsules. In Group 2 human corneal endothelial cells grew on a collagen membrane and in Group 3 on polystyrene culture plates. Cell density, morphology and adherence of the cell-capsule complex were evaluated at 1, 4, 7 and 14days with a phase-contrast microscope, a scanning electron microscope and by histology. Cell viability was quantified by a microscopic live-dead assay. Expression of zonula occludens-1, Na(+) /K(+) -adenosine triphosphatase, tissue transglutaminase and vimentin were investigated by immunohistochemistry.A mean diameter of 10.05±0.13mm of anterior capsule was obtained as a substrate for cell culture. Endothelial cell density of Group 1 was measured at 2455.4±283.8cells/mm(2) , which was also comparable with the cell density of the control group. Cell viability was 95% or superior in all groups and multiple cellular interconnections developed between growing cells. Immunohistochemical analysis demonstrated strongly positive staining for all investigated proteins. Electron microscopy confirmed the adherence and monolayer growth of the endothelial cells.Human donor anterior lens capsule might therefore be a potential scaffold for the ex vivo expansion of human corneal endothelial cells.},
author = {Kopsachilis, Nikolaos and Tsinopoulos, Ioannis and Tourtas, Theofilos and Kruse, Friedrich and Luessen, Ulrich Welge},
doi = {10.1111/j.1442-9071.2011.02678.x},
faupublication = {yes},
journal = {Clinical and Experimental Ophthalmology},
note = {EVALuna2:21107},
pages = {187-94},
peerreviewed = {Yes},
title = {{Descemet}'s membrane substrate from human donor lens anterior capsule},
volume = {40},
year = {2012}
}
@inproceedings{faucris.228454778,
address = {ROCKVILLE},
author = {Menzel-Severing, Johannes and Geerling, Gerd and Kruse, Friedrich and Walter, Peter and Salla, Sabine},
booktitle = {INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE},
date = {2019-04-28/2019-05-02},
faupublication = {yes},
note = {CRIS-Team WoS Importer:2019-10-29},
peerreviewed = {unknown},
publisher = {ASSOC RESEARCH VISION OPHTHALMOLOGY INC},
title = {{Deturgescence} using dextran negatively affects corneal endothelial cell viability of pre-stripped grafts for {Descemet}'s {Membrane} {Endothelial} {Keratoplasty}},
venue = {Vancouver},
year = {2019}
}
@article{faucris.106654284,
abstract = {To investigate the effect of culture conditions of donor tissue on functional outcome after Descemet membrane endothelial keratoplasty.Retrospective, single-center, consecutive case series.Descemet membrane endothelial keratoplasty was performed routinely in 82 eyes of 82 consecutive patients using corneal donor tissue prestored in either short-term culture (Optisol-GS; Bausch & Lomb) at 4 C (group A; n = 37) or organ culture (Dulbecco Modified Eagle Medium [Biochrom]; CorneaMax Medium [Eurobio]) at 34 C (group B; n = 45) in a randomized fashion. Main outcome measures included the number of air injections necessary for graft attachment as well as best-corrected visual acuity (in logarithm of the minimal angle of resolution [logMAR] units), central corneal thickness, and endothelial cell density at 1, 3, and 6 months after surgery.Best-corrected visual acuity increased from 0.69 ± 0.53 logMAR and 0.67 ± 0.31 logMAR before surgery to 0.33 ± 0.21 logMAR and 0.28 ± 0.18 logMAR after 1 month (P < .05), to 0.24 ± 0.16 logMAR and 0.18 ± 0.16 logMAR after 3 months (P < .05), and to 0.18 ± 0.12 logMAR and 0.15 ± 0.10 logMAR after 6 months (n.s.) in groups A and B, respectively. Endothelial cell density decreased from 2647 ± 236 cells/mm(2) and 2515 ± 249 cells/mm(2) before surgery to 1499 ± 277 cells/mm(2) and 1526 ± 205 cells/mm(2) after 1 month (P < .05), to 1441 ± 213 cells/mm(2) and 1443 ± 316 cells/mm(2) after 3 months (n.s.), and to 1587 ± 366 cells/mm(2) and 1457 ± 285 cells/mm(2) after 6 months (n.s.) in groups A and B, respectively. Central corneal thickness declined from 664 ± 89 and 662 ± 107 ?m before surgery to 529 ± 92 ?m and 517 ± 62 ?m after 1 month (P < .05), to 511 ± 46 ?m and 510 ± 46 ?m after 3 months (P < .05), and to 529 ± 68 ?m and 507 ± 50 ?m after 6 months (n.s.) in groups A and B, respectively. Best-corrected visual acuity, endothelial cell density, and central corneal thickness values showed no significant differences between both groups at any time point after surgery. However, a significantly higher total number of air injections was necessary in group A (n = 34) compared with group B (n = 26) to obtain graft attachment (P < .05).These findings suggest that donor tissue culture conditions have no significant effect on functional outcome, but may influence graft adhesion and rebubbling rate after Descemet membrane endothelial keratoplasty surgery.},
author = {Laaser, Kathrin and Bachmann, Bjoern O. and Horn, Folkert and Schlötzer-Schrehardt, Ursula and Cursiefen, Claus and Kruse, Friedrich},
doi = {10.1016/j.ajo.2010.11.027},
faupublication = {yes},
journal = {American Journal of Ophthalmology},
note = {EVALuna2:21002},
pages = {1007-1018.e2},
peerreviewed = {Yes},
title = {{Donor} tissue culture conditions and outcome after descemet membrane endothelial keratoplasty},
volume = {151},
year = {2011}
}
@inproceedings{faucris.228453286,
address = {ROCKVILLE},
author = {Nakagawa, Risako and Okumura, Naoki and Onishi, Takako and Oshima, Takeshi and Ueda, Emi and Watanabe, Kyoko and Tourtas, Theofilos and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich and Koizumi, Noriko},
booktitle = {INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE},
date = {2019-04-28/2019-05-02},
faupublication = {yes},
note = {CRIS-Team WoS Importer:2019-10-29},
peerreviewed = {unknown},
publisher = {ASSOC RESEARCH VISION OPHTHALMOLOGY INC},
title = {{Drug} discovery for the treatment of {Fuchs} corneal endothelial dystrophy by cell-based drug screening system},
venue = {Vancouver, CANADA},
year = {2019}
}
@article{faucris.260269859,
abstract = {Purpose: Abnormalities in the limbal niche microenvironment have been suggested to be causally involved in aniridia-associated keratopathy (AAK), but histological analyses on the limbal structure and composition in AAK are lacking. Here, we investigated morphologic and molecular alterations of the limbal epithelial stem cell niche in human congenital aniridia. Methods: The blind, buphthalmic and painful left eye of a 16-year old girl with congenital aniridia and juvenile glaucoma had to be enucleated because of uncontrolled intraocular pressure. The diagnosis of AAK was based on classical clinical features and partial limbal stem cell deficiency in the superior half. Genetic analysis identified a large heterozygous PAX6 gene deletion encompassing exons 11–15 as well as exon 9 of the neighboring ELP4 gene. Three limbal biopsies were taken from the superior, nasal and temporal regions to isolate and cultivate limbal epithelial progenitor cells and subject them to mRNA expression analyses. The globe was vertically bisected and processed for light and transmission electron microscopy and immunohistochemistry. Results: Comparative analysis of the superior and inferior limbal zones showed a gradual degradation of palisade structures associated with the transition from a hyperplastic to an attenuated corneal epithelium, inflammatory cell infiltrations and basement membrane irregularities. The clinically unaffected inferior part revealed no distinct stem cell clusters in the preserved palisade region, but a uniform population of hyperproliferative, undifferentiated progenitor cells in the basal/suprabasal layers of limbal and corneal epithelia, which gave rise to maldifferentiated epithelial cells exhibiting a conjunctival/epidermal phenotype and nuclear-to-cytoplasmic translocation of Pax6. The structure of the limbal niche was fundamentally perturbed, showing marked alterations in extracellular matrix composition, dislocation of atypical melanocytes lacking melanosomes and melanin, aberrant Wnt/β-catenin and retinoic acid signaling, and massive immune cell infiltration. Conclusions: Considering the limitations of a single Case study, the findings suggest that ocular surface alterations in AAK are caused by a primary dysfunction and gradual breakdown of the limbal stem cell niche through Pax6-related effects on both melanogenesis and epithelial differentiation.},
author = {Schlötzer-Schrehardt, Ursula and Latta, Lorenz and Gießl, Andreas and Zenkel, Matthias and Fries, Fabian N. and Käsmann-Kellner, Barbara and Kruse, Friedrich and Seitz, Berthold},
doi = {10.1016/j.jtos.2021.06.002},
faupublication = {yes},
journal = {Ocular Surface},
keywords = {Aniridia-associated keratopathy; Extracellular matrix; Immunohistochemistry; Limbal stem cell niche; Melanocytes; Pax6},
note = {CRIS-Team Scopus Importer:2021-06-18},
pages = {160-173},
peerreviewed = {Yes},
title = {{Dysfunction} of the limbal epithelial stem cell niche in aniridia-associated keratopathy: {Limbal} niche in aniridia-associated keratopathy},
volume = {21},
year = {2021}
}
@article{faucris.277082410,
abstract = {Pseudoexfoliation (PEX) syndrome, a stress-induced fibrotic matrix process, is the most common recognizable cause of open-angle glaucoma worldwide. The recent identification of PEX-associated gene variants uncovered the vitamin A metabolic pathway as a factor influencing the risk of disease. In this study, we analyzed the role of the retinoic acid (RA) signaling pathway in the PEX-associated matrix metabolism and evaluated its targeting as a potential candidate for an anti-fibrotic intervention. We provided evidence that decreased expression levels of RA pathway components and diminished RA signaling activity occur in an antagonistic crosstalk with TGF-beta 1/Smad signaling in ocular tissues and cells from PEX patients when compared with age-matched controls. Genetic and pharmacologic modes of RA pathway inhibition induced the expression and production of PEX-associated matrix components by disease-relevant cell culture models in vitro. Conversely, RA signaling pathway activation by natural and synthetic retinoids was able to suppress PEX-associated matrix production and formation of microfibrillar networks via antagonization of Smad-dependent TGF-beta 1 signaling. The findings indicate that deficient RA signaling in conjunction with hyperactivated TGF-beta 1 /Smad signaling is a driver of PEX-associated fibrosis, and that restoration of RA signaling may be a promising strategy for anti-fibrotic intervention in patients with PEX syndrome and glaucoma.},
author = {Zenkel, Matthias and Hoja, Ursula and Gießl, Andreas and Berner, Daniel and Hohberger, Bettina and Weller, Julia and König, Loretta and Huebner, Lisa and Ostermann, Thomas A. and Gusek-Schneider, Gabriele and Kruse, Friedrich and Pasutto, Francesca and Schlötzer-Schrehardt, Ursula},
doi = {10.3390/ijms23115977},
faupublication = {yes},
journal = {International Journal of Molecular Sciences},
keywords = {pseudoexfoliation syndrome; pseudoexfoliation glaucoma; retinol; retinoic acid; extracellular matrix; fibrosis; TGF-β1},
note = {CRIS-Team WoS Importer:2022-06-24},
peerreviewed = {Yes},
title = {{Dysregulated} {Retinoic} {Acid} {Signaling} in the {Pathogenesis} of {Pseudoexfoliation} {Syndrome}},
volume = {23},
year = {2022}
}
@inproceedings{faucris.242414916,
address = {ROCKVILLE},
author = {Gießl, Andreas and Polisetti, Naresh and Li, Shen and Kruse, Friedrich and Schlötzer-Schrehardt, Ursula},
booktitle = {INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE},
date = {2020-05-01/2020-05-07},
faupublication = {yes},
note = {CRIS-Team WoS Importer:2020-09-11},
peerreviewed = {unknown},
publisher = {ASSOC RESEARCH VISION OPHTHALMOLOGY INC},
title = {{Effective} isolation and expansion of human limbal niche cells},
venue = {ONLINE},
year = {2020}
}
@inproceedings{faucris.242417405,
address = {ROCKVILLE},
author = {Matsuo, Hinata and Okumura, Naoki and Komori, Yuya and Tokunaga, Ayumi and Nakayama, Genta and Nakahara, Makiko and Blake, Derek J. and Tourtas, Theofilos and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich and Koizumi, Noriko},
booktitle = {INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE},
date = {2020-05-01/2020-05-07},
faupublication = {yes},
note = {CRIS-Team WoS Importer:2020-09-11},
peerreviewed = {unknown},
publisher = {ASSOC RESEARCH VISION OPHTHALMOLOGY INC},
title = {{Effect} of {CTG} repeat expansion in {TCF4} on the pathophysiology of {Fuchs} endothelial corneal dystrophy},
venue = {ONLINE},
year = {2020}
}
@article{faucris.213493922,
abstract = {PURPOSE. CTG trinucleotide repeat (TNR) expansion is frequently found in transcription factor 4 (TCF4) in Fuchs' endothelial corneal dystrophy (FECD), though the effect of TNR expansion on FECD pathophysiology remains unclear. The purpose of this study was to evaluate the effect of TNR expansion on TCF4 expression in corneal endothelium of patients with FECD.},
author = {Okumura, Naoki and Hayashi, Ryosuke and Nakano, Masakazu and Yoshii, Kengo and Tashiro, Kei and Sato, Takahiko and Blake, Derek J. and Aleff, Ross and Butz, Malinda and Highsmith, Edward W. and Wieben, Eric D. and Fautsch, Michael P. and Baratz, Keith H. and Komori, Yuya and Nakahara, Makiko and Tourtas, Theofilos and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich and Koizumi, Noriko},
doi = {10.1167/iovs.18-25760},
faupublication = {yes},
journal = {Investigative Ophthalmology & Visual Science},
note = {CRIS-Team WoS Importer:2019-03-15},
pages = {779-786},
peerreviewed = {Yes},
title = {{Effect} of {Trinucleotide} {Repeat} {Expansion} on the {Expression} of {TCF4} {mRNA} in {Fuchs}' {Endothelial} {Corneal} {Dystrophy}},
volume = {60},
year = {2019}
}
@article{faucris.106656044,
abstract = {Purpose: To determine whether indomethacin 0.1% eye drops are at least as effective as ketorolac 0.5% eye drops in treating ocular inflammation following cataract surgery. Methods: Prospective, multicenter, investigator-masked, parallel-group, randomized, active-controlled clinical trial. Cataract patients were randomized in a 1:1 ratio to receive indomethacin or ketorolac administered QID for 3 weeks beginning 1 day before surgery. The primary end-point was aqueous flare measured by laser flare meter at postoperative Days 1 and 7. Secondary end-points included retinal thickness, slit lamp and funduscopic examinations and postsurgical pain ratings. Safety and tolerability were also assessed. Results: A total of 86 patients were included in the per protocol population (n = 43 per treatment group). Indomethacin was found non-inferior to ketorolac for comparison of aqueous flare at postoperative Days 1 and 7 (Day 1: 95% CI: -2.37, 5.50; non-inferiority upper margin, 15 ph/ms and Day 7: 95% CI: -7.83, -0.94; non-inferiority upper margin, 8 ph/ms) and statistically better than ketorolac at Day 7 (p = 0.013). There were no significant between-group differences in aqueous flare and change from baseline in retinal thickness at postoperative Days 30 and 90. Indomethacin showed a higher subjective tolerance rating than ketorolac at postoperative Days 7 and 30 (p <= 0.044). Conclusion: Indomethacin 0.1% was at least as effective as ketorolac 0.5% at Day 1 and more effective than ketorolac 0.5% at Day 7 in treating ocular inflammation after uncomplicated cataract surgery. Indomethacin was better tolerated than ketorolac. There were no clinically meaningful safety concerns with either treatment.},
author = {Weber, Michel and Kodjikian, Laurent and Kruse, Friedrich and Zagorski, Zbigniew and Allaire, Catherine M.},
doi = {10.1111/j.1755-3768.2012.02520.x},
faupublication = {yes},
journal = {Acta Ophthalmologica},
note = {EVALuna2:21108},
pages = {e15-21},
peerreviewed = {Yes},
title = {{Efficacy} and safety of indomethacin 0.1% eye drops compared with ketorolac 0.5% eye drops in the management of ocular inflammation after cataract surgery},
volume = {91},
year = {2013}
}
@article{faucris.110922724,
abstract = {To develop a safe and efficient method for targeted, anti-apoptotic gene therapy of corneal endothelial cells (CECs).Magnetofection (MF), a combination of lipofection with magnetic nanoparticles (MNPs; PEI-Mag2, SO-Mag5, PalD1-Mag1), was tested in human CECs and in explanted human corneas. Effects on cell viability and function were investigated. Immunocompatibility was assessed in human peripheral blood mononuclear cells.Silica iron-oxide MNPs (SO-Mag5) combined with X-tremeGENE-HP achieved high transfection efficiency in human CECs and explanted human corneas, without altering cell viability or function. Magnetofection caused no immunomodulatory effects in human peripheral blood mononuclear cells. Magnetofection with anti-apoptotic P35 gene effectively blocked apoptosis in CECs.Magnetofection is a promising tool for gene therapy of corneal endothelial cells with potential for targeted on-site delivery.},
author = {Czugala, Marta and Mykhaylyk, Olga and Boehler, Philip and Onderka, Jasmine and Stork, Boejrn and Wesselborg, Sebastian and Kruse, Friedrich and Plank, Christian and Singer, Bernhard B. and Fuchsluger, Thomas},
doi = {10.2217/nnm-2016-0144},
faupublication = {yes},
journal = {Nanomedicine},
note = {EVALuna2:21292},
pages = {1787-800},
peerreviewed = {Yes},
title = {{Efficient} and safe gene delivery to human corneal endothelium using magnetic nanoparticles},
volume = {11},
year = {2016}
}
@article{faucris.109586224,
abstract = {Increased expression of glial fibrillary acidic protein (GFAP) is a characteristic of gliotic activation (Müller cells and astrocytes) in the retina. This study assessed vitreous body GFAP levels in various forms of retinal pathology.This prospective study included 82 patients who underwent vitrectomy (46 retinal detachments (RDs), 13 macular hole (MHs), 15 epiretinal glioses (EGs), 8 organ donors). An established enzyme-linked immunosorbent assay (ELISA, SMI26) was used for quantification of GFAP.The highest concentration of vitreous body GFAP in organ donors was 20 pg/mL and it was used as the cutoff. A significant proportion of patients suffering from RD (65 %) to EG (53 %) had vitreous body GFAP levels above this cutoff when compared to organ donors (0 %, p < 0.0001, p = 0.0194, respectively, Fisher's exact test) and MH (8 %, p < 0.0001, p = 0.0157, respectively). In RD and EG, vitreous body GFAP levels were correlated with axial length (R = 0.69, R = 0.52, p < 0.05 for both).The data suggest that human vitreous body GFAP is a protein biomarker for glial activation in response to retinal pathologies. Vitreous body GFAP levels may be of interest as a surrogate outcome for experimental treatment strategies in translational studies.},
author = {Jünemann, Anselm and Rejdak, Robert and Huchzermeyer, Cord and Maciejewski, Ryszard and Grieb, Pawel and Kruse, Friedrich and Zrenner, Eberhart and Rejdak, Konrad and Petzold, Axel},
doi = {10.1007/s00417-015-3127-7},
faupublication = {yes},
journal = {Graefes Archive For Clinical and Experimental Ophthalmology},
note = {EVALuna2:21235},
pages = {2181-6},
peerreviewed = {Yes},
title = {{Elevated} vitreous body glial fibrillary acidic protein in retinal diseases},
volume = {253},
year = {2015}
}
@inproceedings{faucris.208857703,
author = {Oshima, Takeshi and Okumura, Naoki and Onishi, Takako and Ueda, Emi and Watanabe, Kyoko and Tourtas, Theofilos and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich and Koizumi, Noriko},
faupublication = {yes},
note = {EVALuna2:34744},
peerreviewed = {Yes},
title = {{Establishment} of a drug screening system for {Fuchs} endothelial corneal dystrophy},
volume = {59},
year = {2018}
}
@article{faucris.122160764,
abstract = {We report our findings from a preclinical safety study designed to assess potential side effects of corneal ultraviolet femtosecond laser treatment on lens and retina.Refractive lenticules (-5 dpt) with a diameter of 6 mm were created in the right cornea of eight Dutch Belted rabbits. Radiant exposure was 0.5 J/cm² in two animals and 18 J/cm² in six animals. The presence of lens opacities was assessed prior to and up to six months following laser application using Scheimpflug images (Pentacam, Oculus) and backscatter analysis (Opacity Lensmeter 702, Interzeag). Ganzfeld flash and flicker electroretinogram (ERG) recordings were obtained from both eyes prior to and up to six weeks following laser application. At the study endpoint, retinas were examined by light microscopy.Independent of energy dose applied, no cataract formation could be observed clinically or with either of the two objective methods used. No changes in ERG recordings over time and no difference between treated and untreated eye were detected. Histologically, retinal morphology was preserved and retinal pigment epithelium as well as photoreceptor inner and outer segments appeared undamaged. Quantitative digital image analysis did not reveal cell loss in inner or outer nuclear layers.Our analysis confirms theoretical considerations suggesting that ultraviolet femtosecond laser treatment of the cornea is safe for intraocular tissues. Transmitted light including stray light induces no photochemical effects in lens or retina at energy levels much higher than required for the clinical purpose. These conclusions cannot be applied to eyes with pre-existing retinal damage, as these may be more vulnerable to light.},
author = {Menzel-Severing, Johannes and Petsch, Corinna and Tourtas, Theofilos and Polisetti, Naresh and Klenke, Joerg and Skerl, Katrin and Wuellner, Christian and Donitzky, Christof and Kruse, Friedrich and Kremers, Jan and Hammer, Christian},
doi = {10.1371/journal.pone.0137638},
faupublication = {yes},
journal = {PLoS ONE},
note = {EVALuna2:4434},
pages = {e0137638},
peerreviewed = {Yes},
title = {{Evaluation} of a 345 nm {Femtosecond} {Laser} for {Corneal} {Surgery} with {Respect} to {Intraocular} {Radiation} {Hazard}},
volume = {10},
year = {2015}
}
@article{faucris.109144244,
abstract = {The aim of this study was to compare central corneal thickness (CCT) between corneas of normal healthy eyes (cNHE), corneas of eyes that had undergone cataract surgery by clear corneal phacoemulsification with implantation of an intracapsular intraocular lens (cIOL), corneal grafts after penetrating keratoplasty (gPK) and corneas of long-term soft contact lens wearers (cCL).The study design was a consecutive cross-sectional trial. CCT was measured using rotating Scheimpflug camera (Pentacam, software version 1.16r04) in 80 cNHE, 79 cIOL, 46 gPK and 78 cCL. Analysis of variance (one-way ANOVA) was performed to compare differences of mean values between these four groups. Pearson's or Spearman's correlation coefficient (r) was determined between CCT value and age, follow up time after penetrating keratoplasty (timePK) or contact lens wearing time (timeCL).Means of CCT measurements were comparable between cNHE (mean CCT±standard deviation, 554±36?m), cIOL (551±40?m) and gPK (534±52?m) as determined by one-way ANOVA. Mean CCT values in cCL (537±37?m) were statistically significantly lower in comparison to cNHE (p=0.026, 95% CI=1.43-31.44). There was no linear correlation between age and CCT values of cNHE and cIOL (p=0.841, r=-0.031 and p=0.931, r=0.011, respectively). No linear relationship was determined between CCT values of cCL and timeCL (p=0.315, r=-0.125). CCT values of gPK did not correlate with timePK (p=0.738, r=0.054).The data reported here indicate that in the same statistical model among CCT values of cNHE, cIOL and gPK only long-term soft contact lenses (CL) wearer have significantly lower CCT measurements.},
author = {Sel, Saadettin and Trau, Stefanie and Knak, Matthias and Kalinski, Thomas and Kaiser, Delia and Kruse, Friedrich and Huchzermeyer, Cord and Duncker, Gernot I. W. and Paulsen, Friedrich and Auffarth, Gerd U. and Nass, Norbert},
doi = {10.1016/j.clae.2013.03.002},
faupublication = {yes},
journal = {Contact Lens and Anterior Eye},
note = {EVALuna2:4398},
pages = {238-42},
peerreviewed = {Yes},
title = {{Evaluation} of central corneal thickness after cataract surgery, penetrating keratoplasty and long-term soft contact lens wear},
volume = {36},
year = {2013}
}
@article{faucris.106665504,
abstract = {Pseudoexfoliation (PEX) syndrome is a systemic disorder of the elastic fiber system that can lead to PEX glaucoma. Elastotic alterations in the lamina cribrosa (LC) of PEX eyes suggested biomechanical implications predisposing to pressure-induced optic nerve damage. In this pilot study, the stiffness of LC and peripapillary sclera (ppSC) in eyes with and without PEX syndrome were analyzed by atomic force microscopy (AFM) nanoindentation.Unfixed cryosections (5-?m thick) were prepared from the optic nerve heads (ONH) of three donor eyes with PEX syndrome and three age-matched control eyes. AFM force mapping was performed in selected regions of the central, midperipheral, and peripheral LC and the ppSC using a spherical cantilever tip. To determine the local Young's modulus of elasticity (YME) as a measure of tissue stiffness, force curves were acquired and analyzed using the spherical Hertz model.For the LC, the median YME values calculated from single stiffness maps averaged 17.2 (±2.7) kPa in normal eyes and 10.1 (±1.4) kPa in PEX eyes, indicating a significant PEX-related decrease in stiffness by over 40% (P < 0.01). The corresponding YME values for the ppSC, which revealed a 9-fold higher tissue stiffness than in the LC, averaged 158.3 (±59.8) kPa for control and 85.8 (±16.9) kPa for PEX samples.AFM was proven suitable for determining the stiffness of ONH tissues, encouraging further large-scale analyses. The marked decrease in stiffness, implying an increased deformability of the ONH in PEX eyes, may reflect an inherent tissue weakness rendering these eyes more vulnerable to glaucomatous damage.},
author = {Braunsmann, Christoph and Hammer, Christian M. and Rheinlaender, Johannes and Kruse, Friedrich and Schäffer, Tilman and Schlötzer-Schrehardt, Ursula},
doi = {10.1167/iovs.11-8409},
faupublication = {yes},
journal = {Investigative Ophthalmology & Visual Science},
note = {EVALuna2:21095},
pages = {2960-7},
peerreviewed = {Yes},
title = {{Evaluation} of lamina cribrosa and peripapillary sclera stiffness in pseudoexfoliation and normal eyes by atomic force microscopy},
volume = {53},
year = {2012}
}
@article{faucris.110059884,
abstract = {Glaucoma is a genetically heterogeneous disorder and is the second cause of blindness worldwide owing to the progressive degeneration of retinal ganglion neurons. Very few genes causing glaucoma were identified to this date. In this study, we screened 10 candidate genes of glaucoma between the D14S261 and D14S121 markers of chromosome 14q11, a critical region previously linked to primary open-angle glaucoma (POAG). Mutation analyses of two large cohorts of patients with POAG, normal tension glaucoma (NTG) and juvenile open-angle glaucoma (JOAG), and control subjects, found only association of non-synonymous heterozygous variants of the retinitis pigmentosa GTPase regulator-interacting protein 1 (RPGRIP1) with POAG, NTG and JOAG. The 20 non-synonymous variants identified in RPGRIP1 were all distinct from variants causing photoreceptor dystrophies and were found throughout all but one domain (RPGR-interacting domain) of RPGRIP1. Among them, 14 missense variants clustered within or around the C2 domains of RPGRIP1. Yeast two-hybrid analyses of a subset of the missense mutations within the C2 domains of RPGRIP1 shows that five of them (p.R598Q, p.A635G, p.T806I, p.A837G and p.I838V) decrease the association of the C2 domains with nephrocystin-4 (NPHPH). When considering only these five confirmed C2-domain mutations, the association remains statistically significant (P=0.001). Altogether, the data support that heterozygous non-synonymous variants of RPGRIP1 may cause or increase the susceptibility to various forms of glaucoma and that among other factors, physical impairment of the interaction of RPGRIP1with different proteins may contribute to the pathogenesis of forms of glaucoma.},
author = {Fernández Martinez, Lorena and Letteboer, Stef and Mardin, Christian Y. and Weisschuh, Nicole and Gramer, Eugen and Weber, Bernhard H. F. and Rautenstrauß, Bernd and Ferreira, Paulo A. and Kruse, Friedrich and Reis, André and Roepman, Ronald and Pasutto, Francesca},
doi = {10.1038/ejhg.2010.217},
faupublication = {yes},
journal = {European journal of human genetics},
note = {EVALuna2:9085},
pages = {445-51},
peerreviewed = {Yes},
title = {{Evidence} for {RPGRIP1} gene as risk factor for primary open angle glaucoma},
volume = {19},
year = {2011}
}
@article{faucris.119663104,
abstract = {To investigate the hypothesis that adult corneal endothelial cells can migrate after Descemet membrane endothelial keratoplasty (DMEK).Prospective observational study.Five patients with Fuchs endothelial dystrophy were examined 1 year after uneventful DMEK. These patients had been selected on the basis of slightly decentered grafts and/or large descemetorrhexis showing areas of denuded corneal stroma, which were covered by neither the patients' Descemet membrane (DM) nor the graft. These areas were investigated by in vivo confocal laser scanning microscopy using a specially designed Heidelberg Retina Tomograph II and Rostock cornea module equipped with custom-made software. Source data (frame rate 30 Hz, 384 × 384 pixels, 400 × 400 ?m) were used to create large-scale maps of the scanned area in automatic real-time composite mode. In each case an on-line mapping with maximum size up to 3.2 × 3.2 mm (3072 × 3072 pixels) was performed.Corneal stroma overlying areas devoid of DM was transparent. In vivo confocal laser scanning microscopy of stroma devoid of DM revealed a monolayer of endothelial cells in all patients observed. The morphologic pattern of these cells was similar to that of endothelial cells on DM grafts but different from the morphology of the patients' own endothelium, suggesting migration of donor endothelial cells from DMEK grafts.The results strongly support the hypothesis that adult corneal endothelial cells are able to migrate in the human eye. Furthermore, we provide evidence to support the hypothesis that grafted endothelium migrates onto the host tissue, repopulating the corneal stroma with a regular endothelial phenotype.},
author = {Jacobi, Christina and Zhivov, Andrey and Korbmacher, Judit and Falke, Karen and Guthoff, Rudolf and Schlötzer-Schrehardt, Ursula and Cursiefen, Claus and Kruse, Friedrich},
doi = {10.1016/j.ajo.2011.04.005},
faupublication = {yes},
journal = {American Journal of Ophthalmology},
note = {EVALuna2:21034},
pages = {537-542.e2},
peerreviewed = {Yes},
title = {{Evidence} of endothelial cell migration after descemet membrane endothelial keratoplasty},
volume = {152},
year = {2011}
}
@article{faucris.122636184,
abstract = {To characterize the alterations of extracellular matrix proteins in Descemet's membranes (DM) of patients with late-onset Fuchs' corneal dystrophy (FCD) and to differentiate them from nonspecific alterations in pseudophakic bullous keratopathy (PBK).Human DM-endothelial cell complexes were obtained from patients with late-onset FCD (n = 40), PBK (n = 6), and control eyes (n = 5). Gene expression profiles of endothelial cells were compared using a commercial real-time PCR array and quantitative real-time PCR assays for confirmation of differentially expressed genes. A total of 24 extracellular matrix proteins were also localized in cryosections of corneal specimens from FCD (n = 10), PBK (n = 4), and control eyes (n = 5) by immunohistochemistry.Polymerase chain reaction array analysis revealed a significant upregulation of 27 out of 84 extracellular matrix-related genes including collagens, proteoglycans, glycoproteins, cell adhesion molecules, and matrix metalloproteinases in FCD specimens as compared to normal controls, which could be partly confirmed and quantified by real-time PCR. Comparative analysis of FCD and PBK specimens showed a significant and consistent FCD-specific upregulation of collagen types I, III, and XVI; fibronectin; agrin; clusterin; transforming growth factor beta-induced (TGFBI); and integrin ?4 (3- to 18-fold, P < 0.05). Immunohistochemistry revealed an increased labeling of collagen (types III, VII, XV, XVI), agrin, fibulin-2, TGFBI, versican, and clusterin in the DM of FCD specimens compared to PBK specimens.The findings provide evidence for a specific upregulation, production, and deposition of collagen types III and XVI, agrin, TGFBI, and clusterin in late-onset FCD and thus point to the importance of matrix alterations in the pathophysiology of FCD.},
author = {Weller, Julia M. and Zenkel, Matthias and Schlötzer-Schrehardt, Ursula and Bachmann, Björn and Tourtas, Theofilos and Kruse, Friedrich},
doi = {10.1167/iovs.14-14154},
faupublication = {yes},
journal = {Investigative Ophthalmology & Visual Science},
note = {EVALuna2:21180},
pages = {3700-8},
peerreviewed = {Yes},
title = {{Extracellular} matrix alterations in late-onset {Fuchs}' corneal dystrophy},
volume = {55},
year = {2014}
}
@inproceedings{faucris.228450554,
address = {ROCKVILLE},
author = {Schlötzer-Schrehardt, Ursula and Kruse, Franziska and Kraus, Kerstin and Zenkel, Matthias and Kruse, Friedrich},
booktitle = {INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE},
date = {2019-04-28/2019-05-02},
faupublication = {yes},
note = {CRIS-Team WoS Importer:2019-10-29},
peerreviewed = {unknown},
publisher = {ASSOC RESEARCH VISION OPHTHALMOLOGY INC},
title = {{Extracellular} matrix regulation of limbal epithelial stem cell function},
venue = {Vancouver},
year = {2019}
}
@article{faucris.122486584,
abstract = {Descemet membrane endothelial keratoplasty (DMEK) is becoming the method of choice for treating Fuchs endothelial dystrophy and pseudophakic bullous keratopathy. We investigated whether DMEK can serve as a routine procedure in endothelial decompensation even in complex preoperative situations.Of a total of 1184 DMEK surgeries, 24 consecutive eyes with endothelial decompensation and complex preoperative situations were retrospectively analyzed and divided into 5 groups: group 1: irido-corneo-endothelial syndrome (n = 3), group 2: aphakia, subluxated posterior chamber intraocular lens or anterior chamber intraocular lens (n = 6), group 3: DMEK after trabeculectomy (n = 4), group 4: DMEK with simultaneous intravitreal injection (n = 6), and group 5: DMEK after vitrectomy (n = 5). Main outcome parameters were best-corrected visual acuity, central corneal thickness, endothelial cell density, rebubbling rate, and graft failure rate.Best-corrected visual acuity (logMAR) increased from 0.98 to 0.53 (P = 0.002), 0.53 (P = 0.091), and 0.57 (P = 0.203) after 1, 3, and 6 months, respectively. Central corneal thickness decreased from 731 ± 170 to 546 ± 152 ?m (P = 0.001), 514 ± 66 ?m (P = 0.932), and 554 ± 98 ?m (P = 0.004) after 1, 3, and 6 months, respectively. Donor endothelial cell density decreased from 2478 ± 185 to 1454 ± 193/mm (P < 0.001), 1301 ± 298/mm (P = 0.241), and 1374 ± 261/mm (P = 0.213), after 1, 3, and 6 months, respectively. The rebubbling rate was 46% (11/24). Four patients (17%) had secondary graft failure.Our data provide evidence that DMEK is feasible for the treatment of endothelial decompensation in complex preoperative situations.},
author = {Weller, Julia M. and Tourtas, Theofilos and Kruse, Friedrich},
doi = {10.1097/ICO.0000000000000625},
faupublication = {yes},
journal = {Cornea},
note = {EVALuna2:21233},
pages = {1351-7},
peerreviewed = {Yes},
title = {{Feasibility} and {Outcome} of {Descemet} {Membrane} {Endothelial} {Keratoplasty} in {Complex} {Anterior} {Segment} and {Vitreous} {Disease}},
volume = {34},
year = {2015}
}
@article{faucris.208860045,
abstract = {Corneal transparency is maintained by the corneal endothelium through its pump and barrier function. Severe corneal endothelial damage results in dysregulation of water flow and eventually causes corneal haziness and deterioration of visual function. In 2013, we initiated clinical research of cell-based therapy for treating corneal decompensation. In that study, we removed an 8-mm diameter section of damaged corneal endothelium without removing Descemet's membrane (the basement membrane of the corneal endothelium) and then injected cultured human corneal endothelial cells (CECs) into the anterior chamber. However, Descemet's membrane exhibits clinically abnormal structural features [i.e., multiple collagenous excrescences (guttae) and thickening] in patients with Fuchs endothelial corneal dystrophy (FECD) and the advanced cornea guttae adversely affects the quality of vision, even in patients without corneal edema. The turnover time of cornea guttae is also not certain. Therefore, we used a rabbit model to evaluate the feasibility of Descemet's membrane removal in the optical zone only, by performing a small 4-mm diameter descemetorhexis prior to CEC injection. We showed that the corneal endothelium is regenerated both on the corneal stroma (the area of Descemet's membrane removal) and on the intact peripheral Descemet's membrane, based on the expression of function-related markers and the restoration of corneal transparency. Recovery of the corneal transparency and central corneal thickness was delayed in areas of Descemet's membrane removal, but the cell density of the regenerated corneal endothelium and the thickness of the central corneal did not differ between the areas with and without residual Descemet's membrane at 14 days after CEC injection. Here, we demonstrate that removal of a pathological Descemet's membrane by a small descemetorhexis is a feasible procedure for use in combination with cell-based therapy. The current strategy might be beneficial for improving visual quality after CEC injection as a treatment for FECD.
},
author = {Okumura, Naoki and Matsumoto, Daiki and Fukui, Yuya and Teramoto, Masataka and Imai, Hirofumi and Kurosawa, Tetta and Shimada, Tomoki and Kruse, Friedrich and Schlötzer-Schrehardt, Ursula and Kinoshita, Shigeru and Koizumi, Noriko},
doi = {10.1371/journal.pone.0191306},
faupublication = {yes},
journal = {PLoS ONE},
note = {EVALuna2:34756},
peerreviewed = {Yes},
title = {{Feasibility} of cell-based therapy combined with descemetorhexis for treating {Fuchs} endothelial corneal dystrophy in rabbit model},
volume = {13},
year = {2018}
}
@inproceedings{faucris.228453538,
address = {ROCKVILLE},
author = {Nakayama, Genta and Okumura, Naoki and Oshima, Takeshi and Ueda, Emi and Watanabe, Kyoko and Tourtas, Theofilos and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich and Koizumi, Noriko},
booktitle = {INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE},
date = {2019-04-28/2019-05-02},
faupublication = {yes},
note = {CRIS-Team WoS Importer:2019-10-29},
peerreviewed = {unknown},
publisher = {ASSOC RESEARCH VISION OPHTHALMOLOGY INC},
title = {{Feasibility} of {mTOR} inhibitor for the treatment of {Fuchs} endothelial corneal dystrophy},
venue = {Vancouver, CANADA},
year = {2019}
}
@article{faucris.106523604,
abstract = {The epithelial sodium channel (ENaC) is typically expressed in sodium-absorbing epithelia. Several reports suggest that ENaC is also expressed in ocular tissues and may play a role in aqueous humor secretion and glaucoma. However, the precise localization of ENaC in the human eye is still unclear. Here, the authors studied ENaC expression in 12 normal human donor eyes and in six eyes of patients with glaucoma.Quantitative real-time PCR was used to investigate the expression of ?-, ?-, ?-, and ?-ENaC transcripts in ocular tissues. In addition, the authors performed immunohistochemical studies using recently generated antibodies against human ?- and ?-ENaC.At the mRNA level, all four ENaC subunits were found to be expressed in a wide range of ocular tissues from normal and glaucomatous human eyes, with the cornea, ciliary body, iris, and retina showing the highest expression levels. At the protein level, ?- and ?-ENaC subunits showed distinct distribution patterns and could be immunolocalized primarily to the cell membranes of epithelial cells of the cornea and to the conjunctiva, iris, ciliary body, lens, and retinal pigment epithelium but also to vascular endothelial cells, smooth muscle cells, stromal cells, and retinal neurons. The authors found no altered mRNA level of any subunit in glaucomatous eyes.All four ENaC subunits (????) are expressed in the normal human eye, with distinct localization of subunits possibly reflecting different functional states of the channel. The (patho-)physiological roles of ENaC in the various localizations in the eye remain to be determined.},
author = {Krüger, Bettina and Schlötzer-Schrehardt, Ursula and Härteis, Silke and Zenkel, Matthias and Chankiewitz, Verena E. and Amann, Kerstin Ute and Kruse, Friedrich and Korbmacher, Christoph},
doi = {10.1167/iovs.11-8581},
faupublication = {yes},
journal = {Investigative Ophthalmology & Visual Science},
note = {EVALuna2:4482},
pages = {596-604},
peerreviewed = {Yes},
title = {{Four} subunits (αβγδ) of the epithelial sodium channel ({ENaC}) are expressed in the human eye in various locations},
volume = {53},
year = {2012}
}
@article{faucris.119775304,
abstract = {Limbal stem cell deficiency (LSCD) leads to severe ocular surface abnormalities that can result in the loss of vision. The most successful therapy currently being used is transplantation of limbal epithelial cell sheets cultivated from a limbal biopsy obtained from the patient's healthy, contralateral eye or cadaveric tissue. In this study, we investigated the therapeutic potential of murine vibrissae hair follicle bulge-derived stem cells (HFSCs) as an autologous stem cell (SC) source for ocular surface reconstruction in patients bilaterally affected by LSCD. This study is an expansion of our previously published work showing transdifferentiation of HFSCs into cells of a corneal epithelial phenotype in an in vitro system. In this study, we used a transgenic mouse model, K12(rtTA/rtTA) /tetO-cre/ROSA(mTmG) , which allows for HFSCs to change color, from red to green, once differentiation to corneal epithelial cells occurs and Krt12, the corneal epithelial-specific differentiation marker, is expressed. HFSCs were isolated from transgenic mice, amplified by clonal expansion on a 3T3 feeder layer, and transplanted on a fibrin carrier to the eye of LSCD wild-type mice (n = 31). The HFSC transplant was able to reconstruct the ocular surface in 80% of the transplanted animals; differentiating into cells with a corneal epithelial phenotype, expressing Krt12, and repopulating the corneal SC pool while suppressing vascularization and conjunctival ingrowth. These data highlight the therapeutic properties of using HFSC to treat LSCD in a mouse model while demonstrating a strong translational potential and points to the niche as a key factor for determining stem cell differentiation.},
author = {Meyer-Blazejewska, Ewa Anna and Call, Mindy K. and Yamanaka, Osamu and Liu, Hongshan and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich and Kao, Winston W.},
doi = {10.1002/stem.550},
faupublication = {yes},
journal = {Stem Cells},
note = {EVALuna2:21016},
pages = {57-66},
peerreviewed = {Yes},
title = {{From} hair to cornea: toward the therapeutic use of hair follicle-derived stem cells in the treatment of limbal stem cell deficiency},
volume = {29},
year = {2011}
}
@inproceedings{faucris.228588999,
address = {ROCKVILLE},
author = {Berner, Daniel and Hoja, Ursula and Zenkel, Matthias and Pasutto, Francesca and Lee, Mei Chin and Aung, Tin and Khor, Chiea Chuen and Reis, André and Kruse, Friedrich and Schlötzer-Schrehardt, Ursula},
booktitle = {INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE},
date = {2019-04-28/2019-05-02},
faupublication = {yes},
note = {CRIS-Team WoS Importer:2019-11-01},
peerreviewed = {unknown},
publisher = {ASSOC RESEARCH VISION OPHTHALMOLOGY INC},
title = {{Functional} implication of the pseudoexfoliation-associated rare variant p.{Y407F} at {LOXL1}},
venue = {Vancouver},
year = {2019}
}
@article{faucris.227308597,
abstract = {Purpose To compare the incidence of fungal infection after endothelial keratoplasty (EK) when donor tissue had been stored in hypothermic medium or organ culture. Methods We describe the clinical features of 10 cases of fungal infection (keratitis or endophthalmitis) following EK identified at three European centres. Case definition was the culture of fungus or a positive PCR from the host cornea or anterior chamber after EK. A survey of the incidence of infection after EK was conducted by the European Eye Bank Association. The main outcome measure was the number of cases in which donor tissue had been stored in hypothermic medium compared with organ culture. Results The 10 cases occurred between 2014 and 2017. All donor corneas had been stored in hypothermic medium sourced from three US eye banks. Three pairs of mate corneas caused infections in six recipients. Candida spp were identified from nine cases, with one isolate of Purpureocillium lilacinum. Data on 16 862 corneas supplied for EK were available from 16 European eye banks for the 5-year period from 2012. There were 17 reported cases of infection, of which 15 (88%) were fungal infections and 14 (82%) were Candida spp. Fungal infection was reported from 3 of 14 476 (0.02%) corneas supplied in organ culture compared with 12 of 2386 (0.50%) corneas supplied in hypothermic medium (p<0.0001). The incidence of infection after hypothermic storage was similar for material sourced from Europe (0.52%) or the USA (0.61%). Conclusions Infection after EK is strongly associated with Candida spp. The possible explanations for the higher incidence of infection when tissue is stored in hypothermic medium are discussed.},
author = {Lau, Nicola and Sesé, Aida Hajjar and Augustin, Victor A. and Kuit, Geert and Wilkins, Mark R. and Tourtas, Theofilos and Kruse, Friedrich and Højgaard-Olsen, Klavs and Manuel, Rohini and John Armitage, W. and Larkin, Daniel F. and Tuft, Stephen J.},
doi = {10.1136/bjophthalmol-2018-312709},
faupublication = {yes},
journal = {British Journal of Ophthalmology},
keywords = {cornea; eye (tissue) banking; infection},
note = {CRIS-Team Scopus Importer:2019-10-01},
pages = {1487-1490},
peerreviewed = {Yes},
title = {{Fungal} infection after endothelial keratoplasty: {Association} with hypothermic corneal storage},
volume = {103},
year = {2019}
}
@article{faucris.119551344,
abstract = {A 39-year-old male patient underwent uncomplicated deep anterior lamellar keratoplasty due to keratoconus. On day 5 after surgery, small whitish infiltrates developed in the corneal interface. The diagnosis of fungal keratitis was made when the culture medium of the graft grew Candida after the surgical intervention. Despite intensive antimycotic treatment and irrigation of the interface, the infiltrates persisted and eventually enlarged. Therefore, revision surgery with penetrating keratoplasty was performed. Microbiological analysis showed Candida orthopsilosis in the culture of the excised graft button. Histopathological staining of the excised graft showed periodic acid-Schiff-positive and Grocott methenamine silver-positive clusters of yeast between Descemet's membrane and the deep corneal stroma with focal perforations through Descemet's membrane. The treatment of mycotic keratitis caused by C orthopsilosis is challenging. Antimycotic treatment was unsuccessful in this case. Progression of the keratitis and perforation of Descemet's membrane suggest that early surgical intervention by penetrating keratoplasty is required.},
author = {Wessel, Julia M. and Bachmann, Björn and Meiller, Ralph and Kruse, Friedrich},
doi = {10.1136/bcr-2012-008361},
faupublication = {yes},
journal = {BMJ Case Reports},
note = {EVALuna2:21124},
peerreviewed = {Yes},
title = {{Fungal} interface keratitis by {Candida} orthopsilosis following deep anterior lamellar keratoplasty},
volume = {2013},
year = {2013}
}
@inproceedings{faucris.108225524,
address = {Brno, Czech Republic},
author = {Mayer, Markus Anton and Tornow, Ralf-Peter and Hornegger, Joachim and Kruse, Friedrich},
booktitle = {Analysis of Biomedical Signals and Images, Proceedings of the Biosignal 2008 International Eurasip Conference},
date = {2008-06-29/2008-07-01},
editor = {Jan Jiri, Kozumplik Jiri, Provanznik Ivo},
faupublication = {yes},
pages = {no pagination},
peerreviewed = {unknown},
title = {{Fuzzy} {C}-means {Clustering} {For} {Retinal} {Layer} {Segmentation} {On} {High} {Resolution} {OCT} {Images}},
url = {http://www5.informatik.uni-erlangen.de/Forschung/Publikationen/2008/Mayer08-FCC.pdf},
venue = {Brno},
year = {2008}
}
@article{faucris.118494464,
abstract = {Exfoliation syndrome (XFS) is the most common known risk factor for secondary glaucoma and a major cause of blindness worldwide. Variants in two genes, LOXL1 and CACNA1A, have previously been associated with XFS. To further elucidate the genetic basis of XFS, we collected a global sample of XFS cases to refine the association at LOXL1, which previously showed inconsistent results across populations, and to identify new variants associated with XFS. We identified a rare protective allele at LOXL1 (p.Phe407, odds ratio (OR) = 25, P = 2.9 × 10(-14)) through deep resequencing of XFS cases and controls from nine countries. A genome-wide association study (GWAS) of XFS cases and controls from 24 countries followed by replication in 18 countries identified seven genome-wide significant loci (P < 5 × 10(-8)). We identified association signals at 13q12 (POMP), 11q23.3 (TMEM136), 6p21 (AGPAT1), 3p24 (RBMS3) and 5q23 (near SEMA6A). These findings provide biological insights into the pathology of XFS and highlight a potential role for naturally occurring rare LOXL1 variants in disease biolog},
author = {Aung, Tin and Ozaki, Mineo and Lee, Mei Chin and Schlötzer-Schrehardt, Ursula and Thorleifsson, Gudmar and Mizoguchi, Takanori and Igo, Robert P. and Haripriya, Aravind and Williams, Susan E. and Astakhov, Yury S. and Orr, Andrew C. and Burdon, Kathryn P. and Nakano, Satoko and Mori, Kazuhiko and Abu-Amero, Khaled and Hauser, Michael and Li, Zheng and Prakadeeswari, Gopalakrishnan and Bailey, Jessica N. Cooke and Cherecheanu, Alina Popa and Kang, Jae H. and Nelson, Sarah and Hayashi, Ken and Manabe, Shin-Ichi and Kazama, Shigeyasu and Zarnowski, Tomasz and Inoue, Kenji and Irkec, Murat and Coca-Prados, Miguel and Sugiyama, Kazuhisa and Jarvela, Irma and Schlottmann, Patricio and Lerner, S. Fabian and Lamari, Hasnaa and Nilgun, Yildirim and Bikbov, Mukharram and Park, Ki Ho and Cha, Soon Cheol and Yamashiro, Kenji and Zenteno, Juan C. and Jonas, Jost B. and Kumar, Rajesh S. and Perera, Shamira A. and Chan, Anita S. Y. and Kobakhidze, Nino and George, Ronnie and Vijaya, Lingam and Do, Tan and Edward, Deepak P. and De Juan Marcos, Lourdes and Pakravan, Mohammad and Moghimi, Sasan and Ideta, Ryuichi and Bach-Holm, Daniella and Kappelgaard, Per and Wirostko, Barbara and Thomas, Samuel and Gaston, Daniel and Bedard, Karen and Greer, Wenda L. and Yang, Zhenglin and Chen, Xueyi and Huang, Lulin and Sang, Jinghong and Jia, Hongyan and Jia, Liyun and Qiao, Chunyan and Zhang, Hui and Liu, Xuyang and Zhao, Bowen and Wang, Ya-Xing and Xu, Liang and Leruez, Stephanie and Reynier, Pascal and Chichua, George and Tabagari, Sergo and Uebe, Steffen and Zenkel, Matthias and Berner, Daniel and Mossboeck, Georg and Weisschuh, Nicole and Hoja, Ursula and Welge-Luessen, Ulrich-Christoph and Mardin, Christian Y. and Founti, Panayiota and Chatzikyriakidou, Anthi and Pappas, Theofanis and Anastasopoulos, Eleftherios and Lambropoulos, Alexandros and Ghosh, Arkasubhra and Shetty, Rohit and Porporato, Natalia and Saravanan, Vijayan and Venkatesh, Rengaraj and Shivkumar, Chandrashekaran and Kalpana, Narendran and Sarangapani, Sripriya and Kanavi, Mozhgan R. and Beni, Afsaneh Naderi and Yazdani, Shahin and Lashay, Alireza and Naderifar, Homa and Khatibi, Nassim and Fea, Antonio and Lavia, Carlo and Dallorto, Laura and Rolle, Teresa and Frezzotti, Paolo and Paoli, Daniela and Salvi, Erika and Manunta, Paolo and Mori, Yosai and Miyata, Kazunori and Higashide, Tomomi and Chihara, Etsuo and Ishiko, Satoshi and Yoshida, Akitoshi and Yanagi, Masahide and Kiuchi, Yoshiaki and Ohashi, Tsutomu and Sakurai, Toshiya and Sugimoto, Takako and Chuman, Hideki and Aihara, Makoto and Inatani, Masaru and Miyake, Masahiro and Gotoh, Norimoto and Matsuda, Fumihiko and Yoshimura, Nagahisa and Ikeda, Yoko and Ueno, Morio and Sotozono, Chie and Jeoung, Jin Wook and Sagong, Min and Park, Kyu Hyung and Ahn, Jeeyun and Cruz-Aguilar, Marisa and Ezzouhairi, Sidi M. and Rafei, Abderrahman and Chong, Yaan Fun and Ng, Xiao Yu and Goh, Shuang Ru and Chen, Yueming and Yong, Victor H. K. and Khan, Muhammad Imran and Olawoye, Olusola O. and Ashaye, Adeyinka O. and Ugbede, Idakwo and Onakoya, Adeola and Kizor-Akaraiwe, Nkiru and Teekhasaenee, Chaiwat and Suwan, Yanin and Supakontanasan, Wasu and Okeke, Suhanya and Uche, Nkechi J. and Asimadu, Ifeoma and Ayub, Humaira and Akhtar, Farah and Kosior-Jarecka, Ewa and Lukasik, Urszula and Lischinsky, Ignacio and Castro, Vania and Perez Grossmann, Rodolfo and Megevand, Gordana Sunaric and Roy, Sylvain and Dervan, Edward and Silke, Eoin and Rao, Aparna and Sahay, Priti and Fornero, Pablo and Cuello, Osvaldo and Sivori, Delia and Zompa, Tamara and Mills, Richard A. and Souzeau, Emmanuelle and Mitchell, Paul and Wang, Jie Jin and Hewitt, Alex W. and Coote, Michael and Crowston, Jonathan G. and Astakhov, Sergei Y. and Akopov, Eugeny L. and Emelyanov, Anton and Vysochinskaya, Vera and Kazakbaeva, Gyulli and Fayzrakhmanov, Rinat and Al-Obeidan, Saleh A. and Owaidhah, Ohoud and Aljasim, Leyla Ali and Chowbay, Balram and Foo, Jia Nee and Soh, Raphael Q. and Sim, Kar Seng and Xie, Zhicheng and Cheong, Augustine W. O. and Mok, Shi Qi and Soo, Hui Meng and Chen, Xiao Yin and Peh, Su Qin and Heng, Khai Koon and Husain, Rahat and Ho, Su-Ling and Hillmer, Axel M. and Cheng, Ching-Yu and Escudero-Dominguez, Francisco A. and Gonzalez-Sarmiento, Rogelio and Martinon-Torres, Frederico and Salas, Antonio and Pathanapitoon, Kessara and Hansapinyo, Linda and Wanichwecharugruang, Boonsong and Kitnarong, Naris and Sakuntabhai, Anavaj and Nguyn, Hip X. and Nguyn, Giang T. T. and Nguyn, Trnh V. and Zenz, Werner and Binder, Alexander and Klobassa, Daniela S. and Hibberd, Martin L. and Davila, Sonia and Herms, Stefan and Nothen, Markus M. and Moebus, Susanne and Rautenbach, Robyn M. and Ziskind, Ari and Carmichael, Trevor R. and Ramsay, Michele and Alvarez, Lydia and Garcia, Montserrat and Gonzalez-Iglesias, Hector and Rodriguez-Calvo, Pedro P. and Fernandez-Vega Cueto, Luis and Oguz, Cilingir and Tamcelik, Nevbahar and Atalay, Eray and Batu, Bilge and Aktas, Dilek and Kasim, Burcu and Wilson, M. Roy and Coleman, Anne L. and Liu, Yutao and Challa, Pratap and Herndon, Leon and Kuchtey, Rachel W. and Kuchtey, John and Curtin, Karen and Chaya, Craig J. and Crandall, Alan and Zangwill, Linda M. and Wong, Tien Yin and Nakano, Masakazu and Kinoshita, Shigeru and Den Hollander, Anneke I. and Vesti, Eija and Fingert, John H. and Lee, Richard K. and Sit, Arthur J. and Shingleton, Bradford J. and Wang, Ningli and Cusi, Daniele and Qamar, Raheel and Kraft, Peter and Pericak-Vance, Margaret A. and Raychaudhuri, Soumya and Heegaard, Steffen and Kivela, Tero and Reis, Andre and Kruse, Friedrich and Weinreb, Robert N. and Pasquale, Louis R. and Haines, Jonathan L. and Thorsteinsdottir, Unnur and Jonasson, Fridbert and Allingham, R. Rand and Milea, Dan and Ritch, Robert and Kubota, Toshiaki and Tashiro, Kei and Vithana, Eranga N. and Micheal, Shazia and Topouzis, Fotis and Craig, Jamie E. and Dubina, Michael and Sundaresan, Periasamy and Stefansson, Kari and Wiggs, Janey L. and Pasutto, Francesca and Khor, Chiea Chuen},
doi = {10.1038/ng.3875},
faupublication = {yes},
journal = {Nature Genetics},
note = {EVALuna2:9376},
pages = {993-1004},
peerreviewed = {Yes},
title = {{Genetic} association study of exfoliation syndrome identifies a protective rare variant at {LOXL1} and five new susceptibility loci},
volume = {49},
year = {2017}
}
@article{faucris.119545624,
abstract = {Genetic and nongenetic factors contribute to development of pseudoexfoliation (PEX) syndrome, a complex, age-related, generalized matrix process frequently associated with glaucoma. To identify specific genetic variants underlying its etiology, we performed a genome-wide association study (GWAS) using a DNA-pooling approach. Therefore, equimolar amounts of DNA samples of 80 subjects with PEX syndrome, 80 with PEX glaucoma (PEXG) and 80 controls were combined into separate pools and hybridized to 500K SNP arrays (Affymetrix). Array probe intensity data were analyzed and visualized with expressly developed software tools GPFrontend and GPGraphics in combination with GenePool software. For replication, independent German cohorts of 610 unrelated patients with PEX/PEXG and 364 controls as well as Italian cohorts of 249 patients and 190 controls were used. Of 19, 17 SNPs showing significant allele frequency difference in DNA pools were confirmed by individual genotyping. Further single genotyping at CNTNAP2 locus revealed association between PEX/PEXG for two SNPs, which was confirmed in an independent German but not the Italian cohort. Both SNPs remained significant in the combined German cohorts even after Bonferroni correction (rs2107856: P(c)=0.0108, rs2141388: P(c)=0.0072). CNTNAP2 was found to be ubiquitously expressed in all human ocular tissues, particularly in retina, and localized to cell membranes of epithelial, endothelial, smooth muscle, glial and neuronal cells. Confirming efficiency of GWAS with DNA-pooling approach by detection of the known LOXL1 locus, our study data show evidence for association of CNTNAP2 with PEX syndrome and PEXG in German patients.},
author = {Krumbiegel, Mandy and Pasutto, Francesca and Schlötzer-Schrehardt, Ursula and Uebe, Steffen and Zenkel, Matthias and Mardin, Christian Y. and Weisschuh, Nicole and Paoli, Daniela and Gramer, Eugen and Becker, Christian and Ekici, Arif Bülent and Weber, Bernhard H. F. and Nuernberg, Peter and Kruse, Friedrich and Reis, André},
doi = {10.1038/ejhg.2010.144},
faupublication = {yes},
journal = {European journal of human genetics},
note = {EVALuna2:9084},
pages = {186-93},
peerreviewed = {Yes},
title = {{Genome}-wide association study with {DNA} pooling identifies variants at {CNTNAP2} associated with pseudoexfoliation syndrome},
volume = {19},
year = {2011}
}
@article{faucris.120039084,
abstract = {: To investigate the impact of typical scan score (TSS) on discriminating glaucomatous and healthy eyes by scanning laser polarimetry and spectral domain optical coherence tomography (SD-OCT) in 32 peripapillary sectors.: One hundred two glaucoma patients and 32 healthy controls underwent standard automated perimetry, 24-hour intraocular pressure profile, optic disc photography, GDxVCC, and SD-OCT measurements. For controls, only very typical scans (TSS=100) were accepted. Glaucoma patients were divided into 3 subgroups (very typical: TSS=100; typical: 99>=TSS>=80, atypical: TSS<80). Receiver operating characteristic curves were constructed for mean retinal nerve fiber layer values, sector data, and nerve fiber indicator (NFI). Sensitivity was estimated at >=90% specificity to compare the discriminating ability of each imaging modality.: For discrimination between healthy and glaucomatous eyes with very typical scans, the NFI and inferior sector analyses 26 to 27 demonstrated the highest sensitivity at >=90% specificity in GDxVCC and SD-OCT, respectively. For the typical and atypical groups, sensitivity at >>=90% specificity decreased for all 32 peripapillary sectors on an average by 10.9% and 17.9% for GDxVCC and by 4.9% and 0.8% for SD-OCT. For GDxVCC, diagnostic performance of peripapillary sectors decreased with lower TSS, especially in temporosuperior and inferotemporal sectors (sensitivity at >=90% specificity decreased by 55.3% and by 37.8% in the atypical group).: Diagnostic accuracy is comparable for SD-OCT and GDxVCC if typical scans (TSS=100) are investigated. Decreasing TSS is associated with a decrease in diagnostic accuracy for discriminating healthy and glaucomatous eyes by scanning laser polarimetry. NFI is less influenced than the global or sector retinal nerve fiber layer thickness. The TSS score should be included in the standard printout. Diagnostic accuracy of SD-OCT is barely influenced by low TSS.},
author = {Hoesl, Laura Maria and Tornow, Ralf-Peter and Schrems, Wolfgang and Horn, Folkert and Mardin, Christian Y. and Kruse, Friedrich and Jünemann, Anselm and Lämmer, Robert},
doi = {10.1097/IJG.0b013e318237c8c5},
faupublication = {yes},
journal = {Journal of Glaucoma},
note = {EVALuna2:21037},
pages = {317-24},
peerreviewed = {Yes},
title = {{Glaucoma} {Diagnostic} {Performance} of {GDxVCC} and {Spectralis} {OCT} on {Eyes} {With} {Atypical} {Retardation} {Pattern}},
volume = {22},
year = {2013}
}
@article{faucris.216902176,
abstract = {Purpose: Despite extensive knowledge gained over the last 3 decades regarding limbal stem cell deficiency (LSCD), the disease is not clearly defined, and there is lack of agreement on the diagnostic criteria, staging, and classification system among treating physicians and research scientists working on this field. There is therefore an unmet need to obtain global consensus on the definition, classification, diagnosis, and staging of LSCD.},
author = {Deng, Sophie X. and Borderie, Vincent and Chan, Clara C. and Dana, Reza and Figueiredo, Francisco C. and Gomes, Jose A. P. and Pellegrini, Graziella and Shimmura, Shigeto and Kruse, Friedrich and Cursiefen, Claus and Daya, Sheraz and Djalilian, Ali and Frueh, Beatrice and Hjortdal, Jesper and Holland, Edward and Kaufman, Stephen and Kinoshita, Shigeru and Lee, Barry and Mannis, Mark and Merayo, Jesus and Perez, Victor and Rama, Paolo and Sangwan, Virender and Shortt, Alex and Slomovic, Allan and Solomon, Avi and Tan, Donald and Tsai, Ray and Tseng, Scheffer and Tu, Elmer and Le, Qihua and Jimena, Tatiana C. G. and Sceberras, Virginia and Attico, Eustachio},
doi = {10.1097/ICO.0000000000001820},
faupublication = {yes},
journal = {Cornea},
note = {CRIS-Team WoS Importer:2019-05-03},
pages = {364-375},
peerreviewed = {Yes},
title = {{Global} {Consensus} on {Definition}, {Classification}, {Diagnosis}, and {Staging} of {Limbal} {Stem} {Cell} {Deficiency}},
volume = {38},
year = {2019}
}
@article{faucris.242696974,
abstract = {PURPOSE: In recent decades, the medical and surgical treatment of limbal stem cell deficiency (LSCD) has evolved significantly through the incorporation of innovative pharmacological strategies, surgical techniques, bioengineering, and cell therapy. With such a wide variety of options, there is a need to establish a global consensus on the preferred approaches for the medical and surgical treatment of LSCD. METHODS: An international LSCD Working Group was established by the Cornea Society in 2012 and divided into subcommittees. Four face-to-face meetings, frequent email discussions, and teleconferences were conducted since then to reach agreement on a strategic plan and methods after a comprehensive literature search. A writing group drafted the current study. RESULTS: A consensus in the medical and surgical management of LSCD was reached by the Working Group. Optimization of the ocular surface by eyelid and conjunctival reconstruction, antiinflammatory therapy, dry eye and meibomian gland dysfunction treatment, minimization of ocular surface toxicity from medications, topical medications that promote epithelialization, and use of a scleral lens is considered essential before surgical treatment of LSCD. Depending on the laterality, cause, and stage of LSCD, surgical strategies including conjunctival epitheliectomy, amniotic membrane transplantation, transplantation of limbal stem cells using different techniques and sources (allogeneic vs. autologous vs. ex vivo-cultivated), transplantation of oral mucosal epithelium, and keratoprosthesis can be performed as treatment. A stepwise flowchart for use in treatment decision-making was established. CONCLUSIONS: This global consensus provides an up-to-date and comprehensive framework for the management of LSCD.},
author = {Deng, Sophie X. and Kruse, Friedrich and Gomes, José A.P. and Chan, Clara C. and Daya, Sheraz and Dana, Reza and Figueiredo, Francisco C. and Kinoshita, Shigeru and Rama, Paolo and Sangwan, Virender and Slomovic, Allan R. and Tan, Donald},
doi = {10.1097/ICO.0000000000002358},
faupublication = {yes},
journal = {Cornea},
note = {CRIS-Team Scopus Importer:2020-09-18},
pages = {1291-1302},
peerreviewed = {Yes},
title = {{Global} {Consensus} on the {Management} of {Limbal} {Stem} {Cell} {Deficiency}},
volume = {39},
year = {2020}
}
@article{faucris.122865424,
abstract = {It is essential to devise strategies that improve graft adhesion after Descemet membrane endothelial keratoplasty (DMEK) to reduce the rebubbling rate.To evaluate the influence of the extent of descemetorhexis on graft adhesion properties after DMEK.Single-surgeon, retrospective, observational case series conducted in the Department of Ophthalmology, University of Erlangen-Nuremberg, Germany, that reviewed the medical records of 200 consecutive patients undergoing DMEK. Fifty-three eyes of 51 patients undergoing DMEK for Fuchs endothelial dystrophy fulfilling the inclusion criteria were enrolled in this study. Based on intraoperative drawings, postoperative slitlamp examination, and photographs, eyes were divided into 2 groups. The diameter of the descemetorhexis was approximately 10 mm in group A (30 eyes), resulting in a peripheral 1-mm zone of denuded stroma between the graft and the host's Descemet membrane, and approximately 6 mm in group B (23 eyes), resulting in a peripheral 1-mm zone of overlapping between the graft and the host's Descemet membrane.Graft detachment rate, extent of graft detachment (in clock hours of graft's circumference), and rebubbling rate.Four days after DMEK, the graft detachment rate was 33.3% (10 of 30) in group A and 78.3% (18 of 23) in group B (P = .002). The mean (SD) extent of graft detachment was 0.6 (0.9) and 2.8 (2.5) clock hours in groups A and B, respectively (P < .001), 4 days after surgery. The rebubbling rate was 6.7% (2 of 30) and 30.4% (7 of 23) for groups A and B, respectively (P = .03).A larger descemetorhexis in DMEK is correlated with better graft adhesion and lower rebubbling rates. Therefore, patients with a larger descemetorhexis require less intensive follow-up.},
author = {Tourtas, Theofilos and Schlomberg, Juliane and Wessel, Julia M. and Bachmann, Bjoern O. and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich},
doi = {10.1001/jamaophthalmol.2013.6222},
faupublication = {yes},
journal = {JAMA Ophthalmology},
note = {EVALuna2:21202},
pages = {155-61},
peerreviewed = {Yes},
title = {{Graft} adhesion in descemet membrane endothelial keratoplasty dependent on size of removal of host's descemet membrane},
volume = {132},
year = {2014}
}
@article{faucris.211516706,
abstract = {Stem cells are widely used for numerous clinical applications including limbal stem cell deficiency. Stem cell derived from the bulge region of the hair follicle have the ability to differentiate into a variety of cell types including interfollicular epidermis, hair follicle structures, sebaceous glands and corneal epithelial cells when provided the appropriate cues. Hair follicle stem cells are being studied as a valuable source of autologous stem cells to treat disease. The protocol described below details the isolation and expansion of these cells for eventual clinical application. We used a dual-reporter mouse model to visualize both isolation and eventual differentiation of these cells in a limbal stem cell-deficient mouse model.},
author = {Call, Mindy and Meyer, Ewa Anna and Kao, Winston W. and Kruse, Friedrich and Schlötzer-Schrehardt, Ursula},
doi = {10.21769/BioProtoc.2848},
faupublication = {yes},
journal = {Bio-protocol},
note = {CRIS-Team WoS Importer:2019-02-21},
peerreviewed = {Yes},
title = {{Hair} {Follicle} {Stem} {Cell} {Isolation} and {Expansion}},
volume = {8},
year = {2018}
}
@article{faucris.119651224,
abstract = {Purpose: During deep anterior lamellar keratoplasty (DALK), endothelium and Descemet's membrane are separated from the corneal stroma by intrastromal air injection ('big-bubble technique'). The aim of our study is to analyse histopathological changes in host corneal tissue caused by air insufflation in patients with keratoconus, their variability in 10 patients and their possible clinical implication. Methods: The excised anterior corneal lamellae of 10 patients with keratoconus having undergone DALK using the 'big-bubble technique' were analysed by light and transmission electron microscopy as well as immunohistochemistry. In addition, intrastromal air accumulations were quantified morphometrically. Results: Intrastromal air was detected in all examined excised lamellae (8% of stromal volume), but with large variability (SD 8.8). It was detected preferentially in the inner layer of the corneal stroma and represented there up to 39% of the stromal volume. In addition, the air was predominantly located at one periphery of the excised lamellae. Intrastromal air bubbles were larger in the inner than in the superficial stromal layer and characterized by round shape and a CD68-negative collagenous 'pseudocapsule'. We detected no air-injection-induced alterations in Bowman's layer and epithelium. Conclusion: Our results show that 'big-bubble DALK' causes significant intrastromal air accumulations in the cornea. Pathologists should be conscious of this phenomenon and the high topographic variability. Intrastromal air in the recipient rim may be accompanied by a decrease in mechanical stability and could contribute to postoperative suture loosening.},
author = {Braun, Joachim M. and Hofmann-Rummelt, Carmen and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich and Cursiefen, Claus},
doi = {10.1111/j.1755-3768.2011.02217.x},
faupublication = {yes},
journal = {Acta Ophthalmologica},
note = {EVALuna2:21044},
pages = {78-82},
peerreviewed = {Yes},
title = {{Histopathological} changes after deep anterior lamellar keratoplasty using the 'big-bubble technique'},
volume = {91},
year = {2013}
}
@article{faucris.119765404,
abstract = {To investigate if human anterior lens capsule is a suitable substrate for the culture of primary human trabecular meshwork (HTM) cells. Trabecular meshwork cells derived from four human donors were seeded on anterior lens capsules that were prepared from the lenses of donor eyes. Cell morphology and viability were examined at 1, 3, 5 and 7 days. Cell viability was measured based on a two-colour fluorescence assay (membrane-impermeable propidium iodide and membrane permeable Hoechst 33342). Immunocytochemistry studied Zonula occludens-1 (ZO-1), vimentin, tissue transglutaminase (tTgase) and Na(+)/K(+)-adenosine triphosphatase (Na(+)/K(+)-ATPase). Morphology of the cultivated cells followed a typical model while their viability was > 95% in all cases. ZO-1 was found at the cell boundaries of the HTM-AC complex. Vimentin was located at the lateral membranes of the HTM cells. Na(+)/K(+)-ATPase was found at the basolateral membrane of the HTM cells. tTgase was also identified. Anterior lens capsule can be considered as a suitable alternative substrate for cultivation of HTM cells and assist the expansion of existing knowledge about glaucoma pathophysiology and therapy.},
author = {Kopsachilis, Nikolaos and Tsaousis, Konstantinos and Tsinopoulos, Ioannis T. and Kruse, Friedrich and Welge-Luessen, Ulrich},
doi = {10.1007/s10561-012-9332-2},
faupublication = {yes},
journal = {Cell and Tissue Banking},
note = {EVALuna2:21102},
pages = {407-12},
peerreviewed = {Yes},
title = {{Human} anterior lens capsule serving as a substrate for human trabecular meshwork cells cultivation},
volume = {14},
year = {2013}
}
@article{faucris.273207342,
abstract = {Given their vital role in the homeostasis of the limbal stem cell niche, limbal melanocytes have emerged as promising candidates for tissue engineering applications. This study aimed to isolate and characterize a population of melanocyte precursors in the limbal stroma, compared with melanocytes originating from the limbal epithelium, using magnetic-activated cell sorting (MACS) with positive (CD117/c-Kit microbeads) or negative (CD326/EpCAM or anti-fibroblast microbeads) selection approaches. Both approaches enabled fast and easy isolation and cultivation of pure limbal epithelial and stromal melanocyte populations, which differed in phenotype and gene expression, but exhibited similar functional properties regarding proliferative potential, pigmentation, and support of clonal growth of limbal epithelial stem/progenitor cells (LEPCs). In both melanocyte populations, limbus-specific matrix (laminin 511-E8) and soluble factors (LEPC-derived conditioned medium) stimulated melanocyte adhesion, dendrite formation, melanogenesis, and expression of genes involved in UV protection and immune regulation. The findings provided not only a novel protocol for the enrichment of pure melanocyte populations from limbal tissue applying easy-to-use MACS technology, but also identified a population of stromal melanocyte precursors, which may serve as a reservoir for the replacement of damaged epithelial melanocytes and an alternative resource for tissue engineering applications.},
author = {Li, Shen and Zenkel, Matthias and Kruse, Friedrich and Gießl, Andreas and Schlötzer-Schrehardt, Ursula},
doi = {10.3390/ijms23073756},
faupublication = {yes},
journal = {International Journal of Molecular Sciences},
keywords = {cultivation; laminin 511-E8; limbal stem cell niche; limbal stem cells; limbal stroma; magnetic-activated cell sorting; melanocytes},
note = {CRIS-Team Scopus Importer:2022-04-15},
peerreviewed = {Yes},
title = {{Identification}, {Isolation}, and {Characterization} of {Melanocyte} {Precursor} {Cells} in the {Human} {Limbal} {Stroma}},
volume = {23},
year = {2022}
}
@article{faucris.121964304,
abstract = {Uveal melanomas are the most common malignant tumors of the eye. With modern molecular biological diagnostic methods, such as chromosome 3 typing and gene expression analysis, these tumors can be categorized into highly aggressive (monosomy 3, class II) and less aggressive forms. This molecular biological stratification is primarily important for determining the risk of these tumors as no therapy is currently available that is able to prevent or delay metastases. A randomized study of patients with a poor prognosis (monosomy 3) is currently being carried out in order to determine whether a cancer vaccine prepared from autologous (patient's own) dendritic cells and uveal melanoma RNA can prevent or delay progression and further metastases of this extremely aggressive form of cancer. Inclusion in the uveal melanoma study, which hopes to provide a potential therapeutic option for patients, is only possible if patients are referred to an institution that is able to manufacture and provide this vaccination before the patient is operated on or treated with radiation. Untreated tumor material is necessary for producing the vaccine on an individualized patient basis.},
author = {Schuler-Thurner, Beatrice and Bartz-Schmidt, K. -U. and Bornfeld, N. and Cursiefen, C. and Fuisting, B. and Grisanti, S. and Heindl, L. M. and Holbach, L. and Keserue, M. and Knorr, H. and Koch, K. and Kruse, Friedrich and Meiller, R. and Metz, C. and Meyer-Ter-Vehn, T. and Much, M. and Reinsberg, M. and Schliep, S. and Seitz, B. and Schuler, Gerold and Suesskind, D. and Viestenz, A. and Wagenfeld, L. and Zeschnigk, M.},
doi = {10.1007/s00347-015-0162-z},
faupublication = {yes},
journal = {Ophthalmologe},
note = {EVALuna2:19521},
pages = {1017-21},
peerreviewed = {Yes},
title = {{Immunotherapy} of uveal melanoma: vaccination against cancer : {Multicenter} adjuvant phase 3 vaccination study using dendritic cells laden with tumor {RNA} for large newly diagnosed uveal melanoma},
volume = {112},
year = {2015}
}
@article{faucris.119764524,
abstract = {To investigate the influence of atypical retardation pattern (ARP) on the distribution of peripapillary retinal nerve fibre layer (RNFL) thickness measured with scanning laser polarimetry in healthy individuals and to compare these results with RNFL thickness from spectral domain optical coherence tomography (OCT) in the same subjects.120 healthy subjects were investigated in this study. All volunteers received detailed ophthalmological examination, GDx variable corneal compensation (VCC) and Spectralis-OCT. The subjects were divided into four subgroups according to their typical scan score (TSS): very typical with TSS=100, typical with 99 >= TSS >= 91, less typical with 90 >= TSS >= 81 and atypical with TSS <= 80. Deviations from very typical normal values were calculated for 32 sectors for each group.There was a systematic variation of the RNFL thickness deviation around the optic nerve head in the atypical group for the GDxVCC results. The highest percentage deviation of about 96% appeared temporal with decreasing deviation towards the superior and inferior sectors, and nasal sectors exhibited a deviation of 30%. Percentage deviations from very typical RNFL values decreased with increasing TSS. No systematic variation could be found if the RNFL thickness deviation between different TSS-groups was compared with the OCT results.The ARP has a major impact on the peripapillary RNFL distribution assessed by GDx VCC; thus, the TSS should be included in the standard printout.},
author = {Schrems, Wolfgang and Lämmer, Robert and Hoesl, L. M. and Horn, Folkert and Mardin, C. Y. and Kruse, Friedrich and Tornow, Ralf-Peter},
doi = {10.1136/bjo.2010.190074},
faupublication = {yes},
journal = {British Journal of Ophthalmology},
note = {EVALuna2:21007},
pages = {1437-41},
peerreviewed = {Yes},
title = {{Influence} of atypical retardation pattern on the peripapillary retinal nerve fibre distribution assessed by scanning laser polarimetry and optical coherence tomography},
volume = {95},
year = {2011}
}
@article{faucris.243608659,
abstract = {AIMS: To evaluate the contrast sensitivity in patients with nuclear cataract and corneal guttae compared to patients with nuclear cataract without guttae. METHODS: In this retrospective, single-centre case series, 50 eyes of 50 patients fulfilling the inclusion criteria were enrolled. Patients with corneal guttae and nuclear cataract (n=25, study group) underwent triple Descemet membrane endothelial keratoplasty (DMEK). Patients with nuclear cataract and healthy corneas underwent cataract surgery (n=25, control group). Inclusion criteria were preoperative best-corrected visual acuity ≥20/40, no corneal oedema and similar lens opacity (nuclear opalescence 2.0-2.9). Outcome measures included MARS letter and OPTEC 6500P contrast sensitivity test, corneal volume, central corneal thickness and anterior and posterior corneal densitometry. RESULTS: Preoperative MARS letter and OPTEC 6500P contrast sensitivity was significantly worse in the study group (MARS: p<0.001; OPTEC 6500P: p<0.007 at low spatial frequencies in daylight with and without glare and nightlight without glare). After surgery, there was no significant difference in MARS letter contrast sensitivity between groups (p=0.225). OPTEC 6500P contrast sensitivity remained significantly lower in the study group in daylight and nightlight with and without glare at most spatial frequencies (p<0.01) postoperatively. Preoperative and postoperative corneal volume, central corneal thickness and anterior corneal densitometry were equal in both groups (p>0.05). Posterior densitometry was significantly higher in the study group than in the control group preoperatively (p<0.001) but turned into equal values postoperatively (p=0.07). CONCLUSIONS: Corneal guttae cause an additional significant decrease in contrast sensitivity in eyes with nuclear cataract. This is in favour of performing a triple DMEK even in eyes with a visual acuity of ≥20/40.},
author = {Augustin, Victor A. and Weller, Julia M. and Kruse, Friedrich and Tourtas, Theofilos},
doi = {10.1136/bjophthalmol-2019-315206},
faupublication = {yes},
journal = {British Journal of Ophthalmology},
note = {CRIS-Team Scopus Importer:2020-10-09},
peerreviewed = {Yes},
title = {{Influence} of corneal guttae and nuclear cataract on contrast sensitivity},
year = {2020}
}
@inproceedings{faucris.208857474,
author = {Tourtas, Theofilos and Augustin, Victor A. and Weller, Julia and Kruse, Friedrich},
faupublication = {yes},
note = {EVALuna2:34746},
peerreviewed = {Yes},
title = {{Influence} of {Fuchs} endothelial corneal dystrophy on contrast sensitivity in cataract patients},
volume = {59},
year = {2018}
}
@article{faucris.119425064,
abstract = {The strength of a suture used to attach a graft to a patient can be quantified using the suture retention test. In order to gain deeper insight on the influence of testing and application conditions testing parameters were varied. Different pull rates, suture and jaw positions, and suture materials were tested to show their influence on the result of the test. The results of the different states were analyzed using statistical tests. Based on the statistic analysis conditions for testing with a maximum of accuracy and as close as possible to in vivo conditions have been revealed.},
author = {Küng, Florian and Schubert, Dirk W. and Stafiej, Piotr and Kruse, Friedrich and Fuchsluger, Thomas},
doi = {10.1016/j.msec.2017.02.177},
faupublication = {yes},
journal = {Materials Science and Engineering C},
note = {EVALuna2:21344},
pages = {212-218},
peerreviewed = {Yes},
title = {{Influence} of operating parameters on the suture retention test for scaffolds in ophthalmology},
volume = {77},
year = {2017}
}
@inproceedings{faucris.107135864,
author = {Hehn, Markus and Küng, Florian and Stafiej, Piotr and Kruse, Friedrich and Schubert, Dirk W. and Fuchsluger, Thomas},
booktitle = {15th Bayreuth Polymer Symposium},
faupublication = {yes},
peerreviewed = {unknown},
title = {{Influence} of the molar mass distribution on the suture-ability of nanofiber meshes for tissue engineering},
venue = {Bayreuth},
year = {2017}
}
@inproceedings{faucris.107134324,
author = {Küng, Florian and Schubert, Dirk W. and Stafiej, Piotr and Kruse, Friedrich and Fuchsluger, Thomas},
booktitle = {Association for Research in Vision and Ophthalmology},
date = {2017-05-06/2017-05-11},
faupublication = {yes},
peerreviewed = {unknown},
title = {{Influence} of the molecular weight on the suture retention properties of polycaprolactone},
venue = {Baltimore},
year = {2017}
}
@article{faucris.119975064,
abstract = {To investigate if ultrastructural alterations in the Descemet membrane (DM) are correlated with the clinical outcome after Descemet membrane endothelial keratoplasty (DMEK).Retrospective cohort study.setting: Institutional, single-center.One hundred and twelve residual DM specimens obtained after DM stripping.Incidence of ultrastructural abnormalities in transmission electron microscopy, graft detachment rate, graft failure rate, best-corrected visual acuity (BCVA), endothelial cell density (ECD), and central corneal thickness (CCT). Examination dates were on the day before DMEK and 1, 3, 6, and 12 months after surgery.Abnormalities in the ultrastructure of DM were found in 16 of 112 specimens (14%) (abnormal DM group), comprising deposits of long-spacing collagen, fine filaments (proteoglycans), a posterior collagenous layer, pseudoexfoliative material, and guttae. The secondary graft failure rate was significantly higher in the abnormal DM group compared with the normal DM group (P = .001). There was a trend for an increased graft detachment rate in the abnormal DM group (11/16) compared with the normal DM group (42/96) (P = .103). There was no significant difference in mean CCT and ECD after surgery. Mean CCT in the eyes with graft failure in the abnormal DM group at the last follow-up before regrafting was 850 ?m, indicating endothelial failure with stromal edema.This study reveals a correlation between ultrastructural alterations of DM in donor corneas and the graft failure rate after DMEK. Thus, graft failure after DMEK not only is determined by surgical trauma and postoperative events but may also be influenced by intrinsic, graft-specific features.},
author = {Weller, Julia M. and Schlötzer-Schrehardt, Ursula and Tourtas, Theofilos and Kruse, Friedrich},
doi = {10.1016/j.ajo.2016.06.013},
faupublication = {yes},
journal = {American Journal of Ophthalmology},
note = {EVALuna2:21302},
pages = {58-67},
peerreviewed = {Yes},
title = {{Influence} of {Ultrastructural} {Corneal} {Graft} {Abnormalities} on the {Outcome} of {Descemet} {Membrane} {Endothelial} {Keratoplasty}},
volume = {169},
year = {2016}
}
@article{faucris.208859582,
abstract = {Purpose:We report the occurrence of an extensive submacular hemorrhage after trabeculectomy with mitomycin C in a patient with an occult choroidal neovascular membrane (CNVM).Patients and Methods:A 66-year-old man had a 3-year history of primary open-angle glaucoma in the left eye, which had been treated with topical antiglaucoma medication. The patient had age-related macular degeneration with an occult CNVM, for which he had received 5 intravitreal injections of ranibizumab and 5 intravitreal injections of bevacizumab in the left eye over a 3-year period. As intraocular pressure was not under control in the left eye over a 2-month period, trabeculectomy with mitomycin C was performed.Results:On the first postoperative day, intraocular pressure was 8mmHg with a well-formed bleb in the left eye. However, extensive subretinal hemorrhage was observed, and the patient underwent pneumatic displacement and pars plana vitrectomy to remove the hemorrhage. After 7 months, extensive subretinal fibrosis was observed and visual acuity was low (hand movement only).Conclusions:To our knowledge, this is the first report of an extensive submacular hemorrhage after trabeculectomy with mitomycin C in a patient with an occult CNVM.},
author = {Schrems, Wolfgang and Schrems-Hösl, Laura-Maria and Lämmer, Robert and Kruse, Friedrich and Mardin, Christian Y.},
doi = {10.1097/IJG.0000000000000927},
faupublication = {yes},
journal = {Journal of Glaucoma},
note = {EVALuna2:34752},
pages = {e95-e100},
peerreviewed = {No},
title = {{In} {Reply}: {Precision} of {Optic} {Nerve} {Head} and {Retinal} {Nerve} {Fiber} {Layer} {Parameter} {Measurements} by {Spectral}-domain {Optical} {Coherence} {Tomography}, {Methodological} {Issues} on {Reproducibility}},
volume = {27},
year = {2018}
}
@article{faucris.119765844,
abstract = {We analyzed whether lymphatic vessels can be detected in eyes enucleated after an open globe injury.The presence of lymphatic vessels was analyzed immunohistochemically using podoplanin as a specific lymphatic endothelial marker in 21 globes that had been enucleated after open globe injury. The localization of pathologic lymphatic vessels (within the eye wall or inside the eye) was correlated with the mechanism of trauma, anatomic site of perforation or rupture, and time interval between trauma and enucleation.Pathologic lymphatic vessels were detected in 15 of 21 eyes (71%) enucleated after an open globe injury. In 5 globes (24%) they were found within the eye, located in retrocorneal membranes, underneath the sclera, and adjacent to uveal tissue (ciliary body, iris). No significant association was observed between the presence of pathologic lymphatic vessels and the mechanism of trauma (P = 0.598), anatomic site of perforation or rupture (P = 0.303), and time interval between trauma and enucleation (P = 0.145).The human eye can be invaded secondarily by lymphatic vessels if the eye wall is opened by trauma. This mechanism could be important for wound healing, immunologic defense against intruding microorganisms, and autoimmune reactions against intraocular antigens.},
author = {Wessel, Julia M. and Hofmann-Rummelt, Carmen and Kruse, Friedrich and Cursiefen, Claus and Heindl, Ludwig M.},
doi = {10.1167/iovs.12-9507},
faupublication = {yes},
journal = {Investigative Ophthalmology & Visual Science},
note = {EVALuna2:21111},
pages = {3717-25},
peerreviewed = {Yes},
title = {{Invasion} of lymphatic vessels into the eye after open globe injuries},
volume = {53},
year = {2012}
}
@article{faucris.110680504,
abstract = {To evaluate the efficiency of cultivated human corneal endothelial cells (HCECs) to dehydrate the cornea, using models of the posterior cornea, composed of artificial collagen mass (to represent corneal stroma) and equine collagen membranes (to represent Descemet membrane).HCECs were isolated from donor corneal rings and cultivated at 37°C in 5% CO2 and 95% humidified air. The study design included 4 different sets of models: in set 1, the HCECs were placed directly on the collagen mass complex; in set 2, HCECs were placed on a thin equine collagen membrane and laid over the collagen mass; in set 3, HCECs were placed on a thick equine collagen membrane laid over the collagen mass; and in set 4 (the control group), the hydrophilic collagen mass was left alone to interact with the nutritional medium. The minimum thickness of each sample was measured with optical coherence tomography directly before placement of cells and after exposure to the nutritional fluid for 48 hours.After 2 days of exposure to the nutritional medium, the percentage decreases in thickness in "posterior cornea" models were 66% for set 1, 57% for set 2, and 13% set 3. In the control set, measurement of thickness after 2 days of exposure was not possible because of excessive fluid absorption.This in vitro study of HCECs showed that the dehydrating ability of HCECs is adversely affected by increased thickness of the artificial (Descemet) membrane. Further studies with similar models would aid better understanding of corneal diseases.},
author = {Tsaousis, Konstantinos T. and Kopsachilis, Nikolaos and Tsinopoulos, Ioannis T. and Dimitrakos, Stavros A. and Kruse, Friedrich and Welge-Luessen, Ulrich},
doi = {10.1097/ICO.0000000000000792},
faupublication = {yes},
journal = {Cornea},
note = {EVALuna2:21290},
pages = {669-72},
peerreviewed = {Yes},
title = {{In} {Vitro} {Study} of the {Deturgescence} {Ability} of {Cultivated} {Human} {Corneal} {Endothelial} {Cells}},
volume = {35},
year = {2016}
}
@inproceedings{faucris.228586504,
address = {ROCKVILLE},
author = {Komori, Yuya and Okumura, Naoki and Hanada, Naoya and Tokunaga, Ayumi and Tourtas, Theofilos and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich and Koizumi, Noriko},
booktitle = {INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE},
date = {2019-04-28/2019-05-02},
faupublication = {yes},
note = {CRIS-Team WoS Importer:2019-11-01},
peerreviewed = {unknown},
publisher = {ASSOC RESEARCH VISION OPHTHALMOLOGY INC},
title = {{Involvement} of a disintegrin and metalloproteinase 10 ({ADAM10}) in excessive extracellular matrix production in {Fuchs} endothelial corneal dystrophy},
venue = {Vancouver, CANADA},
year = {2019}
}
@inproceedings{faucris.228587254,
address = {ROCKVILLE},
author = {Tonomura, Shigehito and Okumura, Naoki and Endo, Mako and Nakahara, Makiko and Tourtas, Theofilos and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich and Koizumi, Noriko},
booktitle = {INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE},
date = {2019-04-28/2019-05-02},
faupublication = {yes},
note = {CRIS-Team WoS Importer:2019-11-01},
peerreviewed = {unknown},
publisher = {ASSOC RESEARCH VISION OPHTHALMOLOGY INC},
title = {{Involvement} of caspase 7 in the excessive production of extracellular matrix in {Fuchs} endothelial corneal dystrophy},
venue = {Vancouver, CANADA},
year = {2019}
}
@article{faucris.122073864,
abstract = {Fuchs endothelial corneal dystrophy (FECD) due to corneal endothelial cell degeneration is a major cause of corneal transplantation. It is characterized by abnormal deposition of extracellular matrix (ECM), such as corneal guttae, accompanied by a loss of endothelial cells. Although recent studies have revealed several genomic factors, the molecular pathophysiology of FECD has not yet been revealed. In this study, we establish a cellular in vitro model by using immortalized corneal endothelial cells obtained from late-onset FECD and control patients and examined the involvement of epithelial mesenchymal transition (EMT) on excessive ECM production. We demonstrate that the EMT-inducing genes ZEB1 and SNAI1 were highly expressed in corneal endothelial cells in FECD and were involved in excessive production of ECM proteins, such as type I collagen and fibronectin through the transforming growth factor (TGF)-? signaling pathway. Furthermore, we found that SB431542, a specific inhibitor of TGF-? type I ALK receptors, suppressed the expression of ZEB1 and Snail1 followed by reduced production of ECM. These findings suggest that increased expression levels of ZEB1 and Snail1 in FECD cells were responsible for an increased responsiveness to TGF-? present in the aqueous humor and excessive production of ECM. In addition, these results suggest that the regulation of EMT-related genes by blocking the TGF-? signaling pathway may be a feasible therapeutic strategy for FECD.},
author = {Okumura, Naoki and Minamiyama, Ryuki and Ho, Leona T. Y. and Kay, Eunduck P. and Kawasaki, Satoshi and Tourtas, Theofilos and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich and Young, Robert D. and Quantock, Andrew J. and Kinoshita, Shigeru and Koizumi, Noriko},
doi = {10.1038/labinvest.2015.111},
faupublication = {yes},
journal = {Laboratory Investigation},
note = {EVALuna2:21239},
pages = {1291-304},
peerreviewed = {Yes},
title = {{Involvement} of {ZEB1} and {Snail1} in excessive production of extracellular matrix in {Fuchs} endothelial corneal dystrophy},
volume = {95},
year = {2015}
}
@article{faucris.245478675,
abstract = {Limbal stem cell transplantation has been used successfully to treat patients with limbal stem cell deficiency all over the world. However, long term clinical results often proved less satisfactory due to the low quality of the graft or inadequate properties of transplanted cells. To enhance the ex vivo expansion of human limbal epithelial stem or progenitor cells (LEPC) by preserving stem cell phenotype and to improve subsequent transplantation efficiency, cell-matrix interactions ex vivo should mimic the condition in vivo. The laminin isoforms preferentially expressed in the limbal niche can be used as a culture matrix for epithelial tissue engineering. We recently published the expansion of LEPC on various laminin isoforms and observed that laminin alpha 5-derived matrices support the efficient expansion of LEPC compared to tissue culture plates and other laminin isoforms by preserving stem/progenitor cell phenotype. Here, we describe an optimized protocol for the isolation of LEPC from cadaveric corneal limbal tissue by collagenase digestion and efficient expansion of LEPC using recombinant human laminin-511 E8 fragment (LN-511E8) as culture substrate.},
author = {Polisetti, Naresh and Schlunck, Gunther and Reinhard, Thomas and Kruse, Friedrich and Schlötzer-Schrehardt, Ursula},
doi = {10.21769/BioProtoc.3754},
faupublication = {yes},
journal = {Bio-protocol},
note = {CRIS-Team WoS Importer:2020-11-20},
peerreviewed = {Yes},
title = {{Isolation} and ex vivo {Expansion} of {Human} {Limbal} {Epithelial} {Progenitor} {Cells}},
volume = {10},
year = {2020}
}
@article{faucris.123939244,
abstract = {Optimization of culture conditions for human limbal epithelial stem/progenitor cells (LEPC) that incorporate the in vivo cell-matrix interactions are essential to enhance LEPC ex vivo-expansion and transplantation efficiency. Here, we investigate the efficacy of laminin (LN) isoforms preferentially expressed in the limbal niche as culture matrices for epithelial tissue engineering. Analyses of expression patterns of LN chains in the human limbal niche provided evidence for enrichment of LN-?2, -?3, -?5, -?1, -?2, -?3, -?1, -?2 and -?3 chains in the limbal basement membrane, with LN-?5 representing a signature component specifically produced by epithelial progenitor cells. Recombinant human LN-521 and LN-511 significantly enhanced in vitro LEPC adhesion, migration and proliferation compared to other isoforms, and maintained phenotype stability. The bioactive LN-511-E8 fragment carrying only C-terminal domains showed similar efficacy as full-length LN-511. Functional blocking of ?3?1 and ?6?1 integrins suppressed adhesion of LEPC to LN-511/521-coated surfaces. Cultivation of LEPC on fibrin-based hydrogels incorporating LN-511-E8 resulted in firm integrin-mediated adhesion to the scaffold and well-stratified epithelial constructs, with maintenance of a progenitor cell phenotype in their (supra)basal layers. Thus, the incorporation of chemically defined LN-511-E8 into biosynthetic scaffolds represents a promising approach for xeno-free corneal epithelial tissue engineering for ocular surface reconstruction.},
author = {Polisetti, Naresh and Sorokin, Lydia and Okumura, Naoki and Koizumi, Noriko and Kinoshita, Shigeru and Kruse, Friedrich and Schlötzer-Schrehardt, Ursula},
doi = {10.1038/s41598-017-04916-x},
faupublication = {yes},
journal = {Scientific Reports},
note = {EVALuna2:21349},
pages = {5152},
peerreviewed = {Yes},
title = {{Laminin}-511 and -521-based matrices for efficient ex vivo-expansion of human limbal epithelial progenitor cells},
volume = {7},
year = {2017}
}
@article{faucris.120878164,
abstract = {The purpose of this study was to investigate the usefulness of laminin isoforms as substrates for culturing human corneal endothelial cells (HCECs) for clinical application of tissue engineering therapy.Expression of specific laminin chains in human corneal endothelium and Descemet's membrane was analyzed at the mRNA and protein levels. The effect of laminin-511 and -521 on cell adhesion and proliferation was evaluated. Recombinant laminin E8 fragments (E8s), which represent functionally minimal forms of laminins, were also evaluated for their effects on cell density and cellular phenotype. The potential involvement of ?3?1 and ?6?1 integrins in laminin signal transduction was also investigated using neutralizing antibodies.Laminin-511 and -521 were expressed in Descemet's membrane and corneal endothelium. These laminin isoforms significantly enhanced the in vitro adhesion and proliferation, and differentiation of HCECs. A cell density of 2200 to 2400 cells/mm2 was achieved when HCECs were cultured on laminin-511 or -521, whereas the density was only 1100 cells/mm2 on an uncoated control. E8s also supported HCEC cultivation with a similar efficacy to that obtained with full-length laminin. Functional blocking of ?3?1 and ?6?1 integrins suppressed the adhesion of HCECs even in the presence of laminin-511.Laminin-511 and -521 were the laminin isoforms present in Descemet's membrane, and these laminins modulate the adhesion and proliferation of CECs. Laminin E8s represent an ideal xeno-free defined substrate for the culture of CECs for clinical applications.},
author = {Okumura, Naoki and Kakutani, Kazuya and Numata, Ryohei and Nakahara, Makiko and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich and Kinoshita, Shigeru and Koizumi, Noriko},
doi = {10.1167/iovs.14-15163},
faupublication = {yes},
journal = {Investigative Ophthalmology & Visual Science},
note = {EVALuna2:21243},
pages = {2933-42},
peerreviewed = {Yes},
title = {{Laminin}-511 and -521 enable efficient in vitro expansion of human corneal endothelial cells},
volume = {56},
year = {2015}
}
@article{faucris.240531437,
abstract = {Limbal melanocytes, located in the basal epithelial layer of the corneoscleral limbus, represent essential components of the corneal epithelial stem cell niche, but, due to difficulties in their isolation and cultivation, their biological roles and potential for stem cell-based tissue engineering approaches have not been comprehensively studied. Here, we established a protocol for the efficient isolation and cultivation of pure populations of human limbal melanocytes, which could be expanded at high yield by using recombinant laminin (LN)-511-E8 as culture substrate. Co-cultivation of limbal melanocytes with limbal epithelial stem/progenitor cells on fibrin hydrogels pre-incubated with LN-511-E8 resulted in multilayered stratified epithelial constructs within ten days. By reproducing physiological cell–cell and cell–matrix interactions of the native niche environment, these biomimetic co-culture systems provide a promising experimental model for investigating the functional roles of melanocytes in the limbal stem cell niche and their suitability for developing advanced epithelial grafts for ocular surface surface reconstruction.},
author = {Polisetti, Naresh and Gießl, Andreas and Li, Shen and Sorokin, Lydia and Kruse, Friedrich and Schlötzer-Schrehardt, Ursula},
doi = {10.1038/s41598-020-68120-0},
faupublication = {yes},
journal = {Scientific Reports},
note = {CRIS-Team Scopus Importer:2020-07-17},
peerreviewed = {Yes},
title = {{Laminin}-511-{E8} promotes efficient in vitro expansion of human limbal melanocytes},
volume = {10},
year = {2020}
}
@article{faucris.123567444,
abstract = {To evaluate the incidence of peripheral corneal edema after Descemet membrane endothelial keratoplasty (DMEK) with respect to the size of the descemetorhexis.A single-center retrospective review of data of 200 consecutive DMEK surgeries for Fuchs endothelial dystrophy was performed. Forty-eight eyes of 47 patients were enrolled in this study based on the presence of a peripheral zone of free denuded stroma between the margin of the graft and the host's Descemet membrane (DM) (group A) or a peripheral overlap between the graft and the host's DM (group B). In group A (n=26 eyes), the diameter of the descemetorhexis was approximately 10 mm, whereas in group B (n=22 eyes), the diameter was approximately 6 mm. Both groups received an 8-mm graft. Main outcome measures included peripheral corneal thickness (PCT) at 4 mm from the center, central corneal thickness (CCT), central-to-peripheral thickness ratio (CPTR), and endothelial cell density (ECD).Mean preoperative PCT±SD in group A was 728±52 ?m and in group B was 708±49 ?m (P=0.192). Four weeks after DMEK, mean PCT±SD was 703±43 ?m in group A and 691±59 ?m in group B (P=0.368). Mean preoperative CCT±SD was 642±53 ?m and 627±58 ?m in groups A and B, respectively (P=0.306). There was no significant difference in CCT between groups A and B 4 weeks after surgery (P=0.268). Mean preoperative CPTR±SD in group A was 0.88±0.05 and in group B was 0.89±0.05 (P=0.934). Four weeks after DMEK, CPTR was not significantly different between groups A and B (P=0.893). There was no significant difference in ECD between groups A and B, before and at 4 weeks after DMEK (P=0.093 and P=0.831, respectively).A larger descemetorhexis in DMEK resulting in a peripheral small zone of denuded stroma does not increase the incidence of peripheral corneal edema as compared with a small descemetorhexis with overlapping DMs.},
author = {Tourtas, Theofilos and Weller, Julia M. and Bachmann, Björn and Kruse, Friedrich},
doi = {10.1097/ICL.0000000000000125},
faupublication = {yes},
journal = {Eye & Contact Lens-Science and Clinical Practice},
note = {EVALuna2:21238},
pages = {344-8},
peerreviewed = {Yes},
title = {{Larger} {Descemetorhexis} to {Improve} {Graft} {Adhesion} in {Descemet} {Membrane} {Endothelial} {Keratoplasty} {Does} {Not} {Cause} {Postoperative} {Peripheral} {Corneal} {Edema}},
volume = {41},
year = {2015}
}
@article{faucris.107829964,
abstract = {The aim of this study was to report the clinical, histopathological, and molecular findings in a patient with late-onset lattice corneal dystrophy (LCD) without typical lattice lines and a novel mutation in the TGFBI gene.Corneal lesions were visualized by slit-lamp examination and by in vivo confocal microscopy. Histopathological examination was performed on the patient's corneal specimen obtained during a deep anterior lamellar keratoplasty. By using genomic DNA as a template, all coding regions of the TGFBI gene were amplified and directly sequenced. The presence of the mutation was verified using restriction endonuclease digestion. Eight different computational methods and multiple sequence alignments were used to predict the pathogenicity of the novel genetic variant.The corneal phenotype was characterized by the presence within the stroma of round, oval, and short comma-shaped structures with indistinct margins. Lattice lines were not visible. Histopathological study revealed positive Congo red areas of amyloid deposits typical for LCD. A novel heterozygous missense mutation p.Leu565Pro was identified in exon 13 of the TGFBI gene. The amino acid substitution was unambiguously predicted to have a high pathogenic potential.The mutant codon 565 is located at the C-terminus in the region corresponding to a highly conserved amino acid in the fourth fascilin domain of the TGFBI protein. The novel variant expands the spectrum of TGFBI mutations causing LCD and located in this region. An increased number of known mutations will facilitate future studies of genotype-phenotype correlations and molecular pathogenesis of corneal dystrophies.},
author = {Oldak, Monika and Szaflik, Jacek P. and Sciezynska, Aneta and Udziela, Monika and Maksym, Radoslaw B. and Rymgayllo-Jankowska, Beata and Hofmann-Rummelt, Carmen and Menzel-Severing, Johannes and Ploski, Rafal and Zarnowski, Tomasz and Kruse, Friedrich and Szaflik, Jerzy},
doi = {10.1097/ICO.0000000000000062},
faupublication = {yes},
journal = {Cornea},
note = {EVALuna2:21208},
pages = {294-9},
peerreviewed = {Yes},
title = {{Late}-onset lattice corneal dystrophy without typical lattice lines caused by a novel mutation in the {TGFBI} gene},
volume = {33},
year = {2014}
}
@article{faucris.119552884,
abstract = {Trace elements might play a role in the complex multifactorial pathogenesis of age-related macular degeneration (AMD). The aim of this study was to measure alterations of trace elements levels in aqueous humor of patients with non-exsudative (dry) AMD.For this pilot study, aqueous humor samples were collected from patients undergoing cataract surgery. 12 patients with dry AMD (age 77.9±6.62, female 8, male 4) and 11 patients without AMD (age 66.6±16.7, female 7, male 4) were included. Aqueous levels of cadmium, cobalt, copper, iron, manganese, selenium, and zinc were measured by use of Flow-Injection-Inductively-Coupled-Plasma-Mass-Spectrometry (FI-ICP-MS), quality controlled with certified standards.PATIENTS WITH AMD HAD SIGNIFICANTLY HIGHER AQUEOUS HUMOR LEVELS OF CADMIUM (MEDIAN: 0.70 µmol/L, IQR: 0.40-0.84 vs. 0.06 µmol/L; IQR: 0.01-.018; p = 0.002), cobalt (median: 3.1 µmol/L, IQR: 2.62-3.15 vs. 1.17 µmol/L; IQR: 0.95-1.27; p<0.001), iron (median: 311 µmol/L, IQR: 289-329 vs. 129 µmol/L; IQR: 111-145; p<0.001) and zinc (median: 23.1 µmol/L, IQR: 12.9-32.6 vs. 5.1 µmol/L; IQR: 4.4-9.4; p = 0.020) when compared with patients without AMD. Copper levels were significantly reduced in patients with AMD (median: 16.2 µmol/L, IQR: 11.4-31.3 vs. 49.9 µmol/L; IQR: 32.0-.142.0; p = 0.022) when compared to those without. No significant differences were observed in aqueous humor levels of manganese and selenium between patients with and without AMD. After an adjustment for multiple testing, cadmium, cobalt, copper and iron remained a significant factor in GLM models (adjusted for age and gender of the patients) for AMD.Alterations of trace element levels support the hypothesis that cadmium, cobalt, iron, and copper are involved in the pathogenesis of AMD.},
author = {Jünemann, Anselm and Stopa, Piotr and Michalke, Bernhard and Chaudhri, Anwar and Reulbach, Udo and Huchzermeyer, Cord and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich and Zrenner, Eberhart and Rejdak, Robert},
doi = {10.1371/journal.pone.0056734},
faupublication = {yes},
journal = {PLoS ONE},
note = {EVALuna2:21116},
pages = {e56734},
peerreviewed = {Yes},
title = {{Levels} of {Aqueous} {Humor} {Trace} {Elements} in {Patients} with {Non}-{Exsudative} {Age}-related {Macular} {Degeneration}: {A} {Case}-control {Study}},
volume = {8},
year = {2013}
}
@article{faucris.106677164,
abstract = {Regeneration and repair of corneal epithelium rely on a reservoir of unipotent progenitor cells, which is situated within the basal epithelial layer at the corneoscleral limbus. If these cells are lost, corneal surface integrity is disturbed, which may lead to a painful loss of vision. Since the late 1990s cultivated grafts of limbal epithelium are being used therapeutically. Limbal epithelial cells are obtained from the fellow eye or from an allogeneic donor, propagated in culture on different types of carriers, and subsequently transplanted. This process entails removal of progenitor cells from their natural environment. However, surrounding cells and extracellular matrix are widely believed to provide important stimuli for stem cell maintenance and for correct differentiation. Therefore, new approaches aim at providing this so-called stem cell niche ex vivo and following transplantation. Niche factors can also drive transdifferentiation of alternative progenitor cell types towards a corneal phenotype. This permits the use of autologous cells in cases of bilateral limbal stem cell insufficiency. Several biosynthetic substrates have been devised for culture, transdifferentiation and transplantation of donor cells. This work intends to provide an overview of constructs that are currently available and to some extent clinically employed. In addition, a summary is given of novel concepts which aim at integrating putative niche factors into the stem cell carriers to replicate the stem cell niche.},
author = {Menzel-Severing, Johannes and Polisetti, N. and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich},
doi = {10.1055/s-0032-1315378},
faupublication = {yes},
journal = {Klinische Monatsblätter für Augenheilkunde},
note = {EVALuna2:21097},
pages = {1191-7},
peerreviewed = {Yes},
title = {{Limbal} {Stem} {Cells} and their {Niche}: {Implications} for {Bioengineered} {Tissue} {Constructs}},
volume = {229},
year = {2012}
}
@article{faucris.277810049,
abstract = {Long COVID (LC) describes the clinical phenotype of symptoms after infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Diagnostic and therapeutic options are limited, as the pathomechanism of LC is elusive. As the number of acute SARS-CoV-2 infections was and is large, LC will be a challenge for the healthcare system. Previous studies revealed an impaired blood flow, the formation of microclots, and autoimmune mechanisms as potential factors in this complex interplay. Since functionally active autoantibodies against G-protein-coupled receptors (GPCR-AAbs) were observed in patients after SARS-CoV-2 infection, this study aimed to correlate the appearance of GPCR-AAbs with capillary microcirculation. The seropositivity of GPCR-AAbs was measured by an established cardiomyocyte bioassay in 42 patients with LC and 6 controls. Retinal microcirculation was measured by OCT–angiography and quantified as macula and peripapillary vessel density (VD) by the Erlangen-Angio Tool. A statistical analysis yielded impaired VD in patients with LC compared to the controls, which was accentuated in female persons. A significant decrease in macula and peripapillary VD for AAbs targeting adrenergic β2-receptor, MAS-receptor angiotensin-II-type-1 receptor, and adrenergic α1-receptor were observed. The present study might suggest that a seropositivity of GPCR-AAbs can be linked to an impaired retinal capillary microcirculation, potentially mirroring the systemic microcirculation with consecutive clinical symptoms.},
author = {Szewczykowski, Charlotte and Mardin, Christian and Lucio, Marianna and Wallukat, Gerd and Hoffmanns, Jakob and Schröder, Tim and Raith, Franziska and Rogge, Lennart and Heltmann, Felix and Moritz, Michael and Beitlich, Lorenz and Schottenhamml, Julia and Herrmann, Martin and Harrer, Thomas and Ganslmayer, Marion and Kruse, Friedrich and Kräter, Martin and Lämmer, Robert and Zenkel, Matthias and Gießl, Andreas and Hohberger, Bettina and Guck, Jochen},
doi = {10.3390/ijms23137209},
faupublication = {yes},
journal = {International Journal of Molecular Sciences},
keywords = {chronic fatigue syndrome; COVID-19; functionally GPCR autoantibodies; glaucoma; Long-COVID syndrome; OCT–angiography},
note = {CRIS-Team Scopus Importer:2022-07-15},
peerreviewed = {Yes},
title = {{Long} {COVID}: {Association} of {Functional} {Autoantibodies} against {G}-{Protein}-{Coupled} {Receptors} with an {Impaired} {Retinal} {Microcirculation}},
volume = {23},
year = {2022}
}
@article{faucris.119598204,
abstract = {To compare the longitudinal loss of RNFL thickness measurements by SD-OCT in healthy individuals and glaucoma patients with or without progression concerning optic disc morphology.A total of 62 eyes, comprising 38 glaucomatous eyes with open angle glaucoma and 24 healthy controls, were included in the study (Erlangen Glaucoma Registry, NTC00494923). All patients were investigated annually over a period of 3 years by Spectralis SD-OCT measuring peripapillary RNFL thickness. By masked comparative analysis of photographs, the eyes were classified into nonprogressive and progressive glaucoma cases. Longitudinal loss of RNFL thickness was compared with morphological changes of optic disc morphology.Mixed model analysis of annual OCT scans revealed an estimated annual decrease of the RNFL thickness by 2.12 ?m in glaucoma eyes with progression, whereas glaucoma eyes without progression in optic disc morphology lost 1.18 ?m per year in RNFL thickness (P = 0.002). The rate of change in healthy eyes was 0.60 ?m and thereby also significantly lower than in glaucoma eyes with progression (P < 0.001). The intrasession variability of three successive measurements without head repositioning was 1.5 ± 0.7 ?m. The loss of mean RNFL thickness exceeded the intrasession variability in 60% of nonprogressive eyes, and in 85% of progressive eyes after 3 years.LONGITUDINAL MEASUREMENTS OF RNFL THICKNESS USING SD-OCT SHOW A MORE PRONOUNCED REDUCTION OF RNFL THICKNESS IN PATIENTS WITH PROGRESSION COMPARED WITH PATIENTS WITHOUT PROGRESSION IN GLAUCOMATOUS OPTIC DISC CHANGES. (www.clinicaltrials.gov number, NTC00494923.).},
author = {Wessel, Julia M. and Horn, Folkert and Tornow, Ralf-Peter and Schmid, Matthias and Mardin, Christian Y. and Kruse, Friedrich and Jünemann, Anselm and Lämmer, Robert},
doi = {10.1167/iovs.12-9786},
faupublication = {yes},
journal = {Investigative Ophthalmology & Visual Science},
note = {EVALuna2:5384},
pages = {3613-20},
peerreviewed = {Yes},
title = {{Longitudinal} analysis of progression in glaucoma using spectral-domain optical coherence tomography},
volume = {54},
year = {2013}
}
@article{faucris.120826244,
abstract = {To evaluate the long-term clinical outcome up to 5 years after Descemet membrane endothelial keratoplasty (DMEK).Retrospective, consecutive case series.In this single-center study, 310 consecutive DMEK operations for endothelial decompensation were reviewed; 97 eyes of 84 patients met the inclusion criterion of a minimum 3-year follow-up. Retrospective evaluation of clinical examinations occurred at 1 and 3 months and annually up to 5 years after DMEK at the Department of Ophthalmology, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen (FAU), Germany. Main outcome measures were corrected distance visual acuity (CDVA), endothelial cell density (ECD), central corneal thickness (CCT), and graft survival (Kaplan-Meier analysis).Mean follow-up was 53 ± 13 months. CDVA improved from 0.62 ± 0.42 logMAR before DMEK to 0.13 ± 0.12 logMAR (P < .001); 57% of eyes without ocular comorbidities reached >=20/25 at 5 years after DMEK. ECD was stable after the initial postsurgical decrease (42% at 1 month, 44% at 5 years), from 2602 ± 243 cells/mm(2) before DMEK to 1460 ± 179 cells/mm(2) at 5 years. CCT decreased from 644 ± 67 ?m before DMEK to 557 ± 49 ?m at 5 years, with a minimum (530 ± 54 ?m) at 3 months. Cumulative probability of 5-year graft survival was 95%.The long-term sustainability of DMEK was confirmed. DMEK not only provides fast visual rehabilitation but maintains its clinical outcome within a follow-up of 5 years. Visual acuity and endothelial cell loss remain stable between 3 months and 5 years after DMEK.},
author = {Schlögl, Andreas and Tourtas, Theofilos and Kruse, Friedrich and Weller, Julia M.},
doi = {10.1016/j.ajo.2016.07.002},
faupublication = {yes},
journal = {American Journal of Ophthalmology},
note = {EVALuna2:21324},
pages = {218-26},
peerreviewed = {Yes},
title = {{Long}-term {Clinical} {Outcome} {After} {Descemet} {Membrane} {Endothelial} {Keratoplasty}},
volume = {169},
year = {2016}
}
@article{faucris.225628561,
author = {Jünemann, Anselm and Bellios, N. and Lämmer, Robert and Link, B. and Mardin, C. Y. and Kremers, Jan and Kruse, Friedrich and Horn, Folkert},
faupublication = {yes},
journal = {Investigative Ophthalmology & Visual Science},
keywords = {visual fields},
peerreviewed = {Yes},
title = {{Long} {Term} {Follow}-{Up} of {Preperimetric} {Open}-{Angle} {Glaucoma}: {Improvement} of the {Visual} {Field} in {Morphological} {Stable} {Patients} {Only}},
volume = {51},
year = {2010}
}
@article{faucris.257697477,
abstract = {Purpose: Radiotherapy represents an effective treatment option in Graves’ ophthalmopathy (GO), leading to palliation of clinical symptoms. However, there are only a limited number of trials comparing the effectiveness of low- vs. high-dose radiotherapy. Methods: We analyzed 127 patients treated with radiotherapy for stage 3/4 GO (NOSPECS classification). Patients were treated with single doses of 2.0 Gy (cumulative dose 20 Gy) until 2007, afterwards a single dose of 0.8 Gy (cumulative dose 4.8 Gy) was applied. With a median follow-up-time of 9.0 years, the treatment efficacy (overall improvement, sense of eye pressure, lid edema, ocular motility, exophthalmos, subjective vision, and diplopia) and adverse effects were analyzed by a standardized survey. Results: Overall, 63.8% described improvement of symptoms after radiotherapy. No significant differences in overall treatment response and improvement of main outcome measures between low- or high-dose radiotherapy treatments are detectable, while low-dose radiotherapy leads significantly more often to retreatment (13.1% vs. 1.7%, p = 0.016). The main independent predictor of treatment response is the presence of lid edema (odds ratio, OR, 3.53; p = 0.006). Conclusion: At long-term follow-up, the majority of patients reported palliation of symptoms with limited adverse effects, suggesting clinical effectiveness of radiotherapy for amelioration of GO symptoms independent of low- or high-dose radiotherapy.},
author = {Weissmann, Thomas and Lettmaier, Sebastian and Donaubauer, Anna-Jasmina and Bert, Christoph and Schmidt, Manfred and Kruse, Friedrich and Ott, Oliver and Hecht, Markus and Fietkau, Rainer and Frey, Benjamin and Putz, Florian},
doi = {10.1007/s00066-021-01770-9},
faupublication = {yes},
journal = {Strahlentherapie und Onkologie},
keywords = {Exophthalmos; Graves’ ophthalmopathy; Low dose radiation therapy; Radiotherapy; Thyroid eye disease},
note = {CRIS-Team Scopus Importer:2021-05-07},
peerreviewed = {Yes},
title = {{Low}- vs. high-dose radiotherapy in {Graves}’ ophthalmopathy: a retrospective comparison of long-term results},
year = {2021}
}
@article{faucris.119553764,
abstract = {To test the hypothesis that a primary disturbance in lysyl oxidase-like 1 (LOXL1) and elastin metabolism in the lamina cribrosa of eyes with pseudoexfoliation syndrome constitutes an independent risk factor for glaucoma development and progression.Observational, consecutive case series.Posterior segment tissues obtained from 37 donors with early and late stages of pseudoexfoliation syndrome without glaucoma, 37 normal age-matched control subjects, 5 eyes with pseudoexfoliation-associated open-angle glaucoma, and 5 eyes with primary open-angle glaucoma (POAG).Protein and mRNA expression of major elastic fiber components (elastin, fibrillin-1, fibulin-4), collagens (types I, III, and IV), and lysyl oxidase crosslinking enzymes (LOX, LOXL1, LOXL2) were assessed in situ by quantitative real-time polymerase chain reaction, (immuno)histochemistry, and light and electron microscopy. Lysyl oxidase-dependent elastin fiber assembly was assessed by primary optic nerve head astrocytes in vitro.Expression levels of elastic proteins, collagens, and lysyl oxidases in the lamina cribrosa.Lysyl oxidase-like 1 proved to be the major lysyl oxidase isoform in the normal lamina cribrosa in association with a complex elastic fiber network. Compared with normal and POAG specimens, lamina cribrosa tissues obtained from early and late stages of pseudoexfoliation syndrome without and with glaucoma consistently revealed a significant coordinated downregulation of LOXL1 and elastic fiber constituents on mRNA and protein level. In contrast, expression levels of collagens and other lysyl oxidase isoforms were not affected. Dysregulated expression of LOXL1 and elastic proteins was associated with pronounced (ultra)structural alterations of the elastic fiber network in the laminar beams of pseudoexfoliation syndrome eyes. Inhibition of LOXL1 interfered with elastic fiber assembly by optic nerve head astrocytes in vitro.The findings provide evidence for a pseudoexfoliation-specific elastinopathy of the lamina cribrosa resulting from a primary disturbance in LOXL1 regulation and elastic fiber homeostasis, possibly rendering pseudoexfoliation syndrome eyes more vulnerable to pressure-induced optic nerve damage and glaucoma development and progression.},
author = {Schlötzer-Schrehardt, Ursula and Hammer, Christian and Krysta, Anita W. and Hofmann-Rummelt, Carmen and Pasutto, Francesca and Sasaki, Takako and Kruse, Friedrich and Zenkel, Matthias},
doi = {10.1016/j.ophtha.2012.03.015},
faupublication = {yes},
journal = {Ophthalmology},
note = {EVALuna2:1645},
pages = {1832-43},
peerreviewed = {Yes},
title = {{LOXL1} deficiency in the lamina cribrosa as candidate susceptibility factor for a pseudoexfoliation-specific risk of glaucoma},
volume = {119},
year = {2012}
}
@article{faucris.285663703,
abstract = {Purpose:The purpose of this study was to describe the feasibility of Descemet membrane endothelial keratoplasty (DMEK) as a treatment modality for spontaneous detachment of DM (DMD) decades after penetrating keratoplasty (PK) for keratoconus.Methods:We describe the clinical characteristics and therapeutic surgical approach in 6 eyes of 5 patients with DMD. Clinical images, anterior segment optical coherence tomography scans, and histological findings are presented.Results:Mean age of patients at time of diagnosis was 60 years (range 56-66 years). Mean interval between PK and occurrence of DM detachment was 36 years (range 29-45 years). In 4 of 6 eyes, air injections into the anterior chamber were initially attempted to reattach DM to the stroma but without long-lasting effect. Two eyes underwent repeat PK because of pronounced ectasia after long-standing DMD and stromal scars. DMEK was performed successfully in 4 eyes leading to an increase in visual acuity and a reduction in central corneal thickness. Electron microscopy showed abnormal vacuolar inclusions and collagenous material in the posterior nonbanded layer and a separation of the anterior banded layer from the posterior nonbanded layer.Conclusions:This case series provides evidence that DMEK is a viable option in eyes with spontaneous DM detachment after PK. Visual outcome is limited by the persisting high astigmatism in the ectatic cornea. Illustrated by a small series of patients, the results of DMEK in this condition are presented and new findings about the pathophysiology are given.},
author = {Weller, Julia and Kruse, Friedrich and Schlötzer-Schrehardt, Ursula and Tourtas, Theofilos},
doi = {10.1097/ICO.0000000000003027},
faupublication = {yes},
journal = {Cornea},
keywords = {Descemet membrane detachment; DMEK; keratoconus},
note = {CRIS-Team Scopus Importer:2022-11-25},
pages = {1503-1511},
peerreviewed = {Yes},
title = {{Management} of {Late} {Descemet}'s {Membrane} {Detachment} after {Penetrating} {Keratoplasty} in {Keratoconus}},
volume = {41},
year = {2022}
}
@inproceedings{faucris.107136744,
author = {Regelmann, Christian and Küng, Florian and Stafiej, Piotr and Kruse, Friedrich and Schubert, Dirk W. and Fuchsluger, Thomas},
booktitle = {15th Bayreuth Polymer Symposium},
faupublication = {yes},
peerreviewed = {unknown},
title = {{Mapping} of the electrospinning parameters for {PLGA}-{Nanofibers}},
venue = {Bayreuth},
year = {2017}
}
@inproceedings{faucris.207810010,
author = {Schlötzer-Schrehardt, Ursula and Polisetti, Naresh and Zenkel, Matthias and Naschberger, Elisabeth and Heger, Lukas and Dudziak, Diana and Stürzl, Michael and Kruse, Friedrich},
faupublication = {yes},
note = {EVALuna2:34738},
peerreviewed = {Yes},
title = {{Melanocytes} as an emerging key player in niche regulation of limbal stem cells},
volume = {59},
year = {2018}
}
@article{faucris.263533614,
abstract = {Purpose: Limbal melanocytes (LMel) represent essential components of the corneal epithelial stem cell niche and are known to protect limbal epithelial stem/progenitor cells (LEPCs) from UV damage by transfer of melanosomes. Here, we explored additional functional roles for LMel in niche homeostasis, immune regulation and angiostasis. Methods: Human corneoscleral tissues were morphologically analyzed in normal, inflammatory and wound healing conditions. The effects of LMel on LEPCs were analyzed in direct and indirect co-culture models using electron microscopy, immunocytochemistry, qRT-PCR, Western blotting and functional assays; limbal mesenchymal stromal cells and murine embryonic 3T3 fibroblasts served as controls. The immunophenotype of LMel was assessed by flow cytometry before and after interferon-γ stimulation, and their immunomodulatory properties were analyzed by mixed lymphocytes reaction, monocyte adhesion assays and cytometric bead arrays. Their angiostatic effects on human umbilical cord endothelial cells (HUVECs) were evaluated by proliferation, migration, and tube formation assays. Results: LMel and LEPCs formed structural units in the human limbal stem cell niche in situ, which could be functionally replicated, including melanosome transfer, by co-cultivation in vitro. LMel supported LEPCs during clonal expansion and during epithelial wound healing by stimulating proliferation and migration, and suppressed their differentiation through direct contact and paracrine effects. Under inflammatory conditions, LMel were increased in numbers and upregulated expression of ICAM-1 and MHC II molecules (HLA-DR), but lacked expression of HLA-G, -DP, -DQ and costimulatory molecules CD80 and CD86. They were also found to be potent suppressors of alloreactive T- cell proliferation and cytokine secretion, which largely depended on direct cell-cell interaction. Moreover, the LMel secretome exerted angiostatic activity by inhibiting vascular endothelial cell proliferation and capillary network formation. Conclusion: These findings suggest that LMel are not only professional melanin-producing cells, but exert various non-canonical functions in limbal niche homeostasis by regulating LEPC maintenance, immune responses, and angiostasis. Their potent regulatory, immunomodulatory and anti-angiogenic properties may have important implications for future regenerative cell therapies.},
author = {Polisetti, Naresh and Gießl, Andreas and Zenkel, Matthias and Heger, Lukas and Dudziak, Diana and Naschberger, Elisabeth and Stich, Lena and Steinkasserer, Alexander and Kruse, Friedrich and Schlötzer-Schrehardt, Ursula},
doi = {10.1016/j.jtos.2021.08.006},
faupublication = {yes},
journal = {Ocular Surface},
keywords = {Angiostasis; Immune regulation; Inflammation; Limbal niche; Limbal stem cells; Melanocytes; Wound healing},
note = {CRIS-Team Scopus Importer:2021-09-03},
pages = {172-189},
peerreviewed = {Yes},
title = {{Melanocytes} as emerging key players in niche regulation of limbal epithelial stem cells},
volume = {22},
year = {2021}
}
@article{faucris.119915884,
abstract = {To describe a new surgical technique allowing dissection down to Descemet membrane in big-bubble deep anterior lamellar keratoplasty (DALK) with failed big-bubble formation (the "microbubble incision technique").This is an interventional case series of 10 consecutive patients with keratoconus undergoing intended big-bubble DALK with failure to establish a normal big bubble. In all patients, repeated air injections into the stroma were performed, leaving a whitish colored stroma. Lamellar dissection as far down as possible was then performed within this white tissue. As soon as the anterior chamber was visible, a large remaining intrastromal air bubble was incised with a sharp 15-degree knife introduced perpendicular to the tissue to open up this predescemetic bubble. If deeper air bubbles were still visible, this approach was repeated. Using a blunt spatula, this new layer was then prepared and viscodissection performed.Using this novel approach, in 9 of the 10 patients, it was possible to dissect down to Descemet membrane. Macroperforation made conversion to penetrating keratoplasty necessary in 1 patient. Microperforations not necessitating conversion occurred in 2 patients. All 9 patients with "rescued" DALK had an uneventful postoperative course and had a mean visual acuity of 20/63 ± 20/125 (range, 20/500-20/50) and a mean endothelial cell count of 1672 ± 163 cells per square millimeter (range, 1493-1867 cells/mm) at 3 months.Microbubble incision is a new rescue technique for big-bubble DALK patients without bubble formation allowing for a safer dissection down to Descemet membrane.},
author = {Riss, Stephan and Heindl, Ludwig M. and Bachmann, Bjoern O. and Kruse, Friedrich and Cursiefen, Claus},
doi = {10.1097/ICO.0b013e31824a226f},
faupublication = {yes},
journal = {Cornea},
note = {EVALuna2:21090},
pages = {125-9},
peerreviewed = {Yes},
title = {{Microbubble} incision as a new rescue technique for big-bubble deep anterior lamellar keratoplasty with failed bubble formation},
volume = {32},
year = {2013}
}
@article{faucris.119385464,
abstract = {To characterize changes in the energy-producing metabolic activity and morphologic ultrastructure of corneal endothelial cells associated with diabetes mellitus.Transplant suitable corneoscleral tissue was obtained from donors aged 50 to 75 years. We assayed 3-mm punches of endothelium-Descemet membrane for mitochondrial respiration and glycolysis activity using extracellular flux analysis of oxygen and pH, respectively. Transmission electron microscopy was used to assess qualitative and quantitative ultrastructural changes in corneal endothelial cells and associated Descemet membrane. For purposes of analysis, samples were divided into four groups based on a medical history of diabetes regardless of type: (1) nondiabetic, (2) noninsulin-dependent diabetic, (3) insulin-dependent diabetic, and (4) insulin-dependent diabetic with specified complications due to diabetes (advanced diabetic).In total, 229 corneas from 159 donors were analyzed. Insulin-dependent diabetic samples with complications due to diabetes displayed the lowest spare respiratory values compared to all other groups (P <= 0.002). The remaining mitochondrial respiration and glycolysis metrics did not differ significantly among groups. Compared to nondiabetic controls, the endothelium from advanced diabetic samples had alterations in mitochondrial morphology, pronounced Golgi bodies associated with abundant vesicles, accumulation of lysosomal bodies/autophagosomes, and focal production of abnormal long-spacing collagen.Extracellular flux analysis suggests that corneal endothelial cells of donors with advanced diabetes have impaired mitochondrial function. Metabolic findings are supported by observed differences in mitochondrial morphology of advanced diabetic samples but not controls. Additional studies are needed to determine the precise mechanism(s) by which mitochondria become impaired in diabetic corneal endothelial cells.},
author = {Aldrich, Benjamin T. and Schlötzer-Schrehardt, Ursula and Skeie, Jessica M. and Burckart, Kimberlee A. and Schmidt, Gregory A. and Reed, Cynthia R. and Zimmerman, M. Bridget and Kruse, Friedrich and Greiner, Mark A.},
doi = {10.1167/iovs.16-21094},
faupublication = {yes},
journal = {Investigative Ophthalmology & Visual Science},
note = {EVALuna2:21336},
pages = {2130-2138},
peerreviewed = {Yes},
title = {{Mitochondrial} and {Morphologic} {Alterations} in {Native} {Human} {Corneal} {Endothelial} {Cells} {Associated} {With} {Diabetes} {Mellitus}},
volume = {58},
year = {2017}
}
@article{faucris.211516346,
abstract = {The goal of this protocol is to establish a procedure for cultivating stem cells on a fibrin carrier to allow for eventual transplantation to the eye. The ability to transfer stem cells to a patient is critical for treatment for a variety of disorders and wound repair. We took hair follicle stem cells from the vibrissae of transgenic mice expressing a dual reporter gene under the control of the Tet-on system and the keratin 12 promoter (Meyer-Blazejewska et al., 2011). A clonal growth assay was performed to enrich for stem cells. Once holoclones formed they were transferred onto a fibrin carrier and cultivated to obtain a confluent epithelial cell layer. Limbal stem cell deficient (LSCD) mice were used as the transplant recipient in order to test for successful grafting and eventual differentiation into a corneal epithelial phenotype.},
author = {Call, Mindy and Meyer, Ewa Anna and Kao, Winston W. and Kruse, Friedrich and Schlötzer-Schrehardt, Ursula},
doi = {10.21769/BioProtoc.2849},
faupublication = {yes},
journal = {Bio-protocol},
note = {CRIS-Team WoS Importer:2019-02-21},
peerreviewed = {Yes},
title = {{Murine} {Hair} {Follicle} {Derived} {Stem} {Cell} {Transplantation} onto the {Cornea} {Using} a {Fibrin} {Carrier}},
volume = {8},
year = {2018}
}
@article{faucris.119878044,
abstract = {To describe myofibroblastic metaplasia of corneal endothelial cells in 2 cases with impaired visual function despite complete graft adherence after Descemet membrane endothelial keratoplasty (DMEK).Interventional case series.In 2 of 90 consecutive DMEK surgeries, the cornea failed to clear up to 6 months postoperatively despite complete graft attachment. After secondary penetrating keratoplasty, both corneal buttons were examined using histopathologic analysis and transmission electron microscopy.Light microscopy revealed distinct corneal endothelial cell attenuation with the presence of an abnormal posterior collagenous layer in both cases. Most of the remaining endothelial cells had an elongated fibroblast-like appearance with immunopositivity for ?-smooth muscle actin indicative of myofibroblast metaplasia. Transmission electron microscopy showed a slightly thickened Descemet membrane with an abnormal posterior fibrillar collagenous layer and a myofibroblast-like transformation of the remaining endothelial cells. Descemet membrane grafts closely adjoined the collagen lamellae of the host corneal stroma similar to the Descemet membrane-stroma interface of a normal cornea.Myofibroblastic metaplasia of attenuated corneal endothelial cells with formation of an abnormal posterior collagenous layer may contribute to an impaired visual function despite complete graft adherence after Descemet membrane endothelial keratoplasty (DMEK).},
author = {Heindl, Ludwig M. and Schlötzer-Schrehardt, Ursula and Cursiefen, Claus and Bachmann, Bjoern O. and Hofmann-Rummelt, Carmen and Kruse, Friedrich},
doi = {10.1016/j.ajo.2010.11.032},
faupublication = {yes},
journal = {American Journal of Ophthalmology},
note = {EVALuna2:21011},
pages = {1019-1023.e2},
peerreviewed = {Yes},
title = {{Myofibroblast} metaplasia after descemet membrane endothelial keratoplasty},
volume = {151},
year = {2011}
}
@article{faucris.120523304,
abstract = {No standardized biomaterial exists for the surgical treatment of persistent corneal erosions and ulcerations. We analyzed the suitability and biocompatibility of defined noncross-linked and UV/riboflavin cross-linked equine type I collagen membranes for the reconstruction of the corneal surface. Isolated human oral mucosa epithelial cells, a cell type in clinical use for the treatment of ocular surface diseases, were subcultivated on both types of membranes and examined concerning cell adhesion, proliferation, and differentiation. Biocompatibility was evaluated following superficial and intrastromal corneal transplantation in New Zealand white rabbits. In cell cultures all collagen membranes supported adhesion of oral mucosa epithelial cells leading to the formation of multilayered epithelial cell sheets. After intrastromal corneal implantation clinical signs of degradation were seen in all variants of collagen membranes, which was fastest in noncross-linked variants. The histological and ultrastructural level invasion of keratocytes and production of new collagen fibers inside the collagen membranes could be detected in noncross-linked variants. After superficial corneal implantation covering of the membranes by corneal epithelium over time was visible. Ultrastructural analysis showed a slower rate of degradation and less invading keratocytes in cross-linked variants compared with noncross-linked collagen membranes. Cross-linked and noncross-linked variants of the collagen membrane proofed to be suitable to serve as a carrier for epithelial stem cells in vitro and showed a high biocompatibility in vivo. These results indicate that the tested collagen membranes might be suitable for the reconstruction of the corneal surface in patients with nonhealing ulcerations. Whether membranes with faster or slower degradation properties are preferable for the treatment of persistent corneal ulcerations might depend on the underlying corneal pathology and the degree of concomitant inflammation.},
author = {Petsch, Corinna and Schlötzer-Schrehardt, Ursula and Meyer-Blazejewska, Ewa and Frey, Markus and Kruse, Friedrich and Bachmann, Björn},
doi = {10.1089/ten.TEA.2013.0552},
faupublication = {yes},
journal = {Tissue Engineering: Parts A, B, and C},
note = {EVALuna2:21203},
pages = {2378-89},
peerreviewed = {Yes},
title = {{Novel} collagen membranes for the reconstruction of the corneal surface},
volume = {20},
year = {2014}
}
@inproceedings{faucris.207809781,
author = {Zenkel, Matthias and Berner, Daniel and Hoja, Ursula and Pasutto, Francesca and Kruse, Friedrich and Schlötzer-Schrehardt, Ursula},
faupublication = {yes},
note = {EVALuna2:34739},
peerreviewed = {Yes},
title = {{Nutritional} and hormonal regulation of lysyl oxidase-like 1 and elastic proteins involved in pseudoexfoliation syndrome/glaucoma},
volume = {59},
year = {2018}
}
@article{faucris.119388324,
abstract = {The expansion of donor derived corneal endothelial cells is a promising approach for regenerative therapies in corneal diseases. To achieve the best GMP standard the entire cultivation process should be devoid of non-human components. However, so far there is no suitable xeno-free protocol for clinical applications.We therefore introduce a processed variant of a platelet lysate for the use in corneal cell and tissue culture based on a GMP-grade thrombocyte concentrate. This processed human platelet lysate (phPL), free of any animal components and of anti-coagulants like heparin with a physiological ionic composition, was used to cultivate corneal endothelial cells (EC) in vitro and ex vivo in comparison to standard cultivation with FCS. Human donor corneas were cut in quarters while two quarters of each cornea were incubated with the respective medium supplement. Three fields of view per quarter were taken into account for the analysis.Evaluation of phPL as a medium supplement in cell culture of immortalized EC showed a superior viability compared to fetal calf serum (FCS) control with reduced cell proliferation. Furthermore, the viability during the expansion of primary cells is significantly (3fold+-0.5) increased with phPL compared to FCS standard medium. Quartering donor corneas was traumatic for the endothelium and therefore resulted in increased EC loss. Interestingly, however, cultivation of the quartered pieces for two weeks in 0.1mg/mL pHPL in Biochrome I showed a 21 (+-10) % EC loss compared to 67 (+-12) % EC loss when cultivated in 2% FCS in Biochrome I.The cell culture protocol with pHPL as FCS replacement seems to be superior to the standard FCS protocols with respect to EC survival. It offers a xeno-free and physiological environment for corneal endothelial cells. This alternative cultivation protocol could facilitate the use of EC for human corneal cell therapy.},
author = {Thieme, Daniel and Reuland, Lynn and Lindl, Toni and Kruse, Friedrich and Fuchsluger, Thomas},
doi = {10.1002/term.2574},
faupublication = {yes},
journal = {Journal of Tissue Engineering and Regenerative Medicine},
note = {EVALuna2:21343},
peerreviewed = {Yes},
title = {{Optimized} human platelet lysate as novel basis for a serum-, xeno- and additive-free corneal endothelial cell and tissue culture},
year = {2017}
}
@article{faucris.107830624,
abstract = {To review recent advances in posterior lamellar keratoplasty and to describe strategies that enhance the outcome of Descemet's membrane endothelial keratoplasty (DMEK) and should lead to a more widespread use of this technique.DMEK offers significant advantages over Descemet's stripping automated endothelial keratoplasty (DSAEK) such as less immune reaction and better visual acuity because of less higher order aberrations. Donor selection should exclude donors under 50 years because of tissue elasticity; several advanced techniques now allow donor preparation from both cold and organ-cultured tissue in about 99% minimizing the risk of graft loss. Oversizing the area of Descemet's stripping in relationship to graft size enhances graft attachment and use of a standardized approach for graft delivery. Air bubble-driven nontouch unfolding techniques and, possibly, gas tamponade in the anterior chamber further enhance graft attachment and reduce surgery-induced endothelial cell loss. Graft orientation is made earlier by marking, slit beam and optical coherence tomography. Novel understanding of the functional anatomy of Descemet's membrane as well as migration of endothelial cells will allow to further refine DMEK and improve its outcome.Although the superiority of DMEK over Descemet's stripping automated endothelial keratoplasty in terms of safety and functionality had been further elucidated, remarkable progress has been made in the recent past regarding tissue preparation, insertion and intraoperative manipulation that will foster the more widespread use of DMEK among corneal surgeons.},
author = {Kruse, Friedrich and Schlötzer-Schrehardt, Ursula and Tourtas, Theofilos},
doi = {10.1097/ICU.0000000000000072},
faupublication = {yes},
journal = {Current Opinion in Ophthalmology},
note = {EVALuna2:21204},
pages = {325-34},
peerreviewed = {Yes},
title = {{Optimizing} outcomes with {Descemet}'s membrane endothelial keratoplasty},
volume = {25},
year = {2014}
}
@article{faucris.123881824,
abstract = {To determine whether clinical performance is negatively affected by prestripping Descemet membrane endothelial keratoplasty (DMEK) grafts from organ-cultured corneas.We reviewed clinical records of all patients who underwent DMEK surgery for Fuchs endothelial dystrophy between 28 October 2014 and 11 August 2015. Grafts had been prepared from organ-cultured corneoscleral buttons 24 hours prior to surgery or during surgery. We included only patients for which at least one follow-up examination was available at a minimum of 2 months postoperatively.best-corrected visual acuity (BCVA), central corneal thickness, endothelial cell count and rebubbling rates.Data given are mean±SD. No statistically significant differences were recorded at baseline between the partially stripped group (n=65) and the control group (n=72) with regard to donor age (70±9 vs 69±8 years; p=0.49), donor cornea endothelial cell density (2586±604 vs 2522±186 cells/mm(2); p=0.6), BCVA (before DMEK: 0.77±0.5 vs 0.63±0.3 logMAR; p=0.27; before triple-DMEK: 0.56±0.2 vs 0.52±0.2 logMAR; p=0.33) or central corneal thickness (621±72 vs 607±53 ?m; p=0.49). Mean follow-up was 149±83 versus 148±77 days; p=0.79. No statistically significant differences were observed between the two groups postoperatively with regard to BCVA (after DMEK: 0.25±0.2 vs 0.21±0.2 logMAR; p=0.59; after triple-DMEK: 0.22±0.2 vs 0.2±0.1 logMAR; p=0.98), central corneal thickness (502±42 vs 508±41 ?m; p=0.47), endothelial cell count (1537±245 vs 1551±287 cells/mm(2); p=0.65) and number of graft detachments requiring rebubbling (4.6% vs 9.7%; p=0.33).We found no evidence that the use of prestripped DMEK grafts is inferior to same-day preparation in organ-cultured corneas within the given follow-up.},
author = {Menzel-Severing, Johannes and Kruse, Friedrich and Tourtas, Theofilos},
doi = {10.1136/bjophthalmol-2016-309550},
faupublication = {yes},
journal = {British Journal of Ophthalmology},
note = {EVALuna2:21351},
pages = {1124-1127},
peerreviewed = {Yes},
title = {{Organ}-cultured, prestripped donor tissue for {DMEK} surgery: clinical outcomes},
volume = {101},
year = {2017}
}
@article{faucris.234244884,
author = {Deng, Sophie X. and Borderie, Vincent and Chan, Clara C. and Dana, Reza and Figueiredo, Francisco C. and Gomes, Jose A. P. and Pellegrini, Graziella and Shimmura, Shigeto and Kruse, Friedrich},
doi = {10.1097/ICO.0000000000002145},
faupublication = {yes},
journal = {Cornea},
note = {CRIS-Team WoS Importer:2020-02-14},
pages = {E56-E57},
peerreviewed = {No},
title = {{Other} {Causes} of {Limbal} {Stem} {Cell} {Deficiency} {Reply}},
volume = {38},
year = {2019}
}
@article{faucris.120894224,
abstract = {Palate Lung Nasal Clone (PLUNC) is a hydrophobic protein belonging to the family of surfactant proteins that is involved in fluid balance regulation of the lung. Moreover, it is known to directly act against gram-negative bacteria. The purpose of this study was to investigate the possible expression and antimicrobial role of PLUNC at the healthy ocular surface and in tears of patients suffering from dry eye disease (DED).Bioinformatics and biochemical and immunologic methods were combined to elucidate the structure and function of PLUNC at the ocular surface. Tissue-specific localization was performed by using immunohistochemistry. The PLUNC levels in tear samples from non-Sjögren's DED patients with moderate dry eye suffering either from hyperevaporation or tear deficiency were analyzed by ELISA and compared with tears from healthy volunteers.Palate Lung Nasal Clone is expressed under healthy conditions at the ocular surface and secreted into the tear film. Protein modeling studies and molecular dynamics simulations performed indicated surface activity of PLUNC. In vitro experiments revealed that proinflammatory cytokines and bacterial supernatants have only a slight effect on the expression of PLUNC in HCE and HCjE cell lines. In tears from DED patients, the PLUNC concentration is significantly increased (7-fold in evaporative dry eye tears and 17-fold in tears from patients with tear deficiency) compared with healthy subjects.The results show that PLUNC is a protein of the tear film and suggest that it plays a role in fluid balance and surface tension regulation at the ocular surface.},
author = {Schicht, Martin and Rausch, Felix and Beron, Martin and Jacobi, Christina and Garreis, Fabian and Hartjen, Nadine and Beileke, Stephanie and Kruse, Friedrich and Bräuer, Lars and Paulsen, Friedrich},
doi = {10.1167/iovs.15-17560},
faupublication = {yes},
journal = {Investigative Ophthalmology & Visual Science},
note = {EVALuna2:4442},
pages = {7312-23},
peerreviewed = {Yes},
title = {{Palate} {Lung} {Nasal} {Clone} ({PLUNC}), a {Novel} {Protein} of the {Tear} {Film}: {Three}-{Dimensional} {Structure}, {Immune} {Activation}, and {Involvement} in {Dry} {Eye} {Disease} ({DED})},
volume = {56},
year = {2015}
}
@article{faucris.119874084,
abstract = {Purpose: To determine the elastic fiber content and ultrastructure as well as the expression of elastin-degrading enzymes in biopsy specimens from patients with involutional ectropion and entropion. Materials and Methods: Twenty consecutive patients with involutional ectropion (group 1) and twenty consecutive patients with entropion (group 2) were matched with twenty control patients (basal cell carcinoma) regarding age and gender. Full-thickness eyelid resections performed in study and control patients were examined by light and transmission electron microscopy, computer-assisted measurements, and immunohistochemistry using antibodies against matrix metalloproteinase (MMP)-2, MMP- 7, and MMP-9. The Kruskal-Wallis test and the Pearson chi-square test were performed. Results: Histopathologic analysis of the surgical specimens from patients with involutional ectropion and entropion showed a significant loss of elastic fibers in the eyelid skin, the pretarsal orbicularis oculi muscle, the perimeibomian tarsal stroma, and the intermeibomian tarsal stroma (P < 0.001). Residual elastic fibers revealed an abnormal ultrastructure. Immunohistochemistry demonstrated a significant overexpression of MMP- 2, MMP-7, and MMP-9 in the eyelid skin, the pretarsal orbicularis oculi muscle, the perimeibomian tarsal stroma, the intermeibomian tarsal stroma, and the conjunctiva in groups 1 and 2 compared to controls (P < 0.001). Conclusions: The present findings indicate that upregulation of elastolytic enzymes contributes to elastic fibre degradation in patients with involutional ectropion and entropion.},
author = {Damasceno, Renato W. and Heindl, Ludwig M. and Hofmann-Rummelt, Carmen and Belfort, Rubens and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich and Holbach, Leonard M.},
doi = {10.3109/01676830.2011.569049},
faupublication = {yes},
journal = {Orbit},
note = {EVALuna2:21015},
pages = {132-9},
peerreviewed = {Yes},
title = {{Pathogenesis} of involutional ectropion and entropion: the involvement of matrix metalloproteinases in elastic fiber degradation},
volume = {30},
year = {2011}
}
@article{faucris.123539064,
abstract = {Conjunctival melanoma is a rare but potentially fatal disease. The 10-year melanoma mortality can be up to 30 %, recurrence rates after treatment up to 50 % and the overall incidence of metastasis is 26 %. Improved treatment options are needed to increase the tumor-free survival of affected patients.The aim of the study was to perform clinical and pathological staging using the TNM classification and to correlate the results with treatment modalities and recurrence rates.The study included a case series of 80 eyes from 80 patients (42 females and 38 males, age 28-90 years) with histopathologically proven conjunctival melanoma studied by reviewing medical records, pathology reports and color photographs. The main evaluated characteristics were demographic information, tumor size, thickness, pathological diagnosis, BRAF mutation testing, clinical and pathological staging, recurrence, metastasis and duration of follow-up (mean 48 months).The lesions predominantly involved the bulbar conjunctiva (60 %) and other sites that were less often involved were the palpebral conjunctiva (23 %), conjunctival fornix (22 %) and lacrimal caruncle (15 %). Of the tumors 36 % were TNM classified as pTis, 34 % as pT1, 20 % as pT2 (palpebral, fornix and caruncle) and 10 % as pT3. Local recurrences were noted in 36 % of the patients (18 % Tis, 26 % T1, 32 % T2 and 70 % T3) and regional and distant metastasis in 20 % of the patients (0 % Tis, 10 % T1, 15 % T2 and 60 % T3).In this study increasing T stages were more often associated with recurrences and metastasis. Future studies correlating the TNM staging with histopathological and genetic predictors may help to improve the management of patients with conjunctival melanoma.},
author = {Berta-Antalics, A. I. and Kruse, Friedrich and Holbach, L.},
doi = {10.1007/s00347-015-0148-x},
faupublication = {yes},
journal = {Ophthalmologe},
note = {EVALuna2:21241},
pages = {892-8},
peerreviewed = {Yes},
title = {{Pathology} and prognostic factors of conjunctival melanoma},
volume = {112},
year = {2015}
}
@article{faucris.316358397,
abstract = {Purpose: Estimating glaucoma suspects’ risk for visual field defects helps to avoid under- and over-treatment. In this retrospective, longitudinal cohort study with a very long follow-up, we studied whether pattern electroretinograms (PERG) amplitudes and blue-on-yellow visual evoked potential (BY-VEP) latencies can predict visual field defects. Methods: Participants of the Erlangen Glaucoma Study were examined with PERG and BY-VEP between 9/1991 and 8/2001. Stimuli were created using an optical bench with Maxwellian view and consisted of vertical gratings (0,88 cpd) in a 32° field for both PERG and BY-VEP. Patients were treated according to clinical standards and performed standard automated perimetry (SAP) annually. Retrospectively, patients with normal SAP at baseline were selected. Primary endpoint was conversion to perimetric glaucoma. Predictive value was modeled using Kaplan–Meier analyses and a multivariate cox proportional hazards model with the continuous variables PERG amplitude, BY-VEP peak time and SAP square-root of loss variance (sLV) after stratification for Jonas classification of the optic discs. Results: Of 412 patients (288: Jonas 0, 103: I, and 21: II; baseline age: 20–60 years), 65 converted to perimetric glaucoma during follow-up (0.5–23.3 years; median 5.5 years). Optic disc classification was a strong risk factor for conversion (log rank p < 0.0001), and patients with more advanced changes progressed earlier. In the multivariate analysis (log rank p = 0.005), only PERG amplitude remained an independent risk factor after stratification for optic disc morphology (p = 0.021), with a ~ 30% higher risk per μV amplitude decrease. Conclusions: PERG helps to estimate glaucoma suspects’ risk for visual field defects.},
author = {Huchzermeyer, Cord and Lämmer, Robert and Mardin, Christian Y. and Kruse, Friedrich and Kremers, Jan and Horn, Folkert},
doi = {10.1007/s00417-023-06364-y},
faupublication = {yes},
journal = {Graefes Archive For Clinical and Experimental Ophthalmology},
keywords = {Electroretinogram; Glaucoma; Perimetry; Survival analysis; Visually evoked potentials},
note = {CRIS-Team Scopus Importer:2024-01-12},
peerreviewed = {Yes},
title = {{Pattern} electroretinogram, blue-yellow visual evoked potentials and the risk of developing visual field defects in glaucoma suspects: a longitudinal “survival” analysis with a very long follow-up},
year = {2024}
}
@article{faucris.120039524,
abstract = {To describe the clinical results of Pentacam-based big bubble deep anterior lamellar keratoplasty (DALK) to achieve an intended 90% depth of initial lamellar trephination.Fifty consecutive eyes of 50 patients with keratoconus, keratoglobus, and anterior stromal scars were included. DALK was performed with the big bubble technique using a 90% intended depth for initial lamellar trephination based on preoperative pachymetry by Pentacam. Main outcome measures were success of surgery, best spectacle-corrected visual acuity, endothelial cell count, refractive astigmatism at 12-month follow-up, and rate of intra- and postoperative complications.In 84% of the patients (n = 42), Pentacam-based big bubble DALK could be performed successfully. Successful big bubble formation could be achieved in 80% of the patients (n = 34). In case of macroperforation (n = 8), surgery was converted to standard penetrating keratoplasty representing a conversion rate of 16%. Intraoperative microperforation (n = 5) could be handled by an intracameral air injection at the end of operation with successful completion of the lamellar procedure. No allograft rejection was observed. Best spectacle-corrected visual acuity improved from 20/125 ± 20/160 preoperatively to 20/40 ± 20/80 at 12-month follow-up. Endothelial cell count was 2102 ± 318 cells per square millimeter preoperatively and 1735 ± 420 cells per square millimeter at 12-month follow-up. Refractive astigmatism was 7.09 ± 3.13 diopters preoperatively and decreased to 4.13 ± 2.41 diopters.Pentacam-based big bubble DALK using a 90% intended depth of initial lamellar trephination seems to be a safe and effective procedure for anterior corneal stromal disorders such as keratoconus. We suggest that Pentacam-based depth assessment allows for reliably deep initial preparation and may allow more successful bubble formation in DALK surgery.},
author = {Riss, Stephan and Heindl, Ludwig M. and Bachmann, Bjoern O. and Kruse, Friedrich and Cursiefen, Claus},
doi = {10.1097/ICO.0b013e31823f8c85},
faupublication = {yes},
journal = {Cornea},
note = {EVALuna2:21074},
pages = {627-32},
peerreviewed = {Yes},
title = {{Pentacam}-based big bubble deep anterior lamellar keratoplasty in patients with keratoconus},
volume = {31},
year = {2012}
}
@article{faucris.118001224,
abstract = {The purpose of this study was to analyze if anterior chamber parameters are risk factors for the development of pigment dispersion syndrome (PDS) and/or for the conversion to pigmentary glaucoma (PG).This study included a total of 63 eyes from 35 patients with PDS and PG and 65 eyes from 49 unaffected volunteers as the control group. The following parameters were measured by slit lamp optical coherence tomography (SL-OCT): anterior chamber volume (ACV) and depth (ACD), angle opening distance (AOD) and the trabecular iris space area (TISA) at 500 µm and 750 ?m from the scleral spur. Comparisons between the following groups were performed: between the PDS/PG and the control group, between PDS and PG and between male and female patients.The results of ACV, ACD, AOD and TISA were significantly higher in PDS/PG patients when compared to the control group. There were no significant differences between PDS and PG. The gender-specific comparison also showed no significant differences.Significantly higher anterior chamber parameters are a possible risk factor for development of PDS; however, a higher risk of conversion to PG does not seem to correlate with increased anterior chamber parameters. The parameters of the anterior chamber are apparently not associated with the male predominance of PDS and PG.},
author = {Birner, Barbara and Tourtas, T. and Wessel, J. M. and Jünemann, Anselm and Mardin, Christian Y. and Kruse, Friedrich and Lämmer, Robert},
doi = {10.1007/s00347-013-2943-6},
faupublication = {yes},
journal = {Ophthalmologe},
note = {EVALuna2:21131},
pages = {638-43},
peerreviewed = {Yes},
title = {{Pigment} dispersion syndrome and pigmentary glaucoma. {Morphometric} analysis of the anterior chamber segment with {SL}-{OCT}},
volume = {111},
year = {2014}
}
@article{faucris.286397898,
abstract = {Post-COVID-19 syndrome (PCS) is characterized by persisting sequelae after infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). PCS can affect patients with all COVID-19 disease severities. As previous studies have revealed impaired blood flow as a provoking factor triggering PCS, it was the aim of the present study to investigate the potential association between self-reported chronic fatigue and retinal microcirculation in patients with PCS, potentially indicating an objective biomarker. A prospective study was performed, including 201 subjects: 173 patients with PCS and 28 controls. Retinal microcirculation was visualized by OCT angiography (OCT-A) and quantified using the Erlangen-Angio-Tool as macula and peripapillary vessel density (VD). Chronic fatigue (CF) was assessed according to the variables of Bell’s score, age and gender. VDs in the superficial vascular plexus (SVP), intermediate capillary plexus (ICP) and deep capillary plexus (DCP) were analyzed, considering the repetitions (12 times). Seropositivity for autoantibodies targeting G protein-coupled receptors (GPCR-AAbs) was determined by an established cardiomyocyte bioassay. Taking account of the repetitions, a mixed model was performed to detect possible differences in the least square means between the different groups included in the analysis. An age effect in relation to VD was observed between patients and controls (p < 0.0001). Gender analysis showed that women with PCS showed lower VD levels in the SVP compared to male patients (p = 0.0015). The PCS patients showed significantly lower VDs in the ICP as compared to the controls (p = 0.0001 (CI: 0.32; 1)). Moreover, considering PCS patients, the mixed model revealed a significant difference between those with chronic fatigue (CF) and those without CF with respect to VDs in the SVP (p = 0.0033 (CI: −4.5; −0.92)). The model included variables of age, gender and Bell’s score, representing a subjective marker for CF. Consequently, retinal microcirculation might serve as an objective biomarker in subjectively reported chronic fatigue in patients with PCS.},
author = {Schlick, Sarah and Lucio, Marianna and Wallukat, Gerd and Bartsch, Alexander and Skornia, Adam and Hoffmanns, Jakob and Szewczykowski, Charlotte and Schröder, Thora and Raith, Franziska and Rogge, Lennart and Heltmann, Felix and Moritz, Michael and Beitlich, Lorenz and Schottenhamml, Julia and Herrmann, Martin and Harrer, Thomas and Ganslmayer, Marion and Kruse, Friedrich and Lämmer, Robert and Mardin, Christian and Hohberger, Bettina},
doi = {10.3390/ijms232213683},
faupublication = {yes},
journal = {International Journal of Molecular Sciences},
keywords = {chronic fatigue; chronic fatigue syndrome; COVID-19; functional GPCR autoantibodies; long COVID syndrome; OCT angiography; post-COVID-19 syndrome; retinal microcirculation},
note = {CRIS-Team Scopus Importer:2022-12-09},
peerreviewed = {Yes},
title = {{Post}-{COVID}-19 {Syndrome}: {Retinal} {Microcirculation} as a {Potential} {Marker} for {Chronic} {Fatigue}},
volume = {23},
year = {2022}
}
@article{faucris.120811064,
abstract = {Alternative mRNA splicing coupled to nonsense-mediated decay (NMD) is a common mRNA surveillance pathway also known to dynamically modulate gene expression in response to cellular stress. Here, we investigated the involvement of this pathway in the regulation of lysyl oxidase-like 1 (LOXL1) expression in response to pseudoexfoliation (PEX)-associated pathophysiologic factors.Transcript levels of LOXL1 isoforms were determined in ocular tissues obtained from donor eyes without and with PEX syndrome. Pseudoexfoliation-relevant cell types, including human Tenon's capsule fibroblasts (hTCF) and trabecular meshwork cells (hTMC), were exposed to puromycin, caffeine, TGF-?1, homocysteine, IL-6, retinoic acid, UV-B radiation, oxidative stress, and mechanical stress for up to 48 hours. Western blot analysis was carried out using antibodies against LOXL1, (phosphorylated-) eukaryotic initiation factor 2-? (eIF2-?), and regulator of nonsense transcripts 2 (UPF2). RNA interference was used to knockdown UPF1-3 and Serine/threonine-protein kinase (SMG1).Constitutive expression of wild-type LOXL1 and alternatively spliced LOXL1-a transcripts was detected in all ocular tissues showing highest levels in trabecular meshwork and differential expression between PEX and control specimens. LOXL1-a transcripts were upregulated in hTCF and hTMC by NMD inhibitors puromycin and caffeine (>=6-fold; P < 0.01) or after knockdown of NMD core factors (>=2-fold; P < 0.05), whereas mRNA and protein levels of LOXL1 were reduced (<=0.8 fold; P < 0.05). Exposure of cells to various PEX-associated (stress) factors, including TGF-?1, UV-B light, oxidative stress, mechanical stress, and retinoic acid enhanced LOXL1-a transcript levels (>=1.5-fold; P < 0.05), while partially downregulating LOXL1 levels (<=0.7-fold; P < 0.05). Stress-induced inhibition of NMD was dependent on phosphorylation of eIF2?.These findings provide evidence for a functional role of alternative splicing coupled to NMD in the posttranscriptional regulation of LOXL1 gene expression and suggest this mechanism to represent a dynamic mode of adapting LOXL1 expression to PEX-associated environmental and nutritional cues.},
author = {Berner, Daniel and Zenkel, Matthias and Pasutto, Francesca and Hoja, Ursula and Liravi, Panah and Gusek-Schneider, Gabriele C. and Kruse, Friedrich and Schödel, Johannes and Reis, André and Schlötzer-Schrehardt, Ursula},
doi = {10.1167/iovs.17-22963},
faupublication = {yes},
journal = {Investigative Ophthalmology & Visual Science},
note = {EVALuna2:9393},
pages = {5930-5940},
peerreviewed = {Yes},
title = {{Posttranscriptional} {Regulation} of {LOXL1} {Expression} {Via} {Alternative} {Splicing} and {Nonsense}-{Mediated} {mRNA} {Decay} as an {Adaptive} {Stress} {Response}},
volume = {58},
year = {2017}
}
@inproceedings{faucris.228587004,
address = {ROCKVILLE},
author = {Kruse, Friedrich and Tourtas, Theofilos and Zenkel, Matthias and Kinoshita, Shigeru and Schlötzer-Schrehardt, Ursula},
booktitle = {INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE},
date = {2019-04-28/2019-05-02},
faupublication = {yes},
note = {CRIS-Team WoS Importer:2019-11-01},
peerreviewed = {unknown},
publisher = {ASSOC RESEARCH VISION OPHTHALMOLOGY INC},
title = {{Potential} functional restoration of corneal endothelial cells in {Fuchs} corneal dystrophy by {ROCK} inhibitor ({Ripasudil})},
venue = {Vancouver},
year = {2019}
}
@article{faucris.249331449,
abstract = {Purpose: Rho-associated kinase (ROCK) inhibitors have been successfully used as a rescue strategy in eyes that failed to clear after descemetorhexis without endothelial graft for treatment of Fuchs endothelial corneal dystrophy (FECD). The functional mechanisms by which ROCK inhibitors modulate corneal endothelial cell regeneration in FECD patients have, however, not been clarified. Here, we analyzed the effect of the ROCK inhibitor ripasudil on corneal endothelial cells of FECD patients and normal donors using ex vivo tissue and in vitro cellular models. Design: Experimental study: laboratory investigation. Methods: This institutional study used endothelial cell–Descemet membrane lamellae from FECD patients (n = 450) undergoing Descemet membrane endothelial keratoplasty (FECD ex vivo model), normal research-grade donor corneas (n = 30) after scraping off central endothelial cells (ex vivo wound healing model), normal donor corneas (n = 20) without endothelial injury, and immortalized cell lines (n = 3) generated from FECD patients (FECD in vitro model). Descemet membrane lamellae were dissected into halves and incubated for 24-72 hours in storage medium with or without a single dose of 30 μM ripasudil. The effects of ripasudil on expression of genes and proteins related to endothelial cell proliferation, migration, functionality, and endothelial-to-mesenchymal transition were analyzed and complemented by functional assays on FECD cell lines. Results: A single dose of ripasudil induced significant upregulation of genes and proteins related to cell cycle progression, cell-matrix adhesion and migration, as well as endothelial barrier and pump function up to 72 hours, whereas classical markers of endothelial-to-mesenchymal transition were downregulated in both FECD and normal specimens compared to unstimulated controls ex vivo. In addition to stimulation of proliferation and migration, ripasudil-induced changes in expression of functional signature genes could be also verified in FECD cell lines in vitro. Conclusions: These data support the concept that inhibition of ROCK signaling represents a potent tool in regenerative therapies in FECD patients through reactivation of cell proliferation and migration as well as restoration of endothelial pump and barrier function without inducing adverse phenotypic changes.},
author = {Schlötzer-Schrehardt, Ursula and Zenkel, Matthias and Strunz, Maria and Gießl, Andreas and Schondorf, Hannah and da Silva, Heather and Schmidt, Gregory A. and Greiner, Mark A. and Okumura, Naoki and Koizumi, Noriko and Kinoshita, Shigeru and Tourtas, Theofilos and Kruse, Friedrich},
doi = {10.1016/j.ajo.2020.12.006},
faupublication = {yes},
journal = {American Journal of Ophthalmology},
note = {CRIS-Team Scopus Importer:2021-02-12},
pages = {185-199},
peerreviewed = {Yes},
title = {{Potential} {Functional} {Restoration} of {Corneal} {Endothelial} {Cells} in {Fuchs} {Endothelial} {Corneal} {Dystrophy} by {ROCK} {Inhibitor} ({Ripasudil})},
volume = {224},
year = {2021}
}
@article{faucris.208896456,
abstract = {PURPOSE: The aim of this study was to assess the repeatability and reproducibility (R&R) of Bruch membrane opening based on minimum rim width (BMO-MRW), minimum rim area (BMO-MRA) and peripapillary retinal nerve fiber layer thickness (RNFLT) with the Spectralis optical coherence tomography (Heidelberg Engineering) for normal and glaucoma subjects. Precise measurement of these parameters can support detection of structural glaucomatous damage and progression.
METHODS: This cross-sectional study included 16 healthy controls and 16 patients with glaucoma. One eye was randomly selected and included in this study. Subjects underwent 1 baseline and 3 follow-up measurements, using 3 different Spectralis optical coherence tomography devices in randomized order, each operated by a single operator. Outcome measures were global and sectorial averages of BMO-MRW and BMO-MRA, and of peripapillary RNFLT obtained from 12/14/16-degree circle scans. Coefficients of variation (COV) were calculated and a mixed-effects analysis of variance was performed to compare R&R between devices.
RESULTS: COVs of global and sectorial BMO-MRW measurement under repeatability conditions ranged from 0.51% to 1.7% (normal, 0.62% to 1.3%; glaucoma, 0.64% to 2.3%). Respective COVs under reproducibility conditions ranged from 0.89% to 1.9% (normal, 0.77% to 2.8%; glaucoma, 1.1% to 2.6%). COVs of global and sectorial RNFLT measurements under repeatability conditions ranged from 0.5% to 2.8%. Respective COVs under reproducibility conditions ranged from 1.6% to 3.5%.
CONCLUSIONS: For R&R, the COVs of measured parameters were by trend higher for glaucoma eyes compared with normal controls. The BMO-MRW measurement system has an excellent precision taking into account that major and minor corrections of segmentation have to be done by the examiner before evaluation.},
author = {Schrems-Hösl, Laura-Maria and Schrems, Wolfgang and Lämmer, Robert and Kruse, Friedrich and Mardin, Christian Y.},
doi = {10.1097/IJG.0000000000000875},
faupublication = {yes},
journal = {Journal of Glaucoma},
note = {EVALuna2:34751},
pages = {407-414},
peerreviewed = {Yes},
title = {{Precision} of {Optic} {Nerve} {Head} and {Retinal} {Nerve} {Fiber} {Layer} {Parameter} {Measurements} by {Spectral}-domain {Optical} {Coherence} {Tomography}},
volume = {27},
year = {2018}
}
@article{faucris.121927564,
abstract = {New methods are needed to compare peripapillary retinal nerve fiber layer thickness (pRNFLT) measurements taken from time-domain optical coherence tomography (TD-OCT) and spectral-domain OCT (SD-OCT).To compare the agreement of measured and predicted pRNFLT using different equations based on pRNFLT measurements obtained by TD-OCT and SD-OCT.Cross-sectional single-center study that took place at the Department of Ophthalmology, University of Erlangen-Nuremberg from November 16, 2005, to June 3, 2015, and included 138 eyes of control participants, 126 eyes of patients with ocular hypertension, 128 eyes of patients with preperimetric glaucoma, and 160 eyes of patients with perimetric glaucoma. All participants had standard clinical examinations to obtain TD-OCT (via Stratus OCT) and SD-OCT (via Spectralis OCT) measurements of pRNFLT. Two groups were matched for diagnostic subgroup, eye side, sex, and age. The TD-OCT measurements of the first group were used to predict the mean SD-OCT and 6-sector vertical-split pRNFLT measurements of the second group and vice versa. The agreement between the predicted pRNFLT calculations of conversion equations and measured pRNFLT of the second group was evaluated by intraclass correlation coefficients and Bland-Altman plots.Mean and sectoral pRNFLT measurements obtained by TD-OCT and SD-OCT as well as the agreement between measured and predicted pRNFLT.The agreement for all investigated equations to predict mean pRNFLT measurements with intraclass correlation coeffecients ranged from 0.937 to 0.939. Bland-Altman plots demonstrated systemic biases between -0.7 ?m and +1.1 ?m for measured and predicted mean pRNFLT measurements. The ratio method demonstrated an intraclass correlation coefficient of 0.969 for the temporal-inferior sector. The best color-code agreement between both OCT devices was achieved by the no conversion method, with ? = 0.731 (95% CI, 0.656-0.806) for the mean pRNFLT.These data suggest that the prediction of mean pRNFLT values by equations derived from TD-OCT and SD-OCT can be conducted with high levels of agreement. In individual cases and singular sectors, high prediction errors may occur. When longitudinal imaging data from both TD-OCT and SD-OCT are available, conversion equations may provide longitudinal comparability.},
author = {Schrems, Wolfgang and Schrems-Hösl, Laura-Maria and Bendschneider, Delia and Mardin, Christian Y. and Lämmer, Robert and Kruse, Friedrich and Horn, Folkert},
doi = {10.1001/jamaophthalmol.2015.2427},
faupublication = {yes},
journal = {JAMA Ophthalmology},
note = {EVALuna2:21226},
pages = {1135-43},
peerreviewed = {Yes},
title = {{Predicted} and {Measured} {Retinal} {Nerve} {Fiber} {Layer} {Thickness} {From} {Time}-{Domain} {Optical} {Coherence} {Tomography} {Compared} {With} {Spectral}-{Domain} {Optical} {Coherence} {Tomography}},
volume = {133},
year = {2015}
}
@article{faucris.208895993,
abstract = {PURPOSE: The purpose of this study was to compare the ability of scanning laser polarimetry (SLP) and spectral-domain optical coherence tomography (SD-OCT) to predict future visual field conversion of subjects with ocular hypertension and early glaucoma.
METHODS: All patients were recruited from the Erlangen glaucoma registry and examined using standard automated perimetry, 24-hour intraocular pressure profile, and optic disc photography. Peripapillary retinal nerve fiber layer thickness (RNFL) measurements were obtained by SLP (GDx-VCC) and SD-OCT (Spectralis OCT). Positive and negative predictive values (PPV, NPV) were calculated for morphologic parameters of SLP and SD-OCT. Kaplan-Meier survival curves were plotted and log-rank tests were performed to compare the survival distributions. Contingency tables and Venn-diagrams were calculated to compare the predictive ability.
RESULTS: The study included 207 patients-75 with ocular hypertension, 85 with early glaucoma, and 47 controls. Median follow-up was 4.5 years. A total of 29 patients (14.0%) developed visual field conversion during follow-up. SLP temporal-inferior RNFL [0.667; 95% confidence interval (CI), 0.281-0.935] and SD-OCT temporal-inferior RNFL (0.571; 95% CI, 0.317-0.802) achieved the highest PPV; nerve fiber indicator (0.923; 95% CI, 0.876-0.957) and SD-OCT mean (0.898; 95% CI, 0.847-0.937) achieved the highest NPV of all investigated parameters. The Kaplan-Meier curves confirmed significantly higher survival for subjects within normal limits of measurements of both devices (P<0.001). Venn diagrams tested with McNemar test statistics showed no significant difference for PPV (P=0.219) or NPV (P=0.678).
CONCLUSIONS: Both GDx-VCC and SD-OCT demonstrate comparable results in predicting future visual field conversion if taking typical scans for GDx-VCC. In addition, the likelihood ratios suggest that GDx-VCC's nerve fiber indicator<30 may be the most useful parameter to confirm future nonconversion. (http://www.ClinicalTrials.gov number, NTC00494923; Erlangen Glaucoma Registry).},
author = {Diekmann, Theresa and Schrems-Hösl, Laura-Maria and Mardin, Christian Y. and Lämmer, Robert and Horn, Folkert and Kruse, Friedrich and Schrems, Wolfgang},
doi = {10.1097/IJG.0000000000000833},
faupublication = {yes},
journal = {Journal of Glaucoma},
note = {EVALuna2:34215},
pages = {157-163},
peerreviewed = {Yes},
title = {{Predictive} {Factors} for {Visual} {Field} {Conversion}: {Comparison} of {Scanning} {Laser} {Polarimetry} and {Optical} {Coherence} {Tomography}},
volume = {27},
year = {2018}
}
@article{faucris.112678324,
abstract = {To investigate the presence and distribution of l-kynurenine aminotransferases immunoreactivity in human and animal lenses during cataract formation.Immunohistochemistry was conducted using polyclonal antibodies against KAT I, KAT II and KAT III on sections of 26 anterior capsules from patients undergoing surgical treatment of anterior subcapsular cataract (ASC) and 22 cataractous lenses from human eyes enucleated because of choroidal malignant melanoma. Additionally, the eyes of 11-month-old DBA/2J mice (6 eyes) were investigated (with KAT I and II). Ten clear human lenses and four BL6 mice lenses were used as controls. Spatial immunoreactivity patterns of enzymes were compared with Periodic Acid - Schiff (PAS)-stained sections.Immunohistochemical analysis revealed presence of KAT I, KAT II and KAT III in extracellular structures of all studied types of cataract in human eyes showing specific pattern of the stain. In cortical cataract, immunoreactivity was observed on cortical lens fibres. In nuclear cataract, KAT II revealed stronger and diffused staining than KAT I. Additionally, both KAT showed more pronounced staining at the edge of small clefts. In normal human lenses, KAT I, II and III, immunoreactivity was not observed. Presence of KAT I and KAT II in the intercellular substance of DBA/2J mice cataract was observed. In BL6 mice lenses without cataract, only weak KAT I and KAT II staining was observed.Presence of l-kynurenine aminotransferases in extracellular matrix (ECM) during human cataract formation suggests that products of l-kynurenine pathway might be involved in mechanisms of cataractogenesis.},
author = {Rejdak, Robert and Oleszczuk, Agnieszka and Rummelt, Carmen and Turski, Waldemar A. and Choragiewicz, Tomasz and Nowomiejska, Katarzyna and Ksiazek, Katarzyna and Thaler, Sebastian and Zarnowski, Tomasz and Okuno, Etsuo and Grieb, Pawel and Zrenner, Eberhart and Kruse, Friedrich and Junemann, Anselm G. M.},
doi = {10.1111/aos.12138},
faupublication = {yes},
journal = {Acta Ophthalmologica},
note = {EVALuna2:21117},
pages = {e450-5},
peerreviewed = {Yes},
title = {{Presence} and distribution of {L}-kynurenine aminotransferases immunoreactivity in human cataractous lenses},
volume = {91},
year = {2013}
}
@article{faucris.108640224,
abstract = {Corpora amylacea (CAm) are a hallmark of aging and neurodegeneration. The presence of kynurenine aminotransferases I and II (KAT I and II) in CAm in the human retina and optic nerve has been already shown. The present study aimed to examine kynurenine aminotransferase III (KAT III) immunoreactivity in CAm in the human retina and optic nerve.Polyclonal antibody against KAT III was used on sections of human eyes enucleated due to malignant uveal melanoma. PAS-stained sections of CAm were compared with KAT III stained ones.KAT III immunoreactivity was observed in CAm in the retina, prelaminar, laminar and retrolaminar region of the optic nerve with similar location to PAS-stained sections. The most intense staining was observed in the retrolaminar part of the optic nerve. KAT III immunoreactivity was also present in the cytoplasm of retinal ganglion cells.Expression of KAT III in CAm in the human retina and optic nerve indicates that this enzyme may be relevant in mechanisms of neurodegeneration leading to CAm formatio},
author = {Rejdak, Robert and Rummelt, Carmen and Zrenner, Eberhart and Grieb, Pawel and Rejdak, Konrad and Okuno, Etsuo and Thaler, Sebastian and Nowomiejska, Katarzyna and Kruse, Friedrich and Turski, Waldemar and Jünemann, Anselm},
faupublication = {yes},
journal = {Folia Neuropathologica},
note = {EVALuna2:21028},
pages = {132-7},
peerreviewed = {Yes},
title = {{Presence} of {L}-kynurenine aminotransferase {III} in retinal ganglion cells and corpora amylacea in the human retina and optic nerve},
volume = {49},
year = {2011}
}
@article{faucris.106656484,
abstract = {To describe a patient with interlamellar stromal keratitis induced by increased intraocular pressure (IOP) [Pressure-induced interlamellar stromal keratitis (PISK)] after laser in situ keratomileusis (LASIK) surgery.Case report and review of the literature.We report a case of interlamellar stromal keratitis induced by increased IOP after LASIK surgery. A 42-year-old man presented with persistent interface haze after uneventful LASIK. The patient described onset of decreased visual acuity after the first 2 postoperative weeks, failed to improve with high-dose topical steroid drops, and had significantly elevated IOP values up to 48 mm Hg. IOP was resistant to maximal topical antiglaucomatous therapy. The patient showed both improvement in visual acuity and decrease in interface haze after discontinuation of topical steroids and lowering of IOP by both topical and systemic treatment. Slit-lamp optical coherence tomography ruled out a fluid accumulation in the interface.PISK appears clinically almost identical to diffuse lamellar keratitis after LASIK. It is important to measure the IOP and be suspicious when a diffuse interface haze occurs after the first postoperative week, is resistant to or even exacerbates in response to an increase in topical steroid treatment, and is not associated with other causative events. Slit-lamp optical coherence tomography is a valuable tool that allows differentiation between space-occupying interface fluid collection and non-space-occupying interface fluid collection to avoid falsely low or normal IOP measurements in PISK.},
author = {Kruse, Friedrich and et al.},
author_hint = {Tourtas T, Kopsachilis N, Meiller R, Kruse FE, Cursiefen C.},
doi = {10.1097/ICO.0b013e3182032002},
faupublication = {yes},
journal = {Cornea},
note = {EVALuna2:21052},
pages = {920-3},
peerreviewed = {Yes},
support_note = {Author relations incomplete. You may find additional data in field 'author{\_}hint'},
title = {{Pressure}-induced interlamellar stromal keratitis after laser in situ keratomileusis},
volume = {30},
year = {2011}
}
@article{faucris.119652544,
abstract = {Pathologic processes in glaucoma include increased apoptosis, accumulation of extracellular material in the trabecular meshwork and optic nerve, condensations of the cytoskeleton and precocious cellular senescence. Oxidative stress was shown to generate these alterations in primary ocular cells. Fatty acids omega-3 and -6 are alleged to constitute a prophylaxis against these deleterious effects. Here, we tested actual preventive effects omega-3 and -6 against peroxide induced stress responses in primary human trabecular meshwork cells. Changes of mitochondrial activity, proliferation, heat shock proteins, extracellular matrix components, and inflammatory markers were evaluated. Alterations of the cytoskeleton were evaluated by phalloidin labeling. Here we report a repressive effect of omega-6 on metabolic activity and proliferation, which was not detected for omega-3. Both agents were able to prevent the anti-proliferative effect of H?O?, but only omega-3 prevented metabolic repression. Expression of heat shock protein 27 was unaltered by both fatty acids, whereas heat shock protein 90 was significantly induced by both. Omega-6 increased fibronectin and connective tissue growth factor synthesis, as well as the amount of secreted fibronectin. Omega-3, instead, induced plasminogen activator inhibitor 1 synthesis. H?O? further increased fibronectin production in omega-6 supplemented cells, which was not the case in omega-3 treated cells. H?O? stimulation of plasminogen activator inhibitor 1 and connective tissue growth factor was repressed by both fatty acids. Both fatty acids appeared to abolish H?O? mediated stimulation of nuclear factor ?B and IL-6, but not IL-1? and IL-8. H?O? induced formation of cross-linked actin networks and stress fibers, which was reduced by preemptive application of omega-3. Omega-6, in contrast, had no protective effect on that, and even seemed to promote condensation. Based on the observed side effects of omega-6, omega-3 appears to be the more beneficial fatty acid in respect of prophylactic intake for prevention of a glaucomatous disease.},
author = {Tourtas, Theofilos and Birke, Marco and Kruse, Friedrich and Welge-Lüssen, Ulrich-Christoph and Birke, Kerstin},
doi = {10.1371/journal.pone.0031340},
faupublication = {yes},
journal = {PLoS ONE},
note = {EVALuna2:21079},
pages = {e31340},
peerreviewed = {Yes},
title = {{Preventive} effects of omega-3 and omega-6 {Fatty} acids on peroxide mediated oxidative stress responses in primary human trabecular meshwork cells},
volume = {7},
year = {2012}
}
@article{faucris.119992664,
abstract = {To evaluate whether tumor-associated lymphangiogenesis contributes to prognosis of conjunctival malignant melanomas and to study its association with other tumor characteristics.Nonrandomized, retrospective case series.A total of 109 consecutive patients with primary conjunctival malignant melanoma.Proliferating lymphatic vessels were identified immunohistochemically using lymphatic vascular endothelial hyaluronan receptor-1 and podoplanin as specific lymphatic endothelial markers and Ki-67 as proliferation marker. Baseline tumor characteristics included tumor location, tumor thickness, tumor diameter, tumor origin, and tumor growth pattern. Kaplan-Meier and Cox regression analyses of the risk of local recurrence, lymphatic spread, distant metastasis, and melanoma-related death were performed.Intratumoral lymphatic vascular density and its association with tumor characteristics and recurrence-free, lymphatic spread-free, distant metastasis-free, and melanoma-specific survival.Intratumoral and peritumoral proliferating lymphatic vessels could be detected in all of the 109 conjunctival melanoma samples. High intratumoral lymphatic density was significantly associated with palpebral tumor location (P<0.001), greater tumor thickness (P<0.001), larger tumor diameter (P = 0.001), tumor origin de novo (P= 0.002), and nodular tumor growth pattern (P = 0.037). Patients with high intratumoral lymphatic density revealed significantly lower recurrence-free, lymphatic spread-free, distant metastasis-free, and melanoma-specific survival rates (P<0.001 for all). By multivariate Cox regression, factors predictive of local recurrence included palpebral tumor location (hazard ratio [HR] 2.66, P = 0.014), large tumor diameter (HR 5.48, P<0.001), and high intratumoral lymphatic density (HR 2.48, P = 0.043); factors predictive of lymphatic spread included palpebral tumor location (HR 4.13, P = 0.009), high tumor thickness (HR 12.17, P<0.001), and high intratumoral lymphatic density (HR 6.79, P = 0.019); factors predictive of distant metastasis included palpebral tumor location (HR 7.63, P<0.001), high tumor thickness (HR 8.60, P<0.001), large tumor diameter (HR 0.30, P = 0.029), and high intratumoral lymphatic density (HR 8.90, P = 0.047); and factors predictive of melanoma-related death included palpebral tumor location (HR 7.74, P<0.001), high tumor thickness (HR 10.88, P<0.001), large tumor diameter (HR 0.28, P = 0.018), and, with borderline significance, high intratumoral lymphatic density (HR 8.46, P = 0.052).Tumor-associated lymphangiogenesis seems to be associated with an increased risk of local recurrence, lymphatic spread, distant metastasis, and melanoma-related death in patients with conjunctival malignant melanomas.The author(s) have no proprietary or commercial interest in any materials discussed in this article.},
author = {Heindl, Ludwig M. and Hofmann-Rummelt, Carmen and Adler, Werner and Bosch, Jacobus J. and Holbach, Leonard M. and Naumann, Gottfried and Kruse, Friedrich and Cursiefen, Claus},
doi = {10.1016/j.ophtha.2011.05.025},
faupublication = {yes},
journal = {Ophthalmology},
note = {EVALuna2:5322},
pages = {2351-60},
peerreviewed = {Yes},
title = {{Prognostic} significance of tumor-associated lymphangiogenesis in malignant melanomas of the conjunctiva},
volume = {118},
year = {2011}
}
@article{faucris.119801264,
abstract = {The aim of this prospective, randomized, clinical, single-center study was to compare the safety and efficacy of 2 ocular surface lubricant eye drops: preservative-free hydroxypropyl (HP)-Guar (SYSTANE UD(®)) eye drops versus preservative-free Tamarindus indica seed polysaccharide (TSP) 1% (VISINE INTENSIV 1% EDO(®)) eye drops.Fifty-six eyes of 28 patients with moderate keratoconjunctivitis sicca (DEWS severity level 2) were enrolled in the trial. Patients were randomized for 2 treatment groups (SYSTANE UD eye drops vs. VISINE INTENSIV 1% EDO eye drops). The eye drops in both groups were applied 5 times per day for 3 months. Statistical analyses were performed using Statistica(TM) software (Mann-Whitney U-test and Wilcoxon test). P-Values<0.05 were considered significant.After 3 months of treatment the patients of both groups had subjective benefit in the relief of symptoms of dry eye disease evaluated by the Ocular Surface Disease Index (OSDI) questionnaire score. Patients treated with HP-Guar and TSP showed improvements in tear film stability measured by tear break-up time (TBUT), which are statistically significant in the HP-Guar group (P=0.02).The results of this clinical trial show improvements of symptoms and signs in patients with moderate dry eye after the consistent use of preservative-free HP-Guar and TSP lubricant eye drops. Both artificial tear formulations produce amelioration in tear film stability improving eye conditions and patient quality of life. HP-Guar seems to be slightly more effective in improving ocular surface protection by decreasing tear film evaporation.},
author = {Jacobi, Christina and Kruse, Friedrich and Cursiefen, Claus},
doi = {10.1089/jop.2012.0066},
faupublication = {yes},
journal = {Journal of Ocular Pharmacology and Therapeutics},
note = {EVALuna2:21112},
pages = {598-603},
peerreviewed = {Yes},
title = {{Prospective}, randomized, controlled comparison of {SYSTANE} {UD} eye drops versus {VISINE} {INTENSIV} 1% {EDO} eye drops for the treatment of moderate dry eye},
volume = {28},
year = {2012}
}
@inproceedings{faucris.265060296,
address = {ROCKVILLE},
author = {Nakagawa, Tatsuya and Okumura, Naoki and Hanada, Naoya and Padmanabhan, Prema and Elchuri, Sailaja and Chatterjee, Amit and Janakiraman, Narayanan and Sathe, Gajanan and Ghose, Vivek and Tourtas, Theofilos and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich and Koizumi, Noriko},
booktitle = {INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE},
faupublication = {yes},
note = {CRIS-Team WoS Importer:2021-10-15},
peerreviewed = {unknown},
publisher = {ASSOC RESEARCH VISION OPHTHALMOLOGY INC},
title = {{Proteomic} analysis of {TCF4} knock-out corneal endothelial cells derived from thepatients with {Fuchs} endothelial corneal dystrophy},
year = {2021}
}
@inproceedings{faucris.207809552,
author = {Berner, Daniel and Pasutto, Francesca and Hoja, Ursula and Zenkel, Matthias and Ozaki, Mineo and Williams, Susan and Ramsay, Michele and Carmichael, Trevor R. and Kruse, Friedrich and Aung, Tin and Khor, Chiea Chuen and Reis, André and Schlötzer-Schrehardt, Ursula},
faupublication = {yes},
note = {EVALuna2:34734},
peerreviewed = {Yes},
title = {{Pseudoexfoliation} associated protective variant, rs7173049, reveals a novel regulatory region downstream of {LOXL1}},
volume = {59},
year = {2018}
}
@article{faucris.119336184,
abstract = {Although lysyl oxidase-like 1 (LOXL1) is known as the principal genetic risk factor for pseudoexfoliation (PEX) syndrome, a major cause of glaucoma and cardiovascular complications, no functional variants have been identified to date. Here, we conduct a genome-wide association scan on 771 German PEX patients and 1,350 controls, followed by independent testing of associated variants in Italian and Japanese data sets. We focus on a 3.5-kb four-component polymorphic locus positioned spanning introns 1 and 2 of LOXL1 with enhancer-like chromatin features. We find that the rs11638944:C>G transversion exerts a cis-acting effect on the expression levels of LOXL1, mediated by differential binding of the transcription factor RXR? (retinoid X receptor alpha) and by modulating alternative splicing of LOXL1, eventually leading to reduced levels of LOXL1 mRNA in cells and tissues of risk allele carriers. These findings uncover a functional mechanism by which common noncoding variants influence LOXL1 expression.},
author = {Pasutto, Francesca and Zenkel, Matthias and Hoja, Ursula and Berner, Daniel and Uebe, Steffen and Ferrazzi, Fulvia and Schoedel, Johannes and Liravi, Panah and Ozaki, Mineo and Paoli, Daniela and Frezzotti, Paolo and Mizoguchi, Takanori and Nakano, Satoko and Kubota, Toshiaki and Manabe, Shinichi and Salvi, Erika and Manunta, Paolo and Cusi, Daniele and Gieger, Christian and Wichmann, Heinz-Erich and Aung, Tin and Khor, Chiea Chuen and Kruse, Friedrich and Reis, André and Schlötzer-Schrehardt, Ursula},
doi = {10.1038/ncomms15466},
faupublication = {yes},
journal = {Nature Communications},
note = {EVALuna2:9367},
pages = {15466},
peerreviewed = {Yes},
title = {{Pseudoexfoliation} syndrome-associated genetic variants affect transcription factor binding and alternative splicing of {LOXL1}},
volume = {8},
year = {2017}
}
@article{faucris.107833044,
abstract = {The aim of this study was to compare the efficacy and side effects of prednisolone acetate 1% versus fluorometholone 0.1% after Descemet membrane endothelial keratoplasty (DMEK).DMEK recipients used prednisolone acetate 1% for 1 month, and they were randomized to either prednisolone or fluorometholone for months 2 through 12. Dosing was 4 times daily in months 1 to 3, thrice daily in month 4, twice daily in month 5, and once daily in months 6 to 12. The main outcomes were immunologic rejection episodes and intraocular pressure (IOP) elevation (defined as >=24 mm Hg or >=10 mm Hg increase over the preoperative baseline level), assessed by the Kaplan-Meier survival analysis.The study included 325 eyes (99% were white, 96% had Fuchs dystrophy, and 9% had a previous glaucoma diagnosis). No eyes (0%) assigned to prednisolone versus 2 eyes (1.4%) assigned to fluorometholone experienced a possible (n = 1) or probable (n = 1) rejection episode (P = 0.17). Both rejection episodes resolved successfully with increased topical steroids. In the prednisolone arm, a significantly higher proportion exceeded the defined IOP elevation threshold (22% vs. 6%, P = 0.0005), and glaucoma medications were initiated or increased more often (17% vs. 5%, P = 0.0003). The most frequent reasons for discontinuing the assigned intervention were IOP management (n = 13 eyes assigned to prednisolone) or inflammation management (n = 3 eyes assigned to fluorometholone). One-year endothelial cell loss was comparable in both arms (30% vs. 31%, P = 0.50).DMEK has a remarkably low rejection episode rate (<1% through 1 year), as confirmed in this prospective randomized study. This provides a unique opportunity to reduce postoperative topical corticosteroid strength and thereby reduce the risk of steroid-associated complications.},
author = {Price, Marianne O. and Price, Francis W. and Kruse, Friedrich and Bachmann, Björn and Tourtas, Theofilos},
doi = {10.1097/ICO.0000000000000206},
faupublication = {yes},
journal = {Cornea},
note = {EVALuna2:21205},
pages = {880-6},
peerreviewed = {Yes},
title = {{Randomized} comparison of topical prednisolone acetate 1% versus fluorometholone 0.1% in the first year after descemet membrane endothelial keratoplasty},
volume = {33},
year = {2014}
}
@article{faucris.266497635,
abstract = {Purpose: Descemet membrane endothelial keratoplasty is often combined with phacoemulsification and intraocular lens implantation (DMEK + cataract/IOL triple procedure) in phakic patients. This procedure results in a refractive shift that is difficult to predict. The aim of this study was to evaluate the hypothesis that the refractive shift in the second eye follows the shift in the first eye. Methods: In this retrospective, single-center, consecutive case series, the refractive outcomes of 254 eyes of 127 patients who underwent DMEK + cataract/IOL triple procedure in both eyes for Fuchs endothelial corneal dystrophy have been analyzed. Main outcome measures were spherical equivalent outcome (shift calculations), best spectacle-corrected visual acuity, central corneal thickness, and posterior simulated keratometry. Results: The mean best spectacle-corrected visual acuity before surgery was 0.51 +/- 0.24 and increased to 0.19 +/- 0.15 (logMAR) after surgery (P < 0.001). After surgery, a mean hyperopic shift of 0.98 +/- 0.89 D was observed. The refractive shift was 1.03 +/- 0.93 D and 0.92 +/- 1.02 D, in the first and second eyes, respectively (P = 0.435). In a paired analysis, the mean difference of the refractive shift between the first and second eyes was 0.49 +/- 0.43 D. Conclusions: In our fellow eye comparison, the refractive shift after DMEK + cataract/IOL triple procedure in the second eye was comparable with the shift in the first eye. As a consequence, the refractive outcome of the first eye might serve as a reference for optimizing the refractive target in the second eye. Further studies investigating the influence of corneal parameters on refractive shift are needed for a more predictable lens power selection.},
author = {Augustin, Victor A. and Weller, Julia and Kruse, Friedrich and Tourtas, Theofilos},
doi = {10.1097/ICO.0000000000002602},
faupublication = {yes},
journal = {Cornea},
note = {CRIS-Team WoS Importer:2021-11-26},
pages = {883-887},
peerreviewed = {Yes},
title = {{Refractive} {Outcomes} {After} {Descemet} {Membrane} {Endothelial} {Keratoplasty} plus {Cataract}/{Intraocular} {Lens} {Triple} {Procedure}: {A} {Fellow} {Eye} {Comparison}},
volume = {40},
year = {2021}
}
@article{faucris.119746704,
abstract = {Purpose. Pseudoexfoliation (PEX) syndrome/glaucoma is a complex, late-onset disorder of the elastic fiber system. Strong genetic risk is conferred by the lysyl oxidase-like 1 (LOXL1) gene, but additional comodulating factors are necessary for the manifestation of the disease. The aim of this study was to analyze the effect of various PEX-associated pathogenic factors on the genotype-correlated expression of LOXL1 and elastin-related genes. Methods. Cultured human Tenon's capsule fibroblasts with high- and low-risk LOXL1 haplotypes were exposed to transforming growth factor (TGF)-?1, interleukin (IL)-6, homocysteine, oxidative stress, hypoxia, or ultraviolet (UV) radiation. Changes in the expression of LOXL1 and elastic constituents of PEX material and TGF-?1 were assessed by quantitative real-time PCR, Western blotting, immunohistochemistry, and electron microscopy. Results. Treatment of fibroblasts with TGF-?1, oxidative stress, UV light, and hypoxia induced a significant increase in expression levels of LOXL1 and elastic proteins, whereas the effect of IL-6 was limited to induction of elastic constituents. Immunohistochemistry and electron microscopy confirmed an upregulation of LOXL1 and elastic fiber proteins and their assembly into extracellular microfibrillar networks with focal aggregation of microfibrils into PEX-like fibrils on stimulation with TGF-?1 and oxidative stress. Basal and stimulated expression of LOXL1 mRNA and protein was slightly decreased in cells carrying the high-risk compared with the low-risk haplotype of LOXL1, but the differences between groups were statistically not significant. Conclusions. The findings support the notion that both genetic and nongenetic fibrogenic factors, particularly TGF-?1 and oxidative stress, may cooperate in the stable accumulation of PEX aggregates.},
author = {Zenkel, Matthias and Krysta, Anita and Pasutto, Francesca and Jünemann, Anselm and Kruse, Friedrich and Schlötzer-Schrehardt, Ursula},
doi = {10.1167/iovs.11-8361},
faupublication = {yes},
journal = {Investigative Ophthalmology & Visual Science},
note = {EVALuna2:9087},
pages = {8488-95},
peerreviewed = {Yes},
title = {{Regulation} of {Lysyl} {Oxidase}-like 1 ({LOXL1}) and {Elastin}-{Related} {Genes} by {Pathogenic} {Factors} {Associated} with {Pseudoexfoliation} {Syndrome}},
volume = {52},
year = {2011}
}
@article{faucris.119586104,
abstract = {To assess the reproducibility of manual graft preparation and evaluate the incidence rate and nature of structural anomalies of Descemet's membrane (DM) preventing successful graft preparation in DM endothelial keratoplasty (DMEK).Prospective, single-center, nonrandomized, consecutive case series.We analyzed 350 corneoscleral buttons from donors aged 18-95 years stored in Optisol-GS or Dulbecco's modified Eagle's medium and used for DMEK surgery in 343 consecutive patients with Fuchs' endothelial dystrophy or pseudophakic bullous keratopathy.Residual endothelial cell-DM complexes obtained after successful DM stripping for DMEK and whole donor corneas obtained after unsuccessful DM stripping were examined by transmission electron microscopy and immunohistochemistry.Accuracy of the cleavage plane between DM and corneal stroma and structural abnormalities of the DM-stroma interface.Uneventful manual separation without any disruption of DM was achieved in 335 of 350 donor corneas (95.7%) by use of a previously established bimanual submerged preparation technique. Correspondingly, the peeled DM specimens revealed a regular and smooth cleavage plane exposing the amorphous interfacial matrix on their anterior surface. Although 8 of 350 donor corneas (2.3%) showed focal adhesions of DM to the corneal stroma and developed isolated tears during stripping, preparation of the graft could be successfully completed. However, 7 of the 350 donor corneas (2.0%) showed extremely strong adhesion and multiple tears of DM, preventing successful preparation of the graft. These specimens revealed either ultrastructural (peg-like interlockings) or biochemical abnormalities (increased staining intensities for adhesive glycoproteins) along the DM-stroma interface.Using an appropriate technique, manual preparation of grafts for DMEK with reproducible tissue qualities is possible in the vast majority (98%) of donor corneas. Although a relatively rare phenomenon, interindividual variations in DM structure and composition may be responsible for failure of graft preparation in about 2% of donor corneas.The authors have no proprietary or commercial interest in any of the materials discussed in this article.},
author = {Schlötzer-Schrehardt, Ursula and Bachmann, Björn and Tourtas, Theofilos and Cursiefen, Claus and Zenkel, Matthias and Roessler, Kathrin and Kruse, Friedrich},
doi = {10.1016/j.ophtha.2013.06.038},
faupublication = {yes},
journal = {Ophthalmology},
note = {EVALuna2:21132},
pages = {1769-77},
peerreviewed = {Yes},
title = {{Reproducibility} of graft preparations in {Descemet}'s membrane endothelial keratoplasty},
volume = {120},
year = {2013}
}
@article{faucris.262415443,
abstract = {Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes coronavirus disease 2019 (COVID-19), affects the pulmonary systems via angiotensin-converting enzyme-2 (ACE-2) receptor, being an entry to systemic infection. As COVID-19 disease features ACE-2 deficiency, a link to microcirculation is proposed. Optical coherence tomography angiography (OCT-A) enables non-invasive analysis of retinal microvasculature. Thus, an impaired systemic microcirculation might be mapped on retinal capillary system. As recent OCT-A studies, analyzing microcirculation in two subdivided layers, yielded contrary results, an increased subdivision of retinal microvasculature might offer an even more fine analysis. The aim of the study was to investigate retinal microcirculation by OCT-A after COVID-19 infection in three subdivided layers (I). In addition, short-term retinal affections were monitored during COVID-19 disease (II). Considering (I), a prospective study (33 patientspost−COVID and 28 controls) was done. Macula and peripapillary vessel density (VD) were scanned with the Spectralis II. Macula VD was measured in three layers: superficial vascular plexus (SVP), intermediate capillary plexus (ICP), and deep capillary plexus (DCP). Analysis was done by the EA-Tool, including an Anatomical Positioning System and an analysis of peripapillary VD by implementing Bruch's membrane opening (BMO) landmarks. Overall, circular (c1, c2, and c3) and sectorial VD (s1-s12) was analyzed. Considering (II), in a retrospective study, 29 patients with severe complications of COVID-19 infection, hospitalized at the intensive care unit, were monitored for retinal findings at bedside during hospitalization. (I) Overall (p = 0.0133) and circular (c1, p = 0.00257; c2, p = 0.0067; and c3, p = 0.0345). VD of the ICP was significantly reduced between patientspost−COVID and controls, respectively. Overall (p = 0.0179) and circular (c1, p = 0.0189) peripapillary VD was significantly reduced between both groups. Subgroup analysis of hospitalized vs. non-hospitalized patientspost−COVID yielded a significantly reduced VD of adjacent layers (DCP and SVP) with increased severity of COVID-19 disease. Clinical severity parameters showed a negative correlation with VD (ICP) and peripapillary VD. (II) Funduscopy yielded retinal hemorrhages and cotton wool spots in 17% of patients during SARS-CoV-2 infection. As VD of the ICP and peripapillary regions was significantly reduced after COVID-19 disease and showed a link to clinical severity markers, we assume that the severity of capillary impairment after COVID-19 infection is mapped on retinal microcirculation, visualized by non-invasive OCT-A.},
author = {Hohberger, Bettina and Ganslmayer, Marion and Lucio, Marianna and Kruse, Friedrich and Hoffmanns, Jakob and Moritz, Michael and Rogge, Lennart and Heltmann, Felix and Szewczykowski, Charlotte and Fürst, Julia and Raftis, Maximilian and Bergua, Antonio and Zenkel, Matthias and Gießl, Andreas and Schlötzer-Schrehardt, Ursula and Lehmann, Paul and Strauss, Richard and Mardin, Christian and Herrmann, Martin},
doi = {10.3389/fmed.2021.676554},
faupublication = {yes},
journal = {Frontiers in Medicine},
keywords = {COVID-19; macula; microcirculation; OCT-angiography; optic nerve head; retina; SARS-CoV-2},
note = {CRIS-Team Scopus Importer:2021-08-06},
peerreviewed = {Yes},
title = {{Retinal} {Microcirculation} as a {Correlate} of a {Systemic} {Capillary} {Impairment} {After} {Severe} {Acute} {Respiratory} {Syndrome} {Coronavirus} 2 {Infection}},
volume = {8},
year = {2021}
}
@article{faucris.283619115,
abstract = {Purpose: To investigate the incidence of postoperative hypotony, and risk factors for the development of hypotony in eyes who had undergone XEN Gel Stent implantation. Methods: In this retrospective, single-centre case series, medical records of 170 consecutive eyes who had undergone XEN Gel Stent implantation with or without simultaneous phacoemulsification for primary or secondary open angle glaucoma were analysed. Primary outcome parameters were the incidence of postoperative hypotony and potential risk factors for its development, and secondary parameters were pre- and postoperative visual acuity, intraocular pressure (IOP), and number of IOP-lowering eye drops. Results: Postoperative hypotony ≤ 6 mmHg occurred in 57% of eyes. Hypotony was without complications in 70.1%, 13.4% had transient complications with spontaneous resolution, and 16.5% had complications requiring treatment. Mean visual acuity logMAR before surgery accounted for 0.47 ± 0.46 in all eyes and 0.47 ± 0.48 at the 4-week visit. There was no significant difference of BCVA in the group of eyes with and without postoperative hypotony before and after surgery. The mean IOP before surgery was 24.6 ± 8.4 mmHg and decreased significantly to 18.4 ± 10.2 after 4 weeks. Eyes with an axial length over 24.3 mm had a threefold increased risk for postoperative hypotony (OR 3.226, 95% confidence interval 1.121–9.279). This risk was decreased in eyes with simultaneous cataract surgery (OR 0.483, 95% confidence interval 0.258–0.903). Conclusion: In our sample, postoperative hypotony was a common complication after XEN Gel Stent implantation, but serious, persistent complications were rare. A longer axial length predisposes the eye for the development of hypotony.[Figure not available: see fulltext.].},
author = {Galimi, Maria E. and Weller, Julia and Kruse, Friedrich and Lämmer, Robert},
doi = {10.1007/s00417-022-05831-2},
faupublication = {yes},
journal = {Graefes Archive For Clinical and Experimental Ophthalmology},
keywords = {Glaucoma; Hypotony; Minimal invasive glaucoma surgery (MIGS); XEN Gel Stent},
note = {CRIS-Team Scopus Importer:2022-10-21},
peerreviewed = {Yes},
title = {{Risk} factors for ocular hypotony after {XEN} {Gel} {Stent} implantation},
year = {2022}
}
@inproceedings{faucris.207813880,
author = {Pasutto, Francesca and Zenkel, Matthias and Berner, Daniel and Uebe, Steffen and Ekici, Arif Bülent and Kruse, Friedrich and Reis, André and Schlötzer-Schrehardt, Ursula},
faupublication = {yes},
note = {EVALuna2:34745},
peerreviewed = {Yes},
title = {{RNA}-seq and pathway analysis of ocular tissues in {PEX} patients and healthy subjects},
volume = {59},
year = {2018}
}
@article{faucris.306933027,
abstract = {Fuchs endothelial corneal dystrophy (FECD) is the most common inherited corneal disease. Fibrillar focal excrescences called guttae and corneal edema due to corneal endothelial cell death result in progressive vision loss. Multiple genetic variants have been reported, but the pathogenesis of FECD is not fully understood. In this study, we used RNA-Seq to analyze differential gene expression in the corneal endothelium obtained from patients with FECD. Differential expression analysis of transcriptomic profiles revealed that expression of 2366 genes (1092 upregulated and 1274 downregulated genes) was significantly altered in the corneal endothelium of patients with FECD compared to healthy subjects. Gene ontology analysis demonstrated an enrichment of genes involved in extracellular matrix (ECM) organization, response to oxidative stress, and apoptotic signaling. Several pathway analyses consistently indicated the dysregulation of ECM-associated pathways. Our differential gene expression findings support the previously proposed underlying mechanisms, including oxidative stress and apoptosis of endothelial cells, as well as the phenotypic clinical FECD hallmark of ECM deposits. Further investigation focusing on differentially expressed genes related to these pathways might be beneficial for elucidating mechanisms and developing novel therapies.},
author = {Nakagawa, Tatsuya and Tokuda, Yuichi and Nakano, Masakazu and Komori, Yuya and Hanada, Naoya and Tourtas, Theofilos and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich and Tashiro, Kei and Koizumi, Noriko and Okumura, Naoki},
doi = {10.1038/s41598-023-35468-y},
faupublication = {yes},
journal = {Scientific Reports},
note = {CRIS-Team Scopus Importer:2023-06-30},
peerreviewed = {Yes},
title = {{RNA}-{Seq}–based transcriptome analysis of corneal endothelial cells derived from patients with {Fuchs} endothelial corneal dystrophy},
volume = {13},
year = {2023}
}
@inproceedings{faucris.282437974,
address = {ROCKVILLE},
author = {Nakagawa, Tatsuya and Okumura, Naoki and Komori, Yuya and Hanada, Naoya and Tokuda, Yuichi and Nakano, Masakazu and Tourtas, Theofilos and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich and Koizumi, Noriko},
booktitle = {INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE},
doi = {10.21203/rs.3.rs-2253336/v1},
faupublication = {yes},
note = {CRIS-Team WoS Importer:2022-09-30},
peerreviewed = {unknown},
publisher = {ASSOC RESEARCH VISION OPHTHALMOLOGY INC},
title = {{RNA}-{Seq} based transcriptome analysis of corneal endothelial cells derived from the patients with {Fuchs} endothelial corneal dystrophy},
year = {2022}
}
@article{faucris.119636044,
abstract = {Keratoconjunctivitis sicca is one of the most common ocular diseases world-wide. These patients suffer from severe symptoms which lead to an extremely reduced quality of life. Dry eye syndrome constitutes a major diagnostic and therapeutic challenge to all ophthalmologists because there is often a discrepancy between objective ocular signs and subjective symptoms of the patients. Furthermore, there exist only few causal therapeutic options. The physician-patient relationship plays an outstanding role in this condition. For the treatment of moderate to severe dry eye syndrome, special dry eye clinics have proved to be extremely useful. For follow-up measurements as well as the realisation of evidence-based medicine and quality control, it is a fundamental necessity to document symptoms, signs and therapy of these patients in order to optimise therapeutic strategies. For this purpose, we have developed special forms and standardised questionnaires for the individual documentation of medical history and diagnostic findings. To objectively assess the patient's complaints we use the "ocular surface disease index" (OSDI score). Only the establishment of standardised diagnostic and therapeutic algorithms with the help of special forms and questionnaires can help in the long run to improve the treatment of these severely affected patients.},
author = {Jacobi, Christina and Bellios, N. and Jacobi, A. and Kruse, Friedrich and Cursiefen, Claus},
doi = {10.1055/s-0029-1245272},
faupublication = {yes},
journal = {Klinische Monatsblätter für Augenheilkunde},
note = {EVALuna2:21043},
pages = {226-33},
peerreviewed = {Yes},
title = {{Screening} questionnaire for documentation of medical history and diagnostic findings in dry eye disease},
volume = {228},
year = {2011}
}
@article{faucris.123401784,
abstract = {Transplantation of a donor cornea to restore vision is the most frequently performed transplantation in the world. Corneal endothelial cells (CEC) are crucial for the outcome of a graft as they maintain corneal transparency and avoid graft failure due to corneal opaqueness. Given the characteristic of being a monolayer and in direct contact with culture medium during cultivation in eye banks, CEC are specifically suitable for gene therapeutic approaches prior to transplantation. Recombinant adeno-associated virus 2 (rAAV2) vectors represent a promising tool for gene therapy of CEC. However, high vector titers are needed to achieve sufficient gene expression. One of the rate-limiting steps for transgene expression is the conversion of single-stranded (ss-) DNA vector genome into double-stranded (ds-) DNA. This step can be bypassed by using self-complementary (sc-) AAV2 vectors. Aim of this study was to compare for the first time transduction efficiencies of ss- and scAAV2 vectors in CEC. For this purpose AAV2 vectors containing enhanced green fluorescent protein (GFP) as transgene were used. Both in CEC and in donor corneas, transduction with scAAV2 resulted in significantly higher transgene expression compared to ssAAV2. The difference in transduction efficiency decreased with increasing vector titer. In most cases, only half the vector titer of scAAV2 was required for equal or higher gene expression rates than those of ssAAV2. In human donor corneas, GFP expression was 64.7±11.3% (scAAV) and 38.0±8.6% (ssAAV) (p<0.001), respectively. Furthermore, transduced cells maintained their viability and showed regular morphology. Working together with regulatory authorities, a translation of AAV2 vector-mediated gene therapy to achieve a temporary protection of corneal allografts during cultivation and transplantation could therefore become more realistic.},
author = {Gruenert, Anja K. and Czugala, Marta and Mueller, Chris and Schmeer, Marco and Schleef, Martin and Kruse, Friedrich and Fuchsluger, Thomas},
doi = {10.1371/journal.pone.0152589},
faupublication = {yes},
journal = {PLoS ONE},
note = {EVALuna2:21299},
pages = {e0152589},
peerreviewed = {Yes},
title = {{Self}-{Complementary} {Adeno}-{Associated} {Virus} {Vectors} {Improve} {Transduction} {Efficiency} of {Corneal} {Endothelial} {Cells}},
volume = {11},
year = {2016}
}
@inproceedings{faucris.228586754,
address = {ROCKVILLE},
author = {Fuchsluger, Thomas Armin and Thieme, Daniel and Kruse, Friedrich and Mahajan, Siddharth},
booktitle = {INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE},
date = {2019-04-28/2019-05-02},
faupublication = {yes},
note = {CRIS-Team WoS Importer:2019-11-01},
peerreviewed = {unknown},
publisher = {ASSOC RESEARCH VISION OPHTHALMOLOGY INC},
title = {{Silencing} of pro-apoptotic proteins as anti-apoptotic therapy for corneal endothelium},
venue = {Vancouver},
year = {2019}
}
@article{faucris.119622844,
abstract = {To analyze the short- and intermediate-term results of simultaneous transplantation of amniotic membrane with high-risk keratoplasty.Between January 2002 and February 2004, a simultaneous amniotic membrane patch was transplanted with penetrating keratoplasty in 16 eyes of 16 patients. In 13 eyes, a soft contact lens was applied afterward. Corneal perforation was present in 10 of 14 eyes with emergency keratoplasty. Five patients received systemic immunosuppressive medication for 4-6 months after penetrating keratoplasty.The amniotic membrane patch fell off after 8 ± 3 (range: 4-14) days without residual tissue except in 1 case. In 15 of 16 eyes, the epithelium was completely closed after 10 ± 8 (range: 4-30) days. In 3 eyes, recurrence of the epithelial defect occurred after 3-6 months. During a follow-up period of 18 ± 6 months, 13 of 16 corneal grafts were clear.Simultaneous amniotic membrane transplantation and penetrating keratoplasty may improve the prognosis of corneal graft in eyes with risk of epithelial healing problems.},
author = {Seitz, Berthold and Das, Sujata and Sauer, Rolf and Hofmann-Rummelt, Carmen and Beckmann, Matthias and Kruse, Friedrich},
doi = {10.1097/ICO.0b013e3181eae8ea},
faupublication = {yes},
journal = {Cornea},
note = {EVALuna2:17178},
pages = {269-72},
peerreviewed = {Yes},
title = {{Simultaneous} amniotic membrane patch in high-risk keratoplasty},
volume = {30},
year = {2011}
}
@article{faucris.106133544,
abstract = {To evaluate the feasibility of using a single donor cornea for 2 recipients by combining deep anterior lamellar keratoplasty (DALK) and Descemet's membrane endothelial keratoplasty (DMEK) surgeries on the same day.Single-center, nonrandomized, prospective, interventional case series.Twelve consecutive donor corneas were scheduled for split cornea transplantation combining DALK for a keratoconus patient and DMEK for a Fuchs' endothelial dystrophy patient on the same surgery day.First, a big-bubble DALK procedure was performed for the keratoconus eye. When bare Descemet's membrane was prepared successfully requiring no conversion to penetrating keratoplasty (PK), then during surgery the donor, endothelium-Descemet's membrane layer was removed and stored for subsequent DMEK in a second patient, and the remaining anterior lamella of the donor cornea was used to complete the DALK surgery. Afterward, a DMEK procedure was performed on the second patient with Fuchs' endothelial dystrophy, grafting the stored endothelium-Descemet's membrane layer of the original donor button.Success of using a single donor cornea for 2 recipient eyes, best spectacle-corrected visual acuity (BSCVA), and complication rates within 6 months follow-up.A single donor cornea could be used for 2 recipients in 10 of 12 donor buttons (83%). In 2 cases (17%), the DALK procedure had to be converted to PK requiring a full-thickness corneal graft. Therefore, 10 donor corneas (45%) could be saved. Six months after surgery, mean BSCVA was 20/35 (range, 20/50-20/25) in 10 eyes that underwent successful DALK, 20/50 (range, 20/63-20/40) in 2 eyes that underwent conversion from DALK to PK, and 20/31 (range, 20/50-20/16) in 10 eyes that underwent DMEK. Postoperative complications after DALK included Descemet's folds in 3 eyes (30%) and epitheliopathy in 2 eyes (20%). After DMEK, partial graft detachment occurred in 5 eyes (50%) and was managed successfully with intracameral air reinjection. All corneas remained clear up to 6 months after surgery.Split use of donor corneal tissue for combined DALK and DMEK procedures in 2 recipients on the same surgery day is a promising strategy to reduce donor shortage and cost in corneal transplantation surgery in the future.},
author = {Heindl, Ludwig M. and Riss, Stephan and Bachmann, Bjoern O. and Laaser, Kathrin and Kruse, Friedrich and Cursiefen, Claus},
doi = {10.1016/j.ophtha.2010.05.025},
faupublication = {yes},
journal = {Ophthalmology},
note = {EVALuna2:21012},
pages = {294-301},
peerreviewed = {Yes},
title = {{Split} cornea transplantation for 2 recipients: a new strategy to reduce corneal tissue cost and shortage},
volume = {118},
year = {2011}
}
@article{faucris.106562104,
abstract = {To evaluate the feasibility of split cornea transplantation for 2 recipients by combining deep anterior lamellar keratoplasty (DALK) and Descemet membrane endothelial keratoplasty (DMEK).Interventional case series.Fifty consecutive eyes with anterior stromal disease suitable for DALK and 50 eyes with endothelial disease suitable for DMEK were scheduled for split cornea transplantation combining both procedures within 72 hours. Main outcome measures included success of using a single donor cornea for 2 recipients, best spectacle-corrected visual acuity (BSCVA), and complication rates within 6 months' follow-up.A single donor cornea could be used for 2 recipients in 47 cases (94%). In 3 eyes (6%), the DALK procedure had to be converted to penetrating keratoplasty (PK) requiring a full-thickness corneal graft. Thereby, 47 donor corneas (47%) could be saved. Six months after surgery, mean BSCVA was 20/36 in the 47 eyes that underwent successful DALK, 20/50 in the 3 eyes that underwent conversion from DALK to PK, and 20/29 in the 50 eyes that underwent DMEK. Postoperative complications after DALK included Descemet folds in 5 eyes (11%) and epitheliopathy in 3 eyes (6%). After DMEK, partial graft detachment occurred in 26 eyes (52%) and was managed successfully with intracameral air reinjection. All corneas remained clear up to 6 months after surgery. No intraocular infections occurred.Split use of donor corneal tissue for combined DALK and DMEK procedures in 2 recipients within 3 subsequent days is a feasible approach to reduce donor shortage in corneal transplantation in the future.},
author = {Heindl, Ludwig M. and Riss, Stephan and Laaser, Kathrin and Bachmann, Bjoern O. and Kruse, Friedrich and Cursiefen, Claus},
doi = {10.1016/j.ajo.2011.03.021},
faupublication = {yes},
journal = {American Journal of Ophthalmology},
note = {EVALuna2:21041},
pages = {523-532.e2},
peerreviewed = {Yes},
title = {{Split} cornea transplantation for 2 recipients - review of the first 100 consecutive patients},
volume = {152},
year = {2011}
}
@article{faucris.110640464,
abstract = {In Descemet membrane endothelial keratoplasty (DMEK), lamellar splitting of the Descemet membrane (DM) may occur during stripping of host DM, leaving residual DM on the recipient's DMEK interface. The purpose of this study was to determine the incidence rate of lamellar splitting of DM during DMEK and to describe the ultrastructure of DM in these eyes.Retrospective consecutive case series.setting: Institutional, single-center.Total of 664 eyes with Fuchs endothelial corneal dystrophy (FECD) scheduled for primary DMEK.DMEK.The incidence rate of lamellar DM splitting in the recipients' eyes; ultrastructural alterations of stripped DM specimens (transmission electron microscopy); preoperative best-corrected visual acuity (BCVA), central corneal thickness (CCT), and prevalence of diabetes mellitus.Sixty-three of 664 eyes (9.5%) with FECD showed lamellar splitting of DM resulting in the dissociation of 2 separate layers. Transmission electron microscopy revealed accumulations of banded and wide-spaced collagen between the thicker posterior banded layer and the thin anterior banded layer, which is adhesive to the corneal stroma. Lamellar splitting occurred along these abnormal collagen inclusions, demarcating the borderline between both layers of DM.Lamellar DM splitting occurs during DM stripping in almost 10% of eyes with FECD. This phenomenon appears to be caused by abnormal collagenous material deposits at the borderline between anterior and posterior layers of DM.},
author = {Weller, Julia M. and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich and Tourtas, Theofilos},
doi = {10.1016/j.ajo.2016.08.037},
faupublication = {yes},
journal = {American Journal of Ophthalmology},
note = {EVALuna2:21303},
pages = {1-6},
peerreviewed = {Yes},
title = {{Splitting} of the {Recipient}'s {Descemet} {Membrane} in {Descemet} {Membrane} {Endothelial} {Keratoplasty}-{Ultrastructure} and {Clinical} {Relevance}},
volume = {172},
year = {2016}
}
@article{faucris.106587184,
author = {Kopsachilis, Nikolaos and Tsinopoulos, Ioannis and Tourtas, Theofilos and Kruse, Friedrich and Cursiefen, Claus},
doi = {10.1111/j.1442-9071.2010.02448.x},
faupublication = {yes},
journal = {Clinical and Experimental Ophthalmology},
note = {EVALuna2:20990},
pages = {372-5},
peerreviewed = {Yes},
title = {{Spontaneous} resolution of corneal decompensation after big-bubble deep anterior lamellar keratoplasty with intraoperative {Descemet}'s membrane perforation},
volume = {39},
year = {2011}
}
@article{faucris.106397104,
author = {Schlötzer-Schrehardt, Ursula and Kruse, Friedrich},
doi = {10.1007/s00347-017-0476-0},
faupublication = {yes},
journal = {Ophthalmologe},
note = {EVALuna2:21337},
pages = {296-297},
peerreviewed = {Yes},
title = {{Stem} cell-based approaches to diseases of the ocular surface},
volume = {114},
year = {2017}
}
@article{faucris.119504044,
abstract = {Limbal stem cell deficiency is a painful and potentially blinding disease. Cultured limbal epithelial transplantation (CLET) is frequently performed for corneal surface reconstruction with variable clinical success. This work summarizes recent developments and trends that have the potential to increase safety and efficacy of CLET in the future. Apart from gradual transition to xenobiotic-free culture systems, novel biofunctional scaffolds presenting components of stem cell microenvironments aim at promoting long-term maintenance of stem cells in vitro and after transplantation. Hair follicles and other tissues may serve as autologous sources of adult stem cells in bilateral ocular surface disease. However, despite all progress made in the fields of tissue engineering and cell therapy, it is unlikely that CLET will yield fully satisfactory clinical results until the factors that govern limbal stem cell maintenance and differentiation are identified.},
author = {Menzel-Severing, Johannes and Kruse, Friedrich and Schlötzer-Schrehardt, Ursula},
doi = {10.1016/j.jcjo.2012.11.009},
faupublication = {yes},
journal = {Canadian Journal of Ophthalmology-Journal Canadien D Ophtalmologie},
note = {EVALuna2:21130},
pages = {13-21},
peerreviewed = {Yes},
title = {{Stem} cell-based therapy for corneal epithelial reconstruction: present and future},
volume = {48},
year = {2013}
}
@article{faucris.208944967,
abstract = {PURPOSE: Assessment of the diagnostic ability of segmented macular inner retinal layer thickness and peripapillary retinal nerve fiber layer (pRNFL) measured by spectral-domain optical coherence tomography (SD-OCT) in patients with normal-tension (NT) and high-tension (HT) perimetric and preperimetric glaucoma.
METHODS: The 212 participants included 45 healthy subjects, 55 patients with ocular hypertension, 56 patients with preperimetric glaucoma, and 56 patients with perimetric glaucoma. The preperimetric and perimetric groups were further subdivided into NT and HT groups. Sectoral and global thickness of macular retinal nerve fiber layer (mRNFL), ganglion cell layer (mGCL), inner plexiform layer (mIPL), ganglion cell complex (mGCC), and pRNFL were measured using SD-OCT (Spectralis, Heidelberg Engineering, Germany). Diagnostic performance was ascertained by sectoral and global comparison of the sensitivities at specificity ≥ 95%.
RESULTS: For all layers, the largest thickness decrease was reported in the HT perimetric group. In all groups, the sensitivities of mGCL showed a comparable diagnostic value to pRNFL in order to distinguish between healthy subjects and glaucoma patients. In the perimetric group, mGCL (85.7%) exhibited higher sensitivities than mRNFL (78.6%) and mGCC (78.6%). Both mRNFL and pRNFL demonstrated equal diagnostic performance in the HT perimetric group (88.5 and 96.2%), in the NT groups, mRNFL was inferior to all other layers.
CONCLUSION: The sensitivities of mGCL and mRNFL were comparable to the sensitivities of pRNFL. In clinical application, mGCL and mRNFL, with a focus on the temporal and inferior sectors, may provide a convincing supplementation to pRNFL.
CLINICAL TRIAL REGISTRATION: Erlangen Glaucoma Registry www.clinicaltrials.gov ID: NCT00494923.},
author = {Edlinger, Florian S. M. and Schrems-Hösl, Laura-Maria and Mardin, Christian Y. and Lämmer, Robert and Kruse, Friedrich and Schrems, Wolfgang},
doi = {10.1007/s00417-018-3944-6},
faupublication = {yes},
journal = {Graefes Archive For Clinical and Experimental Ophthalmology},
note = {EVALuna2:34747},
pages = {1245-1256},
peerreviewed = {Yes},
title = {{Structural} changes of macular inner retinal layers in early normal-tension and high-tension glaucoma by spectral-domain optical coherence tomography},
volume = {256},
year = {2018}
}
@inproceedings{faucris.228450057,
address = {ROCKVILLE},
author = {Galimi, Maria Elena and Weller, Julia M. and Kruse, Friedrich and Augustin, Victor A. and Tourtas, Theofilos},
booktitle = {INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE},
date = {2019-04-28/2019-05-02},
faupublication = {yes},
note = {CRIS-Team WoS Importer:2019-10-29},
peerreviewed = {unknown},
publisher = {ASSOC RESEARCH VISION OPHTHALMOLOGY INC},
title = {{Supplementation} of amphotericin {B} in {Optisol}-{GS} for {Descemet} membrane endothelial keratoplasty ({DMEK})},
venue = {Vancouver, CANADA},
year = {2019}
}
@article{faucris.220849303,
abstract = {To assess corneal endothelial cell (CEC) quantity and quality in eye-bank prepared lamellar grafts for Descemet Membrane Endothelial Keratoplasty (DMEK) from organ cultured donor corneas that have not undergone de-swelling by media supplementation with dextran. Prior to graft preparation, corneas that had not undergone de-swelling (n = 30) were placed into fresh storage medium without dextran (KMI). Corneas in the control group (n = 30) were placed in dehydration medium containing 5% dextran (KMII). Subtotal stripping of Descemet’s membrane (DM) was performed manually. Following graft preparation, 10 corneas of each group were cultured further in their respective medium for 24 h, 72 h, or 120 h, respectively. Before and after DM stripping, as well as at the end of culture, CEC numbers were obtained. At the end of culture, CEC morphology was graded using a scoring system and CEC metabolism was assessed by detection of adenosine triphosphate. At 24 h after DM stripping, mean CEC counts (in cells/mm2) were 2204 in corneas stored in KMII, and 2391 in corneas stored in KMI (p = 0.003). This corresponds to a mean relative CEC loss of 12.4% with dextran versus 9.7% without dextran (p = 0.04). At 72 h, CEC counts were 1946 in KMII, and 2289 in KMI (p = 0.004). This corresponds to CEC loss of 23% with dextran versus 14% without dextran (p = 0.009). At 120 h, CEC counts were 2047 in KMII, and 2230 in KMI (p = 0.14). This corresponds to CEC loss of 22.7% with dextran versus 17.2% without dextran (p = 0.14). Also, at 120 h after DM stripping, 6/10 corneas fell below a threshold of 2000 cells/mm2 if stored in medium containing dextran, versus 1/9 corneas if stored without dextran (p = 0.003). Morphological assessment of CEC quality revealed equal scores for cell polymorphism (median = 1), granulation (median = 0) and segmentation (median = 1) in all groups. Lower ATP/protein ratios were observed in corneas stored in medium without dextran at 24 h (p < 0.001), 72 h (p < 0.001), and 120 h (p = 0.02). Abandoning the use of dextran in corneas destined for DMEK surgery leads to increased CEC counts and thereby serves to reduce tissue loss.},
author = {Salla, Sabine and Kruse, Friedrich and Walter, Peter and Menzel-Severing, Johannes},
doi = {10.1007/s10561-019-09757-8},
faupublication = {yes},
journal = {Cell and Tissue Banking},
keywords = {Corneal transplantation; DMEK; Lamellar keratoplasty; Tissue banking},
note = {CRIS-Team Scopus Importer:2019-06-18},
pages = {193-200},
peerreviewed = {Yes},
title = {{Supplementation} of organ culture medium with dextran is not required in pre-stripped human donor tissue for {DMEK} surgery},
volume = {20},
year = {2019}
}
@article{faucris.122798324,
abstract = {The unfolded protein response (UPR) is believed to play a role in the pathogenesis of Fuchs' endothelial corneal dystrophy (FECD). The purpose of this study was to investigate whether unfolded proteins accumulate in the corneal endothelium in FECD and if they are involved in triggering cell death.Descemet's membranes with corneal endothelial cells (CECs) were obtained during keratoplasty, and expression of aggresomes, type 1 collagen, fibronectin, and agrin was evaluated. Endoplasmic reticulum (ER) stress of immortalized human CECs from non-FECD subjects and from FECD patients (iHCEC and iFECD, respectively) were evaluated. The effect of MG132-mediated aggresome formation on the UPR and intrinsic pathway and the effect of mitochondrial damage on UPR were also examined. The effect of CHOP knockdown on the ER stress-mediated intrinsic pathway was also evaluated.Aggresome formation was higher in iFECD than in iHCEC and was colocalized with type 1 collagen, fibronectin, and agrin. GRP78, phosphorylated IRE1, PERK, and CHOP showed higher activation in iFECD than in iHCEC. MG132-mediated aggresome formation upregulated ER stress sensors, the mitochondrial membrane potential drop, cytochrome c release to the cytoplasm, and activation of caspase-9 and -3. By contrast, staurosporine-mediated mitochondrial damage did not induce ER stress. Knockdown of CHOP attenuated the ER stress-induced cleavage of caspase-9, which is caused by intrinsic pathway activation.Excessive synthesis of extracellular matrix proteins induced unfolded protein accumulation in FECD. Prolonged ER stress-mediated cell death, occurring via the intrinsic apoptotic signaling pathway, therefore might be associated with the pathogenesis of FECD.},
author = {Okumura, Naoki and Kitahara, Miu and Okuda, Hirokazu and Hashimoto, Keisuke and Ueda, Emi and Nakahara, Makiko and Kinoshita, Shigeru and Young, Robert D. and Quantock, Andrew J. and Tourtas, Theofilos and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich and Koizumi, Noriko},
doi = {10.1167/iovs.16-21023},
faupublication = {yes},
journal = {Investigative Ophthalmology & Visual Science},
note = {EVALuna2:21347},
pages = {3697-3707},
peerreviewed = {Yes},
title = {{Sustained} {Activation} of the {Unfolded} {Protein} {Response} {Induces} {Cell} {Death} in {Fuchs}' {Endothelial} {Corneal} {Dystrophy}},
volume = {58},
year = {2017}
}
@inproceedings{faucris.223404043,
author = {Voigt, Monika and Thieme, Daniel and Küng, Florian and Schubert, Dirk W. and Kruse, Friedrich and Fuchsluger, Thomas},
booktitle = {Deutsche Gesellschaft für Biomaterialien},
date = {2018-11-08/2018-11-10},
faupublication = {yes},
keywords = {biomaterialien, espinning suture retension},
peerreviewed = {unknown},
title = {{Suture} retension test method for opthalmological materials},
venue = {Braunschweig},
year = {2018}
}
@article{faucris.120714264,
abstract = {: Tear film hyperosmolarity is recognized as an important pathogenetic factor in dry eye syndrome, but difficulties in its measurement have limited its utility in the recent past. This prospective, nonrandomized, clinical single-center study investigates the osmolarity in tear samples of patients with keratoconjunctivitis sicca compared with healthy controls.: One hundred thirty-three patients [aged 58 years (51-64 years), 86 women and 47 men] with moderate to severe keratoconjunctivitis sicca and 95 controls [aged 52 years (48-61 years), 55 women and 40 men] were enrolled in the trial. Tear samples were collected directly from the inferior lateral tear meniscus. Inclusion criteria were a tear breakup time of less than 5 seconds, a Schirmer test with anesthesia less than 5 mm, and positive symptoms (Ocular Surface Disease Index score > 83). Tear film osmolarity was analyzed by the TearLab osmometer.: In our study, patients with moderate to severe keratoconjunctivitis sicca showed a tear film osmolarity of 320 mOsmol/L (301-324 mOsmol/L). The results of the control group were 301 mOsmol/L (298-304 mOsmol/L). Our results revealed a significantly higher tear film osmolarity in patients with moderate to severe keratoconjunctivitis sicca compared with the control group. The sensitivity was 87%, and the specificity was 81%.: Our results approved the referent value in moderate to severe dry eye of approximately 316 mOsmol/L, as described in the literature. The results showed a significantly higher tear film osmolarity in patients with severe keratoconjunctivitis sicca compared with the healthy controls. Testing tear film osmolarity can be a very effective objective diagnostic tool in the diagnosis of dry eye disease.},
author = {Jacobi, Christina and Jacobi, Arnd and Kruse, Friedrich and Cursiefen, Claus},
doi = {10.1097/ICO.0b013e31821de383},
faupublication = {yes},
journal = {Cornea},
note = {EVALuna2:21036},
pages = {1289-92},
peerreviewed = {Yes},
title = {{Tear} film osmolarity measurements in dry eye disease using electrical impedance technology},
volume = {30},
year = {2011}
}
@article{faucris.110879384,
abstract = {To evaluate rates of changes per year of central corneal thickness after antiglaucomatous drug administration with ?-blockers, prostaglandin analogs, and carbonic anhydrase inhibitors monotherapy and combined topical antiglaucomatous therapy, in a cohort of patients with ocular hypertension, glaucoma suspects, and patients with perimetric glaucoma as compared with normal controls.This retrospective single-center study included 130 eyes as healthy controls, 121 eyes of ocular hypertensive patients, 105 eyes of glaucoma suspects, and 49 eyes of perimetric glaucoma patients. All patients underwent standard automated perimetry, 24-hour intraocular pressure profile, optic disc photography, and optical coherence pachymetry (OCP; Heidelberg Engineering). The cohort was divided into 8 groups on the basis of topical antiglaucomatous medication. Linear regression analysis was conducted to analyze the relationship between central corneal thickness and exposure to antiglaucomatous medication during the follow-up.Central corneal thickness did not change during the follow-up for investigated diagnostic subgroups. There was a statistically significant decrease in central corneal thickness for eyes treated with prostaglandin monotherapy (-3.1 ?m/y for left eye), and a combined therapy with prostaglandins, carbonic anhydrase inhibitors, and ?-blockers (-5.8 and -3.8 ?m/y for right and left eye, respectively).We recommend regular measurements before and during therapy with prostaglandin monotherapy and a combined therapy with prostaglandins, carbonic anhydrase inhibitors, and ?-blockers. Follow-up intraocular pressure measurements may be underestimated for eyes treated with the aforementioned treatment regimens if central corneal thickness is not measured on a regular basis.},
author = {Schrems, Wolfgang and Schrems-Hösl, Laura-Maria and Mardin, Christian Y. and Horn, Folkert and Jünemann, Anselm and Kruse, Friedrich and Braun, Joachim M. and Lämmer, Robert},
doi = {10.1097/IJG.0000000000000190},
faupublication = {yes},
journal = {Journal of Glaucoma},
note = {EVALuna2:21177},
pages = {274-80},
peerreviewed = {Yes},
title = {{The} {Effect} of {Long}-term {Antiglaucomatous} {Drug} {Administration} on {Central} {Corneal} {Thickness}},
volume = {25},
year = {2016}
}
@article{faucris.123497264,
abstract = {Limbal stem cells reside in a highly specialized complex microenvironment that is known as the stem cell niche, an anatomically protected region at the bottom of the Palisades of Vogt, where the stem cells are located and where their quiescence, proliferation and differentiation are maintained in balance. Besides the epithelial stem and progenitor cell clusters, the limbal niche comprises several types of supporting niche cells and a specific extracellular matrix mediating biochemical and biophysical signals. Stem cell-based tissue engineering aims to mimic the native stem cell niche and to present appropriate microenvironmental cues in a controlled and reproducible fashion in order to maintain stem cell function within the graft. Current therapeutic approaches for ex vivo expansion of limbal stem cells only take advantage of surrogate niches. However, new insights into the molecular composition of the limbal niche and innovative biosynthetic scaffolds have stimulated novel strategies for niche-driven stem cell cultivation. Promising experimental approaches include collagen-based organotypic coculture systems of limbal epithelial stem cells with their niche cells and biomimetic hydrogel platforms prefunctionalized with appropriate biomolecular and biophysical signals. Future translation of these novel regenerative strategies into clinical application is expected to improve long-term outcomes of limbal stem cell transplantation for ocular surface reconstruction.},
author = {Schlötzer-Schrehardt, Ursula and Freudenberg, U. and Kruse, Friedrich},
doi = {10.1007/s00347-017-0468-0},
faupublication = {yes},
journal = {Ophthalmologe},
note = {EVALuna2:21332},
peerreviewed = {Yes},
title = {{The} emerging technology of tissue engineering : {Focus} on stem cell niche},
year = {2017}
}
@inproceedings{faucris.208944738,
author = {Kruse, Friedrich and Latta, Lorenz and Holbach, Leonard and Kaesmann-Kellner, Barbara and Seitz, Berthold and Schlötzer-Schrehardt, Ursula},
faupublication = {yes},
note = {EVALuna2:34736},
peerreviewed = {Yes},
title = {{The} limbal epithelial stem cell niche in aniridia-related keratopathy},
volume = {59},
year = {2018}
}
@article{faucris.229010222,
abstract = {LOXL1 (lysyl oxidase-like 1) has been identified as the major effect locus in pseudoexfoliation (PEX) syndrome, a fibrotic disorder of the extracellular matrix and frequent cause of chronic open-angle glaucoma. However, all known PEX-associated common variants show allele effect reversal in populations of different ancestry, casting doubt on their biological significance. Based on extensive LOXL1 deep sequencing, we report here the identification of a common non-coding sequence variant, rs7173049A>G, located downstream of LOXL1, consistently associated with a decrease in PEX risk (odds ratio, OR=0.63; P=6.33x10(-31)) in nine different ethnic populations. We provide experimental evidence for a functional enhancer-like regulatory activity of the genomic region surrounding rs7173049 influencing expression levels of ISLR2 (immunoglobulin superfamily containing leucine-rich repeat protein 2) and STRA6 [stimulated by retinoic acid (RA) receptor 6], apparently mediated by allele-specific binding of the transcription factor thyroid hormone receptor beta. We further show that the protective rs7173049-G allele correlates with increased tissue expression levels of ISLR2 and STRA6 and that both genes are significantly downregulated in tissues of PEX patients together with other key components of the STRA6 receptor-driven RA signaling pathway. siRNA-mediated downregulation of RA signaling induces upregulation of LOXL1 and PEX-associated matrix genes in PEX-relevant cell types. These data indicate that dysregulation of STRA6 and impaired retinoid metabolism are involved in the pathophysiology of PEX syndrome and that the variant rs7173049-G, which represents the first common variant at the broad LOXL1 locus without allele effect reversal, mediates a protective effect through upregulation of STRA6 in ocular tissues.},
author = {Berner, Daniel and Hoja, Ursula and Zenkel, Matthias and Ross, James Julian and Uebe, Steffen and Paoli, Daniela and Frezzotti, Paolo and Rautenbach, Robyn M. and Ziskind, Ari and Williams, Susan E. and Carmichael, Trevor R. and Ramsay, Michele and Topouzis, Fotis and Chatzikyriakidou, Anthi and Lambropoulos, Alexandros and Sundaresan, Periasamy and Ayub, Humaira and Akhtar, Farah and Qamar, Raheel and Zenteno, Juan C. and Cruz-Aguilar, Marisa and Astakhov, Yury S. and Dubina, Michael and Wiggs, Janey and Ozaki, Mineo and Kruse, Friedrich and Aung, Tin and Reis, André and Khor, Chiea Chuen and Pasutto, Francesca and Schlötzer-Schrehardt, Ursula},
doi = {10.1093/hmg/ddz075},
faupublication = {yes},
journal = {Human Molecular Genetics},
note = {CRIS-Team WoS Importer:2019-11-12},
pages = {2531-2548},
peerreviewed = {Yes},
title = {{The} protective variant rs7173049 at {LOXL1} locus impacts on retinoic acid signaling pathway in pseudoexfoliation syndrome},
volume = {28},
year = {2019}
}
@article{faucris.111069464,
abstract = {Basal cell carcinomas are the most common malignant tumors of the eyelids. Patient history, clinical symptoms and signs, inspection, palpation and slit-lamp examination usually allow a working diagnosis; however, the clinical diagnosis requires histopathological confirmation and determination of the histopathological type. Squamous cell carcinomas, sebaceous gland carcinomas, melanomas and Merkel cell carcinomas can metastasize usually via the lymph vessels into the regional lymph nodes. Microscopically controlled excision of the primary tumor into healthy tissue is most commonly the first goal. Palpation and ultrasonography of the regional lymph nodes and also computed tomography (CT) with contrast enhancement and magnetic resonance imaging (MRI) for tumors with perineural sheath cell invasion are necessary to define the TNM stage. Non-surgical treatment options are becoming more popular in the further management of malignant eyelid tumors.},
author = {Weiling, Max and Bergua, A. and Kruse, Friedrich and Holbach, L.},
doi = {10.1007/s00347-016-0387-5},
faupublication = {yes},
journal = {Ophthalmologe},
note = {EVALuna2:21311},
pages = {1095-1108},
peerreviewed = {Yes},
title = {{Therapy} options for malignant eyelid tumors},
volume = {113},
year = {2016}
}
@inproceedings{faucris.208944509,
author = {Sato, Masakazu and Okumura, Naoki and Nakahara, Makiko and Sato, Takahiko and Kitazawa, Koji and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich and Koizumi, Noriko},
faupublication = {yes},
note = {EVALuna2:34737},
peerreviewed = {Yes},
title = {{The} role of {TCF4} in the pathophysiology of {Fuchs} endothelial corneal dystrophy},
volume = {59},
year = {2018}
}
@article{faucris.279703426,
abstract = {The forkhead transcription factor Foxk1 is an important regulator of myogenic progenitor cells. Since our previous data from mouse retina revealed that Foxk1 is upregulated in Ptf1a-deficient mice we investigated the spatial and temporal expression of Foxk1 during development of mouse retina. Expression of Foxk1 was analyzed on both mRNA and protein level. To identify Foxk1 transcripts, retina and cerebrum (positive control) of adult animals (postnatal day 90 (P90)) was subjected to reverse transcription polymerase chain reaction (RT-PCR) and sequencing of the amplified cDNA. The Foxk1 protein was analyzed in adult retina by Western blotting and in developing eyes at embryonic day (E) 13, 15, E17, P0, P4, P7, P10 and P90 by immunohistochemistry. Localization of Foxk1 expression was determined using cell-specific markers by double labelling. Foxk1 transcripts were detected in adult retina by RT-PCR and confirmed by sequencing. Western blot analysis confirmed the expression of Foxk1 protein in the adult retina. Immunohistochemical examination of developing eyes localized the protein to bipolar, amacrine and ganglion cells with an onset of Foxk1 expression from E15 onwards. The expression pattern during development suggests that Foxk1 may have an important role in retinal cells. © 2013 Elsevier B.V. All rights reserved.},
author = {Sel, Saadettin and Muenzenberg, Christoph and Nass, Norbert and Kalinski, Thomas and Datan, Maja and Auffarth, Gerd U. and Toeteberg-Harms, Marc and Zenkel, Matthias and Kruse, Friedrich and Paulsen, Friedrich and Schicht, Martin},
doi = {10.1016/j.gep.2013.05.003},
faupublication = {yes},
journal = {Gene Expression Patterns},
keywords = {Foxk1; Retinal development; Retinogenesis; Transcription factor},
note = {CRIS-Team Scopus Importer:2022-08-05},
pages = {280-286},
peerreviewed = {Yes},
title = {{The} transcription factor {Foxk1} is expressed in developing and adult mouse neuroretina},
volume = {13},
year = {2013}
}
@article{faucris.106126064,
abstract = {To determine the time-dependent toxicity of Trypan blue at 0.06% concentration in cultured human trabecular meshwork cells.Human trabecular meshwork cells cultured in vitro were exposed to Trypan blue and acute toxicity was evaluated. Cells were exposed for 5, 30, 60 s and for 5, 15, 30, 60 min to a commercially available Trypan blue preparation (Vision Blue; DORC International Zuidland, The Netherlands). Morphology was observed by phase-contrast microscopy and cell viability was measured using Hoechst 33342 (Intergen; Purchase, NY, USA) and propidium iodide assays to determine the percentage of living and dead cells.Morphological changes occurred mainly after 5 min of exposure to Trypan blue. Viability was 96.0% ± 3.6%, 94.8% ± 3.5% and 92.5% ± 4.4% after 5, 30 and 60 s of exposure, respectively; a significant toxic effect of Trypan blue was observed after 5, 15, 30 and 60 min of exposure with a viability of 85.0% ± 3.7%, 77.0% ± 6.7%, 70.8% ± 5.9% and 68.3% ± 8.7%, respectively.Trypan blue is not toxic, in terms of cell viability, over an exposure time of up to 60 s; however, further exposure results in a gradual increase in damage of cultured human trabecular meshwork cells.},
author = {Tsaousis, Konstantinos and Kopsachilis, Nikolaos and Tsinopoulos, Ioannis T. and Dimitrakos, Stavros A. and Kruse, Friedrich and Welge-Luessen, Ulrich},
doi = {10.1111/ceo.12018},
faupublication = {yes},
journal = {Clinical and Experimental Ophthalmology},
note = {EVALuna2:21109},
pages = {484-90},
peerreviewed = {Yes},
title = {{Time}-dependent morphological alterations and viability of cultured human trabecular cells after exposure to {Trypan} blue},
volume = {41},
year = {2013}
}
@article{faucris.208944278,
abstract = {Understanding transcription factor (TF) regulation of limbal epithelial stem/progenitor cells (LEPCs) may aid in using non-ocular cells to regenerate the corneal surface. This study aimed to identify and characterize TF genes expressed specifically in LEPCs isolated from human donor eyes by laser capture microdissection. Using a profiling approach, preferential limbal expression was found for SoxE and SoxF genes, particularly for Sox9, which showed predominantly cytoplasmic localization in basal LEPCs and nuclear localization in suprabasal and corneal epithelial cells, indicating nucleocytoplasmic translocation and activation during LEPC proliferation and differentiation. Increased nuclear localization of Sox9 was also observed in activated LEPCs following clonal expansion and corneal epithelial wound healing. Knockdown of SOX9 expression in cultured LEPCs by RNAi led to reduced expression of progenitor cell markers, e.g. keratin 15, and increased expression of differentiation markers, e.g. keratin 3. Furthermore, SOX9 silencing significantly suppressed the proliferative capacity of LEPCs and reduced levels of glycogen synthase kinase 3 beta (GSK-3ß), a negative regulator of Wnt/ß-catenin signaling. Sox9 expression, in turn, was significantly suppressed by treatment of LEPCs with exogenous GSK-3ß inhibitors and enhanced by small molecule inhibitors of Wnt signaling. Our results suggest that Sox9 and Wnt/ß-catenin signaling cooperate in mutually repressive interactions to achieve a balance between quiescence, proliferation and differentiation of LEPCs in the limbal niche. Future molecular dissection of Sox9-Wnt interaction and mechanisms of nucleocytoplasmic shuttling of Sox9 may aid in improving the regenerative potential of LEPCs and the reprogramming of non-ocular cells for corneal surface regeneration.},
author = {Menzel-Severing, Johannes and Zenkel, Matthias and Polisetti, Naresh and Sock, Elisabeth and Wegner, Michael and Kruse, Friedrich and Schlötzer-Schrehardt, Ursula},
doi = {10.1038/s41598-018-28596-3},
faupublication = {yes},
journal = {Scientific Reports},
note = {EVALuna2:34733},
peerreviewed = {Yes},
title = {{Transcription} factor profiling identifies {Sox9} as regulator of proliferation and differentiation in corneal epithelial stem/progenitor cells},
volume = {8},
year = {2018}
}
@inproceedings{faucris.118320004,
author = {Stafiej, Piotr and Küng, Florian and Kruse, Friedrich and Schubert, Dirk W. and Fuchsluger, Thomas},
booktitle = {Association for Research in Vision and Ophthalmology},
faupublication = {yes},
peerreviewed = {unknown},
title = {{Transparency} of nanofiber reinforced alginate hydrogels for corneal wound healing},
venue = {Baltimore},
year = {2017}
}
@article{faucris.117887264,
abstract = {This retrospective study reports on four patients with severe recurrent symblepharopterygium formation and extensive subconjunctival scarring who were treated with a novel surgical technique combining free limbal autografting and amniotic membrane transplantation.The surgical technique included symblepharolysis, meticulous removal of subconjunctival scar tissue, ipsilateral free limbal autograft and homologous amniotic membrane transplantation.There were no intraoperative or postoperative adverse events and three patients had no manifestation of recurrence of pterygium, symblepharon or diplopia during a mean follow-up period of 172 ± 18 weeks (39 ± 4 months) postoperatively. Only one patient had persistent symblepharon and experienced a recurrence of pterygium approximately 40 weeks (9 months) after surgery.The results suggest that ipsilateral autologous limbal and homologous amniotic membrane transplantation can be an effective therapeutic approach in the treatment of recurrent pterygium with symblepharon formation.},
author = {Huchzermeyer, Cord and Gatzioufas, Z. and Kruse, Friedrich and Seitz, B.},
doi = {10.1007/s00347-013-2979-7},
faupublication = {yes},
journal = {Ophthalmologe},
note = {EVALuna2:21026},
pages = {839-45},
peerreviewed = {Yes},
title = {{Treatment} of severe recurrent symblepharopterygium: combined ipsilateral autologous limbus and homologous amniotic membrane transplantation},
volume = {111},
year = {2014}
}
@article{faucris.119503384,
abstract = {Purpose. To analyze whether tumor-associated lymphangiogenesis is concurrent with the progression of premalignant conjunctival melanocytic intraepithelial neoplasia (C-MIN) into invasive conjunctival melanoma (CM) and to study its association with prognosis. Methods. Twenty patients with CM were closely matched with 20 patients with C-MIN with atypia and 20 with C-MIN without atypia regarding tumor size, tumor location, tumor extension, and patient's age. All conjunctival specimens were analyzed for the immunohistochemical presence of proliferating lymphatic vessels, with LYVE-1 and podoplanin used as specific lymphatic endothelial markers and Ki-67 as a proliferation marker. Lymphatic vascular density was measured within the mass (intratumoral) and within an area <=500 ?m from the tumor border (peritumoral) and was correlated with recurrence, metastasis, and survival rates. Results. Intratumoral and peritumoral proliferating lymphatic vessels were detected in none of the C-MINs without atypia, in 10 of the 20 C-MINs with atypia, and in all 20 CMs. Invasive CM showed a significantly higher intra- and peritumoral density of proliferating lymphatics than did C-MIN with atypia (P <= 0.001). Patients with high intratumoral lymphatic density revealed significantly lower recurrence-free survival rates (P = 0.041) in C-MIN with atypia and significantly lower recurrence-free (P = 0.006), lymphatic-spread-free (P = 0.041), distant-metastasis-free (P = 0.029), and melanoma-specific survival rates (P = 0.029) in CM. Conclusions. Development of CM from premalignant precursors is concurrent with the outgrowth of lymphatic vessels. This active lymphangiogenesis seems to be associated with an increased risk of local recurrence in patients with C-MIN with atypia and with an increased risk of local recurrence, lymphatic spread, distant metastasis, and tumor-related death in patients with invasive CM.},
author = {Heindl, Ludwig M. and Hofmann-Rummelt, Carmen and Adler, Werner and Bosch, Jacobus J. and Holbach, Leonard M. and Naumann, Gottfried and Kruse, Friedrich and Cursiefen, Claus},
doi = {10.1167/iovs.11-7902},
faupublication = {yes},
journal = {Investigative Ophthalmology & Visual Science},
note = {EVALuna2:5320},
pages = {7074-83},
peerreviewed = {Yes},
title = {{Tumor}-associated lymphangiogenesis in the development of conjunctival melanoma},
volume = {52},
year = {2011}
}
@article{faucris.308936996,
abstract = {PURPOSE. There is a pressing need to investigate the impact of type II diabetes mellitus on the posterior cornea in donor tissues given its increasing prevalence and potential impact on endothelial keratoplasty surgical outcomes. METHODS. Immortalized human cultured corneal endothelial cells (CECs; HCEC-B4G12) were grown in hyperglycemic media for 2 weeks. Extracellular matrix (ECM) adhesive glycoprotein expression and advanced glycation end products (AGEs) in cultured cells and corneoscleral donor tissues, as well as the elastic modulus for the Descemet membrane (DMs) and CECs of diabetic and nondiabetic donor corneas, were measured. RESULTS. In CEC cultures, increasing hyperglycemia resulted in increased transforming growth factor beta-induced (TGFBI) protein expression and colocalization with AGEs in the ECM. In donor corneas, the thicknesses of the DM and the interfacial matrix (IFM) between the DM and stroma both increased from 8.42 ± 1.35 μm and 0.504 ± 0.13 μm in normal corneas, respectively, to 11.13 ± 2.91 μm (DM) and 0.681 ± 0.24 μm (IFM) in non-advanced diabetes (P = 0.013 and P = 0.075, respectively) and 11.31 ± 1.76 μm (DM) and 0.744 ± 0.18 μm (IFM) in advanced diabetes (AD; P = 0.0002 and P = 0.003, respectively). Immunofluorescence in AD tissues versus controls showed increased AGEs (P < 0.001) and markedly increased labeling intensity for adhesive glycoproteins, including TGFBI, that colocalized with AGEs. The elastic modulus significantly increased between AD and control tissues for the DMs (P < 0.0001) and CECs (P < 0.0001). CONCLUSIONS. Diabetes and hyperglycemia alter human CEC ECM structure and composition, likely contributing to previously documented complications of endothelial keratoplasty using diabetic donor tissue, including tearing during graft preparation and reduced graft survival. AGE accumulation in the DM and IFM may be a useful biomarker for determining diabetic impact on posterior corneal tissue.},
author = {Kingsbury, Kenten D. and Skeie, Jessica M. and Cosert, Krista and Schmidt, Gregory A. and Aldrich, Benjamin T. and Sales, Christopher S. and Weller, Julia and Kruse, Friedrich and Thomasy, Sara M. and Schlötzer-Schrehardt, Ursula and Greiner, Mark A.},
doi = {10.1167/iovs.64.7.26},
faupublication = {yes},
journal = {Investigative Ophthalmology & Visual Science},
keywords = {corneal biomechanics; corneal endothelial cells; corneal transplantation},
note = {CRIS-Team Scopus Importer:2023-08-11},
peerreviewed = {Yes},
title = {{Type} {II} {Diabetes} {Mellitus} {Causes} {Extracellular} {Matrix} {Alterations} in the {Posterior} {Cornea} {That} {Increase} {Graft} {Thickness} and {Rigidity}},
volume = {64},
year = {2023}
}
@article{faucris.121521884,
abstract = {To reinvestigate the ultrastructure of the posterior stroma of the human cornea and to correlate the findings with the stromal behavior after big-bubble creation.Observational consecutive 3-center case series.Fresh corneoscleral buttons from human donors (n = 19) and organ-cultured corneoscleral buttons (n = 10) obtained after Descemet's membrane endothelial keratoplasty.Corneal specimens were divided into central (3 mm), mid peripheral (8 mm), and peripheral parts by trephination and processed for transmission electron microscopic and immunohistochemical analyses. A big bubble was created by air injection into the stroma of organ-cultured corneas before fixation.The distance of keratocytes to Descemet's membrane, number of collagen lamellae between keratocytes and Descemet's membrane, diameter and arrangement of collagen fibrils, thickness of stromal lamella created by air injection, and immunopositivity for collagen types III, IV, and VI.Stromal keratocytes were observed at variable distances from Descemet's membrane, increasing from 1.5 to 12 ?m (mean, 4.97±2.19 ?m) in the central, 3.5 to 14 ?m (mean, 8.03±2.47 ?m) in the midperipheral, and 4.5 to 18 ?m (mean, 9.77±2.90 ?m) in the peripheral regions. The differences in mean distances were significant (P < 0.0001). The number of collagen lamellae between Descemet's membrane and most posterior keratocytes varied from 2 to 10 and the diameter of collagen fibrils averaged 23.5±1.8 nm and corresponded with that of the remaining stroma. A thin layer (0.5-1.0 ?m thick) of randomly arranged, unaligned collagen fibers, which was positive for collagen types III and VI, was observed at the Descemet-stroma interface. The residual stromal sheet separated by air injection in 8 of 10 donor corneas varied in thickness from 4.5 to 27.5 ?m, even within individual corneas (<=3-fold), and was composed of 5 to 11 collagen lamellae that revealed keratocytes on their anterior surface and in between.Barring an anchoring zone of interwoven collagen fibers at the Descemet-stroma interface, the findings did not provide any evidence for the existence of a distinctive acellular pre-Descemet's stromal layer in the human cornea. The intrastromal cleavage plane after pneumodissection seems to be nonreproducibly determined by the intraindividually and interindividually variable distances of keratocytes to Descemet's membrane.},
author = {Schlötzer-Schrehardt, Ursula and Bachmann, Bjoern O. and Tourtas, Theofilos and Torricelli, Andre A. M. and Singh, Arun and Gonzalez, Sheyla and Mei, Hua and Deng, Sophie X. and Wilson, Steven E. and Kruse, Friedrich},
doi = {10.1016/j.ophtha.2014.09.037},
faupublication = {yes},
journal = {Ophthalmology},
note = {EVALuna2:21206},
pages = {693-9},
peerreviewed = {Yes},
title = {{Ultrastructure} of the posterior corneal stroma},
volume = {122},
year = {2015}
}
@article{faucris.119511964,
author = {Berta, A. I. and Naumann-Bartsch, N. and Agaimy, Abbas and Metzler, Markus and Kruse, Friedrich and Holbach, L.},
doi = {10.1007/s00347-013-2881-3},
faupublication = {yes},
journal = {Ophthalmologe},
note = {EVALuna2:6506},
pages = {876-8},
peerreviewed = {Yes},
title = {{Unilateral} swelling of the lacrimal gland in a 15-year-old female patient},
volume = {110},
year = {2013}
}
@article{faucris.119816664,
abstract = {To describe the use of an accidentally torn Descemet membrane (DM) to successfully complete Descemet membrane endothelial keratoplasty (DMEK) surgery.Retrospective, observational case series of 3 eyes of 3 patients undergoing DMEK with a DM accidentally torn into 2 pieces during graft preparation. The mean outcome measures included best-corrected visual acuity, endothelial cell density, and central corneal thickness, before and at 1, 3, and 6 months after the DMEK surgery was performed.During graft preparation, immediately before transplantation, a large tear within the 8.0-mm marking line of the DM occurred, resulting in a DM torn into 2 pieces. In all the eyes, both pieces were successfully implanted into the anterior chamber, unfolded and attached to the posterior corneal stroma, one after the other. Six months after the surgery was performed, the best-corrected visual acuity ranged between 20/30 and 20/25. Endothelial cell loss was about 30% (range 28%-32%) 6 months after the surgery. Pachymetry findings showed normal corneal thickness 6 months after the surgery. All corneas remained clear without any signs of graft failure within 6 months of follow-up.DMEK surgery can be successfully completed despite the accidental tearing of donor DMs during the preparation of DMEK grafts by the sequential implantation of both DM pieces.},
author = {Tourtas, Theofilos and Heindl, Ludwig M. and Kopsachilis, Nikolaos and Bachmann, Bjoern O. and Kruse, Friedrich and Cursiefen, Claus},
doi = {10.1097/ICO.0b013e3182a6ea4f},
faupublication = {yes},
journal = {Cornea},
note = {EVALuna2:21133},
pages = {1418-22},
peerreviewed = {Yes},
title = {{Use} of accidentally torn descemet membrane to successfully complete descemet membrane endothelial keratoplasty},
volume = {32},
year = {2013}
}
@article{faucris.106524924,
abstract = {The aim of this study was to verify the formation of hydroxyl radicals (·OH) after ultraviolet A (UVA) irradiation of riboflavin (RF) by spin trapping with 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), and electron spin resonance spectroscopy.We found that ·OH were generated via hydrogen peroxide (H?O?) formation during UVA irradiation of RF. The ·OH radicals were trapped with DMPO yielding 2-hydroxy-5,5-dimethyl-1-pyrroline-N-oxide (·DMPO-OH). The formed radical adduct (·DMPO-OH) accumulated in the RF solution. Argon equilibration of the RF solution completely blocked the formation of the ·DMPO-OH adduct whereas subsequent aeration restored radical adduct generation. The presence of catalase inhibited ·DMPO-OH generation whereas BSA had no influence on ·DMPO-OH formation. Stopping UVA irradiation led to decay of radical adducts. UVA irradiation of H?O? in the presence of DMPO but without RF also induced the formation of ·DMPO-OH adduct. When adding DMPO to an already irradiated RF solution significantly less ·DMPO-OH was formed during further irradiation. Ultraviolet-visible spectroscopy and high-performance liquid chromatography analysis of RF indicated that RF decayed during UVA irradiation.The formation of ·OH during UVA irradiation of RF may be part of the oxygen-dependent mechanism involved in the cross-linking therapy of collagen in corneal stroma.},
author = {Sel, Saadettin and Nass, Norbert and Poetzsch, Sandy and Trau, Stefanie and Simm, Andreas and Kalinski, Thomas and Duncker, Gernot I. W. and Kruse, Friedrich and Auffarth, Gerd U. and Broemme, Hans-Juergen},
doi = {10.1179/1351000213Y.0000000076},
faupublication = {yes},
journal = {Redox Report},
note = {EVALuna2:21207},
pages = {72-9},
peerreviewed = {Yes},
title = {{UVA} irradiation of riboflavin generates oxygen-dependent hydroxyl radicals},
volume = {19},
year = {2014}
}
@article{faucris.210427049,
abstract = {PURPOSE: To characterize corneal wound healing in a rabbit model after flapless refractive lenticule extraction with a 345 nm ultraviolet femtosecond laser.
SETTING: Departments of Ophthalmology and Anatomy II, University of Erlangen-Nürnberg and Wavelight GmbH, Erlangen, Germany.
DESIGN: Experimental study.
METHODS: Flapless refractive lenticule extraction was performed in 1 eye each of 20 New Zealand white rabbits (-5.0 diopters). Groups of 4 animals were euthanized after 48 hours, 1 week, 2 weeks, 4 weeks, and 3 months, respectively. Corneal samples were prepared for histology and fluorescence microscopy. To assess corneal cell death, proliferation, and myofibroblastic transdifferentiation, terminal uridine deoxynucleotidyl nick end-labeling (TUNEL) assay as well as immunostaining for Ki67 and α-smooth muscle actin (αSMA) were performed on sagittal cryosections.
RESULTS: Histology revealed a zone of keratocyte depletion with a thickness of approximately 50 μm around the extraction site. At 48 hours, pronounced TUNEL staining of keratocytes was detected around the interface (159.9 cells/mm ± 18.4 [SD]), which steadily decreased to 74.9 ± 19.8 cells/mm at 1 week and 5.7 ± 4.8 cells/mm at 2 weeks. Ki67 staining of keratocytes was evident at 48 hours (10.0 ± 3.8 cells/mm), which then decreased at 1 week (5.2 ± 1.7 cells/mm) and 2 weeks (0.4 ± 0.5 cells/mm). From 4 weeks onward, no TUNEL or Ki67 staining was detected. The corneal stroma was αSMA-negative at all timepoints.
CONCLUSION: Application of the 345 nm laser showed no signs of problematic repair processes in the cornea, which supports the initiation of the clinical phase.},
author = {Hammer, Christian M. and Petsch, Corinna and Klenke, Joerg and Skerl, Katrin and Wuellner, Christian and Donitzky, Christof and Paulsen, Friedrich and Scholz, Michael and Seiler, Theo and Kruse, Friedrich and Menzel-Severing, Johannes},
doi = {10.1016/j.jcrs.2017.07.034},
faupublication = {yes},
journal = {Journal of Cataract and Refractive Surgery},
note = {EVALuna2:35326},
pages = {1335-1342},
peerreviewed = {Yes},
title = {{Wound} healing in rabbit corneas after flapless refractive lenticule extraction with a 345 nm ultraviolet femtosecond laser},
volume = {43},
year = {2017}
}
@article{faucris.110299244,
abstract = {We compared cell number, putative stem cell markers, and clonogenic ability in fresh uncultured human limbal epithelial cells to that obtained from stored organ-cultured tissue.Cell suspensions were formed from fresh and organ culture-stored human limbal epithelium. Expression of putative stem cell markers ?Np63 and TrkA was performed using immunofluorescent staining before culture. Colony-forming efficiency (CFE) assays were performed at first passage. The effects of tissue storage, age, and postmortem/culture times were analyzed in a general linear model.Limbal tissue from 94 donors (34 fresh and 60 stored) was compared. Three times more cells were obtained per eye from fresh (35.34 × 104; SD, 17.39) than stored (11.24 × 104; SD, 11.57; P < 0.01) tissue. A higher proportion of cells from fresh tissue were viable (91.9%; SD, 5.7 vs. 85%; SD, 10.8) P < 0.01. Higher total cell expression of ?Np63 (20.19 × 104; SD, 15.5 vs. 3.28 104; SD, 4.33) and TrkA (59.24 × 104; SD, 13.21 vs. 7.65 × 104; SD, 1.05) was observed in fresh than stored tissue (P < 0.01). Colony-forming efficiency was higher for fresh (1.42; SD, 0.12) than stored (0.43; SD, 0.15; P < 0.01) cells. For stored tissue only, there was a significant inverse relationship between donor age and total number of cells isolated (R2 = 0.27, P < 0.001).Storage of corneoscleral discs in organ culture medium leads to significant reduction in limbal epithelial cell number, expression of ?Np63 and TrkA, and viability compared to fresh tissue. There is a smaller basal stem cell population in stored compared to fresh tissue.},
author = {Mason, Sharon L. and Stewart, Rosalind M. K. and Sheridan, Carl M. and Keshtkar, Fatemeh and Rooney, Paul and Austin, Eric and Schlötzer-Schrehardt, Ursula and Kruse, Friedrich and Kaye, Stephen B.},
doi = {10.1167/iovs.16-19354},
faupublication = {yes},
journal = {Investigative Ophthalmology & Visual Science},
note = {EVALuna2:21288},
pages = {3708-13},
peerreviewed = {Yes},
title = {{Yield} and {Viability} of {Human} {Limbal} {Stem} {Cells} {From} {Fresh} and {Stored} {Tissue}},
volume = {57},
year = {2016}
}